NEWSLETTER

Australasian Society for Immunology Incorporated PP 341403100035 ISSN 1442-8725 December 2011 Innate Immunity in the Sunshine State Matthew Sweet Institute for Molecular Bioscience, University of Queensland

I returned to Oz after a post-doc in “sunny Toll-like Receptors, with the view that basic Scotland” about 10 years ago. Before leaving research in this area will provide insights Australia my wife and I had a vague (very into infectious and infl ammatory disease vague) idea that it would be nice to end processes. Kate Schroder’s recent return from up in Melbourne when we returned, but a CJ Martin Fellowship in Jurg Tschopp’s I guess counting more than 100 days in a lab in Switzerland has also added Nod-like row of Glasgow rain was enough to send us Receptors and the infl ammasome to our straight back to Brisbane whence we came. pattern recognition receptor repertoire. There Since 2007, I’ve had my own research group are three distinct research themes within in the Institute for Molecular Bioscience the group: TLR signalling/TLR-mediated at the University of Queensland, which infl ammation (Melanie Shakespear, Divya focuses on innate immunity in the context Ramnath, Greg Kelly, Daniel Hohenhaus); of infl ammatory pathways and responses to macrophages and Gram-negative bacterial Gram-negative bacterial pathogens. pathogens (Steve Broomfi eld, Kolja Schaale, Nilesh Bokil, Juliana Ariffi n, Tam Nguyen); Dr Matthew Sweet Currently the lab is comprised of three post- and Kate Schroder’s recently established doctoral researchers, fi ve PhD students, three team working on infl ammasome-mediated research assistants and two honours students. neutrophil activation (Kate Schroder, Our primary interests relate to the signalling Kelly Masterman, Alina Zamoshnikova, 2012 Renewal pathways and target genes downstream of Enclosed cont. p4 DO NOT DISCARD – RENEW EARLY

(RECEIPT ENCLOSED FOR AUTOMATIC RENEWALS)

Contents Innate Immunity in the Sunshine State 1 Editorial 3 Student News 3 ICB Publication of the Year Award 6 Ralph Steinman, inspiring scientist lost 8 Honouring Prof Jacques Miller 10 Councillors’ News 13 Hon. Secretary’s News 16 What’s New in Flow Cytometry 18 Visiting Speaker Program 20 Travel Award Conference Reports 21 LtoR: Kaiwen Chen, Steve Broomfi eld, Juliana Ariffi n, Kolja Schaale, Nilesh Bokil, Matt Sweet, Alina Zamosh- Upcoming Conferences 23 nikova, Kate Schroder, Melanie Shakespear, Divya Ramnath, Daniel Hohenhaus, Tam Nguyen, Kelly Masterman Publications List 25 ASI Inc. Newsletter December 2011

ASI Inc. COUNCIL Non-Voting Councillors: Newsletter Editor President Past President Dr Simon Apte Dr David Tarlinton Prof Miles Davenport Ph: 61 7 3362 0380 Walter & Eliza Hall Institute of Medical Centre for Vascular Research Email: [email protected] Research University of New South Wales 1G Royal Parade, Parkville Vic 3050 Kensington NSW 2052 Journal Editor Ph: 61 3 9345 2615 Ph: 61 2 9385 2762 Dr Gabrielle Belz Email: tarlinton@wehi.edu.au Email: [email protected] Ph: 61 3 9345 2544 Fax: 61 3 9347 0852 Email: [email protected] Honorary Secretary Honorary Treasurer Dr Susanne Heinzel Dr Pablo Silveira Visiting Speakers Co-ordinator Walter & Eliza Hall Institute of Garvan Institute A/Prof J. Alejandro Lopez Medical Research 384 Victoria Street Queensland Institute of Medical Research 1G Royal Parade, Parkville Vic 3050 Darlinghurst NSW 2010 CBCRC/I, Post Offi ce Ph: 61 3 9345 2609 Ph: 61 2 9295 8429 Royal Brisbane Hospital Qld 4029 Email: [email protected] Email: [email protected] Ph: 61 7 3845 3794 Fax: 61 7 3845 3510 Email: Email: [email protected] State Councillors New South Wales Victoria & Tasmania Council Member of IUIS Dr Stuart Tangye Dr Stuart Berzins Dr Franca Ronchese Ph: 61 2 9295 8455 Ph: 61 3 8344 5706 Ph: 64 4 499 6914 ext 828 Email: [email protected] Email: [email protected] Email: [email protected]

Queensland South Australia & Northern Territory Honorary Archivist & Webmaster: Dr Ashraful Haque Dr Michele Grimbaldeston Dr Judith Greer Ph: 61 7 3362 0414 Ph: 61 8 8222 3083 Ph: 61 7 3346 6018 Email: [email protected] Email: [email protected] Email: [email protected] Western Australia Australian Capital Territory Administrative Correspondence Dr Alec Redwood Dr Stephen Daley Ms Judi Anderson Ph: 61 8 9346 2512 Ph: 61 2 6125 7605 ASI Inc. Secretariat Email: [email protected] Email: [email protected] PO Box 7108 Upper Ferntree Gully Vic 3156 New Zealand Ph: 61 3 9756 0128 Fax: 61 3 9753 6372 Dr Anne LaFlamme Email: [email protected] Ph: 64 4 463 6093 Email: anne.lafl [email protected] ASI Student Representatives FIMSA Councillor Contact for Tasmania Ms Kate Parham Dr Guna Karupiah Dr Greg Woods Ph: 61 8 8222 3727 Ph: 61 2 6125 4562 Tel: 61 3 6226 4830 Email: [email protected] Email: [email protected] Email: [email protected] Ms Wai-Yan (Kiwi) Sun Ph: 61 8 8222 3852 Email: [email protected]

Website The ASI web site (www.immunology.org.au) has been fully remodelled and updated. New services include: ¾ Downloadable forms for ASI awards, ¾ Positions vacant pages, ¾ Jobs wanted pages, ¾ Upcoming conferences listings, as well as a plethora of links to sites of immunological interest at home and abroad. If you‛d like your lab home pages linked to the site, would like to advertise a job or conference, or have a favourite immunology-related site that doesn‛t currently appear on the ASI site, please email Judy Greer at [email protected]

Email bulletin board To subscribe to the ASI bulletin board, send an email to [email protected] with the message: subscribe anz-imm.

2 ASI Inc. Newsletter December 2011

EDITORIAL I must start by thanking the outgoing their development. This research underpins The winner of the best article of the year ASI Council Members for their fantastic current thinking in basic cell biology and is usually announced in the December contribution to the Society. In particular, biomedical research. It also has major issue but the standard has been so high Susanne Heinzel deserves a special mention; implications for new therapeutic strategies this year that I am still trying to make the role of Honorary Secretary is one that is for cancer, autoimmune and degenerative a decision as to who will receive the easy to under-appreciate from the outside. diseases.” $200, so you will have to wait until the I have been very impressed by Susanne’s March issue to learn the outcome. capacity, endless good-humour and steady hand. On behalf of the Society, I extend a Finally, thank you to all of our contributors warm welcome to the incoming members for the year and I wish everyone a safe Dale Godfrey (Vice President), Rose Ffrench and relaxing holiday season. (Hon Secretary) and Marcel Batten (NSW Councillor). The Society could not function Simon Apte without so many capable people giving their time and energy – thank you!

We have another bumper Newsletter to fi nish off the year with highlights that include a snap-shot of Matt Sweet’s Lab; the announcement of the Immunology and Cell Biology Publication of the Year Award (pp Professor Andreas Strasser 6–7); an obituary to Nobel Laureate Ralph Steinman (pp 8–9); an article describing an event held to honour Jacques Miller and the 50th anniversary of the discovery ASI STUDENT NEWS of the role of the thymus (pp 10–11); an article describing the latest advances in fl ow Hi all, It is approaching crunch time with only a cytometry (pp 18–19); and, not least, a swag few weeks to go until the 41st meeting of of travel reports and our publication list. This will be our last instalment for the ASI the Australasian Society for Immunology newsletter as the student representatives for and organisation of the social activities, In breaking news, congratulations to Andreas the ASI Annual Meeting in Adelaide! especially the student function, is in full Strasser, the winner of the prestigious swing. We are excited to announce that Victoria Prize for 2011 – Victoria’s highest In September the Adelaide branch of the we have sold out all Student Function honour for science. Andreas was awarded ASI held the 7th Annual Immunology tickets with over 90 participants expected. the $50,000 prize in recognition of his Retreat (AIR-7), which was held at the A/Prof. Simon Barry is our quiz master groundbreaking work. picturesque Warrawong Wildlife Sanctuary. for the night and we are all looking It was a weekend full of great local science forward to a night full of fun, networking To quote from the press release announcing and an opportunity to meet fellow young and the odd drink! The weather will be his award: “By exploiting mouse genetics immunologists from Adelaide. Again, we perfect and there is some talk of a Fun Professor Strasser and his team in 1989 were would like to thank our interstate speaker Run around the River Torrens during the fi rst to show that abnormalities in the Dr Jose Villadangos and local speaker Dr the meeting. We hope you will enjoy control of apoptosis can cause autoimmune Michele Grimbaldeston for sharing their the SA wine and weather. Thank you all disease or cancer and prevent tumour cells scientifi c journey with us. Apart from the for taking the time to read our updates, from responding to anti-cancer therapy. scientifi c presentations, we had a blind as I know a PhD student’s time is very In doing so, he revealed the molecular wine tasting session and wild-life guided precious! mechanism fundamental to immune tolerance night walk. For a more details, including as described by Sir Frank Macfarlane Burnet the local prize winners, please see Michele We wish the early career researchers the who was awarded the Nobel Prize in 1960 Grimbaldeston’s report in this newsletter! very best for the coming years and hope for this work. to meet some of you in December and at future ASI events! Since then he has gone on to identify several other critical components of the cell death Kate Parham and Kiwi Sun mechanism and uncovered their functions in health and disease. These include the discovery that mammalian cells have two The bottles all set up for the blind wine-tasting distinct signalling pathways that lead to apoptosis and that the BH3-only protein Bim is essential for the death of cells during 3 ASI Inc. Newsletter December 2011

Innate Immunity in the Sunshine State (cont) Dr Kate Schroder: CJ Martin Fellow Dr Kolja Schaale: Post-doctoral Researcher Kaiwen Chen). In addition, I collaborate extensively with Kate Stacey (also at the University of Queensland), who also has a major interest in infl ammasome- dependent innate immune activation.

The Institute for Molecular Bioscience is an amazing place to work. It houses 34 research groups covering a bewildering array of biological systems, approaches and technologies, and we are truly spoilt by incredible facilities and infrastructure support. It’s like being a kid in a candy shop! “Although I went to university with the idea “As a former student of the ‘Molecular Life of doing Maths (yes, what a geek!), during Science’ program at the University of Lübeck I have to say that the invitation to put my studies I quickly became fascinated with in Germany, my research interests have been an article together about myself and the Biology, and Immunology in particular. So I focused early on upon cellular and molecular lab fi lled me with some amount of dread. decided to take the road travelled in enclosed processes in the context of infl ammation Fortunately, however, I have some talent footwear instead of the road travelled in no and infection. During my graduate studies in that most useful of arts, delegation. footwear at all, and embarked on a career at the Research Center in Borstel I was Everyone has a story to tell, so I have in Immunological research. My past and dealing almost exclusively with pathogen- asked a few people from my lab to give current research interests are the interactions macrophage interactions in the context of you an overview of how they came to end between host and pathogen during the initial Mycobacterium tuberculosis infections. up working with me, and what research stages of infection and the development of Specifi cally, my PhD studies focused on the projects keep them lying awake at 3am. infl ammation. My PhD studies (2002–2005) elucidation of pathogen-induced pathways In addition to their own stories, here’s with David Hume investigated cross- driving the expression of Wnt-family a snippet on each of the fi ve people talk between innate immune signalling member proteins, description of Wnt- profi led: One has climbed Mt Everest, pathways (cytokines and toll-like receptor induced signaling pathways in macrophages one interviewed for a position with me ligands) in macrophages. My subsequent and determination of the importance of Wnt over the phone from afar whilst dressed postdoctoral position (2005–2008), focused and its pathways for infl ammatory processes. in pyjamas, one is routinely attacked by on transcriptional programs triggered by As a new member of the Sweet group, I am a vicious magpie every time he gets on macrophage differentiation and toll-like now transferring my expertise in the fi eld his bike to ride to work, one is an avid receptor ligation. Simultaneously, I was of intracellular pathogens to infections with bird-watcher who enjoys scrutinizing heavily involved in investigating various uropathogenic E. coli (UPEC), which is able the wild life with a pair of binoculars at aspects of macrophage biology as part of a to reside in intracellular reservoirs to evade hand (not sure if he routinely watches said large international consortium, FANTOM4. I host immune responses, as well as antibiotic daily magpie attack), and one excels at joined Jurg Tschopp’s group in Switzerland in treatment. Special attention is being paid to fancy dress (a Geisha and Ziggy Stardust 2009 on an NHMRC CJ Martin Fellowship, to the role of macrophages, which have so far were particular highlights). On the latter learn about another innate immune signaling been notoriously/habitually neglected in point, I have to admit that I myself have pathway, ‘infl ammasomes’. Infl ammasomes UPEC infections.” a penchant for a good fancy dress party, are molecular machines that control the and am quite proud of my track record activity of caspase-1, to regulate cytokine in this area; past efforts include Nana production and innate immune system Mouskouri, Monica Lewinsky, the Queen activation. I returned to Australia earlier this Mother and Marilyn Monroe to name year, and have established a semi-independent but a few. I live in hope that a local ASI group within Matt Sweet’s laboratory. My organizing committee will one day take team is investigating the signalling pathways on the task of co-ordinating a themed engaged by characterised infl ammasomes, fancy dress dinner at the annual ASI and their protective roles during in vivo meeting … And now, introducing a few infection. We are also characterising the members of my group: function of novel candidate infl ammasomes during infection and hereditary infl ammatory disease.”

