Proposal to Create a Center for Molecular Plant Sciences

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PROPOSAL TO CREATE A CENTER FOR MOLECULAR PLANT SCIENCES

I. Long-term Objective. The success of the interdisciplinary Plant Molecular Biology/Biotechnology (PMBB) Program has evolved to the point that a College-wide (CBMPS and CFAES) Center for Molecular Plant Sciences is needed to better focus interdisciplinary efforts in the molecular plant sciences at OSU. A formalized Center would be a natural and desired progression from the successful interdisciplinary dual- college PMBB Program effort initiated nearly 15 years ago.

II. Introduction. Basic plant sciences at The Ohio State University, particularly molecular-based studies, are very strong and are highly ranked amongst land-grant universities. The majority of faculty conducting plant-related research reside in various departments of the new College of Biological, Mathematical and Physical Sciences (CBMPS) and College of Food, Agricultural, and Environmental Sciences (CFAES). Other colleges, i.e., Pharmacy, Medicine, and Engineering, also contribute to the overall excellence in plant-related research on campus. Several years ago (mid 1990’s), the interdisciplinary Plant Molecular Biology/Biotechnology (PMBB) Program was created to provide leadership in accelerating the trend towards interdisciplinary plant molecular biology research. To date, PMBB is a voluntary association created by a grass-roots effort of faculty from both Colleges. The Deans and Central Administration provided strategic support for the PMBB Program at its inception. PMBB, along with the Plant Biotechnology Center, and the Plant-Microbe Genomics Facility, now consists of a vibrant, productive, and collegial community of 29 plant-related researchers. Early on, PMBB successfully competed for positions supported by the Molecular Life Sciences initiative. The resulting 6 strong hires in turn stimulated the participating departments to contribute additional positions. This resulted in the two Colleges adding several additional stellar scientists in the field of molecular plant science. More recently, PMBB was awarded a Targeted Initiative in Excellence in Translational Plant Sciences (TIE- TPS) by OSU. This ongoing initiative has already led to the hiring of two new faculty, with three additional faculty searches that are currently underway. Moreover, PMBB members have garnered extremely competitive high visibility grants (from the Gates Foundation, NSF’s 2010 Project and Plant Genome Program, USDA-NRI, DOE Genomes to Life, and Ohio’s Third Frontier Program). The total expenditures of PMBB member grants exceeded $9 million in FY07. Clearly, PMBB faculty members lead active and productive research programs, also exemplified by published work in prestigious general journals such as Cell, Nature, Science, Proceedings of the National Academy of Sciences, Current Biology and EMBO Journal as well as top journals in their respective fields including Plant Cell, Plant Physiology, Applied and Environmental Microbiology, and the Journal of Biological Chemistry. PMBB members train large numbers of graduate students and many of these individuals have played significant roles in contributing to the overall productivity of PMBB member laboratories. The PMBB community has also been instrumental in driving the formation of several interdepartmental and inter-campus instrumentation centers to optimize the cost effective productivity of the plant science community. These include the Molecular and Cellular Imaging Center (MCIC) at the Wooster Campus, the Plant Microbe Genomics Facility (PMGF) at the Columbus Campus, and the beginning of a Metabolomics Laboratory branch of PMGF in Rightmire Hall. Recently another resource for shared instrumentation was developed in Kottman Hall on Columbus Campus that includes an additional node for genomics and metabolomics work. Likewise, PMBB has endorsed and led several efforts over the past few years to increase the video-linking capacity of member departments and the linking of seminars

1 and teaching of classes between Wooster and Columbus campuses. This latter effort has greatly enhanced enrollment in our classes and the productivity of our graduate students in that they no longer are burdened by extensive travel which is disruptive to their thesis research. Video-linking courses taught by PMBB faulty has also tremendously enhanced the educational breath of the Wooster students, helping insure their competitiveness in future job prospects. This video-linking capability will also play an important role in the development of our novel international tripartite graduate program with the University of Sao Paulo and Rutgers University (fully described in TIE- related documents).

