DOCSLIB.ORG

Parent-Child Play Therapy on Depression Symptoms

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Parent-Child Play Therapy on Depression Symptoms 4 Abstracts - 1st International Nastaran Cancer Symposium-2015 / Journal of Cellular Immunotherapy 1 (2015) 1e45 Parent-child play therapy on depression symptoms in Child depression inventory: It is a self-assessment scale devel- children with cancer decreases perceived stress on their oped by Kovacs (1977), which was designed to assess signs of cogni- mothers and improving parent-child relationship tive, behavioral and emotional depression in children and adolescents a a b aged 7 to 17. In Dehshiri, Najafi, sheikhi and Habibi Askarabad Roya Sadat Alavian , Zahra Tabibi , Abdollah BaniHashem , (2009) test-retest reliability and internal consistency of the question- a,* MohammadSaeid AbdeKhodaee naire respectively was 0/82, 0/83 a b Ferdowsi University of Mashhad, Mashhad, Iran Mashhad Parent-child relationship scale: This scale was developed by University of Medical Sciences, Mashhad, Iran Pianta for the first time in 1994. It contains 33 items that assesses E-mail address: [email protected] parental perception of their relationship with their child. It is applied Extended Abstract for children aged 3-12. This questionnaire was translated by Tahma- sian in 2007 and its validity was assessed by experts. Introduction: Cancer has a difficult therapeutic process. Treat- ment procedures of cancer cause the child be often tired and languish- First the participants answered the PSS, PCRS and CDI question- ing. These children are also more prone to infections and frequently naires as pre-test. Then the individuals in intervention group received e are hospitalized that leads to the separation of children from their 8 sessions of parent child play therapy, at the end, both of interven- families and the community. Pain, fatigue, anxiety, and other symp- tion and control group filled post-test questionnaires. toms are common in those experiencing cancer. Result: Whit regard to depression, the analysis of covariance data Satin, Linden and Philips (2009), in a meta-analysis study found that showed a significant change between pre-test and post-test ¼ < ¼, the risk of death in cancer patients that report depressive symptoms is (F(1,11) 92/34, p 0/001, 0/89). In other words, there is a signif- more than non-depressed patients, The rate of death in patients who icant difference in depression scores between the subjects of experi- show some symptoms of depression is 25%, and in patients with mild mental and control groups at post-test. Post-test scores of Depression or major depression is 39% more than other cancer patients. in the experimental group were significantly lower than the control one. Since childhood cancer is a life-threatening disease, the role of parents, especially mothers as a primary carer, are increasingly high- In the case of perceived stress variable, after controlling the effect lighted and because of changes in the role of parents, parents are fac- of pre-test scores, the efficacy of treatment in post-test scores was not ¼ < ¼, ing with multiple and complex problems. significant (F(1,11) 1/40,p 0/05, 0/11). So, there is no a signifi- cant difference between the subjects of experimental and control Parents often have feelings of depression and anxiety. Patient and groups in mean scores for perceived stress of mothers in the post-test. caregiver characteristics, stressors, stress appraisal, stress coping methods, and social support influence caregivers' quality of life. Stud- For child-parent relationship, there is a significant relationship ies show that mothers of children with cancer report more perceived between pre-test and post-test scores of this variable. The results in stress than mothers of healthy children and even mothers of children the table above shows After controlling effect of pre-test scores, the with Down syndrome. Studies also show that parents of children sur- efficacy of treatment for child-parent relationship in post-test scores ¼ < ¼, vivors of cancer show significantly higher levels of post-traumatic are also significant(F(1,11) 12/84,p 0/01, 0/53). So, there is a stress symptoms compared with healthy children parents. significant difference between the mean scores of parent-child rela- tionship in the post-test in subjects of the experimental and control Play therapy allows children to encounter with emotional needs groups. and doubts, and provide an opportunity to find ways to cope with their hospital circumstances. On the other hand children need sufficient Discussion and conclusion: Childhood cancer is one of the most time to engage emotionally with their parents, and parents should painful diseases for children and families. This illness can threaten know that how interact with their children in an efficient way. The the mental health of the child and his family. Children encounter way in which mothers interact with their children is very important. with painful side effects of cancer medical treatments, such as nausea, Since play therapy is one of the best ways to get into the child's world vomiting, hair loss, fatigue, pain and etc. And