Respiratory Virus Panel by PCR

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Respiratory Virus Panel by PCR Excellence in Pathology and Laboratory Services New Assay: Respiratory Virus Panel by PCR Overview October, 2009 PathGroup Labs is pleased to announce the infection will aid in the diagnosis and treatment immediate availability the FDA-cleared of respiratory viral infection. Luminex Respiratory Virus Panel by PCR Influenza A and Novel H1N1 (swine) Influenza assay. This assay detects the following 10 A: This panel will detect all known Influenza A different common respiratory viruses from a viruses and subtype the common seasonal H1 single nasopharyngeal specimen: Influenza A and H3 viruses. It is important to note that this (subtype H1), Influenza A (subtype H3), assay does not directly subtype the novel H1N1 Influenza A (other subtype), Influenza B, (swine) Influenza A virus that has been in Respiratory Syncytial Virus subtype A, circulation since Spring, 2009. A specimen that Respiratory Syncytial Virus subtype B, is positive for the novel H1N1 (swine) Influenza Parainfluenza 1, Parainfluenza 2, and A will have a result of Influenza A (other Parainfluenza 3 virus, Human subtype) Detected. Current recommendations Metapneumovirus, Rhinovirus, and from the CDC and most state health Adenovirus. This assay will be performed 5 departments are that specific identification of days a week, Tuesday through Saturday, so that the novel H1N1 (swine) Influenza A is not a 24-48 hour turn-around time is achieved. This necessary to begin treatment; the identification quick detection and identification of specific of Influenza A is necessary and sufficient for viral nucleic acids from individuals presenting treatment. with signs and symptoms of respiratory Clinical Utility Detection and identification of common respiratory viruses Methodology: Reverse-transcription Polymerase Chain Reaction and xMAP Detection Test Codes: RVP CPT Codes: 87798 (10 units) Specimen Collection: Collect a nasopharyngeal specimen using the supplied flocked swabs and M4 media. Place the swab in the M4 container and break the swab shaft at the scored mark. Leave the swab head in the media. Do not use wooden shaft swabs or cotton tip swabs. Dacron head nylon shaft swabs are acceptable but not preferred. Store specimens frozen. Informative videos on collecting nasopharyngeal specimens can be found at http://copanusa.com/videos/education/index.php Shipping and Handling: Transport specimens frozen. Associated Pathologists, PLC and PathGroup Labs 658 Grassmere Park Suite 101 Nashville TN 37211 615-562-9300 888-474-5227 Excellence in Pathology and Laboratory Services Reference Ranges: The reference range for each virus is Not Detected. Turnaround Time: 1-2 days References 1. Anzueto, A. and M. S. Niederman (2003). 13. Monto, A. S. (1994). "Studies of the community "Diagnosis and treatment of rhinovirus and family: acute respiratory illness and respiratory infections." Chest 123(5): 1664-72. infection." Epidemiol Rev 16(2): 351-73. 2. Arruda, E., A. Pitkaranta, et al. (1997). 14. Mullins, J. A., D. D. Erdman, et al. (2004). "Frequency and natural history of rhinovirus "Human metapneumovirus infection among infections in adults during autumn." J Clin children hospitalized with acute respiratory Microbiol 35(11): 2864-8. illness." Emerg Infect Dis 10(4): 700-5. 3. Brandt, C. D., H. W. Kim, et al. (1969). 15. Pitkaranta, A. and F. G. Hayden (1998). "Infections in 18,000 infants and children in a "Rhinoviruses: important respiratory pathogens." controlled study of respiratory tract disease. I. Ann Med 30(6): 529-37. Adenovirus pathogenicity in relation to serologic 16. Rota, P. A., M. S. Oberste, et al. (2003). type and illness syndrome." Am J Epidemiol "Characterization of a novel coronavirus 90(6): 484-500. associated with severe acute respiratory 4. Chidgey, S. M. and K. J. Broadley (2005). syndrome." Science 300(5624): 1394-9. "Respiratory syncytial virus infections: 17. Rotbart, H. A. and F. G. Hayden (2000). characteristics and treatment." J Pharm "Picornavirus infections: a primer for the Pharmacol 57(11): 1371-81. practitioner." Arch Fam Med 9(9): 913-20. 5. Edwards, K. M., J. Thompson, et al. (1985). 18. Ryan M.a., Gray G.C., et al. (2002). “Large "Adenovirus infections in young children." epidemic of respiratory illness due to adenovirus Pediatrics 76(3): 420-4. types 7 and 3 in healthy young adults.” Clin 6. Hayden, F. G. and M. G. Ison (2005). Respiratory Infect Dis. 34(5): 577-82. Viruses. ACP Medicine. 2006. 19. Stalkup, J. R. and S. Chilukuri (2002). 7. Heirholzer J.C. (1992). “Adenoviruses in the "Enterovirus infections: a review of clinical immunocompromised host.” Clin Microbiol Rev. presentation, diagnosis, and treatment." Dermatol 5(3): 262-74. Clin 20(2): 217-23. 8. Kuiken, T., R. A. Fouchier, et al. (2003). "Newly 20. Turner, R. B. (1998). "The common cold." discovered coronavirus as the primary cause of Pediatr Ann 27(12): 790-5. severe acute respiratory syndrome." Lancet 21. van den Hoogen, B. G., J. C. de Jong, et al. 362(9380): 263-70. (2001). "A newly discovered human 9. Makela, M. J., T. Puhakka, et al. (1998). "Viruses pneumovirus isolated from young children with and bacteria in the etiology of the common cold." respiratory tract disease." Nat Med 7(6): 719-24. J Clin Microbiol 36(2): 539-42. 22. van der Hoek, L., K. Pyrc, et al. (2004). 10. MMWR (2007). “Acute respiratory disease "Identification of a new human coronavirus." Nat associated with adenovirus serotype 14 – Four Med 10(4): 368-73. States, 2006-2007.” 56(45); 1181-1184. 23. Williams, J. V., P. A. Harris, et al. (2004). 11. MMWR (2007). “Update – Influenza Activity – "Human metapneumovirus and lower respiratory United States and Worldwide, 2006-07 flu tract disease in otherwise healthy infants and season, and composition of the 2007-08 Influenza children." N Engl J Med 350(5): 443-50. vaccine”. 56(31):789-794. 24. Yarush, L. I. and R. W. Steele (2000). "Diagnosis 12. MMWR (2006). “Brief report – Respiratory and prospective treatment of enteroviral Syncytial virus activity – United States 2005-06”. infections in children." Clin Pediatr (Phila) 39(4): 55(47):1277-1279. 209-11. Associated Pathologists, PLC and PathGroup Labs 658 Grassmere Park Suite 101 Nashville TN 37211 615-562-9300 888-474-5227 .
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