DOCSLIB.ORG

Structural Analysis of Arabinoxylans Isolated from Ball-Milled Switchgrass Biomass

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Structural Analysis of Arabinoxylans Isolated from Ball-Milled Switchgrass Biomass Carbohydrate Research 345 (2010) 2183–2193 Contents lists available at ScienceDirect Carbohydrate Research journal homepage: www.elsevier.com/locate/carres Structural analysis of arabinoxylans isolated from ball-milled switchgrass biomass Koushik Mazumder, William S. York * Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA article info abstract Article history: Ball-milled alcohol-insoluble residue (AIR) was prepared from switchgrass (Panicum virgatum var Alamo) Received 16 June 2010 and sequentially extracted with 50 mM ammonium oxalate buffer, 50 mM sodium carbonate, 1 M KOH Received in revised form 20 July 2010 containing 1% NaBH4, and 4 M KOH containing 1% NaBH4. Arabinoxylan was the most abundant Accepted 22 July 2010 component of the 1 M KOH-extracted fraction, which was treated with endoxylanase to generate Available online 30 July 2010 oligosaccharides. Gel-permeation chromatography of these oligosaccharides produced three size- homogeneous oligosaccharide fractions with molecular weights of 678, 810, and 1074 Da, corresponding Keywords: to 5, 6, and 8 pentose units, respectively. Detailed structural analysis of these oligosaccharides was per- Switchgrass formed using methylation analysis, multiple-step mass spectrometry (ESIMSn), and 1D and 2D NMR spec- Enzymatic digestion Arabinoxylan oligosaccharides troscopy. The preferred gas-phase fragmentation pathways were identified for these oligosaccharides, Per-O-methylation providing extensive sequence information that was completely consistent with structures determined n Multiple-step mass spectrometry by ab initio NMR analysis. These results demonstrate the high information content of ESIMS analysis Structural analysis when applied to cell-wall-derived oligosaccharides and provide standard data that will facilitate the anal- ysis of cell-wall polysaccharide fragments with a sensitivity that is sufficient for the analysis of samples obtained from dissected tissues as well as other small samples. Ó 2010 Published by Elsevier Ltd. 1. Introduction along the xylan backbone) have been correlated to altered cell wall properties.3 This paper describes development and application of The bioconversion of lignocellulosic biomass to liquid fuels is a analytical methods for the detailed structural analysis of arabin- key emerging technology for addressing the need for environmen- oxylans from grasses, specifically the arabinoxylan of switchgrass, tally friendly and sustainable energy sources. Lignocellulosic bio- which has enormous potential as a biofuel crop. mass is a complex composite of many different polysaccharides, Structural characterization of polysaccharides such as arabinoxy- proteins, and phenolic polymers derived primarily from the cell lan involves identification of their constituent monosaccharide walls of grasses and woody plants. Although cellulose, pectin, and units, the monosaccharide sequence, the linkage position for each hemicellulose are well established as the three main classes of poly- glycosidic bond and the presence and location of each branch point. saccharides in the cell wall, the exact composition of the cell wall Owing to this inherent complexity, carbohydrate characterization varies considerably both within and between plant species. Consid- has required the use of diverse analytical methods, among which erable attention is currently being focused on switchgrass (Panicum NMR spectroscopy has played a major role. Recently, highly sensi- virgatum) as a potential source of lignocellulosic biomass in amounts tive, high-throughput methods for the analysis of glycan structure sufficient to support industrial-scale production of biofuels. have