ACADSB Antibody / SBCAD (RQ5791)

Catalog No. Formulation Size

RQ5791 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug Bulk quote request

Availability 1-3 business days

Species Reactivity Human, Mouse, Rat, Monkey

Format Antigen affinity purified

Clonality Polyclonal (rabbit origin)

Isotype Rabbit IgG

Purity Affinity purified

Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

UniProt P45954

Applications Western blot : 0.5-1ug/ml Immunofluorescence : 2-4ug/ml Flow cytometry : 1-3ug/million cells

Limitations This ACADSB antibody is available for research use only.

Immunofluorescent staining of FFPE human MCF7 cells with ACADSB antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min. Flow cytometry testing of human ThP-1 cells with ACADSB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACADSB antibody.

Flow cytometry testing of mouse RAW264.7 cells with ACADSB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACADSB antibody.

Flow cytometry testing of rat RH-35 cells with ACADSB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACADSB antibody. Western blot testing of 1) human A431, 2) human T-47 D, 3) human A549, 4) monkey COS-7, 5) rat liver and 6) mouse liver lysate with ACADSB antibody. Expected molecular weight: ~47 kDa (unprocessed), ~43 kDa (processed).

Description ACADSB is a human gene that encodes short/branched chain specific acyl-CoA dehydrogenase (SBCAD), an enzyme in the acyl CoA dehydrogenase family. Short/branched chain acyl-CoA dehydrogenase (ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa.

Application Notes Optimal dilution of the ACADSB antibody should be determined by the researcher.

Immunogen Amino acids ASNIQLNTIAKHIDAEY from the human protein were used as the immunogen for the ACADSB antibody.

Storage After reconstitution, the ACADSB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.