VARS Rabbit Pab
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Leader in Biomolecular Solutions for Life Science VARS Rabbit pAb Catalog No.: A4182 1 Publications Basic Information Background Catalog No. Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate A4182 amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first Observed MW proteins that appeared in evolution. The protein encoded by this gene belongs to class-I 140kDa aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. Calculated MW 33kDa/140kDa Category Primary antibody Applications WB, IHC, IF Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 7407 P26640 IHC 1:50 - 1:100 Immunogen 1:50 - 1:100 IF Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human VARS (NP_006286.1). Synonyms VARS;G7A;VARS1;VARS2 Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using VARS antibody (A4182) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- embedded rat brain using VARS antibody embedded human breast cancer using embedded mouse brain using VARS (A4182) at dilution of 1:100 (40x lens). VARS antibody (A4182) at dilution of 1:100 antibody (A4182) at dilution of 1:100 (40x (40x lens). lens). Immunofluorescence analysis of L929 cells using VARS Polyclonal Antibody (A4182) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..