Carole Di Poi Broussard, Ph.D.

Current position: Postdoctoral Researcher in Marine Ecotoxicology & Ecophysiology Université de Caen Basse-Normandie CERMN UPRES EA 4258 - FR CNRS 3038 INC3M Phone: +33-629-668-274 UMR BOREA; MNHN, UPMC, UCBN, CNRS-7208, IRD-207 Email: [email protected] Esplanade de la Paix CS14032, F-14032 Caen, Cedex 5

RESEARCH FIELDS Invertebrate and Fish Ecology • Animal Behavior • Physiology and Neurobiology of Stress Aquatic Ecotoxicology • Physiology/Biochemistry of Detoxification and Oxidative Stress Regulation

MODEL ORGANISMS  Invertebrates: freshwater crustacean (e.g., daphnia), marine molluscs (e.g., bivalves, cephalopods)  Vertebrates: fishes (e.g., sea bass, threespine stickleback), marine mammals (e.g., sea lion, harbor seal)

TECHNICAL SKILLS  Husbandry: rearing of marine/freshwater fishes and invertebrates, algae cultures.  Behavioral analysis: field- and laboratory based studies (social and fitness-related behaviors, antipredator and predator behaviors).  Organismal bioassays: embryotoxicity and metamorphosis bioassays, immobilization and reproduction bioassays, physiological responses (e.g., in ovo ventilatory rate in cuttlefish embryos).  In vivo toxicity bioassays: biomarkers of cell oxidative stress and detoxification (catalase, GST), peroxidation of membranes (TBARS), neurotransmitter (monoamines, metabolites and receptors) and stress hormone (cortisol, corticosterone) quantification.  In vitro toxicity bioassays: cell viability (MTT test), stability of cell membranes (Neutral Red Retention assay), immunotoxicity (phagocytic activity, ROS production).  Technical skills: enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), High Performance Liquid Chromatography (HPLC), flow cytometry, RNA extraction and PCR, qRT-PCR.  Image analysis: GIMP and ImageJ softwares, Jwatcher (ethograms).  Bio-: Statview, Statistica, StatXact, soft

OTHER RELEVANT SKILLS  Management of research project, grant and funding proposal writing (in French and English)  Teaching and mentorship  Languages: English (fluent), French (native language) EDUCATION 2005-2008 Ph.D. in Neuro-Ethology of Fish ENES UMR 8195 Université Jean Monnet, Saint-Etienne (France) Funding: French Ministry of Higher Education and Research Supervisors: Pr. N. Mathevon and Dr. J. Attia Grade: Honors with oral congratulations

2004-2005 Master’s degree (2nd yr) in Physiology in Extreme Environments Université Claude Bernard, Lyon 1 (France)

2005 Training course in Cetology École Pratique des Hautes Études (EPHE), Montpellier (France)

2003-2004 Master’s degree (1st yr) in Marine Biology and Ecology Université de la Méditerranée, Marseilles (France)

2002-2003 License’s degree (3rd yr) in Marine Biology and Ecology Université de la Méditerranée, Marseilles (France)

2000-2002 License’s degree (2 yrs) in Life Sciences Université Joseph Fourier, Grenoble (France)

RESEARCH EXPERIENCE

January 2015-present Research fields: Marine Ecotoxicology, Ecophysiology CERMN UPRES EA 4258 - FR CNRS 3038 INC3M UMR BOREA; MNHN, UPMC, UCBN, CNRS-7208, IRD-207 Université de Caen Basse-Normandie, Caen (France)

April 2012-March 2014 Research fields: Marine Ecotoxicology, Behavioral Biology, Ecophysiology GMPc EA4259 UMR BOREA; MNHN, UPMC, UCBN, CNRS-7208, IRD-207 Université de Caen Basse-Normandie, Caen (France)

Dec. 2010-Dec. 2011 Research field: Behavioral Ecotoxicology, Ecophysiology GMPc EA4259 Université de Caen Basse-Normandie, Caen (France)

Oct. 2009-Nov. 2010 Research field: Genomics of Behavior Nadia Aubin-Horth’s Lab Institute of Integrative and Systems Biology Laval University, Quebec City (Canada)

