DOCSLIB.ORG

Manual: Cdna Synthesis Kit, ZAP-Cdna Synthesis Kit, and ZAP

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Manual: Cdna Synthesis Kit, ZAP-Cdna Synthesis Kit, and ZAP cDNA Synthesis Kit, ZAP-cDNA Synthesis Kit, and ZAP-cDNA Gigapack III Gold Cloning Kit Instruction Manual Catalog #200400 (ZAP-cDNA Synthesis Kit), #200401 (cDNA Synthesis Kit), and #200450 (ZAP-cDNA Gigapack III Gold Cloning Kit) Revision D Research Use Only. Not for Use in Diagnostic Procedures. 200401-12 LIMITED PRODUCT WARRANTY This warranty limits our liability to replacement of this product. No other warranties of any kind, express or implied, including without limitation, implied warranties of merchantability or fitness for a particular purpose, are provided by Agilent. Agilent shall have no liability for any direct, indirect, consequential, or incidental damages arising out of the use, the results of use, or the inability to use this product. ORDERING INFORMATION AND TECHNICAL SERVICES Email [email protected] World Wide Web www.genomics.agilent.com Telephone Location Telephone United States and Canada 800 227 9770 Austria 01 25125 6800 Benelux 02 404 92 22 Denmark 45 70 13 00 30 Finland 010 802 220 France 0810 446 446 Germany 0800 603 1000 Italy 800 012575 Netherlands 020 547 2600 Spain 901 11 68 90 Sweden 08 506 4 8960 Switzerland 0848 8035 60 UK/Ireland 0845 712 5292 All Other Countries Please visit www.genomics.agilent.com and click Contact Us cDNA Synthesis Kit, ZAP-cDNA Synthesis Kit, and ZAP-cDNA Gigapack III Gold Cloning Kit CONTENTS Materials Provided .............................................................................................................................. 1 Reagents and Labware Provided with the cDNA Synthesis Kit ............................................ 2 Storage Conditions .............................................................................................................................. 3 Additional Materials Required .......................................................................................................... 3 Reagents and Solutions.......................................................................................................... 3 Equipment ............................................................................................................................. 3 Notice to Purchaser ............................................................................................................................. 3 Background .......................................................................................................................................... 4 Introduction ......................................................................................................................................... 4 cDNA Synthesis ................................................................................................................................... 5 General Vector Description ................................................................................................................ 8 Overview of the Uni-ZAP XR Vector System ...................................................................... 8 Uni-ZAP XR Vector Map ..................................................................................................... 8 pBluescript SK(–) Vector Map .............................................................................................. 9 Bacterial Host Strains ....................................................................................................................... 10 Host Strain Genotypes ......................................................................................................... 10 XL1-Blue MRF’ Bacterial Strain Description ..................................................................... 10 Recommended Media .......................................................................................................... 11 Establishing an Agar Plate Bacterial Stock ......................................................................... 11 Preparing a –80°C Bacterial Glycerol Stock ....................................................................... 12 Growth of Cells for Plating Phage ....................................................................................... 12 Determining Background by Color Selection with IPTG and X-gal ................................... 12 Helper Phage ..................................................................................................................................... 13 Storing the Helper Phage ..................................................................................................... 13 Titering the Helper Phage .................................................................................................... 13 Amplifying the Helper Phage .............................................................................................. 14 The ZAP-cDNA Synthesis Protocol ................................................................................................. 15 Protocol Guidelines ............................................................................................................. 15 Synthesizing First-Strand cDNA ......................................................................................... 15 Synthesizing Second-Strand cDNA .................................................................................... 16 Blunting the cDNA Termini ................................................................................................ 