Contents

Part 1 DNA TUMOR

Symposium Participants V Foreword xvii

Viral Oncogenes H. M. Temin

Synopses

Transforming of DNA Tumor Viruses L. V. Crawford 9 Transforming Genes of P.H. Duesberg 13

DNA Tumor Viruses: Early and Early Proteins

Transcription of the SV40 Genome in -transformed Cells and Early Lytic Infection P. K. Ghosh, M. Piatak, V. B. Reddy, J. Swinscoe, P. Lebowitz, and S. M. Weissman 31 Regulation of SV40 Expression through RNA Splicing G. Khoury, J. C. Alwine, R. Dhar, P. Gruss, C-J. Lai, S. Segal, and I. Seif 41 A Third Splice Site in SV40 Early mRNA D.F. Mark and P. Berg 55 Comparison of Polyoma Virus Transcription in Productively Infected Mouse Cells and Transformed Rodent Cell Lines R. Kamen, J. Favalom, J. Parker, R. Treisman, L. Lania, M. Fried, and A. Mellor 63 Transcription of SV40 DNA in Transformed Rat Cells M. Grigoryan, P. Chumakov, and E. Lukanidin 77 Expression of Transforming Viral Genes in Semipermissive Cells Transformed by SV40 or Adenovirus Type 2 or Type 5 S. J. Flint and G. A. Beltz 89 Catalytic Properties of the SV40 Large T Antigen R. Tfian, A. Robbins, and R. Clark 103 Enzymatic Activities Associated with the SV40 Large T Antigen J.D. Griffin, G. Spangler, and D. M. Livingston 113 DNA Binding and Sedimentation Properties of SV40 T Antigens Synthesized In Vivo and In Vitro C. Prives, Y. Beck, D. Gidoni, M. Oren, and it. Shure 123 Organization of T Antigens in the Polyoma Virus Genome T. Hunter, M. A. Hutchinson, W. Eckhart, T. Friedmann, A. Esty, P. La Porte, and P. Deininger 131 Is Polyoma Virus Middle T Antigen a Protein Kinase? A.E. Smith, M. Fried, Y. Ito, N. Spurt, and RI Smith 141

T Antigens

Polyoma Virus T Antigens Expressed in Transformed Cells: Significance of Middle T Antigen in Transformation Y. Ito and N. Spurt 149

xix xx CONTENTS

Purification and Characterization of the SV40 F-gene Protein P. Tegtmeyer, T. Spillman, and F. R. Schuetz 159 Phosphorylation of SV40 Large T Antigen G. Walter and P. J. FIory, Jr. 165 T Antigens of SV40-transformed Cells t7. McCormick, F. Chaudry, R. Harvey, R. Smith, P. W. J. Rigby, E. Paucha, and A. E. Smith 171 Characterization of the Complex between SV40 Large T Antigen and the 53K Host Protein in Transformed Mouse Cells L. V. Crawford, D. P. Lane, D. T. Denhardt, E. E. Harlow, P. M. Nicklin, K. Osborn, and D. C. Pim 179 New Genetic Information Expressed in SV40-transformed Cells: Characterization of the 55K Proteins and Evidence for Unusual SV40 mRNAs P. May, M. Kress, M. Lange, and E. May 189 T-antigen-associated Proteins Induced by SV40 Transformation J.A. Melero, D. S. Greenspan, and R. B. Carroll 201 SV40 17K Protein Is Associated with Two Cellular Proteins K. Rundell, P. Hearing, and Y. C. Yang 211 Characterization of a Murine Cellular SV40 Antigen in SV40-transformed Cells and in Uninfected Embryonal Carcinoma Cells D. I. H. Linzer, W. Maltzman, and A. J. Levine 215 SV40 T-antigen-related Molecules on the Surfaces of HeLa Cells Infected with Adenovirus-2-SV40 Hybrids and on SV40-transformed Cells W. Deppert and R. Henning 225 SV40 Transplantation Antigen: Relationship to SV40-specific Proteins S.S. Tevethia, R. S. Greenfield, D. C. Flyer, and M. J. Tevethia 235 Host Antigens in the Centriolar Region Are Induced in SV40-infected TC7 Cells: SV40 Small-T-function Requirement H. Kasamatsu, M. Shyamala, and W. Lin 243 Transformation Induced by Epstein-Barr Virus and the Role of the Nuclear Antigen G. Klein, J. Luka, and J. Zeuthen 253

