Contents Part 1 DNA TUMOR VIRUSES Symposium Participants V Foreword xvii Viral Oncogenes H. M. Temin Synopses Transforming Genes of DNA Tumor Viruses L. V. Crawford 9 Transforming Genes of Retroviruses P.H. Duesberg 13 DNA Tumor Viruses: Early RNAs and Early Proteins Transcription of the SV40 Genome in Virus-transformed Cells and Early Lytic Infection P. K. Ghosh, M. Piatak, V. B. Reddy, J. Swinscoe, P. Lebowitz, and S. M. Weissman 31 Regulation of SV40 Gene Expression through RNA Splicing G. Khoury, J. C. Alwine, R. Dhar, P. Gruss, C-J. Lai, S. Segal, and I. Seif 41 A Third Splice Site in SV40 Early mRNA D.F. Mark and P. Berg 55 Comparison of Polyoma Virus Transcription in Productively Infected Mouse Cells and Transformed Rodent Cell Lines R. Kamen, J. Favalom, J. Parker, R. Treisman, L. Lania, M. Fried, and A. Mellor 63 Transcription of SV40 DNA in Transformed Rat Cells M. Grigoryan, P. Chumakov, and E. Lukanidin 77 Expression of Transforming Viral Genes in Semipermissive Cells Transformed by SV40 or Adenovirus Type 2 or Type 5 S. J. Flint and G. A. Beltz 89 Catalytic Properties of the SV40 Large T Antigen R. Tfian, A. Robbins, and R. Clark 103 Enzymatic Activities Associated with the SV40 Large T Antigen J.D. Griffin, G. Spangler, and D. M. Livingston 113 DNA Binding and Sedimentation Properties of SV40 T Antigens Synthesized In Vivo and In Vitro C. Prives, Y. Beck, D. Gidoni, M. Oren, and it. Shure 123 Organization of T Antigens in the Polyoma Virus Genome T. Hunter, M. A. Hutchinson, W. Eckhart, T. Friedmann, A. Esty, P. La Porte, and P. Deininger 131 Is Polyoma Virus Middle T Antigen a Protein Kinase? A.E. Smith, M. Fried, Y. Ito, N. Spurt, and RI Smith 141 T Antigens Polyoma Virus T Antigens Expressed in Transformed Cells: Significance of Middle T Antigen in Transformation Y. Ito and N. Spurt 149 xix xx CONTENTS Purification and Characterization of the SV40 F-gene Protein P. Tegtmeyer, T. Spillman, and F. R. Schuetz 159 Phosphorylation of SV40 Large T Antigen G. Walter and P. J. FIory, Jr. 165 T Antigens of SV40-transformed Cells t7. McCormick, F. Chaudry, R. Harvey, R. Smith, P. W. J. Rigby, E. Paucha, and A. E. Smith 171 Characterization of the Complex between SV40 Large T Antigen and the 53K Host Protein in Transformed Mouse Cells L. V. Crawford, D. P. Lane, D. T. Denhardt, E. E. Harlow, P. M. Nicklin, K. Osborn, and D. C. Pim 179 New Genetic Information Expressed in SV40-transformed Cells: Characterization of the 55K Proteins and Evidence for Unusual SV40 mRNAs P. May, M. Kress, M. Lange, and E. May 189 T-antigen-associated Proteins Induced by SV40 Transformation J.A. Melero, D. S. Greenspan, and R. B. Carroll 201 SV40 17K Protein Is Associated with Two Cellular Proteins K. Rundell, P. Hearing, and Y. C. Yang 211 Characterization of a Murine Cellular SV40 Antigen in SV40-transformed Cells and in Uninfected Embryonal Carcinoma Cells D. I. H. Linzer, W. Maltzman, and A. J. Levine 215 SV40 T-antigen-related Molecules on the Surfaces of HeLa Cells Infected with Adenovirus-2-SV40 Hybrids and on SV40-transformed Cells W. Deppert and R. Henning 225 SV40 Transplantation Antigen: Relationship