III. Rationale. PMBB does not receive an annual budget and the one-time funding associated with the establishment of PMBB will soon run out. As noted above, PMBB has been a very productive venture, bringing in many new top-notch faculty with high profile research programs and extramural funding. In addition, PMBB has served as a focal point for the development of several interdisciplinary and cooperative research and graduate training efforts. In light of the current university-wide strategic planning process and the planned merger of the departments of Plant Cellular and Molecular Biology and Molecular Genetics, we thus feel it is an appropriate time to reaffirm college commitments to PMBB through the establishment of a more formal college-wide Center for Molecular Plant Sciences (CMPS). The Center will maintain and strengthen the momentum achieved by the highly productive PMBB Program by providing a permanently funded and stable umbrella organization. The organizational structure of a Center is critical in maintaining a strong plant molecular biology community focused on cutting-edge basic and applied plant research and the training of graduate students. The Center will also be an important recruitment tool when hiring future faculty and also play an important role in retaining our current faculty. In addition, a formalized Center will be a much more effective vehicle to mobilize other researches on campus that work with plant systems, and whose research capabilities might be effectively merged with the efforts of current PMBB members. For example, there is a strong natural products group in the College of Pharmacy that works closely with current PMBB members and several members of the PMBB group have initiated joint efforts with colleagues in Engineering. Certainly the new divisional structure in CBMPS has already facilitated closer collaborations between the Department of Chemistry and the current Translational Plant Sciences TIE. This collaboration has already paid dividends in that CBMPS, CFAES and Engineering successfully collaborated on an Ohio Research Scholars Proposal. The ORSP submission proposed hiring a new faculty member in FAES to engineer new plants which are rich in organic chemicals, a faculty member in synthetic Organic Chemistry who will transform plant-derived chemicals into versatile new building blocks and a faculty member in Engineering who can develop new methods of polymerization of the renewable and synthetically transformed feedstock chemicals. ORSP funded two endowed faculty positions and the three colleges will collaborate on self-funding the third. These types of collaborations will be much more prevalent and maximized with the establishment of a formalized Center that could focus on such joint efforts. Finally, we also envision that a strong molecular plant science Center at OSU will provide the needed leadership in state-wide efforts in emerging fields such as plant biotechnology, phytopharmaceuticals, biofuels, and alternative energy-related projects.

IV. Administration of the Center/Governance and Oversight Structure. Currently, the PMBB program is headed by a Director, who serves for a three year term, responsible to the CBMPS and CFAES Deans. Dean Matt Platz (CBS) is Lead Dean for

2 the current Translational Plant Sciences TIE, and thus is ultimately responsible for fiscal oversight; we envision that some form of similar agreement may be established with the two Colleges who would administer the proposed new Center. In addition, in the current PMBB Program, a PMBB Council consisting of four PMBB faculty is elected by the PMBB Program members. Each member of the Council serves two years before rotating off. Membership is staggered so that two new Council members are elected to replace two out-going members each year. This assures continuity of leadership on Council, with the Director as an ex-officio member. The Council was established to assist and form a communication bridge between the Director and the PMBB members. In particular, the Council helps to organize and monitor the activities of the various committees that are responsible for PMBB-related functions. We propose that a similar arrangement be established to govern the new Molecular Plant Sciences Center. All proposed actions that are to be made relative to the Center will first be vetted by the Director and Council and then brought to the whole Center membership for discussion and ratification. Good examples that well illustrate this approach are the new Graduate Fellowship and SURE undergraduate research programs established by PMBB, along with recent and current planning for the upcoming technical personnel hires and equipment purchases through the TIE. As in PMBB, all faculty hires supported by the Center will be accomplished using Search Committees comprised of Center members along with other individuals from the different TIU departments. The entire Center membership will then vote on prospective candidates. This modus operandi, in addition to college specific policies on faculty hiring, has worked well over the years for all PMBB activities and hires and will be continued in the future upon the establishment of the Center. Our governance procedures are also flexible enough to easily incorporate any changes as needed.

V. Strategic Plan and Budgetary Considerations. To implement the concept of a Center for Molecular Plant Sciences, it will be necessary to attract a Director and obtain stable funding for staff positions and operating expenses. A. CMPS Director. We request funds to recruit an outside Director for the proposed new Center (CMPS). This recruitment should be initiated as soon as possible. In addition, the hiring of such an individual would also help to assuage the loss of other recent senior molecular plant scientists. Indeed, the former PCMB Department recently attempted to hire a senior-level molecular plant scientist in 2006-2007; thus the recruitment of the Director could be looked upon as an extension of this past recruitment effort by the College. The term of current PMBB Director Robert Tabita expires in September of 2010. Thus, this is an excellent time to initiate the recruitment of a new Director. We will look for an outstanding scientist with an active research program, world-class reputation, preferably someone with administrative experience, and a person with genuine passion to lead and help build the highest caliber interdisciplinary plant science community. It would be desirable that this person’s research interests are in a field related to the recent TIE. The Director will effectively develop and implement activities to enhance interactions among the Center labs and provide visional leadership to recruit faculty and compete for large-scale funding that will place OSU at the leading edge of plant research. The Director will also lead collaborative efforts among the plant community and other scientific communities on campus, such as pharmacy, chemistry, food science, engineering, etc, to develop innovative programs addressing fundamental medical, nutrition, and energy issues. The Director will also assist and enhance on-going innovative programs and international collaborations between OSU and other institutions in plant research. These collaborations are a major part of the Translational Plant Sciences TIE and current Regents Innovation funds. it is expected that the new Director

