DOCSLIB.ORG

A Fourier Transform Infrared Spectroscopic Investigation of Macrovipera Lebetina Lebetina and M

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
A Fourier Transform Infrared Spectroscopic Investigation of Macrovipera Lebetina Lebetina and M RESEARCH ARTICLE Eur J Biol 2020; 79(1): 14-22 A Fourier Transform Infrared Spectroscopic Investigation of Macrovipera lebetina lebetina and M. l. obtusa Crude Venoms* Nasit Igci1,2 , Fatma Duygu Ozel Demiralp3,4 1Nevşehir Hacı Bektaş Veli University, Faculty of Science and Arts, Department of Molecular Biology and Genetics, Nevşehir, Turkey 2Nevşehir Hacı Bektaş Veli University, Science and Technology Application and Research Center, Nevşehir, Turkey 3Ankara University, Biotechnology Institute, Ankara, Turkey 4Ankara University, Faculty of Engineering, Department of Biomedical Engineering, Ankara, Turkey ORCID IDs of the authors: N.I. 0000-0001-6151-808X; F.D.O.D. 0000-0002-1798-7951 Please cite this article as: Igci N, Ozel Demiralp FD. A Fourier Transform Infrared Spectroscopic Investigation of Macrovipera lebetina lebetina and M. l. obtusa Crude Venoms. Eur J Biol 2020; 79(1): 14-22. DOI: 10.26650/EurJBiol.2020.0039 ABSTRACT Objective: Snake venoms are rich sources of bioactive molecules and have been investigated using various bioanalytical methods. Fourier transform infrared (FTIR) spectroscopy is a sensitive method that can be used to analyze biological samples. The aim of this study is to apply the FTIR spectroscopy method for the characterization of snake venom. Materials and Methods: The study characterized the lyophilized crude venoms of Macrovipera lebetina lebetina and M. l. obtusa by FTIR spectroscopy coupled with attenuated total reflectance (ATR) method in the mid-infrared region and compared the spectra between the two subspecies. The band area and intensity values were calculated for comparison and wavenumbers were detected by automated peak picking. Additionally, the study analyzed the secondary structure of venom proteins by using the second derivative spectra. Results: The study detected fourteen major and minor peaks in absorbance spectra which were assigned to various biomolecules such as proteins, carbohydrates, and nucleic acids. Four major sub-bands were observed in the second derivative spectra of Amide I-II region indicating different protein secondary structures. It was observed that there are some quantitative differences and peak shifts between the spectra of venoms of two subspecies, indicating the alteration of biomolecules. Conclusion: To the best of our knowledge, this is the first report of the use of the FTIR-ATR spectroscopy method focusing solely on the characterization of crude snake venoms in literature, accompanied with detailed peak assignment and protein secondary structure analysis. As a preliminary reference study, the results showed the usefulness of FTIR-ATR spectroscopy for the physicochemical characterization of lyophilized snake venom. Keywords: Fourier transform infrared spectroscopy, snake venom, viper, Macrovipera lebetina INTRODUCTION protease inhibitor peptides) (1-3). Snake venoms also contain some minor compounds such as carbohydrates, Snake venoms are complex molecular cocktails consist- nucleosides, biogenic amines, various carboxylic acids ing mainly of bioactive peptides and proteins including and inorganic elements (4,5). Snake venoms, an import- enzymes (such as serine and metalloproteinase, phos- ant source of bioactive proteins, have attracted research- pholipase A2, hyaluronidase, L-amino acid oxidase, nu- ers in the past. Drugs and diagnostic kits were developed cleotidase), non-enzymatic proteins (e.g. cysteine-rich using snake venom peptides and proteins (2,6-8). secretory protein, disintegrin, vascular endothelial growth factor, nerve growth factor, C-type lectin) and Macrovipera lebetina (Serpentes: Viperidae: Viperinae) is smaller peptides (e.g. bradykinin-potentiating peptides, a relatively big viper species which could reach up to * This paper was produced from the M.Sc. thesis of the first author. Part of this paper was presented at 16th National Biotechnology Congress (13-16 December 2009, Antalya, Turkey). Corresponding Author: Nasit Igci E-mail: [email protected] Submitted: 06.11.2019 • Revision Requested: 13.12.2019 • Last Revision