to SV40-specific Proteins S.S. Tevethia, R. S. Greenfield, D. C. Flyer, and M. J. Tevethia 235 Host Antigens in the Centriolar Region Are Induced in SV40-infected TC7 Cells: SV40 Small-T-function Requirement H. Kasamatsu, M. Shyamala, and W. Lin 243 Transformation Induced by Epstein-Barr Virus and the Role of the Nuclear Antigen G. Klein, J. Luka, and J. Zeuthen 253 The Genetics of Transformation by DNA Tumor Viruses The hr-t Gene of Polyoma Virus T. L. Benjamin, G. G. Carmichael, and B. S. Schaffhausen 263 Early Mutants of Polyoma Virus (dl8 and dl23) with Altered Transformation Proper- ties: Is Polyoma Virus Middle T Antigen a Transforming Gene Product? B.E. Griffin, Y. Ito, U. Novak, N. Spurt, S. Dilworth, N. Smolar, R. Pollack, K. Smith, and D. Rifkin 271 Deletion Mutants of SV40 That Affect the Structure of Viral Tumor Antigens Jr. M. Pipas, S. P. Adler, K. W. C. Peden, and D. Nathans 285 Origin-defective Mutants of SV40 Y. Gluzman, R. J. Frisque, and J. Sambrook 293 Lytic Infection of Teratocarcinoma Cells by Polyoma Virus Mutants M. Vasseur, C. Kress, N. Montreau, and D. Blangy 301 SV40 Mutant tsA1499 Is Heat-sensitive for Lytic Growth but Generates Cold-sensitive Rat-cell Transformants D. Pintel, N. Bouck, G. di Mayorca, B. Thimmappaya, B. Swerdlow, and T. Shenk 305 Roles of the T Antigens in Transformation by SV40 R.G. Martin, V. P. Setlow, A. B. Chepelinsky, R. Seif, A. M. Lewis, Jr., C. D. Scher, C. D. Stiles, and J. Avila 311 Requirement for the Large T and Small T Proteins of SV40 in the Maintenance of the Transformed State R. J. Frisque, D. B. Rifkin, and W. C. Topp 325 Host-virus Interactions Critical for Cellular Transformation by Polyoma Virus and SV40 M. Rassoulzadegan, E. Mougneau, B. Perbal, P. Gaudray, F. Birg, and F. Cuzin 333 Viral and Cellular Control of the SV40-transformed Phenotype J.B. Hiscott and V. Defendi 343 Location of Additional Early Regions within the Left Quarter of the Adenoviral Genome J. Williams, R. S. Galos, M. H. Binger, and S. J. Flint 353 Functional Analysis of Adenovirus-5 Host-range Deletion Mutants Defective for Transformation of Rat Embryo Cells T. Shenk, N. Jones, W. Colby, and D. Fowlkes 367 SV-induced Somatic Mutations: Possible Relevance to Viral Transformation M. Theile, M. Strauss, L. Luebbe, S. Scherneck, H. Krause, and E. Geissler 377 CONTENTS xxi The Transforming Genes of Adenoviruses and Their Expression Structure and Function of the Transforming Genes of Human Adenoviruses and SV40 A.J. van der Eb, H. van Ormondt, P. L Schrier, J. H. Lupker, H. Jochemsen, P. J. van den Elsen, R. J. DeLeys, J. Maat, C. P. van Beveren, R. Dijkema, and A. de Waard 383 RNA Transcription and Splicing at Early and Intermediate Times after Adenovirus-2 Infection L. T. Chow, J. B. Lewis, and T. R. Broker 401 Biogenesis, Structures, and Sites of Encoding of the 5' Termini of Adenovirus-2 mRNAs C. C. Baker and E. B. Ziff 415 Phenotypes of Adenovirus-5 Host-range Mutants for Early mRNA Synthesis A.J. Berk, F. Lee, T. Harrison, J. Williams, and P. A. Sharp 429 Expression of Genes from the Transforming Region of Adenovirus D.J. Spector, D. N. Halbert, L. D. Crossland, and H. J. Raskas 437 Metabolism of mRNA from the Transforming Region of Adenovirus 2 M.C. Wilson, J. R. Nevins, J.