Recent Publications (Selected from Over 150 Peer-Reviewed Publications)

Recent Publications (selected from over 150 peer-reviewed publications)

1. Li C, Zhu S, Ma C, and Wang LX, “Designer 1,6-Fucosidase Mutants Enable Direct Core Fucosylation of Intact N-Glycopeptides and N-Glycoproteins”, J. Am. Chem. Soc. (2017) available online: http://pubs.acs.org/doi/10.1021/jacs.7b07906

2. Cai H, Orwenyo J, Giddens JP, Yang Y, Zhang,R, LaBranche CC, Montefiori DC, Wang LX, “Synthetic Three-Component HIV-1 V3 Glycopeptide Immunogens Induce Glycan-Dependent Antibody Responses”, Cell Chemical Biology, in press (2017).

3. Yang Q, Wang LX, “Chemoenzymatic Glycan Remodeling of Natural and Recombinant Glycoproteins”, Methods Enzymol., 597, 265-281 (2017).

4. Tong X, Li T, Orwenyo J, Toonstra C, Wang LX, “One-pot enzymatic glycan remodeling of a therapeutic monoclonal antibody by endoglycosidase S (Endo-S) from Streptococcus pyogenes”, Bioorg. Med. Chem., accepted (2017).

5. Yang Q, Zhang R, Cai H, Wang LX. “Revisiting the substrate specificity of mammalian α1,6- fucosyltransferase (FUT8) reveals that it catalyzes core fucosylation of N-glycans lacking α1,3-arm GlcNAc”, J. Biol. Chem., 292, 14796-14803 (2017).

6. Tang F, Wang LX, Huang W. “Chemoenzymatic synthesis of glycoengineered IgG antibodies and glycosite-specific antibody-drug conjugates”, Nature Protocols, 12(8):1702-1721 (2017).

7. Yang M, Huang J, Simon R, Wang LX, MacKerell AD Jr. “Conformational Heterogeneity of the HIV Envelope Glycan Shield”, Scientific Reports, 7(1):4435 (2017).

8. Orwenyo J, Cai H, Giddens J, Amin MN, Toonstra C, Wang LX. “Systematic synthesis and binding study of HIV V3 glycopeptides reveal the fine epitopes of several broadly neutralizing antibodies”, ACS Chem. Biol., 12, 1566-1575 (2017).

9. Cai H, Orwenyo J, Guenaga J, Giddens J, Toonstra C, Wyatt RT, Wang LX, “Synthetic multivalent V3 glycopeptides display enhanced recognition by glycan-dependent HIV-1 broadly neutralizing antibodies”, Chem. Commun. 53, 5453-5456 (2017).

10. Yang Q, An Y, Zhu S, Zhang R, Loke CM, Cipollo JF, Wang LX, “Glycan Remodeling of Human Erythropoietin (EPO) Through Combined Mammalian Cell Engineering and Chemoenzymatic Transglycosylation”, ACS Chem. Biol., 12, 1665-1673 (2017).

11. Feng C, Li J, Snyder G, Huang W, Goldblum SE, Chen WH, Wang LX, McClane BA, Cross AS, “Antibody against Microbial Neuraminidases Recognizes Human Sialidase 3 (NEU3): The Neuraminidase/Sialidase Superfamily Revisited”, MBio, 8, pii: e00078-17 (2017).

12. Li, T., DiLillo, D., Bournazos, S., Giddens, J. P., Ravetch, J. V., Wang, L. X., “Modulating IgG effector functions by Fc glycan engineering”, Proc. Natl. Acad. Sci. USA, 114, 3485-3490 (2017).

13. Freund NT, Wang H, Scharf L, Nogueira L, Horwitz JA, Bar-On Y, Golijanin J, Sievers SA, Sok D, Cai H, Cesar Lorenzi JC, Halper-Stromberg A, Toth I, Piechocka-Trocha A, Gristick HB, van Gils MJ, Sanders RW, Wang LX, Seaman MS, Burton DR, Gazumyan A, Walker BD, West AP Jr, Bjorkman PJ, Nussenzweig MC, “Coexistence of potent HIV-1 broadly neutralizing antibodies and antibody-sensitive viruses in a viremic controller”, Science Translational Medicine, 9, eaal2144 (2017).