Received: 10.03.2020 • Accepted: 11.03.2020 © Copyright 2020 by The Istanbul University Faculty of Science • Available online at http://ejb.istanbul.edu.tr • DOI: 10.26650/EurJBiol.2020.0039 14 15 Eur J Biol 2020; 79(1): 14-22 Igci and Ozel Demiralp. A FTIR Spectroscopic Investigation of Macrovipera lebetina Crude Venom 2 meters long and has a wide geographical range from north- published study focusing solely on the application of FTIR spec- ern Africa to Pakistan and Kashmir, reaching to Kazakhstan and troscopy for the characterization of crude snake venoms which Dagestan (southwestern Russia) in the North, including Turkey provides detailed band assignments, to the best of authors’ and Cyprus (9-11). Although the taxonomic status of some sub- knowledge. The present study aimed to make physicochemical species is still in question, it is accepted that nominate taxon M. characterization of crude snake venoms by FTIR-ATR spectros- l. lebetina (Linnaeus, 1758) inhabits Cyprus (11-13). The south- copy using the venoms of M. l. lebetina and M. l. obtusa as rep- ern and southeastern Anatolia populations were treated as M. l. resentative materials and assess the method’s usefulness in the euphratica (Martin, 1838) in the past but this name is generally field of toxinology. synonymized under M. l. obtusa (Dwigubsky, 1832) today (12). M. l. obtusa has a geographical range from Mersin to south- MATERIALS AND METHODS eastern, southern, and northeastern Anatolia in Turkey while Venom Samples M. l. lebetina is restricted to Cyprus island (10-13). M. lebetina is In this study, we used the pooled venoms of two adult M. l. lebetina largely responsible for the harmful snakebite-cases in its world- and two adult M. l. obtusa individuals collected from The Turk- wide range as well as in Turkey and it is the only venomous vi- ish Republic of Northern Cyprus (Selvilitepe and Dikmen) and per species occurring in Cyprus (12,14,15). southeast Turkey (Egil/Diyarbakir and Suruc/Sanliurfa), respec- Fourier transform infrared (FTIR) spectroscopy is a fast, robust, tively. The venom samples were extracted from vipers using a sensitive, and widely-used vibrational spectroscopy method. The paraffin-covered laboratory beaker and without applying any working principle is the formation of different vibrational modes pressure on the venom glands. Authors had ethical permission of the chemical groups in molecules after absorbing infrared ra- for venom extraction at the time of the study (Ege University diation (16,17). Due to the non-destructive and non-invasive na- Animal Experiments Ethics Committee, permit number 2010- ture of infrared radiation, this method is widely applied to biolog- 43). After extraction, venom samples were centrifuged at 2000 ical and biomedical studies today (18-21). Each vibration type has × g for 10 min at 4°C. Supernatants were collected, frozen at its specific frequency of absorption which can be associated with -80°C and then lyophilized by using a bench-top freeze-dryer various bioorganic molecules in biological samples. The amounts (Millrock Technology, Kingston, NY, USA). Lyophilized samples of biomolecules can be assessed in a comparative manner using were kept at 4°C until used. the band intensities and areas (18,19). Moreover, information re- FTIR Data Collection and Evaluation garding the secondary structures of proteins can be obtained by Lyophilized venom samples were measured using a universal spectral interpretation (17,19,20,22). attenuated total reflectance (ATR) cell (Pike Technologies, Wis- Since the major elements of crude snake venoms are proteins consin, USA) connected to an FTIR device (Bruker Tensor 27, and peptides, researchers have been focusing on their pro- Bruker Optics GmbH, Ettlingen, Germany) equipped with a teomic characterization using various bioanalytical methods liquid nitrogen cooled photovoltaic mercury cadmium tellu- suitable for protein separation and analysis such as polyacryl- ride detector. High-purity nitrogen gas was purging during all amide gel electrophoresis, liquid chromatography, and mass the measurements. Air was subtracted automatically before all spectrometry (3,23-28). Venom of the subspecies of M. lebetina sample measurements. Spectra of the venom samples were re- has also been an important source in many studies aimed to corded