been developed using combinations of mass spectrometry, Arabinoxylans, which are major components of the cell walls of HPLC, and digestion with specific exo- and endo-glycosidases.4,5 P. virgatum and other grasses, have a backbone consisting of We now report the characterization of oligosaccharide fragments (1?4)-linked b-D-Xylp residues, some of which bear various side generated by the enzymatic digestion of aribinoxylans solubilized 1,2 chains (including a-L-Araf, a-L-Araf-(1?2)-a-L-Araf, and b-D- by alkali extraction of cell walls prepared from switchgrass biomass. Xylp-(1?2)-a-L-Araf) at O-2 and/or O-3. Arabinoxylans play a The composition and molecular masses of the purified oligosaccha- key role in maintaining the structural integrity of the cell walls rides were determined by GC–MS and MALDI-TOFMS, and detailed of these species. Understanding the detailed structure of arabin- structural information was obtained by glycosyl composition analy- oxylans in grasses is important in that differences in the molecular sis, glycosyl linkage analysis, multiple-stage electrospray-ionization features of these hemicellulosic polysaccharides (e.g., degree of mass spectrometry (ESIMSn) , and NMR spectroscopy. branching and spatial arrangement of arabinosyl substituents * Corresponding author. Tel.: +1 706 542 4401; fax: +1 706 542 4412. ESIMSn = multiple-stage electrospray-ionization mass spectrometry, where E-mail address: [email protected] (W.S. York). n = the number of times the isolation–fragmentation cycle has been carried out. 0008-6215/$ - see front matter Ó 2010 Published by Elsevier Ltd. doi:10.1016/j.carres.2010.07.034 2184 K. Mazumder, W. S. York / Carbohydrate Research 345 (2010) 2183–2193 2. Results and discussion Table 1 Methylation analysis data (molar ratios) of fractions 2, 3 and 4 2.1. Isolation of the arabinoxylan oligosaccharides Derivative Structurea Fraction 4 Fraction 3 Fraction 2 2,3,5-Me3 Ara T-Ara 0.8 0.8 1.2 Cell walls were prepared as an alcohol-insoluble residue (AIR) ob- 2,3,4-Me3 Xyl T-Xyl 0.8 1.2 1.0 tained by organic-solvent extraction of ball-milled switchgrass bio- 3,5-Me2 Ara 2-Ara - 0.9 - mass. AIR was extracted with 1 M KOH to solubilize arabinoxylan, 2,3-Me2 Xyl 4-Xyl 1.8 2.2 5.4 2-Me Xyl 3,4-Xyl 1.0 1.0 1.1 which was treated with endoxylanase to generate a mixture of xylo-oligosaccharides.6,7 The oligosaccharides were fractionated by a Residue structure and substituent sites within the oligosaccharide. (T indicates size-exclusion chromatography on Bio-Gel P-2 (fine) to yield a void terminal residue.) fraction and four partially included fractions (Fig. 1). are diagnostic for fragmentation events.17 Each free hydroxyl 2.2. Sugar composition analysis of the arabinoxylan group that is generated during gas-phase fragmentation of the oligosacccharides methylated oligosaccharide can be identified by its characteristic 14-Da mass difference relative to a methylated site. Such unme- The monosaccharide compositions of the switchgrass oligosac- thylated ‘scars’ facilitate the interpretation of the resulting MSn charide fractions were analyzed by high-performance anion-ex- data, providing key information required for the identification change chromatography8 (HPAEC) and gas chromatography–mass and location of branch points in the glycosyl sequence. The most spectrometry9,10 (GC–MS) of monosaccharides or monosaccharide abundant fragment ions in the ESIMSn spectra of the purified, derivatives, respectively, released by acid-catalyzed depolymeriza- per-O-methylated oligosaccharides were identified as Y and B frag- tion. The resulting arabinose/xylose ratios were 6.7, 2.1, and 3.9 for ment ions.17 Due to their high abundance, Y ions were most often fractions 2, 3, and 4, respectively. Fraction 5 contained only xylose. selected for secondary fragmentation in order to maximize ion counts for higher order MSn. 2.3. Methylation