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AR09348PU-N Human PDIA3 (25-505, His-tag) - Purified

Alternate names: isomerase ER-60, ER marker, ERP57, ERP60, GRP58, Disulfide Isomerase, Protein disulfide-isomerase A3 Quantity: 0.1 mg Concentration: 1.0 mg/ml (determined by Bradford assay) Background: PDIA3, also known as protein disulfide-isomerase A3, is a protein of the that interacts with lectin chaperones and to modulate folding of newly synthesized . This protein has protein disulfide isomerase activity. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is essential for formation of the final conformation and export from the endoplasmic reticulum to the cell surface. Uniprot ID: P30101 NCBI: NP_005304 GeneID: 2923 Species: Human Source: E. coli Format: State: Liquid purified protein Purity: >95% by SDS - PAGE Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 0.1 M NaCl, and 10% glycerol Description: Recombinant Human PDIA3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSDV LELTDDNFES RISDTGSAGL MLVEFFAPWC GHCKRLAPEY EAAATRLKGI VPLAKVDCTA NTNTCNKYGV SGYPTLKIFR DGEEAGAYDG PRTADGIVSH LKKQAGPASV PLRTEEEFKK FISDKDASIV GFFDDSFSEA HSEFLKAASN LRDNYRFAHT NVESLVNEYD DNGEGIILFR PSHLTNKFED KTVAYTEQKM TSGKIKKFIQ ENIFGICPHM TEDNKDLIQG KDLLIAYYDV DYEKNAKGSN YWRNRVMMVA KKFLDAGHKL NFAVASRKTF SHELSDFGLE STAGEIPVVA IRTAKGEKFV MQEEFSRDGK ALERFLQDYF DGNLKRYLKS EPIPESNDGP VKVVVAENFD EIVNNENKDV LIEFYAPWCG HCKNLEPKYK ELGEKLSKDP NIVIAKMDAT ANDVPSPYEV RGFPTIYFSP ANKKLNPKKY EGGRELSDFI SYLQREATNP PVIQEEKPKK KKKAQEDL Molecular weight: 58.5 kDa (518 aa) confirmed by MALDI-TOF Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Vigneron N, Peaper DR, Leonhardt RM, Cresswell P. Functional significance of membrane association and disulfide linkage to ERp57 in MHC class I presentation. Eur J Immunol. 2009 Sep;39(9):2371-6. doi: 10.1002/eji.200939536. PubMed PMID: 19701894.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20150119 1 / 2 AR09348PU-N: Human PDIA3 (25-505, His-tag) - Purified

2. Forster ML, Mahn JJ, Tsai B. Generating an unfoldase from thioredoxin-like domains. J Biol Chem. 2009 May 8;284(19):13045-56. doi: 10.1074/jbc.M808352200. Epub 2009 Mar 16. PubMed PMID: 19289469. Pictures: Recombinant human PDIA3, 25-505 aa, His-tagged: 15% SDS-PAGE (3 µg)

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20150119 2 / 2

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