UBE4B (NM 001105562) Human Tagged ORF Clone Product Data

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for RC226378

UBE4B (NM_001105562) Human Tagged ORF Clone Product data:

Product Type: Expression Plasmids Product Name: UBE4B (NM_001105562) Human Tagged ORF Clone Tag: Myc-DDK Symbol: UBE4B Synonyms: E4; HDNB1; UBOX3; UFD2; UFD2A Vector: pCMV6-Entry (PS100001) E. coli Selection: Kanamycin (25 ug/mL) Cell Selection: Neomycin ORF Nucleotide >RC226378 representing NM_001105562 Sequence: Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC

ATGGAGGAGCTGAGCGCTGATGAGATTCGACGGAGGCGCCTTGCACGACTTGCTGGTGGACAGACCTCTC AGCCAACCACCCCACTCACCTCTCCCCAGAGGGAGAACCCTCCGGGGCCTCCCATAGCGGCATCAGCCCC AGGACCCTCTCAGAGTCTTGGTCTCAATGTCCACAACATGACCCCAGCTACCTCCCCAATAGGTGCATCA GGAGTAGCCCATCGAAGCCAGAGCAGTGAAGGAGTCAGTTCTCTCAGCAGCTCGCCCTCTAATAGCCTTG AAACGCAATCTCAGTCTCTCTCACGTTCCCAGAGCATGGATATCGATGGTGTCTCATGTGAGAAAAGCAT GTCCCAGGTGGATGTGGATTCAGGAATTGAAAACATGGAGGTTGATGAAAATGATCGAAGAGAAAAGCGG AGCCTCAGTGATAAGGAGCCTTCCTCGGGCCCTGAAGTGTCTGAAGAGCAGGCCTTACAGCTGGTCTGTA AGATCTTCCGTGTCTCTTGGAAGGACCGGGACAGAGATGTCATCTTTCTTTCTTCTCTTTCTGCACAGTT TAAGCAGAACCCAAAAGAAGTATTCTCCGATTTTAAGGACTTGATTGGCCAGATTTTAATGGAAGTGCTA ATGATGTCCACTCAGACCAGAGATGAAAACCCATTTGCCAGTCTGACAGCCACATCACAGCCAATTGCTG CAGCAGCACGGTCACCAGACAGAAATCTCTTGCTAAACACTGGCTCCAATCCAGGAACAAGCCCCATGTT CTGCAGCGTGGCTTCCTTTGGTGCCAGCTCTTTGTCTAGCCTCTATGAAAGTAGTCCGGCTCCCACTCCC AGTTTCTGGAGCTCTGTTCCCGTGATGGGCCCGTCTCTTGCCTCACCTTCCCGTGCAGCCAGCCAGTTGG CTGTGCCTTCCACTCCCCTCAGTCCTCACAGTGCAGCCTCTGGAACTGCTGCGGGAAGCCAGCCTTCATC CCCGCGGTATCGCCCCTACACTGTCACTCACCCATGGGCGTCCTCAGGCGTCTCCATTCTGTCGAGCTCC CCAAGTCCCCCTGCCCTCGCCAGTAGCCCCCAAGCAGTGCCCGCCAGCAGTTCCAGACAGAGGCCCAGCA GCACGGGTCCACCCCTACCACCCGCCTCACCCAGTGCCACGAGCAGACGCCCCTCCTCCCTGAGGATCTC TCCTAGTTTGGGAGCCTCTGGTGGAGCAAGTAATTGGGATTCCTACAGTGACCATTTCACCATTGAAACC TGCAAAGAGACAGATATGCTGAACTACCTCATCGAGTGTTTCGACCGAGTTGGAATAGAGGAAAAAAAAG CACCAAAGATGTGCAGCCAGCCAGCAGTCAGCCAGCTTCTGAGCAACATCCGCTCACAGTGCATATCCCA TACTGCTTTAGTACTACAAGGCTCCCTAACACAGCCCAGGTCCTTGCAGCAGCCGTCCTTCCTAGTGCCG TATATGCTGTGTAGGAATCTCCCATATGGCTTCATTCAGGAACTGGTGAGAACCACTCACCAGGATGAAG

