COVER Recently, nanotechnology for biomedical research has received extensive attention, and considerable progress has been made in this area. Nanotechnology encompasses many advanced research areas such as nanocharacterization and nanomeasurement based on optoelectronic technology, nanomaterials with optically modulated parameters, nanophotonics and nano-optoelectronics. These research areas cover important applications such as detection and manipulation of bionanostructures, in vitro and in vivo characterization of biomolecules for clinical diagnosis, imaging and detection of single cells, cell masses and tissues, functional nanomaterials for dietetic therapy, and nanoscale medical transportation systems. As a result, nano-optoelectronics for biomedical research has developed into an important and highly promising interdisciplinary field. The background photograph on the cover shows in vivo non-invasive NIR fluorescence images of nano-drug carriers. Targeting molecules favors the in vivo distribution of drug molecules in the target region, which improves therapeutic efficiency (see the special issue: Nano-Biomedical Optoelectronic Materials and Devices).

Volume 58 Number 21 July 2013

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Abstracted/indexed in: Academic Search Alumni Edition EMBio Academic Search Complete Environmental Engineering Abstracts Academic Search Elite Environmental Sciences and Pollution Management Academic Search Premier Google Scholar ASFA 1: Biological Sciences and Living Resources Inspec ASFA 2: Ocean Technology, Policy and Non-Living Resources Mathematical Reviews Biological Abstracts MathSciNet Biological Sciences Meteorological and Geoastrophysical Abstracts BIOSIS Previews Pollution Abstracts CAB Abstracts Science Citation Index Chemical Abstracts SCOPUS Chemical and Earth Sciences Water Resources Abstracts Current Contents/Physical Zentralblatt MATH Current Mathematical Publications Zoological Record Digital Mathematics Registry CONTENTS Volume 58 Number 21 July 2013 CONTENTS

SPECIAL ISSUE: Nano-Biomedical Optoelectronic Materials and Devices

REVIEWS 2521 Nano-opto-electronics for biomedicine GU Ning, LI Yan, WANG Meng & CAO Min

2530 Applications of gold nanorods in biomedical imaging and related fields MA ZhiYa, XIA HongXing, LIU YuPing, LIU Bo, CHEN Wei & ZHAO YuanDi

2537 Nano-bio interfaces probed by advanced optical spectroscopy: From model system studies to optical biosensors ZHANG XiaoXian, HAN XiaoFeng, WU FuGen, JASENSKY Joshua & CHEN Zhan

2557 Beyond displays: The recent progress of liquid crystals for bio/chemical detections DONG YuanChen & YANG ZhongQiang

2563 Nanostructured ZnO for biosensing applications XU ChunXiang, YANG Chi, GU BaoXiang & FANG ShengJiang

LETTER 2567 Rapid detection of Cyfra 21-1 by optical-biosensing based on chemiluminescence immunoassay using bio-functionlized magnetic nanocomposites LUO JinPing, QU ShuXue, LIU JunTao, WANG Bin & CAI XinXia

ARTICLES

2570 Star-shaped conjugated oligoelectrolyte for bioimaging in living cells SONG WenLi, JIANG RongCui, YUAN Yan, LU XiaoMei, HU WenBo, FAN QuLi & HUANG Wei

2576 In vitro assay of cytoskeleton nanomechanics as a tool for screening potential anticancer effects of natural plant extract, tubeimoside I on human hepatoma (HepG2) cells ZHAO HongBo, WANG YaShu, JIANG XueMei, SHI XiaoHao, ZHONG HongZhe, WANG YaJie, CHEN Jun & DENG LinHong

2584 Cinobufacini-induced HeLa cell apoptosis enhanced by curcumin LIU Li, JIN Hua, OU JinLai, JIANG JinHuan, PI Jiang, KE ChangHong, YANG Fen, QIAO DongJuan, CAI HuaiHong & CAI JiYe