Nov. 2008-Sept. 2009 Research fields: Behavioral Ecology, Endocrinology Shannon Atkinson’s Lab School of Fisheries and Ocean Sciences University of Alaska Fairbanks, Juneau (USA) FACULTY APPOINTMENTS

Sept. 2005-Sept. 2008 Sessional lecturer (242 hrs of teaching in Biology) Université Jean Monnet, Saint-Etienne (France)

Sept. 2011-June 2012 Sessional lecturer (30 hrs of teaching in Psychophysiology) Université de Caen Basse-Normandie, Caen (France)

RESEARCH FUNDING, GRANTS & AWARDS

2015-2016 DARM project (Pharmaceuticals, Personal Care Products and ecotoxicology in marine bivalves) funded by the Région Basse- Normandie (principal investigator: Dr. M.-P. Halm UCBN). (Post-doc salary for 15 months)

2011-2015 Pharm@Ecotox project (Pharmaceutical residues and marine ecotoxicology) funded by the Agence Nationale de la Recherche ANR (principal investigator: Dr. M.-P. Halm UCBN). (Post-doc salary for 24 months)

2010-2011 Postdoctoral Research Fellowship (Antidepressant residues and marine ecotoxicology in cephalopods) funded by the Université de Caen Basse- Normandie - 32.000 euros (principal investigator)

2010 Travel grant of the Canadian Society of Zoology - US$500

2009-2010 Government of Canada Postdoctoral Research Fellowship (PDRF) (Behavioral and molecular divergence in threespine stickleback) funded by the Government of Canada - CAD$32.000 (principal investigator)

2008-2009 Postdoctoral Fellowship (Maternal buffering of stress response in pinniped pups) funded by the Fyssen Foundation - 25.000 euros (principal investigator)

2008 Travel grant of the French Society for the Study of Animal Behavior (SFECA) - 250 euros

2007 Award for Ph.D. Excellence, Women in Sciences Program from L’Oréal France-UNESCO-French Academy of Science - 10.000 euros

OTHER RESEARCH ACTIVITIES

2013: Participation to the COST FA1301 “A network for improvement of cephalopod welfare and husbandry in research, aquaculture and fisheries (CephsInAction)”.

2011-2013: Student and Postdoc representative at the GMPc lab council (Université de Caen Basse-Normandie, Caen, France)

2010: Organization of the 5th Annual Meeting of the Canadian Society for Ecology and Evolution (Quebec City, Canada)

2005-2008: Participation to the COST 867 WELLFISH “Welfare of Fish in European Aquaculture”

Scientific Journal Reviewer: Biology Letters, Behavioural Brain Research, Aquatic Toxicology, etc.

Member to scientific societies: French Society for the Study of Animal Behavior (SFECA), Association for the Research in Toxicology (ARET), Society for Experimental Biology (SEB).

LIST OF PUBLICATIONS

 production: 1.6 articles/year

Forthcoming articles

5. Bidel F., Di Poi C. et al. Changes in cuttlefish brain following early postembryonic exposure to Venlafaxine (antidepressant): Neurodevelopmental and behavioral outcomes. In preparation. 4. Di Poi C., Halm-Lemeille M-.P., Deft P., Budzinski H., Darmaillacq A.-S., Bellanger C. Acute toxicity of the antidepressant fluoxetine in cuttlefish embryos. In preparation. 3. Di Poi C., Bélanger D., Amyot M., Rogers S., Aubin-Horth N. Evolution of four regulatory molecular networks associated with behaviour divergence in sticklebacks. Submitted (Current Biology). 2. Bidel F., Le Pabic C., Imarazene B., Koueta N., Di Poi C. et al. Biochemical and predatory behavior changes in juvenile cuttlefish (Sepia officinalis) following exposure to environmental concentrations of Venlafaxine (antidepressant). Submitted (Aquatic Toxicology; special issue: Behavioural Aquatic Toxicology). 1. Di Poi C., Lacasse J., Rogers S., Aubin-Horth N. Habitat-associated evolution of stress reactivity in sticklebacks. Submitted (Biology Letters).