17 Ligating the EcoR I Adapters .............................................................................................. 19 Phosphorylating the EcoR I Ends ........................................................................................ 19 Digesting with Xho I ............................................................................................................ 19 Size Fractionating ................................................................................................................ 20 Ligating the cDNA Insert ................................................................................................................. 26 Packaging Reaction ........................................................................................................................... 27 General Information ............................................................................................................ 27 Packaging Instructions ......................................................................................................... 28 Titering the Packaging Reaction ......................................................................................... 29 Testing the Efficiency of the Gigapack III Packaging Extract with the Wild-Type Lambda Control DNA (Optional) .............................................................................................. 30 Determining Background by Blue-White Color Selection ............................................................ 31 Amplifying the Library ..................................................................................................................... 32 Performing Plaque Lifts ................................................................................................................... 33 Hybridizing and Screening ............................................................................................................... 34 Antibody Screening ........................................................................................................................... 35 In Vivo Excision of the pBluescript Phagemid from the Uni-ZAP XR Vector ........................... 35 In Vivo Excision Protocols Using ExAssist Helper Phage with SOLR Strain ............................. 36 Single-Clone Excision Protocol .......................................................................................... 36 Mass Excision Protocol ....................................................................................................... 38 Appendix I: Recovery of Single-Stranded DNA from Cells Containing pBluescript Phagemids40 Single-Stranded Rescue Protocol ........................................................................................ 41 Appendix II: Purifying and Quantifying RNA ............................................................................... 42 Purifying RNA .................................................................................................................... 42 Quantifying RNA ................................................................................................................ 42 Formaldehyde RNA Gel Protocol ....................................................................................... 43 Appendix III: Treating RNA with Methylmercury Hydroxide .................................................... 44 Appendix IV: Alkaline Agarose Gels .............................................................................................. 45 The Slide Technique ............................................................................................................ 45 The Vertical Alkaline Agarose Technique .......................................................................... 46 Conventional Submerged Gels ............................................................................................ 46 Protocol ............................................................................................................................... 47 Appendix V: Ethidium Bromide Plate Assay— Quantitating the cDNA ....................................
Load more

Recommended publications
  • The Genetic Material: DNA the Central Dogma of Genetics
    The Genetic material: DNA The Central Dogma of Genetics DNA transcription Reverse tititranscriptio RNA n translation Protein • A, T, G, C in DNA • A, U, G, C in RNA • DNA is double stranded • DNA has po lar ity 5’ to 3’ • A T base pair, G C base pair • RNA is single stranded, also has polarity , generally referred as upstream and downstream. In RNA: A U base pair G C base pair. This type of base pa iri ng i n RNA causes secon dary st ruct ure. 1. 5’ vs 3’ 2. Purines vs. Pyrimidines 3. A vs. G 4. C vs. T 5. Transitions vs. transversions AT/GC ratios and their applications • Genome composition and chtitiharacterization • Implications in sequencing • Primer design • PCR yields • … The Polarity of DNA Higher order organization of genomes Most chromosomal DNA does not code for proteins or RNAs: e.g., Human genome 3 billion base pairs 25,000 genes x 2,000 bp per gene =5x10= 5 x 107 bp 5 x 107/3 x 109 = 1.67% MlMolecu lar P rob es: The tools of molecular genetics Concept of probes • “For diagnostic tests, the agent that is used to detect the presence of a molecule in the sample”. • “A DNA sequence that is used to detect the presence o f a comp lement ary sequence by hybridization with a nucleic acid sample”. Need for probes • Screen for the gene of interest • Southern blot to understand genomic structure and gene copy numbers • Northern blot for analysis of RNA expression • Verification of allelic amplification in PCR • ..
