Apryl E. Pooley CURRICULUM VITAE Program, State University 293 Farm Lane, room 108 East Lansing, MI 48824 [email protected]

EDUCATION PhD in Neuroscience 2011-2017 Michigan State University, East Lansing, MI • Dissertation title: Traumatic stress responses in rats reveal fundamental sex differences that mirror PTSD in men and women

MS in Biological Sciences 2009-2011 Eastern Illinois University, Charleston, IL • Thesis title: Estrogen Promotes Neurite Outgrowth in Olfactory Epithelial Explant Cultures Through the Estrogen Receptor

BS in Biological Sciences 2004-2008 Eastern Illinois University, Charleston, IL • University Honors College Program • Area of concentration: Pre-medicine/

PROFESSIONAL POSITIONS Research Associate, Neuroscience Program 2017-pres Michigan State University, East Lansing, MI • Investigator on neurobiology of PTSD research program Core Faculty, Research Consortium on Gender-Based Violence 2018-pres Michigan State University, East Lansing, MI

EXPERTISE Primary Research Interests • PTSD pathology and neurobiology • How does sex/gender influence the traumatic stress response? • How do early-life developmental processes influence the traumatic stress response? • How does early-life trauma influence psychobiosocial development?

Primary Outreach Interests • Supporting sexual violence prevention/response with evidence-based protocols • Bridging the knowledge gap between trauma researchers and practitioners • Increasing public knowledge of the effects of trauma • Developing institutional and governmental policies that support trauma recovery

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CONTINUING EDUCATION AND TRAINING MI Coalition to End Domestic and Sexual Violence Oct 11, 2017 • Expert Witness Training o Full-day (8hr) training to provide expert testimony in domestic and sexual violence criminal and civil cases

RESEARCH EXPERIENCE Michigan State University August 2017-present Investigator, Cohen Veterans Biosciences grant-funded research

• Project: Do sex differences in gonadal hormones determine sex differences in PTSD?

Michigan State University Jul 2013-Jul 2017 Graduate Student, Cynthia Jordan, PhD and Marc Breedlove, PhD

• Dissertation Project: Sex differences in mechanisms of PTSD • Skills: Single prolonged stress and predator exposure rat models of PTSD, acoustic startle test, rat tail vein blood collection, cFos and glucocorticoid receptor immunohistochemistry, stereology, Western blot, assessments of depressive- and anxiety-like behaviors in rats, gonadal hormone manipulation and replacement, male and female rat gonadectomy

Eastern Illinois University Jan 2009-Jun 2011 Master’s Graduate Student, Britto Nathan, PhD • Thesis Project: Estrogen effects in neurite outgrowth and estrogen receptor localization in olfactory epithelial cultures • Skills: Post-natal and adult brain tissue dissection, neuronal cell culture, immunocytochemical staining, fluorescent microscopy, polymerase chain reaction

TEACHING AND MENTORING Graduate Teaching Assistant Michigan State University • Course: Nervous System Development (NEU 416) Fall 2016

• Course: Psychology and Biology of Human Sexuality (NEU 310) Sp/sum 16, sum 17

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• Course: Introduction to Neuroscience II (NEU 302) Sp 2014, 2015, 2017 o For all above: Lectured on areas of my expertise (brain control of emotion, addiction, PTSD, stress, sexual abuse/assault, ). Held out-of-class review sessions, met one-on-one with students, graded assignments and exams, wrote exam questions. Designed and evaluated a special project for students enrolled to receive honors credit for the course.

Eastern Illinois University • Course: Animal Physiology (BIO 3520) Spring 2011

• Course: General Biology (BIO 1100) Fall 2010

• Course: Endocrinology (BIO 5406, graduate-level class) Spring 2010

• Course: Human Physiology (BIO 2091) Fall 09, Spring 2011

• Course: Genetics (BIO 3200) Fall 09, Spring 2010 o For all above: Organized and supervised the 3-hour laboratory sections of the above courses. Responsibilities included setting up labs, introducing and teaching lab goals and techniques, supervising use of lab equipment, grading lab reports and assignments/exams from the lecture sections of the corresponding course.

