DOCSLIB.ORG

A Microfluidic Coulter Counting Device for Metal Wear

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
A Microfluidic Coulter Counting Device for Metal Wear A MICROFLUIDIC COULTER COUNTING DEVICE FOR METAL WEAR DETECTION IN LUBRICATION OIL A Thesis Presented to The Graduate Faculty of The University of Akron In Partial Fulfillment of the Requirements for the Degree Master of Science Srinidhi Veeravalli Murali December, 2008 A MICROFLUIDIC COULTER COUNTING DEVICE FOR METAL WEAR DETECTION IN LUBRICATION OIL Srinidhi Veeravalli Murali Thesis Approved: Accepted: _________________________ _________________________ Advisor Department Chair Dr. Jiang John Zhe Dr. Celal Batur _________________________ _________________________ Faculty Reader Dean of the College Dr. Joan Carletta Dr. George K. Haritos _________________________ _________________________ Faculty Reader Dean of the Graduate School Dr. Dane Quinn Dr. George R. Newkome _________________________ Date ii ABSTRACT Real time monitoring of lubrication oil quality has become an important issue in today’s military, transportation and manufacturing industries. Accurate condition monitoring methodologies are needed to effectively schedule maintenance downtime as well as to ensure the accomplishment of long-range military and commercial operations. The degradation of lubrication oil is typically from two sources: 1) the accumulation of metal wear particles and 2) degradation in the physical properties of the lubrication oil. During normal machine operation, small wear debris particles with sizes in the range of 1 to 10 microns are usually generated. When abnormal wear begins, larger particles in the range of 10 to 150 microns are generated. The particle population and size will increase gradually with time and this trend of growing particle population and size will increase until machine failure. Thus, continuous monitoring of wear debris in lubrication oil is critical to avoid catastrophic system failure of machines. This thesis demonstrates that the capacitance Coulter counting principle can be used to detect and count metal wear particles generated in lubrication oil. The Coulter counting principle is an established technique to count biological cells in an electrolyte solution. A Coulter counter consists of two reservoirs connected by a microchannel. When a particle is present in microchannel, it causes a change in resistance of the liquid- filled microchannel. As lubrication oil is non-conductive, the resistance changes due iii to the passage of a particle is difficult to measure. To overcome this, we monitor the change in capacitance formed between two electrodes in a microchannel. When a metal particle passes through the microchannel, a change in the capacitance can be detected owing to the difference in permittivity between the lubrication oil and the metal particle. To demonstrate the capacitive Coulter counting principle for metal particle detection in lubrication oil, a meso-sized device consisting of two parallel electrode plates immersed in SAE-5W30 motor oil was used to simulate a fluidic channel. Metal particles were dropped from the top of the channel and allowed to travel to the bottom. It was found that the passage of the metal particle did cause a capacitance pulse, monitored using a capacitive readout IC chip. It was found that the capacitance change increases as the particle size is increased. The demonstration using the meso-sized device indicates the possibility of using a microfluidic device for detection and counting metal wear particles in lubrication oil based on the Coulter counting principle. Furthermore, to validate the dynamic response of the measurement circuitry in a fluid environment for the microfluidic device using co-planar electrodes, we first test the microfluidic device for detection of Juniper scopulorum pollen and polystyrene particles in de-ionized water. Next, we present the use of a microfluidic sensor for electronic monitoring of wear debris in lubrication oil based on the capacitance measurement setup demonstrated earlier. Aluminum particles (size ranges from 15 to 25 µm in diameter) suspended in SAE-5W30 motor oil were used for device testing. When oil with aluminum particles was loaded, capacitive pulses due to passage of these particles were observed. The magnitude of the pulses is in the range of 2 to 7 femto-farads. The test results show the microfluidic device to be highly promising for use in online debris monitoring in lubrication oil. iv ACKNOWLEDGEMENTS Grateful acknowledgement is made to my Advisor, Dr. Jiang Zhe, for his constant guidance and mentoring through the project. I would like to thank my co-advisor Dr. Joan Carletta with all sincerity for her valuable suggestions in this project. My special thanks to Ashish V. Jagtiani for his help all through.Li Du, Hui Ouyang, Abhay Vasudhev and Xinggao Xia have been of great support in this project. I would also like to extend my heartfelt thanks to my fiancé Anup Viswanathan and my family for their constant support and prayers all through my study away from home. v TABLE OF CONTENTS Page LIST OF TABLES............................................................................................................. ix LIST OF FIGURES .............................................................................................................x CHAPTER I. INTRODUCTION............................................................................................................1 1.1 In situ monitoring of lubrication oil quality.................................................................1 1.2 Coulter counting principle ...........................................................................................3 1.3 Microfluidic sensor technology ...................................................................................4 