PTPN2 Antibody Cat. No.: 30-061
PTPN2 Antibody
Antibody used in WB on Human Jurkat cells at 2.5 ug/ml.
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Dog, Human, Mouse, Rat
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human PTPN2.
TESTED APPLICATIONS: ELISA, WB
PTPN2 antibody can be used for detection of PTPN2 by ELISA at 1:312500. PTPN2 APPLICATIONS: antibody can be used for detection of PTPN2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
POSITIVE CONTROL: 1) Cat. No. 1205 - Jurkat Cell Lysate
PREDICTED MOLECULAR 39 kDa, 49 kDa, 45 kDa WEIGHT:
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PURIFICATION: Antibody is purified by protein A chromatography method.
CLONALITY: Polyclonal
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.
CONCENTRATION: batch dependent
For short periods of storage (days) store at 4˚C. For longer periods of storage, store STORAGE CONDITIONS: PTPN2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Additional Info
OFFICIAL SYMBOL: PTPN2
ALTERNATE NAMES: PTPN2, PTPT, TC-PTP, TCELLPTP, TCPTP, PTN2
ACCESSION NO.: NP_536348
PROTEIN GI NO.: 18104982
GENE ID: 5771
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
PTPN2 is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling.The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic BACKGROUND: motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Three alternatively spliced variants of this gene, which encode isoforms differing at their extreme C-termini, have been described. The different C-termini are thought to determine the substrate specificity, as well as the cellular localization of the isoforms. Two highly related but distinctly processed pseudogenes that localize to distinct chromosomes have been reported.
REFERENCES: 1) Shimizu, T., (2004) Exp. Hematol. 32 (11), 1057-1063.
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