in the mid-infrared region, between 4500-850 cm-1 wav- purify proteins and make proteomic and biological character- enumbers and interferograms were accumulated for 50 scans ization (3,25,29). Major protein families of M. lebetina venom at 4 cm-1 resolutions at room temperature in the single-bounce ATR mode. A small amount of the sample, enough to cover the are metalloproteinase, serine proteinase, phospholipase A2, L-amino acid oxidase, hyaluronidase, disintegrin, C-type lectin, ZnSe ATR crystal, were placed and compressed with a clamp. cysteine-rich secretory protein, vascular endothelial growth Each sample was measured in triplicate and the average spectra factor, nerve growth factor, bradykinin-potentiating peptides, was used for analyses. and protease inhibitor peptides (3,25,29). These proteins give the venom its unique pharmacological properties such as anti- Spectral data interpretation was performed by OPUS 5.5 soft- coagulant, cytotoxic and antimicrobial activities. ware (Bruker). Automated peak picking was performed in order to determine wavenumber values. Min-max normalization was The Fourier Transform Infrared Spectroscopy
Load more

Recommended publications
  • Solenodon Genome Reveals Convergent Evolution of Venom in Eulipotyphlan Mammals
    Solenodon genome reveals convergent evolution of venom in eulipotyphlan mammals Nicholas R. Casewella,1, Daniel Petrasb,c, Daren C. Cardd,e,f, Vivek Suranseg, Alexis M. Mychajliwh,i,j, David Richardsk,l, Ivan Koludarovm, Laura-Oana Albulescua, Julien Slagboomn, Benjamin-Florian Hempelb, Neville M. Ngumk, Rosalind J. Kennerleyo, Jorge L. Broccap, Gareth Whiteleya, Robert A. Harrisona, Fiona M. S. Boltona, Jordan Debonoq, Freek J. Vonkr, Jessica Alföldis, Jeremy Johnsons, Elinor K. Karlssons,t, Kerstin Lindblad-Tohs,u, Ian R. Mellork, Roderich D. Süssmuthb, Bryan G. Fryq, Sanjaya Kuruppuv,w, Wayne C. Hodgsonv, Jeroen Kooln, Todd A. Castoed, Ian Barnesx, Kartik Sunagarg, Eivind A. B. Undheimy,z,aa, and Samuel T. Turveybb aCentre for Snakebite Research & Interventions, Liverpool School of Tropical Medicine, Pembroke Place, L3 5QA Liverpool, United Kingdom; bInstitut für Chemie, Technische Universität Berlin, 10623 Berlin, Germany; cCollaborative Mass Spectrometry Innovation Center, University of California, San Diego, La Jolla, CA 92093; dDepartment of Biology, University of Texas at Arlington, Arlington, TX 76010; eDepartment of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138; fMuseum of Comparative Zoology, Harvard University, Cambridge, MA 02138; gEvolutionary Venomics Lab, Centre for Ecological Sciences, Indian Institute of Science, 560012 Bangalore, India; hDepartment of Biology, Stanford University, Stanford, CA 94305; iDepartment of Rancho La Brea, Natural History Museum of Los Angeles County, Los Angeles,
    [Show full text]
  • Prof. AYŞE NALBANTSOY Opfefricseo Pnhaol Nien:F +Or9m0 2A3t2io 3N11 5807 Efmaxa Ipl:H Oaynsee
    Prof. AYŞE NALBANTSOY OPfefricseo Pnhaol nIen:f +or9m0 2a3t2io 3n11 5807 EFmaxa iPl:h oaynsee:. n+al9b0a n2t3s2o y3@11e g5e8.e1d1u .tr AWdedbr:e hstst:p sE:g/e/ aÜvneisviesr.esgitee.esid, uM.türh/aeynsdeis.nliakl bFaanktüsloteysi, Biyomühendislik Bölümü 35100, Bornova-İzmir EDodcutocraatteio, Eng eI nÜfnoivremrsiatetsioi, nTurkey 2001 - 2008 UPonsdtegrrgardaudautaet, eE, gEeg Üe nÜinveivresritseitsei,s Ti, uTrukrekye 1y 919979 3- 2- 0109097 FEnogrliesihg, nB2 L Uapnpgeru Iangteersmediate Dissertations aDnotciktoorra üter,e Stiamlmi, oEngel lÜan eivneterrsiitiedsiis, ,2 S0a0lm8onella gallinarum, Salmonella pullorum O ve H antijenlerine karşı monoklonal HPoesmtgargalüdtuinaates,y İoznm İirn hvieb Çiseyvorne s(iHnId)eTne sTtelemri nil eE dMiluekna Kyeasnealtil ıK Soenrturmollüa,r Eıngdea Ü Nneivwecrasisttelesi ,H 2a0s0ta0lığı Antikorlarının ELISA ve RLiefes Secaierncche sA, Brieoateschnology, Microbiology, Immunology, Natural Sciences Academic Titles / Tasks APrsosofecsiastoer ,P Ergoefe Ussnoivre, rEsgiety Ü, Mniüvheresnidteislii, kM Füahkeünltdeissil,i kB iFyaokmüülthesein, d2i0s1li2k B- Cöolünmtiün,u 2e0s20 - Continues Research Assistant, PEhgDe ,Ü Engiev eÜrnsiivteesris, iMteüshi, eMnüdhiselinkd Fisalkikü Fltaeksiü, lBteiysoi, mBiüyhoemnüdhiselinkd Bisölilkü,m 2ü0,0 280 -0 C3o -n 2ti0n0u8es Courses İHmamyvüanno Dteorkaup öKtüikltleürilne rGi,e Ulinştdireirlmgreasdi uvaet eÜ, r2e0ti1m8i ,- P 2o0s1tg9raduate, 2018 - 2019 İBnİ TVİiRtrMoE H TüEcZreİ, KUünldtüerügr Madoudaetlele, r2i0, P1o8s -t g2r0a1d9uate, 2018 - 2019 BFiİzYyOoBloEjNi,
