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Chapter 28 *Lecture Powepoint
Chapter 28 *Lecture PowePoint The Female Reproductive System *See separate FlexArt PowerPoint slides for all figures and tables preinserted into PowerPoint without notes. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Introduction • The female reproductive system is more complex than the male system because it serves more purposes – Produces and delivers gametes – Provides nutrition and safe harbor for fetal development – Gives birth – Nourishes infant • Female system is more cyclic, and the hormones are secreted in a more complex sequence than the relatively steady secretion in the male 28-2 Sexual Differentiation • The two sexes indistinguishable for first 8 to 10 weeks of development • Female reproductive tract develops from the paramesonephric ducts – Not because of the positive action of any hormone – Because of the absence of testosterone and müllerian-inhibiting factor (MIF) 28-3 Reproductive Anatomy • Expected Learning Outcomes – Describe the structure of the ovary – Trace the female reproductive tract and describe the gross anatomy and histology of each organ – Identify the ligaments that support the female reproductive organs – Describe the blood supply to the female reproductive tract – Identify the external genitalia of the female – Describe the structure of the nonlactating breast 28-4 Sexual Differentiation • Without testosterone: – Causes mesonephric ducts to degenerate – Genital tubercle becomes the glans clitoris – Urogenital folds become the labia minora – Labioscrotal folds -
Chapter 24 Primary Sex Organs = Gonads Produce Gametes Secrete Hormones That Control Reproduction Secondary Sex Organs = Accessory Structures
Anatomy Lecture Notes Chapter 24 primary sex organs = gonads produce gametes secrete hormones that control reproduction secondary sex organs = accessory structures Development and Differentiation A. gonads develop from mesoderm starting at week 5 gonadal ridges medial to kidneys germ cells migrate to gonadal ridges from yolk sac at week 7, if an XY embryo secretes SRY protein, the gonadal ridges begin developing into testes with seminiferous tubules the testes secrete androgens, which cause the mesonephric ducts to develop the testes secrete a hormone that causes the paramesonephric ducts to regress by week 8, in any fetus (XX or XY), if SRY protein has not been produced, the gondal ridges begin to develop into ovaries with ovarian follicles the lack of androgens causes the paramesonephric ducts to develop and the mesonephric ducts to regress B. accessory organs develop from embryonic duct systems mesonephric ducts / Wolffian ducts eventually become male accessory organs: epididymis, ductus deferens, ejaculatory duct paramesonephric ducts / Mullerian ducts eventually become female accessory organs: oviducts, uterus, superior vagina C. external genitalia are indeterminate until week 8 male female genital tubercle penis (glans, corpora cavernosa, clitoris (glans, corpora corpus spongiosum) cavernosa), vestibular bulb) urethral folds fuse to form penile urethra labia minora labioscrotal swellings fuse to form scrotum labia majora urogenital sinus urinary bladder, urethra, prostate, urinary bladder, urethra, seminal vesicles, bulbourethral inferior vagina, vestibular glands glands Strong/Fall 2008 Anatomy Lecture Notes Chapter 24 Male A. gonads = testes (singular = testis) located in scrotum 1. outer coverings a. tunica vaginalis =double layer of serous membrane that partially surrounds each testis; (figure 24.29) b. -
Oogenesis in Mammals
OOGENESIS IN MAMMALS In contrast to most other vertebrates , mammals do not replenish the stores of oocytes present in the ovary at birth. At birth the human ovaries contain about 1 million oocytes ( many of which are already degenerating) that have been arrested in the diplotene stage of the first meiotic division . These oocytes are already surrounded by a layer of follicular cells or granulosa cells , and the complex of ovum and its surrounding cellular investments is known as a follicle . Of all the germ cells present in the ovary ,only about 400 (one per menstrual cycle) will reach maturity and become ovulated. The remainder develop to varying degrees and then undergo atresia (degeneration). Oocytes first become associated with follicular cells in the late fetal period , when