Undergraduate Teaching Assistant

Eastern Illinois University • Course: Human Anatomy (BIO 2200) Spring 2008 o Supervised the anatomy lab 6 hours/wk, including helping students identify structures on models and human cadavers

Mentor for Undergraduate Research Michigan State University • Mentee: Susheela Sreedhar, Neuroscience Program Jan 2016-present undergraduate

o Mentored in designing and carrying a project that examined the effects of social housing on the female rat traumatic stress response.

• Mentee: Alla Kedzierski, Neuroscience Program Jan-May 2016 undergraduate o One-semester individual introduction to research with a primary focus on assessing rat behavioral measures of anxiety and depression

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• Mentee: Rebecca Benjamin, Beckman Scholar Program Jun 2014-Jun 2017 and Dean’s Research Scholar undergraduate

o Mentored in designing and carrying out a two-year project that examined the effects of feline predator stress on male and female rats

o Trained student in all techniques and skills used in this project, met one-on-one 4+ times/week to discuss progress, responsible conduct of research, ethics of using animals in stress studies, literature review, grant writing, abstract writing, poster presenting (Rebecca presented 3 research posters based on this work), animal use form writing, experimental design, data interpretation, troubleshooting, critical and creative thinking

• Mentee: Grace Kamau, Neuroscience Program Jan-May 2014 undergraduate

o Mentored in a one-semester individual introduction to research

o Trained student in standard neuroscience research techniques including microtome sectioning, tissue mounting, rat behavior analysis, immunohistochemistry, animal handling, and perfusions. Met one-on-one twice weekly to discuss literature, responsible conduct of research, ethics of using animals in stress studies, experimental design, and data interpretation

HONORS AND AWARDS • MSU Graduate Office Merit Fellowship ($1320) April 2017 • MSU College Nat. Sci. Dissertation Completion F. ($6000) Jan 2017 • MSU College Nat. Sci. Dissertation Continuation F. ($6000) May 2016 • MSU Graduate Office Merit Fellowship ($1250) May 2016 • MSU College Nat. Sci. Dissertation Continuation F. ($6000) May 2015 • MSU Graduate School Travel Award ($500) April 2015 • MSU Neuroscience Program Travel Award ($750) November 2014 • MSU College of Nat. Sci. Dissertation Continuation F. ($6000) May 2014 • MSU Neuroscience Program Travel Award ($750) April 2014 • Eastern Illinois University Honors Program 2004-2008 • Nat’l Science/Math Access to Retain Talent Grant ($4,000) 2006 • Illinois State Scholar 2004 SERVICE AND OUTREACH Service to the Public • Board of Directors, The Firecracker Foundation Jan 2018-present

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Service to Michigan State University • MSU Council of Graduate Students Campus Sexual June 2015-2016

Assault Committee, executive board member o Hold educational events, recommend training for treatment of sexual trauma survivors, create campus-wide syllabus language on options for reporting sexual misconduct and

employee responsibility as mandatory reporters

• Neuroscience Program Graduate Student Council, MSU Graduate Employee Union Steward Mar 2015-2016

o Attend bi-weekly stewards council meetings, keep Neuroscience Program students updated on union and contract issues, participate on contract bargaining team as a negotiator Invited Presentations Lansing/East Lansing Community • Master of Ceremonies, The Firecracker Foundation Nov 12, 2017 o Event: Soulfire 2018, Annual banquet for Lansing community nonprofit serving children who have survived sexual trauma • Master of Ceremonies, The Firecracker Foundation Nov 12, 2016 o Event: Soulfire 2017, Annual banquet for Lansing community nonprofit serving children who have survived sexual trauma • Speaker, Kintla Yoga teacher training in PTSD recovery August 13, 2016 • Master of Ceremonies, The Firecracker Foundation Nov 12, 2015 o Event: Soulfire 2016, Annual banquet for Lansing community nonprofit serving children who have survived sexual trauma

• Speaker/Expert Panelist, Affirmative Consent Town Hall Nov 23, 2015 o Event: State Sen. Curtis Hertel, Jr. and State Rep. Tom