    [Show full text]
  • Venomics: a Mini-Review
    Review Venomics: A Mini-Review David Wilson * and Norelle L. Daly ID Centre for Biodiscovery and Molecular, Development of Therapeutics, AITHM, James Cook University, Cairns, QLD 4878, Australia; [email protected] * Correspondence: [email protected]; Tel.: +61-7-4232-1707 Received: 1 June 2018; Accepted: 18 July 2018; Published: 23 July 2018 Abstract: Venomics is the integration of proteomic, genomic and transcriptomic approaches to study venoms. Advances in these approaches have enabled increasingly more comprehensive analyses of venoms to be carried out, overcoming to some extent the limitations imposed by the complexity of the venoms and the small quantities that are often available. Advances in bioinformatics and high-throughput functional assay screening approaches have also had a significant impact on venomics. A combination of all these techniques is critical for enhancing our knowledge on the complexity of venoms and their potential therapeutic and agricultural applications. Here we highlight recent advances in these fields and their impact on venom analyses. Keywords: venomics; genomics; transcriptomics; proteomics; pharmacomics; bioinformatics 1. Introduction The complex mixtures of diverse, selective and potent natural products found in the venom of venomous creatures has garnered significant interest and is well-published as a source of potential therapeutic leads [1–4]. This interest stems from >50% of all approved drugs arising from natural products or their derivatives [5], including six venom-derived drugs approved by the Food and Drug Administration (FDA) [6]. In addition to therapeutic potential, compounds from venoms also have potential as bioinsecticides [7]. Although the natural chemical libraries contained within venoms are often touted as rich sources of therapeutic and bioinsecticide leads, one of the major challenges facing venom researchers has been the characterization of molecules in highly complex venom mixtures [8].
    [Show full text]
  • T.C. Nevşehir Haci Bektaş Veli Üniversitesi Fen Bilimleri Enstitüsü
    T.C. NEVŞEHİR HACI BEKTAŞ VELİ ÜNİVERSİTESİ FEN BİLİMLERİ ENSTİTÜSÜ MACROVIPERA LEBETINA OBTUSA (KOCA ENGEREK) BİREYSEL ZEHİR VARYASYONUNUN PROTEİN PROFİLİ VE FOSFOLİPAZ A2 ENZİM AKTİVİTESİ AÇISINDAN ARAŞTIRILMASI Tezi Hazırlayan: Şeyma EROĞLU Tez Danışmanı: Dr. Öğretim Üyesi Naşit İĞCİ Biyoloji Anabilim Dalı Yüksek Lisans Tezi Mayıs 2019 NEVŞEHİR T.C. NEVŞEHİR HACI BEKTAŞ VELİ ÜNİVERSİTESİ FEN BİLİMLERİ ENSTİTÜSÜ MACROVIPERA LEBETINA OBTUSA (KOCA ENGEREK) BİREYSEL ZEHİR VARYASYONUNUN PROTEİN PROFİLİ VE FOSFOLİPAZ A2 ENZİM AKTİVİTESİ AÇISINDAN ARAŞTIRILMASI Tezi Hazırlayan: Şeyma EROĞLU Tez Danışmanı: Dr. Öğretim Üyesi Naşit İĞCİ Biyoloji Anabilim Dalı Yüksek Lisans Tezi Mayıs 2019 NEVŞEHİR TEŞEKKÜR Bu çalışmanın gerçekleştirilmesinde, yüksek lisans öğrenimim boyunca değerli bilgilerini benimle paylaşan, yardımlarını esirgemeyen, her daim yol gösteren saygı değer Danışman Hocam Naşit İĞCİ’ye; Kullanılan zehir materyallerinin elde edilmesindeki desteği için Doç. Dr. Mehmet Zülfü YILDIZ’a (Adıyaman Üniversitesi Fen-Edebiyat Fakültesi Biyoloji Bölümü); Laboratuvar imkanları ve analiz hizmetlerinden faydalandığımız Nevşehir Hacı Bektaş Veli Üniversitesi Bilim ve Teknoloji Uygulama ve Araştırma Merkezi ve Ankara Üniversitesi Biyoteknoloji Enstitüsü’ne; “Temel Protein Analizleri Laboratuvar Alt Yapısının Kurulması” başlıklı proje ile (Proje No: NEÜADP 16F2) tez çalışmalarında kullanılan bazı ekipmanların temin edilmesindeki desteği için Nevşehir Hacı Bektaş Veli Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü’ne; Her zaman yanımda olan ve desteğini esirgemeyen sevgili anneme sonsuz teşekkür ederim. iii MACROVIPERA LEBETINA OBTUSA (KOCA ENGEREK) BİREYSEL ZEHİR VARYASYONUNUN PROTEİN PROFİLİ ve FOSFOLİPAZ A2 ENZİM AKTİVİTESİ AÇISINDAN ARAŞTIRILMASI (Yüksek Lisans Tezi) Şeyma EROĞLU NEVŞEHİR HACI BEKTAŞ VELİ ÜNİVERSİTESİ FEN BİLİMLERİ ENSTİTÜSÜ Mayıs 2019 ÖZET Yılan zehirleri, içeriğinde çok farklı özelliklerde biyoaktif protein ve peptitleri içermesi bakımından günümüzde üzerinde çok sayıda araştırma yapılan önemli bir doğal üründür.
