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Arxula Adeninivorans
Biernacki et al. Microb Cell Fact (2017) 16:144 DOI 10.1186/s12934-017-0751-4 Microbial Cell Factories RESEARCH Open Access Enhancement of poly(3‑hydroxybutyrate‑co‑ 3‑hydroxyvalerate) accumulation in Arxula adeninivorans by stabilization of production Mateusz Biernacki1, Marek Marzec1,6, Thomas Roick2, Reinhard Pätz3, Kim Baronian4, Rüdiger Bode5 and Gotthard Kunze1* Abstract Background: In recent years the production of biobased biodegradable plastics has been of interest of research- ers partly due to the accumulation of non-biodegradable plastics in the environment and to the opportunity for new applications. Commonly investigated are the polyhydroxyalkanoates (PHAs) poly(hydroxybutyrate) and poly(hydroxybutyrate-co-hydroxyvalerate) (PHB-V). The latter has the advantage of being tougher and less brittle. The production of these polymers in bacteria is well established but production in yeast may have some advantages, e.g. the ability to use a broad spectrum of industrial by-products as a carbon sources. Results: In this study we increased the synthesis of PHB-V in the non-conventional yeast Arxula adeninivorans by stabilization of polymer accumulation via genetic modifcation and optimization of culture conditions. An A. adenini- vorans strain with overexpressed PHA pathway genes for β-ketothiolase, acetoacetyl-CoA reductase, PHAs synthase and the phasin gene was able to accumulate an unexpectedly high level of polymer. It was found that an opti- 1 mized strain cultivated in a shaking incubator is able to produce up to 52.1% of the DCW of PHB-V (10.8 g L− ) with 12.3%mol of PHV fraction. Although further optimization of cultivation conditions in a fed-batch bioreactor led to lower polymer content (15.3% of the DCW of PHB-V), the PHV fraction and total polymer level increased to 23.1%mol 1 and 11.6 g L− respectively. -
Phaff Collection News 2018 V1
Contents _________________ Phaff Collection p. 1 Honoring Robert and News Margrit Mondavi A novel yeast species was Fall 2018 named after generous UC Davis supporters New yeast species named after Robert and Margrit Mondavi pp. 2-3 In Fall 2008, the Phaff Yeast Culture Collection moved from a small, outdated space in Cruess Hall to spacious, new laboratory in the Robert Mondavi Institute. Ongoing research This building was named after winemaker Robert Mondavi, who with his wife New funding from USDA-NIFA Margrit donated $35 million to UC Davis in 2001 towards the building housing the Food Science & Technology and Viticulture & Enology departments, and the and the Almond Board of Robert and Margrit Mondavi Performing Arts Center. California This year, to honor the generosity of the Mondavi family, Phaff collection curator Kyria Boundy-Mills in collaboration with Gennadi Naumov and Elena Naumova named a yeast species after the Mondavis: Komagataella mondaviorum. Naming p. 4 a yeast species involves publishing a scientific paper in a peer-reviewed journal. Recent publications This publication was in a journal called Antonie van Leeuwenhoek, named after the inventor of the microscope. In the future, scientists studying this yeast can obtain it from the UC Davis Phaff Yeast Culture Collection, or the Westerdijk p. 5 Institute in the Netherlands, or the USDA-ARS collection in Peoria, Illinois. Participation in culture Some yeast species are named after a location, such as wine spoilage yeast Brettanomyces bruxellensis (named after Brussels, Belgium), or after the material collection organizations it came from such as Saccharomyces cerevisiae (meaning “from beer”). Some are US Culture Collection named after people, such as scientists who contributed to the field of yeast taxonomy Network or science in general, or benefactors such World Federation for Culture as supporters of habitat preservation. -
Yeasts of the Blastobotrys Genus Are Promising Platform for Lipid-Based
