Advance Publication The Journal of Veterinary Medical Science Accepted Date: 1 July 2019 J-STAGE Advance Published Date: 23 July 2019 ©2019 The Japanese Society of Veterinary Science Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. 1 Wildlife Science 2 Full paper 3 An immunohistochemical study on testicular steroidogenesis in the Sunda 4 porcupine (Hystrix javanica) 5 6 Anni NURLIANI1,2,3), Motoki SASAKI1,2)*, Teguh BUDIPITOJO4), Toshio 7 TSUBOTA5), Masatsugu SUZUKI2,6) and Nobuo KITAMURA1,2) 8 9 1)Department of Veterinary Medicine, Obihiro University of Agriculture and 10 Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan 11 2)United Graduate School of Veterinary Sciences, Gifu University, Gifu 12 501-1193, Japan 13 3)Department of Biology, Faculty of Mathematics and Natural Sciences, 14 Lambung Mangkurat University, South Kalimantan 70714, Indonesia 15 4)Department of Anatomy, Faculty of Veterinary Medicine, Gadjah Mada 16 University, Yogyakarta 55281, Indonesia 17 5)Laboratory of Wildlife Biology and Medicine, Department of Environmental 18 Veterinary Science, Graduate School of Veterinary Medicine, Hokkaido 19 University, Hokkaido 060-0818, Japan 20 6)Laboratory of Zoo and Wildlife Medicine, Faculty of Applied Biological 21 Science, Gifu University, Gifu 501-1193, Japan 22 23 24 25 *Correspondence to: SASAKI, M., Department of Veterinary Medicine, 26 Obihiro University of Agriculture and Veterinary Medicine, Obihiro, 27 Hokkaido 080-8555, Japan. e-mail:
[email protected] 28 29 30 Running head: STEROIDOGENESIS IN PORCUPINE TESTES 31 32 1 33 ABSTRACT 34 In the testes of the Sunda porcupine (Hystrix javanica), the expression of the 35 steroidogenic acute regulatory protein (StAR) and steroidogenic enzymes, 36 such as cytochrome P450 side chain cleavage (P450scc), 3β-hydroxysteroid 37 dehydrogenase (3β-HSD), cytochrome P450 17α-hydroxylase (P450c17) and 38 cytochrome P450 aromatase (P450arom), was immunohistochemically 39 examined to clarify the location of steroidogenesis.