because of diseases and it can also have a positive impact on the children's physical and children cannot participate in peers and family communities, their mental health, so if the mothers enter this field, the play can be an life change considerably. Children with cancer are more likely to effective way for mothers to interact with their children. experience symptoms of depression and, compared with other chil- dren, and the risk of death in the children that represent more symp- Material and methods: Participants: Participants were recruited toms of depression is more than other children. from the inpatient oncology unit at Dr. Sheikh Hospital in the city of ’ ’ Mashhad, Iran, over a 5 month period. Patients were included in the Also psychological conditions of the children s parents affect the study if their diagnosis was cancer, they were between 7-12 years old, child behaviors and emotions. Children who have a better relationship their mothers were primary caregiver and were capable of participat- with their parents and their parents experience less stress, have less ing in a 1-hour 8 sessions of play therapy. So 14 children diagnosed behavioral problems and greater adherence to treatment. with cancer and their mothers (28 individuals) entered in final Psychological interventions that aim these problems are great help program. for these children and their parents. One of these interventions is play Measures and procedure: The instruments used to measure therapy that is enjoyable activity for children. On the other side, depression symptoms, perceived stress and Parent-Child Relationship involvement of parents in treatment can lead to better results. were Perceived Stress Scale (PSS), Child depression inventory (CDI), Current study also aims to reduce the symptoms of childhood and Parent-Child Relationship Scale (PCRS). depression, reduce perceived stress on their mothers and improve Perceived stress scale: This scale has three versions with 4, 10 parent - child relationship used parent child play therapy. Parent-child and 14 items respectively. Cohen et al (1983), conducted a study to play therapy involves child and his parent in enjoyable and purposeful evaluate the psychometric properties of all these three versions. Cron- activities, to express emotions and thoughts in a secure atmosphere. bach's alpha of 14-item version was 0/84 and 0/86. In a study carried Training of relaxation techniques to both child and mother and to out by Ghorbani et al (2002) in the Iranian society, Cronbach's alpha instruct empathy and behavior management techniques to mother was 0/81. helps to solve parent and child psychological problems. Intervention Abstracts - 1st International Nastaran Cancer Symposium-2015 / Journal of Cellular Immunotherapy 1 (2015) 1e45 5 that used in current study is effective in reducing symptoms of delivered by adenovirus vectors, is now widespread and other bio- depression and improve parent e child relationship, but it is not logic approaches like the development of oncolytic viruses designed effective on reducing of mothers Perceived stress. to replicate and kill only p53 defective cells. The other way is the development of siRNA and antisense RNA's that activate p53 by Cancer gene therapy to restore P53 function: New way for inhibiting the function of the negative regulators Mdm2, MdmX, an old aim and HPV E6 is effective too. In conclusion Adenovirus-based p53 gene therapy as well as using small molecules include PRIMA that Farzaneh Iravani a, Majid Mojarrad a,b,* can restore the transcriptional function to mutant p53 cell, or NUT- a Department of Medical Genetics, Mashhad University of Medical LIN and RITA which interfere with MDM2-directed p53 degradation, Science, Mashhad, Iran b Medical Genetics Research Center, have been tested in a preclinical stage and some of these approaches Mashhad University of Medical Sciences, Mashhad, Iran are currently in clinical development. E-mail address: [email protected] TOX3 and the risk of breast cancer; an overview Extended Abstract a a a Introduction: The first description of p53 protein is in 1979 and Amir Tajbakhsh , Fahimeh Afzal Javan , Mahdi Rivandi , b,c, since that time there have been more than 35 000 papers published on Alireza Pasdar * a this topic. However even after 30 years after its discovery, it is still Student Research Committee, Department of New Sciences and somewhat of a mystery. The gene encodes transcription factor p53 Technology, Faculty of Medicine, Mashhad University of Medical b is TP53, one of the most commonly altered genes in human cancers. Sciences, Mashhad, IR, Iran Department of New Sciences & P53 protein plays a central role to regulate cell cycle progression, dif- Technology, School of Medicine, Mashhad University of Medical c ferentiation, senescence, DNA repair, and apoptosis. In response of Sciences, Mashhad, Iran Division of Applied Medicine, Medical DNA damage or other stress signals, p53 activity is up-regulated School, University of Aberdeen, Forester Hill, Aberdeen, UK and a series of biological events cascade is initiated that eventually E-mail address: [email protected] results in prevention of tumor development and expansion.