    [Show full text]
  • Prof. BAYRAM GÖÇMEN
    Prof. BAYRAM GÖÇMEN Personal Information EFmaxa iPl:h obanyer:a +m9.g0o c2m32e n3@99e g1e0.e3d6u .tr AWdedbr:e hstst:p :E/g/ew Üwnwiv.bearsyirtaemsi gFoecnm Feank.cüoltmes/i Biyoloji Bölümü Zooloji Anabilim Dalı 35100 Bornova, İzmir Biography sBcohrono iln ( BÇaonstaaknkcail, eG (üKzaenlytuorut)/-TLRimNaCs)s ionl 1/ 9C7y7p,r musid, 2d3ler dan Dde scecmobnedra r1y9 6sc5h. oGorlasd (uGaütezdel yfruormt K Buorstutalunsc im Fidkrdil eK ascrhayoeoll parnidmary EGnügzienlyeueritn Kgu arntudl uAsr csheictoenctduarrey, Ascrhchoiotel, cTtuRrNeC D) einp a1r9tm80e natn fdo 1r 9tw83o, sremspeesctteirvse.l yIn. I2n1 1s9t 8A3u,g juosint e1d9 8G5a zcio Umnpivletresdit yh iFsa ocbulitgya otofry Bmiioliltoagry Dseerpvaicretm ine nTtR aNt Cth. Ien h 2e0atdh oJuf nheis 1c9la8s9s g(aralsdou amteodn fgr othme tshteu dSencttiso onf o tfh Ze oFoalcouglyty, Aaengde tahne U Dneivpearrstmitye nFta)c uwlittyh o 9f1 S/c1ie0n0ce Spcoii. nBtiso. lIong 0y6 Dthe pFaerbtmrueanrty, u1p9o9n1 bhei nbge csaumccee asfnu “l Rine stheaer ecxha Ams sgiisvtean ti”n i nO ctthoeb Zeor o1l9o9g0y . SIenc 1ti6otnh. oDfe Acemgebaenr U1n9i9v1e rhseit yc oFmacp. loetfed fhrios mM Sthce t Rhuemsiesn [ eonf tCitalettdle “”T] hine AMeogrepahno Ulongivicearls aitnyd I nCsyttiotuloteg iocaf lN Sattruurcatul arensd oAfp Spolmiede Sycimenbcioetsi,c B Cioililaotgeys S(eIscotiotrni,c Zhoao slopgpy.) (ETdRuNcaCt)io /n Caly Pprougs rcaitmiz.e Ins 1h9ip9s6. he became a Turkish citizen, in addition to his Turkish Republic of Northern Cyprus RInu 1m6etnh CSielipatteems boef rE 1p9id9i6n hiuem co Cmrpawlelteyd, h1i9s2 P3h aDn dth Oepshisr y[eonstcitoleledx “ MStoerinp,h 1o8lo5g8i c(aCl ialinodp Thaoxroan:o Emnitcoald Iinnvieosmtigoartpiohnisd ao)n” ]th ine “ADeogcetaonr Uonf iPvheirlsoistyo pInhsyt”i.t uInte 0 o1fs Nt Dateucreaml abnedr 1A9p9p7li ehde Swcaiesn acpeps,o Biniotelodg tyo S tehcet i“oTne, aZcohoelrosg Syt aEfdf”u caast aionn “aAl sPsriostgarnatm P raonfde sbseocra”m aet a Pthreo Zfeososloogry” .
