J Biodivers Syst 01(1): 17–32 ISSN: 2423-8112

JOURNAL OF INSECT BIODIVERSITY AND SYSTEMATICS

Research Article http://jibs.modares.ac.ir http://zoobank.org/References/9EC96A6C-382D-4472-B77E-42C7A4CD0377

Study of the genus Costa (: : ) in northern , with the description of a new species

Mohammad Khayrandish1, Ali Asghar Talebi1, Stephan M. Blank2 & Yaghoub Fathipour1

1 Deptartement of Entomology, Faculty of Agriculture, Tarbiat Modares University, P O. Box: 14115-336, , Iran 2 Senckenberg Deutshes Entomologisches Institut, Eberswalder Str. 90, 15374 Müncheberg, Germany

Received: ABSTRACT. The genus Ametastegia Costa 1882 (Hymenoptera: 02 September 2015 Tenthredinidae: Allantinae) was studied in northern Iran. The specimens were collected using sweeping net and Malaise traps during March to November Accepted: 05 November 2015 2010 and 2011. Four species were collected and identified of which Ametastegia persica Khayrandish, Talebi & Blank sp. n. is described as new to science. An Published: illustrated key for identification of Ametastegia species in Iran is provided. 15 November 2015

Subject Editor: Ebrahim Ebrahimi Key words: Symphyta, new species, identification key,

Citation: Khayrandish, M., Talebi, A.A., Blank, S.M. and Fathipour, Y. 2015. Study on the genus Ametastegia Costa (Hymenoptera: Tenthredinidae: Allantinae) in northern Iran, with the description of a new species. Journal of Insect Biodiversity and Systematics, 1(1): 17–32.

Introduction 10 species in the West Palaearctic region (Taeger et al. 2010). The subfamily Allantinae (Hymenoptera: (Fallén, 1808) is widely distributed Tenthredinidae) comprising 804 species in throughout the Northern Hemisphere, 107 genera (Taeger et al. 2010). This including temperate Europe, the subfamily has been divided into a number Mediterranean region, Siberia, North of tribes, but different authors used different America, and it has also been recorded classification (Smith 1979; Lacourt 1999; from Chile and eastern Australia Wei and Nie 1998). Most authors believed (Naumann et al. 2002). Some species of that the Allantinae divided into six tribes: Ametastegia, particularly A. glabrata, are Acidiophorini, Allantini, Belesesini, important pests of apple, Malus domestica in Empriini, Eriocampini and Xenapateini. Ukraine, Russia (Storozhenko and The Allantinae is the most diverse Kuznetzov 1995) and USA (CABI 2007). subfamily containing the highest number Damage attributed to the larvae of A. tenera of genera in the family Tenthredinidae (Fallén, 1808), A. glabrata and A. equiseti (Taeger et al. 2010). Genus Ametastegia (Fallén, 1808), has also been reported on Costa, 1882 belongs to the tribe Empriini grapevine, Vitis vinifera in France (Chevin which includes 55 species worldwide and 1979). The first Australian record of A. Corresponding author: Ali Asghar Talebi, E-mail: [email protected] Copyright © 2015, Khayrandish et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 18 Study of the genus Ametastegia in Iran glabrata attacking raspberries, Rubus idaeus present study is an initial attempt to clarify and grapevines has been reported by the taxonomy of the genus Ametastegia and Malipatil et al. (1995). Larvae of most contribution to the knowledge of species of this genus primarily feed on (Hymenoptera: Symphyta) in northern herbaceous hosts from several families of Iran. plants such as Salicaceae, Fagaceae, Polygonaceae, Plantaginaceae, Geraniaceae Materials and Methods and Asteraceae (Taeger et al. 1998; Lacourt North region of Iran is characterized by 1999). great variation in plant community Species of Ametastegia can be composition due to significant differences differentiated from other genera of in topography and climatic changes. In Empriini by the following combination of terms of biodiversity, this region remarked characters: the anal cross vein of the as the end part extensions of two major forewing is perpendicular and not as biodiversity hotspots. Southern slops of oblique as in other genera of Empriini; Alborz Mountains including Tehran, absence of cells Rs and M in the hind wing; Alborz and Qazvin provinces (Fig. 1) shallowly emarginated clypeus and situated in the Irano-Anatolian hotspot, bidentate mandibles. However, some males whereas the northern one, where Gilan and can be readily separated by distinct Mazandaran provinces located, is the characters in the genitalia but there are a eastern extension of the Caucasus few or no reliable diagnostic characters for biodiversity hotspot (Myers et al. 2000). separation in many species. Consequently, The specimens were collected by most species are described based on the sweeping net and Malaise traps during females. Most species have several March to November in 2010 and 2011. generations per year. Mature larvae bore Thirty Malaise traps were installed at into special substrates such as apples or various locations and altitudes in Gilan, other fruits, berry canes and bark and Tehran, Alborz, Qazvin and Mazandaran construct a pupal cell. Such records do not provinces for a period of eight months. The constitute the true host on which the larva collection bottles were filled with 70% actually feeds. Larvae found in these ethanol. Samples were collected weekly or secondary hosts are always prepupae, a biweekly and collecting bottles were stage that cannot be identified properly replaced with fresh ones. All specimens (Smith 1979). were collected from Gilan and Mazandaran According to the recent electronic world provinces in northern slope of Alborz catalogue of Symphyta (Taeger and Blank Mountains (Figs. 2–5). 2011) and other literatures (Zirngiebl 1956; The localities, habitats, sampling dates Benson 1968; Lacourt 1999) only two and geographic coordinates for each species of the genus Ametastegia, A. glabrata species was recorded. For preparation of and A. pallipes have been previously genitalia, the abdomen of specimens were recorded from Iran. dissected and macerated in 10% KOH for Three species of Ametastegia (e.g. A. 12–20 hours at room temperature and then pallipes, A. alabastria and A. tenera) have washed in distilled water. Female genitalia recently been reported from Iran of which were put in spot dish in a drop of glycerin the last two species were new records for for illustration. Iranian fauna (Khayrandish et al. 2012). The