    [Show full text]
  • 1 Mechanistic Studies of DNA Replication, Lesion Bypass, And
    Mechanistic Studies of DNA Replication, Lesion Bypass, and Editing Dissertation Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the Graduate School of The Ohio State University By Austin Tyler Raper, B.S. The Ohio State University Biochemistry Graduate Program The Ohio State University 2018 Dissertation Committee Dr. Zucai Suo, Advisor Dr. Ross Dalbey Dr. Michael Poirier Dr. Richard Swenson 1 Copyrighted by Austin Tyler Raper 2018 2 Abstract DNA acts as a molecular blueprint for life. Adenosine, cytidine, guanosine, and thymidine nucleotides serve as the building blocks of DNA and can be arranged in near- endless combinations. These unique sequences of DNA may encode genes that when expressed produce RNA, proteins, and enzymes responsible for executing diverse tasks necessary for biological existence. Accordingly, careful maintenance of the molecular integrity of DNA is paramount for the growth, development, and functioning of organisms. However, DNA is damaged upon reaction with pervasive chemicals generated by normal cellular metabolism or encountered through the environment. The resulting DNA lesions act as roadblocks to high-fidelity A- and B-family DNA polymerases responsible for replicating DNA in preparation for cell division which may lead to programmed cell death. Additionally, these lesions may fool the polymerase into making errors during DNA replication, leading to genetic mutations and cancer. Fortunately, the cell has evolved DNA damage tolerance as an emergency response to such lesions. During DNA damage tolerance, a damage-stalled high-fidelity polymerase is substituted for a specialized Y-family polymerase, capable of bypassing the offending DNA lesion, for replication to continue.
    [Show full text]
  • Distinct Co-Evolution Patterns of Genes Associated to DNA Polymerase III Dnae and Polc Stefan Engelen1,2, David Vallenet2, Claudine Médigue2 and Antoine Danchin1,3*
    Engelen et al. BMC Genomics 2012, 13:69 http://www.biomedcentral.com/1471-2164/13/69 RESEARCHARTICLE Open Access Distinct co-evolution patterns of genes associated to DNA polymerase III DnaE and PolC Stefan Engelen1,2, David Vallenet2, Claudine Médigue2 and Antoine Danchin1,3* Abstract Background: Bacterial genomes displaying a strong bias between the leading and the lagging strand of DNA replication encode two DNA polymerases III, DnaE and PolC, rather than a single one. Replication is a highly unsymmetrical process, and the presence of two polymerases is therefore not unexpected. Using comparative genomics, we explored whether other processes have evolved in parallel with each polymerase. Results: Extending previous in silico heuristics for the analysis of gene co-evolution, we analyzed the function of genes clustering with dnaE and polC. Clusters were highly informative. DnaE co-evolves with the ribosome, the transcription machinery, the core of intermediary metabolism enzymes. It is also connected to the energy-saving enzyme necessary for RNA degradation, polynucleotide phosphorylase. Most of the proteins of this co-evolving set belong to the persistent set in bacterial proteomes, that is fairly ubiquitously distributed. In contrast, PolC co- evolves with RNA degradation enzymes that are present only in the A+T-rich Firmicutes clade, suggesting at least two origins for the degradosome. Conclusion: DNA replication involves two machineries, DnaE and PolC. DnaE co-evolves with the core functions of bacterial life. In contrast PolC co-evolves with a set of RNA degradation enzymes that does not derive from the degradosome identified in gamma-Proteobacteria. This suggests that at least two independent RNA degradation pathways existed in the progenote community at the end of the RNA genome world.
    [Show full text]
  • Phenotypic Characterization of Self-Assembling
    PHENOTYPIC CHARACTERIZATION OF SELF-ASSEMBLING PROTEIN FRAGMENTS USING NEGATIVE DOMINANCE A Dissertation by ADRIENNE ELIZABETH ZWEIFEL Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY May 2010 Major Subject: Biochemistry PHENOTYPIC CHARACTERIZATION OF SELF-ASSEMBLING PROTEIN FRAGMENTS USING NEGATIVE DOMINANCE A Dissertation by ADRIENNE ELIZABETH ZWEIFEL Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Approved by: Chair of Committee, James C. Hu Committee Members, Michael Polymenis Donald W. Pettigrew Michael Benedik Head of Department, Gregory D. Reinhart May 2010 Major Subject: Biochemistry iii ABSTRACT Phenotypic Characterization of Self-Assembling Protein Fragments Using Negative Dominance. (May 2010) Adrienne Elizabeth Zweifel, B.S., University of Missouri-Columbia Chair of Advisory Committee: Dr. James C. Hu Protein oligomerization provides a way for cells to modulate function in vivo. In this study, self-assembling protein fragments from ParC, DnaX, and proteins of unknown function were used to generate phenotypes in a dominant negative manner. These fragments were expressed as Thioredoxin (TRX) fusions under the control of the inducible araBAD promoter. Fragments chosen contain only the oligomerization domain of the protein, lacking the regions necessary for catalytic function. Fragments of ParC, a subunit of Topoisomerase (Topo) IV, generated fragment-specific phenotypes. Regions that expressed both the oligomerization domain and CTD of ParC (ParC206-752 and ParC332-752) yielded filamentous cells with several different nucleoid segregation phenotypes. Another ParC fragment containing only the oligomerization domain of ParC (ranging from 333-485) yields a recA-dependent septation defect in a subset of the population.