2594 Single-cell discrimination based on optical tweezers Raman spectroscopy MA HongFei, ZHANG Yong & YE AnPei

2601 AlGaN/GaN heterostructure field transistor for label-free detection of DNA hybridization WEN XueJin, WANG ShengNian, WANG YuJi, LEE Ly James & LU Wu

2606 Selectable infiltrating large hollow core photonic band-gap fiber LIU JianGuo, DU YuanXin, ZHU NingHua & LIU FengMei

2611 High-sensitive and temperature-self-calibrated tilted fiber grating biological sensing probe LIU Fu, GUO Tuan, LIU JianGuo, ZHU XiaoYang, LIU Yu, GUAN BaiOu & ALBERT Jacques

2616 Effect of protein molecules on the photoluminescence properties and stability of water-soluble CdSe/ZnS core-shell quantum dots ZHANG YouLin, TU LangPing, ZENG QingHui & KONG XiangGui

2622 Optical detection of acetylcholine esterase based on CdTe quantum dots CHEN ZhenZhen, REN XiangLing & TANG FangQiong

2628 Tween-modified suspension array for sensitive biomolecular detection ZHAO Zhuan, LI Zong & LI Jiong

: Progress of Projects Supported by NSFC

www.scichina.com | csb.scichina.com | www.springer.com/scp | www.springerlink.com i CONTENTS CONTENTS

2634 Sensitive monitoring of RNA transcription levels using a graphene oxide fluorescence switch ZHOU XiaoMing, LIAO YuHui & XING Da

2640 A multiple-labelling method for cells using Au nanoparticles with different shapes ZHANG Ke, FENG JianTao, SUN QuanMei, JIN Lin, LI Jing, WU XiaoChun & HAN Dong

2646 Programmed self-assembly of DNA origami nanoblocks into anisotropic higher-order nanopatterns FU YanMing, CHAO Jie, LIU HuaJie & FAN ChunHai

2651 p-Hydroxybenzoic acid (p-HA) modified polymeric micelles for brain-targeted docetaxel delivery ZHANG ZhiXin, WEI XiaoLi, ZHANG XiaoYu & LU WeiYue

2657 Protease-activated quantum dot probes based on fluorescence resonance energy transfer LI Xin, XUE Bing, LI Yang & GU YueQing

2663 Generalized multiparticle Mie modeling of light scattering by cells WANG Meng, CAO Min, GUO ZhiRui & GU Ning

Vol. 58 No. 21 July 25, 2013 (Published three times every month)

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SPECIAL ISSUE: Nano-Biomedical Optoelectronic Materials and Devices July 2013 Vol.58 No.21: 26342639 doi: 10.1007/s11434-012-5584-2

Sensitive monitoring of RNA transcription levels using a graphene oxide fluorescence switch

ZHOU XiaoMing, LIAO YuHui & XING Da*

Key Laboratory of Laser Life Science & Institute of Laser Life Science (Ministry of Education), College of Biophotonics, South China Normal University, Guangzhou 510631, China

Received July 18, 2012; accepted October 26, 2012; published online December 26, 2012

Catalytic transfer of genetic information from DNA to RNA is very important in life activities. The unconventional RNA tran- scription level may be related to the source of genetic diseases. At present, conventional methods for detection of RNA transcripts usually involve cumbersome preparative steps, or require sophisticated laboratory equipments. In this study, we presented a rapid, sensitive nano-detection platform for monitoring of RNA transcript levels. T7 RNA polymerase transcription reaction is employed as the example to test the feasibility of this method. In this design, in vitro synthesized RNA products can be hybridized to the FAM labeled single strand DNA (ssDNA) probes which can be adsorbed onto the graphene oxide (GO) surface. Using GO as the fluorescence switch, excellent capacity of the signal-on fluorescence platform for detection of RNA transcripts level is demon- strated. Transcription levels sensing with this nano-platform achieved a sensitivity of 5 pmol/L transcription template. It is antici- pated that current developed RNA transcript nano-detection mode has the potential to be an alternative to the conventional RNA transcript detection methods. nanobiotechnology, graphene oxide, fluorescence resonance energy transfer, RNA transcription level