3 will take the lead in enhancing these programs and securing renewal funding when the current TIE expires. To attract such a person, a major investment in start-up funding, research space, and other incentives will be required. While expensive, this new Director hire could be viewed as offsetting the recent loss of senior molecular plant scientists. In addition, other inducements should be considered to attract such an individual. Certainly, realized and contemplated junior hires initiated with the current TIE should be attractive for a new Director. Moreover, the establishment of this Center should stimulate agreements for other future joint faculty positions that might be considered in conjunction with other departments such as Chemistry, Engineering, Pharmacy, Nutrition, and Medicine. The advent of such innovative position hires would serve as a strong inducement to attract an outside Director and could be established with current resources for the hiring of future new faculty. To provide the Director with adequate support for his/her research program while the Director focuses on Center activities, it is recommended that the equivalent of a senior postdoctoral research scientist position be provided to help run the Director’s research program. In addition, it is recommended that 2 months summer salary be provided to the Director. B. Administrative Staff ($125,000 salary plus benefits/year). 1. Administrative Associate. We request continuing support for a full-time Administrative Associate staff member ($85,000 salary plus benefits per year) who will have wide ranging responsibilities including assisting the Director, serving as a grants coordinator for Center faculty, and acting as a liaison between Center faculty and the Office of Technology, Licensing and Contracts. Up to this point, PMBB has been operating exclusively through the associated faculty, who have taken management responsibilities for the program and a part-time Associate who has HR responsibilities for other units such as PMGF and Plant Biotechnology. With the large number of faculty associated with an expanded Center for Molecular Plant Sciences, a full-time Administrative Associate is critical for maintaining and enhancing the productivity of the faculty. The main responsibility for this staff member will be supporting the efforts of the Director to coordinate Center faculty efforts and other housekeeping tasks of the Center, such as coordinating the annual Symposium, Distinguished Seminar Program, retreat, etc. The Administrative Associate will also serve to assist the Director and Center faculty in assembling grant proposals which are submitted through Center team efforts. Finally, the Administrative Associate will serve as a liaison with the Office of Technology, Licensing and Contracts to assist Center faculty with contracts, patent applications and licensing of IP. The Molecular Plant Sciences Center is currently poised to impact both basic and applied plant sciences. Basic plant science is enhanced through basic molecular plant science research, followed by information exchange within the scientific community. Applied plant science continues to shift from the University to industry, which has greater resources and the infrastructure to commercialize products. The role of the university in the molecular plant sciences has changed but the creative science that exists at the core of the Center has tremendous potential for commercialization, once IP is secured. Center faculty have not only generated high impact publications but also hold numerous patents, many of which have been licensed for eventual commercialization of an industrial product. Unfortunately, some opportunities for IP have not been realized. The faculty and the University would benefit from additional assistance in this area, through assignment of support staff. The Administrative Associate would not be an inventor, but would facilitate assembly of patent disclosures, assist in the submission of data/documentation to the office of TLC, and help to identify or contact potential licensees.

4 2. Outreach and engagement specialist/graduate and undergraduate coordinator (half-time position at $40,000 salary plus benefits/year). In a study performed by NASA in 2000, it was shown that most children lose interest in science and math by the third grade. The effect of this is currently being seen as the level of scientific literacy in the general population has eroded and the number of college students entering the STEM disciplines has declined. To address this problem, in addition to providing leadership to Ohio’s plant molecular biology research community, the CMPS Center will have as one of its core missions to engage K-12 students and teachers and institutions serving primarily minority students, with the goals increasing the overall scientific literacy in the state of Ohio, increasing the number of students at all levels entering college in a STEM discipline, and driving up the number of women and minorities going into STEM majors at the undergraduate and graduate level. To facilitate continual and meaningful interactions with these students, we request continuing support for a half-time staff position ($40,000 salary/year plus benefits) for an Outreach and Engagement Coordinator. Duties for this position will be the following: 1. Identify opportunities within OSU and throughout the state for center faculty to interact with K-12 students and institutions serving primarily minority or tribal communities. 2. Work with faculty as they develop research grants to incorporate appropriate outreach and engagement objectives that will enhance those of the center. 3. Identify key areas where center faculty can have an impacting effect on the level of science literacy and interest in the STEM disciplines. 4. Connect Center faculty with educators throughout Ohio to foster partnerships in STEM education in the state. Initiatives such as this to promote STEM education are not new. The reason that most of these initiatives have short-lived success is that they are all too often driven by research faculty that have a limited understanding of what educators need and what students are attracted to. Many of these types of programs often run for a few short years where the continual interactions that are necessary to impart lasting change are not maintained. More times than not, faculty interested in these activities spend enormous amounts of time re-inventing the wheel and trying to identify appropriate venues to interact. The position proposed here will be an expert in the needs of Ohio’s science curriculum, they will be able to deploy center resources and faculty where they can be most effective, and they will interact with and be part of the overall efforts OSU puts towards elevating science and math in the state of Ohio. In collaboration with the Center faculty, this position can have a lasting influence on the state of science understanding and education in this state. In addition to outreach and engagement, this person will also help faculty coordinate TIE efforts in the new Translational Plant Sciences Graduate Program as well as the Summer Undergraduate Research Experience (SURE) Program described with our TIE documents. The outreach and extension specialist will also be valuable asset in providing the outreach component which is required for many of the federally-funded center grants. C. Translational Plant Science Symposium ($50,000 per year). PMBB has been holding an annual symposium for the past 10 years. The symposia were aimed at facilitating interactions among the labs in PMBB, especially among graduate students and postdoctoral fellows. The symposia have attracted other researchers from around the state of Ohio, and we routinely have used this opportunity to invite an eminent researcher as a plenary lecturer to kick off this Symposium. Because of limited funding, these activities are narrow in scope and no longer meet the exciting dynamics and

