Research Article

Haryana Vet. (Dec., 2019) 58(2), 166-169 Research Article

ABSTRACT Fig 2: Transmission electron photomicrograph of monocyte of dog Present study comprised of 72 crossbred cows (group I= 60 endometritic and group II=12 healthy) at 30±2days postpartum. The Fig. 1. Lateral view of ribs (13 numbers) of left side of adult female showing heterochromatin (a), euchromatin (b), cytoplasmic process (c), polymorphonuclear neutrophils (PMN) cell coun  Vacuole and nuclear membrane. Bule bull (Boselaphus tragocamelus) Uranyl acetate and lead citrate × 25500

Fig. 1. Histogram depicting frequency distribution of animal right score of respondents

Figure 1: Cyclic conditions for PCR profiling for detection of Salmonella genes

Fig. 1. Semilogarithmic plot of plasma concentration time profile of ASSOCIATION OF SEMEN TRAITS IN amoxicillin and cloxacillin following single dose (10 mg/kg) i.v. and Marinelli, L., Adamelli, S., Normando, S. and Bono, G. i.m. administration in sheep (n=4) (2007). Quality of life of the pet dog: Influence of CONSECUTIVE EJACULATES WITH FSH-β GENE owner and dog’s characteristics. Appl. Anim. Behav. POLYMORPHISM IN HOLSTEIN-FRIESIAN Sci. 108(1- 1 2 CROSSBRED BULLS FROM VIJAY KADAM, ABH

trus synchronizathod that synchronizes ovulations is Corresponding author: [email protected] named briefly as “Ovsynch” (Pursley et al., 1995). The Clinical Article study was aimed to evaluate the efficacy of different methods of estrus sync

Haryana Vet. (Dec., 2019) 58(2), 166-169 Research Article Haryana Vet. (June, 2020) 59(1), 84-87 Research Article complete diet was dried and converted to powder form and curd consumption in urban areas (893.30 g and 184.16 ml) stored for analysis. The feed samples were analyzed for of Mansa district is higher (P0.05) than urban areas COMPARATIVE EFFICACY OF SYNCHRONIZATION PROTOCOLS FOR IMPROVING NUTRITIONAL AND MANAGEMENTAL PRACTICES FOLLOWED BY DOG OWNERS IN proximate composition (moisture, crude protein (CP), (533.30 g and 143.33 ml) of district. Whereas, no FERTILITY IN POSTPARTUM CROSSBRED DAIRY COWS BARNALA AND MANSA DISTRICT OF ether extract (EE), total ash (TA), acid insoluble ash (AIA), significant (P0.05) difference in feeding of milk and RATNAPARKHI A.R., DESHMUKH S.G.*, BIRADE H.S., KALE V.B., HARKAL S.B. and JADHAO A.D. ANKITA TIWARI*, A.P.S. SETHI, UDEYBIR SINGH, M. WADHWA, M. CHATLI and NEERAJ KASHYAP crude fiber (CF), minerals (calcium and phosphorus), sweets had been observed in urban areas of both districts. Department of Animal Reproduction, Gynaecology and Obstetrics, Department of Animal Nutrition physicochemical properties (pH, free fatty acids and Feeding of chapatti, rice, dal and vegetables by dog owners Post Graduate Institute of Veterinary and Animal Sciences, Akola-444104, India peroxide value) and microbiological counts (total plate Corresponding author: [email protected] Guru Angad Dev Veterinary and Animal Sciences University, -141004, India to their dogs showed non-significant (P0.05) differences count and coliform count). Received: 20.03.2019; Accepted: 13.05.2019 Received: 31.07.2019; Accepted: 01.02.2020 in both the districts. As far as the consumption of non- 182 RESULTS AND DISCUSSION vegetarian feed stuffs were concerned, it was observed that ABSTRACT ABSTRACT The perusal of data (Table 1) with regards to milk, quantity of eggs, meat and bone offered to dogs, shows no The objective of present research work was to study the efficacy of two different synchronization protocols for improving the fertility in A survey was conducted to study nutritional and managemental practices followed by dog owners in Barnala and Mansa districts of Punjab milk product, sweet and curd offered to rural dogs showed postpartum crossbred cows. A totah protocol induces better estrus response and first service conception rate in postpartum cows. state. For this, sixty (30 from urban and 30 from rural) and sixty two (30 from urban and 32 from rural) dog owners in Barnala and Mansa districts, significant (P0.05) difference between two districts. consumption of milk product was more (P 0.05) in rural Keywords: Conception rate, Double PG, Ovsynch protocol respectively, were surveyed. Percentage usage of food items fed to dogs such as milk, milk product, curd, dal, vegetables, meat, bone and egg were  After scrutinizing the data of urban and rural dogs more in Mansa than Barnala district. In urban Barnala, the quantity of dal, meat, bone, eggs offered to the pet dogs was higher (P<0.05) whereas in areas (815.60 g) of Mansa in contrast to rural areas (491.70 g) (Table 1) of Mansa district, it was observed that the food items urban Mansa, the quantity of the curd and chapatti offered to the pet dogs was higher (P<0.05) than that offered in the rural areas of Barnala and of Barnala district while no significant (P0.05) difference Mansa. Homemade food was preferred (72.6%) in Barnala district; however, mixed food (52.7%) was the choice of Mansa district. Based on the data such as milk, milk product, sweet, shows non-significant between Barnala and Mansa district was noticed in available, it is clearly indicated that feeding practices followed by the dog owners in rural and urban areas of Barnala and Mansa districts of Punjab (P0.05) difference in urban and rural areas of this district closely followed the trend of food consumed by the dog owners and no scientific feeding schedules are being followed. consumption of milk, sweet and curd. Average quantity of but curd intake is more (P0.05) in urban areas (184.20 g) Keywords: Barnala, Dogs, Feeding practices, Mansa, Survey dal and vegetables offered to dog in rural areas (82.81 g and 54.38 g) of Mansa district was higher (P0.05) compared to in contrast to rural areas (103.10 g). Higher (P0.05) The estrus synchronizathod that synchronizes The dogs being first animal to be tamed and their Survey proforma rural areas of Barnala (14.00 g and 22.83 g) district. No consumption of average number of chapattis by dogs was ovulations is named briefly as “Ovsynch” (Pursley et al., long association with human led to acclimatization of dogs A sample survey proforma was designed and observed in urban areas (7.00) as compared to rural areas 1995). The study was aimed to evaluate the efficacy of significant (P0.05) difference in feeding of chapatti and to survive on human foods. Dogs share some specific pretested by conducting survey after thorough (5.16). Whereas, the quantity of dal offered by dog owners different methods of estrus synchronisation protocols in rice were observed in rural areas of both the districts. As far carnivorous traits with cats as both lack salivary amylase, deliberations after which a well designed questionnaire as non-vegetarian diet, it was noticed that bone, egg and to their dog was more (P0.05) in rural areas (82.81g) in crossbred postpartum cows. have a short gastrointestinal tract and are unable to was prepared. This survey proforma includes the queries meat offered to dogs was more (P0.05) in rural areas contrary to urban areas (45.00g) of Mansa district. For non MATERIAL AND METHODS synthesize vitamin D. A human and pet relationship exists regarding the feeding practices followed by the dog (33.59 g, 1.94, 112.50 g) of Mansa district in comparison to vegetarian diet, it was noticed that more (P0.05) bones were A total of 20 healthy crossbred cows at 60-90 days parallel and the pet owners want to provide their dogs and owners such as kind of feed, quantity and number of times rural areas (6.67 g, 0.27 and 13.33 g) Barnala district. offered by dogs owners in rural areas (33.59g) compared to cats with alternative foods, such as commercially in a day feed offered to dogs which include both vegetarian Analysis of the data of urban dogs (Table 1) of Barnala urban (15.83g) areas. available, “natural”, raw food and vegetarian diets as they and non-vegetarian feedstuffs. Either these are fed raw or and Mansa districts indicates that the milk product and The perusal of the data (Table 1) of urban dogs and are considered as family members (Michel, 2006). In cooked. Besides this, some managemental and health Table 1 Punjab, total dog population is 4,70,558, out of which in aspect like cleanliness practices, vaccinations, deworming Feeding Practices followed by dog owners in Barnala and Mansa Districts rural areas is 3,89,831 and in urban areas is 80,727 (Livestock were also made part of this survey proforma. The survey Census, 2012). Pet dog owners usually feed their dog as was conducted in Barnala and Mansa districts of Punjab. Mansa Barnala per their convenience and the food items mainly include the Selection of dog owners Variable Urban (30) Rural (32) Urban (30) Rural (30) leftovers of homemade cooked food which are nutritionally A total of 122 dog owners were surveyed from Milk and milk products imbalanced with respect to protein, energy and minerals Barnala and Mansa district of Punjab. The survey was Milk (ml) 856.70 ± 100.90 871.90 ± 85.52 783.30 ± 99.38 878.30 ± 85.03 Milk product (g) 893.30A ± 140.80 815.60a ± 132.20 533.30B ± 74.60 491.70b ± 79.04 especially calcium and phosphorus (Shakhar et al., 2010). conducted by visiting dog owner’s houses, district polyclinics Curd (ml) 184.20xA ± 32.11 103.10y ± 21.88 143.33B ± 30.93 193.30 ± 49.64 The most common deficiencies encountered are in calcium and private veterinary clinics. Data from both the districts Sweet (g) 8.67 ± 2.71 4.06 ± 2.28 12.33 ± 3.06 13.00 ± 4.74 levels, micronutrients such as zinc, copper, choline, vitamin were uploaded and coded for analysis. Statistical analyses Cereals and pulses D and vitamin E levels (Stockman et al., 2013). Currently, were performed by use of SAS (2011) software. Chapatti (#) 7.00x ± 0.59 5.16y ± 0.42 7.23 ± 0.63 6.37 ± 0.61 no data have been available regarding the nutritional and Source of food sample Dal (g) 45.00y ± 8.76 82.81xa ± 13.58 44.37x ± 14.04 14.00yb ± 5.31 managemental practices adopted by dog owners in The different dog food ingredients such as milk, Rice (g) 13.33 ± 6.31 7.81 ± 4.55 10.00 ± 4.42 10.00 ± 5.57 Barnala and Mansa districts of Punjab. Therefore, this study chapatti, pulses, fruits, meat, eggs, vegetables etc. were Fruits and vegetables was planned with the objective to determine the nutritional a b collected from the owner at his/her location in an insulated Vegetables (g) 39.33 ± 8.44 54.38 ± 10.10 19.83 ± 5.98 22.83 ± 9.13 and managemental practices adopted by dog owners. Non- vegetarian container to maintain the freshness of food and were Meat (g) 55.00 ± 18.93 112.50a ± 25.70 54.67x ± 13.82 13.33yb ± 6.31 MATERIALS AND METHODS brought to the Department of Animal Nutrition. A total of y xa x yb Bone (g) 15.83 ± 4.87 33.59 ± 7.58 20.33 ± 4.51 6.67 ± 3.16 15 samples were collected from the owners of Barnala and a x yb To fulfill the objective of the study, the present Egg (QT) 1.47 ± 0.27 1.94 ± 0.23 0.90 ± 0.19 0.27 ± 0.12 investigation was carried out to study the different feeding Mansa districts of Punjab. Value in parenthesis represents number of respondents; Figures with different superscripts in a row differ significantly (P0.05) practices adopted by dog owners in Barnala and Mansa Analysis of Dog food samples l x,y depicts the significant difference in variable between urban and rural areas of same district. district. A complete diet was prepared according to their l A,B depicts the significant difference in variable between urban areas of both district. Corresponding author: [email protected] *Corresponding author: [email protected] proportion in the diet of dog as offered by their owner. The l a,b depicts the significant difference in variable between rural areas of both district.

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CONTRIBUTORS MAY NOTE CONTRIBUTORS MAY NOTE l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians THE HARYANA VETERINARIAN THE HARYANA VETERINARIAN l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. engaged in Veterinary Profession. l Please follow strictly the format of 'The Haryana Veterinarian' for manuscriptEditors/Editorial Board Members are highly thankful to Editors/Editorial Board Members are highly thankful to l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript writing/submission. writing/submission. all the distinguished referees who helped us in the all the distinguished referees who helped us in the l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. evaluation of articles. We request them to continue to evaluation of articles. We request them to continue to Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. extend their co-operation and be prompt in future to give extend their co-operation and be prompt in future to give l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick response. their valuable comments on the articles for timely their valuable comments on the articles for timely l Please mention your article reference number in all correspondence for a quick response. l We solicit your co-operation. publication of the journal. publication of the journal. l We solicit your co-operation. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Sciences, LUVAS, Hisar-125004. Editors Editors

CONTRIBUTORS MAY NOTE THE HARYANA VETERINARIAN l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians Editors/Editorial Board Members are highly thankful to THE HARYANA VETERINARIAN engaged in Veterinary Profession. CONTRIBUTORS MAY NOTE all the distinguished referees who helped us in the THE HARYANA VETERINARIAN l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript evaluation of articles. We request them to continue to Editors/Editorial Board Members are highly thankful to writing/submission. l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians Editors/Editorial Board Members are highly thankful to l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of engaged in Veterinary Profession. extend their co-operation and be prompt in future to give all the distinguished referees who helped us in the Veterinary Sciences, along with each article. l all the distinguished referees who helped us in the Please follow strictly the format of 'The Haryana Veterinarian' for manuscript their valuable comments on the articles for timely evaluation of articles. We request them to continue to l After revision, please return the revised manuscript and rebuttal at the earliest. writing/submission. evaluation of articles. We request them to continue to l l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of publication of the journal. extend their co-operation and be prompt in future to give Please mention your article reference number in all correspondence for a quick extend their co-operation and be prompt in future to give response. Veterinary Sciences, along with each article. their valuable comments on the articles for timely l We solicit your co-operation. l After revision, please return the revised manuscript and rebuttal at the earliest. their valuable comments on the articles for timely publication of the journal. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, l Please mention your article reference number in all correspondence for a quick publication of the journal. Department of Veterinary Public Health and Epidemiology, College of Veterinary response. Sciences, LUVAS, Hisar-125004. l We solicit your co-operation.

Editors l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Editors THE HARYANA VETERINARIAN CONTRIBUTORS MAY NOTE

Editors/Editorial Board Members are highly thankful to l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. THE HARYANA VETERINARIAN all the distinguished referees who helped us in the CONTRIBUTORS MAY NOTE l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript Editors/Editorial Board Members are highly thankful to evaluation of articles. We request them to continue to writing/submission. l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. extend their co-operation and be prompt in future to give l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of all the distinguished referees who helped us in the Veterinary Sciences, along with each article. evaluation of articles. We request them to continue to l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript their valuable comments on the articles for timely l After revision, please return the revised manuscript and rebuttal at the earliest. writing/submission. extend their co-operation and be prompt in future to give l publication of the journal. l Please mention your article reference number in all correspondence for a quick Please pay processing fee of Rs. 1000/- online in the account of Dean, College of response. their valuable comments on the articles for timely Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. l We solicit your co-operation. publication of the journal. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, l Please mention your article reference number in all correspondence for a quick Department of Veterinary Public Health and Epidemiology, College of Veterinary response. Sciences, LUVAS, Hisar-125004. l We solicit your co-operation.

Editors l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Editors THE HARYANA VETERINARIAN Editors/Editorial Board Members are highly thankful to all the distinguished referees who helped us in the evaluation of articles. We request them to continue to extend their co-operation and be prompt in future to give their valuable comments on the articles for timely publication of the journal.

CONTRIBUTORS MAY NOTE l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript writing/submission. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick response. l We solicit your co-operation. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Editors

15 22 Research Article

Haryana Vet. (Dec., 2019) 58(2), 166-169 Research Article

ABSTRACT Fig 2: Transmission electron photomicrograph of monocyte of dog Present study comprised of 72 crossbred cows (group I= 60 endometritic and group II=12 healthy) at 30±2days postpartum. The Fig. 1. Lateral view of ribs (13 numbers) of left side of adult female showing heterochromatin (a), euchromatin (b), cytoplasmic process (c), polymorphonuclear neutrophils (PMN) cell coun  Vacuole and nuclear membrane. Bule bull (Boselaphus tragocamelus) Uranyl acetate and lead citrate × 25500

Fig. 1. Histogram depicting frequency distribution of animal right score of respondents

Figure 1: Cyclic conditions for PCR profiling for detection of Salmonella genes

Fig. 1. Semilogarithmic plot of plasma concentration time profile of ASSOCIATION OF SEMEN TRAITS IN amoxicillin and cloxacillin following single dose (10 mg/kg) i.v. and Marinelli, L., Adamelli, S., Normando, S. and Bono, G. i.m. administration in sheep (n=4) (2007). Quality of life of the pet dog: Influence of CONSECUTIVE EJACULATES WITH FSH-β GENE owner and dog’s characteristics. Appl. Anim. Behav. POLYMORPHISM IN HOLSTEIN-FRIESIAN Sci. 108(1- 1 2 CROSSBRED BULLS FROM INDIA VIJAY KADAM, ABH

trus synchronizathod that synchronizes ovulations is Corresponding author: [email protected] named briefly as “Ovsynch” (Pursley et al., 1995). The Clinical Article study was aimed to evaluate the efficacy of different methods of estrus sync