Published articles

16. Bidel F., Di Poi C., Imarazene B., Koueta N., Budzinski H., Van Delft P., Bellanger B., Jozet-Alves C. In press. Pre-hatching fluoxetine-induced neurochemical, neurodevelopmental, and immunological changes in newly hatched cuttlefish. Environmental Science and Pollution Research. 15. Di Poi C., Hoover-Miller A., Blundell G., Atkinson S. 2015. Maternal buffering of stress response in free- ranging Pacific harbor seal pups in Alaska. Marine Mammal Science 31(3):1098-1117 (IF: 2.128). 14. Di Poi C., Lacasse J., Rogers S., Aubin-Horth N. 2014. Extensive behavioural divergence following colonisation of the freshwater environment in threespine sticklebacks. PLOS One 9(6):e98980 DOI: 10.1371/journal.pone.0098980 (IF: 4.09). 13. Di Poi C., Evariste L., Séguin A., Mottier A., Pedelucq J., Lebel J.-M., Serpentini A., Budzinski H., Costil K. 2014. Sub-chronic exposure to fluoxetine in juvenile oysters (Crassostrea gigas): uptake and biological effects. Environmental Science and Pollution Research DOI 10.1007/s11356-014-3702-1 (IF: 2.618). 12. Minguez L., Di Poi C., Farcy E., Ballandonne C., Benchouala A., Bojic C., Cossu-Leguille C., Costil K., Serpentini A., Lebel JM., Halm-Lemeille M.-P. 2014. Comparison of the sensitivity of seven marine and freshwater bioassays as regards antidepressant toxicity assessment. Ecotoxicology 23(9):1744-1754 (IF: 2.773). 11. Di Poi C., Bidel F., Dickel L., Bellanger C. 2014. Cryptic and biochemical responses in young cuttlefish Sepia officinalis exposed to environmentally relevant concentrations of fluoxetine. Aquatic Toxicology 151:36-45 (IF: 3.761). 10. Di Poi C., Bellanger C. 2014. Response to commentary on “Are some invertebrates exquisitely sensitive to the human pharmaceutical fluoxetine?”. Aquatic Toxicology 146:261-263 (IF: 3.730). 9. Di Poi C., Evariste L., Serpentini A., Halm-Lemeille M.-P., Lebel J.-M., Costil K. 2014. Toxicity of five antidepressant drugs on the embryo-larval development and metamorphosis in the Pacific oyster, Crassostrea gigas. Environmental Science and Pollution Research 21(23):13302-13314 (IF: 2.618). 8. Di Poi C., Darmaillacq A.-S., Dickel L., Boulouard M., Bellanger C. 2013. Effects of perinatal exposure to waterborne fluoxetine on memory processing in the cuttlefish Sepia officinalis. Aquatic Toxicology 132- 133:84-91 (IF: 3.761). 7. Di Poi C. 2012. The lament of Alaskan harbor seal pups: a story about maternal support. Annales de la Fondation Fyssen 27:62-77. 6. Attia J., Millot S., Di Poi C., et al. 2012. Demand feeding and welfare in farmed fish. Fish Physiology and Biochemistry 38:107-118 (IF: 1.607). 5. Di Poi C., et al. 2011. Biodiversity matters in a changing world. Biology Letters 7:4-6 (IF: 3.521). 4. Das K., Siebert U., Gillet A., Dupont A., Di Poi C., et al. 2008. Mercury immune toxicity in harbour seals: links to in vitro toxicity. Environmental Health 7:52 (IF: 2.480). 3. Millot S., Bégout M.-L., Person J., Breuil G., Di Poi C., et al. 2008. Feed demand behavior in sea bass juveniles: effects on individual specific growth rate variation and health (inter-individual and inter-group variation). Aquaculture 274:87-95 (IF: 1.925). 2. Di Poi C., et al. 2008. Effects of high food-demand fish removal in groups of juvenile Sea bass (Dicentrarchus labrax). Canadian Journal of Zoology 86:1015-1023 (IF: 1.254). 1. Di Poi C., et al. 2007. Behavioral and neurophysiological responses of European sea bass groups reared under food constraint. Physiology & Behavior 90:559-566 (IF: 3.295).