1.4 Research objectives......................................................................................................4 II. BACKGROUND WORK ...............................................................................................7 2.1 Rotary machine maintenance techniques.....................................................................7 2.1.1 Condition monitoring..............................................................................................8 2.1.2 Wear debris monitoring ........................................................................................12 2.1.2.1. Spectrographic analysis ..................................................................................12 2.1.2.2. Ferrography.....................................................................................................13 2.1.2.3. Acoustic and ultrasonic sensors......................................................................14 2.1.2.4. Chip detectors / magnetic plugs......................................................................15 2.1.2.5. Inductive sensors.............................................................................................16 2.1.2.6. Particle counting .............................................................................................17 vi 2.1.2.7. Mesh / filter blockage .....................................................................................18 2.1.2.8. Gravimetric analysis .......................................................................................19 2.1.2.9. Coulter counters..............................................................................................19 2.1.3 Visual inspection monitoring................................................................................20 2.1.4. Vibration monitoring ...........................................................................................20 2.1.5. Performance monitoring ......................................................................................21 2.2 Summary....................................................................................................................22 III. CHARACTERIZATION OF THE MEASUREMENT IC MS3110...........................24 3.1 Working principle of MS3110 IC .............................................................................25 3.2 Static characterization of MS3110.............................................................................27 3.3 Dynamic characterization of MS3110 .......................................................................33 3.3.1 Response time of MS3110....................................................................................33 3.4 Summary....................................................................................................................40 IV. MESO-SCALE SENSOR WITH PARALLEL PLATE ELECTRODES...................41 4.1 Meso-sized device and experimental setup................................................................42 4.2 Finite element model..................................................................................................44 4.3 Results and discussion ...............................................................................................46 4.3.1 Instrumentation validation ....................................................................................46 4.3.2 Detection of conducting particles .........................................................................47 4.3.3 Comparison of experimental and simulation results for pulse height of particles..................................................................................47 4.3.4 Comparison of experimental and theoretical analysis for pulse width of particles ...................................................................................50 vii 4.4 Summary....................................................................................................................54
Load more

Recommended publications
  • High-Bandwidth Radio Frequency Coulter Counter D
    APPLIED PHYSICS LETTERS 87, 184106 ͑2005͒ High-bandwidth radio frequency Coulter counter D. K. Wood Department of Physics and Institute for Collaborative Biotechnologies, University of California at Santa Barbara, Santa Barbara, California 93106 S.-H. Oh and S.-H. Lee California Nanosystems Institute, University of California at Santa Barbara, Santa Barbara, California 93106 H. T. Soh Department of Mechanical Engineering and Institute for Collaborative Biotechnologies, University of California at Santa Barbara, Santa Barbara, California 93106 ͒ A. N. Clelanda Department of Physics, University of California at Santa Barbara, Santa Barbara, California 93106 ͑Received 15 April 2005; accepted 10 September 2005; published online 27 October 2005͒ We demonstrate a method for high-bandwidth, high-sensitivity particle sensing and cell counting in a microfluidic system. Our approach employs a tuned radiofrequency probe, which forms the radiofrequency analog of a Coulter counter. By measuring the reflected rf power, this approach provides an unprecedented detection rate, with a theoretical bandwidth in excess of 10 MHz. Particle detection was performed in a continuous flow mode in a microfluidic channel, using 15 ␮m diameter polystyrene beads suspended in a sucrose-saline solution. We demonstrate 30 kHz counting rates and show high-resolution bead time-of-flight data, comprising the fastest electronic particle detection on-chip to date. © 2005 American Institute of Physics. ͓DOI: 10.1063/1.2125111͔ High throughput cell counting and analysis is an impor- are implemented using optically patterned, thermally evapo- tant biotechnological capability. Since the invention of the rated gold/titanium films ͑500 nm/10 nm thick͒ on glass Coulter counter,1 electronic means to count and analyze bio- chips.
