Sensitive Monitoring of RNA Transcription Levels Using a Graphene Oxide Fluorescence Switch ZHOU Xiaoming, LIAO Yuhui & XING Da
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COVER Recently, nanotechnology for biomedical research has received extensive attention, and considerable progress has been made in this area. Nanotechnology encompasses many advanced research areas such as nanocharacterization and nanomeasurement based on optoelectronic technology, nanomaterials with optically modulated parameters, nanophotonics and nano-optoelectronics. These research areas cover important applications such as detection and manipulation of bionanostructures, in vitro and in vivo characterization of biomolecules for clinical diagnosis, imaging and detection of single cells, cell masses and tissues, functional nanomaterials for dietetic therapy, and nanoscale medical transportation systems. As a result, nano-optoelectronics for biomedical research has developed into an important and highly promising interdisciplinary field. The background photograph on the cover shows in vivo non-invasive NIR fluorescence images of nano-drug carriers. Targeting molecules favors the in vivo distribution of drug molecules in the target region, which improves therapeutic efficiency (see the special issue: Nano-Biomedical Optoelectronic Materials and Devices). Volume 58 Number 21 July 2013 Journal Ownership by Science China Press; Copyright of Articles: © The Author(s) 2013 Journal’s Policy for Open Access All articles published in the journal Chinese Science Bulletin are subject to the Creative Commons Attribution License (http:// creativecommons.org/licenses/by/2.0/). Publishing an article with open access leaves the copyright with the author and allows user to read, copy, distribute and make derivative works from the material, as long as the author of the original work is cited. 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Copyright and License Agreement For copyright regulations and license agreement, please go to www.springeropen.com/about/copyright Abstracted/indexed in: Academic Search Alumni Edition EMBio Academic Search Complete Environmental Engineering Abstracts Academic Search Elite Environmental Sciences and Pollution Management Academic Search Premier Google Scholar ASFA 1: Biological Sciences and Living Resources Inspec ASFA 2: Ocean Technology, Policy and Non-Living Resources Mathematical Reviews Biological Abstracts MathSciNet Biological Sciences Meteorological and Geoastrophysical Abstracts BIOSIS Previews Pollution Abstracts CAB Abstracts Science Citation Index Chemical Abstracts SCOPUS Chemical and Earth Sciences Water Resources Abstracts Current Contents/Physical Zentralblatt MATH Current Mathematical Publications Zoological Record Digital Mathematics Registry CONTENTS Volume 58 Number 21 July 2013 CONTENTS SPECIAL ISSUE: Nano-Biomedical Optoelectronic Materials and Devices REVIEWS 2521 Nano-opto-electronics for biomedicine GU Ning, LI Yan, WANG Meng & CAO Min 2530 Applications of gold nanorods in biomedical imaging and related fields MA ZhiYa, XIA HongXing, LIU YuPing, LIU Bo, CHEN Wei & ZHAO YuanDi 2537 Nano-bio interfaces probed by advanced optical spectroscopy: From model system studies to optical biosensors ZHANG XiaoXian, HAN XiaoFeng, WU FuGen, JASENSKY Joshua & CHEN Zhan 2557 Beyond displays: The recent progress of liquid crystals for bio/chemical detections DONG YuanChen & YANG ZhongQiang 2563 Nanostructured ZnO for biosensing applications XU ChunXiang, YANG Chi, GU BaoXiang & FANG ShengJiang LETTER 2567 Rapid detection of Cyfra 21-1 by optical-biosensing based on chemiluminescence immunoassay using bio-functionlized magnetic nanocomposites LUO JinPing, QU ShuXue, LIU JunTao, WANG Bin & CAI XinXia ARTICLES 2570 Star-shaped conjugated oligoelectrolyte for bioimaging in living cells SONG WenLi, JIANG RongCui, YUAN Yan, LU XiaoMei, HU WenBo, FAN QuLi & HUANG Wei 2576 In vitro assay of cytoskeleton nanomechanics as a tool for screening potential anticancer effects of natural plant extract, tubeimoside I on human hepatoma (HepG2) cells ZHAO HongBo, WANG YaShu, JIANG XueMei, SHI XiaoHao, ZHONG HongZhe, WANG YaJie, CHEN Jun & DENG LinHong 2584 Cinobufacini-induced HeLa cell apoptosis enhanced by curcumin LIU Li, JIN