5 challenges of the molecular plant science community. Therefore, we propose a new TPS Symposium series that will combine the current PMBB symposia and further expand from a regional to international level by including participation of researchers across the country and from other parts of the world. It will feature at least 10 invited speakers of international caliber. Clearly, scientific meetings are essential for the advancement of most, if not all, research fields, in terms of sharing the latest research results, establishing collaborations, and building mutual support of each other’s work. Host institutions for meetings can gain enhanced reputation among colleagues, competitiveness in grant funding (especially large group grants), and exposure of their students/postdocs to other researchers. As an important means to boost the world prominence of OSU plant science, we request $50,000 per year as matching funds to host an international symposium on Translational Plant Science (TPS). The matching funds will help us seek additional funding from federal agencies such as NSF, USDA and DOE. The scientific theme for each year will be developed based on the recent developments in science. Examples include biofuels, biomass, disease resistance, genomics, developmental mechanisms, plant products in medicine, human nutrition, etc. Every symposium will strive to integrate basic and applied research, model and non- model plant and crop species, and invited presentations by established researchers and rising stars. Researchers from OSU and other Ohio institutions will also be invited to showcase research in the state. Such a symposium will also provide an opportunity to invite and interact with our colleagues and students associated with the tripartite graduate program with the University of Sao Paulo and Rutgers University and part of the requested budget will be used for this purpose. The CMPS will inaugurate the TPS series by sponsoring the Second International Conference on Plant Vascular Biology (PVB), which will be organized and chaired by PCMB faculty Biao Ding in 2010 on the OSU campus. At the end of the First International PVB Conference held in May 2007 in Taipei, Taiwan, OSU was selected among several competing proposals by the conference international committee to host the second conference in recognition of the international leadership and the excellence of OSU’s plant science research. Expected to be attended by approximately 300 researchers and featuring nearly 20 invited speakers from around the world, this conference will provide an exceptional opportunity to showcase plant research as OSU, elevating PMBB (or MPSC) visibility and leadership in plant research. The Samuel Roberts Noble Foundation in Oklahoma has administratively promised $10,000 to help support this conference. The Noble Foundation support and the requested OSU support will be used as critical matching funds to seek other support from federal agencies including USDA, NSF and DOE. D. Operating and Other Expenses ($50,000/year). A budget for routine office expenses, the maintenance of the PMBB Distinguished Seminar Series (which brings 3- 4 highly visible scientists to the Columbus and Wooster campuses per year), expenses relative to outreach and engagement, video-linking, web-site maintenance, faculty and student recruitment, and other miscellaneous activities is requested ($50,000/year).

6 BIOGRAPHICAL SKETCH – PIERLUIGI BONELLO

A. Appointments

2005-present Associate Professor, Department of Plant Pathology, Ohio State University, Columbus, OH 2000-2005 Assistant Professor, Department of Plant Pathology, Ohio State University, Columbus, OH

B. Professional Preparation

University of California, Davis Host-Pathogen-Insect Interactions Post-doc, 1997-2000

University of California, Berkeley Host-Pathogen-Insect Interactions; Mycorrhizal Ecology Post-doc, 1994-1996

GSF - Forschungszentrum für Umwelt und Gesundheit Air pollution and Munich, Germany Disease Resistance Post-doc, 1991-1992