14. Yamaguchi, T., Amin, M.N., Toonstra, C., Wang, L.X., “Chemoenzymatic synthesis and receptor binding of mannose-6-phosphate (M6P)-containing glycoprotein ligands reveal unusual structural requirements for M6P receptor recognition”, J. Am. Chem. Soc., 138, 12472-85 (2016).

15. Toonstra, C., Amin, M.N., Wang, L.X., “Site-selective chemoenzymatic glycosylation of an HIV-1 polypeptide antigen with two distinct N-glycans via an orthogonal protecting group strategy”, J. Org. Chem., 81, 6176-6185 (2016).

16. Hsiao, PY, Kalin, JH, Sun, IH, Amin, MN, Lo, YC, Chiang, MJ, Giddens, J, Sysa-Shah, P, Gabrielson, K, Wang, LX, Powell, JD, Cole, PA, “An Fc-small molecule conjugate for targeted inhibition of the adenosine 2A receptor”, ChemBioChem, 17, 1951-1960 (2016).

17. Li, T., Tong, X., Yang, Q., Giddens, J.P., Wang, L.X., “Glycosynthase mutants of endoglycosidase S2 show potent transglycosylation activity and remarkably relaxed substrate specificity for antibody glycosylation remodeling”, J. Biol. Chem., 291, 16508-16518 (2016).

18. Yang, Q., Wang, L.X., “Mammalian α-1,6-fucosyltransferase (FUT8) is the sole enzyme responsible for the N-acetylglucosaminyltransferase I-independent core fucosylation of high-mannose N-glycans”, J. Biol. Chem., 291, 9356-9370 (2016).

19. Giddens, J.P., Lomino, J.V., Amin, M.N., Wang, L.X., “Endo-F3 glycosynthase mutants enable chemoenzymatic synthesis of core fucosylated tri-antennary complex-type glycopeptides and glycoproteins”, J. Biol. Chem., 291, 11064-11071 (2016).

20. Liu, R., Giddens, J., McClung, C.M., Magnelli, P.E., Wang, L.X., Guthrie, E.P., “Evaluation of a glycoengineered monoclonal antibody via LC-MS analysis in combination with multiple enzymatic digestion”, MAbs, 8, 340-6 (2016).

21. Quast, I., Keller, C.W., Maurer, M.A., Giddens, J.P., Tackenberg, B., Wang, L.X., Münz, C., Nimmerjahn, F., Dalakas, M.C., Lünemann, J.D., “Sialylation of IgG Fc domain impairs complement- dependent cytotoxicities”, J. Clin. Invest., 125, 4160-70 (2015).

22. Feng C, Ghosh A, Amin MN, Bachvaroff TR, Tasumi S, Pasek M, Banerjee A, Shridhar S, Wang LX, Bianchet MA, Vasta GR. “Galectin CvGal2 from the Eastern Oyster (Crassostrea virginica) Displays Unique Specificity for ABH Blood Group Oligosaccharides and Differentially Recognizes Sympatric Perkinsus Species”. Biochemistry, 54, 4711-30 (2015).

23. Giddens JP, Wang LX. “Chemoenzymatic Glyco-engineering of Monoclonal Antibodies”. Methods Mol Biol., 1321, 375-87 (2015).

24. Wang D, Tang J, Tang J, Wang LX, “Targeting N-glycan cryptic sugar moieties for broad-spectrum virus neutralization: progress in identifying conserved molecular targets in viruses of distinct phylogenetic origins”. Molecules. 20, 4610-22 (2015).

25. Nita-Lazar M, Banerjee A, Feng C, Amin MN, Frieman MB, Chen WH, Cross AS, Wang LX, Vasta GR. “Desialylation of airway epithelial cells during influenza virus infection enhances pneumococcal adhesion via galectin binding”, Mol Immunol., 65, 1-16 (2015).