Citation: Zhou X M, Liao Y H, Xing D. Sensitive monitoring of RNA transcription levels using a graphene oxide fluorescence switch. Chin Sci Bull, 2013, 58: 26342639, doi: 10.1007/s11434-012-5584-2

Catalytic transfer of genetic information from DNA to RNA tion, thus requires strict requirements for laboratory equip- is one of the most grounded life processes [1]. In the tran- ment and the environment, which may lead to the poor ex- scription reaction, DNA is used as the template, and the perimental reproducibility. Reverse transcription-PCR method genetic information is passed to the RNA genome. RNA is relatively simple and sensitive. However, electrophoresis transcription is the genetic basis of many important in vivo detection takes a long time and need to use toxic reagents. life activities. This is because abnormal expression of genes Microarray and in situ hybridization method requires so- usually caused protein dysfunction. At present, it is believed phisticated equipment, and is not conducive to the adoption that the transcription levels of gene expression profiles from and using of the technology. Another potential application the mRNA levels can reflect the activities of abnormal cells of the transcription levels detection is that isothermal tran- in early disease development [2–7]. scription of the nucleic acid can be used as a signal ampli- At present, methods for detection of RNA transcripts fication platform [8]. By the conjugation of nucleic acids level include Northern blot, dot blot, reverse transcription and antibody, the linked nucleic acid can be transcribed into polymerase chain reaction (RT-PCR), microarray, in situ thousands of single strand RNA molecules. Through the hybridization method, and so on. Northern blot and dot blot detection of transcription products, more than one thousand involve cumbersome experiment steps during the operation. times signal amplification can be achieved in protein detec- Moreover, RNA is susceptible to the RNA enzyme degrada- tion [8,9]. Recently, some high-sensitivity biological analy- sis tools were developed based on transcription mediated *Corresponding author (email: [email protected]) amplification [8–15].

© The Author(s) 2012. This article is published with open access at Springerlink.com csb.scichina.com www.springer.com/scp Zhou X M, et al. Chin Sci Bull July (2013) Vol.58 No.21 2635