Yeasts of the Blastobotrys genus are promising platform for lipid-based fuels and oleochemicals production Daniel Sanya, Djamila Onésime, Volkmar Passoth, Mrinal Maiti, Atrayee Chattopadhyay, Mahesh Khot To cite this version: Daniel Sanya, Djamila Onésime, Volkmar Passoth, Mrinal Maiti, Atrayee Chattopadhyay, et al.. Yeasts of the Blastobotrys genus are promising platform for lipid-based fuels and oleochemicals production. Applied Microbiology and Biotechnology, Springer Verlag, 2021, 105, pp.4879 - 4897. 10.1007/s00253-021-11354-3. hal-03274326 HAL Id: hal-03274326 https://hal.inrae.fr/hal-03274326 Submitted on 30 Jun 2021 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Applied Microbiology and Biotechnology https://doi.org/10.1007/s00253-021-11354-3 MINI-REVIEW Yeasts of the Blastobotrys genus are promising platform for lipid-based fuels and oleochemicals production Daniel Ruben Akiola Sanya1 & Djamila Onésime1 & Volkmar Passoth2 & Mrinal K. Maiti3 & Atrayee Chattopadhyay3 & Mahesh B. Khot4 Received: 18 February 2021 /Revised: 29 April 2021 /Accepted: 16 May 2021 # The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 Abstract Strains of the yeast genus Blastobotrys (subphylum Saccharomycotina) represent a valuable biotechnological resource for basic biochemistry research, single-cell protein, and heterologous protein production processes. -
Investigation of Agonistic and Antagonistic Endocrine Activity During Full-Scale Ozonation of Waste Water
Investigation of agonistic and antagonistic endocrine activity during full-scale ozonation of waste water Dissertation zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften – Dr. rer. nat. – vorgelegt von Fabian Itzel geboren in Moers Fakultät für Chemie der Universität Duisburg-Essen 2018 Die vorliegende Arbeit wurde im Zeitraum von September 2014 bis September 2018 im Arbeitskreis von Prof. Dr. Torsten C. Schmidt in der Fakultät für Chemie im Bereich Instrumentelle Analytische Chemie der Universität Duisburg-Essen durchgeführt. Tag der Disputation: 21.02.2019 Gutachter: Prof. Dr. Torsten C. Schmidt Prof. Dr. Elke Dopp Vorsitzender: Prof. Dr. Bettina Siebers I Summary The use of a wide variety of chemicals in our society, such as industrial chemicals, pharmaceuticals, personal care products, etc., leads to pollution of surface waters. Especially in densely populated urban areas such as the Ruhr catchment, sustainable water management poses a major challenge. Despite intensive use through various types of discharges (effluents of direct dischargers, municipal waste water treatment plants, industry, etc.), good water quality has always to be guaranteed in accordance to the European Water Framewor Directive. Endocrine disrupting chemicals can have an effect on aquatic organisms even at very low concentrations (pg/L range). In order to reduce the emission, ozonation was investigated as advanced waste water treatment for the elimination of organic trace compounds. An elimination performance of ≥ 80% for selected substances at specific ozone doses in the range of zspec. = 0.3 - 0.7 mgO3/mgDOC was achieved. Since 2015, estrogens are listed on the watch-list of the European Water Framework Directive with required detection limits in the pg/L range. -
The Microbial Ecology of Wine Grape Berries
International Journal of Food Microbiology 153 (2012) 243–259 Contents lists available at SciVerse ScienceDirect International Journal of Food Microbiology journal homepage: www.elsevier.com/locate/ijfoodmicro Review The microbial ecology of wine grape berries A. Barata ⁎, M. Malfeito-Ferreira, V. Loureiro Laboratório de Microbiologia, Centro de Botânica Aplicada à Agricultura, Technical University of Lisbon, 1349-017 Lisbon, Portugal article info abstract Article history: Grapes have a complex microbial ecology including filamentous fungi, yeasts and bacteria with different Received 8 July 2011 physiological characteristics and effects upon wine production. Some species are only found in grapes, Received in revised form 28 October 2011 such as parasitic fungi and environmental bacteria, while others have the ability to survive and grow in Accepted 27 November 2011 wines, constituting the wine microbial consortium. This consortium covers yeast species, lactic acid bacteria Available online 2 December 2011 and acetic acid bacteria. The proportion of these microorganisms depends on the grape ripening stage and on the availability of nutrients. Grape berries are susceptible to fungal parasites until véraison after which the Keywords: Microbial ecology microbiota of truly intact berries is similar to that of plant leaves, which is dominated by basidiomycetous Grapes yeasts (e.g. Cryptococcus spp., Rhodotorula spp. Sporobolomyces spp.) and the yeast-like fungus Aureobasidium Damaged grapes pullulans. The cuticle of visually intact berries may bear microfissures and softens with ripening, increasing Spoilage yeasts nutrient availability and explaining the possible dominance by the oxidative or weakly fermentative ascomy- Acetic acid bacteria cetous populations (e.g. Candida spp., Hanseniaspora spp., Metschnikowia spp., Pichia spp.) approaching har- Lactic acid bacteria vest time. -