    [Show full text]
  • Prof.Dr. BAYRAM GÖÇMEN
    Prof.Dr. BAYRAM GÖÇMEN Kişisel Bilgiler EF-apxo Tsetale: fboanyura: m+.9go0c 2m3e2n 3@9e9g 1e.0e3d6u .tr PWoesbta: hAtdtpr:e//swi: wEwge.b Üanyirvaemrsgiotecsmi eFne.nc oFmak/ültesi Biyoloji Bölümü Zooloji Anabilim Dalı 35100 Bornova, İzmir Biyografi 2(B3o.1st2a.n1c9ı,6 G5ü'zdeel yÇuarnta/k Kk.aKl.eT .(CK.)a'nduu, 1)-9L8im0a'dseo lG (üLzeeylymuorst uKnu)r/tKuılburşı sO'rdtaa odkouğlduu'n. 1u 9(7şi7m dyıilkıni addaı yBloas; tŞaenhciıt FTiukrgi uKta Orarytaeol kİluklouk)u lu Fvea k1ü9lt8e3si' dMei mGüarzleıkly Burötl üKmurütnudlueş 1 L yisıle ösiğ'nrei n(Kim.K g.Tö.Crd) üb.i t2ir1d.0i. 81.918938'5dtea Kriahyinıtdlaen Adsığkıe Grlaikzi hÜiznmivetrisniit eKs.Ki M.Tü.Ch.'edned islik Mimamrlık otarmtaalammlaad iıl.e 2 0B.ö0l6ü.m19 v8e9 'Fdaak Eü. lÜte. F Beinr iFnackiüslit eoslai rBaiyko mloejzi uBnö loülmduü.' n0ü6n Ş Zuoboalot j1i A9n9a1b'diliem, EDkaimlı' n1d9a9n0 1'd0a0 E ügzee Ürinidveensi t9e1si Fen GFaökrüelvtelsisi iB oiylaorloajki Bçaöllıüşmaüy Zao boalşoljai dAın.abilim Dalı'nca açılan Öğretim Elemanı kadro sınavını kazanarak Araştırma B16az.1ı 2S.i1m9b9i1y'odtei kE .S Üil. iFyeant lBairliımn l(eIrsi oEtnrsitcithüas üs pBpiy.o)l oMjio Arnfoabloiljimik Dvael ıS Zitooollojii kB iYriampiı'lnadreı," " aSdığlıı r Y İüşkkseemk bLeissianndse T Yeazşinaiyan 1ta6m.0a9m.l1a9d9ı. 61'9d9a6, E'd. Üa .K Fuezne Byi lKimıblerrıis E Tnüstritkü sCüu Bmiyhoulroijyi Aetnia Vbailtiman Ddaaşlıl ıZğoı yoalonjıin Bdiar iTmüi'rnkdiey,e " İCşkuemmhbueriyeti Vatandaşı oldu. ESinlityoadtilnairoı mEopridpihniiduam) HCarkakwınledya, 1M9o2r3fo alnodji kO vpeh rTyaoksscoonloexm Sikt eAinra, ş1t8ır5m8 a(lCairl"io apdhlı o rDao:ktora Tezi'ni 0ta1m.1a2m.l1a9ya9r7a'kd eD Eogket oÜrn iuvnervsainteınsiı Falednı. Fakültesi Biyoloji Bölümü Zooloji Anabilim Dalı'nda "Yardımcı Doçent" olarak FÖağkrüetltimes iÜ Byieyloiğloinjie B aötalünmdıü.
    [Show full text]
  • Solenodon Genome Reveals Convergent Evolution of Venom in Eulipotyphlan Mammals
    Solenodon genome reveals convergent evolution of venom in eulipotyphlan mammals Nicholas R. Casewella,1, Daniel Petrasb,c, Daren C. Cardd,e,f, Vivek Suranseg, Alexis M. Mychajliwh,i,j, David Richardsk,l, Ivan Koludarovm, Laura-Oana Albulescua, Julien Slagboomn, Benjamin-Florian Hempelb, Neville M. Ngumk, Rosalind J. Kennerleyo, Jorge L. Broccap, Gareth Whiteleya, Robert A. Harrisona, Fiona M. S. Boltona, Jordan Debonoq, Freek J. Vonkr, Jessica Alföldis, Jeremy Johnsons, Elinor K. Karlssons,t, Kerstin Lindblad-Tohs,u, Ian R. Mellork, Roderich D. Süssmuthb, Bryan G. Fryq, Sanjaya Kuruppuv,w, Wayne C. Hodgsonv, Jeroen Kooln, Todd A. Castoed, Ian Barnesx, Kartik Sunagarg, Eivind A. B. Undheimy,z,aa, and Samuel T. Turveybb aCentre for Snakebite Research & Interventions, Liverpool School of Tropical Medicine, Pembroke Place, L3 5QA Liverpool, United Kingdom; bInstitut für Chemie, Technische Universität Berlin, 10623 Berlin, Germany; cCollaborative Mass Spectrometry Innovation Center, University of California, San Diego, La Jolla, CA 92093; dDepartment of Biology, University of Texas at Arlington, Arlington, TX 76010; eDepartment of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138; fMuseum of Comparative Zoology, Harvard University, Cambridge, MA 02138; gEvolutionary Venomics Lab, Centre for Ecological Sciences, Indian Institute of Science, 560012 Bangalore, India; hDepartment of Biology, Stanford University, Stanford, CA 94305; iDepartment of Rancho La Brea, Natural History Museum of Los Angeles County, Los Angeles,