they are going through early prophase of the first meiotic division . The primary oocyte (so called because it is undergoing the first meiotic division ) plus its incomplete covering of flattened follicular cells is called a primordial follicle . According to Gougeon (1993) a follicle passes through three major phases on its way to ovulation. The first phase is characterized by a large pool of nongrowing follicles , approximately 500,000 per ovary at birth. In this pool are primordial follicles, which develop into primary follicles by surrounding themselves with a complete single layer of cuboidal follicular cells . By this time , the oocytes have entered the first period of meiotic arrest , the diplotene stage . In human , essentially all oocytes , unless Page 1 of 5 : SEM-2 (GEN ) , Unit#6 : OOGENESIS : Pritha Mondal they degenerate ,remain arrested in the diplotene stage until puberty ; some will not progress past the diplotene stage until the woman’s last reproductive cycle (age 45 to 55 years). -
GROSS and HISTOMORPHOLOGY of the OVARY of BLACK BENGAL GOAT (Capra Hircus)
VOLUME 7 NO. 1 JANUARY 2016 • pages 37-42 MALAYSIAN JOURNAL OF VETERINARY RESEARCH RE# MJVR – 0006-2015 GROSS AND HISTOMORPHOLOGY OF THE OVARY OF BLACK BENGAL GOAT (Capra hircus) HAQUE Z.1*, HAQUE A.2, PARVEZ M.N.H.3 AND QUASEM M.A.1 1 Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh 2 Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong 3 Department of Anatomy and Histology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Basherhat, Dinajpur * Corresponding author: [email protected] ABSTRACT. Ovary plays a vital 130.07 ± 12.53 µm and the oocyte diameter role in the reproductive biology and was 109.8 ± 5.75 µm. These results will be biotechnology of female animals. In this helpful to manipulate ovarian functions in study, both the right and left ovaries of small ruminants. the Black Bengal goat were collected from Keywords: Morphometry, ovarian the slaughter houses of different Thanas follicles, cortex, medulla, oocyte. in the Mymensingh district. For each of the specimens, gross parameters such as INTRODUCTION weight, length and width were recorded. Then they were processed and stained with Black Bengal goat is the national pride of H&E for histomorphometry. This study Bangladesh. The most promising prospect revealed that the right ovary (0.53 ± 0.02 of Black Bengal goat in Bangladesh is g) was heavier than the left (0.52 ± 0.02 g). that this dwarf breed is a prolific breed, The length of the right ovary (1.26 ± 0.04 requiring only a small area to breed and cm) was lower than the left (1.28 ± 0.02 with the advantage of their selective cm) but the width of the right (0.94 ± 0.02 feeding habit with a broader feed range. -
The Discovery of Different Types of Cervical Mucus and the Billings Ovulation Method
The Discovery of Different Types of Cervical Mucus and the Billings Ovulation Method Erik Odeblad Emeritus Professor, Dept. of Medical Biophysics, University of Umeå, Sweden Published with permission from the Bulletin of the Ovulation Method Research and Reference Centre of Australia, 27 Alexandra Parade, North Fitzroy, Victoria 3068, Australia, Volume 21, Number 3, pages 3-35, September 1994. Copyright © Ovulation Method Research and Reference Centre of Australia 1. Abstract 2. Introduction 3. Anatomy and Physiology 4. What is Mucus? 5. The Commencement of my Research 6. The Existence of Different Types of Crypts and of Mucus 7. Identification and Description of G, L, and S Mucus 8. G- and G+ Mucus 9. Age, Pregnancy, the Pill and Microsurgery 10. P Mucus 11. F Mucus 12. The Role of the Vagina 13. The Different Types of Secretions and the Billings Ovulation Method 14. Early Infertile Days 15. The Days of Possible Fertility 16. Late Infertile Days 17. Anovulatory Cycles 18. Lactation 19. Diseases and the Billings Ovulation Method 20. The Future 21. Acknowledgements 22. Author's Note 23. References 24. Appendix Abstract An introduction to and some new anatomical and physiological aspects of the cervix and vagina are presented and also an explanation of the biosynthesis and molecular structure of mucus. The history of my discoveries of the different types of cervical mucus is given. In considering my microbiological investigations I suspected the existence of different types of crypts and cervical mucus and in 1959 1 proved the existence of these different types. The method of examining viscosity by nuclear magnetic resonance was applied to microsamples of mucus extracted 1 outside of several crypts. -