4 ASI Inc. Newsletter December 2011

Dr Steve Broomfield: Post-doctoral Melanie Shakespear: PhD student Researcher

emergency medicine, I decided to satiate my desire for research. I undertook a “Having been fascinated with science at Master of Biotechnology program at the school and spending my time investigating “I’ve been part of the ‘Sweet-est’ group University of Queensland. As part of the various insects and assorted bugs in my at the Institute for Molecular Bioscience course I had the opportunity to get my backyard as a child, I began a Bachelor of since 2006. My interest in science was hands dirty in two lab-based projects, one Science in Biology at Murdoch University sparked during high school Biology, helped with A/Prof Kim Summers and the other in Perth, Western Australia (after initially by an encouraging teacher who made the with Dr Matthew Sweet and, once I started having a stint in the defence force). However, subject interesting and fun. I did a Bachelor generating results, I was hooked. My I quickly realized I was keenly interested of Biotechnology at the University of primary PhD project aims to understand in the application of the biological sciences Queensland followed by honours at the the differences between human and mouse to industry and medicine and subsequently Diamantina Institute before heading off macrophage antimicrobial responses to changed my degree to Biotechnology, to London where I worked as a research infection. My project involves the study completing honours in Biomedical science. assistant. I’m currently fi nishing off my PhD of a TLR-inducible zinc transporter and Following a period of time working as a thesis, which focuses on characterising the zinc in human macrophage antimicrobial research assistant in the fi eld of tumour role of histone deacetylases (HDACs) in responses to Salmonella typhimurium. immunology at Sir Charles Gairdner Hospital the TLR4 pathway in macrophages. More In addition, I also study the role of in Perth, I completed my PhD investigating specifi cally, I’m interested in identifying macrophages in the pathogenecity of the effect of toll-like receptor 7 ligands on HDACs that positively regulate TLR4- chronic urinary tract infections caused tumours in a mouse model of mesothelioma. inducible infl ammatory responses as we by uropathogenic E. coli. In addition to Currently I am working as a post-doc in the hope this will enable new approaches to Matt (my primary supervisor), Dr Kate Sweet laboratory at the Institute for Molecular anti-infl ammatory drug design (given that Stacey and Prof. Mark Schembri act as Bioscience, investigating macrophage broad-spectrum HDAC inhibitors are already co-supervisors for my PhD studies. When autophagic antimicrobial responses to used clinically as anti-cancer drugs). Life is I am not in the lab slaving away to satisfy Salmonella typhimurium, amongst others. pretty hectic right now as I juggle the fi nal the whims of my supervisor, you will fi nd This relatively new fi eld of autophagy two chapters of my PhD thesis, as well as me either playing sport, bush walking or research, which is involved in a number of my newborn daughter!” stargazing with my astronomy club. My key cellular processes and diseases, is both other guilty pleasure is video games; exciting and challenging!” Dr Nilesh Bokil (MBBS): PhD student 4-player battles on my PS3 with my fl at The joy and beauty of science was instilled in mates can get really violent.” me from a very young age. I was fascinated by the inner workings of the human body PS: If you are interested in the roles of and this led me to medical school at M.S. pattern recognition receptors in health and Ramaiah Medical College (Bangalore, disease then don’t miss TLROZ2012, a India). Though I enjoyed the clinical work, conference dedicated to exactly this – this I had a nagging desire to do research. After meeting will be held in Melbourne from successfully completing my medical training 2–4 May, 2012. Never miss the chance and working in the UK and in India, including for a free plug I say! a stint as chief resident in the department of

5 ASI Inc. Newsletter December 2011 Immunology and Cell Biology Publication of the Year Awards 2010

It is a great pleasure to announce the the education of NK cells. Little is known winners of the Immunology and Cell about the development of NK cells in Biology Publication of the Year Awards. hematopoietic and visceral organs during Recipients of the awards must be a fi rst ontogeny and whether organ-specifi c niches author on one of the following ICB alter their development. Dr Andrews clearly manuscript categories: Original Article, shows that maturation of NK cells occurs very Outstanding Observation, Theoretical early in life. In the absence of B and T cells, Article or Brief Communication. They key components of the adaptive immune must also be fi nancial members of the system, NK cells do not develop in the bone Australasian Society for Immunology marrow or spleen but rather accumulate in Inc. (ASI) by October of the year in which the liver. Thus, this work reveals that NK cell the article was published. Two awards development in the liver occurs independent have been established for outstanding of NK cell development in the bone marrow publications. The winner of the Chris and these cells are then able to seed the spleen and Bhama Parish ICB Publication of and bone marrow. the Year Award is awarded a AU$1000 scholarship provided by the Nature Dr Andrews completed his PhD at the Publishing Group and the runner-up is University of Western Australia with awarded a AU$500 scholarship provided Professor Mariapia Degli-Esposti in Dr Patrick Reading, recipient of the runner-up award by Thermo Fisher Scientifi c. 2004. During these studies he was able to for the 2010 ICB Publication of the Year demonstrate that Murine Cytomegalovirus This year, a small committee consisting (MCMV) could functionally impair dendritic Dr Reading completed his PhD at the University of the ASI President and Executive Board cells, a fi nding that resolved the issue of of Melbourne where he investigated the role Members of ASI selected the two best how MCMV induced immunosuppression. of mannose-specifi c lectins in innate host articles based on scientifi c excellence. I In addition, he investigated the critical defense against infl uenza. He then moved to am pleased to announce that Dr Daniel interactions that occur between dendritic cells the Sir William Dunn School of Pathology, Andrews was chosen by the committee and NK cells in mediating immune protection Oxford, UK, where his focus changed to as the winner of the 2010 Chris and during cytomegalovirus infection. This later understanding the elegant strategies used Bhama Parish ICB Publication of the work on NK cells led him pursue research on by poxviruses to elude immune detection. Year Award, and Dr Patrick Reading was the development of NK cells with Professor It was here he developed a keen interest in chosen as the runner-up. Mark Smyth (Peter McCallum Cancer Centre, the contributions that the innate arm of the Melbourne) as a Peter Doherty Fellow. He immune system might make to protecting The winning paper by Dr Andrews is has continued to explore his interest in NK against pathogens. In 2003, Dr Reading an Outstanding Observation entitled cells and has now expanded this work to returned to the University of Melbourne ‘A potential role for RAG-1 in NK cell understand regulation of NK cells through (Microbiology and Immunology) where he development revealed by analysis of NK Ly49 interactions with non-classical Major began to investigate the role of NK cells and cells during ontogeny’, published in the Histocompatibility Class I molecules and to other innate cell types following infl uenza January/February 2010 issue of ICB.1 understand novel roles of granzymes during and herpes virus infections. He now holds a In this study, Drs Andrews and Smyth infl ammation and cytokine release. joint appointment as a research fellow at the explore the role of adaptive elements in University of Melbourne and as an educator Dr Reading’s study entitled ‘Infl uenza viruses at the WHO Collaborating Centre for differ in ability to infect macrophages and Reference and Research on Infl uenza. This to induce a local infl ammatory response allows him to continue to pursue research following intraperitoneal injection of mice’ into the innate immune system in addition to was published in the July/August 2010 making a signifi cant contribution to infl uenza issue of ICB2. He analyzed the capacity for pandemic planning and preparedness in the different infl uenza viral strains to infect Asia/Pacifi c Region. the macrophages and induce cytokine production. Intriguingly, he found that The award winning papers of Dr Andrews and different infl uenza viral strains differed Dr Reading highlight the very high standard signifi cantly in their ability to recruit and of both the Outstanding Observations and activate other leukocytes and to induce the Original Articles published in ICB. I hope production of particular types of cytokines. these publications encourage others to submit His work suggests that the ability of infl uenza their outstanding research to the journal. strains to infect macrophages, and elicit cytokine production, may be critical factors Dr Daniel Andrews, recipient of the 2010 Chris and Bhama Parish ICB Publication of the Year regulating the extent of the infl ammatory Award response. 6 ASI Inc. Newsletter December 2011

References 1 Andrews DM, Smyth MJ. A potential role for RAG-1 in NK cell development revealed by analysis of NK cells during ontogeny. Immunol Cell Biol 2010; 88: 107-116. 2 Reading PC, Whitney PG, Pickett DL, Tate MD, Brooks AG. Infl uenza viruses differ in ability to infect macrophages and to induce a local infl ammatory response following intraperitoneal injection of mice. Immunol Cell Biol 2010; 88: 641-650.

Gabrielle T. Belz Editor-in-Chief, Immunology & Cell Biology Division of Molecular Immunology, Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3052, Australia. Email: [email protected]

This article was originally published in Immunol Cell Biol. 2011 Oct;89(7):747-8. doi: 10.1038/icb.2011.77 and is reproduced with permission.

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7 ASI Inc. Newsletter December 2011 Ralph Steinman: A Nobel Prize gained, but an inspiring scientist lost

Immunologists were delighted to learn epidermal Langerhans on October 3rd that Ralph Steinman cells were an “immature” had been awarded the 2011 Nobel form of DC that could Prize for Physiology and Medicine, a develop into “mature” prize shared with Bruce Beutler and T-cell activating DC on Jules Hoffmann. But this was almost migration into lymph immediately overshadowed by the news nodes. Steinman’s early that Ralph Steinman had fi nally lost his work on antibodies battle with pancreatic cancer, and sadly against DC surface had passed away before even knowing of molecules with the award. ASI members will remember Michel Nussenzweig, well his valuable contributions to our his first PhD student, 2010 Conference in Perth. Several of us eventually led to their involved in dendritic cell (DC) research recent collaboration on wish to make this tribute to Ralph as the enhancing vaccines by father of our research fi eld and as a valued targeting antigens to friend and advisor. Our sympathies go to DC in situ. Much of our Ralph’s wife and family, to his friends current picture of DC as and to his colleagues at the Rockefeller the antigen-collecting University. sentinels of the immune system, as the sensors Ralph Steinman was a Canadian, of pathogen invasion, as although most of his research career the most effi cient cells was at the Rockefeller University, New for processing antigen York. He began there as a post-doctoral for MHC presentation fellow and progressed to be the Henry G and as the initiators Kunkel Professor. His research on DC and regulators of T cell began in 1973 with Zanvil Cohn when responses, we owe to Ralph Steinman. Portrait of Ralph Steinman with his Dendritic Cells, by Carol Hill he identifi ed cells with the characteristic dendritic form in spleen cell preparations. All of us have strong, positive memories of Steinman was an essential step in having He considered them a special cell type, Ralph as an inspiring scientist and friend. our model of DC function established. Bill but few were convinced at that time. It Derek Hart remembers his perception and Heath appreciates the support his work took over 10 years before the special determination to distinguish DC from other received in the mid ’90s when Ralph was antigen-presenting role of DC was cells in the early days when this view was keen to identify studies that placed DC in generally recognised; the pivotal fi nding considered laughable. Andrew Lew also a key tolerogenic mode. Ralph was in a was that virtually all of the capacity to notes the remarkable power of his convictions position where his opinion had additional, stimulate T cells in mixed leucocyte at this time. Derek Hart and Gabrielle and what some considered as excessive, reactions could be attributed to the Belz both found that, despite his strong weight, since he was also editor of the numerically minor DC component of convictions, Ralph was willing to change Journal of Experimental Medicine. So it lymphoid cell suspensions. Steinman was his mind in the light of data. We have been is worth recounting a recent experience of not the only one to describe cells with told that Ralph dismissed early evidence for Ralph in editorial mode. Barbara Fazekas dendritic or veiled cell morphology, nor antigen processing, but this certainly changed and her colleagues have produced evidence the only one to investigate their functions. as he investigated the process in collaboration that Langerhans cells are always tolerogenic, However he was the one who showed the with cell biologists such as Ira Mellman. a concept that met considerable resistance greatest persistence in these early years, Ralph had an exceptional ability to absorb and certainly contradicted Steinman’s earlier who designed the clearest experiments and process information from lectures and models. Nevertheless, having read their and who established a remarkable personal discussions at scientifi c meetings, manuscript, Ralph agreed to guide it through school of research into DC biology. and integrate it all in his meeting summaries. the review process of Proceedings of the Steinman devised the early procedures Andrew Lew remembers the look of intensity National Academy of Science, and one of his for DC isolation, was the fi rst to use as Ralph listened carefully to Andrew’s fi nal actions was to approve its acceptance fl ow cytometry to characterise the DC own scientifi c story, but also how that focus for publication. by surface markers and he produced the immediately shifted to Bill Heath and Frank fi rst mAb against DC surface molecules. Carbone when they arrived! Barbara Fazekas considers that for Ralph Steinman together with Gerald Schuler the science and the people involved were and Niki Romani demonstrated that We all believed that convincing Ralph inextricably linked. Ralph’s extended