26. Ahmed, A.A, Giddens, J.P., Pincetic, A., Lomino, J.V., Ravetch, J.V., Wang, L.X., Bjorkman, P.J., “Structural characterization of anti-inflammatory Immunoglobulin G Fc proteins”, J. Mol. Biol., 426, 3166-79 (2014).

27. Trastoy, B., Lomino, J.V., Pierce, B.G., Carter, L.G., Gunther, S., Giddens, J.P., Snyder, G.A., Weiss, T.M., Weng, Z., Wang, L.X., Sundberg, E.J., “Crystal structure of Streptococcus pyogenes EndoS, an immunomodulatory endoglycosidase specific for human IgG antibodies”, Proc. Natl. Acad. Sci. USA, 111, 6714-6719 (2014).

28. Smith, E.L., Giddens, J.P., Iavarone, A.T., Godula, K., Wang, L.X., Bertozzi, C.R., “Chemoenzymatic Fc glycosylation via engineered aldehyde tags”, Bioconjugate Chem., 25, 788-795 (2014).

29. Chiang, M.J., Holbert, M.A., Kalin, J.H., Ahn, Y.H., Giddens, J, Amin, M.N., Taylor, M.S., Collins, S.L., Chan-Li, Y, Waickman, A, Hsiao, P.Y., Bolduc, D, Leahy, D.J., Horton, M.R., Wang, L.X., Powell, J.D., Cole, P.A., “An Fc domain protein-small molecule conjugate as an enhanced immunomodulator. J. Am. Chem. Soc., 136, 3370-3373 (2014).

30. Wang, L.X., Amin, M.N., “Chemical and chemoenzymatic synthesis of glycoproteins for deciphering functions” (Invited Review), Chemistry & Biology, 21, 51-66 (2014).

31. Lillehoj, E.P., Hyun, S.W., Feng, C., Zhang, L., Liu, A., Guang, W., Nguyen, C., Sun, W., Luzina, I.G., Webb, T.J., Atamas, S.P., Passaniti, A., Twaddell, W.S., Puche, A.C., Wang, L.X., Cross, A.S., Goldblum, S.E., “Human airway epithelia express catalytically active NEU3 sialidase”, Am. J. Physiol. Lung Cell Mol. Physiol., 306, L876-886 (2014).

32. Wang, D., Bhat, R., Sobel, R.A., Huang, W., Wang, L.X., Olsson, T., Steinman, L., Uncovering cryptic glycan markers in multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE). Drug Dev. Res., 75, 172-88 (2014).

33. Heredia, A., Davis, C., Amin, M.N., Le, N.M., Wainberg, M.A., Oliveira, M., Deeks, S.G., Wang, L.X., Redfield, R.R., “Targeting host nucleotide biosynthesis with resveratrol inhibits emtricitabine-resistant HIV-1”, AIDS, 28, 317-23 (2014).

34. Wang, L.X., “Synthetic carbohydrate antigens for HIV vaccine design” (Invited Review), Current Opinion in Chemical Biology, 17, 997–1005 (2013).

35. Wang, L.X., Davis, B.G., “Realizing the promise of chemical glycobiology” (Invited Review), Chemical Science, 4, 3381-3394 (2013).

36. Trastoy, B., Lomino, J.V., Wang, L.X., Sundberg, E.J., “Liquid-liquid diffusion crystallization improves the X-ray diffraction of EndoS, an endo-β-N-acetylglucosaminidase from Streptococcus pyogenes with activity on human IgG”, Acta Crystallogr. Sect F. Struct. Biol. Cryst. Commun., 69, 1405-10 (2013).

37. Amin, M.N., McLellan, J.S., Huang, W., Orwenyo, J., Burton, D.R., Koff, W.C., Kwong, P.D., Wang, L.X., “Synthetic glycopeptides reveal the glycan specificity of HIV-neutralizing antibodies”, Nature Chem. Biol., 9, 521-526 (2013).