    [Show full text]
  • Microfluidic Multiple Cross-Correlated Coulter Counter for Improved
    Microfluidic multiple cross-correlated Coulter counter for improved particle size analysis Wenchang Zhanga,1, Yuan Hu a,1, Gihoon Choib, Shengfa Lianga, Ming Liua*, and Weihua Guanb, c* a Key Lab of Microelectronic Devices & Integrated Technology, Institute of Microelectronics, Chinese Academy of Sciences, Beijing 100029, China b Department of Electrical Engineering, Pennsylvania State University, University Park 16802, USA c Department of Biomedical Engineering, Pennsylvania State University, University Park 16802, USA *Corresponding Authors: [email protected] (M. Liu), [email protected] (W. Guan) 1 The authors contributed equally. Declarations of interest: none 1 Abstract Coulter counters (a.k.a. resistive pulse sensors) were widely used to measure the size of biological cells and colloidal particles. One of the important parameters of Coulter counters is its size discriminative capability. This work reports a multiple pore-based microfluidic Coulter counter for improved size differentiation in a mixed sample. When a single particle translocated across an array of sensing pores, multiple time-related resistive pulse signals were generated. Due to the time correlation of these resistive pulse signals, we found a multiple cross-correlation analysis (MCCA) could enhance the sizing signal- to-noise (SNR) ratio by a factor of n1/2, where n is the pore numbers in series. This proof- of-concept is experimentally validated with polystyrene beads as well as human red blood cells. We anticipate this method would be highly beneficial for applications where improved size differentiation is required. Keywords: Coulter counter, particle sizing, resistive pulse sensors, multiple cross- correlation analysis 2 1 Introduction Coulter counters, also known as the resistive pulse sensors, are well-developed devices to measure the size and concentration of biological cells and colloidal particles suspended in a buffer solution[1-5].
    [Show full text]
  • Microfluidic and Nanofluidic Resistive Pulse Sensing
    micromachines Review Microfluidic and Nanofluidic Resistive Pulse Sensing: A Review Yongxin Song 1, Junyan Zhang 1 and Dongqing Li 2,* 1 Department of Marine Engineering, Dalian Maritime University, Dalian 116026, China; [email protected] (Y.S.); [email protected] (J.Z.) 2 Department of Mechanical and Mechatronics Engineering, University of Waterloo, Waterloo, ON N2L 3G1, Canada * Correspondence: [email protected] or [email protected]; Tel.: +1-519-888-4567 (ext. 38682) Received: 17 April 2017; Accepted: 21 June 2017; Published: 25 June 2017 Abstract: The resistive pulse sensing (RPS) method based on the Coulter principle is a powerful method for particle counting and sizing in electrolyte solutions. With the advancement of micro- and nano-fabrication technologies, microfluidic and nanofluidic resistive pulse sensing technologies and devices have been developed. Due to the unique advantages of microfluidics and nanofluidics, RPS sensors are enabled with more functions with greatly improved sensitivity and throughput and thus have wide applications in fields of biomedical research, clinical diagnosis, and so on. Firstly, this paper reviews some basic theories of particle sizing and counting. Emphasis is then given to the latest development of microfuidic and nanofluidic RPS technologies within the last 6 years, ranging from some new phenomena, methods of improving the sensitivity and throughput, and their applications, to some popular nanopore or nanochannel fabrication techniques. The future research directions and challenges on microfluidic and nanofluidic RPS are also outlined. Keywords: resistive pulse sensing; particle sizing and counting; microfluidics and nanofluidics; review 1. Introduction Accurately determining the size and number of particles and cells in electrolyte solutions is an important task in many fields, such as biomedical research [1–6], clinical diagnosis [7–12], and environmental monitoring.