8 ASI Inc. Newsletter December 2011 scientifi c family, which included not only this went beyond DC, since both Ralph and the many students and post-docs who had Ranjeny had twins, and both loved to dance trained in his laboratory but also long- the tango. standing collaborators and colleagues, would meet every two years at the International DC For Jose Villadangos and Ranjeny Thomas, Symposia. His introduction of speakers at one of Ralph Steinman’s main contributions these meetings involved carefully researched was championing translation of basic personal background with appropriate slides. research on DC into medical application. Ken Shortman recalls chairing the opening He mentored many clinical scientists in symposium with Ralph Steinman at the translational projects in his laboratory, Kobe International DC Symposium. It was including Nina Bhardwaj, Paul Cameron and Ralph’s fi rst venture from his home base after Madhav Dhodapkar. His lectures on taking treatment for pancreatic cancer, but he was DC into medicine were inspirational, as are determined this conference organised by his his articles on this subject with the like- Japanese colleagues would start on the right minded Jacques Banchereau. One of Ralph’s Ralph Steinman at the 2010 ASI conference note. Ken received beforehand a detailed recent activities was to found and promote the (Photo: Alan Baxter) script and slides to ensure this was the case! International Society for Dendritic Cell and Ralph’s energy, enthusiasm and commitment Vaccine Science (http://www.dc-vaccine. were unabated by his illness. org/) with the goal of advancing this fi eld and medical practice. Our fi nal tribute to attracting young scientists to the discipline. Ralph will be to build on the foundation Ralph also had considerable personal charm. Ralph even applied DC immunotherapy to he provided. Ken Shortman’s wife Carol responded to this his own tumour, an intervention that may and asked if she could paint his portrait from have extended his life. Contributed by: Derek Hart, Ranjeny a photograph; her tribute to Ralph is included. Thomas, Barbara Fazekas de St. Groth, Ranjeny Thomas will always remember the Ralph Steinman has left us with a substantial Bill Heath, Frank Carbone, Jose way Ralph would ask, whenever they met at legacy: an in-depth knowledge of DC biology Villadangos, Gabrielle Belz, Andrew Lew meetings, about their “passions in common”; and new ways to apply this knowledge to and Ken Shortman.

9 ASI Inc. Newsletter December 2011 Honouring Professor Jacques Miller Robyn Slattery Head, Diabetes Research Team, Dept of Immunology, Monash University, Alfred Hospital, Melbourne

On 26th August, 2011, the Australasian laughter. Richard spoke also of the founding Society of Immunology sponsored a impact Jacques had on the establishment very special event to commemorate the of Immunology as a discipline for science 50th anniversary of the discovery of students, leading to the formation of the the role of the thymus, and to honour Department of Pathology and Immunology, the man who made the discovery now Immunology, at Monash, and on many – Professor Jacques Miller. This unique of the careers of those in the audience and celebration of creativity in science and Australia wide. He also acknowledged the art was an initiative of the Department remarkable inspiration Jaq was as a scientifi c of Immunology, School of Medicine, mentor but all the time remaining a truly at Monash University and held at their modest gentleman. Alfred Hospital Campus. The fi nal tribute to Jaq was delivered by the Dr Norman Swan, from ABC’s Radio organizer and author of this event coverage, National Health Report, was the Master A/Professor Robyn Slattery, who spoke on of Ceremonies, generously donating his behalf of Jacques’ biographer, Ms Maryna time to travel from Sydney for the event. Blankenstein-Gabert. Norman’s insight into medical research and style gave the evening gravitas and identified B-cells. There isn’t a single ‘Writing about Jacques Miller one is humour. He introduced the evening, and advance in vaccine, immunotherapy or auto- constantly reminded of Shakespeare’s lines Jacques, to the crowd of ~100 attendees immunity research that doesn’t incorporate from Twelfth Night: by commenting on Jaq’s trademark Jaq’s thinking”. Finally, with his typically shyness as he sat quietly, in the shadow positive outlook, Peter pointed out that not “.... some are born great, some achieve of his brightly lit portrait, suggesting only did Jaq deserve a Nobel Prize, but that greatness and some have greatness thrust he might come out a little later in the he is still in the running – at least for fi ve upon them”. evening to say a few words. Norman more years, since Peyton Rous, who in 1911 paid great respect to the work Jaq had discovering that cancer could be transferred This evening we celebrate the 50th done 50 years earlier and reminded all by viruses, had waited 55 years to receive the anniversary of his discovery of thymus that, without an understanding of how Nobel Prize – indicating, as Peter put it “that function but even without the greatness of the thymus functioned, and how T and the Nobel committee didn’t think much of his epic discovery he was always destined B cells collaborated to form the army chickens”. The audience clearly appreciated for a life of prominence. that is our adaptive immune system, we the sentiments Peter expressed and his fi tting would not understand how HIV ravages tribute to the contribution Jaq has made to This evening he is surrounded by eminent the body, how vaccination works or Immunology, Medicine and humankind. The friends and illustrious colleagues, here to why some people develop autoimmune person who appreciated Peter’s words most honour his achievements in the world of diseases. Norman’s deep understanding may have been Jaq himself, who smiled with immunology and to celebrate his art. But with of human health and his eloquence of gratitude from the corner of the room. a different twist of fate we could likewise communication allowed every person have assembled to mark the elegance of his in the audience to comprehend the Richard Boyd, Professor of Immunology writing or the eloquence of his lectures or magnitude of Jaq’s discovery in the at Monash, and once a long-haired, even his mastery of the Italian language. context of everyday life. Norman seventies, surfi ng bum come Immunology introduced Laureate Professor Peter student, spoke about riding the crest of His wit is marvellously illustrated by the Doherty to give the opening address and the Immunology wave in the decade that anecdote of him sitting an oral exam in tell the deeper story of what it was that followed Jaq’s momentus discoveries of Italian: after much effort to help Jacques Jaq did 50 years ago and published in the thymus which lead to the dichotomy overcome his nerves, the examiner fi nally September 1961 in The Lancet. of the origins of T cells and B cells. They set the question: were heady days for a young wild student, Having shared, in 1996 with Rolf and though Jaq is known for his love of “What would you do if you returned home to Zinkernagel, the Nobel Prize for long hair, Richard reminisced that his own fi nd your wife in bed with another man?” elucidating how T cells kill viruses, salty tangled mane may have presented as Peter was well poised to comment on the somewhat of a culture shock for the serious Anticipating an outraged Latin reaction he importance of Jaq’s discovery, pointing scientist and hard-task-master Jacques was overcome with admiration for Jacques’ out that “Not only had Jaq discovered Miller! For those in the audience who had reply: that the thymus produces T-cells that been privileged to work closely with Jaq, circulate through the blood but he also Richard’s words rang true and roused much

10 ASI Inc. Newsletter December 2011

LtoR: Prof Stephen Jane (new Head of Central Clinical School at Monash), Dr Norman Swan (MC), Peter Doherty, Richard Boyd, Robyn Slattery, Jacques Miller (Photo: Monash University)

The centrepiece of the art exhibition was Publicity surrounding the event such Jaq and Jill (Photo: Monash University) Jill’s impressionist style portrait of Professor as ABC’s Radio National broadcast Jacques Miller contemplating cells of the by Norman Swan (http://www.abc. immune system. After Jill spoke, the silent net.au/rn/healthreport/) and an article “Salterei subito a letto insieme con loro” auction was announced, and after a fl urry in The Australian two days later ‘I would immediately hop into bed with of bidding by three competing interests, the (http://www.theaustralian.com.au/ them.’ portrait was sold to Mrs Tina and Mr Sam news/health-science/nobel-prize-on- Germana, for $4100. Tina had studied Italian the-cards-for-immunologist/story- This evening, we might have just as easily with Jaq some 20 years ago and said of the e6frg8y6-1226143044716), and since been celebrating Jacques’ 80th birthday and portrait, “It will be like having our friend at the announcement of the 2011 Nobel his success as a musician ... or chess master home with us”. Prize in Physiology and Medicine (http:// ... or why not ballet critic? www.theaustralian.com.au/news/health- The exhibition, which continued over the science/a-sorry-case-of-nobel-neglect- Some are born great and some do indeed have weekend, also showcased many of Jill’s as-a-pioneering-scientist-misses-out- greatness thrust upon them but Jacques Miller impressionist landscape pieces, and a number again/story-e6frg8y6-122616683), would have achieved greatness, whatever of Jaq’s brush and ink nude sketches, the sales have all helped to raise awareness for his chosen fi eld. from which Jacques donated to the Jacques Jaq’s groundbreaking work 50 years Miller Foundation. The event raised revenue ago and the wonderful legacy Jaq and Let’s raise our glass to celebrate Jacques’ of $6,318 for the Jacques Miller Foundation. his contemporaries have created; that is gift of friendship and contribution to This ASI-sponsored event raised funds that Immunology in the world today. mankind.’ will be used to support the training and research of young immunologists at Monash At the close of the three tributes to Jacques, University. Norman Swan introduced Ms Jill Steenhuis, the artist who was commissioned to paint Jacques’ portrait, and had travelled from the South of France to donate the portrait to Monash to raise funds for the Jacques Miller Foundation. Jill spoke about the artistic process and the journey to be at-one with nature, to perceive its beauty and to create. The challenge in art – to take the risk to be truly creative – Jill likened to creativity in science, quoting Emily Dickinson: “It might be easier to fail with land in sight than gain – my blue peninsular – to perish – of delight”.

Sam and Tina (who purchased the portrait) with Jaq and Jill (Photo: Monash University)

11 ASI Inc. Newsletter December 2011

12 ASI Inc. Newsletter December 2011 ASI Councillors’ News N.S.W. News University (St Louis Missouri) who updated give very impressive plenary talks. us on his studies on Ag presentation and Locally, Mariapia Degli-Esposti, Lloyd First, congratulations to all NSW diabetogenesis. Both of these seminars D’Orsogna, Anthony Bosco and Anna immunologists who are recipients of ASI were very well received and both speakers Nowak also gave impressive invited Travel Awards to attend the upcoming annual seemed to really enjoy their brief time in seminars. ASI2011 meeting in Adelaide. Well done, Sydney. Next will see Dr Warren Leonard and no doubt well deserved. And second, (National Heart Lung and Blood Institute, Purposefully, the meeting has a laidback congrats to those immunologists fortunate NIH) arrive in town to give a seminar on a atmosphere with the intention that local enough to be recipients of NHMRC funding topic close to the heart of many Australian immunologists can get together and for 2012 – it’s a cut throat profession we immunologists: the common gamma chain catch up with old friends and colleagues have all chosen! family of cytokines. I’m sure this visit will as well as to make new contacts and be highly successful as well. collaborations. The vast majority of Now, to re-cap on recent highlights of people responding to the survey indicated activities for ASI NSW. In August, the third After three years, this marks the end of my that they would prefer this meeting to be ASI NSW/ACT Combined Retreat took tenure as ASI NSW Councillor – and I am held annually and for two days. We are place in Bowral in the Southern Highlands very pleased to be passing the baton to the in the process of determining if this is of NSW, half-way between Sydney and very capable hands of my colleague Dr feasible and how we would incorporate Canberra. This meeting was another great Marcel Batten (Garvan Institute) who I know the student and early career workshops. success, with ~90 delegates attending from will do an excellent job in this position. If others in the Perth community have a most of the major research institutes and view on this, please let me know. universities throughout the greater Sydney All the best. area and the ACT. Please see the report by Stu Tangye In other news, the ASI hosted a talk by Steve Daley for all the details. But I will Councillor Professor Marc Dalod from the Centre just reiterate Steve in thanking the invited d’Immunologie Marseille-Luminy, speakers (our President David Tarlinton; Université de la Méditerranée on October Cecile King; Chris Goodnow, Julia Ellyard W.A. News 31st. Professor Dalod was in Perth to visit and Tri Phan), our very generous sponsors the laboratory of Mariapia Degli-Esposti. (Miltenyi, the major sponsor; Jomar The last quarter has been a busy time for In December the ASI will also be hosting Bioscience; Becton Dickinson; StemCell immunology in Perth. We held the second Astrid Westendorf so keep an eye out for Technologies; Invitrogen and Australian Perth Immunology Group (PIG) meeting the fl yer announcing the time of her talk, Biosearch) and, of course, all those who from 20–21 October. The event was held “Induction and expansion of regulatory T attended and presented and made the meeting on the banks of the Swan River at the Perth cells at mucosal surfaces”. This is Astrid’s a great success. I’d also like to thank Steve Flying Squadron Yacht Club in Dalkeith. fi rst visit to Australia so I hope you all for rounding up a busload of Canberrans to The feedback from the 49 delegates and make her feel welcome. attend (whilst juggling the arrival of baby invited speakers was that the meeting was #3), and Scott Byrne for putting together a success. Stuart Tangye from the Garvan Well, that’s a wrap for WA news, I’m off the scientifi c program. Institute for Medical Research in Sydney to watch the Melbourne Cup. and Thomas Gebhardt from Microbiology One goal of mine in taking on this position and Immunology at the University of Alec Redwood was to increase exposure of immunologists Melbourne both kindly fl ew to Perth to Councillor from different sites to the research being done across the State, with the hope that this will lead to new collaborations and interactions. And hopefully that goal has been fulfi lled by the combined NSW/ACT retreat. So I would like to thank all the lab heads who have contributed to the success of this meeting by encouraging (and paying for) attendance by their lab members at this meeting.

