Valeriana Officinalis (Valerian Root),Echinacea Figure 4.4: Phylogenetic Tree for Ginkgo Biloba Samples D.): N

(—THIS SIDEBAR DOES NOT PRINT—) St. John’s Wort QUICK START (cont.)

DESIGN GUIDE Getting to The Roots of It!

2 2 2 How to change the template color theme This PowerPoint 2007 template produces a 36”x48” Authors: Ahri Han , Yuri Han , and Lauren Kim You can easily change the color theme of your poster by going to the presentation poster. You can use it to create your research 1 DESIGN menu, click on COLORS, and choose the color theme of your Mentor: Dr. Christine Marizzi choice. You can also create your own color theme. poster and save valuable time placing titles, subtitles, text, Echinacea

Valerian Root Ginkgo Biloba 2 and graphics. Teacher: Mrs. Firnberg 1 2 We provide a series of online tutorials that will guide you Cold Spring Harbor Laboratory; Tenafly High School through the poster design process and answer your poster production questions. To view our template tutorials, go online to PosterPresentations.com and click on HELP DESK. St. John’s Wort Samples You can also manually change the color of your background by going to VIEW > SLIDE MASTER. After you finish working on the master be sure to When you are ready to print your poster, go online to This project aimed to use DNA barcoding analysis to go to VIEW > NORMAL to continue working on your poster. PosterPresentations.com identify 4 different herbal supplements found in various Our analysis showed that about 70% of the samples shops throughout New York City: ginkgo biloba, valerian collected were incorrectly labeled. Substitutes are often used How to add Text Figure 1: flow chart of methods Need assistance? Call us at 1.510.649.3001 root, St. John’s wort, and echinacea. By extracting DNA when supply of the ingredient is low or too expensive. Our The template comes with a number of pre- from each sample, the barcode portions of the rbcL gene DNA was extracted from the samples and mixed with formatted placeholders for headers and text results indicate that certain substitutions can be harmful for blocks. You can add more blocks by copying and

were amplified using polymerase chain reaction (PCR). the plant primer specific to the rbcL gene, followed by consumption and raise suspicion on whether or not these pasting the existing ones or by adding a text box The amplified sequences were then sent for sequencing. amplification by PCR. Then gel electrophoresis was used QUICK START labels can be trusted. from the HOME menu. The sequences were used to search the DNA database to confirm the presence of the amplified DNA segments. We identified our species using a primer that targets the using the DNA Subway software to identify closest The DNA was then sent to a commercial lab for Text size Zoom in and out matches. The results that we obtained reflect the possible sequencing. The results were analyzed using DNA rbcL gene, which is a chloroplast gene. Since a portion of Adjust the size of your text based on how much content you have to As you work on your poster zoom in and out to the level that is more species present in their corresponding supplements. Subway software and BLAST algorithm to match our Figure 4.3: Phylogenetic tree for St. John’s Wort samples our samples were roots, which contain little to no present. The default template text offers a good starting point. Follow comfortable to you. the conference requirements. sequenced DNA to species from the Unigene database. Gingko Biloba Samples chloroplasts, it is very likely that we were unable to identify Go to VIEW > ZOOM. the exact species. How to add Tables Title, Authors, and Affiliations New York City holds one of the largest Chinese Regardless, the results obtained from this study bring To add a table from scratch go to the INSERT menu and Start designing your poster by adding the title, the names of the authors, populations in the United States of America. This vast attention to the need of more careful regulations on click on TABLE. A drop-down box will help you select rows and the affiliated institutions. You can type or paste text into the and columns. population characterizes one of many factors that make provided boxes. The template will automatically adjust the size of your mislabeled herbal supplements and the substitutes used in You can also copy and a paste a table from Word or another PowerPoint text to fit the title box. You can manually override this feature and New York City so unique. One influential factor is Chinese their place. document. A pasted table may need to be re-formatted by RIGHT-CLICK > change the size of your text. herbal medicine and its influence within the Chinese FORMAT SHAPE, TEXT BOX, Margins. community and non-Chinese people alike. As herbal TIP: The font size of your title should be bigger than your name(s) and medicines and supplements begin to grow increasingly Graphs / Charts institution name(s). popular in treating numerous ailments, mislabeled products You can simply copy and paste charts and graphs from Excel or Word. Asian American Federation of New York Census Information Center. "Neighborhood Some reformatting may be required depending on how the original are also growing as common problems. The herbs that we Profile: Manhattan’s Chinatown." Neighborhood Profile: Manhattan’s Chinatown (n. document has been created. studied are valeriana officinalis (valerian root),echinacea Figure 4.4: Phylogenetic tree for Ginkgo biloba samples d.): n. pag. Web. .