analysis of arabinoxylan oligosaccharides The ESIMS2 spectrum of per-O-methylated fraction 4 (Fig. 2A), recorded upon fragmentation of the quasimolecular ion at m/z Glycosidic linkage sites for each residue in the oligosaccharides 869, includes an abundant Y ion (m/z 695) generated by loss of a were determined by GC–MS analysis of partially methylated alditol single pentosyl residue from the precursor ion. Methylation analy- acetate (PMAA) derivatives obtained by per-O-methylation, hydro- sis (see above) indicated the presence of a single branched residue, lysis, reduction, and acetylation of each fraction.10–12 The results a nonreducing terminal xylosyl residue and a nonreducing termi- for fractions 2 and 4 (Table 1) are consistent with a backbone con- nal arabinosyl residue (see above). Therefore, two isomeric m/z sisting of (1?4)-linked xylopyranosyl residues and a single arabi- 695 ions can exist, one formed by loss of a terminal xylosyl residue nofuranosyl side chain linked to O-3 of an internal xylosyl residue. and the other formed by loss of a terminal arabinosyl residue. The For fraction 3 (Table 1), the results are also consistent with a ESIMS3 spectrum recorded upon fragmentation of the m/z 695 ion (1?4)-linked xylopyranosyl backbone, but with an Araf-(1?2)- is shown in Figure 2B. In this figure, each scar generated by the ini- Araf side chain. tial fragmentation giving rise to the precursor ion is indicated by an unrooted arrow, and the scar generated by subsequent frag- 2.4. Mass spectrometric analysis of the per-O-methylated mentation of the precursor ion is indicated by an arrow crossed oligosaccharides by a mass-labeled, dashed line. Fragmentation of the two isomeric precursor ion structures (m/z 695) generates two isomeric Y ions MALDI-TOF spectra of fractions 2, 3, and 4 (Supplementary (with three pentosyl residues with two scars) at m/z 521 and two Figs. S3, S2 and S1) included highly abundant quasi-molecular ions
Load more

Recommended publications
  • Isolation and Chemical Modification of Arabinoxylan and Galactoglucomannan
    THESIS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY Isolation and chemical modification of arabinoxylan and galactoglucomannan LINDA HÄRDELIN Department of Chemistry and Chemical Engineering CHALMERS UNIVERSITY OF TECHNOLOGY Gothenburg, Sweden 2018 Isolation and chemical modification of arabinoxylan and galactoglucomannan LINDA HÄRDELIN ISBN 978-91-7597-803-1 © LINDA HÄRDELIN, 2018 Doktorsavhandling vid Chalmers tekniska högskola Ny serie nr: 4484 ISSN 0346-718X Department of Chemistry and Chemical Engineering Chalmers University of Technology SE-412 96 Gothenburg Sweden Telephone + 46 (0)31-772 1000 Cover: SEM micrograph of arabinoxylan foam, taken together with A. Mårtensson Printed by Chalmers Reproservice Gothenburg, Sweden 2018 Isolation and chemical modification of arabinoxylan and galactoglucomannan LINDA HÄRDELIN Department of Chemistry and Chemical Engineering Chalmers University of Technology ABSTRACT Today’s society is based on the use of fossil fuels, where oil is used as transportation fuel and as raw material in the petrochemical industry. Efforts are made to increase the use of bio-based materials, since oil is a non- renewable source and a greenhouse gas contributor. The raw materials used in this thesis are agricultural and forestry by- products. Alkaline extraction was used to isolate the hemicellulose arabinoxylan (AX) from barley husk, while the hemicellulose galactoglucomannan (GGM) was obtained through concentration of pulp process water. Hemicelluloses can be used to produce materials. One way to increase the processability of the hemicellulose is to perform chemical modifications on it. By doing so, the properties of hemicellulose can be altered, such as decreased glass transition temperature (Tg), which will change the ability to process the hemicellulose into material.
