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DNAJC3 Antibody (Center) Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP22323c
Specification
DNAJC3 Antibody (Center) - Product Information
Application WB, FC,E Primary Accession Q13217 Other Accession Q9R0T3 Reactivity Human, Mouse Predicted Rat Host Rabbit Clonality polyclonal Isotype Rabbit IgG
DNAJC3 Antibody (Center) - Additional Information
Gene ID 5611
Other Names DnaJ homolog subfamily C member 3, Anti-DNAJC3 Antibody (Center) at 1:2000 Endoplasmic reticulum DNA J dilution + NIH/3T3 whole cell lysate domain-containing protein 6, ER-resident Lysates/proteins at 20 µg per lane. protein ERdj6, ERdj6, Interferon-induced, Secondary Goat Anti-Rabbit IgG, (H+L), double-stranded RNA-activated protein Peroxidase conjugated at 1/10000 dilution. kinase inhibitor, Protein kinase inhibitor of Predicted band size : 58 kDa 58 kDa, Protein kinase inhibitor p58, DNAJC3, P58IPK, PRKRI Blocking/Dilution buffer: 5% NFDM/TBST.
Target/Specificity This DNAJC3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 189-223 amino acids of human DNAJC3.
Dilution WB~~1:2000 FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity Overlay histogram showing A431 cells purification. stained with AP22323c(green line). The cells were fixed with 2% paraformaldehyde and Storage then permeabilized with 90% methanol for 10 Maintain refrigerated at 2-8°C for up to 2 min. The cells were then icubated in 2% weeks. For long term storage store at -20°C bovine serum albumin to block non-specific in small aliquots to prevent freeze-thaw protein-protein interactions followed by the
Page 1/2 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609
cycles. antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Precautions Goat-Anti-Rabbit IgG, DyLight® 488 DNAJC3 Antibody (Center) is for research Conjugated Highly Cross-Adsorbed at 1/200 use only and not for use in diagnostic or dilution for 40 min at Room temperature. therapeutic procedures. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 DNAJC3 Antibody (Center) - Protein Information events was performed.
Name DNAJC3 DNAJC3 Antibody (Center) - Background Synonyms P58IPK, PRKRI Involved in the unfolded protein response Function (UPR) during ER stress. Co-chaperone of Involved in the unfolded protein response HSPA8/HSC70, it stimulates its ATPase activity. (UPR) during endoplasmic reticulum (ER) May inhibit both the autophosphorylation of stress. Acts as a negative regulator of the EIF2AK2/PKR and the ability of EIF2AK2 to EIF2AK4/GCN2 kinase activity by preventing catalyze phosphorylation of the EIF2A. May the phosphorylation of eIF- 2-alpha at inhibit EIF2AK3/PERK activity. 'Ser-52' and hence attenuating general protein synthesis under ER stress, DNAJC3 Antibody (Center) - References hypothermic and amino acid starving stress conditions (By similarity). Co-chaperone of Korth M.J.,et al.Gene 170:181-188(1996). HSPA8/HSC70, it stimulates its ATPase Dunham A.,et al.Nature 428:522-528(2004). activity. May inhibit both the Polyak S.J.,et al.J. Biol. Chem. autophosphorylation of EIF2AK2/PKR and 271:1702-1707(1996). the ability of EIF2AK2 to catalyze Gale M.J. Jr.,et al.Mol. Cell. Biol. phosphorylation of the EIF2A. May inhibit 18:859-871(1998). EIF2AK3/PERK activity. Melville M.W.,et al.J. Biol. Chem. 274:3797-3803(1999). Cellular Location Endoplasmic reticulum.
Tissue Location Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels
DNAJC3 Antibody (Center) - Protocols
Provided below are standard protocols that you may find useful for product applications.
• Western Blot • Blocking Peptides • Dot Blot • Immunohistochemistry • Immunofluorescence • Immunoprecipitation • Flow Cytomety • Cell Culture
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