Load more

Recommended publications
  • Screening and Identification of Key Biomarkers in Clear Cell Renal Cell Carcinoma Based on Bioinformatics Analysis
    bioRxiv preprint doi: https://doi.org/10.1101/2020.12.21.423889; this version posted December 23, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Screening and identification of key biomarkers in clear cell renal cell carcinoma based on bioinformatics analysis Basavaraj Vastrad1, Chanabasayya Vastrad*2 , Iranna Kotturshetti 1. Department of Biochemistry, Basaveshwar College of Pharmacy, Gadag, Karnataka 582103, India. 2. Biostatistics and Bioinformatics, Chanabasava Nilaya, Bharthinagar, Dharwad 580001, Karanataka, India. 3. Department of Ayurveda, Rajiv Gandhi Education Society`s Ayurvedic Medical College, Ron, Karnataka 562209, India. * Chanabasayya Vastrad [email protected] Ph: +919480073398 Chanabasava Nilaya, Bharthinagar, Dharwad 580001 , Karanataka, India bioRxiv preprint doi: https://doi.org/10.1101/2020.12.21.423889; this version posted December 23, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Abstract Clear cell renal cell carcinoma (ccRCC) is one of the most common types of malignancy of the urinary system. The pathogenesis and effective diagnosis of ccRCC have become popular topics for research in the previous decade. In the current study, an integrated bioinformatics analysis was performed to identify core genes associated in ccRCC. An expression dataset (GSE105261) was downloaded from the Gene Expression Omnibus database, and included 26 ccRCC and 9 normal kideny samples. Assessment of the microarray dataset led to the recognition of differentially expressed genes (DEGs), which was subsequently used for pathway and gene ontology (GO) enrichment analysis.
    [Show full text]
  • TOX2 Is Differentially Expressed in Non-Small Cell Lung Cancer-PDF 092220
    1 Tox2 is differentially expressed in non-small cell lung cancer and associates with patient 2 survival. 3 Shahan Mamoor1 4 [email protected] East Islip, NY USA 5 6 Non-small cell lung cancer (NSCLC) is the leading cause of cancer death in the United States1. 7 We mined published microarray data2,3,4 to identify differentially expressed genes in NSCLC. 8 We found that the gene encoding the thymyocyte selection-associated high mobility box protein 9 family member 2 transcription factor Tox2 was among the genes whose expression was most quantitatively different in tumors from patients with NSCLC as compared to the lung. Tox2 10 expression was significantly decreased in NSCLC tumors as compared to the lung, and lower 11 expression of Tox2 in patient tumors was significantly associated with worse overall survival. Tox2 may be important for initiation or progression of non-small cell lung cancer in humans. 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Keywords: Tox2, NSCLC, non-small cell lung cancer, systems biology of NSCLC, targeted 27 therapeutics in NSCLC. 28 1 OF 16 1 In 2016, lung cancer resulted in the death of 158,000 Americans; 81% of all patients 2 diagnosed with lung cancer will expire within 5 years5. Non-small cell lung cancer (NSCLC) is 3 4 the most common type of lung cancer, diagnosed in 84% of patients with lung cancer, and 76% 5 of all patients with NSCLC will expire within 5 years5. The rational development of targeted 6 therapeutics to treat patients with NSCLC can be supported by an enhanced understanding of 7 8 fundamental transcriptional features of NSCLC tumors.