Khayrandish et al. 19

Figure 1. Geographic map of the collected species of Ametastegia in the North of Iran. A. alabastria (), A. pallipes (▲), A. persica (), A. tenera ().

Figures 2–5. Sampling localities in North of Iran: 2. Eshmankomachal, Astaneyeh Ashrafiyeh, , 3. Ziaz, Rahimabad, , Gilan province, 4. , Rahimabad, Rudsar, Gilan province, 5. Qazychak, Rahimabad, Rudsar, Gilan province.

20 Study of the genus Ametastegia in Iran

Digital photos of whole specimens were Results taken with a JVC KYF-F75U camera In this survey, a total of 271 specimens (86 attached to a Leica Z6 APO zoom system females and 185 males) of the genus or alternatively with a Leica DFC450 C Ametastegia were collected from northern camera attached to a Leica M205 C Iran consisting of four species, of which stereomicroscope. Lighting was either one species is new to science (Table 1). from a cold light source attached to double light guides or from a reversed Genus Ametastegia Costa, 1882 LED ring light. The specimens were Ametastegia Costa, 1882: 198. Type species: illuminated indirectly by diffused light Ametastegia fulvipes Costa, 1882 reflected from the inner surface of a Aomodyctium Ashmead, 1898: 309. Type species: styrofoam cup or a styrofoam hemisphere Strongylogaster abnormis Provancher, 1885. set up around the specimen. Ovipositors Protemphytus Rohwer, 1909: 92. Type species: Emphytus coloradensis Weldon, 1907. and aedeagus valves were imaged with Ametastegia (Emphytina) Rohwer, 1911: 399-400. the KYF-F75U camera attached to an Type species: Emphytina pulchella Rohwer, Olympus BX51 compound microscope. 1911 Composite images with an extended Simplemphytus MacGillivray, 1914: 363. Type depth of field were created using the species: Simplemphytus pacificus MacGillivray, software CombineZ5, CombineZP or 1914 alternatively AutoMontage 5.01. Unitaxonus MacGillivray, 1921: 32. Type species: Unitaxonus repentinus MacGillivray, 1921 The specimens were identified mostly Ocla Malaise, 1957: 13. Type species: Ocla using the keys of Benson (1952), Smith albinigripes Malaise, 1957. (1979), Zhelochovtsev (1994) and Zombori (1982). The morphological terminology Key to the species of the genus used in this work follows Richards (1977). Ametastegia known from Iran Type material studied in this paper are 1- Forewing with cells 1R1 and IRs deposited in the following collections: separated by first free of radial sector veins TMUT = Tarbiat Modares University, (=Rs) (Fig. 6) (Subgenus Ametastegia)……..2 Faculty of Agriculture, Department of - Forewing with cells 1R1 and IRs fused, Entomology, Tehran, Iran; SDEI = first free of radial sector vein (=Rs) absent Senckenberg Deutsches Entomologisches (Fig. 7) (Subgenus Protemphytus) ………….3 Institut, Müncheberg, Germany; SBUK= 2- Abdomen entirely black...... A. glabrata Shahid Bahonar University, Faculty of - Abdomen yellow except the first black Agriculture, Department of Plant tergite (Figs. 12, 14)...... A. alabastria Protection, Kerman, Iran; IRIPP = Iranian Research Institute of Plant Protection, 3- Postspiracular sclerite white (Fig. 8), Tehran, Iran. lancet with 15 serrulae (Fig. 28) …………………………...... A. persica sp. n. The following taxonomic abbreviations - Postspiracular sclerite black (Fig. 9).….... 4 used in this study: 4- Hind legs and tegulae mostly white (Fig. POL: The distance between the inner 10); scutellum dull with dense surface margins of the lateral ocelli. striations between the punctures OOL: The distance from the lateral ocellus ……………………………………..A. pallipes to the inner margin of the compound eye. - Hind legs and tegulae entirely black (Fig. OCL: The distance from the lateral ocellus 11); scutellum usually more or less to the occipital carina or posterior margin punctate in the middle …………...A. tenera of the head.

Khayrandish et al. 21

Table 1. Updated list of the species of Ametastegia (Hym., Tenthredinidae) in Iran.