COMMUNICATIONS

Invited talks

2. Di Poi C. 2014. Sub-lethal effects of psychotropic drugs on aquatic organisms: linking behaviour to neurobiological correlates. Public seminar at Lakehead University in the framework of a job interview to a Canada Research Chair position (Thunder Bay, Canada). 1. Di Poi C. 2013. The Study of individual variability in feeding behaviour and its structuration: Examples in Fish and Cephalopods. French Society for the Study of Animal Behaviour (Dijon, France).

Conference talks

23. Di Poi C., Minguez L., Costil K., Lebel J.-M., Budzinsky H., Bureau R., Bidel F., Bellanger C., Cossu- Leguille C., Serpentini A., Halm M.-P. 2015. Pharm@ecotox: Impact écotoxicologique des résidus pharmaceutiques au niveau dulcicole et marin. SEFA (Bordeaux, France). 22. Bidel F., Bellanger C., Boulourd M., Darmaillacq A-S., Dickel L., Di Poi C., Jozet-Alves C., Olley R. 2015. Antidepressant exposure during early postembryonic development modulates brain plasticity, brain architecture and behaviours in cuttlefish (Sepia officinalis). PRIMO18 (Trondheim, Norvège). 21. Minguez L., Di Poi C., Costil K., Lebel J.-M., Budzinsky H., Bureau R., Bidel F., Bellanger C., Cossu- Leguille C., Serpentini A., Halm M.-P. 2015. Pharm@ecotox : Impact écotoxicologique des résidus pharmaceutiques au niveau dulcicole et marin. ECOBIM (Québec, Canada). 20. Bidel F., Di Poi C., Arini A., Basu N., Boulouard M., Darmaillacq A-S., Dickel L., Olley R., Jozet-Alves C., Bellanger C. 2014. Venlafaxine exposure during early postembryonic development is associated with neurochemical and behavioral changes in cuttlefish (Sepia officinalis). SETAC (Vancouver, Canada). 19. Bellanger C., Di Poi C., Bidel B., Jozet C., Dickel L., Boulouard M., Darmaillacq A.-S. 2014. Behavioural and cerebral changes occuring in the cuttlefish with perinatal exposure to antidepressant. 11th International Congress of Neuroethology (Sapporo, Japan). 18. Séguin A., Di Poi C., Evariste L., Mottier A., Serpentini A., Lebel J.-M., Costil K. 2014. Effets des antidépresseurs sur les jeunes stades de l’huître creuse, Crassostrea gigas. ECOBIM (Plouzané, France). 17. Di Poi C., Bidel F., Darmaillacq A.-S., Jozet C., Dickel L., Bellanger C. 2014. Brain and behaviour ontogenesis of the cuttlefish: impact of environmental pollution by pharmaceutical residues. COST FA1301 (Barcelona, Spain). 16. Bellanger C., Di Poi C. 2013. Etude écotoxicologique des psychotropes sur un céphalopode : approches comportementale et neurobiologique. Workshop « Les médicaments dans l’environnement » (Albi, France). 15. Di Poi C., Darmaillacq A.-S., Boulouard M., Bellanger C. 2013. Psychotropic drugs as behavioural disruptors in a non-target marine organism: What does the cuttlefish on Prozac tell us? Society for Experimental Biology's Annual Meeting (Valencia, Spain). 14. Di Poi C., Evariste L., Serpentini A., Halm-Lemeille M.-P., Lebel J.-M., Costil K. 2013. Toxicity of five antidepressant drugs on the embryo-larval development in the Pacific oyster, Crassostrea gigas. PRIMO17 Pollutant Responses in Marine Organisms (Faro, Portugal). 