    [Show full text]
  • A DNA Fragment Containing the Origin of Replication Of
    Proc. Natl. Acad. Sci. USA Vol. 74, No. 7, pp. 2720-2724, July 1977 Biochemistry A DNA fragment containing the origin of replication of the Escherichia coli chromosome (bidirectional replication/dnaA and dnaC initiation mutants/restriction mapping) ROBERT C. MARSH* AND ABRAHAM WORCELt * Biology Program, The University of Texas at Dallas, P.O. Box 688, Richardson, Texas 75080; and t Department of Biochemical Sciences, Princeton University, Princeton, New Jersey 08540 Communicated by Jerard Hurwitz, April 15, 1977 ABSTRACJ A 38 kilobase pair region of the Escherichia coli mg of Na2SO4, and 12.1 g of Tris. The medium additionally K12 chromosome containing the replication origin has been contained 0.2% glucose, 0.4% casamino acids, and 40 ,gg of physically mapped with restriction endonucleases EcoRI and thymine per ml. HindIII. Replication starts within or very near a 1.3 kilobase pair HindIII fragment in the middle of this region and proceeds Radioactive Labeling of Origin Region and Isolation of outward in both directions with apparently equal speed. This DNA. To obtain synchronized initiation of replication, we grew pattern was observed in both dnaA and dnaC temperature- cultures of PC2 and PC5 at 280C to 0.25 OD600 and then shifted sensitive (ts) initiation mutants at the start of the synchronous them to 40°. After 1 hr. the cultures were rapidly cooled to 280 round of replication which occurs after downshift from the and 10-ml aliquots taken at 1- or 2-min intervals for pulse la- nonpermissive to the permissive temperature. beling with 0.4 ml of [3H]thymidine (0.5 mCi/ml and 60 Ci/ The origin of replication of the Escherichia coli chromosome mmol, Schwarz/Mann).
    [Show full text]
  • Thermosensitive DNA Replication (Colony Morphology/Dna Mutations) C
    Proc. Nati. Acad. Sci. USA Vol, 74, No. 8, pp. 3485-3489, August 1977 Genetics Large-scale automated isolation of Escherichia coli mutants with thermosensitive DNA replication (colony morphology/dna mutations) C. G. SEVASTOPOULOS*, C. T. WEHRt, AND D. A. GLASERt Department of Molecular Biology and Virus Laboratory, University of California, Berkeley, Berkeley, California 94720 Contributed by D. A. Glaser, April 25,1977 ABSTRACT We have screened about 1.4 million colonies and study of temperature-sensitive dna mutants (dnat.) are of Escherichia coli K-12 for their ability to grow on nutrient agar known as dnaA, dnaB, dnaC, dnaE, dnaC, dnaH, dnal, dnaP, at 300 and 41°. Among the 2266 temperature-sensitive mutants found, 110 were defective in DNA synthesis but not in protein dnaS, and dnaZ (2-10). (ii) For the biochemists: at least 10 synthesis at 410. Three of these dna mutations mapped at two purified protein fractions have been shown to be required for previously undescribed loci on the E. coli genetic map and may the in tvtro conversion of single-stranded viral 4X174 DNA to represent new genes involved in DNA replication in E coli. The replicative form II (RFII) in an E. coli host; they are dnaB, mutant isolation was aided by novel automatic machinery that dnaC, dnaG, dnaZ proteins, DNA-binding protein, DNA inoculated agar-filled petri dishes with mutagenized K coi cells polymerase III (dnaE protein), DNA elongation factors I and laid down in square arrays of evenly spaced rows and columns on the agar. Time-lapse photographs taken before and after a II, and DNA replication factors X, Y, and Z (11-13).
