Cleaning and Decontamination Procedure for Gilson Pipettes

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Cleaning and Decontamination Procedure for Gilson Pipettes Incorporate in Standard Operating Procedures (SOPs) in Accordance with ISO 8655 Standard EN 2 Table of contents Table of con of Table ASSOCIATED DOCUMENTS Chemical Decontamination | Cleaning |4 CLEANING AND DECONTAMINATION Precautions |3 INTRODUCTION t en t s | S T AN D A | RD O RD 3 P E R A TI T N G PR ABLE 6 | 7 OCE DUR OF E

| T CONTEN 4 S introduction 3 on ti c du tro n i ES | TT

E Gilson authorized service service Gilson authorized LSON PIP TMAN® M, lassic, Classic, TMAN® R GI E FO DUR OCE E TMAN® G, PIP ION ON PR TI T TRIMAN®. NA MI A T hapter1 L, PIPE TMAN® C ECON D D INTRODUC G AN N I CLEAN ixed, PIPE Fixed, TMAN®

onnected, MICROMAN® E, and DIS onnected, ny pipettesny must be decontaminated before sending them to their local or damaged parts, contact your local Gilson authorized service center, who will either supply you with who will either supply you center, service Gilson authorized local your or damaged parts, contact nd-users should refer to their local Gilson to End-users should refer authorized service servicing center complete for and further information. F you. for the pipette parts or who will service replacements genuine Gilson user-level center. A TE TICE ARNING TMAN® M C eo, PIPE Neo, TMAN® NO W NO the cleaning and decontamination procedure. Disposable gloves must be worn at all times. at be worn must Disposable gloves procedure. the cleaning and decontamination Decontamination implies that for any person in contact with pipettes there is a risk of there with pipettes person in contact any for implies that Decontamination contamination. throughout be necessary may clothing that other protective and any Use disposable gloves Precautions PIPE This document contains decontamination procedures suitable for PIPE for suitable procedures decontamination contains document This PIPE 4 Cleaning and decontamination C lean i 3. 2. 1. CLEANIN Variable Volume S Alcohol solutionisrecommended for cleaningPIPE The pipette must becleaned,asdescribedbelow, before itisdecontaminated. Cleaning easily becleanedanddecontaminated. PIPE 6. 5. 4. 3. 2. 1. resultsoperation and of system. the and pipette, to well damage as the parts to lead procedures re-assembly. incorrect can and re-assembly and cleaning Improper trained in decontamination by out carried Internal only be personnel should cleaning CLEANIN 6. 5. 4. n g an C lint-free cotton tissue, long-stemmed or swab lightly coated in solution. alcohol the interiorWipe of the body, with tip and nut, holder connecting either asoft-cloth, S the pipette.Disassemble Allow time for to alcohol evaporate. solution to remove any residue remaining from cleaning. with outerWipe surfaces asoft-cloth lint-free or tissue lightly coated with the alcohol Refit the tip allow ejectorand the pipette to dry. soft-cloth lint-free or tissue lightly coated with the solution. alcohol all of exposedWipe the body, surfaces with tip operating and button, holder rod, a solution. the tipWipe ejectorwith asoft-cloth lint-free or tissue lightly coated in alcohol Remove the tip ejector. Allow time for to alcohol evaporate before re-assembly. Discard and replace any damaged parts. lightly coated in solution. alcohol piston with assembly either asoft-cloth, lint-free cotton tissue, long-stemmed or swab NO c. b. a. TMAN® isdesignedsothat the partsnormallyincontact withliquid contaminants can et aside the upper part in a clean, dry place. dry in aclean, part et aside the upper arefully of the wipe O all surfaces d TICE If theIf pipette dirty, is very with abrush plastic bristles soft may used. be Remove in concave buildup surfaces. Remove wear and marks. all scuff econ G E G IN t XT a T R Only thefollowing partsmay beautoclaved: tipejector, tipholder, andconnecting nut. The upperpart(body),thepiston assembly, theO-ring,andsealofpipette are notautoclavable. your localGilsonauthorized service center. All pipettes pipettes yourself; pleasecontact your localGilsonauthorized service center. P2 andP10, F1,2,5,and10modelscontain miniaturized parts.Itisbest notto disassemble these mi efer to ERNAL ERNAL na CLEANIN ti ASSOCIA on on ingle and F ingle and P MUST | S P AR AR T AN bereverified prior to useifdisassembled. Donotdisassemble priorto sendinginto TED DOCUMEN T T D S S A RD O RD G AND G ixed M P E R A -ring, seal, and stainless-steel surface area stainless-steel of and the surface seal, -ring, C onpage7, to guideofyour lookat theuser’s pipette. TI N hapter 2 hapter odels DECON G PR OCE DUR TMAN pipettes. E

T AMINA T ION

Cleaning and decontamination 5 on ti na mi a t econ d d on page 4) , an g K and n odels i lean ultichannel) ultichannel) C ixed M ixed ES | TT E -ring. ingle andingle M LSON PIP S R GI ), 1 bar relative pressure, for 20 minutes 20 minutes for pressure, 1 bar relative ), F E FO T801575), and if trained by Gilson by and trained if T801575), L DUR OCE C (252° ingle and Single F Volume Variable ON PR TI pipettes and grease the piston prior to autoclaving; autoclaving; to prior piston and the grease ® L pipettes NA MI ) or with a soft-cloth andbrushes. a soft-cloth ) or with AN A T C (refer to to (refer User’s GuideAN L User’s ( TM Variable Volume ( Volume L Variable MAN E G TM T ECON E E odels P D D VIN I mall round brushes with soft plastic bristles may be used to be used to may bristles plastic brushes with soft Small round bath. ultrasonic immersed in another holder. of the tip clean the interior The piston assembly and seals must be degreased with isopropanol or ethanol before being or ethanol before with isopropanol be degreased and seals must assembly piston The G AN N I for P for OCLA