The Genetics of Transformation by DNA Tumor Viruses

The hr-t Gene of Polyoma Virus T. L. Benjamin, G. G. Carmichael, and B. S. Schaffhausen 263 Early Mutants of Polyoma Virus (dl8 and dl23) with Altered Transformation Proper- ties: Is Polyoma Virus Middle T Antigen a Transforming Gene Product? B.E. Griffin, Y. Ito, U. Novak, N. Spurt, S. Dilworth, N. Smolar, R. Pollack, K. Smith, and D. Rifkin 271 Deletion Mutants of SV40 That Affect the Structure of Viral Tumor Antigens Jr. M. Pipas, S. P. Adler, K. W. C. Peden, and D. Nathans 285 Origin-defective Mutants of SV40 Y. Gluzman, R. J. Frisque, and J. Sambrook 293 Lytic Infection of Teratocarcinoma Cells by Polyoma Virus Mutants M. Vasseur, C. Kress, N. Montreau, and D. Blangy 301 SV40 Mutant tsA1499 Is Heat-sensitive for Lytic Growth but Generates Cold-sensitive Rat-cell Transformants D. Pintel, N. Bouck, G. di Mayorca, B. Thimmappaya, B. Swerdlow, and T. Shenk 305 Roles of the T Antigens in Transformation by SV40 R.G. Martin, V. P. Setlow, A. B. Chepelinsky, R. Seif, A. M. Lewis, Jr., C. D. Scher, C. D. Stiles, and J. Avila 311 Requirement for the Large T and Small T Proteins of SV40 in the Maintenance of the Transformed State R. J. Frisque, D. B. Rifkin, and W. C. Topp 325 Host-virus Interactions Critical for Cellular Transformation by Polyoma Virus and SV40 M. Rassoulzadegan, E. Mougneau, B. Perbal, P. Gaudray, F. Birg, and F. Cuzin 333 Viral and Cellular Control of the SV40-transformed Phenotype J.B. Hiscott and V. Defendi 343 Location of Additional Early Regions within the Left Quarter of the Adenoviral Genome J. Williams, R. S. Galos, M. H. Binger, and S. J. Flint 353 Functional Analysis of Adenovirus-5 Host-range Deletion Mutants Defective for Transformation of Rat Embryo Cells T. Shenk, N. Jones, W. Colby, and D. Fowlkes 367 SV-induced Somatic Mutations: Possible Relevance to Viral Transformation M. Theile, M. Strauss, L. Luebbe, S. Scherneck, H. Krause, and E. Geissler 377 CONTENTS xxi