    [Show full text]
  • Arabinoxylan-Oligosaccharides Act As Damage Associated Molecular
    ORIGINAL RESEARCH published: 07 August 2020 doi: 10.3389/fpls.2020.01210 Arabinoxylan-Oligosaccharides Act Edited by: Martin Heil, as Damage Associated Molecular Instituto Polite´ cnico Nacional de Me´ xico (CINVESTAV), Mexico Patterns in Plants Regulating Reviewed by: Johannes Werner Stratmann, Disease Resistance University of South Carolina, United States † † Hugo Me´lida 1, Laura Bacete 1,2 , Colin Ruprecht 3 ,DiegoRebaque1,2,4,IrenedelHierro1,2, Maxime Versluys, ´ 1 ´ ´ 4 3† 1,2* KU Leuven, Belgium Gemma Lopez ,Frederic Brunner , Fabian Pfrengle and Antonio Molina *Correspondence: 1 Centro de Biotecnolog´ıa y Geno´ mica de Plantas, Universidad Polite´ cnica de Madrid (UPM)—Instituto Nacional de Antonio Molina Investigacio´ n y Tecnolog´ıa Agraria y Alimentaria (INIA), Pozuelo de Alarco´ n (Madrid), Spain, 2 Departamento de [email protected] Biotecnolog´ıa-Biolog´ıa Vegetal, Escuela Te´ cnica Superior de Ingenier´ıa Agrono´ mica, Alimentar´ıa y de Biosistemas, UPM, †Present address: Madrid, Spain, 3 Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany, 4 ´ Laura Bacete, PlantResponse Biotech S.L., Campus de Montegancedo UPM, Pozuelo de Alarcon (Madrid), Spain Faculty of Natural Sciences, Institute for Biology, Norwegian University of Science and Technology, Immune responses in plants can be triggered by damage/microbe-associated molecular Trondheim, Norway patterns (DAMPs/MAMPs) upon recognition by plant pattern recognition receptors Colin Ruprecht, (PRRs). DAMPs are signaling molecules synthesized by plants or released from host Department of Chemistry, University of Natural Resources and Life Sciences, cellular structures (e.g., plant cell walls) upon pathogen infection or wounding. Despite the Vienna, Austria hypothesized important role of plant cell wall-derived DAMPs in plant-pathogen Fabian Pfrengle, Department of Chemistry, University of interactions, a very limited number of these DAMPs are well characterized.
    [Show full text]
  • Molecular Characterization of Arabinoxylans from Hull-Less Barley Milling Fractions
    Molecules 2011, 16, 2743-2753; doi:10.3390/molecules16042743 OPEN ACCESS molecules ISSN 1420-3049 www.mdpi.com/journal/molecules Article Molecular Characterization of Arabinoxylans from Hull-Less Barley Milling Fractions Xueling Zheng *, Limin Li and Xiaoxi Wang Grain College, Henan University of Technology, Zhengzhou 450052, Henan Province, China; E-Mails: [email protected] (L.L.); [email protected] (X.W.) * Author to whom correspondence should be addressed; E-mail: [email protected] or [email protected]; Tel.: +86-371-67789766; +86-317-67789306. Received: 27 December 2010; in revised form: 11 March 2011 / Accepted: 17 March 2011 / Published: 24 March 2011 Abstract: Arabinoxylans were prepared from different hull-less barley milling fractions (bran, shorts and flour). The yields of hull-less bran arabinoxylan (HBB-AX), shorts arabinoxylan (HBS-AX) and flour arabinoxylan (HBF-AX) were 8.42%, 4.08% and 2.13% respectively. Sugar composition analysis showed that arabinose and xylose were the main sugars. HBF-AX had the highest Ara/Xyl ratio, followed by HBS-AX and HBB-AX. Size exclusion chromatography analysis (HPSEC) showed that HBF-AX had the highest molecular weight, followed by HBS-AX and HBB-AX, which had the lowest molecular weight. Aqueous solutions of HBB-AX, HBS-AX and HBF-AX had higher molecular weight(Mw) than in 0.5 mol/L NaOH and 1.0 mol/L NaOH. Dynamic light scattering analysis (DLS) showed that HBB-AX, HBS-AX and HBF-AX had existed in two states in distilled water, 0.5 mol/LNaOH, 1.0 mol/L NaOH, and DMSO/H2O (90/10), an unaggregated state and an aggregated state, with the latter predominating.