    [Show full text]
  • TOX As a Potential Target for Immunotherapy in Lymphocytic Malignancies Chaofeng Liang1,2, Shuxin Huang1, Yujie Zhao1, Shaohua Chen1* and Yangqiu Li1*
    Liang et al. Biomarker Research (2021) 9:20 https://doi.org/10.1186/s40364-021-00275-y REVIEW Open Access TOX as a potential target for immunotherapy in lymphocytic malignancies Chaofeng Liang1,2, Shuxin Huang1, Yujie Zhao1, Shaohua Chen1* and Yangqiu Li1* Abstract TOX (thymocyte selection-associated HMG BOX) is a member of a family of transcriptional factors that contain the highly conserved high mobility group box (HMG-box) region. Increasing studies have shown that TOX is involved in maintaining tumors and promoting T cell exhaustion. In this review, we summarized the biological functions of TOX and its contribution as related to lymphocytic malignancies. We also discussed the potential role of TOX as an immune biomarker and target in immunotherapy for hematological malignancies. Keywords: Thymocyte selection-associated HMG BOX, Biological function, T cell exhaustion, Lymphocytic malignancies, Immune biomarker, Immunotherapy Background located on different chromosomes and have different TOX (thymocyte selection-associated HMG BOX), a functions (Fig. 1, Table 1)[16]. transcription factor, belongs to the high mobility group box (HMG-box) superfamily. In 2002, Wilkinson et al. TOX demonstrated for the first time that TOX plays an im- TOX, also known as KIAA0808, is located on q12.1 of portant role in the differentiation of CD4 + CD8+ human chromosome 8 and has one transcript. The double-positive thymocytes by gene microarray technol- length of the TOX mRNA is 4076 bp, and it contains ogy. Numerous studies have shown that TOX plays a nine exons that translate into a protein consisting of 526 vital role in the development and formation of various amino acids.
    [Show full text]
  • A Computational Approach for Defining a Signature of Β-Cell Golgi Stress in Diabetes Mellitus
    Page 1 of 781 Diabetes A Computational Approach for Defining a Signature of β-Cell Golgi Stress in Diabetes Mellitus Robert N. Bone1,6,7, Olufunmilola Oyebamiji2, Sayali Talware2, Sharmila Selvaraj2, Preethi Krishnan3,6, Farooq Syed1,6,7, Huanmei Wu2, Carmella Evans-Molina 1,3,4,5,6,7,8* Departments of 1Pediatrics, 3Medicine, 4Anatomy, Cell Biology & Physiology, 5Biochemistry & Molecular Biology, the 6Center for Diabetes & Metabolic Diseases, and the 7Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202; 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, Indianapolis, IN, 46202; 8Roudebush VA Medical Center, Indianapolis, IN 46202. *Corresponding Author(s): Carmella Evans-Molina, MD, PhD ([email protected]) Indiana University School of Medicine, 635 Barnhill Drive, MS 2031A, Indianapolis, IN 46202, Telephone: (317) 274-4145, Fax (317) 274-4107 Running Title: Golgi Stress Response in Diabetes Word Count: 4358 Number of Figures: 6 Keywords: Golgi apparatus stress, Islets, β cell, Type 1 diabetes, Type 2 diabetes 1 Diabetes Publish Ahead of Print, published online August 20, 2020 Diabetes Page 2 of 781 ABSTRACT The Golgi apparatus (GA) is an important site of insulin processing and granule maturation, but whether GA organelle dysfunction and GA stress are present in the diabetic β-cell has not been tested. We utilized an informatics-based approach to develop a transcriptional signature of β-cell GA stress using existing RNA sequencing and microarray datasets generated using human islets from donors with diabetes and islets where type 1(T1D) and type 2 diabetes (T2D) had been modeled ex vivo. To narrow our results to GA-specific genes, we applied a filter set of 1,030 genes accepted as GA associated.