University of Oxford, U.K. Forest Pathology Ph.D., 1991

University of Padova, Italy Forest Sciences "Laurea" (=M.Sc.), 1987

C. Relevant Publications – Last Four Years

Barto, E.K., S. Enright, A. Eyles, C.M. Wallis, R. Chorbadjian, R. Hansen, D.A. Herms, P. Bonello and D.F. Cipollini. 2008. Effects of soil fertility on systemic protein defense responses of austrian pine to attack by a fungal pathogen and an insect defoliator. Journal of Chemical Ecology (in press). Bonello, P., N. Luchi, P. Capretti, and M. Michelozzi. 2008. Host-mediated effects of Heterobasidion annosum s.s. infection on severity of Diplodia pinea tip blight in Italian stone pine (Pinus pinea L.). Tree Physiology 28, 1653–1660. Wallis, C.M., Eyles, A., Chorbadjian, R., McSpadden-Gardner, B.B., Hansen, R., Cipollini, D.F., Herms, D.A. and P. Bonello. 2008. Systemic induction of phloem secondary metabolism and its relationship to resistance to a canker pathogen in Austrian pine. New Phytologist 177, 767–778. Luchi, N., P. Capretti, and P. Bonello. 2007. Production of Diplodia scrobiculata and Diplodia pinea pycnidia on ground Austrian pine needle agar medium. Phytopathologia Mediterranea 46, 230-235. Eyles, A., R. Chorbadjian, C.M. Wallis, R.C. Hansen, D.F. Cipollini, D.A. Herms, and P. Bonello. 2007. Cross- induction of systemic induced resistance between an insect and a fungal pathogen in Austrian pine over a fertility gradient. Oecologia 153: 365-374. Whitehill, J., J. S. Lehman, and P. Bonello. 2007. Ips pini (Curculionidae: Scolytinae) is a vector of the fungal pathogen, Sphaeropsis sapinea (Coelomycetes), to Austrian pines, Pinus nigra (Pinaceae). Environmental Entomology 36:114-120. Blodgett, J. T., A. Eyles, and P. Bonello. 2007. Organ-dependent induction of systemic resistance and systemic susceptibility in Pinus nigra inoculated with Sphaeropsis sapinea and Diplodia scrobiculata. Tree Physiology 27:511-517. Bonello, P., T. R. Gordon, D. A. Herms, D. L. Wood, and N. Erbilgin. 2006. Nature and ecological implications of pathogen-induced systemic resistance in conifers: A novel hypothesis. Physiological and Molecular Plant Pathology 68: 95-104. (Invited review article.) Wang, D., A. Eyles, and P. Bonello. 2006. Systemic aspects of host-pathogen interactions in Austrian pine (Pinus nigra): a proteomics approach. Physiological and Molecular Plant Pathology 68:149-157. Blodgett, J. T., D. A. Herms, and P. Bonello. 2005. Fertilization effects on red pine defense chemistry and resistance to Sphaeropsis sapinea. Forest Ecology and Management 208: 373-382. Luchi, N., R. Ma, P. Capretti, and P. Bonello. 2005. Systemic induction of traumatic resin ducts and resin flow in Austrian pine by wounding and inoculation with Sphaeropsis sapinea and Diplodia scrobiculata. Planta 221: 75-84. D. Synergistic Activities • Developed a hands-on workshop for high school teachers on “Chemical Ecology – Interactions Between Trees and Pathogens”. • Developed a hands-on class for third graders on “My career as a tree ecologist”. • Developed two new courses at OSU: “Advanced Topics in Fungal Biology”; “Fungi in Natural Ecosystems”, while a third was co-developed with Dr. McSpadden Gardener: “Ecology of Plant-Associated Microbes”. • Associate Editor for Plant Disease (Jan. 2001-Dec. 2003); Editorial Board for Physiological and Molecular Plant Pathology (2007-2010); ad hoc reviewer for Annals of Forest Science, Australasian Plant Pathology, Canadian Journal of Botany, Canadian Journal of Forest Research, Forest Ecology and Management, Forest Pathology, Forest Science, Journal of Chemical Ecology, Journal of Experimental Botany, Naturwissenschaften, Molecular Plant Pathology, Mycologia, Mycological Research, Mycorrhiza, Oecologia, Phytoparasitica, Phytopathology, Physiological and Molecular Plant Pathology, Planta, Plant Health Progress, Scandinavian Journal of Forest Research, Theoretical and Applied Genetics, Tree Physiology, Tropical Agriculture. • Official Ohio representative to Multistate Research Projects: W1187, “Interactions Among Bark Beetles, Pathogens, and Conifers in North American Forests” (immediate past chair), and NCR193, “IPM Strategies for Arthropod Pests and Diseases in Nurseries and Landscapes”.