Regulation and Roles of the Hyaluronan System in Mammalian Reproduction
REPRODUCTIONREVIEW Regulation and roles of the hyaluronan system in mammalian reproduction Ali A Fouladi-Nashta1, Kabir A Raheem1,2, Waleed F Marei1,3, Fataneh Ghafari1 and Geraldine M Hartshorne4 1Royal Veterinary College, Reproduction Research Group, Hawkshead Campus, Hatfield, UK, 2Department of Veterinary Surgery and Theriogenology, Michael Okpara University of Agriculture, Umudike, Nigeria, 3Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt and 4Warwick Medical School, University of Warwick, Coventry, UK and Centre for Reproductive Medicine, University Hospitals Coventry and Warwickshire NHS Trust, Coventry, UK Correspondence should be addressed to A A Fouladi-Nashta; Email: [email protected] Abstract Hyaluronan (HA) is a non-sulphated glycosaminoglycan polymer naturally occurring in many tissues and fluids of mammals, including the reproductive system. Its biosynthesis by HA synthase (HAS1–3) and catabolism by hyaluronidases (HYALs) are affected by ovarian steroid hormones. Depending upon its molecular size, HA functions both as a structural component of tissues in the form of high-molecular-weight HA or as a signalling molecule in the form of small HA molecules or HA fragments with effects mediated through interaction with its specific cell-membrane receptors. HA is produced by oocytes and embryos and in various segments of the reproductive system. This review provides information about the expression and function of members of the HA system, including HAS, HYALs and HA receptors. We examine their role in various processes from folliculogenesis through oocyte maturation, fertilisation and early embryo development, to pregnancy and cervical dilation, as well as its application in assisted reproduction technologies. Particular emphasis has been placed upon the role of the HA system in pre-implantation embryo development and embryo implantation, for which we propose a hypothetical sequential model. -
Ans 214 SI Multiple Choice Set 4 Weeks 10/14 - 10/23
AnS 214 SI Multiple Choice Set 4 Weeks 10/14 - 10/23 The following multiple choice questions pertain to material covered in the last two weeks' lecture sets. Answering the following questions will aid your exam preparation. These questions are meant as a gauge of your content knowledge - use them to pinpoint areas where you need more preparation. 1. A heifer begins ovarian activity at A. 6-8 months B. 8-10 months C.10-12 months D. 12-14 months E. 24 months 2. The gestation length of a cow is A. 82 days C. 166 days D. 283 days E. 311 days 3. All of the following produce hormones vital to ovarian cyclicity EXCEPT A. hypothalamus B. ovary C. posterior pituitary D. uterus E. all of the above are correct 4. Which of the following structures is INCORRECTLY matched to the hormones it produces? A. uterus: PGF2a B. ovary: testosterone, activin, estrogen, oxytocin C. placenta: progesterone, testosterone, estrogen D. anterior pituitary: ACTH, FSH, LH E. hypothalamus: GnRH, CRH 5. In the female reproductive system of all species A. the ovaries are supported by the mesometrium B. urine can only exit via the urethra via the suburethral diverticulum C. the uterus produces progesterone D. the oviduct is supported by the mesosalpinx E. the ovary is directly connected to the oviduct 6. Which of the following is FALSE about the mare? A. Ovulates from the medulla because of an inverted ovarian structure B. Ovulates a 2n oocyte C. Does not have a suburethral diverticulum D. Ovulates at only one site on the ovary, called the ovulation fossa E. -
And Theca Interna Cells from Developing Preovulatory Follicles of Pigs B