Plenary speaker Stuart Sydney has recently hosted visits by several Tangye being presented invited speakers as part of the ASI-sponsored with hand made pens Visiting Speaker Program. These have as a thank you by local included Assoc Prof Shane Crotty from the La ASI committee member and chair of the session Jolla Institute for Allergy and Immunology Andrew Currie. (California), who presented his lab’s latest fi ndings on T follicular helper cells, and Prof Emil Unanue from Washington

13 ASI Inc. Newsletter December 2011

A.C.T. News Queensland News NSW/ACT Retreat: Since 1999, an important event of The 3rd Annual ASI NSW/ACT the Queensland ASI calendar has Combined Branch Retreat held on 25- been the Brisbane Immunology 26 August 2011 was another highly Group Annual Retreat. This year successful retreat for ACT and NSW was no exception, with the event immunologists. As in the two years being held on August 18-19 at preceding, the meeting was held in Sea World, Gold Coast. ASI idyllic surrounds at Craigieburn Resort continued its support of this event & Conference Centre, Bowral. by subsidizing registration for the 57 ASI members who attended. Special thanks go to the invited ASI was also honoured to award keynote speakers, Cecile King and the Peter Doherty Medal for best David Tarlinton, who gave excellent postgraduate presentation to Dr Ranjeny Thomas, Emil Unanue & Michael Good presentations. There were also insightful Siok-Keen Tey (Tumour Immunology and exciting talks by Chris Goodnow and Lab, QIMR), and the prize for best poster Julia Ellyard on the latest in Deep/Next to Dr Motoko Koyama (Bone Marrow S.A./N.T. News Generation sequencing, and by Tri Phan Transplantation Lab, QIMR). on Intravital/2-photon imaging. The organizing committee is now in the Also, in an effort to increase Interstate fi nal preparation stages for the 41st ASI The bulk of the Scientifi c Program, communication amongst Australia-based Annual Scientifi c Meeting to be held in kindly put together by Scott Byrne, immunologists, the ASI Queensland branch Adelaide, 11–15 December. We have an was devoted to a string of impressive funded trips for various speakers such excellent array of top international speakers presentations from students and post- as Dale Godfrey, Frank Carbone, Steve (Lisa Coussens, Richard Flavell, Michael docs. The strength and depth of the Turner (Department of Microbiology and Karin, Paul Kubes, Alberto Mantovani, Ed science made it diffi cult for the judges to Immunology, University of Melbourne) Palmer, Shigeru Saito, Joachim Schultze, allocate prizes, but the deserving winners and Jose Villadangos (WEHI) to visit and Megan Sykes, Andrew McKenzie, Prof were Lucinda Berglund, John Altin and speak at QIMR. Michal Schwarz and Prof. Raz Yirmiya). Monika Srivastava who received travel The scientifi c and social programs are now prizes to the value of $750, $500 and $250 Like many other State branches, the set and we hope that the conference will respectively to attend a scientifi c meeting Queensland ASI branch has been very keen be scientifi cally stimulating and provide of their choosing. Congratulations also to participate in the International Visiting multiple opportunities to network and hear to Dr Tyani Chan for taking out the Post- Speakers Program. Early in October, Emil about the excellent research being undertaken doc/early career fellow prize, which is a Unanue, a veritable Pillar of Immunology around the country. cash prize of $250. himself, wowed a pack seminar room at the Institute of Molecular Bioscience, UQ, 7th Adelaide Immunology Retreat (AIR-7) Our thanks go to the following companies with his studies on antigen presentation in 2011 Report for their generous sponsorship of the a murine model of Type I diabetes. Emil Once again, the Adelaide Immunology meeting: Miltenyi Biotec, Becton also enjoyed the company of eminent Retreat (AIR) was a great success. It was Dickinson, JOMAR Biosciences, immunologists Ranjeny Thomas, Michael held at Warrawong Wildlife Sanctuary on Invitrogen Life Tech, Stem Cell Good and Jon Sprent over an informal dinner. September 2 & 3. The retreat was opened Technologies and Australian Biosearch. The Queensland branch members benefi tted with a terrifi c seminar on DCs by Prof. Jose Special thanks must also go to Stuart greatly from Emil’s visit who, as a die-hard Villadangos (WEHI), our invited national Tangye who initiated this meeting in baseball fan, is no doubt ecstatic that his speaker. This was followed by presentations 2009 and established it on the calendar team, the St Louis Cardinals, won the World from PhD students, Honours students and of NSW and ACT immunologists, not Series this year! research assistants. Overall the standard least due to Tangye Trivia, his distinctive of the presentations was exceptional. brand of evening entertainment. At the time of writing, the Queensland ASI Congratulations go to the following students branch is looking forward to welcoming who received awards: Mark Bunting (AIR-7 Stephen Daley Prof Warren Leonard from the National Best PhD Presentation), Wai Yan Sun (2nd Councillor Heart, Lung and Blood Institute, NIH, USA, PhD Presentation Prize), Ervin Kara (Best on November 9, when he will be giving a Honours Presentation) and Carly Gregor seminar at QIMR. And from then, it’ll be a (2nd Honours Presentation Prize). short hop, skip and a jump to Adelaide for the ASI Annual Congress. Hope you can In addition to the science presented, we also make it. participated in some terrifi c social activities in the lovely surrounds of Warrawong Best wishes, Wildlife Sanctuary. A very popular wine Ashraful Haque tasting function was held and prizes awarded Councillor 14 ASI Inc. Newsletter December 2011 for guessing the vintage, type of grape and Victorian News become ASI members. The venue and the winery that produced the wine. Special meeting itself proved highly successful thanks to Cara Fraser and Erin Lousberg (great job by the organizing committee) who organized this fun activity. Following with plenty of folk coming from Deakin this, we were treated to a dusk walk around and CSIRO who hadn’t previously been the sanctuary and we were privileged to 2011 was a great year for immunology in to an IgV meeting, and we even had a see two platypuses in the sanctuary’s lake. Victoria. The main highlight was probably few intrepid visitors from Tasmania, This is a rare occurrence and was certainly the maintenance of federal research funding which was fantastic. There won’t be an one of the highlights mentioned by many when many had initially thought it would IgV meeting next year because the ASI of the students after the retreat. Another drop by 20% or more. Instead, federal funding Annual meeting is coming to Melbourne, memorable event was the extreme weather remained largely unchanged, thanks in no but there will be plenty of IgV sponsored we all experienced overnight. Very few of us small part to those among us to who put a events and awards, so please keep an eye slept as our tent-cabins were nestled amongst public face to science and lobbied through out for announcements. large gum trees and the stormy gale-force the ‘Discoveries need Dollars’ initiative. Well winds threatened to rip the tents off their done to all those who got involved. Although the 2012 meeting is over 12 moorings. Apparently Jose lay awake in his months away, preparations are well tent-cabin wondering if one of the very large We had another series of excellent speakers underway and it looks like being a great gum trees was going to drop on his head. And come to Victoria through the ASI Visiting success with over 800 people expected to I, on the other hand, worried all night about Speakers Program. I don’t wish to single out attend. Keep your eye on the ASI website insurance if anything untoward did occur. any one in particular, but the most memorable for details (http://www.immunology.org. Fortunately, we all emerged bleary-eyed the for me was the talk by Professor Warren au/). Speaking of the website, we expect next morning and very much relieved that Leonard at the GTAC centre in Parkville. to be updating it in the New Year and no incidences were reported. Needless to Prof. Leonard talked about the role of may yet dip our toes into social media say, this is one retreat many of us will never common gamma chain family of cytokines such as Facebook and Twitter as IgV has forget for many reasons! in immune regulation, and the interest in already done. I’ll let you know if and his seminar saw the lecture theatre packed when that happens. Finally, I would like to thank the AIR-7 to overfl owing. All went smoothly in his organizing committee members Cara Fraser, seminar until a mobile phone rang out from That’s about it. Thank you to all who have Erin Lousberg, Iain Comerfold, Sarah the audience, sending the offending party helped me this year with encouragement Brice, Anastasia Yu, Kate Parham, Kiwi into a frenzy of panicked pocket slapping and and advice, and the occasional terse email Sun, Susan Christo, Siti Noordin and Sally fervent button pressing as he tried desperately reminding me to do things I should have Sun for all their hard work and enthusiasm to shut off the offending phone. Fortunately, done yesterday, but hadn’t. Please feel for the meeting. Also a BIG thank you I eventually managed to fi nd my phone free to contact me (berzins@unimelb. to all our sponsors: Miltenyi, Sapphire and to hit the mute button and the attention edu.au) should you wish to distribute any Bioscience, Jomar, UniSA, Adelaide Uni, thankfully shifted back to Prof Leonard. notices relating to immunology among the Invitrogen, Biacore, AdeLab Scientifi c, ASI/IgV membership. Merry Christmas DKSH, Geneworks, BD Biosciences and The IgV annual meeting was held this year to everyone and see you at this year’s ASI Genesearch. Without their generous fi nancial in Geelong. Previous meetings had been held Annual meeting in Adelaide! support the event each year could not be at Beechworth or the Yarra Valley and the held. IgV committee had hoped that the Geelong Stuart Berzins venue would see a new batch of immunology Councillor Michele Grimbaldeston people from the west of Melbourne attend and Councillor

ICB Online Manuscript Submission Sustaining Online manuscript submission for Membership Immunology and Cell Biology now available via: ASI Inc acknowledges the http://mc.manuscriptcentral.com/icb support of the following All manuscript submissions to ICB should sustaining member: in future be made online via this web site to speed up the reviewing and acceptance • Jomar Bioscience of manuscripts.

Gabrielle Belz, Editor-in-Chief Immunology and Cell Biology

15 ASI Inc. Newsletter December 2011 HONORARY SECRETARY’S NEWS

ASI annual meeting in Adelaide and 4 early career postdoc), adding up to a judging of these is still in progress while this The organisation of the Adelaide meeting little over $18,000. Money well spent! is written and the successful awardees will is in its fi nal stages. I’ve been told that Students be announced in the next newsletter. registration and abstract submission Pheh Ping Chang ACT numbers were going extremely well. Jie Chung Vic Council positions It promises to be a fantastic meeting. I Andreas Kupz Vic Nominations were called earlier this year for think the ASI annual scientifi c meeting Renee Robb Qld a number of Councillor positions in voting is a great way to end the year with the Rachael Terry NSW Council including ASI Executive, as well as opportunity to present and listen to the Courtney McDonald Vic the non-voting positions of VSP co-ordinator work that has been done over the year, Thi Vu NSW and the FIMSA Councillor. I’d like to take to catch up with and meet new friends Yogesh Jeelall ACT this opportunity to thank everybody who and collaborators. I’m looking forward Jonathan Chee Vic has offered their valuable time to ASI! Your to seeing you in Adelaide. Danushka Wijesundara ACT support is greatly appreciated! Royce Ng WA Travel bursaries to attend the ASI Louis Tsai Vic All positions have now been fi lled. Our new meeting in Adelaide Julia Marchingo Vic councillors are listed below. It is with great pleasure to announce the Koichi Ito Qld winners of this year’s student and early Sau Lee ACT This is my last contribution to the newsletter post doc travel bursaries to attend the Kok Fei Chan Vic as Hon Secretary. I say this with mixed annual meeting. ASI has traditionally Chin Lee Vic feelings. While I’m very much looking funded a number of students to attend Latasha Abeynaike Vic forward to that extra spare time on my hands the annual meeting. A couple of years Natalie Lorenz NZ (sorry, Rose), I’m also sad to leave the ASI ago we extended the bursaries to early Natalie Payne Vic Council and Executive. It has been a great career (up to 3 years) postdocs. Of experience for me and I enjoyed working with course applications are viewed relative Postdocs and for the Society very much. Welcome on to opportunity. So don’t worry if you are Tyani Chan NSW board to Rose Ffrench and Dale Godfrey to in your fi rst year of PhD ... you are not Motoko Koyama Qld the ASI Executive! directly competing against a Postdoc in Victor Peperzak Vic their third year! Stephane Chevrier Vic So long, and thanks for all the fi sh.