    [Show full text]
  • Dietary Fibre from Whole Grains and Their Benefits on Metabolic Health
    nutrients Review Dietary Fibre from Whole Grains and Their Benefits on Metabolic Health Nirmala Prasadi V. P. * and Iris J. Joye Department of Food Science, University of Guelph, Guelph, ON N1G 2W1, Canada; [email protected] * Correspondence: [email protected] Received: 31 August 2020; Accepted: 30 September 2020; Published: 5 October 2020 Abstract: The consumption of whole grain products is often related to beneficial effects on consumer health. Dietary fibre is an important component present in whole grains and is believed to be (at least partially) responsible for these health benefits. The dietary fibre composition of whole grains is very distinct over different grains. Whole grains of cereals and pseudo-cereals are rich in both soluble and insoluble functional dietary fibre that can be largely classified as e.g., cellulose, arabinoxylan, β-glucan, xyloglucan and fructan. However, even though the health benefits associated with the consumption of dietary fibre are well known to scientists, producers and consumers, the consumption of dietary fibre and whole grains around the world is substantially lower than the recommended levels. This review will discuss the types of dietary fibre commonly found in cereals and pseudo-cereals, their nutritional significance and health benefits observed in animal and human studies. Keywords: dietary fibre; cereals; pseudo-cereals; chronic diseases 1. Introduction Consumers worldwide are interested in a healthy diet. Whole grain products, encompassing both cereals and pseudo-cereals, should constitute an important part of this healthy diet. The consumption of whole grain products is considered to have a beneficial effect on risk reduction of non-communicable diseases (NCD), including cardiovascular diseases, cancers, gastrointestinal disorders and type 2 diabetes [1–3].
    [Show full text]
  • Characterisation of Dietary Fibre in Cereal Grains and Products
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Epsilon Open Archive Characterisation of Dietary Fibre in Cereal Grains and Products Emphasis on Triticale and Rye Allah Rakha Faculty of Natural Resources and Agricultural Sciences Department of Food Science Uppsala Doctoral Thesis Swedish University of Agricultural Sciences Uppsala 2011 Acta Universitatis agriculturae Sueciae 2011:82 Cover: Triticale and rye grains (photo: Roger Andersson) ISSN 1652-6880 ISBN 978-91-576-7626-9 © 2011 Allah Rakha, Uppsala Print: SLU Service/Repro, Uppsala 2011 Characterisation of Dietary Fibre in Cereal Grains and Products – Emphasis on Triticale and Rye Abstract Utility of cereals is mainly defined by their composition. High content of extractable dietary fibre (DF) with retained molecular features may be desired for human consumption to derive certain benefits associated with DF. In contrast, low amount of extractable DF with degraded molecules will give higher feed value to cereals intended for animal feed. This thesis investigated the composition of DF in cereals, particularly triticale and rye products. Processing effects on extractable DF components, e.g. arabinoxylan (AX), β-glucan and fructan, were also examined. The structure of AX and β-glucan in triticale, barley and tritordium and the rheology of triticale extracts as influenced by content and extractability, molecular size and structure of AX were analysed. DF in triticale spanned a relatively narrow range (13-16%), with significant cultivar and location effects. Unfavourable growing conditions resulted in significantly lower molecular weights of AX, β-glucan and fructan in triticale. On the whole, triticale DF profile was more similar to wheat than rye.
