77 (3): 433 – 455 2019 © Senckenberg Gesellschaft für Naturforschung, 2019. The phylogeny of Ptiliidae (Coleoptera: Staphylinoidea) – the smallest beetles and their evolutionary transformations ,1, 2 3 4 Alexey A. Polilov* , Ignacio Ribera , Margarita I. Yavorskaya , Anabela Cardoso 3, Vasily V. Grebennikov 5 & Rolf G. Beutel 4 1 Department of Entomology, Biological Faculty, Lomonosov Moscow State University, Moscow, Russia; Alexey A. Polilov * [polilov@gmail. com] — 2 Joint Russian-Vietnamese Tropical Research and Technological Center, Hanoi, Vietnam — 3 Institute of Evolutionary Biology (CSIC-Universitat Pompeu Fabra), Barcelona, Spain; Ignacio Ribera [
[email protected]]; Anabela Cardoso [
[email protected]] — 4 Institut für Zoologie und Evolutionsforschung, FSU Jena, Jena, Germany; Margarita I. Yavorskaya [
[email protected]]; Rolf G. Beutel [
[email protected]] — 5 Canadian Food Inspection Agency, Ottawa, Canada; Vasily V. Grebennikov [
[email protected]] — * Cor- responding author Accepted on November 13, 2019. Published online at www.senckenberg.de/arthropod-systematics on December 06, 2019. Published in print on December 20, 2019. Editors in charge: Martin Fikáček & Klaus-Dieter Klass. Abstract. The smallest beetles and the smallest non-parasitic insects belong to the staphylinoid family Ptiliidae. Their adult body length can be as small as 0.325 mm and is generally smaller than 1 mm. Here we address the phylogenetic relationships within the family using formal analyses of adult morphological characters and molecular data, and also a combination of both for the frst time. Strongly supported clades are Ptiliidae + Hydraenidae, Ptiliidae, Ptiliidae excl. Nossidium, Motschulskium and Sindosium, Nanosellini, and a clade comprising Acrotrichis, Smicrus, Nephanes and Baeocrara. A group comprising Actidium, Oligella and Micridium + Ptilium is also likely monophy- letic.