Hua, OU JinLai, JIANG JinHuan, PI Jiang, KE ChangHong, YANG Fen, QIAO DongJuan, CAI HuaiHong & CAI JiYe 2594 Single-cell discrimination based on optical tweezers Raman spectroscopy MA HongFei, ZHANG Yong & YE AnPei 2601 AlGaN/GaN heterostructure field transistor for label-free detection of DNA hybridization WEN XueJin, WANG ShengNian, WANG YuJi, LEE Ly James & LU Wu 2606 Selectable infiltrating large hollow core photonic band-gap fiber LIU JianGuo, DU YuanXin, ZHU NingHua & LIU FengMei 2611 High-sensitive and temperature-self-calibrated tilted fiber grating biological sensing probe LIU Fu, GUO Tuan, LIU JianGuo, ZHU XiaoYang, LIU Yu, GUAN BaiOu & ALBERT Jacques 2616 Effect of protein molecules on the photoluminescence properties and stability of water-soluble CdSe/ZnS core-shell quantum dots ZHANG YouLin, TU LangPing, ZENG QingHui & KONG XiangGui 2622 Optical detection of acetylcholine esterase based on CdTe quantum dots CHEN ZhenZhen, REN XiangLing & TANG FangQiong 2628 Tween-modified suspension array for sensitive biomolecular detection ZHAO Zhuan, LI Zong & LI Jiong : Progress of Projects Supported by NSFC www.scichina.com | csb.scichina.com | www.springer.com/scp | www.springerlink.com i CONTENTS CONTENTS 2634 Sensitive monitoring of RNA transcription levels using a graphene oxide fluorescence switch ZHOU XiaoMing, LIAO YuHui & XING Da 2640 A multiple-labelling method for cells using Au nanoparticles with different shapes ZHANG Ke, FENG JianTao, SUN QuanMei, JIN Lin, LI Jing, WU XiaoChun & HAN Dong 2646 Programmed self-assembly of DNA origami nanoblocks into anisotropic higher-order nanopatterns FU YanMing, CHAO Jie, LIU HuaJie & FAN ChunHai 2651 p-Hydroxybenzoic acid (p-HA) modified polymeric micelles for brain-targeted docetaxel delivery ZHANG ZhiXin, WEI XiaoLi, ZHANG XiaoYu & LU WeiYue 2657 Protease-activated quantum dot probes based on fluorescence resonance energy transfer LI Xin, XUE Bing, LI Yang & GU YueQing 2663 Generalized multiparticle Mie modeling of light scattering by cells WANG Meng, CAO Min, GUO ZhiRui & GU Ning Vol. 58 No. 21 July 25, 2013 (Published three times every month) Supervised by Chinese Academy of Sciences Sponsored by Chinese Academy of Sciences and National Natural Science Foundation of China Published by Science China Press and Springer-Verlag Berlin Heidelberg Subscriptions China Science China Press, 16 Donghuangchenggen North Street, Beijing 100717, China Email: [email protected] Fax: 86-10-64016350 North and South America Springer New York, Inc., Journal Fulfillment, P.O. Box 2485, Secaucus, NJ 07096 USA Email: [email protected] Fax: 1-201-348-4505 Outside North and South America Springer Customer Service Center, Customer Service Journals, Haberstr. 7, 69126 Heidelberg, Germany Email: [email protected] Fax: 49-6221-345-4229 Printed by Beijing Artownprinting Co., Ltd., Chuangyeyuan Road, Taihu Town, Tongzhou District, Beijing 101116, China Edited by Editorial Board of Chinese Science Bulletin, 16 Donghuangchenggen North Street, Beijing 100717, China Editor-in-Chief XIA JianBai CN 11-1785/N 邮发代号: 80-214 (英) 广告经营许可证: 京东工商广字第0429号 国内每期定价: 116元 ii www.scichina.com | csb.scichina.com | www.springer.com/scp | www.springerlink.com Article SPECIAL ISSUE: Nano-Biomedical Optoelectronic Materials and Devices July 2013 Vol.58 No.21: 26342639 doi: 10.1007/s11434-012-5584-2 Sensitive monitoring of RNA transcription levels using a graphene oxide fluorescence switch ZHOU XiaoMing, LIAO YuHui & XING Da* Key Laboratory of Laser Life Science & Institute of Laser Life Science (Ministry of Education), College of Biophotonics, South China Normal University, Guangzhou 510631, China Received July 18, 2012; accepted October 26, 2012; published online December 26, 2012 Catalytic transfer of genetic information from DNA to RNA is very important in life activities. The unconventional RNA tran- scription level may be related to the source of genetic diseases. At present, conventional methods for detection of RNA transcripts usually involve cumbersome preparative steps, or require sophisticated laboratory equipments. In this study, we presented a rapid, sensitive nano-detection platform for monitoring of RNA transcript levels. T7 RNA polymerase transcription reaction is employed as the example to test the feasibility