    [Show full text]
  • Absence of Branches from Xylan in Arabidopsis Gux Mutants Reveals Potential for Simplification of Lignocellulosic Biomass
    Absence of branches from xylan in Arabidopsis gux mutants reveals potential for simplification of lignocellulosic biomass Jennifer C. Mortimera, Godfrey P. Milesa, David M. Brownb,1, Zhinong Zhanga, Marcelo P. Seguraa, Thilo Weimara, Xiaolan Yua, Keith A. Seffenc, Elaine Stephensd,2, Simon R. Turnerb, and Paul Dupreea,3 aDepartment of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom; bFaculty of Life Science, University of Manchester, Manchester M13 9PT, United Kingdom, cDepartment of Engineering, University of Cambridge, Cambridge CB2 1PZ, United Kingdom; and dDepartment of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom Edited by Chris R. Somerville, University of California, Berkeley, CA, and approved August 17, 2010 (received for review April 23, 2010) As one of the most abundant polysaccharides on Earth, xylan will Xylan plays a key role in human and animal nutrition and in provide more than a third of the sugars for lignocellulosic biofuel industrial uses of plant biomass. It is largely indigestible by humans production when using grass or hardwood feedstocks. Xylan is and can improve passage of material through the gut, thereby characterized by a linear β(1,4)-linked backbone of xylosyl residues helping to reduce the incidence of diseases such as colorectal substituted by glucuronic acid, 4-O-methylglucuronic acid or arab- cancer and type II diabetes (5). Conversely, highly substituted, in- inose, depending on plant species and cell types. The biological digestible xylan in animal feed leads to a loss of nutrition. During role of these decorations is unclear, but they have a major influ- paper and pulp manufacture, xylan MeGlcA residues are converted ence on the properties of the polysaccharide.
    [Show full text]
  • Feruloylation and Structure of Arabinoxylan in Wheat Endosperm Cell Walls from Rnai Lines with Suppression of Genes Responsible for Backbone Synthesis and Decoration
    CORE Metadata, citation and similar papers at core.ac.uk Provided by Rothamsted Repository Plant Biotechnology Journal (2017) 15, pp. 1429–1438 doi: 10.1111/pbi.12727 Feruloylation and structure of arabinoxylan in wheat endosperm cell walls from RNAi lines with suppression of genes responsible for backbone synthesis and decoration Jackie Freeman, Jane L. Ward, Ondrej Kosik, Alison Lovegrove, Mark D. Wilkinson, Peter R. Shewry and Rowan A.C. Mitchell* Plant Biology and Crop Science, Rothamsted Research, Harpenden, Hertfordshire, UK Received 2 February 2017; Summary revised 10 March 2017; Arabinoxylan (AX) is the major component of the cell walls of wheat grain (70% in starchy accepted 13 March 2017. endosperm), is an important determinant of end-use qualities affecting food processing, use for *Correspondence (Tel +44 1582 763133; animal feed and distilling and is a major source of dietary fibre in the human diet. AX is a fax +44 1582 763010; email heterogeneous polysaccharide composed of fractions which can be sequentially extracted by [email protected]) water (WE-AX), then xylanase action (XE-AX) leaving an unextractable (XU-AX) fraction. We determined arabinosylation and feruloylation of AX in these fractions in both wild-type wheat and RNAi lines with decreased AX content (TaGT43_2 RNAi, TaGT47_2 RNAi) or decreased arabinose 3-linked to mono-substituted xylose (TaXAT1 RNAi). We show that these fractions are characterized by the degree of feruloylation of AX, <5, 5–7 and 13–19 mg bound ferulate (gÀ1 AX), and their content of diferulates (diFA), <0.3, 1–1.7 and 4–5mg(gÀ1 AX), for the WE, XE and XU fractions, respectively, in all RNAi lines and their control lines.
    [Show full text]
  • In Vitro Colon Fermentation of Soluble Arabinoxylan Is Modified Through
    ORIGINAL RESEARCH published: 25 August 2021 doi: 10.3389/fnut.2021.707763 In vitro Colon Fermentation of Soluble Arabinoxylan Is Modified Through Milling and Extrusion Teresa Demuth 1, Veronica Edwards 1,2, Lea Bircher 2, Christophe Lacroix 2, Laura Nyström 1*† and Annelies Geirnaert 2† 1 Laboratory of Food Biochemistry, Institute of Food, Nutrition and Health, ETH Zurich, Zurich, Switzerland, 2 Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zurich, Zurich, Switzerland Dietary fibers such as arabinoxylan (AX) are promising food constituents to prevent particular diet-related chronic diseases because of their prebiotic properties. Arabinoxylan fermentation by the gut microbiota depends on the structural architecture of AX, which can be modified during food processing and consequently affect its prebiotic potential, but it is little investigated. Therefore, the aim of this study was to evaluate the effects of naturally occurring and processing-induced structural alterations of the in vitro Edited by: soluble AX of wheat bran and rye flour on the human colon fermentation. It Alfonso Benítez-Páez, was found that fermentation behavior is strongly linked to the AX fine structure and Principe Felipe Research Center their processing-induced modifications. The short-chain fatty acid (SCFA) metabolism, (CIPF), Spain acidification kinetics, bacterial growth, and bacterial composition revealed that wheat Reviewed by: Carlos Gómez-Gallego, bran AX (WBAX) was fermented faster than rye flour AX. Increased levels of bound University of Eastern Finland, Finland phenolic acids resulting from processing were identified as the inhibiting factor for AX Luc Saulnier, fermentation kinetics. Bacterial genera promoted by AX varied between AX source Institut National de la Recherche Agronomique (INRA), France and processing type, but also between microbiota.