The Transforming Genes of Adenoviruses and Their Expression

Structure and Function of the Transforming Genes of Human Adenoviruses and SV40 A.J. van der Eb, H. van Ormondt, P. L Schrier, J. H. Lupker, H. Jochemsen, P. J. van den Elsen, R. J. DeLeys, J. Maat, C. P. van Beveren, R. Dijkema, and A. de Waard 383 RNA Transcription and Splicing at Early and Intermediate Times after Adenovirus-2 Infection L. T. Chow, J. B. Lewis, and T. R. Broker 401 Biogenesis, Structures, and Sites of Encoding of the 5' Termini of Adenovirus-2 mRNAs C. C. Baker and E. B. Ziff 415 Phenotypes of Adenovirus-5 Host-range Mutants for Early mRNA Synthesis A.J. Berk, F. Lee, T. Harrison, J. Williams, and P. A. Sharp 429 Expression of Genes from the Transforming Region of Adenovirus D.J. Spector, D. N. Halbert, L. D. Crossland, and H. J. Raskas 437 Metabolism of mRNA from the Transforming Region of Adenovirus 2 M.C. Wilson, J. R. Nevins, J.-M. Blanchard, H. S. Ginsberg, and J. E. Darnell, Jr. 447 Studies on Early Proteins and Transformation Proteins of Human Adenoviruses M. Green, W. S. M. Wold, K. Brackmann, and M. A. Cartas 457 Analysis of Cloned mRNA Sequences from the Transforming Region of Adenovirus 2 M. Perricaudet, G. Akusjiirvi, A. Virtanen, and U. Pettersson 471 Transforming Proteins of Human Adenovirus 5: Studies with Infected and Trans- formed Cells N. J. Lassam, S. T. Bayley, and F. L. Graham 477 Organization and Expression of the Left Third of the Genome of Adenovirus J.B. Lewis, H. Esche, J. E. Smart, B. W. Stillman, M. L. Hatter, and M. B. Mathews 493 Adenoviral Early Glycoprotein E3-19K and Its Association with Transplantation Antigens H. Persson, S. Kvist, L. Ostberg, P. A. Peterson, and L. Philipson 509 Nucleotide Sequences, Integration, and Transcription of the Adenovirus-12 Trans- forming Genes K. Fujinaga, Y. Sawada, Y. Uemizu, T. Yamashita, H. Shimojo, K. Shiroki, H. Sugisaki, K. Sugimoto, and M. Takanami 519 Products of the Adenovirus-12 Transforming Genes and Their Functions K. Shiroki, K. Segawa, L Saito, H. Shimojo, and K. Fujinaga 533

Transforming Genes of DNA Tumor Viruses and Their Arrangement in Transformed Cells

Viral DNA Sequences in Adenovirus-transformed Cells L. Visser, M. W. van Maar- schalkerweerd, T. H. Rozijn, A. D. C. Wassenaar, A. M. C. B. Reemst, and J. S. Sussenbach 541 Selectivity in Integration Sites of Adenoviral DNA W. Doerfler, S. Stabel, H. Ibelgaufts, D. Sutter, R. Neumann, J. Groneberg, K. H. Scheidtmann, R. Deuring, and U. Winterhoff 551 Methylation of Integrated Viral DNA Sequences in Hamster Cells Transformed by Adenovirus 12 D. Sutter and W. Doerfler 565 Analysis of the Sites of Integration of Viral DNA Sequences in Rat Cells Transformed by Adenovirus 2 or SV40 J. Sambrook, R. Greene, J. Stringer, T. Mitchison, S.-L. Hu, and M. Botchan 569 Mechanism for Circularization of MVM DNA by "Noose" Sliding in a Lassolike Structure: Implication for Integration Y. Aloni, S. Bratosin, O. Laub, and J. Tal 585 Tumorigenicity of Polyoma Virus in Hamsters M. A. Israel, K. Chowdhury, J. Ramseur, K. Chandrasekaran, D. F. Vanderryn, and M. A. Martin 591 Organization and Expression of Integrated Polyoma Virus DNA Sequences in Trans- formed Rodent Cells L. Lania, A. Hayday, G. Bjursell, D. Gandini-Attardi, and M. Fried 597 Monkey Cells Transformed by SV40 DNA Fragments: Flat Revertants Synthesize Large and Small T Antigens M. Graessmann, A. Graessmann, and C. Mueller 605 Integration and Excision of Polyoma Virus Genomes C. Basilico, D. Zouzias, G. Della-Valle, S. Gattoni, V. Colantouni, R. Fenton, and L. Dailey 611 Rapid Detection, Isolation, and Amplification of Host-substituted SV40 Variants E. Winocour, M. Singer, and E. Kuff 621 xxii CONTENTS

Morphological Transformation by DNA Fragments of Human Herpesviruses: Evi- dence for Two Distinct Transforming Regions in Herpes Simplex Virus Types 1 and 2 and Lack of Correlation with Biochemical Transfer of the Thymidine Kinase Gene G. R. Reyes, R. LaFemina, S. D. Hayward, and G. S. Hayward 629

