Efficient Professional Protein and Antibody Platforms AMY1A Antibody Basic information: Catalog No.: UMA21311 Source: Mouse Size: 50ul/100ul Clonality: Monoclonal 5G6F7 Concentration: 1mg/ml Isotype: Mouse IgG1 Purification: The antibody was purified by immunogen affinity chromatography. Useful Information: WB:1:500 - 1:2000 Applications: FCM:1:200 - 1:400 ELISA:1:10000 Reactivity: Human, Rat Specificity: This antibody recognizes AMY1A protein. Purified recombinant fragment of human AMY1A (AA: 172-284) expressed Immunogen: in E. Coli. are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in di- gestion of dietary starch and glycogen. The has a cluster of Description: several that are expressed at high levels in either salivary gland or pancreas. This encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein. Uniprot: P04745 BiowMW: 57.8kDa Buffer: Purified antibody in PBS with 0.05% sodium azide Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data: Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Figure 2:Western blot analysis using AMY1A mAb against human AMY1A (AA: 172-284) recombi- nant protein. (Expected MW is 38.7 kDa)

Figure 3:Western blot analysis using AMY1A mAb against HEK293 (1) and AMY1A (AA: 172-284)-hIgGFc transfected HEK293 (2) cell ly- sate.

Figure 4:Western blot analysis using AMY1A mouse mAb against A549 (1) and C6 (2) cell ly- sate.

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected]