Carole Di Poi Broussard, Ph.D. Current position: Postdoctoral Researcher in Marine Ecotoxicology & Ecophysiology Université de Caen Basse-Normandie CERMN UPRES EA 4258 - FR CNRS 3038 INC3M Phone: +33-629-668-274 UMR BOREA; MNHN, UPMC, UCBN, CNRS-7208, IRD-207 Email: [email protected] Esplanade de la Paix CS14032, F-14032 Caen, Cedex 5 RESEARCH FIELDS Invertebrate and Fish Ecology • Animal Behavior • Physiology and Neurobiology of Stress Aquatic Ecotoxicology • Physiology/Biochemistry of Detoxification and Oxidative Stress Regulation MODEL ORGANISMS Invertebrates: freshwater crustacean (e.g., daphnia), marine molluscs (e.g., bivalves, cephalopods) Vertebrates: fishes (e.g., sea bass, threespine stickleback), marine mammals (e.g., sea lion, harbor seal) TECHNICAL SKILLS Husbandry: rearing of marine/freshwater fishes and invertebrates, algae cultures. Behavioral analysis: field- and laboratory based studies (social and fitness-related behaviors, antipredator and predator behaviors). Organismal bioassays: embryotoxicity and metamorphosis bioassays, immobilization and reproduction bioassays, physiological responses (e.g., in ovo ventilatory rate in cuttlefish embryos). In vivo toxicity bioassays: biomarkers of cell oxidative stress and detoxification (catalase, GST), peroxidation of membranes (TBARS), neurotransmitter (monoamines, metabolites and receptors) and stress hormone (cortisol, corticosterone) quantification. In vitro toxicity bioassays: cell viability (MTT test), stability of cell membranes (Neutral Red Retention assay), immunotoxicity (phagocytic activity, ROS production). Technical skills: enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), High Performance Liquid Chromatography (HPLC), flow cytometry, RNA extraction and PCR, qRT-PCR. Image analysis: GIMP and ImageJ softwares, Jwatcher (ethograms). Bio-statistics: Statview, Statistica, StatXact, R soft OTHER RELEVANT SKILLS Management of research project, grant and funding proposal writing (in French and English) Teaching and mentorship Languages: English (fluent), French (native language) EDUCATION 2005-2008 Ph.D. in Neuro-Ethology of Fish ENES UMR 8195 Université Jean Monnet, Saint-Etienne (France) Funding: French Ministry of Higher Education and Research Supervisors: Pr. N. Mathevon and Dr. J. Attia Grade: Honors with oral congratulations 2004-2005 Master’s degree (2nd yr) in Physiology in Extreme Environments Université Claude Bernard, Lyon 1 (France) 2005 Training course in Cetology École Pratique des Hautes Études (EPHE), Montpellier (France) 2003-2004 Master’s degree (1st yr) in Marine Biology and Ecology Université de la Méditerranée, Marseilles (France) 2002-2003 License’s degree (3rd yr) in Marine Biology and Ecology Université de la Méditerranée, Marseilles (France) 2000-2002 License’s degree (2 yrs) in Life Sciences Université Joseph Fourier, Grenoble (France) RESEARCH EXPERIENCE January 2015-present Research fields: Marine Ecotoxicology, Ecophysiology CERMN UPRES EA 4258 - FR CNRS 3038 INC3M UMR BOREA; MNHN, UPMC, UCBN, CNRS-7208, IRD-207 Université de Caen Basse-Normandie, Caen (France) April 2012-March 2014 Research fields: Marine Ecotoxicology, Behavioral Biology, Ecophysiology GMPc EA4259 UMR BOREA; MNHN, UPMC, UCBN, CNRS-7208, IRD-207 Université de Caen Basse-Normandie, Caen (France) Dec. 2010-Dec. 2011 Research field: Behavioral Ecotoxicology, Ecophysiology GMPc EA4259 Université de Caen Basse-Normandie, Caen (France) Oct. 2009-Nov. 2010 Research field: Genomics of Behavior Nadia Aubin-Horth’s Lab Institute of Integrative and Systems Biology Laval University, Quebec City (Canada) Nov. 2008-Sept. 2009 Research fields: Behavioral Ecology, Endocrinology Shannon Atkinson’s Lab School of Fisheries and Ocean Sciences University of Alaska Fairbanks, Juneau (USA) FACULTY APPOINTMENTS Sept. 2005-Sept. 2008 Sessional lecturer (242 hrs of teaching in Biology) Université Jean Monnet, Saint-Etienne (France) Sept. 2011-June 2012 Sessional lecturer (30 hrs of teaching in Psychophysiology) Université de Caen Basse-Normandie, Caen (France) RESEARCH FUNDING, GRANTS & AWARDS 2015-2016 DARM project (Pharmaceuticals, Personal Care Products and ecotoxicology in marine bivalves) funded by the Région Basse- Normandie (principal investigator: Dr. M.-P. Halm UCBN). (Post-doc salary for 15 months) 2011-2015 Pharm@Ecotox project (Pharmaceutical residues and marine ecotoxicology) funded by the Agence Nationale de la Recherche ANR (principal investigator: Dr. M.