Scientific name Previous Collected New species Reference recorded species species Ametastegia alabastria * * Khayrandish et al. (2012); Ametastegia glabrata * Zirnegiebl (1956) Ametastegia pallipes * * Benson (1968); Lacourt (1999) Khayrandish et al. (2012); Ametastegia persica * * Current study Ametastegia tenera * * Khayrandish et al. (2012) Total 4 species 4 species 1 species

Ametastegia alabastria (Konow, 1898) 11.V.2011, 2♀, 2♂, 25.V.2011, 5♀, 3♂, 08.VI.2011, 1♂, 28.VI.2011, 1♀, 2♂, Taxonus alabastrius Konow, 1898: 238. 13.VII.2011, 11♂, 03.IIX.2011, 1♀, 3♂, leg.: Material examined: IRAN, Gilan M. Khayrandish. province: Qazvin- highway, Distribution: Armenia and Azerbaijan Stalkhjan, [36°56′30″N 49°32′54″E] 144 m, (Taeger and Blank 2011), Iran (Khayrandish 05.V.2011, 2♂, 10.V.2011, 1♀; Astaneyeh et al. 2012). Ashrafiyeh, Eshman Komachal, Larval host plant: Unknown. [37°22′06″N, 49°57′54″E] -1 m, 05.IV.2010, 4♂, 12.IV.2010, 1♀, 1♂, 19.IV.2010, 1♀, 2♂, Short description: Female. Length, 6.5–7.2 26.IV.2010, 1♀, 3♂, 10.V.2010, 1♀, 3♂, mm.; frontal crest, clypeus, labrum, 17.V.2010, 1♀, 24.V.2010, 3♀, 07.VI.2010, 2♀, mandibles, maxillae, labium, maxillary and 30.VIII.2010, 2♀, 06.IX.2010, 1♀, 13.IX.2010, labial palps yellow and other portion of head 4♀, 2♂, 20.IX.2010, 1♀, 1♂, 04.X.2010, 3♂, black; dorsal surface of thorax black except 11.X.2010, 1♀, 2♂, 18.X.2010, 5♀, 3♂, tegulae and lateroposterior of pronotum 25.X.2010, 3♀, 1♂, 01.XI.2010, 1♀, 1♂, yellow; cenchri dirty white; legs yellow, the 15.XI.2010, 1♀; Rudsar, Ziaz, [36°52′30″N end of hind tibiae a little dark and tarsi 50°13′24″E] 490 m, 12.IV.2010, 2♀, darker; upper part of pleura black and lower 26.IV.2010, 1, 10.V.2010, 1♂, 24.V.2010, 2♀, part yellow; sternum yellow, usually 31.V.2010, 2♀, 5♂, 07.VI.2010, 3♀, 12♂, mesosternum or sometimes section of it 14.VI.2010, 3♀, 6♂, 21.VI.2010, 1♂, black; abdomen yellow except the first basal 05.VII.2010, 1♂, 09.VIII.2010, 1♂, tergite black, sawsheath black (Figs. 12–13), 06.IX.2010, 2♂, 04.X.2010, 1♂; Rudsar, lancet with 16 serrulae (Figs. 16–17). Orkom, [36°45′42″N 50°18′12″E] 1235 m, Male. Length, 5.6–6.2 mm. Color similar to 12.IV.2010, 1♂, 26.IV.2010, 1♂, 17.V.2010, female except mesosternum yellow (Figs. 1♀, 2♂, 24.V.2010, 1♀, 07.VI.2010, 2♀, 14-15). Aedeagus valve as in Fig. 18. 14.VI.2010, 1♀, 1♂, 13.IX.2010, 1♂; Rudsar, Remarks. The specimens have been Qazychak, [36°45′54″N 50°19′36″E] 1803 m, collected from April to mid November. In 17.V.2010, 1♀, 31.V.2010, 1♂, 14.VI.2010, total, 149 specimens were collected at 1♂; Mazandaran province: Chamestan, altitudes from 0 to 700 m and others (12) Jourband, [36°26′18″N 52°07′12″E] 275 m, from higher altitudes. 158 specimens were 11.V.2011, 2♂, 25.V.2011, 6♂, 08.VI.2011, collected by Malaise traps, two specimens 1♂; Chamestan, Tangehvaz , [36°21′54″N rom Pteridium and the one from Rubus by 52°06′12″E] 687 m, 26.IV.2011, 3♀, 2♂, sweeping net.

22 Study of the genus Ametastegia in Iran

Figures 6–11. Ametastegia spp.: 6. Wing of subgenus Ametastegia; 7. Wing of subgenus Protemphytus; 8. Lateral view of thorax of Ametastegia persica sp. n.; 9. Lateral view of thorax of Ametastegia tenera; 10. Dorsal view of thorax of Ametastegia pallipes; 11. Dorsal view of thorax of Ametastegia tenera.

Khayrandish et al. 23

Figures 12–18. Ametastegia alabastria: 12. Female, dorsal view; 13. Female, lateral view; 14. Male, dorsal view; 15. Male, lateral view; 16–17. Ovipositor; 18. Aedeagus valve.

24 Study of the genus Ametastegia in Iran

Ametastegia pallipes (Spinola, 1808) Material examined: IRAN, Gilan province: Tenthredo pallipes Spinola, 1808: 19. Rudsar, Rahimabad, Ziaz, [36°52′30″N Tenthredo (Emphytus) grossulariae Klug, 1818: 50°13′24″E] 490 m, 07.VI.2010, 1♀; Rudsar, 283. Rahimabad, Orkom, [36°45′42″N Dolerus (Emphytus) leucopodus Lepeletier, 1823: 50°18′12″E] 1235 m, 10.V.2010, 1♀, 119. 17.V.2010, 1♀, 24.V.2010, 1♀, 07.VI.2010, 1♀; Dolerus leucopodus Serville, 1823: 56. Rudsar, Rahimabad, Qazychak, Tenthredo lapponica Zetterstedt, 1838: 350. [36°45′54″N 50°19′36″E] 1803 m, 07.VI.2010, Emphytus pallipes Provancher, 1878: 66–67 1♀, leg.: M. Khayrandish. Emphytus canadensis W.F. Kirby, 1882: 204. Emphytus pallidipes Dalla Torre, 1894: 119. Distribution: Widely distributed in the cavata MacGillivray, 1911: 305. European part of the West Palaearctic and Empria cetaria MacGillivray, 1921: 33–34. has reported from Canada and U.S.A in Emphytus hyacinthus MacGillivray, 1923: 16. Nearctic (Taeger and Blank 2011), Iran Emphytus hospitus MacGillivray, 1923: 15–16. (Benson 1968; Lacourt 1999; Khayrandish et Emphytus halesus MacGillivray, 1923: 13. al. 2012). Emphytus heroicus MacGillivray, 1923: 14–15. Larval host plants: Viola canina, V. odorata, Emphytus hiatus MacGillivray, 1923: 15. V. tricolor and Vicia (Taeger et al. 1998).

Figures 19–22. Ametastegia pallipes: 19. Female, dorsal view; 20. Female, lateral view; 21–22. Ovipositor.

Khayrandish et al. 25

Short description: Length 7.1–7.8 mm; 14.VI.2010; Gilan, Astaneyeh Ashrafiyeh, antenna and head black; apical part of Eshman Komachal, [37°22′06″N 49°57′54″E] clypeus sometimes brownish; labrum, –1 m, 6.IX.2010 1♂ (TMUT), 4.X.2010, 1♂ maxillary and labial palpi whitish; thorax (TMUT); Mazandaran, Nour, Chamestan, black; tegulae white; legs mostly white with Tangehvaz, [36°21′54″N 52°06′12″E] 687 m, base of each coxa black; one-fourth of hind 26.V.2011, 1♀ (SDEI), 28.VI.2011, 1♂ (SDEI), femur, apical part of hindtibia, and all tarsi 13.VII.2011, 3♂ (TMUT), 3.VIII.2011, 3♂ sometimes infuscated; abdomen black (Figs. (TMUT), 5.IX.2011, 1♂, 1♀ (TMUT); 19-20); clypeus approximately shallow, Mazandaran, Nour, Chamestan, Chmestan- circularly emarginated, malar space about Gaznasara road, [36°18′54″N, 52°07′48″E] twice diameter of front ocellus;; first free of 1291 m, 10.V.2011, 1♂ (TMUT), 9.VI.2011, 1♂ radial sector vein absent (Fig. 7); lancet with (SBUK), 1♀ (SDEI), 13.VII.2011, 1♂ (SBUK), 14 serrulae (Figs. 21–22). 1♂ (TMUT), 6.VIII.2011, 1♂ (SBUK), 1♂ Remarks: The specimens have been collected (SDEI); 16.VIII.2011, 1♂ (TMUT), Malaise in May and June by Malaise traps at various trap, leg., M. Khayrandish. altitudes (from 500–1800 m). Female. Length, 7.2–7.8 mm. (Figs. 23, 25, 27-28). Ametastegia persica Khayrandish, Talebi Colour. Head black, ventral surface of & Blank, sp. n. (Figs 23-30). apical antennomeres brown, labrum, Type material: HOLOTYPE, female, IRAN, maxillary and labial palpi and sometimes Gilan, Rudsar, Rahimabad, Orkom anterior edge of clypeus yellowish or dirty [36°45′42″N 50°18′12″E] 1235 m, 17.V.2010, yellow; medial part of mandible red and Malaise trap; leg., M. Khayrandish, shiny; thorax black, posterior margin of deposited in TMUT; Paratypes: Gilan, pronotum white, tegulae usually not Rudsar, Rahimabad, Orkom; 17.V.2010, 1♀ entirely black, antero-lateral area pale (SBUK), 1♂ (SDEI), 1♀ (TMUT), 31.V.2010, (sometimes completely black or one half of 2♀ (TMUT), 7.VI.2010, 1♂ (SBUK), 1♀ it white), post-spiracular sclerite white; (TMUT), 14.VI.2010, 1♂ (SBUK), 28.VI.2010, coxae black but in basement white, 2♂ (TMUT), 5.VII.2010, 1♂ (SBUK), 3♂ trochanter white, profemur white but two (TMUT), 12.VII.2010, 1♀ (TMUT), 7♂ third of inner surface dark brown (IRIPP), 26.VII.2010, 1♂ (TMUT), 9.VIII.2010, (sometimes only one third of inner surface 2♂ (SBUK), 1♂ (TMUT), 16.VIII.2010, 1♀ black), mesofemur like profemur but with a (SBUK), 1♂ (TMUT), 23.VIII.2010, 2♀, 2♂ pale brown border on outer (or without (SBUK), 2♂ (TMUT), 30.VIII.2010, 1♂ border), metafemur in one third of basal (TMUT), 6.IX.2010, 13.IX.2010; Gilan, white and the rest of it black (sometimes Rudsar, Rahimabad, Ziaz, [36°52′30″N half or two third of metafemur white and 50°13′24″E] 490 m, 17.V.2010, 1♀, 1♂ (SBUK), the rest black), protarsus and mesotarsus 1♀ (SDEI), 24.V.2010, 1♂ (SBUK), 1♂, 2♀ infuscated, metatarsus darker; wing (TMUT), 31.V.2010, 2♂ (SBUK), 3♂ 2♂ hyaline, veins brown, apex of costa near (TMUT), 7.VI.2010, 2♀, 4♂ (SBUK), 3♂ the stigma and stigma darker; abdomen (TMUT), 14.VI.2010, 4♂ (SBUK), 3♂ (TMUT), black, sawsheath and cerci black sometimes 21.VI.2010, 1♂ (TMUT), 28.VI.2010, 1♂ dark brown, saw almost yellow to pale (SBUK), 12.VII.2010, 3♂ (SBUK), 19.VII.2010, brown and shiny. 1♂ (SBUK), 26.VII.2010, 1♂ (SBUK), 1♂ Head. Distal edge of clypeus roundly (TMUT), 13.IX.2010, 1♂ (SBUK), 4♂ (TMUT); emarginate over complete width, Gilan, Rudsar, Rahimabad, Qazychak, emargination 0.19 times as deep as length of [36°45′54″N 50°19′36″E] 1803 m, 17.V.2010, clypeus, without tooth at center; malar