Haryana Vet. (Dec., 2019) 58(2), 166-169 Research Article Haryana Vet. (June, 2020) 59(1), 84-87 Research Article complete diet was dried and converted to powder form and curd consumption in urban areas (893.30 g and 184.16 ml) stored for analysis. The feed samples were analyzed for of Mansa district is higher (P0.05) than urban areas COMPARATIVE EFFICACY OF SYNCHRONIZATION PROTOCOLS FOR IMPROVING NUTRITIONAL AND MANAGEMENTAL PRACTICES FOLLOWED BY DOG OWNERS IN proximate composition (moisture, crude protein (CP), (533.30 g and 143.33 ml) of Barnala district. Whereas, no FERTILITY IN POSTPARTUM CROSSBRED DAIRY COWS BARNALA AND MANSA DISTRICT OF PUNJAB ether extract (EE), total ash (TA), acid insoluble ash (AIA), significant (P0.05) difference in feeding of milk and RATNAPARKHI A.R., DESHMUKH S.G.*, BIRADE H.S., KALE V.B., HARKAL S.B. and JADHAO A.D. ANKITA TIWARI*, A.P.S. SETHI, UDEYBIR SINGH, M. WADHWA, M. CHATLI and NEERAJ KASHYAP crude fiber (CF), minerals (calcium and phosphorus), sweets had been observed in urban areas of both districts. Department of Animal Reproduction, Gynaecology and Obstetrics, Department of Animal Nutrition physicochemical properties (pH, free fatty acids and Feeding of chapatti, rice, dal and vegetables by dog owners Post Graduate Institute of Veterinary and Animal Sciences, Akola-444104, India peroxide value) and microbiological counts (total plate Corresponding author: [email protected] Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, India to their dogs showed non-significant (P0.05) differences count and coliform count). Received: 20.03.2019; Accepted: 13.05.2019 Received: 31.07.2019; Accepted: 01.02.2020 in both the districts. As far as the consumption of non- 182 RESULTS AND DISCUSSION vegetarian feed stuffs were concerned, it was observed that ABSTRACT ABSTRACT The perusal of data (Table 1) with regards to milk, quantity of eggs, meat and bone offered to dogs, shows no The objective of present research work was to study the efficacy of two different synchronization protocols for improving the fertility in A survey was conducted to study nutritional and managemental practices followed by dog owners in Barnala and Mansa districts of Punjab milk product, sweet and curd offered to rural dogs showed postpartum crossbred cows. A totah protocol induces better estrus response and first service conception rate in postpartum cows. state. For this, sixty (30 from urban and 30 from rural) and sixty two (30 from urban and 32 from rural) dog owners in Barnala and Mansa districts, significant (P0.05) difference between two districts. consumption of milk product was more (P 0.05) in rural Keywords: Conception rate, Double PG, Ovsynch protocol respectively, were surveyed. Percentage usage of food items fed to dogs such as milk, milk product, curd, dal, vegetables, meat, bone and egg were  After scrutinizing the data of urban and rural dogs more in Mansa than Barnala district. In urban Barnala, the quantity of dal, meat, bone, eggs offered to the pet dogs was higher (P<0.05) whereas in areas (815.60 g) of Mansa in contrast to rural areas (491.70 g) (Table 1) of Mansa district, it was observed that the food items urban Mansa, the quantity of the curd and chapatti offered to the pet dogs was higher (P<0.05) than that offered in the rural areas of Barnala and of Barnala district while no significant (P0.05) difference Mansa. Homemade food was preferred (72.6%) in Barnala district; however, mixed food (52.7%) was the choice of Mansa district. Based on the data such as milk, milk product, sweet, shows non-significant between Barnala and Mansa district was noticed in available, it is clearly indicated that feeding practices followed by the dog owners in rural and urban areas of Barnala and Mansa districts of Punjab (P0.05) difference in urban and rural areas of this district closely followed the trend of food consumed by the dog owners and no scientific feeding schedules are being followed. consumption of milk, sweet and curd. Average quantity of but curd intake is more (P0.05) in urban areas (184.20 g) Keywords: Barnala, Dogs, Feeding practices, Mansa, Survey dal and vegetables offered to dog in rural areas (82.81 g and 54.38 g) of Mansa district was higher (P0.05) compared to in contrast to rural areas (103.10 g). Higher (P0.05) The estrus synchronizathod that synchronizes The dogs being first animal to be tamed and their Survey proforma rural areas of Barnala (14.00 g and 22.83 g) district. No consumption of average number of chapattis by dogs was ovulations is named briefly as “Ovsynch” (Pursley et al., long association with human led to acclimatization of dogs A sample survey proforma was designed and observed in urban areas (7.00) as compared to rural areas 1995). The study was aimed to evaluate the efficacy of significant (P0.05) difference in feeding of chapatti and to survive on human foods. Dogs share some specific pretested by conducting survey after thorough (5.16). Whereas, the quantity of dal offered by dog owners different methods of estrus synchronisation protocols in rice were observed in rural areas of both the districts. As far carnivorous traits with cats as both lack salivary amylase, deliberations after which a well designed questionnaire as non-vegetarian diet, it was noticed that bone, egg and to their dog was more (P0.05) in rural areas (82.81g) in crossbred postpartum cows. have a short gastrointestinal tract and are unable to was prepared. This survey proforma includes the queries meat offered to dogs was more (P0.05) in rural areas contrary to urban areas (45.00g) of Mansa district. For non MATERIAL AND METHODS synthesize vitamin D. A human and pet relationship exists regarding the feeding practices followed by the dog (33.59 g, 1.94, 112.50 g) of Mansa district in comparison to vegetarian diet, it was noticed that more (P0.05) bones were A total of 20 healthy crossbred cows at 60-90 days parallel and the pet owners want to provide their dogs and owners such as kind of feed, quantity and number of times rural areas (6.67 g, 0.27 and 13.33 g) Barnala district. offered by dogs owners in rural areas (33.59g) compared to cats with alternative foods, such as commercially in a day feed offered to dogs which include both vegetarian Analysis of the data of urban dogs (Table 1) of Barnala urban (15.83g) areas. available, “natural”, raw food and vegetarian diets as they and non-vegetarian feedstuffs. Either these are fed raw or and Mansa districts indicates that the milk product and The perusal of the data (Table 1) of urban dogs and are considered as family members (Michel, 2006). In cooked. Besides this, some managemental and health Table 1 Punjab, total dog population is 4,70,558, out of which in aspect like cleanliness practices, vaccinations, deworming Feeding Practices followed by dog owners in Barnala and Mansa Districts rural areas is 3,89,831 and in urban areas is 80,727 (Livestock were also made part of this survey proforma. The survey Census, 2012). Pet dog owners usually feed their dog as was conducted in Barnala and Mansa districts of Punjab. Mansa Barnala per their convenience and the food items mainly include the Selection of dog owners Variable Urban (30) Rural (32) Urban (30) Rural (30) leftovers of homemade cooked food which are nutritionally A total of 122 dog owners were surveyed from Milk and milk products imbalanced with respect to protein, energy and minerals Barnala and Mansa district of Punjab. The survey was Milk (ml) 856.70 ± 100.90 871.90 ± 85.52 783.30 ± 99.38 878.30 ± 85.03 Milk product (g) 893.30A ± 140.80 815.60a ± 132.20 533.30B ± 74.60 491.70b ± 79.04 especially calcium and phosphorus (Shakhar et al., 2010). conducted by visiting dog owner’s houses, district polyclinics Curd (ml) 184.20xA ± 32.11 103.10y ± 21.88 143.33B ± 30.93 193.30 ± 49.64 The most common deficiencies encountered are in calcium and private veterinary clinics. Data from both the districts Sweet (g) 8.67 ± 2.71 4.06 ± 2.28 12.33 ± 3.06 13.00 ± 4.74 levels, micronutrients such as zinc, copper, choline, vitamin were uploaded and coded for analysis. Statistical analyses Cereals and pulses D and vitamin E levels (Stockman et al., 2013). Currently, were performed by use of SAS (2011) software. Chapatti (#) 7.00x ± 0.59 5.16y ± 0.42 7.23 ± 0.63 6.37 ± 0.61 no data have been available regarding the nutritional and Source of food sample Dal (g) 45.00y ± 8.76 82.81xa ± 13.58 44.37x ± 14.04 14.00yb ± 5.31 managemental practices adopted by dog owners in The different dog food ingredients such as milk, Rice (g) 13.33 ± 6.31 7.81 ± 4.55 10.00 ± 4.42 10.00 ± 5.57 Barnala and Mansa districts of Punjab. Therefore, this study chapatti, pulses, fruits, meat, eggs, vegetables etc. were Fruits and vegetables was planned with the objective to determine the nutritional a b collected from the owner at his/her location in an insulated Vegetables (g) 39.33 ± 8.44 54.38 ± 10.10 19.83 ± 5.98 22.83 ± 9.13 and managemental practices adopted by dog owners. Non- vegetarian container to maintain the freshness of food and were Meat (g) 55.00 ± 18.93 112.50a ± 25.70 54.67x ± 13.82 13.33yb ± 6.31 MATERIALS AND METHODS brought to the Department of Animal Nutrition. A total of y xa x yb Bone (g) 15.83 ± 4.87 33.59 ± 7.58 20.33 ± 4.51 6.67 ± 3.16 15 samples were collected from the owners of Barnala and a x yb To fulfill the objective of the study, the present Egg (QT) 1.47 ± 0.27 1.94 ± 0.23 0.90 ± 0.19 0.27 ± 0.12 investigation was carried out to study the different feeding Mansa districts of Punjab. Value in parenthesis represents number of respondents; Figures with different superscripts in a row differ significantly (P0.05) practices adopted by dog owners in Barnala and Mansa Analysis of Dog food samples l x,y depicts the significant difference in variable between urban and rural areas of same district. district. A complete diet was prepared according to their l A,B depicts the significant difference in variable between urban areas of both district. Corresponding author: [email protected] *Corresponding author: [email protected] proportion in the diet of dog as offered by their owner. The l a,b depicts the significant difference in variable between rural areas of both district.

84 85

CONTRIBUTORS MAY NOTE CONTRIBUTORS MAY NOTE l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians THE HARYANA VETERINARIAN THE HARYANA VETERINARIAN l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. engaged in Veterinary Profession. l Please follow strictly the format of 'The Haryana Veterinarian' for manuscriptEditors/Editorial Board Members are highly thankful to Editors/Editorial Board Members are highly thankful to l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript writing/submission. writing/submission. all the distinguished referees who helped us in the all the distinguished referees who helped us in the l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. evaluation of articles. We request them to continue to evaluation of articles. We request them to continue to Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. extend their co-operation and be prompt in future to give extend their co-operation and be prompt in future to give l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick response. their valuable comments on the articles for timely their valuable comments on the articles for timely l Please mention your article reference number in all correspondence for a quick response. l We solicit your co-operation. publication of the journal. publication of the journal. l We solicit your co-operation. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Sciences, LUVAS, Hisar-125004. Editors Editors

CONTRIBUTORS MAY NOTE THE HARYANA VETERINARIAN l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians Editors/Editorial Board Members are highly thankful to THE HARYANA VETERINARIAN engaged in Veterinary Profession. CONTRIBUTORS MAY NOTE all the distinguished referees who helped us in the THE HARYANA VETERINARIAN l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript evaluation of articles. We request them to continue to Editors/Editorial Board Members are highly thankful to writing/submission. l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians Editors/Editorial Board Members are highly thankful to l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of engaged in Veterinary Profession. extend their co-operation and be prompt in future to give all the distinguished referees who helped us in the Veterinary Sciences, along with each article. l all the distinguished referees who helped us in the Please follow strictly the format of 'The Haryana Veterinarian' for manuscript their valuable comments on the articles for timely evaluation of articles. We request them to continue to l After revision, please return the revised manuscript and rebuttal at the earliest. writing/submission. evaluation of articles. We request them to continue to l l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of publication of the journal. extend their co-operation and be prompt in future to give Please mention your article reference number in all correspondence for a quick extend their co-operation and be prompt in future to give response. Veterinary Sciences, along with each article. their valuable comments on the articles for timely l We solicit your co-operation. l After revision, please return the revised manuscript and rebuttal at the earliest. their valuable comments on the articles for timely publication of the journal. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, l Please mention your article reference number in all correspondence for a quick publication of the journal. Department of Veterinary Public Health and Epidemiology, College of Veterinary response. Sciences, LUVAS, Hisar-125004. l We solicit your co-operation.