As always it was a diffi cult task for the Second Round International Travel Susanne Heinzel judging panel to select the best out of all Awards Hon Secretary the good applications. This year we were We again received a number of incredibly able to fund 24 applicants (20 students high quality applications for our ITAs. The

New Council members

Vice President Dale Godfrey University of Melbourne Hon Secretary Rose Ffrench Burnet Institute Councillor, NSW Marcel Batten Garvan Institute

VSP Co-ordinator Alejandro Lopez QIMR (re-nominated) FIMSA Councillor no applications received

ASI Secretariat PO Box 7108, Upper Ferntree Gully,Vic. 3156 Australia Tel: +61 3 9756 0128 Fax: +61 3 9753 6372 Email: [email protected] Offi ce hours: 8.30am – 4.30pm

16 ASI Inc. Newsletter December 2011

17 ASI Inc. Newsletter December 2011 What’s New in the World of Flow Cytometry Relating to Immunology? Update from CYTO2011 Baltimore, May 2011

Grace Chojnowski Queensland Institute of Medical Research

Where would immunology be without of Cytometry (ISAC) to help members better fl ow cytometry and would fl ow cytometry understand and hence design experiments have advanced as far without the world involving polychromatic cytometry. of immunology? Over the last few decades fl ow cytometry and fl uorescent Tutorials on polychromatic fl ow cytometry activated cell sorting (FACS) have as well as other tutorial material on fl ow had a close working relationship with cytometry applications will be available immunology. The relationship between on the new ISAC website http://www.isac- immunology and fl ow cytometry has net.org which should go live at the end of been a highly productive one with the 2011. partnership facilitating our knowledge in immunology and the development of Pre-congress courses and tutorials are run sophisticated instrumentation used in a couple of days before the main congress. disciplines other than immunology. These courses are designed with a range of topics to cater for researchers new to As instrumentation technology in cytometry as well as those of us who are fl ow cytometry advances so does the more experienced. One that would be Grace Chojnowski ability to gain valuable information of value to immunologists is the series on the immune system. The ability to of tutorials on Polychromatic Flow Immunity 33:326,2010). perform polychromatic fl ow cytometry Cytometry that were presented by Pratip K. using multiple excitation lasers and Chattopadhyay at CYTO 2011. These and Fellow ISAC councillor and organizer of simultaneous detection of 18 different more are planned as part of the CYTO 2012 the conference Andreas Radbruch gave a colours and beyond has given scientists pre congress course which will be held in brilliant presentation entitled “Cytometric a more detailed understanding of Leipzig, Germany, June 23–27, 2012 http:// tracking of Immunological Memory individual cells and how they relate cytoconference.org/CYTO . – It’s not in the Blood”. Andreas presented to one another and to different disease http://www.discoverymedicine.com/ his work that utilised new cytometric states. It has allowed us to isolate Pratip-K-Chattopadhyay/2009/06/29/the- technology that can be used to identify rare different subsets of cells and the colorful-future-of-cell-analysis-by-flow- cells and show how they regulate immune relevance of these subsets within the cytometry/ responses depending on their antigen immune system. Also samples are specifi city; also that many of these cells precious so the ability to acquire more Invivo Immunocytometry was one of the hot involved in orchestrating the responses and information on less samples is the goal topics at CYTO 2011 in the Immunology maintaining the immunological memory of many new methods. There are many stream with world class research being are found in niches within the lymphoid challenges, including: fi nding the most presented in the frontiers lecture series. tissue, infl amed tissue and bone marrow, but appropriate combination of labelled not in the blood. Taking that into account, antibodies with optimum staining index Total Internal Refl ection Fluorescence cytometric tracking of immunity offers the and, of course, the challenge of getting microscopy: TIRF was a technique exciting perspective to catch the cells on the correct spectral compensation. The used by Takashi Saito in his presentation the move and generate a dynamic picture of addition of new laser lines to bench-top Visualizing the Dynamics of Immune immunity (Koji Tokoyoda, Anja E. Hauser, instruments has eased this process by Synapse by TIRF. The immune synapse of Toshinori Nakayama & Andreas Radbruch allowing more fl uorochrome choices T cells and antigen presenting cells (APC) Organization of immunological memory and combinations but it can still be a the “T cell-APC interface” where dynamic by bone marrow stroma. 2010 Nat. Rev. challenge, especially when studying regulation of the activation process was Immunol Mar;10(3):193-200). cells which have low expression of analysed using the planar system and TIRF. receptors being studied. Qdots have The technology allows a researcher to study There were also a number of presentations also helped with compensation due changes occurring at the single molecule on the hot topic Inductively Coupled to their narrow emission spectra with level (for example changes occurring at Plasma Mass Spectrometry (ICP-MS) very little spectral overlap, resulting the plasma membrane) (refs: Yokusaka,T for Multi-parameter Cell Analysis. This in the requirement for little or no et al, Nat Immunol. 6:1253, 2005; Saito,T has the ability to perform well beyond 30 compensation. Introductory and more and Yokusaka,T. Curr.Opin Immunol parameter cytometry on single cells without advanced tutorials have been prepared 18:305,2006; Yokusaka,T et al, Immunity the need to perform complex compensation by the education committee of the 29:589,2008; Hasimoto_Tane,A et al. Mol. usually required when performing International Society for Advancement Cell.Biol 30:3421,2010; Yokusaka,T et al, conventional polychromatic fl ow cytometry.

18 ASI Inc. Newsletter December 2011

The technique is being led by Scott Tanner analysing the complex data also involving Australia has been successful with two from the University of Toronto, Canada. different types of clustering (Bendall SC, scholarships being granted; the benefi ts Individual cells are stained with stable Simonds EF, Qiu P, Amir ED, Krutzik PO, have been tremendous in facilitating isotypes attached to antibodies instead of Finck R, Bruggner RV, Melamed R, Trejo career goals and mentoring in cytometry. fl uorescent dyes or quantum dots (there are A, Ornatsky OI, Balderas RS, Plevritis SK, If you are interested in fi nding out more over 100 stable isotypes that are suitable Sachs K, Pe’er D, Tanner SD, Nolan GP. please go to http://www.isac-net.org for this type of analysis); Garry Nolan Single-cell mass cytometry of differential and his team in Stanford California have immune and drug responses across a human The Australasian Flow Cytometry made immense progress with applications hematopoietic continuum. Science. 2011 Group (AFCG) has also been very and advancing technology development. May 6; 332(6030):687-96). active with a revamp of our website A video of Garry is available on Youtube with updating of the clinical flow giving an excellent introductory explanation International Cytometry Certifi cation guidelines. The AFCG is an associated of ICP-MS (http://www.youtube.com/ Examination society of ISAC and together they are watch?v=hAZBg0naPBg). Both Garry ISAC (International Society for working on education material, up to Nolan and Scott Tanner presented work Advancement of Cytometry) and ICCS date information on courses, meeting that utilised ICP-MS and its superior (International Society for Clinical and teaching material to benefi t to both sensitivity; applying it to studies of immune Cytometry) have worked together to AFCG and ISAC members. http://www. responses or different infectious disease develop a certifi cation in cytometry, afcg.org.au/. The 2011 AFCG meeting states and the immune system (Ornatsky International Cytometry Certifi cation. The was held in Hobart in August with a O, Bandura D, Baranov V, Nitz M, Winnik motivation behind the establishment for large number of presentations relating MA, Tanner S. Highly multiparametric certifi cation in cytometry is to provide an to immunology. The presentations again analysis by mass cytometry.J Immunol internationally recognized minimal standard were world class from both international Methods . 2010 Sep 30;361(1-2):1-20; of competence. After years of preparation, and local presenters. Abstracts from Sean C. Bendallhttp://www.sciencemag. the examination for certifi cation is now the meeting are available on the AFCG org/content/332/6030/687.full - aff-1 et al. available and a number of candidates have website. The new revamped website Single-Cell Mass Cytometry of Differential already sat and passed the exam when it will also host educational material for Immune and Drug Responses Across a fi rst became available in June 2011. For our AFCG members so please go to the Human Hematopoietic Continuum Science more information on the certifi cation go to website and consider joining the AFCG 6 May 2011: Vol. 332 no. 6030 pp. 687- http://www.cytometrycertifi cation.org/ . to have access to resources. 696). ISAC Scholars Program Grace Chojnowski manages the fl ow With the introduction of mass The ISAC Scholars program is designed and imaging core facility at QIMR in multiparameter cytometry comes the to enhance the scientifi c and leadership Brisbane. Grace has been working in challenge of how does one analyse the experiences of emerging leaders in the fi eld the fi eld of cytometry since 1987 when volume and information generated from of cytometry. The ISAC Scholars program she was at Peter MacCallum Institute in this technology. Scott Tanner spoke about is designed for younger members, those Melbourne. Grace has been president of software that has been developed to analyse under the age of 40. The program provides the Australasian Flow Cytometry Group this data: Unsupervised Neural Networks opportunities for leadership training, (ISAC) and is presently an active member (UNN) and the clustering algorithms presentation opportunities, fi nancial on the AFCG executive committee. used to perform the analysis of samples support for membership of the Society, as Internationally Grace presently holds the acquired. Gary Nolan’s presentation well as other valuable mentoring activities. position as councillor on the International was using SPADE* and how its use in Society for Advancement of Cytometry (ISAC) where she is active in the education, membership services and associated societies committees. Contributions sought for the ASI online immunology quiz

As part of World Day of Immunology events, we have developed an online immunology quiz (see http://www.immunology.org.au/immquiz1.html) on the ASI website. This quiz is targeted at the general public, but it would be good to add a few more questions (especially some with an Australian fl avour), and maybe even add an “Advanced Level”, with questions that undergrad students might fi nd useful for revising for exams. All that’s needed now are the questions and answers.

If you would like to contribute any multiple choice questions for either the general quiz or an advanced version, please send them to Judith Greer at [email protected].

19 ASI Inc. Newsletter December 2011 THE ASI VISITING SPEAKER PROGRAM 2011

During 2011 we were very fortunate infected, and 10% of infected persons will Kirschner DE, Young D, Flynn JL. Tuberculosis: to have visits by Shane Crotty, Emil develop active disease. These studies may global approaches to a global disease. Curr Opin Unanue, Warren Leonard and Astrid lead to improved vaccine development or Biotechnol. 2010. Aug;21(4):524-31. Westendorf which were possible due immunotherapeutic strategies, as well as a Green AM, Mattila JT, Bigbee CL, Bongers KS, Lin PL, Flynn JL. CD4(+) regulatory T cells in a to the enthusiasm and work of their clear understanding of the host-pathogen cynomolgus macaque model of Mycobacterium hosts Stuart Berzins/Steve Turner, José interactions in tuberculosis.” tuberculosis infection. J Infect Dis. 2010 Aug Villadangos, Annie Xin and Hubertus 15;202(4):533-41. Jersmann. A big thank you for supporting Selected Publications Selected recent Marino S, Myers A, Flynn JL, Kirschner DE. the program. publications TNF and IL-10 are major factors in modulation Gideon HP, Flynn JL. Latent tuberculosis: what of the phagocytic cell environment in lung and At the same time, we would like to call the host “sees”? Immunol Res. 2011 Jun 30. lymph node in tuberculosis: a next-generation upon the membership to propose their Ford CB, Lin PL, Chase MR, Shah RR, two-compartmental model. J Theor Biol. 2010. candidates of interest for 2012. We have Iartchouk O, Galagan J, Mohaideen N, Ioerger Aug 21;265(4):586-98. TR, Sacchettini JC, Lipsitch M, Flynn JL, Lin PL, Flynn JL. Understanding latent already secured following visits: Fortune SM. Use of whole genome sequencing tuberculosis: a moving target. J Immunol. 2010 to estimate the mutation rate of Mycobacterium Jul 1;185(1):15-22. March 2012 tuberculosis during latent infection. Nat Genet. Russell DG, Barry CE 3rd, Flynn JL. Tuberculosis: Professor JoAnne L. Flynn 2011 May;43(5):482-6. what we don’t know can, and does, hurt us. Center for Vaccine Research Flynn JL, Chan J, Lin PL. Macrophages and control Science. 2010 May14; 328(5980):852-6. Microbiology & Molecular Genetics of granulomatous infl ammation in tuberculosis. University of Pittsburgh, Pennsylvania, Mucosal Immunol. 2011 May;4(3):271-8. August 2012 USA Diedrich CR, Flynn JL. HIV-1/mycobacterium Dr Pam Schwartzberg tuberculosis coinfection immunology: how does National Human Genome Research HIV-1 exacerbate tuberculosis? Infect Immun. Hosted by Michael Good, Gold Coast 2011 Apr;79(4):1407-17. Institute (Griffi th University) Mattila JT, Diedrich CR, Lin PL, Phuah J, NIH, Bethesda, MD, USA Flynn JL. Simian immunodeficiency virus- induced changes in T cell cytokine responses in Hosted by Stuart Tangye, Sydney (Garvan cynomolgus macaques with latent Mycobacterium Institute) tuberculosis infection are associated with More information will be provided in future timing of reactivation. J Immunol. 2011 Mar newsletters 15;186(6):3527-37.