E. Collaborators & Other Affiliations (i) Collaborators and Co-Editors (last 48 months): J.T. Blodgett – US Forest Service; M.J. Boehm - Ohio State University; P. Capretti – University of Florence, Italy; D. Cipollini – Wright State University; N. Erbilgin – University of California, Berkeley; A. Eyles – CSIRO; M. Garbelotto - University of California, Berkeley; T.R. Gordon – University of California, Davis; T.L. Graham - Ohio State University; R.C. Hansen - Ohio State University; D.A Herms - Ohio State University; D. Karnoski - Michigan Technological University; N. Kleczewski - Ohio State University; J.H. LaForest – Univ. of Georgia, Tifton, GA; C. Liang – Miami University; P.E. Lipps - Ohio State University; N. Luchi – University of Florence, Italy; R. Ma – unattached; W. Mattson – US Forest Service; B. McPherson – University of California, Berkeley; B.B. McSpadden Gardener – Ohio State University; M. Michelozzi, CNR, Florence, Italy; M. Mielke – US Forest Service; A. Nagle - Ohio State University; F. Ockels – Water Quality Dept., City of Dallas, TX; K. Raffa – University of Wisconsin, Madison; E. Rebek – Oklahoma State University; D.M. Rizzo – University of California, Davis; D. Smitley - Michigan State University; P. Svihra – private consultant; N. Taylor - Ohio State University; S.L. Thomas – California Dept. of Food and Agriculture; C. Wallis – University of Northern British Columbia; J. Whitehill - Ohio State University; D.L. Wood – University of California, Berkeley.

(ii) Graduate and Postdoctoral Advisors: R.B. Pearce (deceased) - University of Oxford, UK, Ph.D. advisor; W. Heller, GSF - Forschungszentrum für Umwelt und Gesundheit, Munich, Germany, postdoctoral sponsor; T.D. Bruns - University of California, Berkeley, postdoctoral sponsor; T.R. Gordon - University of California, Davis, postdoctoral sponsor; D.L. Wood - University of California, Berkeley, postdoctoral sponsor.

(iii) Thesis Advisor and Postgraduate-Scholar Sponsor: Post-docs A. Eyles (completed); J.T. Blodgett (completed).

Graduate Students Annemarie Nagle – M.S. (current); Justin Whitehill – Ph.D. (current); Nathan Kleczewski - Ph.D. (current); Brian Goldberger – Ph.D. (current); Christopher Wallis - Ph.D. (completed); France Ockels – M.S. (completed); Joe LaForest – M.S. (completed); Rui Ma – M.S. (completed). Principal Investigator/Program Director (Last, First, Middle): Ding, Biao

BIOGRAPHICAL SKETCH Provide the following information for the key personnel in the order listed on Form Page 2. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Biao Ding Professor of Plant Cellular and Molecular Biology

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) Beijing Forestry University B.S. 1982 Forestry Cornell University M.S. 1986 Plant Anatomy Cornell University Ph.D. 1991 Plant Cell Biology University of California, Davis Postdoc 1991-1994 Plant Cell Biology

A. Positions and Honors

Professional Positions 1994-1999 Assistant Professor, Department of Botany, Oklahoma State University, OK 1999-2000 Associate Professor, Department of Botany, Oklahoma State University, OK 2000-2005 Associate Professor, Department of Plant Cellular and Molecular Biology and Plant Biotechnology Center, Ohio State University, OH 2005- Professor, Department of Plant Cellular and Molecular Biology and Plant Biotechnology Center, Ohio State University, OH

Other Experience and Professional Memberships Member, American Society for Virology Member, American Society for Microbiology Member, International Society of Molecular Plant-Microbe Interactions Member, American Society of Plant Biologists

Honors 1983-1985 Graduate Fellowship for Overseas Studies, the Chinese Government 1999 The College of Arts and Sciences Faculty Council Junior Faculty Award for Scholarly Excellence, Oklahoma State University 2005 Harlan Hatcher Memorial Award for Excellence, Colleges of Arts and Sciences, Ohio State University (2005) 2005-2007 Outstanding Overseas Youth Research Award, National Science Foundation of China

B. Selected Peer-Reviewed Publications (Selected from 42 peer-reviewed research papers)

1. Ding, B., Haudenshield, J.S., Hull, R.J., Wolf, S., Beachy, R.N., and Lucas, W.J. (1992). Secondary plasmodesmata are specific sites of localization of the tobacco mosaic virus movement protein in transgenic tobacco plants. Plant Cell 4:915-928.

PHS 398/2590 (Rev. 05/01) Page 5 Biographical Sketch Format Page Principal Investigator/Program Director (Last, First, Middle): 2. Ding, B., Haudenshield, J.S., Willmitzer, L., and Lucas, W.J. (1993). Correlation between arrested secondary plasmodesmal development and onset of accelerated leaf senescence in yeast invertase transgenic tobacco plants. Plant J. 4:179-189. 3. Fujiwara, T., Giesmann-Cookmeyer, D., Ding, B., Lommel, S.A., and Lucas, W.J. (1993). Cell-to-cell trafficking of macromolecules through plasmodesmata potentiated by the red clover necrotic mosaic virus movement protein. Plant Cell 5:1783-1794. 4. Sanger, M., Passmore, B., Falk, B.W., Bruening, G., Ding, B., and Lucas, W.J. (1994). Symptom severity of beet western yellows virus strain ST9 is conferred by the ST9-associated RNA and is not associated with virus release from the phloem. Virology 200:48-55. 5. Lucas, W.J., Bouche-Pillon, S., Jackson, D.P., Nguyen, L., Baker, L., Ding, B. and Hake, S. (1995). Selective trafficking of KNOTTED1 homeodomain protein and its mRNA through plasmodesmata. Science 270:1980-1983. 6. Ding, B., Li, Q-b., Nguyen, L., Palukaitis, P., and Lucas, W.J. (1995). Cucumber mosaic virus 3a protein potentiates cell-to-cell trafficking of CMV-vRNA in tobacco plants. Virology 207:345-353. 7. Nguyen, L., Lucas, W. J., Ding, B. and Zaitlin, M. (1996). Viral trafficking is inhibited in replicase- mediated resistant transgenic tobacco plants. Proc. Natl. Acad. Sci. (U.S.A.) 93:12643-12647. 8. Ding, B., Kwon, M.-O., and Warnberg, L. (1996). Evidence that actin filaments are involved in controlling the permeability of plasmodesmata in tobacco mesophyll. Plant J. 10:157-164. 9. Itaya, A., Hickman, H., Bao, Y., Nelson, R. and Ding, B. (1997). Cell-to-cell trafficking of cucumber mosaic virus movement protein:green fluorescent protein fusion produced by biolistic gene bombardment in tobacco. Plant J. 12:1223-1230. 10. Ding, B., Kwon, M.-O., Hammond, R. and Owens, R. (1997). Cell-to-cell movement of potato spindle tuber viroid. Plant J. 12:931-936. 11. Itaya, A., Woo, Y.-M., Masuta, C., Bao, Y., Nelson, R., and Ding, B. (1998). Developmental regulation of intercellular protein trafficking through plasmodesmata in tobacco leaf epidermis. Plant Physiol. 118:373-385. 12. Woo, Y.-M., Itaya, A., Owens, R.A., Tang, L., Hammond, R.W., Chou, H.-C., Lai, M.M.C., and Ding, B. (1999). Characterization of nuclear import of potato spindle tuber viroid RNA in permeabilized protoplasts. Plant J. 17:627-635. 13. Itaya, A., Liang, G., Nelson, R.S., and Ding, B. (2000). Nonspecific intercellular protein trafficking probed by green fluorescent protein. Protoplasma 213:165-175. 14. Zhu, Y., Green, L., Woo, Y.-M., Owens, R., and Ding, B. (2001). Cellular basis of Potato spindle tuber viroid systemic movement. Virology 279:69-77. 15. Itaya, A., Folimonov, A., Matsuda, Y., Nelson, R., and Ding, B. (2001). Potato spindle tuber viroid as inducer of RNA silencing in infected tomato. Molec. Plant-Microbe Interact. 14:1332-1334. 16. Owens, R.A., Blackburn, M. and Ding, B. (2001). Possible involvement of phloem protein 2 in long distance viroid movement. Molec. Plant-Microbe Interact. 14:905-909. 17. Qi, Y. and Ding, B. (2002) Replication of Potato spindle tuber viroid in cultured cells of tobacco and Nicotiana benthamiana: The role of specific nucleotides in determining replication levels for host adaptation. Virology 302:445-456. 18. Itaya, A., Ma, F., Qi, Y., Matsuda, Y., Zhu, Y., Liang, G., and Ding, B. (2002). Plasmodesma-mediated selective protein traffic between “symplasmically-isolated” cells probed by a viral movement protein. Plant Cell 14: 2071-2083. 19. Zhu, Y., Qi, Y., Xun, Y., Owens, R., and Ding, B. (2002) Movement of Potato spindle tuber viroid reveals regulatory points of phloem-mediated RNA traffic. Plant Physiol. 130:138-146. 20. Itaya, A., Matsuda, Y., Gonzales, R. A., Nelson, R.S., and Ding, B. (2002) Potato spindle tuber viroid strains of different pathogenicity induces and suppresses expression of common and unique genes in infected tomato. Molec. Plant-Microbe Interact. 15:990-999.