The Transformed Cell

Sarcoma Growth Factor: Specific Binding to and Elution from Membrane Receptors for Epidermal Growth Factor J. E. DeLarco and G. J. Todaro 643 Transformation-specific Secreted Proteins D. R. Senger, D. F. Wirth, C. Bryant, and R. O. Hynes 651 A Common Transformation-related Protein in Murine Sarcomas and Leukemias G. Jay, A. B. DeLeo, E. Appella, G. C. Dubois, L. W. Law, G. Khoury, and L. J. Old 659 Regulation of Plasminogen Activator Synthesis in Chick Embryo Fibroblasts Infected with Avian Retroviruses D.B. Ri[kin 665 New Assay for the Plasminogen Activator Activity of Transformed Cells W.F. Mangel, D. C. Livingston, J. R. Brocklehurst, H.-Y. Liu, G. A. Peltz, J. F. Cannon, S. P. Leytus, J. A. Wehrly, B. L. Salter, and J. L. Mosher 669 SV40 and Cellular Gene Expression in the Maintenance of the Tumorigenic Syn- drome R. Pollack, A. Lo, B. Steinberg, K. Smith, H. Shure, G. Blanck, and M. Verderame 681 Expression of Transformation in Cell Hybrids: Analysis of a Revertant of SVT2 K. K. Jha, E. G. Gurney, L. A. FeMman, and H. L. Ozer 689 Tumor Formation by Transformed Cells in Nude Mice P. Kahn, R. S. Simon, A. S. Klein, and S. Shin 695 A Study to Determine the Reasons for Differences in the Tumorigenicity of Rat Cell Lines Transformed by Adenovirus 2 and Adenovirus 12 P.H. GaUimore and C. Paraskeva 703 Monoclonal Antibodies: Detection of Transformation-related Antigens S.S. Ved- Brat, U. Hammerling, W. D. Hardy, E. Boren[reund, and W. Prensky 715

Author Index (Part 1) li

Subject Index (Part 1) 3i

Part 2 RNA TUMOR VIRUSES

The Genomes and Proteins of Retroviruses

Organization of Murine Sarcoma Virus Genomes D.J. Donoghue, M. P. Goldfarb, P. A. Sharp, and R. A. Weinberg 721 Cellular Origin of the Transforming Gene of Moloney Murine Sarcoma Virus E. Canaani, S. R. Tronick, K. C. Robbins, P. R. Andersen, C. Y. Dunn, and S. A. Aaronson 727 Characterization of Integrated Moloney Sarcoma Proviruses and Flanking Host Sequences Cloned in Bacteriophage A G. W. Vande Woude, M. Oskarsson, W. L. McClements, L. W. Enquist, D. Blair, P. J. Fischinger, J. V. Maizel, and M. Sullivan 735 Characterization of Virus-specified Proteins Present in NRK Cells Infected with a Temperature-sensitive Transformation Mutant of Moloney Murine Sarcoma Virus T. G. Wood, J. Peltier-Horn, W. G. Robey, D. G. Blair, and R. B. Arlinghaus 747 CONTENTS xxiii

