DOCSLIB.ORG

16Th International Congress of the Intestinal Rehabilitation And

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
16Th International Congress of the Intestinal Rehabilitation And CIRTA 2019 16th International Congress of the Intestinal Rehabilitation & Transplant Association Paris, July 3-6 BOOK OF ABSRACTS Intestinal Rehabilitation & Transplant ASSOCI A T ION Beaujon CIRTA 2019 Book of Abstracts – Table of Content Table of Content ORAL PRESENTATIONS ................................................................................................................. 14 200.4 - Serial Transverse Enteroplasty (STEP) for the Short Gut Syndrome (SGS) Patients with Gut Failure (GF) ........................................................................................................................................................................................ 15 210.4 - Dynamic repopulation, phenotypic evolution and clonal distribution of recipient B cells and plasma cells in graft mucosa associated with rejection after human intestinal transplantation ...................................... 15 220.4 - Results of Medical and Surgical Rehabilitation of Adult Patients with Type III Intestinal Failure in a comprehensive unit: Is it possible to predict intestinal rehabilitation?.................................................................... 16 220.5 - Diagnosis and management of congenital enteropathies: analysis of a large multi-center cohort and the role of Next Generation Sequencing. ....................................................................................................................... 17 220.6 - Effective radiation dose and bone marrow radiation exposure as a result of radiological investigation in a cohort of multivisceral transplant patients ....................................................................................18 220.7 - Human intestinal tissue-resident memory CD8 T cells comprise two transcriptionally distinct populations ..........................................................................................................................................................................18 230.5 - Washington D.C. single-center experience in adult and pediatric intestinal transplantation ............... 19 230.6 - Multivisceral Transplant without an ostomy – 5-year experience ...........................................................20 230.7 - Disease Recurrence After Intestinal and Multivisceral Transplantation ................................................... 21 250.5 - Early Predictors of Enteral Autonomy in Pediatric Intestinal Failure: Development of a Disease Severity Score ...................................................................................................................................................................... 21 250.6 - Results of a Multicentric Retrolective Study of Teduglutide Treatment in Benign Short Bowel Syndrome in Germany ....................................................................................................................................................... 22 250.7 - Impact of donor selection on early graft loss in Intestinal Transplantation. ........................................... 23 260.5 - An algorithm for progress from parenteral nutrition to oral nutrition for adult intestinal transplant recipients ............................................................................................................................................................................. 23 260.6 - Pediatric Home Parenteral Nutrition (Hpn) In France: A National Survey On The Behalf Of The French Pediatric Hpn Network ........................................................................................................................................ 24 260.7 - Effective transitioning of adolescents into adult intestinal transplant services – survey of NITE members .............................................................................................................................................................................. 24 300.5 - Milk fat globule epidermal growth factor-8 (MFG-E8) has site specific effects on intestinal lengthening in neonatal short bowel syndrome, studied in piglets ......................................................................... 25 300.6 - The REVE study, preliminary results. A Monocentric Single-arm study to characterize the long-term safety, efficacy, and pharmacodynamic of GLP-2 analog (Revestive®) in the management of short bowel syndrome pediatric patients on home-parenteral nutrition (HPN).......................................................................... 25 300.7 - Isolated intestinal transplantation: Are we shifting the paradigm? .......................................................... 27 310.5 - Early Intestinal Barrier Dysfunction Early Post Intestinal Transplantation Is Driven by the Absence of Protective Type 3 Innate Lymphoid Cells and the Persistence of Proinflammatory Type 1 Innate Lymphoid Cells ......................................................................................................................................................................................28 1 CIRTA 2019 Book of Abstracts – Table of Content 310.6 - Risks Factors For The Development Of Graft-Versus-Host Disease (Gvhd) After Intestinal And Multivisceral Transplantation ..........................................................................................................................................28 310.7 - Spleen preservation attenuates GVHD in multivisceral transplant recipients. ........................................ 29 320.1 - Changes in gut microbiota and its metabolic activity in pediatric intestinal failure patients receiving long-term parenteral nutrition ........................................................................................................................................ 