purpurea (echinacea), hypericum perforatum (St. John’s Valeriana officinalis (Valerian root) matches Bent, Stephen. “Herbal Medicine in the United States: Review of Efficacy, Safety, and How to change the column configuration wort), and ginkgo biloba (ginkgo biloba). Store 1 Store 2 Store 3 Regulation: Grand Rounds at University of California, San Francisco Medical Center.” Adding Logos / Seals Journal of General Internal Medicine 23.6 (2008): 854–859. PMC. Web. 15 Oct. RIGHT-CLICK on the poster background and select LAYOUT to see the Parthenium hysterophorus Valeriana jatamansi Valeriana officinalis Most often, logos are added on each side of the title. You can insert a - Known to cause asthma, dermatitis, - Used for eye problems - Insomnia 2015. column options available for this template. The poster columns can also Figure 2: Electrophoresis gel Results and bronchitis - Heart and Liver - Anxiety disorders - Contain toxins lethal to humans - Hysteria / nervous unrest be customized on the Master. VIEW > MASTER. logo by dragging and dropping it from your desktop, copy and paste or by Name of Uses - Used as flea-repellent - Pain reliever going to INSERT > PICTURES. Logos taken from web sites are likely to be - Used to clear the voice Callaway, Ewen. "Screen Uncovers Hidden Ingredients of Chinese Medicine." Supplement Valeriana capensis Valeriana jatamansi Nature.com. Nature Publishing Group, 12 Apr. 2012. Web. 5 Oct. 2015. . If you are working in PowerPoint for Windows and have finished your

(Valeriana ● Anxiety disorders Valeriana capitata Valeriana capensis "Echinacea: Uses, Side Effects, Interactions and Warnings - WebMD."WebMD. poster, save as PDF and the bars will not be included. You can also delete TIP: See if your school’s logo is available on our free poster templates officinalis) - Remedy for hysteria - Insomnia WebMD, n.d. Web. 15 Oct. 2015. MASTER. On the Mac adjust the Page-Setup to page. KJK-003,-007,-012 - Raw roots are considered poisonous - Oil used to attract rats for traps supplements/ingredientmono-981-echinacea.aspx? match the Page-Setup in PowerPoint before you create a PDF. You can - Epilepsy activeingredientid=981&activeingredientname=echinacea>. also delete them from the Slide Master.