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 5 UBE4B (NM_001105562) Human Tagged ORF Clone – RC226378

AAGTGTTCAAGCAGATATTTATCCCCATTTTACAAGGCCTGGCTCTTGCTGCCAAAGAGTGCTCCCTCGA CAGTGACTACTTTAAATACCCCCTCATGGCACTAGGTGAGCTCTGTGAAACCAAGTTTGGGAAGACACAC CCTGTGTGCAATTTGGTTGCTTCTTTGCGGTTGTGGTTGCCGAAATCCTTAAGTCCTGGCTGTGGGCGGG AGCTGCAGAGACTCTCTTACTTAGGGGCTTTCTTTAGCTTCTCAGTCTTTGCAGAAGATGATGTTAAAGT GGTTGAAAAATACTTCTCAGGGCCTGCCATTACCCTGGAAAACACTCGTGTGGTTAGCCAATCATTGCAG CATTACTTAGAGCTCGGAAGGCAAGAGCTTTTTAAGATTCTGCATAGTATTTTGTTAAATGGCGAAACCC GTGAGGCTGCTCTCAGTTACATGGCGGCTGTCGTCAATGCCAATATGAAGAAAGCACAGATGCAGACAGA TGATAGATTGGTGTCTACAGATGGATTTATGCTGAATTTCCTTTGGGTACTGCAGCAGCTAAGTACAAAA ATCAAGTTAGAAACAGTTGATCCCACGTATATTTTTCACCCAAGATGTCGGATTACTCTTCCCAATGATG AGACGCGTGTGAATGCAACGATGGAAGATGTGAATGACTGGCTGACTGAACTCTATGGCGATCAGCCTCC ATTTTCTGAGCCGAAATTCCCTACGGAGTGCTTCTTTCTCACCCTGCATGCTCACCACCTCTCTATTCTG CCTAGTTGCCGTCGCTATATCCGCAGACTCCGGGCTATCCGGGAGCTCAATAGAACTGTAGAAGATTTGA AAAATAATGAAAGCCAATGGAAAGATTCCCCACTGGCAACTAGACACCGCGAAATGCTGAAGCGCTGTAA AACTCAGCTTAAGAAACTGGTACGGTGCAAGGCCTGTGCTGATGCTGGCCTACTTGACGAGAGCTTCCTG AGAAGATGTCTGAATTTTTATGGCCTTCTCATTCAGCTGCTGCTCCGCATCCTGGACCCCGCATATCCCG ATATAACACTGCCTTTAAATTCAGATGTCCCCAAGGTATTTGCAGCGTTGCCTGAGTTTTATGTAGAAGA TGTTGCAGAATTTTTATTTTTTATTGTACAATACTCTCCCCAGGCGCTTTATGAGCCCTGTACTCAGGAT ATTGTGATGTTCCTTGTTGTGATGTTGTGCAACCAGAACTACATCCGAAACCCATATTTGGTGGCCAAAC TGGTAGAAGTCATGTTTATGACCAACCCTGCTGTTCAGCCACGAACCCAGAAGTTTTTTGAAATGATTGA GAACCATCCTCTCTCCACCAAGTTGTTGGTACCTTCCCTGATGAAGTTTTATACAGATGTTGAGCATACC GGAGCCACCAGTGAGTTTTATGACAAGTTCACAATTCGCTATCATATTAGCACCATTTTTAAAAGCCTTT GGCAAAACATAGCTCACCATGGCACCTTTATGGAGGAGTTCAACTCCGGGAAGCAGTTTGTTCGCTATAT AAACATGTTGATAAACGACACGACGTTTTTGCTCGATGAAAGTCTGGAGTCTCTGAAGCGAATCCATGAA GTGCAGGAAGAGATGAAGAACAAAGAACAGTGGGACCAGTTGCCCCGGGATCAGCAGCAGGCTCGTCAGT CTCAGCTTGCTCAGGATGAGCGTGTGTCCCGCTCTTACCTCGCCCTGGCCACCGAAACCGTGGACATGTT CCACATCCTCACGAAGCAGGTCCAGAAGCCCTTCCTCAGACCGGAGCTTGGACCCCGATTGGCTGCAATG CTGAACTTTAATCTTCAGCAACTTTGTGGCCCCAAGTGCCGTGACCTGAAAGTTGAAAACCCTGAGAAAT ACGGCTTTGAACCAAAGAAGCTGTTGGACCAACTGACGGATATTTACTTACAGCTGGACTGTGCTCGGTT CGCGAAAGCCATTGCTGACGACCAGAGATCCTACAGTAAGGAATTGTTTGAAGAAGTTATTTCAAAGATG CGGAAGGCAGGGATCAAATCCACAATAGCAATAGAAAAATTTAAGCTGCTCGCCGAGAAAGTGGAGGAGA TAGTGGCCAAGAACGCACGCGCAGAAATCGACTACAGCGACGCTCCTGATGAGTTCAGAGACCCTCTGAT GGACACCCTCATGACAGACCCCGTGCGGCTGCCCTCTGGCACCATCATGGACCGCTCCATCATCCTGCGG CACCTGCTCAACTCCCCCACGGACCCCTTCAACCGGCAGACGCTGACAGAGAGCATGCTGGAACCAGTGC CAGAACTGAAAGAGCAGATTCAGGCGTGGATGAGAGAGAAACAGAACAGCGATCAC