Differential production of steroids by dispersed granulosa and theca interna cells from developing preovulatory follicles of pigs B. K. Tsang, L. Ainsworth, B. R. Downey and G. J. Marcus * Reproductive Biology Unit, Department of Obstetrics and Gynecology and Department of Physiology, University of Ottawa, Ottawa Civic Hospital, Ottawa, Ontario, Canada Kl Y 4E9 ; tAnimal Research Centre, Agriculture Canada, Ottawa, Ontario, Canada K1A 0C6; and \Department of Animal Science, Macdonald College of McGill University, Ste Anne de Bellevue, Quebec, Canada H9X ICO Summary. Dispersed granulosa and theca interna cells were recovered from follicles of prepubertal gilts at 36, 72 and 108 h after treatment with 750 i.u. PMSG, followed 72 h later with 500 i.u. hCG to stimulate follicular growth and ovulation. In the absence of aromatizable substrate, theca interna cells produced substantially more oestrogen than did granulosa cells. Oestrogen production was increased markedly in the presence of androstenedione and testosterone in granulosa cells but only to a limited extent in theca interna cells. The ability of both cellular compartments to produce oestrogen increased up to 72 h with androstenedione being the preferred substrate. Oestrogen production by the two cell types incubated together was greater than the sum produced when incubated alone. Theca interna cells were the principal source of androgen, predominantly androstenedione. Thecal androgen production increased with follicular development and was enhanced by addition of pregnenolone or by LH 36 and 72 h after PMSG treatment. The ability of granulosa and thecal cells to produce progesterone increased with follicular development and addition of pregnenolone. After exposure of developing follicles to hCG in vivo, both cell types lost their ability to produce oestrogen. -
Spermatozoa After Sperm Residence in the Female Reproductive Tract E
Detection of altered acrosomal physiology of cryopreserved human spermatozoa after sperm residence in the female reproductive tract E. Z. Drobnis, P. R. Clisham, C. K. Brazil L. W. Wisner, C. Q. Zhong and J. W. Overstreet ^Division of Reproductive Biology and Medicine, Department of Obstetrics and Gynecology, School of Medicine, and department of Reproduction, School of Veterinary Medicine, University of California, Davis, CA 95616-8659, USA At least some of the spermatozoa that remain motile following cryopreservation have sustained sublethal damage that reduces their functional capacity in vivo. Although it is believed that acrosomal damage is partly responsible for impaired sperm function in vivo, direct evidence for this hypothesis is lacking because spermatozoa have not been collected from the female reproductive tract for evaluation. In the study reported here, cervical mucus was collected from women 24 h after artificial insemination by cervical cup. For both cryopreserved and nonfrozen inseminates, spermatozoa within the cervical mucus and spermatozoa that migrated out of mucus into culture medium (t = 1 h) were viable and had intact acrosomes. However, although nonfrozen spermatozoa did not initially respond to induction of the acrosome reaction with follicular fluid, a significant proportion of cryopre- served spermatozoa did respond. These results demonstrate that cryopreservation increases the acrosomal lability of spermatozoa residing in the female reproductive tract. An in vitro test was developed to detect this form of cryodamage. Sperm-free mucus was collected before insemination and spermatozoa from the inseminate were allowed to swim into this column of mucus in vitro. Spermatozoa recovered from this mucus sample were compared with spermatozoa from the paired sample collected from the cervix 24 h later. -
Interactions Between Oocyte and Surrounding Cumulus Cells Influence the Results of Assisted Reproduction Fritzsche H, Michelmann HW, Siebzehnrübl E Schmedemann RKA J
Journal für Reproduktionsmedizin und Endokrinologie – Journal of Reproductive Medicine and Endocrinology – Andrologie • Embryologie & Biologie • Endokrinologie • Ethik & Recht • Genetik Gynäkologie • Kontrazeption • Psychosomatik • Reproduktionsmedizin • Urologie Interactions between Oocyte and Surrounding Cumulus Cells Influence the Results of Assisted Reproduction Fritzsche H, Michelmann HW, Siebzehnrübl E Schmedemann RKA J. Reproduktionsmed. Endokrinol 2006; 3 (6), 373-378 www.kup.at/repromedizin Online-Datenbank mit Autoren- und Stichwortsuche Offizielles Organ: AGRBM, BRZ, DVR, DGA, DGGEF, DGRM, D·I·R, EFA, OEGRM, SRBM/DGE Indexed in EMBASE/Excerpta Medica/Scopus Krause & Pachernegg GmbH, Verlag für Medizin und Wirtschaft, A-3003 Gablitz Interactions between Oocyte and Surrounding Cumulus Cells Influence the Results of Assisted Reproduction H. Fritzsche1, H. W. Michelmann2, E. Siebzehnrübl3, R. K. A. Schmedemann4 The interactions between oocyte and surrounding cumulus cells, as well as between cumulus