29 320.2 - Extending the indications of Intestinal transplantation. Cytoreduction and modified multivisceral transplantation for patients with end-stage pseudomyxoma peritoneii. .............................................................. 30 320.3 - Ultra-Short Bowel Syndrome (USBS) – Does the lengthening procedures improve the outcomes? Intestinal Rehabilitation Program (IRP) Children’s National Medical Center (CNMC) Washington DC - USA 31 320.4 - Use of Arterial Embolisation to Facilitate Exenteration during Multi-visceral and Intestinal Transplantation ................................................................................................................................................................... 31 320.5 - The colon as an energy salvage organ for children with short bowel syndrome ................................... 32 320.6 - Potential biomarkers to guide immunosuppression management in intestinal transplant recipients ............................................................................................................................................................................................... 32 320.7 - Is there a life after surgery for acute mesenteric ischemia? A retrospective study of 184 survivors . 33 320.8 - Identification of biomarkers for risk, diagnosis, and prognosis of GVHD after bowel transplantation and central role of graft resident memory T cells in pathogenesis .......................................................................... 34 320.9 - The Double-Barrel Enteroplasty: A Novel Intestinal Lengthening Procedure for Short Bowel Syndrome ............................................................................................................................................................................ 35 320.10 - "Stoma or no stoma that is the question": a single-center experience of intestinal transplantation without stoma .....................................................................................................................................................................36 350.5 - Acute Rejection Following Intestinal Transplantation in a Cohort of 442 Transplants Performed Over Nearly 25 Years: The University of Miami Experience ................................................................................................36 350.6 - The impact of circulating DSA in visceral transplant recipients without histological evidence of rejection: How should we treat or not treat? ............................................................................................................... 37 350.7 - Post-Transplant Proliferative Disorder Following Intestine Transplantation: Contemporary Single Center Experience............................................................................................................................................................. 38 360.5 - Successul implementation of remote consultation for patients recceiving home parenteral nutrition .............................................................................................................................................................................................. 38 360.6 - Long term results of a series of pediatric patients with short bowel syndrome treated at an Intestinal Failure, Rehabilitation and Transplant Program ........................................................................................ 39 360.7 - Ex vivo tumor resection and intestinal autotransplantation – a single center 10-year experience ... 40 370.5 - Post-transplant ulcerative ileitis (PTUI): risk factors and outcomes........................................................ 40 370.6 - The Role of T helper 17 cells in Severe Intestinal Transplant Rejection Renders it a Third Form of Inflammatory Bowel Disease
Load more

Recommended publications
  • Zebrafish Models of Neurology Biochimica Et Biophysica Acta
    Biochimica et Biophysica Acta 1812 (2011) 333–334 Contents lists available at ScienceDirect Biochimica et Biophysica Acta journal homepage: www.elsevier.com/locate/bbadis Editorial Preface: Zebrafish Models of Neurology☆ Forming hypotheses about the Molecular Basis of Neurological benefitted primarily from biochemistry/ biophysics of protein Disease has traditionally been based on human genetics, biochemistry aggregation, cell culture work and mouse models; These paradigms and molecular biology, whereas testing these hypotheses requires us are challenging to implement in a fashion that tells us how disease in to turn to cell culture and animal models. Zebrafish have emerged as a one portion of the CNS spreads to another. Zebrafish are positioned tractable platform to complement and bridge these paradigms in brilliantly to fill this gap, not only because of its efficiency as a genetic many diseases, allowing integration of human genetics and cell and neuroscience model, but because zebrafish have a long history as biology within the in vivo setting. Perhaps it is in Neurology and a platform for assessing non-cell-autonomous effects of protein Neurodegeneration, however, where zebrafish have special promise perturbations in the in vivo setting. Thus it is common for zebrafish to make contributions. Four special advantages of zebrafish to biologists to ask if changing gene abundance or character in one group Neurology are worth highlighting here: efficient in vivo tests for of neurons is able to affect the fate and function of neighboring cells. genetic interactions, conserved cellular architecture of the CNS, the Creative and ambitious deployment of this ability will complement ability to create genetically mosaic animals, and tractable behaviour established tools to promise a deep understanding of how disease and electrophysiology to assess experimental interventions.
