/Mouse Polypeptide GalNac 10/GALNT10 Antibody Antigen Affinity-purified Polyclonal Sheep IgG Catalog Number: AF7575

DESCRIPTION Species Reactivity Human/Mouse

Specificity Detects human and mouse Polypeptide GalNac Transferase 10/GALNT10 in Western blots. In direct ELISA, less than 1% cross­reactivity with recombinant human (rh) GALNT1 and rhGALNT4 is observed. Source Polyclonal Sheep IgG

Purification Antigen Affinity­purified

Immunogen Mouse myeloma cell line NS0­derived recombinant human Polypeptide GalNac Transferase 10/GALNT10 Leu71­Asn603 Accession # Q86SR1 Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (­SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.

APPLICATIONS Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website. Recommended Sample Concentration Western Blot µg/mL No Sample Info Immunohistochemistry 5­15 µg/mL See Below

DATA

Immunohistochemistry Polypeptide GalNac Transferase 10/ GALNT10 in Mouse Testis. Polypeptide GalNac Transferase 10/GALNT10 was detected in perfusion fixed frozen sections of mouse testis using Sheep Anti­Human/ Mouse Polypeptide GalNac Transferase 10/ GALNT10 Antigen Affinity­purified Polyclonal Antibody (Catalog # AF7575) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti­Sheep HRP­DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Golgi organelles in spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin­embedded Tissue Sections.

PREPARATION AND STORAGE Reconstitution Sterile PBS to a final concentration of 0.2 mg/mL. Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (­SP) is shipped with polar packs. Upon receipt, store it immediately at ­20 to ­70 °C Stability & Storage Use a manual defrost freezer and avoid repeated freeze­thaw cycles. l 12 months from date of receipt, ­20 to ­70 °C as supplied. l 1 month, 2 to 8 °C under sterile conditions after reconstitution. l 6 months, ­20 to ­70 °C under sterile conditions after reconstitution.

BACKGROUND GALNT10 (N­Acetyl­Galactosaminyl Transferase 10; also UDP­Acetylgalactosaminyltransferase 10 and ppGalNAc­T10) is a member of the GalNAC transferase subfamily, glycosyltransferase 2 family of . It is widely expressed, being found in intestine, pancreas, thyroid and spleen. GALNT10 is found in the Golgi apparatus, and catalyzes the transfer of UDP­GalNAc onto either a Ser or Thr residue on a previously glycosylated /polypeptide backbone. Human GALNT2 is a 603 amino acid (aa) type II transmembrane glycoprotein. It contains an 11 aa N­terminal cytoplasmic region and a 572 aa extracellular domain (aa 32­603). The ECD possesses two key parts, a catalytic region with two catalytic subdomains (aa 144­253 and 311­373), and a B­type domain that binds carbohydrates (aa 458­590). These two distinct domains have unique but complimentary properties. By itself, an active catalytic domain can do no more than place a GalNAc residue immediately adjacent to an existing C­terminal GalNAC residue. Alternatively, if the lectin domain is involved, this domain may recognize (bind to) any number of existing sites, allowing for the subsequent attachment of GalNAc by the catalytic site onto a Ser or Thr residue quite distant from the initial lectin domain:CHO recognition site. There are at least three potential splice form variants. One shows a deletion of aa 190­251, a second contains a 13 aa substitution for aa 354­366, and a third possesses a 23 aa substitution for aa 1­352 coupled to a three aa substitution for Asn389. Over aa 71­603, human GALNT10 shares 96% aa sequence identity with mouse GALNT10.

Rev. 2/6/2018 Page 1 of 1