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Biomedicine : Biomedicine (general)

Kinter, Michael, Kinter, Caroline S. Application of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative A Replacement for Western Blot Analysis

Explains both the theoretical and experimental details of the selected reaction monitoring experiment as it is applied to ? Describes a new type of experiment called selected Springer reaction monitoring 2013, XIII, 65 p. 14 illus., Reviews the experiment result of a robust and powerful method to measure 1st 11 illus. in color. the abundance of a in complex samples edition A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions. The method that is most widely used is the Western blot analysis. While Western Printed book blot is a workhorse in laboratories studying protein expression and has several advantages, it Softcover also has a number of significant limitations. In particular, the method is semi-quantitative with Printed book limited dynamic range. Western blot focuses on a single protein per sample with only a small Softcover number of representative samples analyzed in an experiment. New quantitative tools have ISBN 978-1-4614-8665-7 been needed for some time to at least supplement, & possibly replace, the Western blot. Mass £ 54,99 | CHF 77,50 | 65,41 € | spectrometric methods have begun to compete with Western blot for routine quantitative 71,95 € (A) | 69,99 € (D) analyses of proteins. One of these methods is based on the tandem technique of selected reaction monitoring (SRM), which is also called multiple reaction Available monitoring (MRM). Discount group Science (SC) Order online at springer.com/booksellers Product category Springer Nature Customer Service Center GmbH Brief Customer Service Series Tiergartenstrasse 15-17 SpringerBriefs in Systems Biology 69121 Heidelberg Germany Other renditions T: +49 (0)6221 345-4301 Softcover [email protected] ISBN 978-1-4614-8667-1

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ISBN 978-1-4614-8665-7 / BIC: MBGR / SPRINGER NATURE: SCB0000X Part of