    [Show full text]
  • The Genetic and Functional Basis of Three Inherited Cutaneous and Gastro- Intestinal Diseases in Humans
    The genetic and functional basis of three inherited cutaneous and gastro- intestinal diseases in humans Matthew A. Brooke Supervisor: Professor David Kelsell A Thesis submitted for the degree of PhD Centre for Cutaneous Research, Blizard Institute Barts & The London School of Medicine & Dentistry, Queen Mary, University of London 1 I, Matthew Alexander Brooke, hereby declare that the work presented in this thesis is my own, unless otherwise stated, and is in accordance with the University of London’s regulations for the degree of PhD Matthew Alexander Brooke 2 Abstract This thesis describes investigations into the genetic basis and pathophysiology of three distinct inherited diseases in humans, two of which are strongly associated to the function of the ectodomain sheddase enzyme ADAM17. The first of these is a novel inherited syndrome of neonatal onset inflammatory skin and bowel disease, which is associated in a consanguineous family with homozygous loss-of- function mutations in ADAM17. This thesis describes investigations of the expression and function of ADAM17 – and downstream proteins it regulates – in an individual affected by this disease. This is accompanied by genetic investigations into other individuals suspected of suffering from the same syndrome. The second investigated disease is Tylosis with Oesophageal Cancer (TOC), an inherited cutaneous disease which represents the only known syndrome of familial oesophageal cancer susceptibility. This disease was associated to dominantly inherited mutations in the Rhomboid protein iRHOM2. This work describes investigations of immortalised keratinocyte cell lines and tissues derived from TOC-affected individuals, and illustrates that the pathogenesis of TOC is characterised by increased iRHOM2-dependent activation and activity of ADAM17, and upregulation of the shedding of ADAM17 substrates, particularly in the EGFR ligand family, accompanied by increased desmosome turnover and transglutaminase 1 activity.
    [Show full text]
  • Université De Montréal Caractérisation Fonctionnelle Du Gène AP1S1
    Université de Montréal Caractérisation fonctionnelle du gène AP1S1 mutant associé au syndrome de MEDNIK par Stéphanie Côté Programme de biologie moléculaire Faculté de médecine Mémoire présenté à la Faculté de médecine en vue de l’obtention d’une Maîtrise ès Sciences (M.Sc.) en biologie moléculaire Mars 2009 © Stéphanie Côté, 2009 Université de Montréal Faculté de Médecine Ce mémoire intitulé : Caractérisation fonctionnelle du gène AP1S1 mutant associé au syndrome de MEDNIK présenté par : Stéphanie Côté a été évalué par un jury composé des personnes suivantes : Gilbert Bernier Président-rapporteur Patrick Cossette Directeur de recherche Louis St-Amant Membre du jury iii RÉSUMÉ Dans les cellules eucaryotes, le trafic intracellulaire de nombreuses protéines est assuré par des vésicules de transport tapissées de clathrine. Les complexes adaptateurs de clathrine (AP) sont responsables de l’assemblage de ces vésicules et de la sélection des protéines qui seront transportées. Nous avons étudié cinq familles atteintes du syndrome neurocutané MEDNIK qui est caractérisé par un retard mental, une entéropathie, une surdité, une neuropathie périphérique, de l’icthyose et de la kératodermie. Tous les cas connus de cette maladie à transmission autosomique récessive sont originaires de la région de Kamouraska, dans la province de Québec. Par séquençage direct des gènes candidats, nous avons identifié une mutation impliquant le site accepteur de l’épissage de l’intron 2 du gène codant pour la sous- unité 1 du complexe AP1 (AP1S1). Cette mutation fondatrice a été retrouvée chez tous les individus atteints du syndrome MEDNIK et altère l’épissage normal du gène, menant à un codon stop prématuré.