“Tuberculosis kills ~2 million people per year, worldwide. We use various animal models to study T cell, macrophage, dendritic cell, cytokine and chemokine responses to Mycobacterium tuberculosis in the lungs. Our goal is to defi ne immune mechanisms that enhance resistance to this infection, as well as those that exacerbate pathology. We have funded projects on CD4 T cells, CD8 T cells, granuloma formation, and TNF-a in tuberculosis. We study both the acute and latent phase of infection, since it is estimated that one third of the world’s population is latently

20 ASI Inc. Newsletter December 2011 Travel Award Conference Reports

Mouse Genetics 2011 Colleen Elso St Vincent’s Institute, Victoria

In June 2011, hundreds of scientists working scale resources can be applied to make rapid on and with mouse genetics descended on progress in the search for the underlying the Omni Shoreham Hotel in Washington genetic mechanisms for both rare (progeria) DC for ‘Mouse Genetics 2011’. This and common (Type 2 diabetes and asthma) conference was jointly organised by the human diseases. The following days were International Mammalian Genome Society, packed with many stories of different mouse the Complex Trait Community and the models, resources and analysis tools. Genetics Society of America and themes covered included Disease Models, Large It was fascinating to see the advances since Scale Resources, Preclinical Translation and I last attended the IMGS meeting 10 years Systems Genetics. ago when results from the fi rst large-scale ENU screens were just being reported The conference began with an optional and support for the Collaborative Cross Bioinformatics Workshop focused on was gaining momentum. Now the fi eld analysing next-generation sequence data. has moved on to huge large-scale ‘mouse As a recipient of a conference scholarship, clinics’ to phenotype many of the mutants I was given the opportunity to attend this (including intensive immunological workshop and I now know much more screens), and results from some of the about the process, the tools and terminology Collaborative Cross strains are shedding used and will be much more confi dent light on phenotypes as varied as host discussing my sams and bams and wiggles response to infection and male infertility. with collaborators in the future! (Thank A recurring theme was the utility of next- Colleen and her children at the goodness for bioinformaticians with lots gen sequence for mapping mutations both Washington Monument of computing power …) The rest of the in mouse models and human patients with day was an opportunity for students and rare mutations. postdocs (including me) to present their data. It was a fantastic session ranging from As well as the scientifi c value of the infectious diseases to autoimmunity, to diet conference, it was a great opportunity to and cancer, using a wide range of mouse see a new part of the world: the conference genetic techniques and resources. dinner was followed by a Moonlight Tour of Washington DC, where we drove past Contributions The fi rst day of the main program the very historic and famous landmarks of highlighted progress being made in some the National Mall and surrounding areas, sought for the of the large-scale projects such as the with time to visit the Lincoln Memorial. It availability of genome sequence data was also great to meet some new colleagues ASI Newsletter for 17 classical and wild-derived mouse and reconnect with some old ones resulting strains; the Collaborative Cross (a multi- in what I hope will be a very fruitful You could win parental recombinant inbred cross to aid in collaboration. Thanks to ASI for their mapping QTLs); large mouse phenotyping contribution to my trip. The information $200 !! efforts; and how exome sequencing of and resources I have gained from this mouse models is shedding light on human meeting will help to move us forward as we Deadline for the diseases. Francis Collins gave the keynote continue to use mouse models to search for address, giving examples from his own genes underlying autoimmune diseases. next issue : laboratory to highlight how these large- 1st February 2012

Please email your contributions to the Secretariat by the above date. [email protected]

21 ASI Inc. Newsletter December 2011

17th Germinal Centre Conference Ingela Vikstrom and Katja Lüthje Walter & Eliza Hall Institute, Melbourne

Two individual post doc international occasions. The surroundings were beautiful this process. Furthermore, she described the travel awards from ASI allowed us and we were tempted to go for strolls on development of gut-associated lymphoid to attend the 17th Germinal Centre the small walk paths, however, these were tissues, SILT and colonic patches. A couple Conference (GCC) in Birmingham, unfortunately assigned to golfers only and of excellent presentations followed, dealing UK, 4–8 September, 2011. After the we were restricted to doing circles around with the generation of lymphoid organs: meeting, we parted ways and visited the building. innate lymphoid cells (ILC), lymphoid tissue several labs. organiser cells (LTO), T zone reticular cells The conference started on Sunday evening (TRC) and isolated lymphoid follicles (ILF). The GCC has a long and well-established with an inspiring talk by Prof. Carola All these amazing results were presented with history, spanning over four decades. Vinuesa (The John Curtin School of accompanying images. A great overview of This year’s meeting promised to Medical Research) focussing on regulation this important fi eld of immunology and an bring us the latest research covering of T follicular helper cell formation and excellent start to the conference. In addition, lymphoid development, chemokines, homeostasis. Drinks and an opportunity the fi rst of the poster sessions commenced plasma cells, infection, transformation, for everyone to catch up followed the that evening and then continued throughout ageing, T follicular helper cells and presentation, the jet lag, however, forced us the conference; the interest in the posters germinal centres. The conference to a relatively early rest. was very high and there were many vivid was of intermediate size, with around discussions taking place until late into the 180 participants, many with a strong The fi rst full day of the conference addressed evening. international standing. We found that a the development of lymphoid tissues. Reina big advantage with this size of meeting Mebius from the Netherlands opened The second day of the conference covered was that everything was limited to this session with an excellent talk about topics such as plasma cells, ageing and cell one room, with no parallel sessions, lymphoid tissue inducer cells (Lti cells). transformation. The plasma cell session in this way we did not miss anything This talk focussed on their dependence on started off with Dr Facundo Batista (London of interest. The conference was held lymphotoxins (LTαβ) and the chemokine Research Institute) showing us his impressive at The Belfry, a prestigious golf resort receptors CXCR5 and CXCL13, as well as imaging of the dynamic changes that occur having hosted The Ryder Cup on four the important infl uence of retinoic acid on in B cells during their activation. Later in the same session, Dr Ulf Klein (Columbia University) gave an interesting talk on “New insights into the germinal centre exit” focussing on NFkB transcription factor subunits in GC B cell development. In the session on ageing, Dr Michael Cancro (University of Pennsylvania) gave a talk on how modulation of BLyS family member expression can govern plasma cell survival. Prof. Harald Stein (Charité University Medicine Berlin) and Prof. Ralph Küppers (University of Duisburg-Essen) both gave good overviews of the relationships of germinal centres and memory B cells to B cell malignancies.

The third day of the conference covered germinal centres and infection/vaccination. Dr Mark Schlomchik (Yale University), Dr Gabriel Victora (Massachusetts Institute of Technology) and Dr Kai-Michael Toellner (University of Birmingham) gave very interesting talks on GC selection. In addition, Prof. Michael Meyer-Hermann (Helmholtz Centre for Infection Research) gave an intriguing demonstration of an in silico model of the same process. The infection/vaccination session started with Katja Luetje (left) & Ingela Vikstrom at the 17th Germinal Centre Conference Dr Adam Cunningham (University of

22 ASI Inc. Newsletter December 2011

Birmingham) explaining the regulation Lab visit by Ingela: of the extrafollicular and germinal centre After the conference fi nished, I travelled to An invitation and a request responses to Salmonella. Later on, Dr Cambridge to catch up with a dear friend to all ASI members Michelle Wykes (Queensland Institute of of mine and fellow immunologist, Jen to contribute copy that they think Medical Research) talked about the role of Walker (formerly WEHI). I also took the might be interesting, useful, historical, humorous or thought dendritic cells during malaria infection. The opportunity to visit Dr Martin Turner’s lab at provoking. day fi nished with a delicious gala dinner the Lymphocyte Signalling and Development followed by entertainment consisting of a programme at the Babraham Institute, where ¾ We invite our student band performing ABBA songs to the delight I gave a presentation of my research. It was a membership to voice their of some but not as much others … by the great day, where I got to interact and exchange views on issues that interest end of it, most people were swinging their ideas with several brilliant researchers. or directly concern them. legs on the dance fl oor anyway! Lab visit by Katja: ¾ It’s our newsletter, so let’s The closing session on the last day of the My additional trip led me to Germany, support it and strive to make it conference discussed the important cells starting in the north – Hamburg. There I even better. that regulate B cell responses, T follicular visited the Bernhard-Nocht-Insitute for helper cells. In three independent talks, the Tropical Medicine (BNI). Afterwards I ¾ The ASI newsletter comes out new subset of CD4+ T cells, the “follicular proceeded south to Munich. Unfortunately, 4 times a year and we welcome regulatory T cell (TFR)”, was introduced. or luckily – depending on your point of your contributions. These cells are like T follicular helper cells view – the Oktoberfest had started and AND YOU COULD WIN (TFH) dependent on the expression of the whole city was celebrating beer. But I ¾ $200 FOR THE BEST BCL6; but they co express FOXP3 and act headed fi rst to the Institute of Molecular ARTICLE PUBLISHED IN like regulatory T cells – in this particular Immunology of the HelmholtzZentrum and THE NEWSLETTER! circumstance by controlling the germinal the following morning to the Institute for centre response. Thus, while the density of Immunology at the Ludwig-Maximilians TFH cells inside a germinal centre declines University. I enjoyed each of my visits, over time, the density of TFR cells increases. which were jam-packed and led to a bunch With this excellent fi nale, and following of new impressions. the great time we had at the conference, we split up to proceed with our independent Taken together, we had a gorgeous trip full lab visits. of experiences and we really appreciate the support from ASI. Thank you!

UPCOMING CONFERENCES

5th Congress of the Federation of TLROZ 2012 15th Biennial Meeting of the European Immunological Societies of Asia May 2–4, 2012 Society for Immunodefi ciencies Oceania (FIMSA 2012) –Translational Melbourne, Australia October 3–6, 2012 Immunology in Health & Science www.TLROZ2012.com Florence, Italy March 14–17, 2012 www.kenes.com/esid New Delhi, India Immunology 2012 fi msa@fi msa2012.com May 4–8, 2012 3rd International Conference on www.fi msa2012.com Boston, USA Regulatory T Cells & Th Subsets www.IMMUNOLOGY_2012TM.com and Clinical Application in Human V World Asthma & COPD Forum [email protected] Disease (China Tregs/Th Subsets 2012 April 21–24, 2012 conference) New York, USA 15th International Congress of October 13–16, 2012 [email protected] Immunology Shanghai, China www.wipocis.org August 22–27, 2013 http://www.chinatregs.com Rome, Italy 4th Australasian Vaccines & Immuno- [email protected] The Walter and Eliza Hall therapeutics Development Meeting www.ici2013.org May 2–4, 2012 Institute of Medical Research Brisbane, Australia WEHI Seminars on the Web: www.avid2012.org www.wehi.edu/seminars/

23 ASI Inc. Newsletter December 2011

2011 European Multidisciplinary Congress Conference Stockholm, Sweden, 23-27 September 2011 Alison West Peter MacCallum Cancer Centre, Melbourne

For two weeks before the 2011 European of ‘mouse hospitals’ was also discussed, a about the merits of circulating tumour cell Multidisciplinary Congress Conference system allowing constant cross referencing biology and subsequent application for in Stockholm, I checked the Swedish between the patient and the mouse, diagnosis and drug responsiveness. It is weather reports. It was not looking allowing better tailoring of drugs, doses and amazing how precise tumour cell detection promising. Nonetheless on September combinations. Finally the nurses and patients in the blood can be (down to 1 tumour cell 21 I packed up in an Australian Spring explained the all-important, albeit careful, per 7.5mL), although it is a while until this and headed to a Scandinavian Autumn. use of media to educate the public on new technology reaches regular practice in the “I am doing it for the good of cancer therapies and technologies as they come to clinic. Finally, the opportunity to present immunology” I told myself. But over the fore. After this truly multidisciplinary my own data in the ‘proffered papers: basic the next few days I realised there was seminar I felt ready to take on the rest of science/translation research’ session was a nowhere else I would rather be, both the conference and to fi t my piece of tumour highlight in hindsight, though at the time it for myself and for the good of cancer immunology data into the greater cancer was somewhat intimidating! The feedback immunology! research puzzle. I received after my talk was invaluable, as was the enforced ‘outside the box’ thinking The conference was of epic proportions. In between wandering around the many required to present my work to such a This is a world where the main hall beautiful harbours and islands that translation-orientated audience. seated about 3,000 people, there were 16 make up Stockholm, I attended many concurrent sessions covering 33 tracks, interesting sessions. The fi rst highlight I left Stockholm in awe of many things. and each exhibitor’s booth looked more was a session characterising the role of the Firstly of how many ways there are to eat like a cafe – complete with plasma TVs, microenvironment in lymphoma chaired herring, and secondly of how broad the fi eld couches and coffee machines operated by by Paolo Ghia (Vita-Salute San Raffaele of cancer research is and what a special niche specially hired baristas – than a display University, Italy) and Ralf Küppers tumour immunology has in the middle of it table. Needless to say, I followed the (University of Duisburg-Essen, Germany). In all. I feel very privileged to have attended the other 15,931 delegates into the exhibitors’ this session cancer biologists, immunologists 2011 European Multidisciplinary Congress area each morning to begin the day with and clinicians came together to discuss the Conference and am so appreciative of the ASI a free coffee. cellular interactions occurring in lymphoid International Travel Award I received for this organs during lymphomagenesis and how trip. I would encourage all ASI students to Thankfully the science was just as epic tumour cells can be dislodged from their apply for this award and to fi nd a conference as the conference. The 2011 European comfortable niche and destroyed. Another which helps you to think outside your square Multidisciplinary Congress Conference highlight was an informative and passionate and get creative. After all, great science is represented a conglomerate of clinicians, debate between Caroline Dive (Paterson formed from great collaborations. And you nurses, surgeons, basic scientists and even Institute for Cancer Research), Stefan Sleijfer might just get to eat a herring somewhere patients from all parts of the world (116 (Erasmus University Medical Centre) and along the way … countries to be exact). This wonderfully Klaus Pantel (University of Hamburg) diverse mix of people made for a broad meeting, which forced delegates to think outside their own square and apply their knowledge to the greater cause – fi nding a cure for cancer.