For these reasons, the development of new detection from Xianfeng Nanotechnologies Co., Ltd (Nanjing, China). methods for the transcriptional level will play a vital role in T7 RNA polymerase and ribonucleotide triphosphate (rNTPs) the interpretation of specific functional gene regulation mix were the products of New England Biolabs (NEB). mechanisms, the elucidation of molecular basis of disease SYBR I and SYBR II were purchased from Invitrogen. All caused by gene expression abnormalities, and the drug tar- oligonucleotides and probes used in our research are syn- get discovery and high throughput drug screening. Further- thesized and purified by HPLC at Shanghai Sangon Biolog- more, novel transcriptional levels monitoring method can ical Engineering and Technology & Services Co., Ltd. The also provide high sensitivity analytical tool for bio-sensing regents related to electrophoresis were purchased from at the single molecule level [16]. Bio-Rad (Richmond, CA, USA). SSC buffer and RNase- With the development of nanotechnology, nanomaterial free water were purchased from Shanghai Sangon Biotech- has become an important tool in biotechnology research. In nology Co. Ltd. recent years, due to its excellent physical and chemical (2) Apparatus. Perkin-Elmer LS55 luminescence spec- properties, graphene oxide (GO) has gained tremendous trometer (USA); fluorescence microplate reader (Infinite interests in the biosensor fields for biosensors building. GO M200, TECAN); slab electrophoresis system and imaging is constituted by single-layer sheet structure carbon atoms system (Bio-Rad). [17–22], with carboxyl and phenol hydroxyl group on its surface. GO has a net negative charge, good dispersion, and 1.2 T7 RNA transcription system excellent stability [20]. It is also well known that single- stranded nucleic acid is soft and loose linear structure, thus The transcription system contained DNA template, rNTPs adsorption of it onto the GO is stable, due to - stacking mix (2 mmol/L), T7 RNA polymerase (2.5 U/L), 1× effect between the nucleic acid base and GO [23,24]. How- corresponding buffer, and ribonuclease inhibitor (1 U/L). ever, the formation of the double helix nucleic acid structure The DNA template was two single strands obtained by gra- is rigid, therefore greatly reduces its adsorption capacity by dient cooling (denatured at 95°C for 5 min, then cooling to the GO [23,24]. On the other hand, one interesting property 25°C, 2°C per min). Their sequences were 5′-CGCGAA- of GO is that it can be used as a broad-spectrum fluores- ATTAATACGACTCACTATAGGGAGA-3′ (coding strand) cence quenching agent [23–29]. Therefore, fluorophores and 5′-AACTTTCAACATCAGTCTGATAAGCTATCT- labeled in single-stranded nucleic acid probe can be effec- CCCTATAGTGAGTCGTATTAATTTCGCG-3′ (template tively quenched by GO through fluorescence resonance strand). The transcription mixture was incubated for 2 h at energy transfer (FRET) mechanism. This distinct function 37°C. derived from its heterogeneous chemical atomic structure and electronic properties, and the sp2 hybrid crystal domain of GO is believed to play leading role in fluorescence 1.3 Polyacrylamide gel electrophoresis quenching [17]. This quenching effect can be used as a flu- Transcription products were analyzed on a Bio-Rad (Bio- orescence switch, thereby enhancing the signal to noise ra- Rad Laboratories, USA) slab electrophoresis system. The tio of biological sensing. Based on this principle, a series of graphene-based bimolecular sensing technologies have been 10 L samples were loaded onto a 10% native polyacryla- developed in recent years [23–29]. mide gel (29:1, acryl:bisacryl) in 0.5×Tris-borate-EDTA The utilization of GO allows the attachment of the func- (TBE). Gels were run at room temperature for 1 h at 120 V. tional nucleic acids probe onto its surface, thus providing the The gel was confirmed by SYBR I and SYBR II staining opportunity to construct a new strategy to detect RNA tran- and photographed by Bio-Rad digital imaging system. scription levels. In this design, fluorophore labeled in the DNA probe acting as the energy donor and by functioning 1.4 GO nano-detection platform GO as the energy acceptor. Here, we chose T7 RNA polymer- ase transcription reaction to test the feasibility of this method. The steps were as follows: the single-stranded RNA product The results demonstrated that the GO fluorescence switch from transcription was dissolved in 1×SSC buffer and FAM can be an excellent and versatile transcription monitoring plat- probe was also added to make a final concentration of form. It is anticipated that currently developed RNA transcript 50 nmol. The resulting mixture was incubated for 30 min at nano-detection modes have the potential to be an alternative 37°C, and then FAM probe would hybridize with RNA to the conventional RNA transcript detection methods. product. Afterwards, GO was added to make a final concen- tration of 8 g/mL. After incubating for 10 min, the fluo- rescence was measured with luminescence spectrometer. 1 Materials and methods The sequence of FAM probe was 5′-TTTCAACATCAG- 1.1 Reagents and apparatus TCTGATAAGCTATCTCCC-3′, and the 3′ terminal was labeled with FAM fluorescent (excitation at 488 nm and (1) Reagents. Graphene oxide (1 mg/mL) was purchased emission at 520 nm). 2636 Zhou X M, et al. Chin Sci Bull July (2013) Vol.58 No.21