    [Show full text]
  • Wilson's Disease: Facing the Challenge of Diagnosing a Rare
    biomedicines Review Wilson’s Disease: Facing the Challenge of Diagnosing a Rare Disease Ana Sánchez-Monteagudo 1,2, Edna Ripollés 1,2, Marina Berenguer 2,3,4,5,† and Carmen Espinós 1,2,*,† 1 Rare Neurodegenerative Diseases Laboratory, Centro de Investigación Príncipe Felipe (CIPF), 46012 Valencia, Spain; [email protected] (A.S.-M.); [email protected] (E.R.) 2 Joint Unit on Rare Diseases CIPF-IIS La Fe, 46012 Valencia, Spain; [email protected] 3 Hepatology-Liver Transplantation Unit, Digestive Medicine Service, IIS La Fe and CIBER-EHD, Hospital Universitari i Politècnic La Fe, 46026 Valencia, Spain 4 Department of Medicine, Universitat de València, 46010 Valencia, Spain 5 Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, CIBERehd, Instituto de Salud Carlos III, 28029 Madrid, Spain * Correspondence: [email protected]; Tel.: +34-963289680 † These authors have contributed equally. Abstract: Wilson disease (WD) is a rare disorder caused by mutations in ATP7B, which leads to the defective biliary excretion of copper. The subsequent gradual accumulation of copper in different organs produces an extremely variable clinical picture, which comprises hepatic, neurological psychi- atric, ophthalmological, and other disturbances. WD has a specific treatment, so that early diagnosis is crucial to avoid disease progression and its devastating consequences. The clinical diagnosis is based on the Leipzig score, which considers clinical, histological, biochemical, and genetic data. However, even patients with an initial WD diagnosis based on a high Leipzig score may harbor other conditions that mimic the WD’s phenotype (Wilson-like). Many patients are diagnosed using current Citation: Sánchez-Monteagudo, A.; available methods, but others remain in an uncertain area because of bordering ceruloplasmin levels, Ripollés, E.; Berenguer, M.; Espinós, inconclusive genetic findings and unclear phenotypes.
    [Show full text]
  • Classification and Differential Diagnosis of Wilson's Disease
    63 Review Article Page 1 of 14 Classification and differential diagnosis of Wilson’s disease Wieland Hermann Department of Neurology, SRO AG Spital Langenthal, Langenthal, Switzerland Correspondence to: Prof. Dr. habil. Wieland Hermann, MD. Chief Physician of Neurology, SRO AG Spital Langenthal, St. Urbanstrasse 67, CH - 4900 Langenthal, Switzerland. Email: [email protected]. Abstract: Wilson’s disease is characterized by hepatic and extrapyramidal movement disorders (EPS) with variable manifestation primarily between age 5 and 45. This variability often makes an early diagnosis difficult. A classification defines different clinical variants of Wilson’s disease, which enables classifying the current clinical findings and making an early tentative diagnosis. Until the unequivocal proof or an autosomal recessive disorder of the hepatic copper transporter ATP7B has been ruled out, differential diagnoses have to be examined. Laboratory-chemical parameters of copper metabolism can both be deviations from the norm not related to the disease as well as other copper metabolism disorders besides Wilson’s disease. In addition to known diseases such as Menkes disease, occipital horn syndrome (OHS), Indian childhood cirrhosis (ICC) and ceruloplasmin deficiency, recently discovered disorders are taken into account. These include MEDNIK syndrome, Huppke-Brendel syndrome and CCS chaperone deficiency. Another main focus is on differential diagnoses of childhood icterus correlated with age and anaemia as well as disorders of the extrapyramidal motor system. The Kayser-Fleischer ring (KFR) is qualified as classical ophthalmologic manifestation. The recently described manganese storage disease presents another rare metabolic disorder with symptoms similar to Wilson’s disease. As this overview shows, Wilson’s disease fits into a broad spectrum of internal and neurological disease patterns with icterus, anaemia and EPS.