38. Pancera, M., Shahzad-ul-Hussan, S., Doria-Rose, N. A., McLellan, J.S., Dai, K., Loesgen, S., Staupe, S.P., Yang, Y., Haynes, B. F., Amin, M.N., Wang, L. X., Burton, D. R., Koff, W.C., Nabel, G.J., Mascola, J.R., Bewley, C.A., Kwong, P.D., “Structural basis for diverse N-glycan recognition and enhanced HIV-1 neutralization by V1/V2-directed antibodies”, Nature Struct. Mol. Biol., 20, 804-813 (2013).

39. Orwenyo, J., Huang, W., Wang, L.X., “Chemoenzymatic synthesis and lectin recognition of a selectively fluorinated glycoprotein”, Bioorg. Med. Chem., 21, 4768-77 (2013).

40. Lomino, J.V., Naegeli, A., Orwenyo, J., Amin, M.N., Aebi, M., Wang, L.X., “A two-step enzymatic glycosylation of polypeptides with complex N-glycans”, Bioorg. Med. Chem., 21, 2262-70 (2013).

41. Wang, H., Huang, W., Orwenyo, J., Banerjee, A., Vasta, G.R., Wang, L.X., “Design and synthesis of glycoprotein-based multivalent glyco-ligands for influenza hemagglutinin and human galectin-3”. Bioorg. Med. Chem., 21, 2037-44 (2013).

42. Heredia, A, Davis, C.E., Reitz, M.S., Le, N.M., Wainberg, M.A., Foulke, J.S., Wang, L.X., Redfield, R.R.,” Targeting of the purine biosynthetic host cell pathway enhances the activity of tenofovir against sensitive drug-resistant HIV-1”, J. Infect. Dis., 208, 2085-94 (2013).

43. Feng, C., Ghosh, A., Amin, M.N., Giomarelli, B., Shridhar, S., Banerjee, A., Fernández-Robledo, J.A., Bianchet, M.A., Wang, L.X., Wilson, I.B., Vasta, G.R., “The galectin CvGal1 from the eastern oyster (Crassostrea virginica) binds to blood group A oligosaccharides on the hemocyte surface”, J. Biol. Chem., 288, 24394-409 (2013).

44. Wang, D., Dafik, L., Nolley, R., Huang, W., Wolfinger, R. D., Wang, L.X., Peehl, D. M., “Anti- oligomannose antibodies as potential serum biomarkers of aggressive prostate cancer”, Drug Develop. Res., 74, 65-80 (2013).

45. Huang, W., Giddens, J., Fan, S.Q., Toonstra, C., Wang, L.X., “Chemoenzymatic glycoengineering of intact IgG antibodies for gain of functions”, J. Am. Chem. Soc., 134, 12308−12318 (2012).

46. Cross, A.S., Hyun, S.W., Miranda-Ribera, A., Feng, C., Liu, A., Nguyen, C., Zhang, L., Luzina, I.G., Atamas, S.P., Twaddell, W.S., Guang, W., Lillehoj, E.P., Puche, A.C., Huang, W., Wang, L.X., Passaniti, A., Goldblum, S.E. “NEU1 and NEU3 sialidase activity expressed in human lung microvascular endothelia. NEU1 restrains endothelial cell migration whereas NEU3 does not”, J. Biol. Chem., 287, 15966-15980 (2012).

47. Fan, S., Huang, W., Wang, L. X. “Remarkable transglycosylation activity of glycosynthase mutants of Endo-D, an endo-β-N-acetylglucosaminidase from Streptococcus pneumoniae”, J. Biol. Chem., 287, 11272–11281 (2012).

48. Lillehoj, E.P., Hyun, S.W., Feng, C., Zhang, L., Liu, A., Guang, W., Nguyen, C., Luzina, I.G., Atamas, S.P., Passaniti, A., Twaddell, W.S., Puché, A.C., Wang, L.X., Cross, A.S., Goldblum, S.E. “NEU1 sialidase expressed in human airway epithelia regulates epidermal growth factor receptor (EGFR) and MUC1 protein signaling”, J. Biol. Chem., 287, 8214-8231 (2012).