    [Show full text]
  • Coulter Principle Short Course
    Coulter Principle Short Course DS-18639A Chapter 1 A. In a system, there exist a high number of particles. Basic Concepts in Particle Each individual particle may have different physical Characterization or chemical properties if the material is not homogeneous. The ensemble behavior is usually 1. Particles what is macroscopically observable. The macroscopic properties are derived from contributions of What is a particle? According to Webster’s Dictionary, individual particles. If the relevant property is the a particle is “a minute quantity or fragment” or “a relatively same for all particles in the system, the system small or the smallest discrete portion or amount of is deemed “monodisperse.” If all or some of the something.” Because the word “small” is relative to particles in the system have differing values for “something,” a particle can be as small as a quark or the property of interest, the system is referred to as large as the sun. In the vast universe, the sun is just as “polydisperse.” Another term, “pausidisperse” a small particle! Thus, the range of sciences and is occasionally used to describe systems with technologies for studying particles can be as broad as a small number of distinct groups. All particles we can imagine, from astrophysics to nuclear physics. within a given group have the same value for Therefore, we have to defi ne the type of particles in the concerned property. which we are interested. B. The specifi c surface area (surface area per unit “Fine Particles” is a term normally reserved for particles mass) of small particles is so high that it leads ranging from a few nanometers to a few millimeters to many significant and unique interfacial in diameter.
    [Show full text]
  • Simulation Model of a Microfluidic Point of Care Biosensor for Electrical Enumeration of Blood Cells
    SIMULATION MODEL OF A MICROFLUIDIC POINT OF CARE BIOSENSOR FOR ELECTRICAL ENUMERATION OF BLOOD CELLS BY AARON JANKELOW THESIS Submitted in partial fulfillment of the requirements for the degree of Master of Science in Bioengineering in the Graduate College of the University of Illinois at Urbana-Champaign, 2018 Urbana, Illinois Adviser: Professor Rashid Bashir Abstract Point of care microfluidic devices provide many opportunities for improving the diagnosis of a number of illnesses. They can provide a speedy, quantitative assay in the form of an easy to use portable platform. By using Finite Element Analysis software to model and simulate these microfluidic devices, we can further optimize and improve on the design of such devices. In this work we will use such software in order to model an electrical counting chamber that would be implemented in such a device. This chamber utilizes the coulter counting principle to measure the change in impedance caused when a bead or a cell passes over a series of electrodes. By utilizing the signals to count the number of cells coming into and out of a capture chamber that targets a specific antigen, we can obtain a quantitative measure of how many cells or beads were expressing the target antigen and use this for a diagnosis. First the simulation was tuned to be able to produce the characteristic bipolar pulse when a cell passed over the electrodes. Then by varying elements such as bead size, input voltage, bead composition and electrode placement and recording the results we can use this model to help further refine and optimize this device by giving us a quantitative model that will allow us to better understand how changing such variables will alter the signal received from the device and thus allow us a better understanding of the best way to get a clearer signal.