    [Show full text]
  • NASPGHAN Annual Mtg 2017
    NASPGHAN 2017 Annual Meeting & Postgraduate Course NASPGHAN 2017 Annual Meeting & Postgraduate Course Annual Meeting Program NASPGHAN 2017 NASPGHAN 2017 2017 NASPGHANAnnual Meeting & Postgraduate Course ANNUALAnnual Meeting & Postgraduate CourseMEETING November 1– 4, 2017 Caesars Palace Las Vegas, NV TABLE OF CONTENTS PROGRAM AT A GLANCE ................................................................................................................................... 3 GENERAL INFORMATION ................................................................................................................................... 5 MEETING SUPPORT .................................................................................................................................... 8 FUTURE DATES .................................................................................................................................... 9 TEACHING AND TOMORROW ............................................................................................................................ 10 COMMITTEE MEETINGS .................................................................................................................................... 11 SATELLITE SYMPOSIA .................................................................................................................................... 12 Thursday, November 2, 2017 POSTER SESSION I/WELCOME RECEPTION/EXHIBITS ........................................................................................ 15 Friday, November
    [Show full text]
  • Clinical Vignette Abstracts NASPGHAN 2017
    Clinical Vignette Abstracts NASPGHAN 2017 Poster Session I Thursday, November 2, 2017 5:00pm – 7:00pm 1 SOLITARY RECTAL ULCER SYNDROME IN A TEENAGER REQUIRING MULTIPLE BLOOD TRANSFUSIONS. A.M. McClain, J. Pohl, R.A. Patel, A. Lowichik, K. Jensen, Pediatric Gastroenterology, Hepatology & Nutrition, University of Utah, Salt Lake City, Utah, UNITED STATES. A previously healthy 17-year-old female presented to an outside hospital after two days of bloody diarrhea, stooling urgency and rectal bleeding. She was found to have a hemoglobin of 6 g/dL and received a blood transfusion. Over the next 8 months, similar symptoms occurred four more times, requiring two more blood transfusions. The patient was referred to our GI clinic and underwent an extensive evaluation including upper endoscopy, colonoscopy on two separate occasions, Meckel’s scan, capsule endoscopy and CT angiography.On initial colonoscopy she had a sessile appearing lesion on the left wall of the rectum. Pathology of the rectal lesion showed replacement of lamina propria by collagen and smooth muscle bundles and distortion of the crypts, consistent with solitary rectal ulcer syndrome [SRUS]. She was started on a bowel program. Due to continued bleeding, CT angiography was done and showed nonspecific thickening of the left lateral sidewall of rectum with increased vascularity. Repeat colonoscopy seven months later demonstrated a deep rectal ulcer on the left side of rectum; pathology showed hyperplastic epithelium and crypt distortion again with fibrosis in the lamina propria. Surgical consultation believed this large, worsening ulcer was the cause of recurrent bleeding. Surgical resection at that time was felt to be extremely challenging and a more intensive laxative bowel program recommended with surgical follow up for possible defecography.SRUS has been well described in the adult literature with several theories to explain the pathophysiology.
    [Show full text]
  • Disorders of Metal Metabolism Carlos Ferreira George Washington University
    Himmelfarb Health Sciences Library, The George Washington University Health Sciences Research Commons Pediatrics Faculty Publications Pediatrics 2017 Disorders of metal metabolism Carlos Ferreira George Washington University William A. Gahl Follow this and additional works at: https://hsrc.himmelfarb.gwu.edu/smhs_peds_facpubs Part of the Nutritional and Metabolic Diseases Commons, and the Pediatrics Commons APA Citation Ferreira, C., & Gahl, W. A. (2017). Disorders of metal metabolism. Translational Science of Rare Diseases, 2 (3-4). http://dx.doi.org/ 10.3233/TRD-170015 This Journal Article is brought to you for free and open access by the Pediatrics at Health Sciences Research Commons. It has been accepted for inclusion in Pediatrics Faculty Publications by an authorized administrator of Health Sciences Research Commons. For more information, please contact [email protected]. Translational Science of Rare Diseases 2 (2017) 101–139 101 DOI 10.3233/TRD-170015 IOS Press Review Article Disorders of metal metabolism Carlos R. Ferreiraa,b,c,∗ and William A. Gahlc aDivision of Genetics and Metabolism, Children’s National Health System, Washington, DC, USA bDepartment of Pediatrics, George Washington University School of Medicine and Health Sciences, Washington, DC, USA cSection on Human Biochemical Genetics, Medical Genetics Branch, National Human Genome Research Institute, NIH, Bethesda, MD, USA Abstract. Trace elements are chemical elements needed in minute amounts for normal physiology. Some of the physiologically relevant trace elements include iodine, copper, iron, manganese, zinc, selenium, cobalt and molybdenum. Of these, some are metals, and in particular, transition metals. The different electron shells of an atom carry different energy levels, with those closest to the nucleus being lowest in energy.