Editors l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Editors THE HARYANA VETERINARIAN CONTRIBUTORS MAY NOTE

Editors/Editorial Board Members are highly thankful to l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. THE HARYANA VETERINARIAN all the distinguished referees who helped us in the CONTRIBUTORS MAY NOTE l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript Editors/Editorial Board Members are highly thankful to evaluation of articles. We request them to continue to writing/submission. l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. extend their co-operation and be prompt in future to give l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of all the distinguished referees who helped us in the Veterinary Sciences, along with each article. evaluation of articles. We request them to continue to l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript their valuable comments on the articles for timely l After revision, please return the revised manuscript and rebuttal at the earliest. writing/submission. extend their co-operation and be prompt in future to give l publication of the journal. l Please mention your article reference number in all correspondence for a quick Please pay processing fee of Rs. 1000/- online in the account of Dean, College of response. their valuable comments on the articles for timely Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. l We solicit your co-operation. publication of the journal. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, l Please mention your article reference number in all correspondence for a quick Department of Veterinary Public Health and Epidemiology, College of Veterinary response. Sciences, LUVAS, Hisar-125004. l We solicit your co-operation.

Editors l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Editors THE HARYANA VETERINARIAN Editors/Editorial Board Members are highly thankful to all the distinguished referees who helped us in the evaluation of articles. We request them to continue to extend their co-operation and be prompt in future to give their valuable comments on the articles for timely publication of the journal.

CONTRIBUTORS MAY NOTE l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript writing/submission. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick response. l We solicit your co-operation. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Editors

15 22 Research Article

Haryana Vet. (Dec., 2019) 58(2), 166-169 Research Article

ABSTRACT Fig 2: Transmission electron photomicrograph of monocyte of dog Present study comprised of 72 crossbred cows (group I= 60 endometritic and group II=12 healthy) at 30±2days postpartum. The Fig. 1. Lateral view of ribs (13 numbers) of left side of adult female showing heterochromatin (a), euchromatin (b), cytoplasmic process (c), polymorphonuclear neutrophils (PMN) cell coun  Vacuole and nuclear membrane. Bule bull (Boselaphus tragocamelus) Uranyl acetate and lead citrate × 25500

Fig. 1. Histogram depicting frequency distribution of animal right score of respondents

Figure 1: Cyclic conditions for PCR profiling for detection of Salmonella genes

Fig. 1. Semilogarithmic plot of plasma concentration time profile of ASSOCIATION OF SEMEN TRAITS IN amoxicillin and cloxacillin following single dose (10 mg/kg) i.v. and Marinelli, L., Adamelli, S., Normando, S. and Bono, G. i.m. administration in sheep (n=4) (2007). Quality of life of the pet dog: Influence of CONSECUTIVE EJACULATES WITH FSH-β GENE owner and dog’s characteristics. Appl. Anim. Behav. POLYMORPHISM IN HOLSTEIN-FRIESIAN Sci. 108(1- 1 2 CROSSBRED BULLS FROM INDIA VIJAY KADAM, ABH

trus synchronizathod that synchronizes ovulations is Corresponding author: [email protected] named briefly as “Ovsynch” (Pursley et al., 1995). The Clinical Article study was aimed to evaluate the efficacy of different methods of estrus sync