Comparative Analysis of Cellular and Viral Sequences Related to Sarcomagenic Cell Transformation L.-H. Wang, P. Snyder, T. Hana[usa, C. Moscovici, and H. Hanafusa 755 Transforming Protein Encoded by the Cellular Information of Recovered Avian Sarcoma Viruses R. E. Karess, W. S. Hayward, and H. Hana[usa 765 Correlation of Transient Accumulation of Linear Unintegrated Viral DNA and Transient Cell Killing by Avian Leukosis and Reticuloendotheliosis Viruses H. M. Temin, E. Keshet, and S. K. Weller 773 Endogenous Virogenes and Oncogenes in Rat-cell Transformation: A New Model System S. Rasheed 779 Isolation and Characterization of Proteins Carrying the Feline Oncornavirus-asso- elated Cell-membrane Antigen H. W. Snyder, Jr., K. J. Phillips, W. D. Hardy, Jr., E. E. Zuckerman, M. Essex, A. H. Sliski, and J. Rhim 787 Genetic Structure of Avian Acute Leukemia Viruses K. Bister and P. H. Duesberg 801 RNAs of Defective and Nondefective Components of Friend Anemia and Poly- cythemia Virus Strains Identified and Compared L. Evans, M. Nunn, P. Dues- berg, D. Troxler, and E. Scolnick 823 Analysis of RNA Specific for the Transforming Agent of R-strain Avian Eryth- roblastosis Virus K. Toyoshima, T. Kamahora, H. Sugiyama, M. Owada, A. Nomoto, and M. Yoshida 837 Characterization of the src Gene of a Mutant Temperature- sensitive for Cell Transformation J.-L. Darlix, P. A. Bromley, and P.-E Spahr 843 Structure and Expression of the Abelson Genome and Its Relationship to a Normal Cell Gene D. Baltimore, A. Shields, G. Otto, S. Goff, P. Besmer, O. Witte, and N. Rosenberg 849 A Trans-acting Protein Kinase Identified in Cells Transformed by Abelson Murine Leukemia Virus O. N. Witte, L. Sun, N. Rosenberg, and D. Baltimore 855 Characterization of Abelson Murine Leukemia Virus Isolates Differing in P120 Expression N. Rosenberg, O. N. Witte, and D. Baltimore 859 Mammalian RNA Type-C Transforming Viruses: Characterization of Virus-coded Polyproteins Containing Phosphorylated Components with Possible Transforming Functions J. R. Stephenson, W. J. M. Van de Ven, A. S. Khan, and F. H. Reynolds, Jr. 865 RNA Sequences and Proteins Specific to Friend Strain of Spleen Focus-forming Virus Y. lkawa, Y. Kobayashi, M. Obinata, F. Harada, S. Hino, and H. Yoshikura 875 Progressive Shutdown of Viral Gene Expression in Friend-virus-induced Eryth- roleukemia Cells A. S. Berkower, F. Plata, R. Anand, F. Lilly, R. Soeiro, and R. A. Steeves 887 Host Control of Sequences Specific to Friend Erythroleukemia Virus in Normal and Leukemic Mice T. W. Mak, C. L. Gamble, M. E. MacDonald, and A. Bernstein 893 Moloney Murine Sarcoma Virions Contain Subgenomic-length mRNA-like Molecules That Direct the Synthesis of 'Sarcoma-specific' Polypeptides In Vitro D. Dina and B. Nadal-Ginard 901 src

Protein Phosphorylation Mediated by Partially Purified Avian Sarcoma Virus Transforming-gene Product R. L. Erikson, M. S. Collett, E. Erikson, A. F. Purchio, and J. S. Brugge 907 Origin and Function of Avian Transforming Genes J.M. Bishop, S. A. Courtneidge, A. D. Levinson, H. Oppermann, N. Quintrell, D. K. Sheiness, S. R. Weiss, and H. E. Varmus 919 Studies on the Structure and Function of the Avian Sarcoma Virus Transforming-gene Product T. Hunter, B. M. Se[ton, and K. Beemon 931 In Vitro Synthesis and Processing of Rous Sarcoma Virus src-gene Products J. Kamine, J. G. Burr, and J. M. Buchanan 943 Activities of the src-gene Product of Avian Sarcoma Virus P.F. Maness, H. Engeser, M. E. Greenberg, M. O'Farreli, W. E. Gall, and G. M. Edelman 949 xxiv CONTENTS

