Related Polymerases
DNA Polymerase I (E. coli)
Description: Applications: DNA Polymerase I is a multifunctional enzyme that High percentage incorporation of radioactivity for combines a DNA Polymerase activity, a nick translation assays. 5´ 3´exonuclease activity and a 3’ 5’ proofreading Manufacturing of alternating copolymers such exonuclease activity. The 5´ 3´ exonuclease activity as poly d(A-T) and homopolymers such as enables the enzyme to use nicks and gaps in the DNA poly dG-poly dC. as starting points for labelling DNA by nick translation. Klenow fragment: DNA sequencing, fill-in of 5’overhangs and removal of 3’ overhangs to form Advantages & Features: blunt ends and second strand synthesis Native: isolated from E. coli cells with a cloned in mutagenesis. fragment of the polA gene. DNase I-dependent nick translation: Quality control: second-strand synthesis in cDNA cloning, fill-in of Absence of covalently conversion of closed circular Ordering info: 5´ overhangs. DNA to nicked DNA after incubation of 20 units Complete solution: supplied with 10x Reaction of DNA polymerase I with 1 μg of pUC18 DNA for Concentration: 10 U/µL Buffer. 4 hours at 37°C. Cat No. Size P0040 500 U Assays conditions: 67 mM potassium phosphate (pH 7.4), 6.7 mM MgCl2, Includes for 500 U: 1 mM 2-mercaptoethanol, 0.033 mM dATP, · 50 μL DNA Polymerase I (10 U/μL) 0.033 mM dTTP, 0.4 MBq/mL [3H]-dTTP and · 1 mL Reaction Buffer (10x) 62.5 μg/mL poly(dA-dT)·poly(dA-dT).
Related products: · TruePure™ dNTPs (p.115) · DNA Polymerase I, Large (Klenow) Fragment (p.108) · BrightMAX™ DNA Ladders (p.116)
DNA Polymerase I, Large (Klenow) Fragment
Description: Applications: Klenow Fragment is the Large Fragment of DNA DNA sequencing, fill-in of 5’ overhangs and Polymerase I. It shows 5’ 3’ polymerase activity and removal of 3’ overhangs to form blunt ends and 3’ 5’ exonuclease (proofreading) activity, but lacks second strand synthesis in mutagenesis. 5’ 3’ exonuclease activity of DNA Polymerase I. DNA blunting by fill-in of 5’-overhangs or removal of 3‘-overhangs. Advantages & Features: Random-primed DNA labeling. Recombinant: isolated from E. coli cells with a Labeling by fill-in 5’-overhangs of dsDNA. cloned fragment of the polA gene. DNA sequencing by the Sanger method. DNase I-dependent nick translation: Site-specific mutagenesis of DNA with synthetic second-strand synthesis in cDNA cloning, fill-in oligonucleotides. of 5´overhangs. Second strand synthesis of cDNA. Generates probes using random primers. Ordering info: Creates blunt ends. Quality control: Complete solution: supplied with 10x Absence of covalently conversion of closed circular Concentration: 10 U/µL Reaction Buffer. DNA to nicked DNA after incubation of 20 units Cat No. Size of DNA polymerase I with 1 μg of pUC18 DNA for P0041 500 U Unit definition: 4 hours at 37°C. One unit of the enzyme catalyses the incorporation Includes for 500 U: of 10 nmol of deoxyribonucleotides into a · 50 μL DNA Polymerase I (10 U/μL) polynucleotide fraction in 30 min at 37°C, using · 1 mL Reaction Buffer(10x) poly(dA-dT)·poly(dAdT) as a template primer.
Related products: · TruePure™ dNTPs (p.115) · Custom solutions (p.147) · BrightMAX™ DNA Ladders (p.116)
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