Development of an Enzyme-Based Method for Production of Food with Low Purine Content
Development of an enzyme-based method for production of food with low purine content I n a u g u r a l d i s s e r t a t i o n zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.) der Mathematisch-Naturwissenschaftlichen Fakultät der Ernst-Moritz-Arndt-Universität Greifswald vorgelegt von Dagmara Jankowska geboren am 27. Juni 1984 in Wrocław, Polen - Greifswald, 2013 - Dekan: Prof. Dr. Klaus Fesser 1. Gutachter : Prof. Dr. habil. Rϋdiger Bode Universität Greifswald, Institut für Biochemie 2. Gutachter: Prof. Dr. Raffael Schaffrath Universität Kassel, Institut für Biologie Tag der Promotion: 14.04.2014 Table of contents TABLE OF CONTENTS Summary ............................................................................................................................ i Zusammenfassung ............................................................................................................ iii List of abbreviations .......................................................................................................... v 1 Introduction .................................................................................................................. 1 1.1 Purine degradation pathway ........................................................................................ 1 1.1.1 Purines .............................................................................................................. 1 1.1.2 Purine degradation .......................................................................................... -
William B. Heed 1926–2007
IN MEMORIAM doi:10.1111/j.1558-5646.2007.00269.x WILLIAM B. HEED 1926–2007 William Battles Heed (b. June 9, 1926) died September 10, 2007 after a long illness, leaving his spouse of 53 years, Sarah, and three daughters, Ellen, Emily, and Anne. Perhaps best known for his long-term contributions to the ecology, genetics, distribution, and host plant relationships of desert Drosophila, Bill Heed was also a consummate field naturalist who came from a long tradition of “knowing” the organisms he worked with. A biography of Heed’s life was recorded as a dedication to the second symposium volume concerning ecology and genetics of Drosophila (Fogleman 1990), which included input from him and a complete bibliography, so much of that account will not be repeated. A perspective on Bill’s unique contributions to ecological genetics and evolution early in his career, some relevant references, his pursuit of truth, as well as his insights into science and his colleagues throughout his life, are described here. Bill Heed was an avid field biologist from a very young age, and he exercised a passion for field ornithology for the rest of life. After graduating from high school, he joined the Navy in 1944 and served in the South Pacific. He obtained his B.S. degree in 1950 from Penn State University, and after some illumination from reading Dobzhansky’s Genetics and the Origin of Species (1951), began graduate school at the University of Texas at Austin, becoming John T. Patterson’s last graduate student. Along with Wilson S. Stone, Marshall Wheeler, B. -
ISSN 0513-5222 Official Publication of the International Commission On
ISSN 0513-5222 Official Publication of the International Commission on Yeasts of the International Union of Microbiological Societies (IUMS) JUNE 2004 Volume LIII, Number I Marc-André Lachance, Editor University of Western Ontario, London, Ontario, Canada N6A 5B7 <[email protected]> Associate Editors Peter Biely G.G. Stewart Institute of Chemistry International Centre for Brewing and Distilling Slovak Academy of Sciences Department of Biological Sciences Dúbravská cesta 9 Heriot-Watt University 842 38 Bratislava, Slovakia Riccarton, Edinburgh EH14 4AS, Scotland Yasuji Oshima Patrizia Romano Department of Biotechnology Dipartimento di Biologia, Difesa Faculty of Engineering e Biotecnologie Agro-Forestali Kansai University Università della Basilicata, 3-3-35 Yamate-Cho, Suita-Shi Via Nazario Sauro, 85, Osaka 564-8680, Japan 85100 Potenza, Italy W.I. Golubev, Puschino, Russia ............ 1 W.M. Ingledew, Saskatoon, J.A. Barnett, Norwich, England ............. 1 Saskatchewan, Canada ................8 A. Caridi, Gallina, Italy ................... 2 G. Kunze, Gaterleben, Germany ..............9 L. Olsson, Lyngby, Denmark ............... 2 M.A. Lachance, London, Ontario, Canada .....10 E. Minárik, Bratislava, Slovakia ............ 4 Obituary ................................11 G.I. Naumov and E.S. Naumova, International Commission on Yeasts ..........11 Moscow, Russia .................. 4 Recent event .............................11 Á. Fonseca and J.P. Sampaio, Caparica, Forthcoming meetings .....................12 Portugal ......................... 5 Brief News Items .........................12 A. Speers, Halifax, Nova Scotia, Canada ..... 6 Publication of Interest .....................13 J. du Preez, Bloemfontein, South Africa ...... 7 Editorials International surface post rate no longer available Due to a recent review of rates by Canada Post, the preferential prices for "international surface" and “printed papers” will no longer be available. A uniform subscription rate of USD$12.00 will therefore be implemented for all mailings outside Canada or the USA. -
Arxula Adeninivorans Recombinant Guanine Deaminase and Its Application in the Production of Food with Low Purine Content
Research Article J Mol Microbiol Biotechnol 2014;24:67–81 Received: June 25, 2013 DOI: 10.1159/000357674 Accepted: November 29, 2013 Published online: January 24, 2014 Arxula adeninivorans Recombinant Guanine Deaminase and Its Application in the Production of Food with Low Purine Content a a b Anke Trautwein-Schult Dagmara Jankowska Arno Cordes b b a a Petra Hoferichter Christina Klein Andrea Matros Hans-Peter Mock d c a Keith Baronian Rüdiger Bode Gotthard Kunze a b Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben , ASA Spezialenzyme GmbH, c d Wolfenbüttel , and Institute of Microbiology, University of Greifswald, Greifswald , Germany; School of Biological Sciences, University of Canterbury, Christchurch , New Zealand Key Words ole of yeast. The enzyme was demonstrated to reduce levels Arxula adeninivorans · Hyperuricemia · Recombinant of guanine in food. A mixture of guanine deaminase and oth- guanine deaminase · Treatment of food er purine degradation enzymes will allow the reduction of purines in purine-rich foods. © 2014 S. Karger AG, Basel Abstract Purines of exogenous and endogenous sources are degrad- ed to uric acid in human beings. Concentrations >6.8 mg uric Introduction acid/dl serum cause hyperuricemia and its symptoms. Phar- maceuticals and the reduction of the intake of purine-rich Humans absorb different amounts of RNA, DNA, nu- food are used to control uric acid levels. A novel approach to cleotides, nucleosides and free purine bases from food of the latter proposition is the enzymatic reduction of the pu- animal and vegetable origin [Wolfram and Colling, 1987]. rine content of food by purine-degrading enzymes. -
Centraalbureau Voor Schimmelcultures: Hundred Years Microbial Resource Centre
STUDIES IN MYCOLOGY 50: 1–8. 2004. Centraalbureau voor Schimmelcultures: hundred years microbial resource centre Robert A. Samson*, Huub A. van der Aa and G. Sybren de Hoog Centraalbureau voor Schimmelcultures, P.O. Box 85167, 3508 AD Utrecht, The Netherlands *Correspondence: Robert A. Samson, [email protected]. Abstract: The Centraalbureau voor Schimmelcultures was established in 1904 by the Association Internationale des Botanistes. In 2004, it celebrated its 100 year anniversary. The collection was founded by F.A.F.C. Went and started with the maintenance of 78 cultures, most of which were collected in Indonesia. Presently CBS is one of the largest mycological centres in the world, with holdings of over 60 000 cultures, including many ex-type isolates of filamentous fungal and yeast species. The institute and its holdings have been at the basis of most major scientific breakthroughs in mycology. Pure culture: a scientific revolution being appointed at the Sugar Cane Experimental At the end of 19th century, cultivation of microorgan- Station (Peirce 1936). Besides phytopathogens, he isms was no less than a scientific revolution. Without also isolated many yeast and filamentous fungal pure cultures, Louis Pasteur (1822–1895) would not strains that he recognized as playing an essential role have been able to carry out the experiments that had in the production of Asian fermented foods. such a profound impact on today’s medical practice. The necessity of an international depository for Robert Koch (1843–1910) laid the foundation of a fungal cultures was discussed in 1903 during a meet- new era in phytopathology by using cultures to realize ing of the board of the Association Internationale des his classic postulates for distinguishing phytopatho- Botanistes in Leiden. -