    [Show full text]
  • MEDNIK Syndrome: a New Entry in the Spectrum of Inborn Errors of Copper Metabolism
    www.jpnim.com Open Access eISSN: 2281-0692 Journal of Pediatric and Neonatal Individualized Medicine 2020;9(2):e090202 doi: 10.7363/090202 Received: 2020 Apr 23; accepted: 2020 May 03; published online: 2020 May 14 Editorial MEDNIK syndrome: a new entry in the spectrum of inborn errors of copper metabolism Gavino Faa1,2, Clara Gerosa1, Massimo Castagnola3 1Institute of Pathology, Department of Medical Sciences and Public Health, AOU Cagliari, University of Cagliari, Cagliari, Italy 2Temple University, Philadelphia, PA, USA 3Laboratory of Proteomics, IRCSS Fondazione Santa Lucia, Rome, Italy “There is nothing Either Good or bad But thinking Makes it so” William Shakespeare, Hamlet, II, II Keywords MEDNIK syndrome, inborn errors, copper, metabolism. Corresponding author Clara Gerosa, Divisione di Anatomia Patologica, Dipartimento di Scienze Mediche e Sanità Pubblica, AOU Cagliari, University of Cagliari, Cagliari, Italy; email: [email protected]. How to cite Faa G, Gerosa C, Castagnola M. MEDNIK syndrome: a new entry in the spectrum of inborn errors of copper metabolism. J Pediatr Neonat Individual Med. 2020;9(2):e090202. doi: 10.7363/090202. 1/5 www.jpnim.com Open Access Journal of Pediatric and Neonatal Individualized Medicine • vol. 9 • n. 2 • 2020 Copper homeostasis, including intestinal absorp­ copper metabolism, including liver copper storage tion, blood transport, uptake, trafficking, and associated with intrahepatic cholestasis and low excretion, is regulated by multiple genes encoding copper serum levels [12]. The similarities between for specific copper transporters, which coordinate MEDNIK syndrome and Wilson disease were copper bioavailability [1]. Mutations in genes coding confirmed by the finding in the same subject of for copper pumps or copper chaperons are responsible low ceruloplasmin serum levels.
    [Show full text]
  • Table 2: Genetic Classification of the Syndromic Inherited Neuropathies
    Table 2: Genetic classification of the syndromic inherited neuropathies. The table lists neuropathies that have been found in various syndromes. Neuropathies are grouped by their pattern of inheritance and phenotypes, some diseases are thus listed more than once. The genes are named according to the HUGO gene nomenclature (http://www.genenames.org/), and hyperlinked to this database. The diseases, their chromosomal locus, and genes are hyperlinked to OMIM (http://www.ncbi.nlm.nih.gov/Omim/). disease (OMIM) gene (OMIM) clinical syndromic neuropathies with slow conduction velocities and/or dysmyelinating/demyelinating neuropathies Potocki-Lupski syndrome Contiguous gene developmental hypotonia, failure to thrive, mental retardation, pervasive (610883) duplication syndrome developmental disorders, congenital anomalies including PMP22 Waardenburg syndrome SOX10 (602229) hypopigmentation,deafness, , hypogonadotropic hypogonadism, anosmia, type 2E (611584) agenesis of the olfactory bulbs PCWH (609136) SOX10 (602229) CNS and PNS, Hirschsprung disease Cowden syndrome 1 PTEN (601728) a man with multifocal motor neuropathy since childhood, learning (158350) difficulties, cranial neuropathies had a de novo PTEN mutation metachromatic ARSA (607574) optic atrophy, mental retardation, hypotonia; possibly treatable with bone leukodystrophy (250100) marrow transplant globoid cell leukodystrophy GALC (606890) spasticity, optic atrophy, mental retardation; possibly treatable with bone (245200) marrow transplant Refsum Disease (266500) PHYH (602026) deafness,
    [Show full text]
  • (AP-1) Complex at the Heart of Post-Golgi Trafficking in a Corticotrope Tumor Cell Line Mathilde L