Comparison of Protein Kinase Activities in Normal Cells and Cells Transformed by a Temperature-sensitive Mutant of Avian Sarcoma Virus to Those of Cell-free Viral Translational Products M. Owada, P. Donner, K. E. J. Dittmar, and K. Moelling 959 F-actin Inhibits Protein Kinase Activity Associated with the src-gene Product S.D. Tsen, R. Lee, B. Damiani, P. Hsieh, and L. B. Chen 967 Transformation by Rous Sarcoma Virus: Effects of src-gene Expression on the Synthesis and Phosphorylation of Cellular Polypeptides K. Radke and G. S. Martin 975 Expression of Viral Oncogenes in Differentiating Chick Embryo Neuroretinal Cells Infected with Avian Tumor Viruses G. Calothy, F. Poirier, G. Dambrine, P. Mignatti, P. Combes, and B. Pessac 983 Localization and Characterization of the src-gene Product of Rous Sarcoma Virus A. R. Goldberg, J. G. Krueger, and E. Wang 991 Transformation-defective, Temperature-sensitive Mutants of Rous Sarcoma Virus Have a Reversibly Defective src-gene Product R. R. Friis, B. M. Jockusch, C. B. Boschek, A. Ziemiecki, H. Riibsamen, and H. Bauer 1007 Localization of pp60~'c within Normal Rat Kidney Cells Infected with Temperature- sensitive Mutants if-class) of Rous Sarcoma Virus L.R. Rohrschneider 1013 Nature and Intracellular Location of the Product of the src Gene of Avian Sarcoma Virus I. Pastan, G. Jay, N. Richert, P. J. A. Davies, and M. Willingham 1023 Genetic and Biochemical Approaches to Analyzing Transformation by Rous Sarcoma Virus D. D. Anderson, D. W. Salter, L. R. Rohrschneider, and M. J. Weber 1031 Isolation of a Glucose Transport System from Normal Chicken Fibroblasts and from Chicken Fibroblasts Transformed by Rous Sarcoma Virus F. Lipmann and S. G. Lee 1043 Growth-Related Alterations Induced in Chick Embryo Fibroblasts by src-gene Deletion Mutants of the Schmidt-Ruppin Strain of Rous Sarcoma Virus M.L. Breitman, R. Vigne, and P. K. Vogt 1047 Expression of the src-gene Product, pp6tY"~, in Transformed and Reverted Mammalian Cells Infected with Avian Sarcoma Virus A. F. Lau, R. A. Krzyzek, J. S. Brugge, M. S. Collett, R. L. Erikson, and A. J. Faras 1057 Factors Affecting Phenotypic Reversion of Rat Cells Transformed by Avian Sarcoma Virus J. A. Wyke, J. A. Beamand, and H. E. Varmus 1065

Proviruses and Their Expression

Organization of Endogenous and Exogenous Viral and Linked Nonviral Sequences S. H. Hughes, P. K. Vogt, E. Stubblefield, H. Robinson, J. M. Bishop, and H. E. Varmus 1077 Synthesis and Integration of Avian Sarcoma Virus DNA J.M. Taylor, T. W. Hsu, C. Yeater, and W. S. Mason 1091 Avian Endogenous Proviral DNA: Analysis of Integrated ev 1 and a Related gs-chf- Provirus Purified by Molecular Cloning A. Skalka, P. DeBona, F. Hishinuma, and W. McClements 1097 Ten Genetic Loci in the Chicken That Contain Structural Genes for Endogenous Avian Leukosis Viruses S. M. Astrin, H. L. Robinson, L. B. Crittenden, E. G. Buss, J. Wyban, and W. S. Hayward 1105 Transcriptional Products and DNA Structure of Endogenous Avian Proviruses W.S. Hayward, S. B. Braverman, and S. M. Astrin 1111 Role of the C Region in Relative Growth Rates of Endogenous and Exogenous Avian P. N. Tsichlis and J. M. Coffin 1123 Subgroup-E Avian-leukosis-virus-associated Disease in Chickens H.L. Robinson, M. N. Pearson, D. W. DeSimone, P. N. Tsichlis, and J. M. Coffin 1133 Structure and Infectivity of Integrated Murine Leukemia Virus DNA D.R. Lowy, E. Rands, S. K. Chattopadhyay, and G. L. Hager 1143 Construction and Characterization of Molecular Clones Containing Integrated Mouse Mammary Tumor Virus Sequences L. A. Donehower, J. Andre, D. S. Berard, R. G. Wolford, and G. L. Hager 1153 Genomic Location of Mouse Mammary Tumor Virus Proviral DNA in Normal Mouse Tissue and in Mammary Tumors N. E. Hynes, B. Groner, H. Diggelmann, R. Van Nie, and R. Michalides 1161 CONTENTS XXV

