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Catalogue# MCA-2C4: Mouse Monoclonal to MAP2C/D

The Immunogen: Microtubules are 25nm diameter rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c and MAP2d, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (a.k.a. MAP-tau or tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. to MAP2 are therefore excellent markers of neuronal cells, their perikarya and neuronal dendrites. MCA-2C4 was raised against recombinant human MAP2D and also binds to recombinant human MAP2C. The antibody epitope is therefore in the core of the MAP2 molecule and so this antibody will bind all known MAP2 isoforms as shown in the blot. MAP2C and MAP2D are expressed earlier in development than the MAP2a and MAP2b isoforms, so that this antibody is a more useful marker of neuronal development. In contrast MAP-τ (MAP-tau) is found predominantly but not exclusively in neuronal axons. The HGNC name for this protein is MAP2.

Figures: Left: Blots of brain extract from day18 embryonic rat were probed with MCA-2C4 at 1:10,000 (lane 1) and 1:5,000 (lane 2). The strong bands running at about 280 kDa corresponds to MAP2A/2B. Bands running at 70kDa correspond to MAP2C/D. Right: Mixed rat neuron and glia cultures stained with MCA-2C4 (red) and chicken antibody to (green) and DNA (blue). MCA-2C4 antibody reveals strong cytoplasmic staining of dendrites and perikarya of neuronal cells, while vimentin was visualized in astrocytes and fibroblasts.

Antibody characteristics: MCA-2C4 was raised against recombinant human MAP2D purified from E. coli and is supplied as an aliquot of purified antibody at 1 mg/mL in 50%glycerol/PBS with 5 mM sodium azide as a preservative. MCA-2C4 is a mouse IgG1 class antibody. MCA-2C4 recognizes human and rodent all MAP2 isoforms including MAP2A/B and MAP2C/D both in western blots and in immunocytochemical experiments.

Suggestions for use: Try at dilutions of 1:5,000-1:10,000 for immunofluorescence and Western blots.

Omim link: http://omim.org/entry/157130

References: 1. Dehmelt L, Halpain S. The MAP2/Tau family of microtubule-associated proteins. Genome Biol. 6:204 (2004)

2. Vallee R. A taxol-dependent procedure for the isolation of microtubules and microtubule-associated proteins

(MAPs). J. Cell Biol. 92:435-442 (1992)

Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis.

©EnCor Inc. October 14, 2015.