    [Show full text]
  • Host-Parasite Interaction of Atlantic Salmon (Salmo Salar) and the Ectoparasite Neoparamoeba Perurans in Amoebic Gill Disease
    ORIGINAL RESEARCH published: 31 May 2021 doi: 10.3389/fimmu.2021.672700 Host-Parasite Interaction of Atlantic salmon (Salmo salar) and the Ectoparasite Neoparamoeba perurans in Amoebic Gill Disease † Natasha A. Botwright 1*, Amin R. Mohamed 1 , Joel Slinger 2, Paula C. Lima 1 and James W. Wynne 3 1 Livestock and Aquaculture, CSIRO Agriculture and Food, St Lucia, QLD, Australia, 2 Livestock and Aquaculture, CSIRO Agriculture and Food, Woorim, QLD, Australia, 3 Livestock and Aquaculture, CSIRO Agriculture and Food, Hobart, TAS, Australia Marine farmed Atlantic salmon (Salmo salar) are susceptible to recurrent amoebic gill disease Edited by: (AGD) caused by the ectoparasite Neoparamoeba perurans over the growout production Samuel A. M. Martin, University of Aberdeen, cycle. The parasite elicits a highly localized response within the gill epithelium resulting in United Kingdom multifocal mucoid patches at the site of parasite attachment. This host-parasite response Reviewed by: drives a complex immune reaction, which remains poorly understood. To generate a model Diego Robledo, for host-parasite interaction during pathogenesis of AGD in Atlantic salmon the local (gill) and University of Edinburgh, United Kingdom systemic transcriptomic response in the host, and the parasite during AGD pathogenesis was Maria K. Dahle, explored. A dual RNA-seq approach together with differential gene expression and system- Norwegian Veterinary Institute (NVI), Norway wide statistical analyses of gene and transcription factor networks was employed. A multi- *Correspondence: tissue transcriptomic data set was generated from the gill (including both lesioned and non- Natasha A. Botwright lesioned tissue), head kidney and spleen tissues naïve and AGD-affected Atlantic salmon [email protected] sourced from an in vivo AGD challenge trial.
    [Show full text]
  • TOX3 Is Expressed in Mammary ER+ Epithelial Cells and Regulates ER
    Seksenyan et al. BMC Cancer (2015) 15:22 DOI 10.1186/s12885-015-1018-2 RESEARCH ARTICLE Open Access TOX3 is expressed in mammary ER+ epithelial cells and regulates ER target genes in luminal breast cancer Akop Seksenyan1, Asha Kadavallore1, Ann E Walts2, Brian de la Torre1, Dror Berel3,4, Samuel P Strom5,6, Parinaz Aliahmad1, Vincent A Funari5 and Jonathan Kaye1,3,7* Abstract Background: A breast cancer susceptibility locus has been mapped to the gene encoding TOX3. Little is known regarding the expression pattern or biological role of TOX3 in breast cancer or in the mammary gland. Here we analyzed TOX3 expression in murine and human mammary glands and in molecular subtypes of breast cancer, and assessed its ability to alter the biology of breast cancer cells. Methods: We used a cell sorting strategy, followed by quantitative real-time PCR, to study TOX3 gene expression in the mouse mammary gland. To study the expression of this nuclear protein in human mammary glands and breast tumors, we generated a rabbit monoclonal antibody specific for human TOX3. In vitro studies were performed on MCF7, BT474 and MDA-MB-231 cell lines to study the effects of TOX3 modulation on gene expression in the context of breast cancer cells. Results: We found TOX3 expression in estrogen receptor-positive mammary epithelial cells, including progenitor cells. A subset of breast tumors also highly expresses TOX3, with poor outcome associated with high expression of TOX3 in luminal B breast cancers. We also demonstrate the ability of TOX3 to alter gene expression in MCF7 luminal breast cancer cells, including cancer relevant genes TFF1 and CXCR4.