Transformation of NIH-3T3 Mouse Cells by Avian Retroviral DNAs G. M. Cooper, N. G. Copeland, A. D. Zelenetz, and T. Kronfiris 1169

The Transformed Phenotype and Differentiation

Control of Proliferation and Differentiation in Cells Transformed by Friend Virus D. Housman, R. Levenson, V. Volloch, A. Tsiftsoglou, J. Gusella, D. Parker, J. Kernen, A. Mitrani, V. Weeks, O. Witte, and P. Besmer 1177 Transformation by Abelson Murine Leukemia Virus: Properties of the Transformed Cells W. C. Raschke 1187 Cell-surface Antigens of Abelson and Friend Murine Leukemia Viruses and of Hematopoietic Differentiation R. Risser, D. Grunwald, C. Sinaiko, and P. Jelen 1195 Normal B-cell Activation Involves Endogenous Retroviral Antigen Expression: Im- plications for Leukemogenesis C. Moroni, J. P. Stoye, J. F. DeLamarter, P. Erb, F. A. Jay, J. Jongstra, D. Martin, and G. Schumann 1205 In Vitro Transformation of Mouse Hematopoietic Cells by the Spleen Focus-forming Virus W. D. Hankins, S. B. Krantz, T. A. Kost, and M. J. Koury 1211 Three New Types of Viral Oncogenes in Defective Avian Leukemia Viruses. I. Specific Nucleotide Sequences of Cellular Origin Correlate with Specific Trans- formation D. St~helin, S. Saule, M. Roussel, A. Sergeant, C. Lagrou, C. Rom- mens, and M. B. Raes 1215 Three New Types of Viral Oncogenes in Defective Avian Leukemia Viruses. II. Biological, Genetic, and Immunochemical Evidence T. Graf, H. Beug, A. yon Kirchbach, and M. J. Hayman 1225 Recombination in the Avian Oncoviruses as a Model for the Generation of Defective Transforming Viruses R. N. Eisenman, M. Linial, M. Groudine, R. Shaikh, S. Brown, and P. E. Neiman 1235 Progenitor-cell Populations Can Be Infected by RNA Tumor Viruses, but Trans- formation is Dependent on the Expression of Specific Differentiated Func- tions D. Boettiger and E. M. Durban 1249

Viruses of Thymic Leukemia

Murine-leukemia-virus-related CeU-surface Antigens as Serological Markers of AKR Eeotropic, Xenotropic, and Dualtropic Viruses P. V. O'Donnell, E. Stockert, Y. Obata, A. B. DeLeo, and L. J. Old 1255 Status of the Association of Mink Cell Focus-forming Viruses with Leukemogenesis W. P. Rowe, M. W. Cloyd, and J. W. Hartley 1265 Analysis of the Genomes of Mink Cell Focus-forming Murine Type-C Viruses: A Progress Report M.L. Lung, C. Hering, J. W. Hartley, W. P. Rowe, and N. Hopkins 1269 Nucleotide Sequence of a Cloned Murine Leukemia Virus DNA Fragment J.G. Sutcliffe, T. M. Shinnick, R. A. Lerner, P. Johnson, and L M. Verma 1275 Murine Leukemia Virus env-gene Expression in Preleukemic Thymocytes and Leukemia Cells of AKR Strain Mice N. G. Famulari, J.-S. Tung, P. V. O'Don- nell, and E. Fleissner 1281 Evolution of Oncogenic Mouse Type-C Viruses in Cell Culture U.R. Rapp and G. J. Todaro 1289 T-lymphoma Retroviral Receptors and Control of T-lymphoma Cell Proliferation M. S. McGrath, E. Pillemer, D. A. Kooistra, S. Jacobs, L. Jerabek, and L L. Weissman 1297

Summary

Molecular Biology of Viral Oncogenes P. A. Sharp I305

Author Index (Part 2) li

Subject Index (Part 2) 3i