49. Song W., Delyria, E.S., Chen, J., Huang, W., Lee, J.S., Mittendorf, E.A., Ibrahim, N., Radvanyi, L.G., Li, Y., Lu, H., Xu, H., Shi, Y., Wang, L.X., Ross, J.A., Rodrigues, S.P., Almeida, I.C., Yang, X., Qu, J., Schocker NS, Michael K, Zhou D., “MUC1 glycopeptide epitopes predicted by computational glycomics”, Int. J. Oncol., 41(6), 1977-84 (2012).

50. Wang, L. X., Lomino, J. V. “Emerging technologies for making glycan-defined glycoproteins” (Invited Review), ACS Chemical Biology, 7, 110-122 (2011).

51. McLellan, J., Pancera, M., Carrico, C., Gorman, J., Julien, J. P., Khayat, R., Louder, R., Pejchal, R., Sastry, M., Dai, K., O’Dell, S., Patel, N., Shahzad-ul-Hussan, S., Yang, Y., Zhang, B., Zhou, T., Zhu, J., Boyington, J. C., Chuang, G. Y., Diwanji, D., Georgiev, I., Kwon, Y. D., Lee, D., Louder, M. K., Moquin, S., Schmidt, S. D., Yang, Z. Y., Bonsignori, M., Crump, J. A., Kapiga, S. H., Sam, N. E., Haynes, B. F., Burton, D. R., Koff, W. C., Walker, L. M., Phogat, S., Wyatt, R., Orwenyo, J., Wang, L. X., Arthos, J., Bewley, C. A., Mascola, J. R., Nabel, G. J., Schief, W. R., Ward, A. B., Wilson, I. A., Kwong, P. D., “Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9”, Nature, 480, 336-343 (2011).

52. Zou, G., Ochiai, H., Huang, W., Yang, Q., Li, C., Wang, L. X., “Chemoenzymatic synthesis and Fc receptor binding of homogeneous glycoforms of antibody Fc domain. Presence of a bisecting sugar moiety enhances the affinity of Fc to FcIIIa receptor”, J. Am. Chem. Soc., 133, 18975-18991 (2011).

53. Amin, M. N., Huang, W., Mizanur, R. M., Wang, L. X., “Convergent synthesis of homogeneous Glc1Man9GlcNAc2-protein and derivatives as ligands of molecular chaperones in protein quality control”, J. Am. Chem. Soc., 133, 14404–14417 (2011).

54. Li, Y., Qiao, H., Xing, C., Zhang, J., Wang, L .X., Wang, H., Zhang, B., Tang, J., “Molecular recognition force spectroscopy of a specific lectin-carbohydrate interaction at single-molecule level”, J. Struct. Biol., 176, 46-51 (2011).

55. Zhou D, Levery S.B., Hsu, FF, Wang PG, Teneberg S, Almeida IC, Li Y, Xu H, Wang LX, Xia C, Ibrahim NK, Michael K, “Immunologic mapping of glycomes: implications for cancer diagnosis and therapy”, Frontier in Bioscience, 3, 1520-1532 (2011).

56. Wang, L. X., “The Amazing Transglycosylation Activity of Endo-beta-N acetylglucosaminidases” (Invited Review), Trends Glycosci. Glycotechnol., 23, 33-52 (2011).

57. Huang, W., Li, J., Wang, L. X., “Unusual transglycosylation activity of Flavobacterium meningosepticum endoglycosidases enables convergent chemoenzymatic synthesis of core fucosylated complex N-glycopeptides”, ChemBioChem, 12, 932-941 (2011).

58. Huang, W., Zhang, X., Ju, T., Cummings, R.D., Wang, L.X. “Expeditious chemoenzymatic synthesis of CD52 glycopeptide antigens”, Org. Biomol. Chem., 8, 5224-5233 (2010).