26 Study of the genus Ametastegia in Iran space ca 0.35 times as long as diameter of sclerite as well as different colors of legs. It front ocellus; lengths of flagellomeres about can also easily be separated from A. tenera 0.86: 0.61: 0.53: 0.3: 0.26: 0.25: 0.26 mm; by the white postspiracular sclerite and width of first and second flagellomeres 0.14 different colors of clypeus and pronotum. and 0.13 mm., respectively; camparison lengths (width) of flagellomeres about 1 Ametastegia tenera (Fallén, 1808) (0.16): 0.71 (0.15): 0.61: 0.34: 0.3: 0.29: 0.3; Tenthredo tenera Fallén, 1808: 109. third antennomere 1.4 × 4th and so longer Tenthredo (Emphytus) patellata Klug, 1818: 283– than three apical antennomeres together. 284. POL: OOL: OCL = 1: 1.65: 1.75. Dolerus (Emphytus) luctuosus Lepeletier, 1823: Thorax. Shiny and smooth except scutellum 119. punctate in the posterior half; coxae conical Dolerus (Emphytus) nigritus Lepeletier, 1823: and prominent, hind basitarsus subequal in 120. Dolerus nigritus Serville, 1823: 57. length to tarsomeres 2–5 combined, tibiae Dolerus luctuosus Serville, 1823: 57. with two apical spur that one of them a little Tenthredo trunculi Vallot, 1845: 51. bit longer than the other, longer foretibial Simplemphytus pacificus MacGillivray, 1914: 363– spur with a small tooth near the end, tarsal 364. claws bifid; forewing without first free Emphytina vanduzeei Rohwer, 1915: 205–206. sector of vein Rs (Fig. 7). Emphytus haustus MacGillivray, 1923: 14. Legs. Hind basitarsus subequal in length to Emphytus haliartus MacGillivray, 1923: 14. tarsomeres 2–5 combined. Empria columna MacGillivray, 1923: 54. Wing. Apex of costa near the stigma; Material examined: IRAN, Mazandaran Forewing without first free sector of vein province, Nour, Chamestan, Gaznasara, Rs. [36°16′54″N 52°10′54″E] 2035 m, 26.V.2011, 1♀, Abdomen. Shiny and smooth; cerci small Malaise trap, leg., M. Khayrandish. and one segmented; sawsheath shiny, Distribution: Widely distributed in the smooth and straight, rounded at apex; European part of the West Palaearctic, lancet with 15 serrulae (Figs. 27–28). eastwards to China and has reported from Male. Length, 5.8–7.2 mm. Color similar to Canada and U.S.A in Nearctic (Taeger and female except two third apical of hind Blank 2011), Iran (Khayrandish et al. 2012). basitarsus brown to dark (Figs. 24, 26). Larval host plants: Rumex and Cirsium are Structure similar to that of female but the main host plants for A. tenera but the malar space ca 0.3 times as long as front larvae burrow into the various plant stems ocellus and POL: OOL: OCL = 1: 1.6: 1.65; to pupate. Therefore some of host plants aedeagus valve as in Figs. 29–30. may be due to erroneous information Distribution: Iran (Gilan and Mazandaran (Taeger et al. 1998). provinces). Short description: Body length 6 mm; Larval host plant: Unknown entirely black (Figs. 31–32); apical of fore Etymology. The species name, persica, is femur and fore tibia whitish; wings derived from its geographic range of uniformly, lightly infuscated; clypeus distribution: Iran, known formerly as Persia. slightly deep, V-shaped emarginated, Diagnosis. This species is closely to malar space nearly twice diameter of front Ametastegia pallipes and A. carpini, but it can ocellus, first free of radial sector vein be easily distinguished from the both absent (Fig. 7); lancet with 14 serrulae (Figs. species by having the white postspiracular 33–34).

Khayrandish et al. 27

Figures 23–30. Ametastegia persica sp. n.: 23. Female, dorsal view; 24. Male, dorsal view; 25. Female, lateral view; 26. Male, lateral view; 27–28. Ovipositor; 29. Aedeagus valve (left); 30. Aedeagus valve (right).

