Product datasheet Anti-MED9 Antibody Catalog Number: A12781

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-MED9 Antibody Name MED9 Source Rabbit IgG Species Reactivity human,mouse,rat Tested Application WB,IHC-P,Direct ELISA,ICC/IF,FCM Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E. coli-derived human MED9 recombinant (Position: A55-E146). Purification Immunogen affinity purified. Observed MW 16KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry in paraffin section: 1:50-400 Direct ELISA： 1:100-1000 Immunocytochemistry/Immunofluorescence (ICC/IF): 1:50-400 Flow cytometry (FCM): 1-3μg/1x106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information MED9 is a member of the mediator complex of that promote activation of RNA polymerase II through direct interactions with transcription factors. This MED9gene is located within the Smith-Magenis syndrome region on 17.

Reference

Anti-MED9 Antibody被引用在0文献中。

暂无引用

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-MED9 Antibody Catalog Number: A12781

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2. IHC analysis of analysis of MED9 using MED9 using anti-MED9 anti-MED9 antibody antibody (A12781).MED9 (A12781). Lane 1: rat was detected in paraffin- brain tissue lysates, Lane embedded section of 2: rat heart tissue human colon cancer lysates,Lane 3: rat kidney tissue. anti-MED9 tissue lysates,Lane 4: Antibody (A12781) . mouse brain tissue Biotinylated goat anti- lysates,Lane 5: mouse rabbit IgG was used as heart tissue lysates,Lane secondary antibody . The 6: mouse kidney tissue tissue section was lysates. anti-MED9 developed using Strepavi antigen affinity purified din-Biotin-Complex polyclonal antibody (SABC)(Catalog # (Catalog # SA1022) with DAB as the A12781)probed with a chromogen. goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for MED9 at approximately 19KD. The expected band size for MED9 is at 16KD.

Figure 3. IHC analysis of Figure 4. IHC analysis of MED9 using anti-MED9 MED9 using anti-MED9 antibody (A12781).MED9 antibody (A12781).MED9 was detected in paraffin- was detected in paraffin- embedded section of embedded section of human lung cancer mouse small intestine tissue. anti-MED9 tissue. anti-MED9 Antibody (A12781) . Antibody (A12781) . Biotinylated goat anti- Biotinylated goat anti- rabbit IgG was used as rabbit IgG was used as secondary antibody . The secondary antibody . The tissue section was tissue section was developed using Strepavi developed using Strepavi din-Biotin-Complex din-Biotin-Complex (SABC)(Catalog # (SABC)(Catalog # SA1022) with DAB as the SA1022) with DAB as the chromogen. chromogen.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Product datasheet Anti-MED9 Antibody Catalog Number: A12781

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 3

Powered by TCPDF (www.tcpdf.org)