Photographs / Graphics Echinacea ● Common cold Table 2.1: Valerian root matches and effects You can add images by dragging and dropping from your desktop, copy "Ginkgo Biloba (maidenhair Tree)." Ginkgo Biloba. Royal Botanic Gardens, n.d. Web. (Echinacea ● Infections and wounds Figure 3: Ginkgo Biloba (KJK-004) sequence Echinacea purpurea (Echinacea) matches 3 Oct. 2015. . Save your work and paste, or by going to INSERT > PICTURES. Resize images purpurea ) ● Immune system Store 1 Store 2 Store 3 Save your template as a PowerPoint document. For printing, save as proportionally by holding down the SHIFT key and dragging one of the Krüger, Åsa. "DNA-Barcoding identification of medicinal roots from Morocco." (2008). KJK-004, -008, -013 ● Upper respiratory infection Our Samples matches Parthenium hysterophorus Parthenium hysterophorus Parthenium hysterophorus PowerPoint of “Print-quality” PDF. corner handles. For a professional-looking poster, do not distort your - Known to cause asthma, dermatitis, and bronchitis images by enlarging them disproportionally. Valerian Root Samples - Contain toxins lethal to humans O'Connor, Anahad. "Herbal Plus- GNC Distributed Herbal Dietary Supplements." St. John’s Wort ● Depression - Used as flea-repellent New York Attorney General Target Supplements at Major Retailers. New York Times, Print your poster (Hypericum ● Inflammation (minor burns, wounds) Heliopsis helianthoides Heliopsis helianthoides Heliopsis helianthoides 3 Feb. 2015. Web. 3 Oct. 2015. When you are ready to have your poster printed go online to - Invasive species perforatum) ● Premenstrual syndrome . Hypericum perforatum (St. John’s Wort) matches Choose the poster type the best suits your needs and submit your order. If you submit a PowerPoint document you will be receiving a PDF proof Store 1 Store 2 Store 3 Ginkgo Biloba ● Memory (dementia and Alzheimer) Valeriana officinalis Citrus madurensis Hypericum perforatum for your approval prior to printing. If your order is placed and paid for - Insomnia - Calamondin - Depression (Ginkgo biloba) ● Circulatory system - Anxiety disorders - Has Chinese origin - Inflammation (minor burns, wounds) before noon, Pacific, Monday through Friday, your order will ship out that - Premenstrual syndrome KJK-002, -006,-010, ● Eye problems (glaucoma, macular - Menopause same day. Next day, Second day, Third day, and Free Ground services are -011 degeneration) Valeriana jatamansi Citrus hindsii Hypericum punctatum offered. Go to PosterPresentations.com for more information. Image Quality Check - Kumquat - Contains more of the active ingredient, hypericin We thank Mrs. Anat Firnberg, chemistry and science Table 1: lists the names● Sexual of herbal performance supplements problems tested in - improve immune system and related compounds Zoom in and look at your images at 100% magnification. If they look good - regulate digestive system research teacher at Tenafly High School, for her they will print well. Table 1: lists the names and uses of herbal supplements tested in this immeasurable guidance, support, and mentoring for this experiment. Valeriana capitata Hypericum undulatum research project. We would also like to show our gratitude to This Urban Barcoding project aimed to identify herbs Figure 4.1: Phylogenetic tree for Valerian root samples Table 2.3: St. John’s Wort matches and effects Dr. Christine Marizzi, geneticist and educator at Cold Spring Ginkgo biloba (Ginkgo biloba) matches used in traditional Chinese herbal supplements and tea Harbor Laboratory’s DNA Learning Center in Harlem, New with ingredients that are commonly mislabeled or deemed Echinacea Samples Store 1 Store 2 Store 3 Store 4 Maburea trinervis Ginkgo biloba Citrus maxima Citrus maxima York for her precious guidance during the course of this fraudulent in New York City. - Memory (dementia and - Leaves, flowers, and rind: sedative Alzheimer) effect research project. We thank Dr. Marizzi for allowing us to use - Circulatory system - Eye problems (glaucoma, - Compulsive disorders (coughing) macular degeneration) - Peel- skin irritants, may cause the DNA barcoding equipments available at Cold Spring Student discounts are available on our Facebook page. - Sexual performance problems dermatitis from excessive contact with outer peels’ oil Harbor for our research and for her mentoring in our Go to PosterPresentations.com and click on the FB icon. Streptothamnus moorei Citrus reticulata Citrus reticulata - Mandarin orange understanding of DNA barcoding. Berberidopsis beckleri Citrus sinensis Citrus sinensis - Orange Table 2.4: Ginkgo biloba matches and effects Figure 4.2: Phylogenetic tree for Echinacea samples