28 Study of the genus Ametastegia in Iran

Figures 31–34. Ametastegia tenera: 31. Female, dorsal view; 32. Female, lateral view; 33–34. Ovipositor. 1998; Taeger and Blank 2011), England

Discussion (Benson 1952; Liston 1995; Taeger and Blank 2011), Russia (Zhelochovtsev 1994; Liston Taeger and Blank (2011) listed two species of 1995; Taeger and Blank 2011) and Turkey the genus Ametastegia occurring in Iran (A. (Benson, 1968; Çalmasur and Özbek 2004; glabrata and A. pallipes). Khayrandish et al. Taeger and Blank 2011) fauna with three (2012) reported three species of this genus (e. common species. A. alabastria has only been g. A. pallipes, A. tenera and A. alabastria). With reported from Armenia and Azerbaijan description a new species (A. persica sp. n.) in (Taeger and Blank 2011) but other species the present study, the number of recorded have been reported from many countries and species of Ametastegia in Iran have been regions (Taeger and Blank 2011). Within the increased to five. Collected species in this species of Ametastegia, A. alabastria with 161 study are common in some countries from specimens (58% of all Ametastegia collected West Palaearctic, for example two species specimens) is the most abundant species of similar to France, Germany, England, Russia this genus in northern Iran (Fig. 35) followed (Liston 1995; Taeger and Blank 2011), and by A. persica sp. n. (38%). Number of Turkey fauna (Benson 1968; Çalmasur and Ametastegia species in the West Palaearctic Özbek 2004; Taeger and Blank 2011), 1 increa se to eleven with description of A. species with Greece, Armenia and persica sp. n. Most probably, some other Azerbaijan (Taeger and Blank 2011). The species of Ametastegia may distributed in Iran present study revealed that Iran’s Ametastegia (similar to reported species from neighboring fauna has the most similarity with France countries or the Palaearcic region), which (Liston 1995; Lacourt 1999; Taeger and Blank needs further studies. 2011), Germany (Liston 1995; Taeger et al.

Khayrandish et al. 29

Figure 35. Comparison of abundance of Ametastegia species in the North of Iran.

Acknowledgments Museum (Natural History), Entomology, We cordially thank to three anonymous 22(4): 111–207. reviewers and the editor (Dr. Ebrahim CABI, 2007. Crop Protection Compendium Ebrahimi) for their valuable comments and (2007 edition), CAB International. suggestions on the earlier version of this Available from: http://www.cabi.org paper. We also thank Dr. Ahmad Nadimi, /compendia/cpc/index.htm (accessed Dr. Abbas Mohammadi, Mr. Vahid 10 December 2012). Khosravi, Safaverdi, Mr. Ghorbani, Mr. Çalmasur, Ö. and Özbek, H. 2004. A Contribution to the Knowledge of the Falakian, Mr. Hasanzadeh and Mr. Moadi Tenthredinidae (Hymenoptera: Symph- for their support during the field work. We yta) Fauna of Turkey Part II: Subfamilies are grateful to Dr. Andreas Taeger and Mr. Blennocampinae, Dolerinae, Nematinae Andrew Liston for helps and useful and Selandrinae. Turkish Journal of guidance. This research was supported by Zoology, 28: 55–71. the Department of Entomology of the Chevin, H. 1979. Les differents tenthre-des Tarbiat Modares University in Tehran, Iran recontrees sur coursons de vigne. Phytoma, and the Senckenberg Deutsches Entomolo- 305: 29–30. gisches Institut in Müncheberg, Germany. Costa, A. 1882. Rapporto preliminare e sommario sulle ricerche zoologiche fatte References in Sardegna durante la primav-era del 1882. Rendiconto dell' Accademia delle Ashmead, W.H. 1898. Classification of the Scienze Fisiche e Matematiche, 1. Ser. 21: horntails and sawflies, or the sub-order 189–201. Phytophaga. Canadian Entomologist, 30: Dalla Torre, K.W. (= Dalla Torre, C.G. de) 305–316. 1894. Catalogus Hymenopterorum Benson, R.B. 1952. Hymenoptera, hucusque descriptorum systematicus et Symphyta. Handbooks for the synonymicus. Vol. 1: Tenthredinidae identification of British , 6 (2b): 51– incl. Uroceridae (Phyllophaga and 137. Xylophaga). Sumptibus Guilelmi Benson, R.B. 1968. Hymenoptera from Engelmann, Lipsiae, 459 pp. Turkey, Symphyta. Bulletin of the British