2 Results and discussions In order to verify the validity of the T7 transcription re- action, 50 nmol/L DNA template is transcribed by T7 RNA 2.1 Design of the GO fluorescence switch strategy polymerase. The transcripts were detected using 12% poly- acrylamide gel electrophoresis. Figure 2 shows that the This study aims to build a fast, sensitive detection tool for RNA transcripts can be stably produced from three paral- RNA transcript levels with the GO fluorescent switch. The leled transcription experiments. platform is based on nucleic acid hybridization and FRET principle. We used T7 RNA polymerase transcript reaction as the example to test the feasibility. T7 RNA polymerase, 2.2 Parameters for graphene fluorescence switch encoded by T7 bacteriophage [30,31], is highly specific to detection of transcripts the promoter sequence (TAATACGACTCACTATAGGG- In building process of this nano-detection platform, it is AGA). The enzyme is widely used in the isothermal ampli- found that the RNA products of transcription system can be fication of nucleic acids. In T7 RNA polymerase transcrip- adsorbed onto GO surface at high GO concentration, lead- tion, double-stranded template DNA is necessary for the ing to desensitization of the nano-detection platform. How- promotor region, but the transcription region only needs the ever, when GO concentration is too low, single-stranded single strand as the template. Therefore, in this study single- FAM probe cannot be completed adsorbed, resulting in a stranded template strand is employed in the transcribed re- strong background fluorescence signal, thus to lower the gion. The experimental principle is shown in Figure 1. First, signal to noise ratio. To get this optimized parameter, ex- T7 RNA polymerase binds to the promotor region of the periments were executed to evaluate the effect of GO con- template. Adding of the rNTPs leads to the continuous centration on the fluorescence quenching ability. FAM la- transcription. A FAM labeled DNA probe was designed to beled DNA probe concentration was fixed for 50 nmol/L in hybridize to the corresponding single-stranded RNA tran- all experimental groups. GO concentrations are 2, 4, 6, 8, 10 scripts. GO has been demonstrated interacting strongly with g/mL, respectively. As shown in Figure 3, with the in- nucleotides through -stacking interaction. However, ad- crease of the GO concentration, fluorescence quenching sorption of double stranded DNA (dsDNA) onto the GO efficiency got increased, indicating that more FAM labeled surface is weak, due to the shielding of nucleobases within DNA probe was adsorbed. When the GO concentration was the negatively charged phosphate backbone of dsDNA. increased to 8 g/mL, fluorescence quenching efficiency Furthermore, GO can quench the fluorescence of the nearby achieved a maximal value. Further increasing that the GO dyes. By monitoring the increase in fluorescence intensity, concentration quenching efficiency was not significantly the dynamics of transcription reaction can be detected with improved. Therefore, it seems 8 g/mL GO is the optimum very high sensitivity. choice for the subsequent nano-detection platform.

Figure 1 The principle of monitoring of RNA transcription levels using a GO fluorescence switch. Zhou X M, et al. Chin Sci Bull July (2013) Vol.58 No.21 2637

keeps stabilized. Therefore, 8 min was chosen as optimal adsorption time in subsequent experiments.

2.3 Sensitivity results and data analysis

In order to verify the sensitivity of GO nano-detection plat- form for RNA transcripts, transcription templates sensitivity experiments were designed. DNA transcription template was varied from the concentration of 0.5, 5, 50, 500 pmol/L, 5 nmol/L, to 50 nmol/L, respectively. Transcription time was 2 h. Transcripts derived from different template con- centrations were monitored by GO nano-detection platform. Results are shown in Figure 5. It is found that fluorescence intensity gradually increased with the increasing concentra- tion of DNA transcription template. Further increase of the concentration of the DNA transcription template to 5 nmol/L did not significantly enhance the fluorescence intensity, Figure 2 Electrophoresis identification of the T7 transcription reaction. The transcription products are separated by 12% polyacrylamide gel elec- suggesting that 5 nmol/L DNA template has reached the trophoresis. DNA is screened with a SYBR GREEN I/II MIX staining saturation limit of the GO nano-platform testing. When the method. Lanes 1 to 3 represent the three repeated experiments. concentration of DNA transcription template is 0.5 pmol/L, similar fluorescence intensities are observed between ex- perimental group and control group. However, with 5 pmol/L transcription template in the transcription and detection sys- tem, the fluorescence intensity was significantly enhanced. Because more detailed transcription template concentration experiments are not executed, it is well demonstrated that current developed GO nano-detection platform achieved a sensitivity of 5 pmol/L. In order to verify the reliability of experimental data and the feasibility of this nanotechnology platform for quantita- tively testing RNA transcription levels, a histogram is con- structed and linear dependence of the fluorescence intensi- ties of different DNA template concentrations was analyzed based on repeated experiments and statistics. The results are shown in Figure 5. A R2 value of 0.967 was obtained from

Figure 3 Evaluation of the effect of GO concentration on the fluores- cence quenching ability. Optimal GO concentration is 8 g/mL.