    [Show full text]
  • A Combined Growth Factor-Deleted and Thymidine Kinase-Deleted Vaccinia Virus Vector
    (19) & (11) EP 2 325 321 A1 (12) EUROPEAN PATENT APPLICATION (43) Date of publication: (51) Int Cl.: 25.05.2011 Bulletin 2011/21 C12N 15/863 (2006.01) A61K 48/00 (2006.01) (21) Application number: 10179286.9 (22) Date of filing: 26.05.2000 (84) Designated Contracting States: • Bartlett, David L. AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU Darnestown, MD 20878 (US) MC NL PT SE • Moss, Bernard Bethesda, MD 20814 (US) (30) Priority: 28.05.1999 US 137126 P (74) Representative: Donald, Jenny Susan (62) Document number(s) of the earlier application(s) in Forrester & Boehmert accordance with Art. 76 EPC: Pettenkoferstrasse 20-22 00939374.5 / 1 180 157 80336 München (DE) (71) Applicant: THE GOVERNMENT OF THE UNITED Remarks: STATES OF AMERICA as •This application was filed on 24-09-2010 as a represented by the SECRETARY OF THE divisional application to the application mentioned DEPARTMENT OF under INID code 62. HEALTH AND HUMAN SERVICES •Claims filed after the date of filing of the application Rockville, MD 20852 (US) / after the date of receipt of the divisional appliaction (Rule 68(4) EPC). (72) Inventors: • McCart, Andrea J. Silver Springs, MD 20910 (US) (54) A combined growth factor-deleted and thymidine kinase-deleted vaccinia virus vector (57) A composition of matter comprising a vaccinia virus expression vector with a negative thymidine kinase phe- notype and a negative vaccinia virus growth factor phenotype. EP 2 325 321 A1 Printed by Jouve, 75001 PARIS (FR) EP 2 325 321 A1 Description Background of the Invention 5 Field of the Invention [0001] The present invention relates to mutant vaccinia virus expression vectors The mutant expression vectors of the present invention show substantially no virus replication in non dividing cells and as such are superior to previous vaccinia virus expression vectors.
    [Show full text]
  • University of Florida Thesis Or Dissertation Formatting
    MECHANISM OF SLIDING CLAMP LOADING BY THE Escherichia coli CLAMP LOADER COMPLEXES By LAUREN GRACE DOUMA A DISSERTATION PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY UNIVERSITY OF FLORIDA 2016 © 2016 Lauren Grace Douma To my family and friends who support me unconditionally ACKNOWLEDGMENTS First, I would like to thank mentors who have helped guide me through graduate school. Most importantly, I need to thank my chair, Dr. Linda Bloom, who constantly encouraged me to think outside of the box and had an enormous amount of patience as I grew as a researcher. I would like to thank my committee members, Dr. Brian Cain, Dr. Kevin Brown, Dr. Sixue Chen, and Dr. Susan Lovett for their invaluable advice throughout my time at the University of Florida. Additionally, I would like to thank Drs. Brain Cain and Kevin Brown for giving me the opportunity to be the teaching assistant for the undergraduate biochemistry course. Lastly, I need to thank my family and friends who have supported me throughout graduate school. The past members of the Bloom laboratory were critical to my success in graduate school. I would like to thank them, not only for always being there to discuss scientific ideas, but also for their friendship. I would like to thank my mother, father, sister for always being available when I needed someone to talk to and encouraging me to follow my dreams. Thank you to my fellow graduate student, Dr. Katie O’Shaughnessey, in addition to the rest of the 2010 cohort, for being a source of laughter, smiles, and encouragement throughout the years.