    [Show full text]
  • TOX3 Is a Favorable Prognostic Indicator and Potential Immunomodulatory Factor in Lung Adenocarcinoma
    4144 ONCOLOGY LETTERS 18: 4144-4152, 2019 TOX3 is a favorable prognostic indicator and potential immunomodulatory factor in lung adenocarcinoma DE ZENG1, HAOYU LIN2, JIANXIONG CUI2 and WEIQUAN LIANG2 1Department of Medical Oncology, Cancer Hospital of Shantou University Medical College; 2Department of Breast and Thyroid Surgery, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515000, P.R. China Received February 13, 2018; Accepted January 14, 2019 DOI: 10.3892/ol.2019.10748 Abstract. Thymocyte selection-associated high mobility and cytotoxic T lymphocyte-associated protein 4 (CTLA4), as group box (TOX) genes represent a novel family of genes. well as the development of novel immunotherapy agents, lung Deregulated expression of TOXs has been reported in a cancer treatments have improved notably in terms of disease variety of cancer types, including lung cancer. It has also control and survival outcomes (3). However, the success rate been reported that TOXs are crucial regulators of the immune of immunotherapy remains low, and a large number of cancer system. The present study systematically evaluated the prog- types, including lung cancer, remain largely insensitive or nostic values of TOX family members using a set of publicly refractory to PD-1, PD-L1 and CTLA4 blockade (2,4-6). accessible databases, including Oncomine, Kaplan-Meier Therefore, research has focused on identifying novel immu- plotter and cBioPortal. It was revealed that TOX expression notherapy targets or immunomodulatory factors that can profiles differed between lung cancer and normal tissues, be exploited to enhance the response to current immuno- and high expression of TOX mRNAs generally predicted therapeutic agents and address immunotherapy resistance in improved survival outcomes.
    [Show full text]
  • A Dissertation Entitled the Androgen Receptor
    A Dissertation entitled The Androgen Receptor as a Transcriptional Co-activator: Implications in the Growth and Progression of Prostate Cancer By Mesfin Gonit Submitted to the Graduate Faculty as partial fulfillment of the requirements for the PhD Degree in Biomedical science Dr. Manohar Ratnam, Committee Chair Dr. Lirim Shemshedini, Committee Member Dr. Robert Trumbly, Committee Member Dr. Edwin Sanchez, Committee Member Dr. Beata Lecka -Czernik, Committee Member Dr. Patricia R. Komuniecki, Dean College of Graduate Studies The University of Toledo August 2011 Copyright 2011, Mesfin Gonit This document is copyrighted material. Under copyright law, no parts of this document may be reproduced without the expressed permission of the author. An Abstract of The Androgen Receptor as a Transcriptional Co-activator: Implications in the Growth and Progression of Prostate Cancer By Mesfin Gonit As partial fulfillment of the requirements for the PhD Degree in Biomedical science The University of Toledo August 2011 Prostate cancer depends on the androgen receptor (AR) for growth and survival even in the absence of androgen. In the classical models of gene activation by AR, ligand activated AR signals through binding to the androgen response elements (AREs) in the target gene promoter/enhancer. In the present study the role of AREs in the androgen- independent transcriptional signaling was investigated using LP50 cells, derived from parental LNCaP cells through extended passage in vitro. LP50 cells reflected the signature gene overexpression profile of advanced clinical prostate tumors. The growth of LP50 cells was profoundly dependent on nuclear localized AR but was independent of androgen. Nevertheless, in these cells AR was unable to bind to AREs in the absence of androgen.