-P. Halm UCBN). (Post-doc salary for 24 months) 2010-2011 Postdoctoral Research Fellowship (Antidepressant residues and marine ecotoxicology in cephalopods) funded by the Université de Caen Basse- Normandie - 32.000 euros (principal investigator) 2010 Travel grant of the Canadian Society of Zoology - US$500 2009-2010 Government of Canada Postdoctoral Research Fellowship (PDRF) (Behavioral and molecular divergence in threespine stickleback) funded by the Government of Canada - CAD$32.000 (principal investigator) 2008-2009 Postdoctoral Fellowship (Maternal buffering of stress response in pinniped pups) funded by the Fyssen Foundation - 25.000 euros (principal investigator) 2008 Travel grant of the French Society for the Study of Animal Behavior (SFECA) - 250 euros 2007 Award for Ph.D. Excellence, Women in Sciences Program from L’Oréal France-UNESCO-French Academy of Science - 10.000 euros OTHER RESEARCH ACTIVITIES 2013: Participation to the COST FA1301 “A network for improvement of cephalopod welfare and husbandry in research, aquaculture and fisheries (CephsInAction)”. 2011-2013: Student and Postdoc representative at the GMPc lab council (Université de Caen Basse-Normandie, Caen, France) 2010: Organization of the 5th Annual Meeting of the Canadian Society for Ecology and Evolution (Quebec City, Canada) 2005-2008: Participation to the COST 867 WELLFISH “Welfare of Fish in European Aquaculture” Scientific Journal Reviewer: Biology Letters, Behavioural Brain Research, Aquatic Toxicology, etc. Member to scientific societies: French Society for the Study of Animal Behavior (SFECA), Association for the Research in Toxicology (ARET), Society for Experimental Biology (SEB). LIST OF PUBLICATIONS production: 1.6 articles/year Forthcoming articles 5. Bidel F., Di Poi C. et al. Changes in cuttlefish brain following early postembryonic exposure to Venlafaxine (antidepressant): Neurodevelopmental and behavioral outcomes. In preparation. 4. Di Poi C., Halm-Lemeille M-.P., Deft P., Budzinski H., Darmaillacq A.-S., Bellanger C. Acute toxicity of the antidepressant fluoxetine in cuttlefish embryos. In preparation. 3. Di Poi C., Bélanger D., Amyot M., Rogers S., Aubin-Horth N. Evolution of four regulatory molecular networks associated with behaviour divergence in sticklebacks. Submitted (Current Biology). 2. Bidel F., Le Pabic C., Imarazene B., Koueta N., Di Poi C. et al. Biochemical and predatory behavior changes in juvenile cuttlefish (Sepia officinalis) following exposure to environmental concentrations of Venlafaxine (antidepressant). Submitted (Aquatic Toxicology; special issue: Behavioural Aquatic Toxicology). 1. Di Poi C., Lacasse J., Rogers S., Aubin-Horth N. Habitat-associated evolution of stress reactivity in sticklebacks. Submitted (Biology Letters). Published articles 16. Bidel F., Di Poi C., Imarazene B., Koueta N., Budzinski H., Van Delft P., Bellanger B., Jozet-Alves C. In press. Pre-hatching fluoxetine-induced neurochemical, neurodevelopmental, and immunological changes in newly hatched cuttlefish. Environmental Science and Pollution Research. 15. Di Poi C., Hoover-Miller A., Blundell G., Atkinson S. 2015. Maternal buffering of stress response in free- ranging Pacific harbor seal pups in Alaska. Marine Mammal Science 31(3):1098-1117 (IF: 2.128). 14. Di Poi C., Lacasse J., Rogers S., Aubin-Horth N. 2014. Extensive behavioural divergence following colonisation of the freshwater environment in threespine sticklebacks. PLOS One 9(6):e98980 DOI: 10.1371/journal.pone.0098980 (IF: 4.09). 13. Di Poi C., Evariste L., Séguin A., Mottier A., Pedelucq J., Lebel J.-M., Serpentini A., Budzinski H., Costil K. 2014. Sub-chronic exposure to fluoxetine in juvenile oysters (Crassostrea gigas): uptake and biological effects. Environmental Science and Pollution Research DOI 10.1007/s11356-014-3702-1 (IF: 2.618). 12. Minguez L., Di Poi C., Farcy E., Ballandonne C., Benchouala A., Bojic C., Cossu-Leguille C., Costil K., Serpentini A., Lebel JM., Halm-Lemeille M.-P. 2014. Comparison of the sensitivity of seven marine and freshwater bioassays as regards antidepressant toxicity assessment. Ecotoxicology 23(9):1744-1754 (IF: 2.773). 11. Di Poi C., Bidel F., Dickel L., Bellanger C. 2014. Cryptic and biochemical responses in young cuttlefish Sepia officinalis exposed to environmentally relevant concentrations of fluoxetine. Aquatic Toxicology 151:36-45 (IF: 3.761). 10. Di Poi C., Bellanger C. 2014. Response to commentary on “Are some invertebrates exquisitely sensitive to the human pharmaceutical fluoxetine?”. Aquatic Toxicology 146:261-263 (IF: 3.730). 9. Di Poi C., Evariste L., Serpentini A., Halm-Lemeille M.-P., Lebel J.-M., Costil K. 2014. Toxicity of five antidepressant drugs on the embryo-larval development and metamorphosis in the Pacific oyster, Crassostrea gigas. Environmental Science and Pollution Research 21(23):13302-13314 (IF: 2.618). 8. Di Poi C., Darmaillacq A.-S., Dickel L., Boulouard M., Bellanger C. 2013. Effects of perinatal exposure to waterborne fluoxetine on memory processing in the cuttlefish