Virology Journal BioMed Central Research Open Access The use of RNA-dependent RNA polymerase for the taxonomic assignment of Picorna-like viruses (order Picornavirales) infecting Apis mellifera L. populations Andrea C Baker* and Declan C Schroeder Address: Marine Biological Association, The Laboratory, Citadel Hill, Plymouth, PL1 2PB, UK Email: Andrea C Baker* -
[email protected]; Declan C Schroeder -
[email protected] * Corresponding author Published: 22 January 2008 Received: 19 November 2007 Accepted: 22 January 2008 Virology Journal 2008, 5:10 doi:10.1186/1743-422X-5-10 This article is available from: http://www.virologyj.com/content/5/1/10 © 2008 Baker and Schroeder; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Single-stranded RNA viruses, infectious to the European honeybee, Apis mellifera L. are known to reside at low levels in colonies, with typically no apparent signs of infection observed in the honeybees. Reverse transcription-PCR (RT-PCR) of regions of the RNA-dependent RNA polymerase (RdRp) is often used to diagnose their presence in apiaries and also to classify the type of virus detected. Results: Analysis of RdRp conserved domains was undertaken on members of the newly defined order, the Picornavirales; focusing in particular on the amino acid residues and motifs known to be conserved. Consensus sequences were compiled using partial and complete honeybee virus sequences published to date.