    [Show full text]
  • Flow Cytometry/Coulter Counter
    Counting Cells and Microscopic Particles: Introduction to Flow Cytometry, EpiFluorescence Microscopy, and Coulter Counters Karen Selph SOEST Flow Cytometry Facility Department of Oceanography University of Hawaii [email protected] www.soest.hawaii.edu/sfcf The Microbial World Particles to chemists, small critters to biologists… Size range (excluding viruses): ~0.4 µm to a few 100 µm’s in diameter Includes all bacteria, phytoplankton, & most primary planktonic consumers, as well as a range of abiotic particles (clay to fine sand) Ubiquitous, highly diverse functionally & taxonomically, variable activities Responsible for most of the transformations of organic matter in the ocean, as well as much of the gas transfers (O2, CO2, etc.) How do we study them? Microscopic, so need methods that will resolve small particles. Today, I’ll introduce you to 3 instruments that can resolve such small particles and give us useful information about them: – Epifluorescence Microscope – Flow Cytometer – Coulter Counter Why use an epifluorescence microscope? Quantitative detection and enumeration of microbes, including viruses Ability to concentrate particles to see rarer populations Estimate microbial biomass, using biovolume estimates. Separate classes, e.g., autotroph from heterotroph, prokaryote from eukaryote, even by species for some organisms Why use a flow cytometer? Rapid counting of microbes (minutes) Enumerate several populations in one sample, if their scatter or fluorescence signatures are distinct Enumerate dimmer cells or cells
    [Show full text]
  • Scepter™ 2.0 Cell Counter Precise, Handheld Cell Counting
    Scepter™ 2.0 Cell Counter Precise, handheld cell counting The life science business of Merck operates as MilliporeSigma in the U.S. and Canada. Scepter™ 2.0 Cell Counter Precise, handheld cell counting The Scepter™ 2.0 cell counter is your portable device option. While other automated counters consume bench space and rely on object recognition software, manual focusing and clumsy loading chambers, the Scepter™ cell counter provides true automation without the error that accompanies vision-based systems. With its microfabricated, precision-engineered sensor, the Scepter™ cell counter does all the work and delivers accurate and reliable cell counts in less than 30 seconds. Scepter™ 2.0 cell counters mark the next generation in Scepter™ technology, highlighted by: Compatibility with More Cell Types The Scepter™ cell counter is the only one on the market to accurately count particles as small as 3 μm in diameter. Increased Cell Concentration Range The new 40 μm sensor can count samples with concentrations as high as 1,500,000 cells/mL. Powerful Software for Complex, Effortless Cell Analysis • Compare sample sets side by side using histogram overlay and multiparametric data table • Create and save gating templates • Generate reports, graphs and tables Are you an existing Scepter™ device user interested in upgrading to the Scepter™ 2.0 cell counter? It’s easy. Visit MerckMillipore.com/scepterupgrade to upgrade your Scepter™ device today! 2 3 Scepter™ 2.0 │ Precise, handheld cell counting Measured Measured The power of precision Cell Type size (μm) 40 μm sensor 60 μm sensor Cell Type size (μm) 40 μm sensor 60 μm sensor Trust Scepter™ devices with your most valuable samples to get reproducible and reliable 2102 Ep 15-19 • Meg-01 16-17 • counts.
