Rabbit Anti-human , gamma 1 (TUBG1) Monoclonal Antibody, Clone 2D2 YUROGEN BIOSYSTEMS Catalog Number: R17033M2D2 Antibody Expert You Can Trust

General Information Preparation Monoclonal antibody is produced by immunizing rabbit with Truncated recombinant human Immunogen truncated human TUBG1 and purified using protein TUBG1 protein A resin. IgG type Rabbit IgG Application Clonality Monoclonal Western blot 1 2 Cell lysate: Applications ELISA, WB, IF (1:200 to 1:2000) lane 1: MDA231, scramble RNA, 25 ug; lane 2: MDA231, shRNA against human BRG1, 25 ug Specificity Human TUBG1 170 KD - 135 KD - 1xPBS, pH 7.4, 2D2 dilution: 1ug/ml Formulation 0.09% sodium azide 95 KD - Blocking and antibody dilution > 95% determined by SDS-PAGE 72 KD - buffer is 5% skim milk (w/v), 1x Purity TBS, 0.05% Tween-20. o Storage ≤ -20 C for 1 year or 4 oC for 1 months Abbreviations: ELISA: Enzyme-linked immunosorbent assay; ITA: immunoturbidimet- ric assay; IP: immunoprecipitation; IHC: immuno-histochemistry; IF: immunofluorescence. WB: western blot; FC: flow cytometry Immunofluorescence Storage This antibody is shipped at 4 oC. This product is stable for 12 months from date of receipt when stored at -20 oC to -70 oC. Avoid freeze/thaw cycles. Hazard/Biohazard This antibody contains 0.09% sodium azide as preserva- tive. Please handle and dispose the product properly. No known biohazard is associated with this product. Background Tubulin, gamma 1（TUBG1）is a member of tubulin superfamily that mainly localizes to organiz- ing centers (MOTC), centrioles (parts of ) and spindle poles. TUBG1 is the essential component of gamma tubulin ring complex that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation. Disruption of gamma Tubulin leads to mitotic arrest and decrease in microtubule quantity. Diseases associated with TUBG1 include Complex Corti- cal Dysplasia with Other Brain Malformations 4 and Pachygyria.

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