variable volume models with a serial number a serial models starting volume with variable N from T nly TE CLEAN NO ultichannel M ultichannel lean the individual components of the lower part of the pipette using an ultrasonic anusing part ultrasonic pipette the lean components individual of the lower the of jector locks jector spacer et the pipette volume to the nominal volume before placing it in the autoclave. the in placing it nominal before the volume to volume pipette the et terilize by steam autoclaving at 121° at autoclaving steam by terilize eave the parts to dry by evaporation or wipe them with a clean soft-cloth or lint-free or lint-free a clean soft-cloth them or with wipe parts the dry evaporation to by eave S without disassembly. a bag. dry using will and without better faster pipette the When autoclaving, S llow time for alcohol to evaporate. alcohol to for time Allow Wipe outer surfaces with a soft-cloth or lint-free tissue lightly coated with the alcohol the with coated lightly tissue or lint-free a soft-cloth surfacesWipe outer with cleaning. from remaining residue any remove to solution Wipe the entire surface of the tip holders utilizing a flossing method between method a flossing them holders surface tip the utilizing of Wipe entire the an in alcohol solution. coated lightly tissue or lint-free a soft cloth with dry. to pipette Reassemble the and allow Wipe the entire body and lower housing with a soft cloth or lint-free tissue lightly lightly tissue or lint-free a soft cloth housing with body and lower Wipe entire the an in alcohol solution. coated Remove the tip ejector and ejector spacer. the tip the Remove an in alcohol solution. coated lightly tissue or lint-free a soft cloth Wipe them with E E Tip ejector tissue. pipette Reassemble the Rinse the individual components with distilled water. distilled components individual the Rinse with L nut, tip ejector, tip holder, piston assembly, seal, and O assembly, piston holder, tip ejector, nut, tip C 50° at minutes (20 bath The following components can only The be following immersed connecting a cleaning in solution: odels O

or M ● ● ● 2. 3. authorized personnel. authorized 1. It is possible to clean PIP to possible It is the only seal using piston the lubricate lightly grease, existing remove you if however, specifiedgrease PIP in BEFORE AU PIPETMAN L without autoclavable logo fully are steam–sterilization featuring versions newer contamination. from as protection as well disassembly maximum convenience for Autoclaving M 7. 6. 4. 5. 3. 1. 2. ● ● The following components can only The be following immersed a cleaning in solution: ● F 5. 3. 4. 2. steps cansteps be taken: 1. Immersion recommendednot is method general for cleaning, following the required but if 6 Cleaning and decontamination C lean i have a negative impact on function and performance ofhave the pipette. performance and anegative function on impact the pipette. B at test Perform required. patchdecontaminant the before on entirety using chemicals of F ● ● ● ● ● ● ● ● ● in the constructionused of the pipette: ofthe supplier that the decontaminant it is compatible with the steel plastics and stainless in accordance with your own Whatever procedures. you with decontaminant check use, You may to choose decontaminate your pipette chemically, solution, bleach a10% such as Chemical Decontamination 5. 4. 3. 2. 1. ● ● ● O (body) part the upper pistonThe and of assembly the pipette are not autoclavable. F completelydry before C use. F AF n ● ● ● ● ● ● ● ● ● ● ● ● ollow processes the outline same above, exchanging solution alcohol for the chemical ollowing the autoclaving cycle, leave the pipette to cool down to room temperature and or O or g an nly the following may autoclaved: be parts T Put the parts in an autoclaving in an Put the parts bag. C C holder Tip ejector Tip PP PP (Polypropylene)PP PVD P P P P P S C A AUER A (Polyamide) B O C C et the pipette aside to stabilize at room temperature. lean the parts to autoclaved, be the parts lean especially the tip holder. onnecting nut heck that the parts are dry before the pipette. re-assembling are dry thatheck the parts utoclave for 20minutes at 121° d A S T (Polybutylene Terephtalate) (Polycarbonate) /P M (Polyoxymethylene)M ther Gilsonther P (Polyphenylene S (Polyphthalamide) (Polyvinylidene F F (Polyvinylidene B econ T (Polycarbonate/ Polybutylene Terephtalate) Polybutylene (Polycarbonate/ T T OCLA t e mindful of asolvente mindful that may leave –the asolute residue can drying after a mi na VIN ti on on I P G E | S ulfide) T luoride) T AN MAN D hecking should be carried out according out carried be to should hecking your procedure. A RD O RD ® M P C odels E , 0.1 MPa. , 0.1 MPa. R A TI N G PR OCE DUR E

Associated Documents 7 s t en Docum d e t a i ssoc A ES | TT E S T LSON PIP R GI E FO DUR

T801120 T801521 T801118 T801575 T801563 T801594 T801547 T801285 T802292 T801122 OCE DOCUMEN ON PR TI hapter3 ED NA C T MI A T ECON D D and Precision ccuracy l ASSOCIA G AN N I CLEAN onnected User’s Guide onnected l User’s Guide L User’s E eo User’s Guide L User’s Guide L Neo User’s L Part Number Part ® G User’s Guide L User’s G ® ® M User’s Guide L User’s M TMAN TMAN® TMAN® M C lassic User’s Guide TMAN® Classic TMAN® User’s Guide TMAN® Fixed TMAN® l l L Guide User’s TRIMAN®