Cochran discuss proposed affirmative consent legislation as a tool to help end sexual assault Michigan State University

• Speaker, “Combating Rape Culture in Academic Settings” March 25, 2016 o Event: MSU 8th Annual Graduate Advocacy Conference • Speaker, MSU Lyman Briggs College senior seminar Sep 16, 2015 o Event: NSF S-STEM scholarship recipient seminar series, “Diversity in Science”

Moderator, roundtable discussion: “Sexual Assault on • Apr 3, 2015 College Campuses: Current Initiatives and Future Challenges” o Event: MSU Center for Gender in Global Context Undergraduate Research Showcase

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• Speaker, MSU Mental Health Awareness Week Apr 1, 2015 o Event: “Embrace the Rain: Stories of Mental Health and Illness,” hosted by MSU Student Health Advisory Council

• Speaker and facilitator, MSU Lyman Briggs College Mar 31, 2015 Women in Science Meeting o Event: Women in Science collaboration with History, Philosophy, and Sociology Students Alliance

• Speaker, “The Human Element in Recovery from Mental Mar 4, 2015 Illness and Addiction” o Event: TEDxMSU, student-organized TED event with 2,000 attendees at the MSU Wharton Center for Performing Arts

Outreach • Brain Awareness Week, Whitehills Elementary School March 17, 2017 demonstration, E. Lansing, MI • Brain Awareness Week, Marble Elementary School March 16, 2017 demonstration, E. Lansing, MI • Booth developer/leader, “Brainatarium” show at MSU March 19, 2016 Neuroscience Fair • Brain Awareness Week, Cornell Elementary School March 16, 2016 demonstration, Okemos, MI • Booth developer/leader, “Brainatarium” show at MSU Women Nov 7, 2015 in Engineering Girl Scout STEM Day • Cast Member, V-Day Lansing presents: “A Memory, A Mar 28, 2015 Monologue, A Rant, A Prayer” fundraiser for the Capital Area Response Effort (Domestic Violence crisis intervention team) • Expert panelist on campus sexual assault policies, MSU Mar 25, 2015 Graduate Employee Union and Union for Non-Tenure Track Faculty “State of Higher Education” Panel Discussion • MSU Representative, American Federation of Teachers Union Mar 24, 2015 Legislative Education Action Day for Higher Education, MI State Capitol • Booth developer/leader, “Brainatarium” show at MSU Mar 21, 2015 Neuroscience Fair • Brain Awareness Week, Cornell Elementary School Mar 18, 2015 demonstration, Okemos, MI • Booth developer/leader, “Brainatarium” show at MSU Mar 7, 2015 Graduate Women in Science Girls Math and Science Day • Regular radio appearances MSU Public Radio (WKAR) and Feb 2015-present Lansing Community College (WLNZ) public radio to discuss campus sexual assault, PTSD, and neuroscience • Organizer and panelist, MSU Graduate Employee Union Town Feb 25, 2015 Hall Meeting for Mandatory Reporting Responsibilities of MSU Employees • Panelist on brain and trauma, MSU Feb 11, 2015 Student Senate Sexual Assault Roundtable • Contributor, The Firecracker Foundation’s “Why I Told” Oct 2014, 2015 storytelling series for survivors of childhood sexual trauma

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• Volunteer Instructor, MSU 4H Exploration Days Jun 2014 • Booth Leader, MSU Neuroscience Fair Mar 2014 • Fundraiser volunteer, River House Domestic violence shelter, Apr 2013 Grayling, MI • Booth volunteer, Brain Awareness Week Neuroscience Fair, Mar 2013 Grand Rapids, MI • Guest, WZZM “Healthy You” news segment, Grand Rapids, MI Mar 2013 • MSU Brain Bee workshop assistant, East Lansing, MI Nov 2012 • Brain Awareness Week, elementary school demonstration, Mar 2012 East Lansing, MI PROFESSIONAL MEMBERSHIPS • Society for Neuroscience 2012-present • Society for Behavioral Neuroendocrinology 2015-present • International Society 2016-present

PUBLICATIONS Pooley, AE (2017). Traumatic stress responses in rats reveal fundamental sex differences that mirror PTSD in men and women. Dissertation. Michigan State University: East Lansing, MI.