    [Show full text]
  • Pflugers Final
    CORE Metadata, citation and similar papers at core.ac.uk Provided by Serveur académique lausannois A comprehensive analysis of gene expression profiles in distal parts of the mouse renal tubule. Sylvain Pradervand2, Annie Mercier Zuber1, Gabriel Centeno1, Olivier Bonny1,3,4 and Dmitri Firsov1,4 1 - Department of Pharmacology and Toxicology, University of Lausanne, 1005 Lausanne, Switzerland 2 - DNA Array Facility, University of Lausanne, 1015 Lausanne, Switzerland 3 - Service of Nephrology, Lausanne University Hospital, 1005 Lausanne, Switzerland 4 – these two authors have equally contributed to the study to whom correspondence should be addressed: Dmitri FIRSOV Department of Pharmacology and Toxicology, University of Lausanne, 27 rue du Bugnon, 1005 Lausanne, Switzerland Phone: ++ 41-216925406 Fax: ++ 41-216925355 e-mail: [email protected] and Olivier BONNY Department of Pharmacology and Toxicology, University of Lausanne, 27 rue du Bugnon, 1005 Lausanne, Switzerland Phone: ++ 41-216925417 Fax: ++ 41-216925355 e-mail: [email protected] 1 Abstract The distal parts of the renal tubule play a critical role in maintaining homeostasis of extracellular fluids. In this review, we present an in-depth analysis of microarray-based gene expression profiles available for microdissected mouse distal nephron segments, i.e., the distal convoluted tubule (DCT) and the connecting tubule (CNT), and for the cortical portion of the collecting duct (CCD) (Zuber et al., 2009). Classification of expressed transcripts in 14 major functional gene categories demonstrated that all principal proteins involved in maintaining of salt and water balance are represented by highly abundant transcripts. However, a significant number of transcripts belonging, for instance, to categories of G protein-coupled receptors (GPCR) or serine-threonine kinases exhibit high expression levels but remain unassigned to a specific renal function.
    [Show full text]
  • BRG1 Knockdown Inhibits Proliferation Through Multiple Cellular Pathways in Prostate Cancer Katherine A
    Giles et al. Clin Epigenet (2021) 13:37 https://doi.org/10.1186/s13148-021-01023-7 RESEARCH Open Access BRG1 knockdown inhibits proliferation through multiple cellular pathways in prostate cancer Katherine A. Giles1,2,3, Cathryn M. Gould1, Joanna Achinger‑Kawecka1,4, Scott G. Page2, Georgia R. Kafer2, Samuel Rogers2, Phuc‑Loi Luu1,4, Anthony J. Cesare2, Susan J. Clark1,4† and Phillippa C. Taberlay3*† Abstract Background: BRG1 (encoded by SMARCA4) is a catalytic component of the SWI/SNF chromatin remodelling com‑ plex, with key roles in modulating DNA accessibility. Dysregulation of BRG1 is observed, but functionally uncharacter‑ ised, in a wide range of malignancies. We have probed the functions of BRG1 on a background of prostate cancer to investigate how BRG1 controls gene expression programmes and cancer cell behaviour. Results: Our investigation of SMARCA4 revealed that BRG1 is over‑expressed in the majority of the 486 tumours from The Cancer Genome Atlas prostate cohort, as well as in a complementary panel of 21 prostate cell lines. Next, we utilised a temporal model of BRG1 depletion to investigate the molecular efects on global transcription programmes. Depleting BRG1 had no impact on alternative splicing and conferred only modest efect on global expression. How‑ ever, of the transcriptional changes that occurred, most manifested as down‑regulated expression. Deeper examina‑ tion found the common thread linking down‑regulated genes was involvement in proliferation, including several known to increase prostate cancer proliferation (KLK2, PCAT1 and VAV3). Interestingly, the promoters of genes driving proliferation were bound by BRG1 as well as the transcription factors, AR and FOXA1.