30 Study of the genus Ametastegia in Iran

Fallén, C.F. 1808. Försok till uppställning Middle West. Bulletin of the Brooklyn och beskrifning å de i Sverige fundne Entomological Society, 18: 53–56. Arter af Insect-Slägtet Tenthredo L. MacGillivray, A.D. 1923. A Century of Kongliga Svenska Vetenskaps Academiens Tenthredinoidea. University of Illinois nya Handlingar, 29 (2): 98–124. Bulletin, 20 (50): 1–38. Khayrandish, M., Talebi, A.A., Blank, S.M. Malipatil, M.B., Naumann I.D. and and Fathipour, Y. 2012. Study of Williams D.G. 1995. First record of dock common sawflies of the genus Ametastegia glabrata (Fallén) in Ametastegia (Hymenoptera: Tenthred- Australia (Hymenoptera: Tenthredin- inidae) in north of Iran with two new idae. Australian Journal of Entomology, species records. Proceedings of the 20th 34: 95–96. Iranian plant protection congress, pp. Malaise, R. 1957. Some Neotropical and 161. Oriental Tenthredinoidea (Hym.). Kirby, W.F. 1882. List of Hymenoptera Entomologisk Tidskrift, 78(1): 6–22. with Descriptions and Figures of the Myers, N., Mittermeier R.A., Mittermeier Typical Specimens in the British CG, da Fonseca GAB, Kent J. 2000. Museum. 1. Tenthredinidae and Siricidae Biodiversity hotspot for conservation Vol. 1. By order of the Trustees, priorities. Nature, 403: 853–858. London, 450 pp. Naumann, I.D., Williams, M.A. and Klug, F. 1818. Die Blattwespen nach ihren Schmidt, S. 2002. Synopsis of the Gattungen und Arten zusammen- Tenthredinidae (Hymenoptera) in gestellt. Gesellschaft Naturfor-schender Australia, including two newly recorded, Freunde zu Berlin Magazin für die introduced sawfly species associated with Neuesten Entdeckungen in der Gesammten willlows (Salix spp.). Australian Journal of Naturkunde 8: 273–307. Entomology, 41: 1–6. Konow, F.W. 1898. Neue Tenthrediniden. Provancher, L. 1878. Les Insectes. Wiener Entomologische Zeitung, 17(7–8): Hyménoptères. 1. Fam. des Tenthredi- 228–238. nides Tenthredinidae. Le Nat-uraliste Lacourt, J. 1999. Répertoire des Tenthredin- Canadien, 10: 65–73. idae ouest-paléarctiques (Hymenoptera: Richards, O. W. 1977. Hymenoptera. Symphyta). Annales de la Société Introduction and key to families. entomologique de France, 3: 1–432. Handbooks for the Identification of British Lepeletier, A.L.M. (=Le Peletier de Saint- Insects, 6 (1): 1–38. Fargeau, A.) 1823. Monographia Rohwer, S.A. 1909. Notes on Tenthre- Tenthredinetarum synonymia extricata. dinoidea, with descriptions of new Apud Auctorem [etc.], Parisiis, 176 pp. species (Paper III). Canadian Entomologist, Liston, A. D. 1995. Compendium of 41: 88–92. European Sawflies. List of species, Rohwer, S.A. 1911. New sawflies in the modern nomenclature, distribution, food collections of the United States National plants, identification literature. Chalastos Museum. Proceedings of the United States Forestry, Gottfrieding, 190 pp. National Museum, 41: 377–411. MacGillivray, A.D. 1911. New species of Rohwer, S.A. 1915. Descriptions of new Empria. I. Eastern Species. Cannadian species of Hymenoptera. Proceedings of Entomologist, 43 (9): 305–311. the United States National Museum, 49: MacGillivray, A. D. 1914. New genera and 205–249. species of sawflies. Cannadian Entomologist, Serville, A. 1823. Hyménoptères. pp. 1–96, 46 (10): 363–367. In: Vieillot P., Desmarest A.G., De MacGillivray, A. D. 1921. New species of Blainiville P.C., Serville A. and Emphytinae and Selandriinae, Hymen- Lepelletier S.-F. (Eds.), Faune Française, optera. Psyche, 28(2): 31–35. ou Histoire Naturelle, Générale et MacGillivray, A.D. 1923. New species of Particuliére, des Animaux qui se Trouvent Tenthredinidae from the East and

Khayrandish et al. 31

en France, Livr. 7 and 8, Chez Rapet, from:http://www.sdei.de/ecatsym/in Paris. dex.html (accessed 3 January 2013). Smith, D.R. 1979. Nearctic sawflies. IV. Taeger, A., Blank, S.M. and Liston, A.D. Allantinae: Adults and larvae 2010. World Catalog of Symphyta (Hymenoptera: Tenthredinidae). United (Hymenoptera). Zootaxa, 2580: 1–1064. States Department of Agriculture, Technic- Vallot, J.N. 1845. Sur les tenthredes de la al Bulletin, 1595: 1–172. vigne. Mémoires de l'Académie des Spinola, M. 1808. Insectorum liguriae inscriptions et belles-lettres, 1843–1844: species novae aut rariores, quas in agro 50–52. Ligustico nuper detexit, descripsit, Wei, M. and Nie, H. 1998. Generic list of eticonibus illustravit Maximilianus Tenthredinoidea s. str. (Hymenoptera) Spinola, adjecto catalogo specierum in new systematic arrangement with auctoribus jam enumeratarum, quae in synonyms and distribution data. (In eadem regione passim occurrunt. Vol. English, abstract in Chinese). Journal of 2(1)(1807), 2(2–4)(1808), Symptibus Central South Forestry, University, Auctoris, Typis Yves Gravier, Genuae, Zhuzhou 18(3): 23-31. 262 pp. Zetterstedt, J.W. 1838. Ordo IV. Storozhenko, S.Y. and Kuznetsov, V.N. Hymenoptera. pp. 326–358, In: 1995. Pest insects of the Russian Far Zetterstedt J.W. (Ed.), Insecta Lapponica East. Vladivostok: Dalnauka, 276 pp. Descripta. Sectio Secunda, L. Voss, Taeger, A., Altenhofer, E., Blank, S.M., Lipsiae. Jansen, E., Kraus, M., Pschorn-Walcher, Zhelochovtsev, A.N. 1994. 27. Order H. and Ritzau, C. 1998. Kommentare zur Hymenoptera. Suborder Symphyta Biologie, Verbreitung und Gefährdung (Chalastogastra). pp. 1–387, In: Med- der Pflanzenwespen Deutschlands vedev, G.S. (Ed.), Keys to the Insects of the (Hymenoptera, Symphyta). pp. 49–135, European Part of the USSR. Oxonian Press In: Taeger, A. and Blank, S.M. (Eds.), Pvt. Ltd., New Delhi. Pflanzenwespen Deutschlands (Hymen- Zirngiebl, L. 1956. Blattwespen aus Iran. optera: Symphyta). Kommentierte Bestan- Mitteilungen der Münchner Entomol- dsaufnahme. Goecke & Evers, Keltern. ogischen Gesellschaft, 46: 322–326. Taeger, A. and Blank, S.M. 2011. Zombori, L. 1982. The European Genera of ECatSym—Electronic World Catalog of Blennocampinae (Hymenoptera: Sym- Symphyta (Insecta: Hymenoptera). phyta: Tenthredinidae). Acta Zoologica Program version 3.10, data version 38 Academiae Scientiarum Hungaricae, 28 (1- (07.12.2011). Digital Entomological 2): 183–192. Information, Müncheberg, Available