-M. Blanchard, H. S. Ginsberg, and J. E. Darnell, Jr. 447 Studies on Early Proteins and Transformation Proteins of Human Adenoviruses M. Green, W. S. M. Wold, K. Brackmann, and M. A. Cartas 457 Analysis of Cloned mRNA Sequences from the Transforming Region of Adenovirus 2 M. Perricaudet, G. Akusjiirvi, A. Virtanen, and U. Pettersson 471 Transforming Proteins of Human Adenovirus 5: Studies with Infected and Trans- formed Cells N. J. Lassam, S. T. Bayley, and F. L. Graham 477 Organization and Expression of the Left Third of the Genome of Adenovirus J.B. Lewis, H. Esche, J. E. Smart, B. W. Stillman, M. L. Hatter, and M. B. Mathews 493 Adenoviral Early Glycoprotein E3-19K and Its Association with Transplantation Antigens H. Persson, S. Kvist, L. Ostberg, P. A. Peterson, and L. Philipson 509 Nucleotide Sequences, Integration, and Transcription of the Adenovirus-12 Trans- forming Genes K. Fujinaga, Y. Sawada, Y. Uemizu, T. Yamashita, H. Shimojo, K. Shiroki, H. Sugisaki, K. Sugimoto, and M. Takanami 519 Products of the Adenovirus-12 Transforming Genes and Their Functions K. Shiroki, K. Segawa, L Saito, H. Shimojo, and K. Fujinaga 533 Transforming Genes of DNA Tumor Viruses and Their Arrangement in Transformed Cells Viral DNA Sequences in Adenovirus-transformed Cells L. Visser, M. W. van Maar- schalkerweerd, T. H. Rozijn, A. D. C. Wassenaar, A. M. C. B. Reemst, and J. S. Sussenbach 541 Selectivity in Integration Sites of Adenoviral DNA W. Doerfler, S. Stabel, H. Ibelgaufts, D. Sutter, R. Neumann, J. Groneberg, K. H. Scheidtmann, R. Deuring, and U. Winterhoff 551 Methylation of Integrated Viral DNA Sequences in Hamster Cells Transformed by Adenovirus 12 D. Sutter and W. Doerfler 565 Analysis of the Sites of Integration of Viral DNA Sequences in Rat Cells Transformed by Adenovirus 2 or SV40 J. Sambrook, R. Greene, J. Stringer, T. Mitchison, S.-L. Hu, and M. Botchan 569 Mechanism for Circularization of MVM DNA by "Noose" Sliding in a Lassolike Structure: Implication for Integration Y. Aloni, S. Bratosin, O. Laub, and J. Tal 585 Tumorigenicity of Polyoma Virus in Hamsters M. A. Israel, K. Chowdhury, J. Ramseur, K. Chandrasekaran, D. F. Vanderryn, and M. A. Martin 591 Organization and Expression of Integrated Polyoma Virus DNA Sequences in Trans- formed Rodent Cells L. Lania, A. Hayday, G. Bjursell, D. Gandini-Attardi, and M. Fried 597 Monkey Cells Transformed by SV40 DNA Fragments: Flat Revertants Synthesize Large and Small T Antigens M. Graessmann, A. Graessmann, and C. Mueller 605 Integration and Excision of Polyoma Virus Genomes C. Basilico, D. Zouzias, G. Della-Valle, S. Gattoni, V. Colantouni, R. Fenton, and L. Dailey 611 Rapid Detection, Isolation, and Amplification of Host-substituted SV40 Variants E. Winocour, M. Singer, and E. Kuff 621 xxii CONTENTS Morphological Transformation by DNA Fragments of Human Herpesviruses: Evi- dence for Two Distinct Transforming Regions in Herpes Simplex Virus Types 1 and 2 and Lack of Correlation with Biochemical Transfer of the Thymidine Kinase Gene G.