59. Umekawa, M., Higashiyama, T., Koga, Y., Tanaka, T., Noguchi, M., Kobayashi, A., Shoda, S.I., Huang, W., Wang, L.X., Ashida, H., Yamamoto, K. “Efficient transfer of sialo-oligosaccharide onto proteins by combined use of a glycosynthase-like mutant of Mucor hiemalis endoglycosidase and synthetic sialo-complex-type sugar oxazoline”. Biochim. Biophys. Acta, 1800, 1203-9. (2010).

60. Brooks, C.L., Schietinger, A., Borisova1, S.N., Kufer, P., Okon, M., Hirama, T., MacKenzie, C.R., Wang, L.X., Schreiber, H., Evans, S.V., “Antibody recognition of a novel tumor-specific glycopeptide antigen”, Proc. Natl. Acad. Sci. USA, 107, 10056-10061 (2010).

61. Huang, W., Yang, Q., Umekawa, M., Yamamoto, K., Wang, L.X., “Arthrobacter endo-beta-N- acetylglucosaminidase shows transglycosylation activity on complex type N-glycan oxazolines. One- pot conversion of ribonuclease B to sialylated ribonuclease C”. ChemBioChem, 11, 1350-1355 (2010).

62. Stamatos, N. M., Carubelli, I,. van de Vlekkert, D, Bonten, E.J., Papini, N., Feng, C., Venerando, B., d'Azzo, A., Cross, A.S., Wang, L.X., Gomatos, P.J. “LPS-induced cytokine production in human dendritic cells is regulated by sialidase activity”, J. Leukoc. Biol, 88, 1227-39 (2010).

63. Yang, Q., Li, C., Wei, Y., Huang, W., Wang, L. X., “Expression, glycoform characterization, and antibody-binding of HIV-1 V3 glycopeptide domain fused with human IgG1-Fc”, Bioconjugate Chem., 21, 875-883 (2010).

64. Schwarz, F., Huang, W., Li, C., Schulz, B. L., Lizak, C., Palumbo, A., Numao, S., Neri, D., Aebi, M., Wang, L. X. “A combined method for producing homogeneous glycoproteins with eukaryotic N- glycosylation”, Nature Chem. Biol., 6, 264-266 (2010).

65. Umekawa, M., Li, C., Higashiyama, T., Huang, W., Ashida, H., Yamamoto, K., Wang, L. X., “Efficient

glycosynthase mutant derived from Mucor hiemalis endo--N-acetylglucosaminidase capable of transferring oligosaccharide from both sugar oxazoline and natural N-glycan”, J. Biol. Chem., 281, 511-521 (2010).

66. Huang, W., Wang, D., Yamada, M., Wang, L. X., “Chemoenzymatic synthesis and lectin array characterization of a class of N-glycan clusters”, J. Am. Chem. Soc., 131, 17963-17971 (2009).

67. Huang, W., Li, C., Li, B., Umekawa, M., Yamamoto, K., Zhang, X., Wang, L. X., “Novel glycosynthases enable a highly efficient chemo-enzymatic synthesis of N-glycoproteins carrying intact natural N- glycans”, J. Am. Chem. Soc., 131, 2214-2223 (2009).

68. Zhu, X. Y., Holtz, B., Wang, Y., Wang, L. X., Orndorff, P. E., Guo, A., „Quantitative glycomics from fluidic glycan microarrays“, J. Am. Chem. Soc., 131, 13646-13650 (2009).

69. Wang, L. X., “Expanding the repertoire of glycosynthases”, Chem. Biol., 16, 1026-1027 (2009).

70. Newsom-Davis, T. E., Wang, D., Steinman, L., Chen, P. F. T., Wang, L. X., Simon, A. K., Screaton, G. R., “Enhanced immune recognition of cryptic glycan markers in human tumors”, Cancer Research., 69, 2018-25 (2009).

71. Wang, L. X., Huang, W., “Enzymatic transglycosylation for glycoconjugate synthesis” (Invited Review), Curr. Opin. Chem. Biol., 13, 592–600 (2009).