13. Di Poi C., Lacasse J., Amyot M., Rogers S., Aubin-Horth N. 2012. Marine and freshwater sticklebacks differ in behaviours and stress response: link with neurogenomic state. 7th International Conference on Stickleback Behavior and Evolution (Seattle, USA). 12. Di Poi C., Lacasse J., Rogers S., Aubin-Horth N. 2012. Behavioural and neurogenomic divergence between freshwater and marine threespine stickleback populations. 1st Joint Congress on Evolutionary Biology (Ottawa, Canada). 11. Di Poi C., Aubin-Horth N. 2011. The « Evolutionary History of temperament » told by the threespined stickleback. French Society for the Study of Animal Behaviour (Tours, France). 10. Di Poi C., Rogers S., Aubin-Horth N. 2010. Antipredator behavior in three-spined stickleback Gasterosteus aculeatus : Are freshwater fish more bold than their marine ancestor ? 5th Annual Canadian Society of Ecology and Evolution Meeting (Quebec City, Canada). 9. Di Poi C. 2010. À quel point le contexte social influence les comportements individuels et la réponse au stress chez les Vertébrés? Interlabs-IBIS (Quebec City, Canada). 8. Di Poi C., Atkinson S., Hoover-Miller A., Blundell G. 2010. The presence of the mother influences the stress response in Alaskan harbor seal pups Phoca vitulina. Annual Meeting of the Society for Integrative and Comparative Biology (Seattle, USA). 7. Di Poi C., Aubin-Horth N. 2009. Test du tempérament : en quoi les fonceurs et les timides sont-ils différents ? Un exemple chez les poissons. 1er Symposium des Études Postdoctorales à l’Université Laval (Quebec city, Canada). 6. Di Poi C., Attia J., Mathevon N., Beauchaud M. 2008. Individual recognition affects AVT expression in brain of European sea bass (Dicentrarchus labrax). 4th European Conference on Behavioral Biology (Dijon, France). Granted by the French Society for the study of Animal Behavior (SFECA). 5. Di Poi C., Beauchaud M., Schrama J., Begout M.-L., Attia J. 2008. Analysis of the self-feeding behavior in European sea bass juveniles using X-radiography. 9th day of Cytometry (Saint-Etienne, France). 4. Di Poi C., Beauchaud M., Schrama J., Begout M.-L., Attia J. 2008. Monitoring of the self-feeding behavior in European sea bass juveniles using X-radiography: Analysis of feed intake and feed efficiency. COST 867 « WellFish » (Krakow, Poland). 3. Di Poi C., Millot S., Attia J., Bégout M.-L., Beauchaud M. 2007. Social dynamic of group feeding in sea bass juveniles. COST 867 « WellFish » (Varese, Italy). 2. Di Poi C., Millot S., Beauchaud M., Dutto G., Covès D., Bégout M.-L., Attia J. 2006. Voluntary food demand in the sea bass, Dicentrarchus labrax: Behavioral and Neuro-physiological approaches. 3rd French Conference of Ichtyology (Paris, France). 1. Millot S., Di Poi C., Person-Le-Ruyet J., Sévère A., Pineau P., Bégout M.-L. 2006. Long- term analysis of the food demand in sea bass (Dicentrarchus labrax) populations reared in self-feeding conditions : Impact of the individually triggering activity on the group feeding. Oral contribution, 3rd French Conference of Ichtyology (Paris, France).

