PTPN2 Antibody Cat. No.: 30-061

PTPN2 Antibody

Antibody used in WB on Human Jurkat cells at 2.5 ug/ml.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Dog, Human, Mouse, Rat

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human PTPN2.

TESTED APPLICATIONS: ELISA, WB

PTPN2 antibody can be used for detection of PTPN2 by ELISA at 1:312500. PTPN2 APPLICATIONS: antibody can be used for detection of PTPN2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

POSITIVE CONTROL: 1) Cat. No. 1205 - Jurkat Cell Lysate

PREDICTED MOLECULAR 39 kDa, 49 kDa, 45 kDa WEIGHT:

September 30, 2021 1 https://www.prosci-inc.com/ptpn2-antibody-30-061.html Properties

PURIFICATION: Antibody is purified by A chromatography method.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.

CONCENTRATION: batch dependent

For short periods of storage (days) store at 4˚C. For longer periods of storage, store STORAGE CONDITIONS: PTPN2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Additional Info

OFFICIAL SYMBOL: PTPN2

ALTERNATE NAMES: PTPN2, PTPT, TC-PTP, TCELLPTP, TCPTP, PTN2

ACCESSION NO.: NP_536348

PROTEIN GI NO.: 18104982

GENE ID: 5771

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

PTPN2 is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated .The protein encoded by this is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic BACKGROUND: motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Three alternatively spliced variants of this gene, which encode isoforms differing at their extreme C-termini, have been described. The different C-termini are thought to determine the substrate specificity, as well as the cellular localization of the isoforms. Two highly related but distinctly processed that localize to distinct have been reported.

REFERENCES: 1) Shimizu, T., (2004) Exp. Hematol. 32 (11), 1057-1063.

September 30, 2021 2 https://www.prosci-inc.com/ptpn2-antibody-30-061.html ANTIBODIES FOR RESEARCH USE ONLY.

For additional information, visit ProSci's Terms & Conditions Page.

September 30, 2021 3 https://www.prosci-inc.com/ptpn2-antibody-30-061.html