The meeting began with a ‘bigger picture’ keynote address, given by a wonderfully mixed bag panel, optimistically describing their combined perfect vision for the future: truly personalised cancer therapy. First we heard how clinicians are gaining better techniques to take tumour biopsies at diagnosis from both the primary site and from the blood. Then the scientists explained how high throughput technology is now allowing genetic, immunological and drug sensitivity testing to be carried out on these samples. The controversial idea

24 ASI Inc. Newsletter December 2011 Publications List Congratulations to ASI members who have published their following work in the last three months (articles with an ePub date between July and September 2011)

Mitchell AJ, Pradel LC, Chasson L, Van Rooijen N, Nantakomol D, Dondorp AM, Krudsood S, alterations to murine brain tissue induced Grau GE, Hunt NH, Chimini G. Technical advance: Udomsangpetch R, Pattanapanyasat K, Combes V, Grau by formalin fixation: implications for autofl uorescence as a tool for myeloid cell analysis. GE et al. Circulating red cell-derived microparticles biospectroscopic imaging and mapping studies J Leukoc Biol 2010; 88(3): 597. in human malaria. J Infect Dis 2011; 203(5): 700. of disease pathogenesis. Analyst 2011; 136(14): 2941. Mfonkeu JB, Gouado I, Kuate HF, Zambou O, Combes Tovar C, Obendorf D, Murchison EP, Papenfuss AT, V, Grau GE, Zollo PH. Biochemical markers of Kreiss A, Woods GM. Tumor-specifi c diagnostic Trieu A, Kayala MA, Burk C, Molina DM, Freilich nutritional status and childhood malaria severity marker for transmissible facial tumors of tasmanian DA, Richie TL, Baldi P et al. Sterile protective in Cameroon. Br J Nutr 2010; 104(6): 886. devils: immunohistochemistry studies. Vet Pathol immunity to malaria is associated with a panel of 2011; 48(6): 1195. novel P. falciparum antigens. Mol Cell Proteomics Carpintero R, Gruaz L, Brandt KJ, Scanu A, Faille D, 2011; 10(9): M111 007948. Combes V, Grau GE et al. HDL interfere with the El-Assaad F, Hempel C, Combes V, Mitchell AJ, Ball HJ, binding of T cell microparticles to human monocytes Kurtzhals JA, Hunt NH et al. Differential microRNA Sheng YH, Lourie R, Linden SK, Jeffery PL, Roche to inhibit pro-infl ammatory cytokine production. expression in experimental cerebral and noncerebral D, Tran TV, Png CW et al. The MUC13 cell-surface PLoS One 2010; 5(7): e11869. malaria. Infect Immun 2011; 79(6): 2379. mucin protects against intestinal infl ammation by inhibiting epithelial cell apoptosis. Gut 2011; Jambou R, Combes V, Jambou MJ, Weksler BB, Couraud Jeffery P, McDonald V, Tippett E, McGuckin M. Ghrelin 60(12): 1661. PO, Grau GE. Plasmodium falciparum adhesion in gastrointestinal disease. Mol Cell Endocrinol 2011; on human brain microvascular endothelial cells 340(1): 35. Mollah ZU, Wali J, McKenzie MD, Krishnamurthy B, Graham KL, Fynch S, Szanyi J et al. The pro- involves transmigration-like cup formation and McNally A, Hill GR, Sparwasser T, Thomas R, apoptotic BH3-only protein Bid is dispensable induces opening of intercellular junctions. PLoS Steptoe RJ. CD4+CD25+ regulatory T cells control for development of insulitis and diabetes in Pathog 2010; 6(7): e1001021. CD8+ T-cell effector differentiation by modulating the non-obese diabetic mouse. Apoptosis 2011; Hee L, Dinudom A, Mitchell AJ, Grau GE, Cook DI, IL-2 homeostasis. Proc Natl Acad Sci U S A 2011; 16(8): 822. Hunt NH, Ball HJ. Reduced activity of the epithelial 108(18): 7529. Ghadersohi A, Sharma S, Zhang S, Azrak RG, sodium channel in malaria-induced pulmonary Hubert FX, Kinkel SA, Davey GM, Phipson B, Mueller Wilding GE, Manjili MH, Li F. Prostate-derived oedema in mice. Int J Parasitol 2011; 41(1): 81. SN, Liston A, Proietto AI et al. Aire regulates the Ets transcription factor (PDEF) is a potential McQuillan JA, Mitchell AJ, Ho YF, Combes V, Ball HJ, transfer of antigen from mTECs to dendritic cells prognostic marker in patients with prostate Golenser J, Grau GE et al. Coincident parasite and for induction of thymic tolerance. Blood 2011; cancer. Prostate 2011; 71(11): 1178. CD8 T cell sequestration is required for development 118(9): 2462. Kmieciak M, Basu D, Payne KK, Toor A, Yacoub of experimental cerebral malaria. Int J Parasitol Lee S, Fernandez S, French M, Price P. Chemokine A, Wang XY, Smith L et al. Activated NKT cells 2011; 41(2): 155. receptor expression on dendritic cells is normal in and NK cells render T cells resistant to myeloid- Pankoui Mfonkeu JB, Gouado I, Fotso Kuate H, Zambou HIV-infected patients with a stable response to art, derived suppressor cells and result in an effective O, Amvam Zollo PH, Grau GE, Combes V. Elevated but chemokine levels remain elevated. J Med Virol adoptive cellular therapy against breast cancer cell-specifi c microparticles are a biological marker 2011; 83(7): 1128. in the FVBN202 transgenic mouse. J Immunol for cerebral dysfunctions in human severe malaria. Lim A, Amini A, D’Orsogna LJ, Rajasuriar R, Kramski 2011; 187(2): 708. PLoS One 2010; 5(10): e13415. M, Lewin SR, Purcell DF et al. Antibody and B-cell Haque A, Best SE, Ammerdorffer A, Desbarrieres Wassmer SC, Moxon CA, Taylor T, Grau GE, Molyneux responses may control circulating lipopolysaccharide L, de Oca MM, Amante FH, de Labastida Rivera ME, Craig AG. Vascular endothelial cells cultured in patients with HIV infection. AIDS 2011; 25(11): F et al. Type I interferons suppress CD4 T-cell- from patients with cerebral or uncomplicated 1379. dependent parasite control during blood-stage malaria exhibit differential reactivity to TNF. Cell Barbier M, Faille D, Loriod B, Textoris J, Camus C, Plasmodium infection. Eur J Immunol 2011; Microbiol 2011; 13(2): 198. Puthier D, Flori L et al. Platelets alter gene expression 41(9): 2688. van der Heyde HC, Gramaglia I, Combes V, George TC, profi le in human brain endothelial cells in an in Lahoud MH, Ahmet F, Kitsoulis S, Wan SS, Vremec Grau GE. Flow cytometric analysis of microparticles. vitro model of cerebral malaria. PLoS One 2011; D, Lee CN, Phipson B et al. Targeting antigen Methods Mol Biol 2011; 699: 337. 6(5): e19651. to mouse dendritic cells via Clec9A induces Hoyne GF, Chapman G, Sontani Y, Pursglove SE, Hoyne GF. Mechanisms that regulate peripheral potent CD4 T cell responses biased toward a Dunwoodie SL. A cell autonomous role for the Notch immune responses to control organ-specific follicular helper phenotype. J Immunol 2011; ligand Delta-like 3 in alphabeta T-cell development. autoimmunity. Clin Dev Immunol 2011; 2011: 187(2): 842. Immunol Cell Biol 2011; 89(6): 696. 294968. Jawahar MC, Sari CI, Wilson YM, Lawrence AJ, Lo L, McLennan SV, Williams PF, Bonner J, Chowdhury Effects of perindopril-indapamide on left ventricular Brodnicki T, Murphy M. Audiogenic seizure S, McCaughan GW, Gorrell MD et al. Diabetes is a diastolic function and mass in patients with type proneness requires the contribution of two progression factor for hepatic fi brosis in a high fat fed 2 diabetes: the ADVANCE Echocardiography susceptibility loci in mice. Neurogenetics 2011; mouse obesity model of non-alcoholic steatohepatitis. Substudy. J Hypertens 2011; 29(7): 1439. 12(3): 253. J Hepatol 2011; 55(2): 435. Tan DB, Lim A, Yong YK, Ponnampalavanar S, Omar Loukas A, Gaze S, Mulvenna JP, Gasser RB, McGee HM, Malley RC, Muller HK, Woods GM. S, Kamarulzaman A, French MA et al. TLR2-induced Brindley PJ, Doolan DL, Bethony JM et al. Neonatal exposure to UVR alters skin immune system cytokine responses may characterize HIV-infected Vaccinomics for the major blood feeding development, and suppresses immunity in adulthood. patients experiencing mycobacterial immune helminths of humans. OMICS 2011; 15(9): 567. Immunol Cell Biol 2011; 89(7): 767. restoration disease. AIDS 2011; 25(12): 1455. Ebrahimi D, Anwar F, Davenport MP. APOBEC3 Hoyne GF, Flening E, Yabas M, Teh C, Altin JA, Randall Yao TW, Kim WS, Yu DM, Sharbeen G, McCaughan has not left an evolutionary footprint on the K, Thien CB et al. Visualizing the role of Cbl-b in GW, Choi KY, Xia P et al. A novel role of dipeptidyl HIV-1 genome. J Virol 2011; 85(17): 9139. control of islet-reactive CD4 T cells and susceptibility peptidase 9 in epidermal growth factor signaling. Pleasance J, Wiedosari E, Raadsma HW, Meeusen to type 1 diabetes. J Immunol 2011; 186(4): 2024. Mol Cancer Res 2011; 9(7): 948. E, Piedrafi ta D. Resistance to liver fl uke infection Begg DJ, de Silva K, Carter N, Plain KM, Purdie A, Moxon CA, Grau GE, Craig AG. Malaria: modifi cation in the natural sheep host is correlated with Whittington RJ. Does a Th1 over Th2 dominancy of the red blood cell and consequences in the human a type-1 cytokine response. Parasite Immunol really exist in the early stages of Mycobacterium host. Br J Haematol 2011. 2011; 33(9): 495. avium subspecies paratuberculosis infections? Hackett MJ, McQuillan JA, El-Assaad F, Aitken JB, Plain KM, de Silva K, Earl J, Begg DJ, Purdie AC, Immunobiology 2011; 216(7): 840. Levina A, Cohen DD, Siegele R et al. Chemical Whittington RJ. Indoleamine 2,3-dioxygenase,