On the other hand, time dynamics of fluorescent probes adsorption is also an important parameter. It is because single- chain fluorescent probes could not be effectively adsorbed onto GO if too short adsorption time is employed. However, random adsorption of double-stranded hybrid products may occur when long incubation time is used, which reduces the signal to noise ratio. In order to improve the detection effi- ciency of the nano-detection platform, the GO adsorption time optimization experiments were executed. FAM labeled DNA probe concentration is fixed at 50 nmol/L, GO concen- tration is 8 g/mL, and adsorption time variable detection of the fluorescence intensity is observed at different time point. The experimental results are shown in Figure 4. It is found that with the increase of adsorption time, the fluorescence Figure 4 Time dynamics of fluorescent probes adsorption. GO concen- quenching efficiency gradually increased. When the adsorp- tration is 8 g/mL, FAM labeled DNA probe concentration was fixed for tion time was 8 min, the fluorescence quenching efficiency 50 nmol/L. 2638 Zhou X M, et al. Chin Sci Bull July (2013) Vol.58 No.21

Figure 5 Fluorescence spectroscopy experiments for measuring transcripts levels derived from different concentrations of DNA transcription template. GO concentration is 8 g/mL, FAM labeled DNA probe concentration was fixed for 50 nmol/L. Incubation time is 8 min. the concentration of 5 pmol/L to 5 nmol/L. Based on this, it To the best of our knowledge, it is the first reported ex- is well demonstrated the feasibility and reliability of the ample of exploring nanomaterial for the transcripts levels platform used for the detection of RNA transcript levels. monitoring. However, it is obvious that current strategy is not limited to the sensing of RNA transcription levels. One of the ideational examples is the sensing of the nucleic acid 3 Conclusions sequence based amplification (NASBA) reaction, which is an isothermal and sensitive nucleic acids detection technol- In summary, it is demonstrated that an approach can be po- ogy having plenty of applications. tentially applied to the detection of RNA transcript levels. The proposed method is based on a GO fluorescence switch made of one dye labeled single stranded DNA obtained This work was supported by the National Basic Research Program of Chi- from conventional chemical synthesis and GO nanomaterial. na (2010CB732602), the Key Program of NSFC-Guangdong Joint Funds of China (U0931005), the National Natural Science Foundation of China Since GO is an excellent and broad spectrum fluorescence- (81101121) and the Natural Science Foundation of Guangdong Province quencher, using GO as the fluorescence detection platform (S2011040005386). is an attractive option. The strategy to sensing RNA tran- script levels with a dye-labeled DNA probe by hybridization also circumvents the processes of chemical modification 1 Blake W J, Kaern M, Cantor C R, et al. Noise in eukaryotic gene expression. Nature, 2003, 422: 633–637 and conjugation of GO. 2 Bachl J, Carlson C, Schopfer V G, et al. Increased transcription levels Three remarkable advantages can be concluded when induce higher mutation rates in a hypermutating cell line. J Immunol, compared to some conventional assays. Firstly, the whole 2001, 166: 5051–5057 detection process is rapid. The 10-min incubation time is 3 Rahman I, MacNee W. Regulation of redox glutathione levels and gene transcription in lung inflammation: Therapeutic approaches. enough for effective hybridization of dye labeled DNA Free Radic Biol Med, 2000, 28: 1405–1420 probes and RNA transcripts. Secondly, RNA transcripts 4 Nagalakshmi U, Wang Z, Waern K, et al. The transcriptional land- from 5 pmol/L DNA template can be reliably detected, sug- scape of the yeast genome defined by RNA sequencing. Science, 2008, gesting that current assay can be a robust tool for transcrip- 320: 1344–1349 5 Pugliese A, Zeller M, Jr A F, et al. The insulin gene is transcribed in tion-mediated amplification based bioassays. Thirdly, alt- the human thymus and transcription levels correlate with allelic vari- hough single dye labeled probe is used for monitoring the ation at the INS VNTR-IDDM2 susceptibility locus for type 1 diabe- RNA transcripts, the method has the potential to be a mul- tes. Nat Genet, 1997, 15: 293–297 ticolor fluorescent RNA transcripts analysis. By employing 6 Terry C F, Loukaci V, Green F R. Cooperative influence of genetic polymorphisms on interleukin 6 transcriptional regulation. J Biol of different dye labeled DNA probes, different RNA tran- Chem, 2000, 275: 18138–18144 scripts can be detected instantaneously in single tube reaction. 7 Jenssen T K, Lægreid A, Komorowski J, et al. A literature network of Zhou X M, et al. Chin Sci Bull July (2013) Vol.58 No.21 2639