    [Show full text]
  • Microfluidic Particle Tracking Technique Towards White Blood Cell Subtype Counting and Serum Protein Quantification
    MICROFLUIDIC PARTICLE TRACKING TECHNIQUE TOWARDS WHITE BLOOD CELL SUBTYPE COUNTING AND SERUM PROTEIN QUANTIFICATION BY TANMAY GHONGE THESIS Submitted in partial fulfillment of the requirements for the degree of Master of Science in Bioengineering in the Graduate College of the University of Illinois at Urbana-Champaign, 2016 Urbana, Illinois Adviser: Professor Rashid Bashir Abstract Microfluidic technologies have gained wide acceptance in the past decade as diagnostics tools in clinical setting world-wide. This is primarily due to the fact that microfluidic technologies enable rapid, quantitative assays from small amount of physiological sample in an easy-to-use, portable platform. In this work, we will describe a microfluidic technique that can be built upon to count white blood cell subtypes or serum protein from a drop of blood. Traditionally, researchers have counted white blood cell subtypes by capturing them. However, an elegant and more accurate way to do the same is by exploiting the transitory interactions between the antigen on the surface of the cell and a cognate antibody. Cells expressing the antigen of interest will take longer to traverse a microchannel which has been coated with a cognate antibody compared to the cells which don’t express that antigen. To our knowledge, no microfluidic assay exists which can rapidly count cells using this principle. Towards this end, we have developed a repeatable experimental technique to control the transit time and the order of particles in a microchannel. To least affect the uniformity of transit time, we have also optimized the geometry of pillars in the microchannel on which antibodies are functionalized.
    [Show full text]
  • Multisizer 4E Coulter Counter
    MULTISIZER 4E COULTER COUNTER THE UTMOST PRECISION IN PARTICLE SIZING AND COUNTING AUTOMATED MULTI-PARAMETRIC ANALYSIS ACCURATE CONSISTENT THE COULTER PRINCIPLE TYPE APPROVAL CERTIFICATE US.C.27.001.A №51632 RECOMMENDED IN THE XIII EDITION OF THE RUSSIAN PHARMACOPEIA Multisizer 4e COULTER COUNTER 1953 Patented Coulter Principle 1956 The First Coulter Counter - “Model A” 1999 Patent on digital signal processing technology 2014 Release of Multisizer 4e WE CREATED THE COULTER METHOD AND CONTINUE ITS DEVELOPMENT Since the invention of the microscope, Today, the Coulter method is the standard laboratory workers have spent hours for blood tests and is used in 98% of blood estimating cell counts. The results depend to analyzers. a large degree on the user, and the process The electrical sensing zone method (Coulter is excruciatingly slow. method) is recommended in the XIII edition In 1953, the brothers Joseph and Wallace of the Russian Pharmacopeia to monitor Coulter developed a conductometric invisible particulate matter in parenteral method for counting cells, also known as solutions (OFS 1.4.2.0006.15) and to the Electrical Sensing Zone method. The determine concentration of microbial cells method is based on analysis of voltage pulses (OFS 1.7.2.0008.15). generated when suspended particles pass The method is also widely used in various through a microscopic opening (aperture), industries for quality control of starting under an electrical current. The amplitude materials and finished products. The Coulter of this pulse is proportional to the volume principle has been used as the basis of of the particle. This made it possible for the guideline documents ASTM and ISO 13319 first time to determine the size and number (Determination of particle size distributions).
    [Show full text]
  • Multisizer™ 3 Coulter Counter
    One of the highest resolutions for particle sizing and counting. Blood Banking Multisizer 3 COULTER COUNTER Capillary Electrophoresis Cell Analysis Centrifugation Genomics Lab Automation Lab Tools Particle Characterization BR-10052B The most versatile and accurate particle sizing and counting analyzer available today. Using the Coulter Principle, also known as ESZ (Electrical Sensing Zone), the Multisizer 3 COULTER COUNTER provides number, volume, mass and surface area size distributions in one measure - ment, with an overall sizing range of 0.4 µm to 1,200 µm. Its response is unaffected by particle color, shape, composition or refractive index. The Coulter Principle is the absolute leading technology in high resolution and accuracy and it is further enhanced in the Multisizer 3 by using a Digital Pulse Processor (DPP). You will get the ultra-high resolution, multiple channel analysis and accuracy not provided by any other technology. It all makes the Multisizer 3 indispensable for any industrial or life science research project involving sizing and/or counting. Equally a powerful tool for quality control, it provides the analyst with a system which is easy to use, yet so technologically advanced that it is able to solve most particle sizing problems. 1 The Coulter Principle. The method was developed by Wallace Coulter to count blood cells quickly and accurately. Its acceptance in the field of hematology is evident in that presently over 90% of automated cell counters incorporate the Coulter Principle. In the past fifty years, the method has also been utilized to characterize all kind of cells from bacteria to fat cells and thousands of different industrial particulate materials as well.