25 ASI Inc. Newsletter December 2011

tryptophan catabolism, and Mycobacterium Every AL, Ng GZ, Skene CD, Harbour SN, Walduck cerebral than noncerebral malaria and is reversed by avium subsp. paratuberculosis: a model for AK, McGuckin MA, Sutton P. Localized suppression erythropoietin. Am J Pathol 2011; 179(4): 1939. chronic mycobacterial infections. Infect Immun of infl ammation at sites of Helicobacter pylori Ramsland PA, Farrugia W, Bradford TM, Sardjono CT, 2011; 79(9): 3821. colonization. Infect Immun 2011; 79(10): 4186. Esparon S, Trist HM, Powell MS et al. Structural basis Iglesias MC, Almeida JR, Fastenackels S, van Hagn M, Sontheimer K, Dahlke K, Brueggemann S, for Fc gammaRIIa recognition of human IgG and Bockel DJ, Hashimoto M, Venturi V, Gostick E et al. Kaltenmeier C, Beyer T, Hofmann S et al. Human B cells formation of infl ammatory signaling complexes. J Escape from highly effective public CD8+ T-cell differentiate into granzyme B-secreting cytotoxic B Immunol 2011; 187(6): 3208. clonotypes by HIV. Blood 2011; 118(8): 2138. lymphocytes upon incomplete T-cell help. Immunol Rao N, Butcher CM, Lewis ID, Ross DM, Melo Layton RC, Petrovsky N, Gigliotti AP, Pollock Cell Biol 2011. JV, Scott HS, Bardy PG et al. Clonal and lineage Z, Knight J, Donart N, Pyles J et al. Delta inulin French MA, Oliver BG, Elliott JH, Price P. Plasma analysis of somatic DNMT3A and JAK2 mutations polysaccharide adjuvant enhances the ability biomarkers in the prediction and diagnosis of in a chronic phase polycythemia vera patient. Br J of split-virion H5N1 vaccine to protect against tuberculosis-associated immune reconstitution Haematol 2011. lethal challenge in ferrets. Vaccine 2011; 29(37): infl ammatory syndrome. AIDS 2011; 25(13): 1676. Wang N, Strugnell R, Wijburg O, Brodnicki T. 6242. Kreiss A, Cheng Y, Kimble F, Wells B, Donovan S, Belov Measuring bacterial load and immune responses Mattarollo SR, Yong M, Gosmann C, Choyce A, K, Woods GM. Allorecognition in the Tasmanian devil in mice infected with Listeria monocytogenes. J Vis Chan D, Leggatt GR, Frazer IH. NKT cells inhibit (Sarcophilus harrisii), an endangered marsupial Exp 2011; (54). antigen-specifi c effector CD8 T cell induction species with limited genetic diversity. PLoS One Pelosi A, Shepherd R, De Guzman G, Hamill J, Meeusen to skin viral proteins. J Immunol 2011; 187(4): 2011; 6(7): e22402. E, Sanson G, Walmsley AM. The Release and Induced 1601. Campo JJ, Whitman TJ, Freilich D, Burgess TH, Immune Responses of a Plant-made and Delivered McInnes N, Sadlon TJ, Brown CY, Pederson S, Martin GJ, Doolan DL. Toward a surrogate marker Antigen in the Mouse Gut. Curr Drug Deliv 2011. Beyer M, Schultze JL, McColl S et al. FOXP3 and of malaria exposure: modeling longitudinal antibody Zhang Q, Sturgill JL, Kmieciak M, Szczepanek K, FOXP3-regulated microRNAs suppress SATB1 measurements under outbreak conditions. PLoS One Derecka M, Koebel C, Graham LJ et al. The role of Tyk2 in breast cancer cells. Oncogene 2011. 2011; 6(7): e21826. in regulation of breast cancer growth. J Interferon Moghaddam AE, Gartlan KH, Kong L, Sattentau Mohanasundaram D, Drogemuller C, Brealey J, Jessup Cytokine Res 2011; 31(9): 671. QJ. Reactive carbonyls are a major Th2- CF, Milner C, Murgia C, Lang CJ et al. Ultrastructural Sutton P. Vaccinating against Helicobacter pylori: inducing damage-associated molecular pattern analysis, zinc transporters, glucose transporters dissecting the mechanism. Gastroenterology 2011; generated by oxidative stress. J Immunol 2011; and hormones expression in New world primate 141(4): 1149. 187(4): 1626. (Callithrix jacchus) and human pancreatic islets. Gen Comp Endocrinol 2011; 174(2): 71. Wilkinson R, Woods K, D’Rozario R, Prue R, Vari F, Odobasic D, Gan PY, Summers SA, Semple Hardy MY, Dong Y et al. Human kallikrein 4 signal TJ, Muljadi RC, Iwakura Y, Kitching AR et Barry AE, Trieu A, Fowkes FJ, Pablo J, Kalantari- peptide induces cytotoxic T cell responses in healthy al. Interleukin-17A promotes early but Dehaghi M, Jasinskas A, Tan X et al. The Stability donors and prostate cancer patients. Cancer Immunol attenuates established disease in crescentic and Complexity of Antibody Responses to the Major Immunother 2011. glomerulonephritis in mice. Am J Pathol 2011; Surface Antigen of Plasmodium falciparum Are 179(3): 1188. Associated with Age in a Malaria Endemic Area. Mol Ascierto ML, De Giorgi V, Liu Q, Bedognetti D, Spivey Cell Proteomics 2011; 10(11): M111 008326. TL, Murtas D, Uccellini L et al. An immunologic Saggu R, Faille D, Grau GE, Cozzone PJ, Viola portrait of cancer. J Transl Med 2011; 9: 146. A. In the eye of experimental cerebral malaria. Cashin K, Roche M, Sterjovski J, Ellett A, Gray LR, Am J Pathol 2011; 179(3): 1104. Cunningham AL, Ramsland PA et al. Alternative Faille D, El-Assaad F, Mitchell AJ, Alessi MC, Chimini G, Fusai T, Grau GE et al. Endocytosis and Pomie C, Vicente R, Vuddamalay Y, Lundgren coreceptor requirements for effi cient CCR5- and CXCR4-mediated HIV-1 entry into macrophages. intracellular processing of platelet microparticles by BA, van der Hoek M, Enault G, Kagan J et brain endothelial cells. J Cell Mol Med 2011. al. Autoimmune regulator (AIRE)-defi cient J Virol 2011; 85(20): 10699. CD8+CD28low regulatory T lymphocytes fail Idowu MO, Kmieciak M, Dumur C, Burton RS, Grimes Yekollu SK, Thomas R, O’Sullivan B. Targeting to control experimental colitis. Proc Natl Acad MM, Powers CN, Manjili MH. CD44(+)/CD24(-/low) Curcusomes to Infl ammatory Dendritic Cells Inhibits Sci U S A 2011; 108(30): 12437. cancer stem/progenitor cells are more abundant in NF-{kappa}B and Improves Insulin Resistance in Obese Mice. Diabetes 2011; 60(11): 2928. Rogers N, Stephenson M, Kitching A, Horowitz triple-negative invasive breast carcinoma phenotype J, Coates P. Amelioration of renal ischaemia- and are associated with poor outcome. Hum Pathol Hahn CN, Chong CE, Carmichael CL, Wilkins EJ, reperfusion injury by liposomal delivery of 2011. Brautigan PJ, Li XC, Babic M et al. Heritable GATA2 curcumin to renal tubular epithelial and antigen Nozaki Y, Nikolic-Paterson DJ, Yagita H, Akiba mutations associated with familial myelodysplastic presenting cells. Br J Pharmacol 2011. H, Holdsworth SR, Kitching AR. Tim-1 promotes syndrome and acute myeloid leukemia. Nat Genet 2011; 43(10): 1012. Gong J, Jaiswal R, Mathys JM, Combes V, Grau GE, cisplatin nephrotoxicity. Am J Physiol Renal Physiol Bebawy M. Microparticles and their emerging 2011; 301(5): F1098. Fromm PD, Gottlieb D, Bradstock KF, Hart DN. role in cancer multidrug resistance. Cancer Beyer M, Thabet Y, Muller RU, Sadlon T, Classen Cellular therapy to treat haematological and other Treat Rev 2011. S, Lahl K, Basu S et al. Repression of the genome malignancies: progress and pitfalls. Pathology 2011; 43(6): 605. Tate MD, Brooks AG, Reading PC. Specifi c sites organizer SATB1 in regulatory T cells is required of N-linked glycosylation on the hemagglutinin for suppressive function and inhibition of effector O’Sullivan BJ, Pai S, Street S, An X, MacDonald KP, of H1N1 subtype infl uenza A virus determine differentiation. Nat Immunol 2011; 12(9): 898. Wong M, Strutton G et al. Immunotherapy with sensitivity to inhibitors of the innate immune Gebhardt T, Whitney PG, Zaid A, Mackay LK, Brooks costimulatory dendritic cells to control autoimmune system and virulence in mice. J Immunol 2011; AG, Heath WR, Carbone FR et al. Different patterns infl ammation. J Immunol 2011; 187(8): 4018. 187(4): 1884. of peripheral migration by memory CD4+ and CD8+ Ampawong S, Combes V, Hunt NH, Radford J, Chan- Wines BD, Ramsland PA, Trist HM, Gardam S, T cells. Nature 2011; 477(7363): 216. Ling T, Pongponratn E, Grau GE. Quantitation of brain Brink R, Fraser JD, Hogarth PM. Interaction of Sontani Y, Chapman G, Papathanasiou P, Dunwoodie edema and localisation of aquaporin 4 expression in human, rat, and mouse immunoglobulin A (IgA) S, Goodnow CC, Hoyne GF. Cooperation between relation to susceptibility to experimental cerebral with Staphylococcal superantigen-like 7 (SSL7) somatic Ikaros and Notch1 mutations at the inception malaria. Int J Clin Exp Pathol 2011; 4(6): 566. decoy protein and leukocyte IgA receptor. J Biol of T-ALL. Leuk Res 2011; 35(11): 1512. Apte SH, Groves PL, Roddick JS, V PdH, Doolan DL. Chem 2011; 286(38): 33118. Hart PH, Gorman S, Finlay-Jones JJ. 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26 ASI Inc. Newsletter December 2011 bones include double-positive CD8+CD4+ T cells: de Veer M, Meeusen E. New developments in vaccine Sedegah M, Tamminga C, McGrath S, House B, evidence from myeloma-bearing mouse model. J research--unveiling the secret of vaccine adjuvants. Ganeshan H, Lejano J, Abot E et al. Adenovirus Immunol 2011; 187(8): 3987. Discov Med 2011; 12(64): 195. 5-vectored P. falciparum vaccine expressing CSP Guo C, Yoon HS, Franklin A, Jain S, Ebert A, Cheng Hoorens P, Rinaldi M, Mihi B, Dreesen L, Grit G, and AMA1. Part A: safety and immunogenicity 6(10): HL, Hansen E et al. CTCF-binding elements mediate Meeusen E, Li RW et al. Galectin-11 induction in the in seronegative adults. PLoS One 2011; e24586. control of V(D)J recombination. Nature 2011; gastrointestinal tract of cattle following nematode 477(7365): 424. and protozoan infections. Parasite Immunol 2011; Tamminga C, Sedegah M, Regis D, Chuang I, Brown JA, Roberts TL, Richards R, Woods R, Birrell 33(12): 669. Epstein JE, Spring M, Mendoza-Silveiras J et al. Adenovirus-5-vectored P. falciparum vaccine G, Lim YC, Ohno S et al. A Novel Role for hSMG-1 Jaiswal R, Gong J, Sambasivam S, Combes V, Mathys expressing CSP and AMA1. Part B: safety, in Stress Granule Formation. Mol Cell Biol 2011; JM, Davey R, Grau GE et al. Microparticle-associated immunogenicity and protective efficacy of 31(22): 4417. nucleic acids mediate trait dominance in cancer. the CSP component. PLoS One 2011; 6(10): FASEB J 2011. Sheng YH, Hasnain SZ, Florin TH, McGuckin MA. e25868. Mucins in Ibd and Colorectal Cancer. J Gastroenterol Turner ML, Schnorfeil FM, Brocker T. MicroRNAs Fernandez S, Tanaskovic S, Helbig K, Rajasuriar Hepatol 2011. regulate dendritic cell differentiation and function. R, Kramski M, Murray JM, Beard M et al. CD4+ J Immunol 2011; 187(8): 3911. Fear VS, Burchell JT, Lai SP, Wikstrom ME, Blank F, T-Cell Defi ciency in HIV Patients Responding von Garnier C, Turner DJ et al. Restricted Aeroallergen Lay CS, Ludlow LE, Stapleton D, Bellamy-McIntyre to Antiretroviral Therapy Is Associated With Access to Airway Mucosal Dendritic Cells In Vivo AK, Ramsland PA, Drummer HE, Poumbourios P. Role Increased Expression of Interferon-Stimulated Limits Allergen-Specifi c CD4+ T Cell Proliferation for the terminal clasp of HIV-1 gp41 in the initiation Genes in CD4+ T Cells. J Infect Dis 2011; during the Induction of Inhalation Tolerance. J of membrane fusion. J Biol Chem 2011. 204(12): 1927. Immunol 2011; 187(9): 4561. Woong Park S, Klotzsche M, Wilson DJ, Boshoff HI, Shklovskaya E, O’Sullivan BJ, Ng LG, Roediger Price P. Mycobacterial immune restoration disease: Eoh H, Manjunatha U, Blumenthal A et al. Evaluating B, Thomas R, Weninger W, Fazekas de St Groth B. one symphony, two or many? AIDS 2011. the sensitivity of Mycobacterium tuberculosis to Langerhans cells are precommitted to immune McSorley HJ, Gaze S, Daveson J, Jones D, Anderson biotin deprivation using regulated gene expression. tolerance induction. Proc Natl Acad Sci U S A RP, Clouston A, Ruyssers NE et al. Suppression of PLoS Pathog 2011; 7(9): e1002264. 2011; 108(44): 18049. infl ammatory immune responses in celiac disease Furuya Y, Chan J, Wan EC, Koskinen A, Diener KR, Vivian JP, Duncan RC, Berry R, O’Connor GM, by experimental hookworm infection. PLoS One Hayball JD, Regner M et al. Gamma-irradiated Reid HH, Beddoe T, Gras S et al. Killer cell 2011; 6(9): e24092. infl uenza virus uniquely induces IFN-I mediated immunoglobulin-like receptor 3DL1-mediated Wines BD, Trist HM, Farrugia W, Ngo C, Trowsdale lymphocyte activation independent of the TLR7/ recognition of human leukocyte antigen B. Nature J, Areschoug T, Lindahl G et al. A conserved host MyD88 pathway. PLoS One 2011; 6(10): e25765. 2011; 479(7373): 401. and pathogen recognition site on immunoglobulins: Stanley AC, de Labastida Rivera F, Haque A, Sheel Denton AE, Wesselingh R, Gras S, Guillonneau structural and functional aspects. Adv Exp Med Biol M, Zhou Y, Amante FH, Bunn PT et al. Critical roles C, Olson MR, Mintern JD, Zeng W et al. Affi nity 2012; 946: 87. for LIGHT and its receptors in generating T cell- Thresholds for Naive CD8+ CTL Activation by Brown GK, Kreiss A, Lyons AB, Woods GM. Natural mediated immunity during leishmania donovani Peptides and Engineered Infl uenza A Viruses. J killer cell mediated cytotoxic responses in the infection. PLoS Pathog 2011; 7(10): e1002279. Immunol 2011; 187(11): 5733. Tasmanian devil. PLoS One 2011; 6(9): e24475.

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27 ASI Inc. Newsletter December 2011

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