Mating-Type Switching by Chromosomal Inversion in Methylotrophic Yeasts Suggests an Origin for the Three-Locus Saccharomyces
Mating-type switching by chromosomal inversion PNAS PLUS in methylotrophic yeasts suggests an origin for the three-locus Saccharomyces cerevisiae system Sara J. Hanson, Kevin P. Byrne, and Kenneth H. Wolfe1 UCD Conway Institute, School of Medicine and Medical Science, University College Dublin, Dublin 4, Ireland Edited by Jasper Rine, University of California, Berkeley, CA, and approved October 7, 2014 (received for review August 19, 2014) Saccharomyces cerevisiae has a complex system for switching the S. cerevisiae (subphylum Saccharomycotina) and Schizosaccharomyces mating type of haploid cells, requiring the genome to have three pombe (Taphrinomycotina). A large clade of nonswitching fil- mating-type (MAT)–like loci and a mechanism for silencing two of amentous ascomycetes, the Pezizomycotina, is related more closely them. How this system originated is unknown, because the three- to S. cerevisiae than to S. pombe (Fig. 1). Despite their apparently locus system is present throughout the family Saccharomyceta- independent origins, the switching systems of S. cerevisiae (3) and ceae, whereas species in the sister Candida clade have only one S. pombe (4, 5) have many parallels, including the presence of three locus and do not switch. Here we show that yeasts in a third clade, MAT-like loci (two of which are silenced) with triplicated guide the methylotrophs, have a simpler two-locus switching system sequences, a mechanism for specifically inducing a DNA break at based on reversible inversion of a section of chromosome with MAT, donor bias (27), and cell-lineage tracking. However, the MATa genes at one end and MATalpha genes at the other end. details of switching and silencing are quite different in the two In Hansenula polymorpha the 19-kb invertible region lies beside species (3, 4). -
Evaluation of Ogataea (Hansenula) Polymorpha for Hyaluronic Acid Production
microorganisms Article Evaluation of Ogataea (Hansenula) polymorpha for Hyaluronic Acid Production João Heitor Colombelli Manfrão-Netto 1 , Enzo Bento Queiroz 1, Kelly Assis Rodrigues 1, Cintia M. Coelho 2 , Hugo Costa Paes 3 , Elibio Leopoldo Rech 4 and Nádia Skorupa Parachin 1,5,* 1 Grupo Engenharia de Biocatalisadores, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília 70910-900, Brazil; [email protected] (J.H.C.M.-N.); [email protected] (E.B.Q.); [email protected] (K.A.R.) 2 Department of Genetics and Morphology, Institute of Biological Science, University of Brasília, Brasília 70910-900, Brazil; [email protected] 3 Clinical Medicine Division, University of Brasília Medical School, University of Brasília, Brasília 70910-900, Brazil; [email protected] 4 Brazilian Agriculture Research Corporation—Embrapa—Genetic Resources and Biotechnology—CENARGEN, Brasília 70770-917, Brazil; [email protected] 5 Ginkgo Bioworks, Boston, MA 02210, USA * Correspondence: [email protected] Abstract: Hyaluronic acid (HA) is a biopolymer formed by UDP-glucuronic acid and UDP-N-acetyl- glucosamine disaccharide units linked by β-1,4 and β-1,3 glycosidic bonds. It is widely employed in medical and cosmetic procedures. HA is synthesized by hyaluronan synthase (HAS), which catalyzes the precursors’ ligation in the cytosol, elongates the polymer chain, and exports it to the extracellular space. Here, we engineer Ogataea (Hansenula) polymorpha for HA production by inserting the genes encoding UDP-glucose 6-dehydrogenase, for UDP-glucuronic acid production, and HAS. Two microbial HAS, from Streptococcus zooepidemicus (hasAs) and Pasteurella multocida Citation: Manfrão-Netto, J.H.C.; (hasAp), were evaluated separately.