    [Show full text]
  • Bio-Based Films from Wheat Bran Feruloylated Arabinoxylan: Effect of Extraction Technique, Acetylation and Feruloylation
    Carbohydrate Polymers 250 (2020) 116916 Contents lists available at ScienceDirect Carbohydrate Polymers journal homepage: www.elsevier.com/locate/carbpol Bio-based films from wheat bran feruloylated arabinoxylan: Effect of extraction technique, acetylation and feruloylation Secil Yilmaz-Turan a, Amparo Jim´enez-Quero a, Carolin Menzel a, Danila Morais de Carvalho b,c, Mikael E. Lindstrom¨ b,e, Olena Sevastyanova b,e, Rosana Moriana b,d,f, Francisco Vilaplana a,e,* a Division of Glycoscience, Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm, Sweden b Department of Fibre and Polymer Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm, Sweden c Department of Food and Nutrition, Faculty of Agriculture and Forestry, University of Helsinki, Helsinki, Finland d RISE Bioeconomy, Research Institutes of Sweden, Stockholm, Sweden e Wallenberg Wood Science Center, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm, Sweden f Department of Molecular Sciences, SLU Swedish University of Agricultural Sciences, Uppsala, Sweden ARTICLE INFO ABSTRACT Keywords: This study demonstrates the potential of feruloylated arabinoxylan (AX) from wheat bran for the preparation of Arabinoxylan bioactive barrier films with antioxidant properties. We have comprehensively evaluated the influence of the Wheat bran structural features and chemical acetylation of feruloylated AX extracted by subcritical water on their film Subcritical water extraction properties, in comparison with alkaline extracted AX and a reference wheat endosperm AX. The degree of Ferulic acid substitution (DS) of AX had a large influenceon filmformation, higher DS yielded better thermal and mechanical Acetylation Barrier properties properties.
    [Show full text]
  • Degradation Products of Complex Arabinoxylans by Bacteroides Intestinalis Enhance the Host Immune Response
    microorganisms Article Degradation Products of Complex Arabinoxylans by Bacteroides intestinalis Enhance the Host Immune Response Taro Yasuma 1,†, Masaaki Toda 1,†, Ahmed M. Abdel-Hamid 2,3,†, Corina D’Alessandro-Gabazza 1,2, Tetsu Kobayashi 4, Kota Nishihama 1, Valeria Fridman D’Alessandro 1 , Gabriel V. Pereira 2,5,6 , Roderick I. Mackie 2,5,6, Esteban C. Gabazza 1,2,* and Isaac Cann 2,5,6,7,8 1 Department of Immunology, Mie University, Tsu City 514, Mie, Japan; [email protected] (T.Y.); [email protected] (M.T.); [email protected] (C.D.-G.); [email protected] (K.N.); [email protected] (V.F.D.) 2 Carl R. Woese Institute for Genomic Biology (Microbiome Metabolic Engineering Theme), University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; [email protected] (A.M.A.-H.); [email protected] (G.V.P.); [email protected] (R.I.M.); [email protected] (I.C.) 3 Department of Botany and Microbiology, Faculty of Science, Minia University, El-Minia 61519, Egypt 4 Department of Pulmonary and Critical Care Medicine, Mie University, Tsu City 514, Mie, Japan; [email protected] 5 Department of Animal Science, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA 6 Energy Biosciences Institute, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA 7 School of Molecular and Cellular Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61874, USA Citation: Yasuma, T.; Toda, M.; 8 Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA Abdel-Hamid, A.M.; * Correspondence: [email protected] D’Alessandro-Gabazza, C.; † These authors contributed equally to this work.