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Honorary Editor General: ZHOU GuangZhao (Zhou Guang Zhao) Editor General: ZHU ZuoYan Editor-in-Chief: XIA JianBai

Executive Associate Editors-in-Chief Physics, Mechanics and Astronomy CHEN DongMin CHEN Hong GONG QiHuang LONG GuiLu Chemistry BAO XinHe JIANG GuiBin LI YaDong XI ZhenFeng Life Sciences MA KePing TIAN ZhiGang WU JiaRui WU WeiHua ZHAN QiMin Earth Sciences GUO ZhengTang NIU YaoLing ZHENG YongFei Materials and Engineering Sciences TAO WenQuan WANG LianZhou XU NingSheng Information Sciences ZHANG Ping ZHENG NanNing ZHOU ZhiHua

Members Physics, Mechanics and Astronomy CHEN NuoFu CHEN WeiQiu CHEN XianFeng CHEN YanFeng CHEN ZhangHai CHEN ZhiJian DENG YuanYong DING DaJun FAN Jing FANG Jing GONG XinGao GUO GuangCan HOU MeiYing HU An HU GengKai HUANG Tao JI PeiWen JIANG ZuiMin JIN ChangQing KANG YiLan LAN YueHeng LI RuXin LI Yan LI YouQuan LI ZhiYuan LIAO XinHao LING GuoCan LIU JiXing LIU YunQuan LÜ Rong LU Wei LUO QingMing MA YuanLiang NING JianGuo PENG LianMao QIAO GuoJun SHAO ZhiFeng SUN Yang TIAN Lin TONG DianMin TONG LiMin WANG HuiTian WANG JiYang WANG PengYe WANG Wei WANG WeiHua WANG YuPeng WEI BaoWen WEI JianYan WU DeJin WU Ying WU YueLiang XIAO Min XU FuRong XU HongXing XUE Ping YANG Lan ZENG HePing ZHAN QiWen ZHANG HaiLan ZHANG TianCai ZHANG TianJue ZHANG WeiYi ZHANG ZhiGang ZHAO ZhenTang ZHENG DongNing ZHENG QuanShui ZHONG DongPing ZHU JinSong

Chemistry BO ZhiShan BU XianHe CAI Yong CAI ZongWei CHE ShunAi CHEN JingWen CHEN XiaoYuan (Shawn) CHEN ZhongNing DENG Feng DU Walter DUAN XiangFeng GAN LiangBing GAO ChangYou GAO MingYuan GONG LiuZhu GUO LiangHong GUO ZiJian HAN BuXing HAN KeLi HAN YanChun JIANG DongLin JIN GuoXin LE X Chris LI XiangDong LI Yan LI YanMei LIANG WenPing LIU ChunZhao LIU Yu LIU ZhengPing LIU ZhenYu LIU ZhiPan MA DaWei MA HuiMin MAO BingWei PANG Dai-Wen QI LiMin SHAO YuanHua SHEN WenJie SU ChengYong SU HongMei SU ZhongMin TANG Yi TANG Yong WAN XinHua WANG GuanWu WANG QiuQuan WANG Xun WANG Ye WU Kai WU LiZhu XI Zhen XIA XingHua YANG Bai YANG JinLong YU HanQing YU JiHong YU ShuHong ZHANG DeQing ZHOU BingSheng ZHU BenZhan ZHU LiZhong ZOU HanFa

