EIF2S1 Antibody Cat. No.: 57-063

EIF2S1 Antibody

Confocal immunofluorescent analysis of EIF2S1 Antibody EIF2S1 Antibody immunohistochemistry analysis in with Hela cell followed by Alexa Fluor 488-conjugated goat formalin fixed and paraffin embedded human stomach anti-rabbit lgG (green). DAPI was used to stain the cell tissue followed by peroxidase conjugation of the nuclear (blue). secondary antibody and DAB staining.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Mouse

Predicted species reactivity based on immunogen sequence: Rat, Chicken, Yeast, Bovine, HOMOLOGY: Drosophila

This EIF2S1 antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 60-89 amino acids from the N-terminal region of human EIF2S1.

TESTED APPLICATIONS: IF, IHC-P, WB

September 30, 2021 1 https://www.prosci-inc.com/eif2s1-antibody-57-063.html For WB starting dilution is: 1:1000

APPLICATIONS: For IF starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50

PREDICTED MOLECULAR 36 kDa WEIGHT:

Properties

This antibody is purified through a A column, followed by peptide affinity PURIFICATION: purification.

CLONALITY: Polyclonal

ISOTYPE: Rabbit Ig

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.

CONCENTRATION: batch dependent

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: EIF2S1

Eukaryotic 2 subunit 1, initiation factor 2 ALTERNATE NAMES: subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2S1, EIF2A

ACCESSION NO.: P05198

PROTEIN GI NO.: 124200

GENE ID: 1965

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

September 30, 2021 2 https://www.prosci-inc.com/eif2s1-antibody-57-063.html The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 BACKGROUND: nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the state of EIF2- alpha (Ernst et al., 1987 [PubMed 2948954]).

REFERENCES: 1) Shiota, M., et al. Int. J. Oncol. 36(6):1521-1531(2010)

2) Kulkarni, A.P., et al. Indian J. Biochem. Biophys. 47(2):67-74(2010)

3) Wehner, K.A., et al. Mol. Cell. Biol. 30(8):2006-2016(2010)

4) Backes, S., et al. J. Gen. Virol. 91 (PT 2), 470-482 (2010) :

ANTIBODIES FOR RESEARCH USE ONLY.

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