    [Show full text]
  • Comparative Venomics of the Vipera Ammodytes Transcaucasiana and Vipera Ammodytes Montandoni from Turkey Provides Insights Into Kinship
    toxins Article Comparative Venomics of the Vipera ammodytes transcaucasiana and Vipera ammodytes montandoni from Turkey Provides Insights into Kinship Benjamin-Florian Hempel 1 ID , Maik Damm 1, Bayram Göçmen 2 ID , Mert Karis 2 ID , Mehmet Anıl Oguz 2, Ayse Nalbantsoy 3,* and Roderich D. Süssmuth 1,* 1 Department of Chemistry, Technische Universität Berlin, 10623 Berlin, Germany; [email protected] (B.-F.H.); [email protected] (M.D.) 2 Department of Biology, Ege University, 35100 Izmir, Turkey; [email protected] (B.G.); [email protected] (M. K.); [email protected] (M.A.O.) 3 Department of Bioengineering, Ege University, 35100 Izmir, Turkey * Correspondence: [email protected] (A.N.); [email protected] (R.D.S.) Tel.: +49-30-314-24205 (R.D.S.) Received: 22 September 2017; Accepted: 28 December 2017; Published: 1 January 2018 Abstract: The Nose-horned Viper (Vipera ammodytes) is one of the most widespread and venomous snakes in Europe, which causes high frequent snakebite accidents. The first comprehensive venom characterization of the regional endemic Transcaucasian Nose-horned Viper (Vipera ammodytes transcaucasiana) and the Transdanubian Sand Viper (Vipera ammodytes montandoni) is reported employing a combination of intact mass profiling and bottom-up proteomics. The bottom-up analysis of both subspecies identified the major snake protein families of viper venoms. Furthermore, intact mass profiling revealed the presence of two tripeptidic metalloprotease inhibitors and their precursors. While previous reports applied multivariate analysis techniques to clarify the taxonomic status of the subspecies, an accurate classification of Vipera ammodytes transcaucasiana is still part of the ongoing research.
    [Show full text]
  • Accepted Manuscript
    Accepted Manuscript Intact protein mass spectrometry reveals intraspecies variations in venom composition of a local population of Vipera kaznakovi in Northeastern Turkey Daniel Petras, Benjamin-Florian Hempel, Bayram Göçmen, Mert Karis, Gareth Whiteley, Simon C. Wagstaff, Paul Heiss, Nicholas R. Casewell, Ayse Nalbantsoy, Roderich D. Süssmuth PII: S1874-3919(19)30047-8 DOI: https://doi.org/10.1016/j.jprot.2019.02.004 Reference: JPROT 3323 To appear in: Journal of Proteomics Received date: 20 December 2018 Revised date: 30 January 2019 Accepted date: 10 February 2019 Please cite this article as: D. Petras, B.-F. Hempel, B. Göçmen, et al., Intact protein mass spectrometry reveals intraspecies variations in venom composition of a local population of Vipera kaznakovi in Northeastern Turkey, Journal of Proteomics, https://doi.org/10.1016/ j.jprot.2019.02.004 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ACCEPTED MANUSCRIPT Intact protein mass spectrometry reveals intraspecies variations in venom composition of a local population of Vipera kaznakovi in Northeastern Turkey Daniel Petrasa,b,*,^, Benjamin-Florian Hempela,*, Bayram Göçmenc, Mert Karisc,d, Gareth Whiteleye, Simon C. Wagstafff, Paul Heissa, Nicholas R. Casewelle, Ayse Nalbantsoyg,^ and Roderich D. Süssmutha,^ a Technische Universität Berlin, Institut für Chemie, Strasse des 17.