Haryana Vet. (Dec., 2019) 58(2), 166-169 Research Article rural dogs of Barnala district shows that there was no- protein and 25% feed sample were having less than 18% Table 3 research centre under the sub-head entitled “Nutritional significant (P0.05) differences with respect to milk, milk protein. Whereas, analysis of feed sample of Mansa district Physico–chemical properties of dog diet of Barnala and and processing interventions for developing pet foods” COMPARATIVE EFFICACY OF SYNCHRONIZATION PROTOCOLS FOR IMPROVING product, sweet and curd consumption between urban and showed that 42.8% of feed samples were in range of 18- Mansa districts of Punjab under the ageis of Department of Biotechnology, Govt. of FERTILITY IN POSTPARTUM CROSSBRED DAIRY COWS rural areas of this district. Average quantity of dal offered to 22% and 57.14% of feed sample having less than 18% Parameter Districts India vide order no BT/ADV/Canine Health/GADVASU/ RATNAPARKHI A.R., DESHMUKH S.G.*, BIRADE H.S., KALE V.B., HARKAL S.B. and JADHAO A.D. dog in urban areas (44.37g) was higher (P0.05) as compared protein. On an average, it can be ascertained that almost Barnala Mansa 2017-18. Department of Animal Reproduction, Gynaecology and Obstetrics, 70% food offered to dogs were meeting the crude protein to rural areas (14.00g). Non-significant (P0.05) difference pH 3.94±0.06 4.11±0.17 REFERENCES Post Graduate Institute of Veterinary and Animal Sciences, Akola-444104, India requirement. Following fat analysis of feed sample, it was b a Corresponding author: [email protected] in feeding of chapatti, rice and vegetable was observed in FFA (<2%) 0.11 ±0.02 0.28 ±0.05 Davis, B.W., Alie, K., Fielding, W.J., Morters, M. and Galindo, F. noticed that 100% of the feed samples were having more b a Received: 20.03.2019; Accepted: 13.05.2019 rural and urban areas. Regarding non vegetarian diet, it PV (1-2) 0.39 ±0.07 0.83 ±0.11 (2007). Preliminary observations on the characteristics of the than 5% fat in both the districts. The fibre analysis showed ab owned dog population in Roseau, Dominica. J. Appl. Anim. 182 was noticed that consumption of meat, bone and egg is SPC(<7 log 10cfu/gm) 2.52±0.10 2.62 ±0.09 ABSTRACT that 62.5% of feed samples of Barnala district were having Coliform (<3 log 10cfu/gm) 2.67±0.09 2.38±0.24 Welf. Sci. 10(2): 141-51. th The objective of present research work was to study the efficacy of two different synchronization protocols for improving the fertility in greater (P0.05) in urban areas (meat 54.67g, bone 20.33g, fiber less than 3.5% and 37.5% of samples were having Livestock Census. (2012). 19 Indian Livestock Census, All India are being offered to dogs. The mean value of the postpartum crossbred cows. A totah protocol induces better estrus response and first service conception rate in postpartum cows. egg 0.90) with respect to rural areas (meat 13.33g, bone fiber more than 6%. Whereas feed sample of Mansa district Report, Government of India, Ministry of Agriculture microbiological parameters such as SPC and Coliforms Department of Animal Husbandry, Dairy and Fisheries Krishi Keywords: Conception rate, Double PG, Ovsynch protocol 6.67g, egg 0.27g) of Barnala district. showed that 28.57% of feed sample were having fibre were also well below the prescribed limits of the cooked Bhawan, New Delhi India pet food market growth trends and While scrutinizing the data, it was observed that, more than 6%, 42.85% having fibre 3.5- 6% and 28.6% forecast 2015-2020. dog foods. Since all the samples taken were fresh as interestingly 72.4% (Figure 1) of population of Barnala having fibre less than 3.5%. As far as calcium and phosphorus Michel, K.E. (2006). Unconventional diets for dogs and cats. Vet. Clin. owners offer fresh food to their dogs. North America Small Anim. Pract. 36: 1269-1281. and 47.3% of population of Mansa district of Punjab offer is concerned, 62.5% sample of Barnala district were It is concluded that homemade diet feeding is preferred Sawaimul, A.D., Ghule, S.S., Ali, S.Z., Sahare, M.G. and Patil, L.V. homemade feed to their dogs while not even a single owner having more than 0.5% calcium and 37.5% having less (2009). Preference for breed and feeding practices for dog is feeding exclusively commercial feed to their dog in than 0.5% calcium.100% sample of Mansa district were over readymade feed in both Barnala and Mansa districts. rearing in Nagpur city of Maharashtra. Vet. World. 2(3): 109- 110. The estrus synchronizathod that synchronizes these two districts. 27.4% dog owners of Barnala and having calcium less than 0.5%. 37.5% of samples were Chemical analysis showed that though 50% sample of Seneviratne, M., Subasinghe, D.W. and Watson, P.J. (2016). A survey of ovulations is named briefly as “Ovsynch” (Pursley et al., 52.7% of Mansa district offered both homemade and seen to have more than 0.3% phosphorus and 62.5% less Barnala district and 42.8% sample of Mansa district were meeting the protein requirement, a good balanced diet is pet feeding practices of dog owners visiting a veterinary practice 1995). The study was aimed to evaluate the efficacy of commercial feed to their pets. Davis et al. (2007) also than 0.3% phosphorus in Barnala district. Regarding in Colombo, Sri Lanka. Vet. Med. Sci. 2(2): 106-116. required to meet the demand of the dogs, may be a special different methods of estrus synchronisation protocols in reported that 75% of the dogs were fed home-cooked dog Mansa district, it was noticed that 42.85% feed sample Shakhar, C., Pattanaik, A,K., Kore, K.B., Sharma, K. (2010). Apprasial crossbred postpartum cows. food; 23% commercial dog food; 11.5% table scraps; 1.4% were having phosphorus more than 0.3% and 57.14% less vegetarian diet meeting all the nutrient levels. of feeding practices and blood metabolic profile of pet dogs reared raw meat and 2.7% other diets. In Barnala district, 68.3% than 0.3%. ACKNOWLEDGEMENT on homemade diets. Anim. Nutr. Feed Technol. 10(1): 61-73. MATERIAL AND METHODS Stockman, J., Fascetti, A.J., Kass, P.H., Larsen, J.A. (2013). Evaluation rural and 38.7% of urban population feed only vegetarian The dog food samples collected from different areas A total of 20 healthy crossbred cows at 60-90 days The authors are thankful to DBT, GOI for providing of recipes of home-prepared maintenance diets of dogs. J. Am. diet to their dogs. From the data, it is inferred that more of Barnala and Mansa were examined for physico- the funds. The survey was conducted as part of canine Vet. Med. Assoc. 242: 1500-1505. homemade feeding in Barnala district is due to the chemical (pH, Free Fatty Acids and Peroxide Value) and preference of vegetarian diet. Seneviratne et al. (2016) in microbiological (Standard Plate Count, SPC and coliforms his survey also observed that 42% of the dogs were being count) parameters (Table 3). The data of dog samples fed homemade diets only while only commercial food obtained from both the district revealed that, the pH value accounts for 18% and both homemade and commercial was less than 4 i.e. the samples were having acidic nature diets were fed to 40% of dogs. which may be due to the higher proportion of milk and The dog food samples obtained from Barnala and milk products which when dried lead to acidic conditions. Mansa districts were analyzed for various proximate The level of Free fatty acids and peroxide value were principles (Table 2). Fifteen feed samples collected from below the prescribed limits which shows that there was no urban and rural areas of both districts. During analysis of fat rancidity and good quality different food ingredients feed sample of Barnala district, it was found that 50% of Table 2 feed samples were in range of 18-22% for protein content, however, 25% feed samples were having more than 22% Chemical analysis of feed Parameters Percentage Mansa (%) Barnala (%) Protein <18 57.2 25 18-22 42.8 50 >22 0 25 Fat <5 0 0 >5 100 100 Fibre <3.5 28.6 62.5 3.5-6 42.8 0 >6 28.6 37.5 Calcium <0.5 100 37.5 >0.5 0 62.5 Fig. 1. Percent usage of Food items by dog owners of Barnala and Phosphorus <0.3 42.8 62.5 Corresponding author: [email protected] Mansa District >0.3 57.2 37.5

86 87

CONTRIBUTORS MAY NOTE CONTRIBUTORS MAY NOTE l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians THE HARYANA VETERINARIAN THE HARYANA VETERINARIAN l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. engaged in Veterinary Profession. l Please follow strictly the format of 'The Haryana Veterinarian' for manuscriptEditors/Editorial Board Members are highly thankful to Editors/Editorial Board Members are highly thankful to l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript writing/submission. writing/submission. all the distinguished referees who helped us in the all the distinguished referees who helped us in the l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. evaluation of articles. We request them to continue to evaluation of articles. We request them to continue to Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. extend their co-operation and be prompt in future to give extend their co-operation and be prompt in future to give l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick response. their valuable comments on the articles for timely their valuable comments on the articles for timely l Please mention your article reference number in all correspondence for a quick response. l We solicit your co-operation. publication of the journal. publication of the journal. l We solicit your co-operation. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Sciences, LUVAS, Hisar-125004. Editors Editors

CONTRIBUTORS MAY NOTE THE HARYANA VETERINARIAN l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians Editors/Editorial Board Members are highly thankful to THE HARYANA VETERINARIAN engaged in Veterinary Profession. CONTRIBUTORS MAY NOTE all the distinguished referees who helped us in the THE HARYANA VETERINARIAN l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript evaluation of articles. We request them to continue to Editors/Editorial Board Members are highly thankful to writing/submission. l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians Editors/Editorial Board Members are highly thankful to l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of engaged in Veterinary Profession. extend their co-operation and be prompt in future to give all the distinguished referees who helped us in the Veterinary Sciences, along with each article. l all the distinguished referees who helped us in the Please follow strictly the format of 'The Haryana Veterinarian' for manuscript their valuable comments on the articles for timely evaluation of articles. We request them to continue to l After revision, please return the revised manuscript and rebuttal at the earliest. writing/submission. evaluation of articles. We request them to continue to l l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of publication of the journal. extend their co-operation and be prompt in future to give Please mention your article reference number in all correspondence for a quick extend their co-operation and be prompt in future to give response. Veterinary Sciences, along with each article. their valuable comments on the articles for timely l We solicit your co-operation. l After revision, please return the revised manuscript and rebuttal at the earliest. their valuable comments on the articles for timely publication of the journal. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, l Please mention your article reference number in all correspondence for a quick publication of the journal. Department of Veterinary Public Health and Epidemiology, College of Veterinary response. Sciences, LUVAS, Hisar-125004. l We solicit your co-operation.

Editors l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Editors THE HARYANA VETERINARIAN CONTRIBUTORS MAY NOTE

Editors/Editorial Board Members are highly thankful to l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. THE HARYANA VETERINARIAN all the distinguished referees who helped us in the CONTRIBUTORS MAY NOTE l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript Editors/Editorial Board Members are highly thankful to evaluation of articles. We request them to continue to writing/submission. l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. extend their co-operation and be prompt in future to give l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of all the distinguished referees who helped us in the Veterinary Sciences, along with each article. evaluation of articles. We request them to continue to l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript their valuable comments on the articles for timely l After revision, please return the revised manuscript and rebuttal at the earliest. writing/submission. extend their co-operation and be prompt in future to give l publication of the journal. l Please mention your article reference number in all correspondence for a quick Please pay processing fee of Rs. 1000/- online in the account of Dean, College of response. their valuable comments on the articles for timely Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. l We solicit your co-operation. publication of the journal. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, l Please mention your article reference number in all correspondence for a quick Department of Veterinary Public Health and Epidemiology, College of Veterinary response. Sciences, LUVAS, Hisar-125004. l We solicit your co-operation.