    [Show full text]
  • The Escherichia Coli DNA Polymerase III Holoenzyme Contains Both Products of the Dnax Gene, T and Y, but Only T Is Essential ALEXANDRA BLINKOVA,1 CHRISTINE HERVAS,1 P
    JOURNAL OF BACTERIOLOGY, Sept. 1993, p. 6018-6027 Vol. 175, No. 18 0021-9193/93/186018-10$02.00/0 Copyright X 1993, American Society for Microbiology The Escherichia coli DNA Polymerase III Holoenzyme Contains Both Products of the dnaX Gene, T and y, but Only T Is Essential ALEXANDRA BLINKOVA,1 CHRISTINE HERVAS,1 P. TODD STUKENBERG,2 RENE ONRUST,2 MICHAEL E. O'DONNELL,2'3 AND JAMES R. WALKER"* Microbiology Department, University of Te-xas, Austin, Texas 78712,1 and Microbiology Department, Cornell University Medical College,2 and Howard Hughes Medical Institute,3 New York, New York 10021 Received 2 April 1993/Accepted 19 July 1993 The replicative polymerase of Escherichia coli, DNA polymerase HI, consists of a three-subunit core polymerase plus seven accessory subunits. Of these seven, r and fy are products of one replication gene, dnaX. The shorter 'y is created from within the 'r reading frame by a programmed ribosomal -1 frameshift over codons 428 and 429 followed by a stop codon in the new frame. Two temperature-sensitive mutations are available in dnaX. The 2016(Ts) mutation altered both 'r and y by changing codon 118 from glycine to aspartate; the 36(Ts) mutation affected the activity only of r because it altered codon 601 (from glutamate to lysine). Evidence which indicates that, of these two proteins, only the longer is essential includes the following. (i) The 36(Ts) mutation is a temperature-sensitive lethal allele, and overproduction of wild-type y cannot restore its growth. (ii) An allele which produced r only could be substituted for the wild-type chromosomal gene, but a -y-only allele could not substitute for the wild-type dnaX in the haploid state.
    [Show full text]
  • Transcriptomic Analysis of Female and Male Gonads in Juvenile Snakeskin
    www.nature.com/scientificreports OPEN Transcriptomic analysis of female and male gonads in juvenile snakeskin gourami (Trichopodus pectoralis) Surintorn Boonanuntanasarn1*, Araya Jangprai1 & Uthairat Na-Nakorn2* The snakeskin gourami (Trichopodus pectoralis) exhibits sexual dimorphism, particularly in body size. Since the snakeskin gourami is usually marketed during sexual maturation, the sexual size dimorphism has become an economically important trait. Sex-biased gene expression plays a key role in phenotypic sexual dimorphism. Therefore, using high-throughput RNA sequencing (RNA-seq) technology, we aimed to explore the diferentially expressed genes (DEGs) in ovary and testis during sex diferentiation in juvenile snakeskin gourami. Our results revealed a number of DEGs were demonstrated to be overexpressed in ovary (11,625 unigenes) and testis (16,120 unigenes), and the top 10 female-biased (rdh7, dnajc25, ap1s3, zp4, polb, parp12, trim39, gucy2g, rtbs, and fdxr) and male-biased (vamp3, nbl1, dnah2, ccdc11, nr2e3, spats1, pih1d2, tekt3, fxo36, and mybl2) DEGs were suggested to be mainly associated with ovary and testis diferentiation, respectively. Additionally, using real-time reverse transcription polymerase chain reaction (qRT-PCR), validation of the diferential expression of 21 genes that were previously shown to be related to gonad development was performed (ar, bHLH, cyp19a1, daz, dead-end, esrb, esrrg, gnrhr, gpa, gsg1l, hsd17B, mospd1, nanos-1, nanos-2, p53, piwi-1, piwi-2, rerg, rps6ka, tgf-beta, and VgR). The results showed a signifcantly positive correlation (0.84; P < 0.001) between the results of RNA-seq and qRT-PCR. Therefore, RNA-seq analysis in our study identifed global genes that were associated with ovary and testis diferentiation in the juvenile phase of the snakeskin gourami.
