UVRAG Antibody (Center) Purified Rabbit Polyclonal Antibody (Pab) Catalog # Ap1850c

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UVRAG Antibody (Center) Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP1850c

Specification

UVRAG Antibody (Center) - Product Information

Application WB, IHC-P,E Primary Accession Q9P2Y5 Reactivity Human, Mouse Host Rabbit Clonality Polyclonal Isotype Rabbit Ig Calculated MW 78151 Antigen Region 366-398

UVRAG Antibody (Center) - Additional Information

Gene ID 7405 Western blot analysis of anti-hUVRAG (Center) Pab (RB10847) in mouse heart Other Names tissue lysates. hUVRAG (Center)(arrow) was UV radiation resistance-associated gene protein, p63, UVRAG detected using the purified Pab.

Target/Specificity This UVRAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-398 amino acids from the Central region of human UVRAG.

Dilution WB~~1:1000 IHC-P~~1:10~50

Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This Formalin-fixed and paraffin-embedded antibody is prepared by Saturated human hepatocarcinoma tissue reacted with Ammonium Sulfate (SAS) precipitation hUVRAG (Center) (Cat.#AP1850c), which was followed by dialysis against PBS. peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data Storage demonstrates the use of this antibody for Maintain refrigerated at 2-8°C for up to 2 immunohistochemistry; clinical relevance has weeks. For long term storage store at -20°C not been evaluated. in small aliquots to prevent freeze-thaw cycles. UVRAG Antibody (Center) - Background Precautions UVRAG Antibody (Center) is for research UVRAG complements the ultraviolet sensitivity use only and not for use in diagnostic or of xeroderma pigmentosum group C cells and

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therapeutic procedures. encodes a protein with a C2 domain. The protein activates the Beclin1-PI(3)KC3 complex, promoting autophagy and UVRAG Antibody (Center) - Protein Information suppressing the proliferation and tumorigenicity of human colon cancer cells. Chromosomal aberrations involving this gene Name UVRAG are associated with left-right axis malformation and mutations in this gene have been Function Versatile protein that is involved in associated with colon cancer. regulation of different cellular pathways implicated in membrane trafficking. UVRAG Antibody (Center) - References Involved in regulation of the COPI-dependent retrograde transport from Liang,C., et al. Nat. Cell Biol. 8 (7), 688-699 Golgi and the endoplasmic reticulum by (2006) associating with the NRZ complex; the Ionov,Y., et al. Oncogene 23 (3), 639-645 function is dependent on its binding to (2004) phosphatidylinositol 3- phosphate Goi,T., et al. Surg. Today 33 (9), 702-706 (PtdIns(3)P) (PubMed:16799551, (2000) PubMed:18552835, Bekri,S., et al. Cytogenet. Cell Genet. 79 (1-2), PubMed:20643123, PubMed:24056303, PubMed:28306502). During autophagy acts as regulatory subunit of the alternative PI3K complex II (PI3KC3- C2) that mediates formation of phosphatidylinositol 3-phosphate and is believed to be involved in maturation of autophagosomes and endocytosis. Activates lipid kinase activity of PIK3C3 (PubMed:16799551, PubMed:20643123, PubMed:24056303, PubMed:28306502). Involved in the regulation of degradative endocytic trafficking and cytokinesis, and in regulation of ATG9A transport from the Golgi to the autophagosome; the functions seems to implicate its association with PI3KC3-C2 (PubMed:16799551, PubMed:20643123, PubMed:24056303). Involved in maturation of autophagosomes and degradative endocytic trafficking independently of BECN1 but depending on its association with a class C Vps complex (possibly the HOPS complex); the association is also proposed to promote autophagosome recruitment and activation of Rab7 and endosome-endosome fusion events (PubMed:18552835, PubMed:28306502). Enhances class C Vps complex (possibly HOPS complex) association with a SNARE complex and promotes fusogenic SNARE complex formation during late endocytic membrane fusion (PubMed:24550300). In case of negative- strand RNA virus infection is required for efficient virus entry, promotes endocytic transport of virions and is implicated in a VAMP8- specific fusogenic SNARE complex assembly (PubMed:24550300).

Cellular Location Late endosome. Lysosome. Cytoplasmic vesicle, autophagosome. Early endosome. Endoplasmic reticulum. Midbody. Chromosome, centromere. Note=Colocalizes with RAB9-positive compartments involved in retrograde transport from late endosomes to trans-Golgi network. Colocalization with early endosomes is only partial (PubMed:24056303). Recruited to autophagosome following interaction with RUBCNL/PACER (PubMed:28306502)

Tissue Location Highly expressed in brain, lung, kidney and liver.

UVRAG Antibody (Center) - Protocols

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Provided below are standard protocols that you may find useful for product applications.

• Western Blot • Blocking Peptides • Dot Blot • Immunohistochemistry • Immunofluorescence • Immunoprecipitation • Flow Cytomety • Cell Culture

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