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 5 UBE4B (NM_001105562) Human Tagged ORF Clone – RC226378

Protein Sequence: >RC226378 representing NM_001105562 Red=Cloning site Green=Tags(s)

MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASAPGPSQSLGLNVHNMTPATSPIGAS GVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKR SLSDKEPSSGPEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVL MMSTQTRDENPFASLTATSQPIAAAARSPDRNLLLNTGSNPGTSPMFCSVASFGASSLSSLYESSPAPTP SFWSSVPVMGPSLASPSRAASQLAVPSTPLSPHSAASGTAAGSQPSSPRYRPYTVTHPWASSGVSILSSS PSPPALASSPQAVPASSSRQRPSSTGPPLPPASPSATSRRPSSLRISPSLGASGGASNWDSYSDHFTIET CKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSLQQPSFLVP YMLCRNLPYGFIQELVRTTHQDEEVFKQIFIPILQGLALAAKECSLDSDYFKYPLMALGELCETKFGKTH PVCNLVASLRLWLPKSLSPGCGRELQRLSYLGAFFSFSVFAEDDVKVVEKYFSGPAITLENTRVVSQSLQ HYLELGRQELFKILHSILLNGETREAALSYMAAVVNANMKKAQMQTDDRLVSTDGFMLNFLWVLQQLSTK IKLETVDPTYIFHPRCRITLPNDETRVNATMEDVNDWLTELYGDQPPFSEPKFPTECFFLTLHAHHLSIL PSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFL RRCLNFYGLLIQLLLRILDPAYPDITLPLNSDVPKVFAALPEFYVEDVAEFLFFIVQYSPQALYEPCTQD IVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPAVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHT GATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHE VQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAM LNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKM RKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILR HLLNSPTDPFNRQTLTESMLEPVPELKEQIQAWMREKQNSDH

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV Restriction Sites: SgfI-RsrII Cloning Scheme:

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 3 / 5 UBE4B (NM_001105562) Human Tagged ORF Clone – RC226378

Plasmid Map:

ACCN: NM_001105562 ORF Size: 3906 bp OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at [email protected] or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. RefSeq: NM_001105562.3 RefSeq ORF: 3909 bp Locus ID: 10277 UniProt ID: O95155 Protein Pathways: Ubiquitin mediated proteolysis MW: 146 kDa

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 4 / 5 UBE4B (NM_001105562) Human Tagged ORF Clone – RC226378

Gene Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]