72. Huang, W., Groothuys S., Heredia A., Kuijpers B.H., Rutjes F.P., van Delft F.L., Wang L.X., “Enzymatic glycosylation of triazole-linked GlcNAc/Glc-peptide: Synthesis, stability, and anti-HIV activity of triazole-linked HIV-1 gp41 glycopeptide C34 analogues”, ChemBioChem, 10, 1234-1242 (2009).

73. Ochiai, H., Huang, W., and Wang, L. X., “Endo-β-N-acetylglucosaminidase catalyzed polymerization of Glc-β-(1→4)-GlcNAc oxazoline: A revisit to the enzymatic transglycosylation”, Carbohydr. Res., 344, 592-8 (2009).

74. Luallen, R. J., Fu, H., Agrawal-Gamse, C., Mboudjeka, I., Huang, W., Lee, F. H., Wang, L. X., Doms, R. W., Geng, Y., “A yeast glycoprotein shows high affinity binding to the broadly neutralizing HIV antibody 2G12 and effectively disrupts gp120 interactions with 2G12 and DC-SIGN”, J. Virol., 83, 4861-70 (2009).

75. Chan, S. L., Voskens, C. J., Lin, W., Schindler, D. G., Azimzadeh, A., Wang, L. X., Taylor, R. J., Strome. S. E., Schulze, D. H., “Epitope mapping of a chimeric CD137 mAb: A necessary step for assessing the biologic relevance of non-human primate models”, J. Mol. Recognition, 22, 242-9 (2009).

76. Yin, J., Li, L., Shaw, N., Li, Y., Song, J. K., Zhang, W., Xia, C., Zhang, R., Joachimiak, A., Wang, L. X., Liu, Z. J., Wang, P. G., “Structural basis and catalytic mechanism for the dual functional endo-beta-N- acetylglucosaminidase A”, PLoS ONE, 4(3):e4658 (2009).

77. Lin, W., Voskens, C.J., Zhang, X., Schindler, D.G., Wood, A., Burch, E., Wei, Y., Chen, L., Tian, G., Tamada, K., Wang, L.X., Schulze, D.H., Mann, D., Strome, S.E., “Fc dependent expression of CD137 on human NK cells: insights into "agonistic" effects of anti-CD137 monoclonal antibodies”, Blood, 112, 699-707 (2008).

78. Huang, W., Ochiai, H., Zhang, X., Wang, L. X., “Introducing N-glycans into natural products through a chemoenzymatic approach”, Carbohydr. Res., 343, 2903-2913 (2008).

79. Li, C., Huang, W., Wang, L. X., “Chemoenzymatic synthesis of N-linked neoglycoproteins through a chitinase-catalyzed transglycosylation”, Bioorg. Med. Chem., 16, 8366-8372 (2008).

80. Li, B., Takegawa, K., Suzuki, T., Yamamoto, K., Wang, L. X., “Synthesis and inhibitory activity of oligosaccharide thiazolines as a class of mechanism-based inhibitors for endo-beta-N- acetylglucosaminidases”, Bioorg. Med. Chem., 16, 4670-4675 (2008).

81. Wang, L. X., “Chemoenzymatic synthesis of glycopeptides and glycoproteins through endoglycosidase-catalyzed transglycosylation”, Carbohydr. Res., 343, 1509-22 (2008).

82. Umekawa, M., Huang, W., Li, B., Fujita, K., Ashida, H., Wang, L. X., Yamamoto, K., “Mutants of Mucor hiemalis endo-beta-N-acetylglucosaminidase show enhanced transglycosylation and glycosynthase- like activities”, J. Biol. Chem., 283, 4469-4479 (2008).

83. Wei, Y., Li, C., Huang, W., Li, B., Strome, S. E., Wang, L. X., “Glyco-engineering of human IgG1-Fc through combined yeast expression and in vitro chemoenzymatic glycosylation”, Biochemistry, 47, 10294-10304 (2008).

84. Ochiai, H., Huang, W., Wei, Y., Wang, L. X., “Expeditious chemoenzymatic synthesis of homogeneous N-glycoproteins carrying defined oligosaccharide ligands”. J. Am. Chem. Soc., 130, 13790-13803 (2008).