Life Sciences CHEN GuoQiang CHEN Yan CHENG ZhuKuan CHONG Kang FAN SiLu FANG ShengGuo FU XiangDong FU XiaoLan GONG ZhiZhong GUAN YouFei HAN ZeGuang HU ZhiHong HUANG DaFang HUANG Li HUANG ShuangQuan JIANG ZhiGang LIN JinXing LIU ZhiHua LU BaoRong MA DaLong PEI DuanQing QIN Song QIU XiaoBo QU ChunFeng QU LiangHu QU LiJia SHA JiaHao SHAO RongGuang SHI SuHua SUN MengXiang TAN RenXiang WAN JianMin WAN ShiQiang WANG Gang WANG JianWei WANG YiPing WU Mian WU WeiRen XU Tao XU XuDong XUE YongBiao YANG HongQuan YU JiaLin ZHANG ChuanMao ZHANG LiXin ZHANG Xu ZHANG Xue ZHANG XueMin ZHANG YaPing ZHONG Yang ZHOU PingKun ZUO JianRu

Earth Sciences CHAI YuCheng CHEN FaHu CHEN HongBin CHEN Ling CHENG Hai DAI MinHan DONG YunShe FANG XiaoMin FENG XueShang FU SuiYan GAO Shan GAO Shu GAO Xing GENG AnSong GONG Peng JIAN ZhiMin JIANG ShaoYong LI JianCheng LI ShengHua LI Xia LI XianHua LI Yan LIU KamBiu LIU LiBo LIU XiaoDong LÜ HouYuan LU HuaYu LU QuanMing LUO YongMing MA JianZhong MENG Jin PAN YongXin PENG PingAn SHAO XueMei SHEN YanAn WANG DongXiao WANG HuiJun WANG RuCheng WANG Yang WANG YueJun WU DeXing WU FuYuan XIAO JüLe XIAO WenJiao XIE ShuCheng XU YiGang YANG JinHui YE Kai YU ZiCheng YUAN XunLai ZHANG HongFu ZHANG LiFei ZHANG PeiZhen ZHANG RenHe ZHANG XiaoYe ZHANG ZhongJie ZHAO DaPeng ZHAO GuoChun ZHENG HongBo ZHENG JianPing ZHOU GuangSheng ZHOU LiPing ZHOU ZhongHe ZHU MaoYan

Materials and Engineering Sciences CHEN XianHui CHEN XiaoDong CHENG HuiMing DAI LiMing DING Han DING JianDong GAO RuiPing HE YaLing HU XiJun JIN HongGuang LEUNG Dennis Y C LI YanRong LI YongXiang LÜ ZhaoPing LUO JianBin NAN CeWen NI JinRen SHI JianLin SU WanHua SUN HongBo SUN Jun SUN YuanZhang SUNDELL Jan WANG RuZhu Wang XiaoLin WANG ZhongLin WEI BingBo XU ChunXiang YANG GuoWei YAO KeFu YU AiBing YU DaPeng YU XiPing ZHANG Di ZHANG Xing ZHANG YinPing ZHANG ZheFeng ZHENG ChuGuang ZHU XianFang

Information Sciences FENG DengGuo GAO JingHuai HAN WenBao HONG Wei HU DeWen HU ZhanYi JIANG XiaoYi LI CuiHua LI ShaoQian LIU Min LIU YuLiang LIU ZhiYong LU Jian LU ZuHong MEI Hong NI Lionel M TAN Min WAN MingXi WANG FeiYue WANG HuaiMin WANG Jing WANG Jue WANG XiaoYun XU YangSheng YAO Xin YIN QinYe ZHANG Rong ZHANG Xing ZHANG XueGong ZHAO JiZhong ZHU NingHua ZHU ShiHua ZHUANG YueTing