oophorus and theca cells, were investigated in IVF/ ICSI cycles. Gap junctions connect cumulus cells with the oocyte, thereby enabling a bi-directional exchange of products essential for optimal oocyte development. GnRH, FSH, LH and E2 play a major role during oocyte maturation. In general, FSH and LH are prerequisites for folliculogenesis, as well as oogenesis, but it is the quantitative threshold value of both that seems to determine oocyte quality and pregnancy rate. It remains to be determined how apoptosis and the anti-Muellerian hormone (AMH) can be used as predictive factors regarding the success of ART. In a retrospec- tive sub-analysis of comparative stimulation regimens, using either LH + FSH (hMG-HP) or FSH (rFSH) alone in GnRH-antagonist down-regulated cycles, it was possible to demonstrate that stimulation with LH and FSH results in a significantly higher pregnancy rate in IVF-patients compared to a stimulation with only FSH. -
Extracellular Vesicles
Human Reproduction Update, Vol.22, No.2 pp. 182–193, 2016 Advanced Access publication on December 9, 2015 doi:10.1093/humupd/dmv055 Extracellular vesicles: roles in gamete maturation, fertilization and embryo Downloaded from https://academic.oup.com/humupd/article-abstract/22/2/182/2457903 by University of California, San Diego user on 17 April 2019 implantation Ronit Machtinger1,*, Louise C. Laurent2, and Andrea A. Baccarelli3 1Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Sheba Medical Center and Tel-Aviv University, Tel Hashomer 52561, Israel 2Department of Reproductive Medicine, Division of Maternal Fetal Medicine, University of California, San Diego, CA, USA 3Departments of Environmental Health and Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA *Correspondence address. Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Sheba Medical Center and Tel-Aviv University, Tel Hashomer 52561, Israel. E-mail: [email protected] Submitted on May 13, 2015; resubmitted on November 3, 2015; accepted on November 9, 2015 table of contents ........................................................................................................................... † Introduction † Methods † Extracellular vesicles † Extracellular vesicles and sperm maturation † Extracellular vesicles, communication in the ovarian follicle and oocyte maturation † Extracellular vesicles in fertilization † Extracellular vesicles -
Most Fertilizing Mouse Spermatozoa Begin Their Acrosome Reaction Before Contact with the Zona Pellucida During in Vitro Fertilization
Most fertilizing mouse spermatozoa begin their acrosome reaction before contact with the zona pellucida during in vitro fertilization Mayuko Jina, Eiji Fujiwarab, Yasutaka Kakiuchia,c, Masaru Okabed, Yuhkoh Satouhd,e, Shoji A. Babaa, Kazuyoshi Chibaa,f, and Noritaka Hirohashia,c,f,1 aDepartment of Biological Sciences, cDepartment of Genetic Counseling, and fGlycoscience Institute, Ochanomizu University, Tokyo 112-8610, Japan; bDocumentary Channel Co., Kawaguchi, Saitama 333-0844, Japan; and dResearch Institute for Microbial Disease and eWorld Premier International Research Center (WPI) Immunology Frontier Research Center, Osaka University, Osaka 565-0871, Japan Edited* by Ryuzo Yanagimachi, Institute for Biogenesis Research, University of Hawaii, Honolulu, HI, and approved February 16, 2011 (received for review December 5, 2010) To fuse with oocytes, spermatozoa of eutherian mammals must spermatozoa within the cumulus at various stages of the AR in pass through extracellular coats, the cumulus cell layer, and the various species has also been noted by several investigators (11– zona pellucida (ZP). It is generally believed that the acrosome 14). This prompted us to reexamine the site of the AR in those reaction (AR) of spermatozoa, essential for zona penetration and mouse spermatozoa that actually participate in fertilization. We fusion with oocytes, is triggered by sperm contact with the zona used a video microscopic in vitro fertilization (VMIVF) system, pellucida. Therefore, in most previous studies of sperm–oocyte which allowed us to determine the site of the AR in fertilizing interactions in the mouse, the cumulus has been removed before mouse spermatozoa, retrospectively (Fig. 1). We used double- insemination to facilitate the examination of sperm–zona interac- transgenic male mice whose spermatozoa express enhanced green tions.