Editors l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Editors THE HARYANA VETERINARIAN Editors/Editorial Board Members are highly thankful to all the distinguished referees who helped us in the evaluation of articles. We request them to continue to extend their co-operation and be prompt in future to give their valuable comments on the articles for timely publication of the journal.

CONTRIBUTORS MAY NOTE l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript writing/submission. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick response. l We solicit your co-operation. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Editors

15 22 Research Article

Haryana Vet. (Dec., 2019) 58(2), 166-169 Research Article

ABSTRACT Fig 2: Transmission electron photomicrograph of monocyte of dog Present study comprised of 72 crossbred cows (group I= 60 endometritic and group II=12 healthy) at 30±2days postpartum. The Fig. 1. Lateral view of ribs (13 numbers) of left side of adult female showing heterochromatin (a), euchromatin (b), cytoplasmic process (c), polymorphonuclear neutrophils (PMN) cell coun  Vacuole and nuclear membrane. Bule bull (Boselaphus tragocamelus) Uranyl acetate and lead citrate × 25500

Fig. 1. Histogram depicting frequency distribution of animal right score of respondents

Figure 1: Cyclic conditions for PCR profiling for detection of Salmonella genes

Fig. 1. Semilogarithmic plot of plasma concentration time profile of ASSOCIATION OF SEMEN TRAITS IN amoxicillin and cloxacillin following single dose (10 mg/kg) i.v. and Marinelli, L., Adamelli, S., Normando, S. and Bono, G. i.m. administration in sheep (n=4) (2007). Quality of life of the pet dog: Influence of CONSECUTIVE EJACULATES WITH FSH-β GENE owner and dog’s characteristics. Appl. Anim. Behav. POLYMORPHISM IN HOLSTEIN-FRIESIAN Sci. 108(1- 1 2 CROSSBRED BULLS FROM INDIA VIJAY KADAM, ABH

trus synchronizathod that synchronizes ovulations is Corresponding author: [email protected] named briefly as “Ovsynch” (Pursley et al., 1995). The Clinical Article study was aimed to evaluate the efficacy of different methods of estrus sync

Haryana Vet. (Dec., 2019) 58(2), 166-169 Research Article rural dogs of Barnala district shows that there was no- protein and 25% feed sample were having less than 18% Table 3 research centre under the sub-head entitled “Nutritional significant (P0.05) differences with respect to milk, milk protein. Whereas, analysis of feed sample of Mansa district Physico–chemical properties of dog diet of Barnala and and processing interventions for developing pet foods” COMPARATIVE EFFICACY OF SYNCHRONIZATION PROTOCOLS FOR IMPROVING product, sweet and curd consumption between urban and showed that 42.8% of feed samples were in range of 18- Mansa districts of Punjab under the ageis of Department of Biotechnology, Govt. of FERTILITY IN POSTPARTUM CROSSBRED DAIRY COWS rural areas of this district. Average quantity of dal offered to 22% and 57.14% of feed sample having less than 18% Parameter Districts India vide order no BT/ADV/Canine Health/GADVASU/ RATNAPARKHI A.R., DESHMUKH S.G.*, BIRADE H.S., KALE V.B., HARKAL S.B. and JADHAO A.D. dog in urban areas (44.37g) was higher (P0.05) as compared protein. On an average, it can be ascertained that almost Barnala Mansa 2017-18. Department of Animal Reproduction, Gynaecology and Obstetrics, 70% food offered to dogs were meeting the crude protein to rural areas (14.00g). Non-significant (P0.05) difference pH 3.94±0.06 4.11±0.17 REFERENCES Post Graduate Institute of Veterinary and Animal Sciences, Akola-444104, India requirement. Following fat analysis of feed sample, it was b a Corresponding author: [email protected] in feeding of chapatti, rice and vegetable was observed in FFA (<2%) 0.11 ±0.02 0.28 ±0.05 Davis, B.W., Alie, K., Fielding, W.J., Morters, M. and Galindo, F. noticed that 100% of the feed samples were having more b a Received: 20.03.2019; Accepted: 13.05.2019 rural and urban areas. Regarding non vegetarian diet, it PV (1-2) 0.39 ±0.07 0.83 ±0.11 (2007). Preliminary observations on the characteristics of the than 5% fat in both the districts. The fibre analysis showed ab owned dog population in Roseau, Dominica. J. Appl. Anim. 182 was noticed that consumption of meat, bone and egg is SPC(<7 log 10cfu/gm) 2.52±0.10 2.62 ±0.09 ABSTRACT that 62.5% of feed samples of Barnala district were having Coliform (<3 log 10cfu/gm) 2.67±0.09 2.38±0.24 Welf. Sci. 10(2): 141-51. th The objective of present research work was to study the efficacy of two different synchronization protocols for improving the fertility in greater (P0.05) in urban areas (meat 54.67g, bone 20.33g, fiber less than 3.5% and 37.5% of samples were having Livestock Census. (2012). 19 Indian Livestock Census, All India are being offered to dogs. The mean value of the postpartum crossbred cows. A totah protocol induces better estrus response and first service conception rate in postpartum cows. egg 0.90) with respect to rural areas (meat 13.33g, bone fiber more than 6%. Whereas feed sample of Mansa district Report, Government of India, Ministry of Agriculture microbiological parameters such as SPC and Coliforms Department of Animal Husbandry, Dairy and Fisheries Krishi Keywords: Conception rate, Double PG, Ovsynch protocol 6.67g, egg 0.27g) of Barnala district. showed that 28.57% of feed sample were having fibre were also well below the prescribed limits of the cooked Bhawan, New Delhi India pet food market growth trends and While scrutinizing the data, it was observed that, more than 6%, 42.85% having fibre 3.5- 6% and 28.6% forecast 2015-2020. dog foods. Since all the samples taken were fresh as interestingly 72.4% (Figure 1) of population of Barnala having fibre less than 3.5%. As far as calcium and phosphorus Michel, K.E. (2006). Unconventional diets for dogs and cats. Vet. Clin. owners offer fresh food to their dogs. North America Small Anim. Pract. 36: 1269-1281. and 47.3% of population of Mansa district of Punjab offer is concerned, 62.5% sample of Barnala district were It is concluded that homemade diet feeding is preferred Sawaimul, A.D., Ghule, S.S., Ali, S.Z., Sahare, M.G. and Patil, L.V. homemade feed to their dogs while not even a single owner having more than 0.5% calcium and 37.5% having less (2009). Preference for breed and feeding practices for dog is feeding exclusively commercial feed to their dog in than 0.5% calcium.100% sample of Mansa district were over readymade feed in both Barnala and Mansa districts. rearing in Nagpur city of Maharashtra. Vet. World. 2(3): 109- 110. The estrus synchronizathod that synchronizes these two districts. 27.4% dog owners of Barnala and having calcium less than 0.5%. 37.5% of samples were Chemical analysis showed that though 50% sample of Seneviratne, M., Subasinghe, D.W. and Watson, P.J. (2016). A survey of ovulations is named briefly as “Ovsynch” (Pursley et al., 52.7% of Mansa district offered both homemade and seen to have more than 0.3% phosphorus and 62.5% less Barnala district and 42.8% sample of Mansa district were meeting the protein requirement, a good balanced diet is pet feeding practices of dog owners visiting a veterinary practice 1995). The study was aimed to evaluate the efficacy of commercial feed to their pets. Davis et al. (2007) also than 0.3% phosphorus in Barnala district. Regarding in Colombo, Sri Lanka. Vet. Med. Sci. 2(2): 106-116. required to meet the demand of the dogs, may be a special different methods of estrus synchronisation protocols in reported that 75% of the dogs were fed home-cooked dog Mansa district, it was noticed that 42.85% feed sample Shakhar, C., Pattanaik, A,K., Kore, K.B., Sharma, K. (2010). Apprasial crossbred postpartum cows. food; 23% commercial dog food; 11.5% table scraps; 1.4% were having phosphorus more than 0.3% and 57.14% less vegetarian diet meeting all the nutrient levels. of feeding practices and blood metabolic profile of pet dogs reared raw meat and 2.7% other diets. In Barnala district, 68.3% than 0.3%. ACKNOWLEDGEMENT on homemade diets. Anim. Nutr. Feed Technol. 10(1): 61-73. MATERIAL AND METHODS Stockman, J., Fascetti, A.J., Kass, P.H., Larsen, J.A. (2013). Evaluation rural and 38.7% of urban population feed only vegetarian The dog food samples collected from different areas A total of 20 healthy crossbred cows at 60-90 days The authors are thankful to DBT, GOI for providing of recipes of home-prepared maintenance diets of dogs. J. Am. diet to their dogs. From the data, it is inferred that more of Barnala and Mansa were examined for physico- the funds. The survey was conducted as part of canine Vet. Med. Assoc. 242: 1500-1505. homemade feeding in Barnala district is due to the chemical (pH, Free Fatty Acids and Peroxide Value) and preference of vegetarian diet. Seneviratne et al. (2016) in microbiological (Standard Plate Count, SPC and coliforms his survey also observed that 42% of the dogs were being count) parameters (Table 3). The data of dog samples fed homemade diets only while only commercial food obtained from both the district revealed that, the pH value accounts for 18% and both homemade and commercial was less than 4 i.e. the samples were having acidic nature diets were fed to 40% of dogs. which may be due to the higher proportion of milk and The dog food samples obtained from Barnala and milk products which when dried lead to acidic conditions. Mansa districts were analyzed for various proximate The level of Free fatty acids and peroxide value were principles (Table 2). Fifteen feed samples collected from below the prescribed limits which shows that there was no urban and rural areas of both districts. During analysis of fat rancidity and good quality different food ingredients feed sample of Barnala district, it was found that 50% of Table 2 feed samples were in range of 18-22% for protein content, however, 25% feed samples were having more than 22% Chemical analysis of feed Parameters Percentage Mansa (%) Barnala (%) Protein <18 57.2 25 18-22 42.8 50 >22 0 25 Fat <5 0 0 >5 100 100 Fibre <3.5 28.6 62.5 3.5-6 42.8 0 >6 28.6 37.5 Calcium <0.5 100 37.5 >0.5 0 62.5 Fig. 1. Percent usage of Food items by dog owners of Barnala and Phosphorus <0.3 42.8 62.5 Corresponding author: [email protected] Mansa District >0.3 57.2 37.5