    [Show full text]
  • Non-Starch Polysaccharides in Wheat Beers and Barley Malt Beers: a Comparative Study
    foods Article Non-Starch Polysaccharides in Wheat Beers and Barley Malt beers: A Comparative Study Miaomiao Li, Jinhua Du * and Yaxin Zheng College of Food Science and Engineering, Shandong Agricultural University, Tai’an, Shandong 271018, China; [email protected] (M.L.); [email protected] (Y.Z.) * Correspondence: [email protected]; Tel.: +86-538-8249157 Received: 30 December 2019; Accepted: 24 January 2020; Published: 27 January 2020 Abstract: Non-starch polysaccharides (NSPs) in beers attract extensive attention due to their health benefits. The aim of this work was to investigate and compare NSPs including arabinoxylan, arabinogalactan, β–glucans, and mannose polymers in wheat and barley malt beers as well as the influence on its quality. NSPs in wheat beers (1953–2923 mg/L) were higher than that in barley malt beers (1442–1756 mg/L). Arabinoxylan was the most abundant followed by arabinogalactan. In contrast to barley malt beers, wheat beers contained more mannose polymers (130–182 mg/L) than β-glucan (26–99 mg/L), indicating that more arabinoxylan, arabinogalactan, and mannose polymers came from wheat malt. The substitution degree of arabinoxylan in wheat beers (0.57–0.66) was lower than that in barley malt beers (0.68–0.72), while the degree of polymerization (38–83) was higher (p < 0.05) than that in barley malt beers (38–48), indicating different structures of arabinoxylan derived from barley malt and wheat malt. NSPs, especially arabinoxylan content, positively correlated (p < 0.01) with real extract and viscosity of beers. Furthermore, wheat and barley malt beers were well separated in groups by principal component analysis.
    [Show full text]
  • Molecular Features of Wheat Endosperm Arabinoxylan Inclusion in Functional Bread
    Foods 2013, 2, 225-237; doi:10.3390/foods2020225 OPEN ACCESS foods ISSN 2304-8158 www.mdpi.com/journal/foods Article Molecular Features of Wheat Endosperm Arabinoxylan Inclusion in Functional Bread Weili Li 1, Hui Hu 1, Qi Wang 2 and Charles S. Brennan 3,* 1 Department of Food and Tourism Management, Manchester Metropolitan University, Manchester, M14 6HR, UK; E-Mails: [email protected] (W.L.); [email protected] (H.H.) 2 Agriculture and Agri-Food Canada, Guelph Food Research Centre, Guelph N1G 5C9, Canada; E-Mail: [email protected] 3 Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln 7647, New Zealand * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +64-3-321-8236. Received: 26 March 2013; in revised form: 24 April 2013 / Accepted: 30 May 2013 / Published: 17 June 2013 Abstract: Arabinoxylan (AX) is a major dietary fibre component found in a variety of cereals. Numerous health benefits of arabinoxylans have been reported to be associated with their solubility and molecular features. The current study reports the development of a functional bread using a combination of AX-enriched material (AEM) and optimal commercial endoxylanase. The total AX content of bread was increased to 8.2 g per 100 g available carbohydrates. The extractability of AX in breads with and without endoxylanase was determined. The results demonstrate that water-extractable AX (WE-AX) increased progressively through the bread making process. The application of endoxylanase also increased WE-AX content. The presence of 360 ppm of endoxylanase had positive effects on the bread characteristics in terms of bread volume and firmness by converting the water unextractable (WU)-AX to WE-AX.
    [Show full text]