    [Show full text]
  • Molecular Transport and Structure of Dna in a Congested State
    MOLECULAR TRANSPORT AND STRUCTURE OF DNA IN A CONGESTED STATE ZHU XIAOYING NATIONAL UNIVERSITY OF SINGAPORE 2010 MOLECULAR TRANSPORT AND STRUCTURE OF DNA IN A CONGESTED STATE ZHU XIAOYING (Ph.D.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTER OF PHILOSOPHY DEPARTMENT OF PHYSICS NATIONAL UNIVERSITY OF SINGAPORE 2010 Acknowledgement First and foremost, I would like to thank my supervisor, A/P Johan R. C. van der Maarel for his superb guidance of conducting this research. I appreciate the opportunity for professional and personal growth as a graduate student in one of the top groups in Singapore. General thanks are extended to all former and current members of Johan’s Group for the pervasive spirit of cordial collaboration and the creative atmosphere that produced amazing achievements. Special thanks go to Dai Liang for his selfless and fruitful discussion, Ng Siow Yee and Binu Kundukad for their precious collaborations. Last but not least, acknowledgement must go to my family for their continuous support and sharing, both in storm and sunshine. i List of Publications 1. Viscoelasticity of entangled lambda-phage DNA solutions Xiaoying Zhu, Kundukad Binu, Johan R.C. van der Maarel Journal of Chemical Physics, 129: 185103 (2008) 2. Effect of crowding on the conformation of interwound DNA strands from neutron scattering measurements and Monte Carlo simulations Xiaoying Zhu, Siow Yee Ng, Amar Nath Gupta, Yuan Ping Feng, Bow Ho, Alain Lapp, Stefan U. Egelhaaf, V. Trevor Forsyth, Michael Haertlein, Martine Moulin, Ralf Schweins, and Johan R.C. van
    [Show full text]
  • Modified Recombinant Vaccinia Viruses, Uses Thereof
    (19) & (11) EP 2 028 270 B9 (12) CORRECTED EUROPEAN PATENT SPECIFICATION (15) Correction information: (51) Int Cl.: Corrected version no 1 (W1 B1) C12N 7/04 (2006.01) A61K 35/76 (2006.01) Corrections, see C12N 15/863 (2006.01) A61K 35/74 (2006.01) (2006.01) Description Paragraph(s) 238 C12Q 1/02 (48) Corrigendum issued on: 25.04.2012 Bulletin 2012/17 (45) Date of publication and mention of the grant of the patent: 15.02.2012 Bulletin 2012/07 (21) Application number: 08019998.7 (22) Date of filing: 18.06.2004 (54) Modified recombinant vaccinia viruses, uses thereof Modifizierte, rekombinante Vacciniaviren und deren Verwendungen Virus de vaccinia recombinés modifiés et utilisations associées (84) Designated Contracting States: (74) Representative: Blance, Stephen John AT BE BG CH CY CZ DE DK EE ES FI FR GB GR Boult Wade Tennant HU IE IT LI LU MC NL PL PT RO SE SI SK TR Verulam Gardens Designated Extension States: 70 Gray’s Inn Road AL HR LT LV MK London WC1X 8BT (GB) (30) Priority: 18.06.2003 EP 03013826 (56) References cited: 14.08.2003 EP 03018478 • GNANT M F ET AL: "Systemic administration of 22.10.2003 EP 03024283 a recombinant vaccinia virus expressing the cytosine deaminase gene and subsequent (43) Date of publication of application: treatment with 5-fluorocytosine leads to tumor- 25.02.2009 Bulletin 2009/09 specific gene expression and prolongation of survival in mice" CANCER RESEARCH, (62) Document number(s) of the earlier application(s) in AMERICAN ASSOCIATION FOR CANCER accordance with Art.
    [Show full text]