Posters

14. Atkinson S., Di Poi C., Hoover-Miller A., Blundell G. 2014. Free-ranging harbor seal pups experience maternal buffering of stress response. Alaska Marine Science Symposium (Anchorage, USA). 13. Bidel F., Bellanger C., Di Poi C., Jozet-Alves C. 2013. May marine pollution by psychotropic drugs impact the chance of survival in young cuttlefish (Sepia officinalis). Society for Experimental Biology's Annual Meeting (Valencia, Spain). 12. Boulouard M., Bellanger C., Darmaillacq A.-S., Dickel L., Di Poi C. 2013. Effects of perinatal exposure to waterborne fluoxetine on memory processing in the cuttlefish Sepia officinalis. 11e Colloque de la Société des Neurosciences (Lyon, France). 11. Di Poi C., Boulouard M., Darmaillacq A.-S., Bellanger C. 2013. Behavioural toxicity of the antidepressant fluoxetine in the common cuttlefish Sepia officinalis. PRIMO17 Pollutant Responses in Marine Organisms (Faro, Portugal). 10. Di Poi C., Le Pabic C., Darmaillacq A.-S., Koueta N., Bellanger C. 2012. Effects of waterborne fluoxetine on survival behaviors and physiological functions in young cuttlefish. Cephalopod International Advisory Council Symposium (Florianópolis, Brazil). 9. Bellanger C., Paniez E., Darmaillacq A.S., Boulouard M., Di Poi C. 2012. Fluoxetine exposure and development of fundamental behaviors and learning in a cephalopod (cuttlefish). 8th FENS Forum of Neuroscience (Barcelona, Spain). 8. Di Poi C., Paniez E., Darmaillacq A.S., Boulouard M., Bellanger C. 2012. Cuttlefish on Prozac: How depressing? Société Française pour l’Étude du Comportement Animal (Saint-Etienne, France). 7. Di Poi C., Aubin-Horth Nadia. 2010. Test of personality: Into the head of bold and shy three-spined sticklebacks. The Society for Behavioral Neuroendocrinology Annual Meeting (Toronto, Canada). 6. Di Poi C., Maniscalco J. M., Parker P., Atkinson S. 2009. A behavioral assessment of Steller sea lion (Eumotopias jubatus) pups exposed to hot branding: Do branded pups show greater signs of stress compared to unbranded pups? The 18th SMM Biennial Conference on the Biology of Marine Mammals (Québec City, Canada). 5. Di Poi C., Attia J., Bouchut C., Dutto G., Covès D., Beauchaud M. 2007. Behavioral and neurophysiological responses of European sea bass groups reared under food constraint. XXX International Ethological Conference (Halifax, Canada). 4. Di Poi C., Attia J., Bouchut C., Dutto G., Covès D., Beauchaud M. 2007. “Who leaves his place loses it”: Social knockout effect in captive groups of juvenile European sea bass. XXX International Ethological Conference (Halifax, Canada). 3. Di Poi C., Attia J., Beauchaud M. 2006. Behavioral and neurophysiological responses of European sea bass groups reared under controlled environment. French and Chinese young scientists meeting (Bejing, China). 2. Di Poi C., Attia J., Beauchaud M., Bouchut C., Covès D., Dutto G., Mathevon N. 2006. « Qui va à la chasse perd sa place! » Hierarchy and social group in the Mediterranean sea bass. Research day of the Doctoral School of Saint-Etienne (Saint-Etienne, France). 1. Di Poi C., Siebert U., Drouguet O., Fonfara S., Lepoint G., Das K. 2005. Stable isotopes and mercury in blood of Harbour seals (Phoca vitulina) caught on a German sandbank. 19th Conference of the European Cetacean Society (La Rochelle, France).

TEACHING EXPERIENCE

Table summarizing my experience as a sessional lecturer in French universities (Université Jean Monnet, Saint- Etienne and Université de Caen Basse-Normandie, Caen).

Courses Number of hours Degrees

Lectures Practical Laboratory classes classes Animal Physiology and 4 99 Neuroanatomy Haploid Genetic 52 Developmental Biology 25 Undergraduate Zoology 11 (Licence’s degree) Cell Biology 8 Biochemistry 8 Psychophysiology 18 12 Neuro-ethology 40 Graduate (Master’s degree) TOTAL 277 hours

TRAINING & SUPERVISORY EXPERIENCE

2005-2015 Supervision or co-supervision of Licence and Master’s students (9 Licences; 13 Masters)

OUTREACH ACTIVITIES

2009-2012-2013 Scientific animation at the « Fête de la science » (celebration day for sciences in France). 2010 Scientific representative for teenagers during the «Girls and Science» day (Quebec City, Canada). 2009 Science judge for the Alaska Tsunami Ocean Sciences Bowl (Seward, USA) 2006 Participation in the program « A Scientist in High-School ». The objective of the program is to introduce Sciences to children (12-15 years old).