    [Show full text]
  • The Coulter Principle: Foundation of an Industry
    Special Feature The Coulter Principle: Foundation of an Industry Marshall Don. Graham Beckman Coulter, Inc. THE FIFTIETH ANNIVERSARY OF THE COULTER hopes of patenting his method for automating the Keywords: PRINCIPLE erythrocyte count.1 For each specimen, a technician Coulter Principle, spent some 30 tedious minutes at a microscope hematological Introduced in the mid-1950s, the Coulter Principle counting the red cells in a standard chamber, the instrumentation, became the foundation of an industry responding to result being only rarely repeatable. Automating such automated the need for automated cell-counting instruments. counts had tantalized Wallace (Fig. 1) since he had blood-cell counts, The industry developed in three acts, as Wallace H. read Moldavan’s 1934 note proposing photoelectric particle analysis, Coulter and his brother Joseph R. Coulter, Jr., counting of cells in a suspension as it flowed through cytometry developed the simple idea of passing cells through a capillary tube mounted on a microscope.2 Over a a sensing aperture. In Act I, Wallace’s desire to decade had elapsed before Wallace discovered automate the routine erythrocyte count led to a a better method, and a second decade was to pass simple idea, the definition of the Coulter Prin- before he was prepared to announce it. ciple, its patenting, its acceptance by the National Institutes of Health, and its description at a national conference. In Act II, the Coulter brothers ad- ACT I dressed the practicalities of a commercial instru- ment and of a business organization to support its After gathering technical experience elsewhere, manufacture and sale. In Act III, a broad research Wallace returned to Chicago in 1946 and was soon effort developed regarding volumetric errors origi- joined in his quest by his brother, Joseph R.
    [Show full text]
  • Determination of Mammalian Cell Counts, Cell Size and Cell Health Using the Moxi Z Mini Automated Cell Counter
    Journal of Visualized Experiments www.jove.com Video Article Determination of Mammalian Cell Counts, Cell Size and Cell Health Using the Moxi Z Mini Automated Cell Counter Gregory M. Dittami1, Manju Sethi1, Richard D. Rabbitt2, H. Edward Ayliffe1 1 Orflo Technologies 2 University of Utah Correspondence to: Gregory M. Dittami at [email protected] URL: https://www.jove.com/video/3842 DOI: doi:10.3791/3842 Keywords: Cellular Biology, Issue 64, Molecular Biology, cell counting, coulter counting, cell culture health assessment, particle sizing, mammalian cells, Moxi Z Date Published: 6/21/2012 Citation: Dittami, G.M., Sethi, M., Rabbitt, R.D., Ayliffe, H.E. Determination of Mammalian Cell Counts, Cell Size and Cell Health Using the Moxi Z Mini Automated Cell Counter. J. Vis. Exp. (64), e3842, doi:10.3791/3842 (2012). Abstract Particle and cell counting is used for a variety of applications including routine cell culture, hematological analysis, and industrial controls1-5. A critical breakthrough in cell/particle counting technologies was the development of the Coulter technique by Wallace Coulter over 50 years ago. The technique involves the application of an electric field across a micron-sized aperture and hydrodynamically focusing single particles through the aperture. The resulting occlusion of the aperture by the particles yields a measurable change in electric impedance that can be directly and precisely correlated to cell size/volume. The recognition of the approach as the benchmark in cell/particle counting stems from the extraordinary precision and accuracy of its particle sizing and counts, particularly as compared to manual and imaging based technologies (accuracies on the order of 98% for Coulter counters versus 75-80% for manual and vision-based systems).
    [Show full text]