Jorge A. Marchand (787)-529-4634 [email protected] EDUCATION Ph.D The University of California, Berkeley 2013-2019 Department: Chemical and Biomolecular Engineering Thesis Advisor: Prof. Michelle C. Y. Chang Expected Graduation Date: May 2019 B.S. The Johns Hopkins University 2009-2013 Department: Chemical and Biomolecular Engineering Minor: Mathematics Research Advisor: Prof. Denis Wirtz

RESEARCH University of California, Berkeley 2014-2019 Howard Hughes Medical Institute Gilliam Fellow, Chancellor’s Fellow Advisor: Prof. Michelle C. Y. Chang, Department of Chemistry and Molecular & Cell Biology • Discovery of β-ethynylserine (βes) biosynthetic pathway through comparative genomics • Genetic manipulation of Streptomyces cattleya to study βes pathway • Comparative metabolomics studies for elucidation of cryptic enzymatic steps • Metabolic engineering of βes pathway in heterologous host for terminal alkyne amino acid production • Building tools for in vivo incorporation of non-canonical amino acids (propargylglycine, βes) into proteins

The Johns Hopkins University 2010-2013 Undergraduate Research Assistant Advisor: Prof. Denis Wirtz, Department of Chemical and Biomolecular Engineering • Develop high-throughput microscopy methods to characterize cancer cell physical properties • Program image analysis pipeline for feature detection (cell size, shape, motility) • Measure 2D single cell motility as a proxy for metastatic potential

PUBLICATIONS 1. J. A. Marchand, M. E. Neugebauer, M. C. Ing, C. Lin, J. G. Pelton, M. C. Y. Chang, Discovery of a pathway for terminal alkyne amino acid biosynthesis. Nature. (In press) 2. J. A. Marchand, M. E. Neugebauer, M. C. Y. Chang, Terminal alkyne formation in BesB proceeds through a putative terminal allene intermediate. (In preparation) 3. J. A. Marchand, M. Moore, M. E. Neugebauer, M. C. Y. Chang, Activation and control of β-ethynylserine translation. (In preparation) 4. S. B. Khatau, R. J. Bloom, S. Bajpai, D. Razafsky, S. Zang, A. Giri, P.-H. Wu, J. A. Marchand, A. Celedon, C. M. Hale, S. X. Sun, D. Hodzic, and D. Wirtz, “The distinct roles of the nucleus and nucleus-cytoskeleton connections in three-dimensional cell migration.,” Sci. Rep., vol. 2, p. 488, Jan. 2012.

ORAL PRESENTATIONS 1. Howard Hughes Medical Institute Gilliam Fellows Meeting – Biosynthetic pathway for Terminal 2017 Alkyne Amino Acid Biosynthesis – Bethesda, MD 2. Chemical Biology in the Bay Area Day – Biosynthetic pathway for Terminal Alkyne Amino Acid 2017 Biosynthesis – San Francisco, CA 3. Janelia Chemical Tools for Complex Biological Systems Meeting – ‘Flash’ talk: Biosynthetic pathway 2017 for the de novo biosynthesis of terminal alkyne and terminal alkene amino acids – Ashburn, VA 4. American Institute of Chemical Engineers – Annual Meeting: Physical Role of E-Cadherin Mediated 2013 Cell-Cell Adherent Junctions in Multi-cell motility – San Francisco, CA

AWARDS AND HONORS Best Poster – Department of Molecular & Cell Biology Divisional Retreat Spring 2018 Gilliam Fellowship for Advanced Study (Howard Hughes Medical Institute) Fall 2016 Chancellor Fellowship (UC Berkeley) Fall 2013 Fisher Award Fall 2013 NSF-GRFP Honorable Mention Spring 2013 Tau Beta Pi Engineering Honor Society Spring 2013 Golden Key Invited Scholar Fall 2012 GPRDOS Award (Genentech) Fall 2012 Provost Research Undergraduate Award Summer 2011 Dean’s List Fall 2009- Fall 2013

PATENTS 1. PCR Number US2018029229, Michelle C. Y. Chang, Jorge A. Marchand Benmaman, and Monica Neugebauer, “Biosynthesis of bioorthogonal amino acids”, Published 2018-04-25, Assigned to The Regents Of The University Of California.

TEACHING EXPERIENCE The University of California, Berkeley Fall 2015 Graduate Student Instructor – Technical Communication for Chemical Engineers

The University of California, Berkeley Fall 2014 Graduate Student Instructor – Biomolecular Engineering

INDUSTRY EXPERIENCE Genentech – Late Stage Cell Culture Process Engineering Summer 2012 Intern • Late stage development and optimization of a novel αPCSK9 antibody • Scale up antibody production in CHO cells at the 10 L fermentation scale

LEADERSHIP & OUTREACH SACNAS (Society for the Advancement of Chicano/Hispanics and Native Americans in 2016 - 2019 Science – UC Berkeley Graduate Chapter President/Co-chair • Organize events for community STEM outreach, professional development, recruitment • Lead graduate SACNAS chapter manage coordination of student group

ABRCMS (Annual Biomedical Research Conference for Minority Students) Fall 2015 UCB Recruiter • Recruit undergraduate students and encourage them to apply to UC Berkeley graduate programs • Assist administrative staff in providing accurate information

BASIS (Bay Area Scientists in Schools) Fall 2014-2016 Position: Group leader/member • Visit local elementary schools and lead a lesson in science • Plan teaching module and coordinate with classroom teacher

POSTER PRESENTATIONS 1. Howard Hughes Medical Institute Gilliam Fellows 2018 Meeting– Biosynthetic pathway for 2018 terminal alkyne amino acid biosynthesis – Ashburn, VA 2. University of California, Berkeley BBS Retreat – Biosynthetic pathway for terminal alkyne 2018 amino acid biosynthesis – Monterrey, CA 3. Howard Hughes Medical Institute Gilliam Fellows 2017 Meeting– Biosynthetic pathway for 2017 terminal alkyne amino acid biosynthesis – Bethesda, MD 4. Chemical Biology in the Bay Area Day – Biosynthetic pathway for terminal alkyne, alkene, and 2017 halo amino acid biosynthesis – San Francisco, CA 5. Janelia Chemical Tools for Complex Biological Systems Meeting – Biosynthetic pathway for 2017 terminal alkyne amino acid biosynthesis – Ashburn, VA 6. University of California, Berkeley MCB Retreat – De novo biosynthesis of terminal alkyne 2017 amino acids – Monterrey, CA 7. Howard Hughes Medical Institute Gilliam Fellows 2016 Meeting– De novo biosynthesis of 2016 terminal alkyne amino acids – Bethesda, MD REFERENCES 1. Michelle C. Y. Chang – Department of Chemistry and Molecular & Cell Biology – University of California, Berkeley 2. Denis Wirtz – Department of Chemical and Biomolecular Engineering – The Johns Hopkins University 3. David Schaffer – Department of Chemical and Biomolecular Engineering – University of California, Berkeley