    University of Connecticut OpenCommons@UConn Doctoral Dissertations University of Connecticut Graduate School 11-25-2014 The Adaptor Protein 1 (AP-1) Complex at the Heart of Post-Golgi Trafficking in a Corticotrope Tumor Cell Line Mathilde L. Bonnemaison [email protected] Follow this and additional works at: https://opencommons.uconn.edu/dissertations Recommended Citation Bonnemaison, Mathilde L., "The Adaptor Protein 1 (AP-1) Complex at the Heart of Post-Golgi Trafficking in a Corticotrope Tumor Cell Line" (2014). Doctoral Dissertations. 601. https://opencommons.uconn.edu/dissertations/601 The Adaptor Protein 1 Complex (AP-1) at the Heart of Post-Golgi Trafficking in a Corticotrope Tumor Cell Line Mathilde L. Bonnemaison, PhD University of Connecticut, 2014 Peptides act as chemical messengers to regulate physiological functions. First synthesized as inactive precursors, peptides undergo a set of post-translational modifications to gain bioactivity en route through the secretory pathway. Peptides exit the Golgi apparatus in secretory granules (SGs) that are stored until a stimulus triggers their exocytosis. The adaptor protein 1A (AP-1A) is required for SG maturation in neuroendocrine cells and for the formation of specialized SGs such as the glue granules in Drosophila, Weibel-Palade bodies in endothelial cells and rhoptries in Toxoplasma gondii. AP-1A is a heterotetrameric complex (γ/β1/µ1A/σ1) that interacts with cargo proteins and clathrin to transport proteins between the trans-Golgi network (TGN) and endosomes. Lack of any AP-1A subunit is sufficient to impair AP-1A function. A decrease in expression of the µ1A subunit in AtT-20 cells, a corticotrope tumor cell line (sh-µ1A), resulted in vacuolization of the TGN, formation of non-condensing SGs and impaired responsiveness to secretagogue.
    [Show full text]
  • Voltage-Gated Ca2+ Channels and Their Regulation by Alternative Splicing
    Trafficking of N-type voltage-gated Ca2+ channels and their regulation by alternative splicing Natsuko Macabuag Doctor of Philosophy UCL 2015 Department of Neuroscience, Physiology and Pharmacology Division of Biosciences Andrew Huxley Building University College London Gower Street London WC1E 6BT I, Natsuko Macabuag confirm that the work presented in this thesis is my own. Where information is derived from other sources, I confirm that this has been indicated in this thesis. Natsuko Macabuag - 2 - Abstract N-type voltage-gated calcium (CaV2.2) channels are expressed predominantly in the central and peripheral nervous systems and play a crucial role in neurotransmitter release. Expression of these channels at the plasma membrane and in the membrane of presynaptic terminals is key for their function, however, how they are trafficked from the subcellular organelles is still poorly understood. In this study, trafficking of mutually-exclusive alternative splice variants of CaV2.2, containing either exon 37a or 37b at the proximal C- terminus and its mechanisms were examined. CaV2.2 with exon 37a (selectively expressed in nociceptors) reveals a significantly greater intracellular trafficking to the axons and plasma membrane of DRG neurons than CaV2.2 with exon 37b. Further examination of the amino acid sequence in exon 37 uncovers that the canonical binding motifs for adaptor protein 1 (AP-1), YxxΦ and [DE]xxxL[LI], present only in exon 37a are accountable for mediating the enhanced channel trafficking from the trans-Golgi network to the plasma membrane. Finally, the dopamine-2 receptor (D2R) and its agonist-induced activation, reveal differential effects on trafficking of these CaV2.2 isoforms.
    [Show full text]