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CONTRIBUTORS MAY NOTE CONTRIBUTORS MAY NOTE l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians THE HARYANA VETERINARIAN THE HARYANA VETERINARIAN l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. engaged in Veterinary Profession. l Please follow strictly the format of 'The Haryana Veterinarian' for manuscriptEditors/Editorial Board Members are highly thankful to Editors/Editorial Board Members are highly thankful to l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript writing/submission. writing/submission. all the distinguished referees who helped us in the all the distinguished referees who helped us in the l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. evaluation of articles. We request them to continue to evaluation of articles. We request them to continue to Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. extend their co-operation and be prompt in future to give extend their co-operation and be prompt in future to give l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick response. their valuable comments on the articles for timely their valuable comments on the articles for timely l Please mention your article reference number in all correspondence for a quick response. l We solicit your co-operation. publication of the journal. publication of the journal. l We solicit your co-operation. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Sciences, LUVAS, Hisar-125004. Editors Editors

CONTRIBUTORS MAY NOTE THE HARYANA VETERINARIAN l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians Editors/Editorial Board Members are highly thankful to THE HARYANA VETERINARIAN engaged in Veterinary Profession. CONTRIBUTORS MAY NOTE all the distinguished referees who helped us in the THE HARYANA VETERINARIAN l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript evaluation of articles. We request them to continue to Editors/Editorial Board Members are highly thankful to writing/submission. l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians Editors/Editorial Board Members are highly thankful to l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of engaged in Veterinary Profession. extend their co-operation and be prompt in future to give all the distinguished referees who helped us in the Veterinary Sciences, along with each article. l all the distinguished referees who helped us in the Please follow strictly the format of 'The Haryana Veterinarian' for manuscript their valuable comments on the articles for timely evaluation of articles. We request them to continue to l After revision, please return the revised manuscript and rebuttal at the earliest. writing/submission. evaluation of articles. We request them to continue to l l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of publication of the journal. extend their co-operation and be prompt in future to give Please mention your article reference number in all correspondence for a quick extend their co-operation and be prompt in future to give response. Veterinary Sciences, along with each article. their valuable comments on the articles for timely l We solicit your co-operation. l After revision, please return the revised manuscript and rebuttal at the earliest. their valuable comments on the articles for timely publication of the journal. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, l Please mention your article reference number in all correspondence for a quick publication of the journal. Department of Veterinary Public Health and Epidemiology, College of Veterinary response. Sciences, LUVAS, Hisar-125004. l We solicit your co-operation.

Editors l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Editors THE HARYANA VETERINARIAN CONTRIBUTORS MAY NOTE

Editors/Editorial Board Members are highly thankful to l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. THE HARYANA VETERINARIAN all the distinguished referees who helped us in the CONTRIBUTORS MAY NOTE l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript Editors/Editorial Board Members are highly thankful to evaluation of articles. We request them to continue to writing/submission. l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. extend their co-operation and be prompt in future to give l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of all the distinguished referees who helped us in the Veterinary Sciences, along with each article. evaluation of articles. We request them to continue to l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript their valuable comments on the articles for timely l After revision, please return the revised manuscript and rebuttal at the earliest. writing/submission. extend their co-operation and be prompt in future to give l publication of the journal. l Please mention your article reference number in all correspondence for a quick Please pay processing fee of Rs. 1000/- online in the account of Dean, College of response. their valuable comments on the articles for timely Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. l We solicit your co-operation. publication of the journal. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, l Please mention your article reference number in all correspondence for a quick Department of Veterinary Public Health and Epidemiology, College of Veterinary response. Sciences, LUVAS, Hisar-125004. l We solicit your co-operation.

Editors l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Editors THE HARYANA VETERINARIAN Editors/Editorial Board Members are highly thankful to all the distinguished referees who helped us in the evaluation of articles. We request them to continue to extend their co-operation and be prompt in future to give their valuable comments on the articles for timely publication of the journal.

CONTRIBUTORS MAY NOTE l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript writing/submission. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick response. l We solicit your co-operation. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Editors

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