32 Study of the genus Ametastegia in Iran

ﻣﻄﺎﻟﻌﻪ ﺟﻨﺲ (Ametastegia (Hymenoptera: Tenthredinidae: Allantinae در ﺷﻤﺎل اﻳﺮان ﻫﻤﺮاه ﺑﺎ ﺗﻮﺻﻴﻒ ﻳﻚ ﮔﻮﻧﻪ ﺟﺪﻳﺪ

ﻣﺤﻤﺪ ﺧﻴﺮ اﻧﺪﻳﺶ1، ﻋﻠﻲ اﺻﻐﺮ ﻃﺎﻟﺒﻲ1، اﺳﺘﻔﺎن م. ﺑﻼﻧﻚ2، ﻳﻌﻘﻮب ﻓﺘﺤﻲ ﭘﻮر1

1 ﮔﺮوه ﺣﺸﺮه ﺷﻨﺎﺳﻲ، داﻧﺸﻜﺪه ﻛﺸﺎورزي، داﻧﺸﮕﺎه ﺗﺮﺑﻴﺖ ﻣﺪرس، ﺻﻨﺪوق ﭘﺴﺘﻲ 336-14115، ﺗﻬﺮان، اﻳﺮان 2 ﻣﺆﺳﺴﻪ ﺣﺸﺮه ﺷﻨﺎﺳﻲ زﻧﻜﻨﺒﺮگ آﻟﻤﺎن، ﺧﻴﺎﺑﺎن اﺑﺮﺳﻮاﻟﺪر 90، 15374، ﻣﻮﻧﺸﻨﺒﺮگ، آﻟﻤﺎن * ﭘﺴﺖ اﻟﻜﺘﺮوﻧﻴﻜﻲ ﻧﻮﻳﺴﻨﺪه ﻣﺴﺌﻮل ﻣﻜﺎﺗﺒﻪ: [email protected] ﺗﺎرﻳﺦ درﻳﺎﻓﺖ: 11 ﺷﻬﺮﻳﻮر 1394، ﺗﺎرﻳﺦ ﭘﺬﻳﺮش: 14 آﺑﺎن 1394، ﺗﺎرﻳﺦ اﻧﺘﺸﺎر: 24 آﺑﺎن 1394

ﭼﻜﻴــﺪه: زﻧﺒﻮرﻫــﺎي ﺟــﻨﺲ Hymenoptera: ) Ametastegia Costa 1882 Tenthredinidae: Allantinae) در ﺷﻤﺎل اﻳﺮان ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﻗﺮار ﮔﺮﻓﺖ. ﻧﻤﻮﻧﻪﻫﺎ ﺑـﺎ اﺳﺘﻔﺎده از ﺗﻮر ﺣﺸﺮهﮔﻴﺮي و ﺗﻠﻪ ﻣﺎﻟﻴﺰ در ﻃﻲ ﻣﺎهﻫﺎي اﺳﻔﻨﺪ 1388 ﺗـﺎ آﺑـﺎن 1389 و اﺳﻔﻨﺪ 1389 ﺗﺎ آﺑﺎن1390 ﺟﻤﻊ آوري ﺷﺪﻧﺪ. ﭼﻬﺎر ﮔﻮﻧـﻪ از اﻳـﻦ ﺟـﻨﺲ ﺟﻤـﻊ آوري و ﺷﻨﺎﺳـﺎﻳﻲ ﮔﺮدﻳـﺪ ﻛـﻪ از ﺑـﻴﻦ آن ﻫـﺎ ﮔﻮﻧـﻪ ,Ametastegia persica Khayrandish Talebi & Blank sp. n. ﺑﻪ ﻋﻨﻮان ﮔﻮﻧﻪ ﺟﺪﻳﺪ ﺑﺮاي دﻧﻴﺎي ﻋﻠـﻢ ﺗﻮﺻـﻴﻒ ﺷـﺪ. ﻛﻠﻴـﺪ ﻣﺼﻮر ﺑﺮاي ﺷﻨﺎﺳﺎﻳﻲ ﮔﻮﻧﻪﻫﺎي ﺟﻨﺲ Ametastegia در اﻳﺮان ﺗﻬﻴﻪ ﮔﺮدﻳﺪ.

واژﮔﺎن ﻛﻠﻴﺪي: Symphyta، ﮔﻮﻧﻪ ﺟﺪﻳﺪ، ﻛﻠﻴﺪ ﺷﻨﺎﺳﺎﻳﻲ، ردهﺑﻨﺪي