    [Show full text]
  • FOXA1 Overexpression Mediates Endocrine Resistance by Altering The
    FOXA1 overexpression mediates endocrine resistance PNAS PLUS by altering the ER transcriptome and IL-8 expression in ER-positive breast cancer Xiaoyong Fua,b,c, Rinath Jeselsohnd, Resel Pereiraa,b,c, Emporia F. Hollingsworthe, Chad J. Creightonb,f, Fugen Lid, Martin Sheaa,b,f, Agostina Nardonea,b,f, Carmine De Angelisa,b,f, Laura M. Heiserg, Pavana Anurh, Nicholas Wangg, Catherine S. Grassog, Paul T. Spellmanh, Obi L. Griffithi, Anna Tsimelzona,b,f, Carolina Gutierreze, Shixia Huangb,c, Dean P. Edwardsb,c,e, Meghana V. Trivedia,b,j,k, Mothaffar F. Rimawia,b,f, Dolores Lopez-Terradae, Susan G. Hilsenbecka,b,f, Joe W. Grayg, Myles Brownd, C. Kent Osbornea,b,c,f, and Rachel Schiffa,b,c,f,1 aLester and Sue Smith Breast Center, Baylor College of Medicine, Houston, TX 77030; bDan L. Duncan Cancer Center, Baylor College of Medicine, Houston, TX 77030; cDepartment of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030; dDana–Farber Cancer Institute, Harvard Medical School, Boston, MA 02215; eDepartment of Pathology, Baylor College of Medicine, Houston, TX 77030; fDepartment of Medicine, Baylor College of Medicine, Houston, TX 77030; gDepartment of Biomedical Engineering, Oregon Health and Science University, Portland, OR 97239; hDepartment of Molecular and Medical Genetics, Oregon Health and Science University, Portland, OR 97239; iMcDonnell Genome Institute, Washington University, St. Louis, MO 63108; jDepartment of Pharmacy Practice and Translational Research, University of Houston, Houston, TX 77204; and kDepartment of Pharmacological and Pharmaceutical Sciences, University of Houston, Houston, TX 77204 Edited by Bert W. O’Malley, Baylor College of Medicine, Houston, TX, and approved August 26, 2016 (received for review May 18, 2016) Forkhead box protein A1 (FOXA1) is a pioneer factor of estrogen renders these genomic regions more accessible to other tran- receptor α (ER)–chromatin binding and function, yet its aberration scription factors, such as ER (9), progesterone receptor (PR) in endocrine-resistant (Endo-R) breast cancer is unknown.
    [Show full text]
  • Genetic Polymorphisms of Estrogen Receptor Genes Are Associated With
    Dai et al. Cancer Cell Int (2019) 19:11 https://doi.org/10.1186/s12935-019-0727-z Cancer Cell International PRIMARY RESEARCH Open Access Genetic polymorphisms of estrogen receptor genes are associated with breast cancer susceptibility in Chinese women Zhijun Dai1,2*† , Tian Tian1,2†, Meng Wang2†, Tielin Yang3, Hongtao Li4, Shuai Lin2, Qian Hao1,2, Peng Xu1, Yujiao Deng1,2, Linghui Zhou1,2, Na Li1,2 and Yan Diao2* Abstract Background: Estrogen exposure is a widely known risk factor for BC. And the interaction of estrogen with estrogen receptor (ER) plays an important role in breast cancer development. This case–control study aims to assess the association of genetic polymorphisms in the estrogen receptor genes with breast cancer (BC) susceptibility in Chinese Han women. Methods: Four polymorphisms (rs2881766, rs9383951, rs9340799 in ESR1 and rs3020449 in ESR2) were genotyped in 459 patients and 549 healthy controls using the Sequenom MassARRAY method. Odds ratio (OR) and 95% confdence intervals (95% CI) were calculated to evaluate the associations. False-positive report probability (FPRP) was utilized to examine the noteworthiness of signifcant fndings. Results: We observed that rs2881766 was associated with a decreased BC risk (GG vs. TT: OR 0.63, 95% CI 0.44– 0.91; GG vs. TT/GT: OR 0.68, 95% CI 0.49–0.95), while rs3020449 was associated with an increased= risk of BC= (CT vs. TT: OR 1.58, 95% CI = 1.21–2.06; CT/CC= vs. TT: OR 1.54, 95% CI 1.20–1.98; TT/CC vs. CT: OR 1.48, 95% CI 1.15– 1.90).
    [Show full text]