    [Show full text]
  • Community Venomics Reveals Intra-Species Variations in Venom
    bioRxiv preprint doi: https://doi.org/10.1101/503276; this version posted December 20, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Community venomics reveals intra-species variations in venom 2 composition of a local population of Vipera kaznakovi in Northeastern 3 Turkey 4 5 Daniel Petrasa,b,*,^, Benjamin-Florian Hempela,*, Bayram Göçmenc, Mert Karisc, Gareth Whiteleyd, 6 Simon C. Wagstaffe, Paul Heissa, Nicholas R. Casewelld, Ayse Nalbantsoyf,^ and Roderich D. 7 Süssmutha,^ 8 9 a Technische Universität Berlin, Institut für Chemie, Strasse des 17. Juni 124, 10623 Berlin, Germany. 10 b University of California San Diego, Collaborative Mass Spectrometry Innovation Center, 9500 Gilman Drive, La Jolla, 11 California 92093, United States. 12 c Zoology Section, Department of Biology, Faculty of Science, Ege University, 35100 Bornova, Izmir, Turkey. 13 d Centre for Snakebite Research & Interventions, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, L3 14 5QA, United Kingdom 15 e Bioinformatics Unit, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, L3 5QA, United Kingdom 16 f Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, 35100 Izmir, Turkey. 17 * These authors contributed equally to this study 18 ^ Correspondence to [email protected], [email protected] or [email protected] 19 20 21 Abstract 22 We report on the variable venom composition of a population of the Caucasus viper (Vipera 23 kaznakovi) in Northeastern Turkey.
    [Show full text]
  • (Montivipera Raddei) and Mount Bulgar Viper (Montivipera Bulgardaghica) with Potent Cytotoxicity Against Human A549 Lung Carcinoma Cells
    (This is a sample cover image for this issue. The actual cover is not yet available at this time.) This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the author's institution and sharing with colleagues. Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. In most cases authors are permitted to post their version of the article (e.g. in Word or Tex form) to their personal website or institutional repository. Authors requiring further information regarding Elsevier's archiving and manuscript policies are encouraged to visit: http://www.elsevier.com/authorsrights Author's Personal Copy Toxicon 135 (2017) 71e83 Contents lists available at ScienceDirect Toxicon journal homepage: www.elsevier.com/locate/toxicon Combined venom profiling and cytotoxicity screening of the Radde's mountain viper (Montivipera raddei) and Mount Bulgar Viper (Montivipera bulgardaghica) with potent cytotoxicity against human A549 lung carcinoma cells Ayse Nalbantsoy a, 1, Benjamin-Florian Hempel b, 1, Daniel Petras b, c, Paul Heiss b, * Bayram Goçmen€ d, Nasit Igci e, Mehmet Zülfü Yildiz f, Roderich D. Süssmuth b, a Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, 35100, Izmir, Turkey b Technische Universitat€ Berlin, Institut für Chemie, Strasse des 17. Juni 124, 10623, Berlin, Germany c University
    [Show full text]
  • Tribute to the Late Prof. Dr. Bayram GÖÇMEN (1965–2019)
    NORTH-WESTERN JOURNAL OF ZOOLOGY 15 (2): 201-220 ©NWJZ, Oradea, Romania, 2019 Article No.: e199101 http://biozoojournals.ro/nwjz/index.html Tribute to the late Prof. Dr. Bayram GÖÇMEN (1965–2019) Mehmet Zülfü YILDIZ1*, Gözde GÜRELLİ2, Deniz YALÇINKAYA3, Bahadır AKMAN4, Naşit İĞCİ5,6, Mert KARIŞ7 and Mehmet Anıl OĞUZ8 1. Zoology Section, Department of Biology, Faculty of Sciences and Arts Adıyaman University, Adıyaman, Turkey. 2. Department of Biology, Faculty of Sciences and Arts, Kastamonu University, Kastamonu, Turkey. 3. Medical Laboratory Techniques Program, Department of Medical Services and Techniques, Vocational School, Toros University, Mersin, Turkey. 4. Hunting and Wildlife Program, Department of Forestry, Vocational School of Technical Sciences, Iğdır University, Iğdır, Turkey. 5. Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Nevşehir Hacı Bektaş Veli University, Nevşehir, Turkey. 6. Science and Technology Application and Research Center, Nevşehir Hacı Bektaş Veli University, Nevşehir, Turkey. 7. Program of Laboratory Technology, Department of Chemistry and Chemical Process Technologies, Acigöl Vocational School of Technical Sciences, Nevşehir Hacı Bektaş Veli University, Nevşehir, Turkey. 8. Zoology Section, Department of Biology, Faculty of Science, Ege University, Izmir, Turkey. Abstract. If there was something written to my destiny: "Biology / Zoology is not a profession for me, it is my lifestyle. My feelings, approaches, and reactions are coming out of this lifestyle. If you want to learn something, experience it! If you take nature as an example, there is no chance of making a mistake! That is the right way!" ‒ Prof. Dr. Bayram GÖÇMEN. We (and many people around the world who know him) are in deep sorrow to have lost Prof.
    [Show full text]