Pooley AE, Benjamin RC, Sreedhar S, Eagle AL, Robison AJ, Mazei-Robison MS, Breedlove SM, Jordan CJ (2017) Two traumatic stress models in rats reveal fundamental sex differences that mirror PTSD in men and women. Biological Psychiatry, (submitted).

Pooley, AE, Luong M, Hussain A, Nathan BP (2015) Neurite outgrowth promoting effect of 17-β estradiol is mediated through estrogen receptor alpha in an olfactory epithelium culture. Brain Research, 1624: 19-27.

Hussain A, Luong M, Pooley A, and Nathan BP (2013). Isoform-Specific Effects of ApoE on Neurite Outgrowth in Olfactory Epithelium Culture. J. of Biomedical Science 20:49.

Jia C, Hayoz S, Hutch CR, Iqbal TR, Pooley AE, and Hegg C (2013). An IP3R3- and NPY- Expressing Microvillous Cell Mediates Tissue Homeostasis and Regeneration in the Mouse Olfactory Epithelium. PLoS ONE 8(3):e58668

Pooley, AE (2011). Estrogen Promotes Neurite Outgrowth in Olfactory Epithelial Explant Cultures Through the Estrogen Receptor. Master’s thesis. Eastern Illinois University: Charleston, IL. CONFERENCE PRESENTATIONS

Pooley A.E. Robison, A.J., Mazei-Robison, M.S., Eagle, A.L., Breedlove, S.M., and Jordan, C.L. Sex-specific phenotypes in a rat model of post-traumatic stress disorder (PTSD). Annual Society for Neuroscience Conference. San Diego, CA. November 2016 (Presented Poster)

Pooley A.E., Breedlove S.M., and Jordan, C.L. Sex-specific phenotypes in a rat model of post-traumatic stress disorder (PTSD). 4th Annual MSU Conference on Women’s Health Research. East Lansing, MI. Nov. 2015. (Presented Poster)

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Pooley A.E. Robison, A.J., Mazei-Robison, M.S., Eagle, A.L., Breedlove, S.M., and Jordan, C.L. Sex-Specific Phenotypes in a Rat Model of Post-Traumatic Stress Disorder (PTSD). Annual Society for Behavioral Neuroendocrinology Meeting. Asilomar, CA June 2015 (Presented Poster)

Benjamin, R.C., Pooley A.E., Breedlove, S.M., and Jordan, C.L. Sex-Specific Phenotypes in a Predator Exposure Model of Post-Traumatic Stress Disorder (PTSD). Annual Society for Behavioral Neuroendocrinology Meeting. Asilomar, CA June 2015 (Undergraduate mentee-presented poster)

Pooley A.E. Robison, A.J., Mazei-Robison, M.S., Eagle, A.L., Breedlove, S.M., and Jordan, C.L. The Single Prolonged Stress (SPS) Model of Posttraumatic Stress Disorder (PTSD) Induces a PTSD-like Phenotype in Male Rats but a Depressive-Like Phenotype in Female Rats. Annual Society for Neuroscience Conference. Washington, D.C. November 2014. (Presented Poster)

Pooley A.E., Breedlove S.M., and Jordan, C.L. Sex-specific phenotypes in a rat model of post-traumatic stress disorder (PTSD). 3rd Annual MSU Conference on Women’s Health Research. East Lansing, MI. Oct. 2014. (Presented Poster)

Pooley A.E., Breedlove S.M., and Jordan, C.L. Sex Differences in the Single Prolonged Stress Model of Posttraumatic Stress Disorder. Uniformed Services University Center for the Study of Traumatic Stress Annual Amygdala, Stress, and PTSD Conference. Bethesda, MA. April 2014. (Presented Poster) BOOKS Pooley, Apryl E. (2015). Fortitude: A PTSD Memoir. Seattle, WA: Gravity, 257pp. ISBN: 978- 1-62015-840-1

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