PHS 398/2590 (Rev. 05/01) Page Continuation Format Page Principal Investigator/Program Director (Last, First, Middle):

21. Matsuda, Y., Liang, G., Zhu, Y., Ma, F., Nelson, R.S., and Ding, B. (2002). The Commelina yellow mottle virus promoter drives companion cell-specific gene expression in multiple organs of transgenic tobacco. Protoplasma 220:51-58. 22. Qi, Y. and Ding, B. (2003). Differential subnuclear localization of RNA strands of opposite polarity derived from an autonomously-replicating viroid. Plant Cell 15:2566-2577. 23. Qi, Y. and Ding, B. (2003). Inhibition of cell growth and shoot development by a specific nucleotide sequence in a noncoding viroid RNA. Plant Cell 15:1360-1374. 24. Qi, Y., Pélissier, T., Itaya, A., Hunt, E., Wassenegger, M., and Ding, B. (2004). Direct role of a viroid RNA motif in mediating directional RNA trafficking across a specific cellular boundary. Plant Cell 16:1741-1752. 25. Qi, Y., Zhong, X., Itaya, A., and Ding, B. (2004). Dissecting RNA silencing in protoplasts uncovers novel effects of viral suppressors on the silencing pathway at the cellular level. Nucleic Acids Research 32 (22): e179. 26. Cao, X., Zhou, P., Zhang, X., Zhu, S., Zhong, X., Xiao, Q., Ding, B., Li, Y. (2005). Identification of an RNA silencing suppressor from a plant double-stranded RNA virus. J. Virol. 79:13018-13027. (Selected by Editor for Spotlight) 27. Zhong, X., Leontis, N. B., Qian, S., Itaya, A., Boris-Lawrie, K., and Ding, B. (2006). Tertiary structural and functional analyses of a viroid RNA motif by isostericity matrix and mutagenesis reveal its essential role in replication. J. Virol. 80:8566-8581. 28. Itaya, A., Zhong, X., Bundschuh, R., Qi, Y., Wang, Y., Takeda, R., Harris, A.R., Molina, C., Nelson, R.S., and Ding, B. (2007). A structured viroid RNA is substrate for dicer-like cleavage to produce biologically active small RNAs but is resistant to RISC-mediated degradation. J. Virol. 81:2980-2994 (Highlighted by Faculty 1000 Biology as “Must Read” on April 16, 2007) 29. Wang, Y., Zhong, X., Itaya, A., and Ding, B. (2007). Evidence for the in vivo existence of loop E motif of potato spindle tuber viroid. J. Virol. 81:2074-2077. 30. Zhong, X., Tao, X., Stombaugh, J., Leontis, N., and Ding, B. (2007). Tertiary structure and function of an RNA motif required for plant vascular entry to initiate systemic trafficking. EMBO J. 26:3836-3846. 31. Zhou, F., Pu, Y., Wei, T., Liu, H., Deng, W., Wei, C., Ding, B., Omura, T., Li, Y. (2007) The P2 capsid protein of the nonenveloped rice dwarf phytoreovirus induces membrane fusion in insect host cells. Proc. Natl. Acad. Sci. USA 104:19547-19552. 32. Itaya, A., Bundschuh, R., Archual, A., Joung, J.-G., Fei, Z., Dai, X., Zhao, P., Tang, Y., Nelson, R.S. and Ding, B. (2007). Small RNAs in tomato fruit and leaf development. Biochim Biophy Acta 1779:99-107. 33. Zhong, X., Archual, A.J., Amin, A.A., and Ding, B. (2008) A genomic map of viroid RNA motifs critical for replication and systemic trafficking. Plant Cell 20:35-47. (Highlighted as Editor’s Choice in the February 8, 2008 issue of Science.)

C. Research Support

Ongoing Research Support NSF IBN-0238412 Ding (PI) 8/1/2006-7/31/2009 An integrative approach to elucidate RNA replication and systemic trafficking This study investigates the RNA structural motifs and cellular factors critical for replication as well as RNA structural motifs essential for intercellular trafficking, using potato spindle tuber viroid as the model. Role: PI

Completed Research Support

USDA NRICGP 2004-35304-15005 Ding (PI) 9/1/2004-8/31/2008 Biochemical and genetic analyses of plant microRNA biogenesis This project investigates how plant microRNAs are produced. Role: PI

PHS 398/2590 (Rev. 05/01) Page Continuation Format Page Principal Investigator/Program Director (Last, First, Middle):

NSF IBN-0238412 Ding (PI) 3/15/2003-3/14/2006 Mechanisms of phloem-mediated RNA traffic This study investigates mechanisms that control phloem-mediated long-distance RNA traffic, using potato spindle tuber viroid as the model. Role: PI

NSF IOB-0515745 Ding (PI) 3/1/2005-2/28/2007 Novel biogenesis and function of small RNAs derived from a plant pathogen This project studies the biogenesis and function of viroid-derived small RNAs. Role: PI

USDA NRICGP 2002-35304-12272 Ding (PI) 8/15/2002-8/14/2004 Cellular and genetic analysis of intercellular protein traffic The goal of this study was to investigate cellular boundaries that regulate intercellular protein trafficking and develop genetic screening systems of Arabidopsis mutants defective in protein trafficking. Role: PI

The Samuel Roberts Noble Foundation Ding (PI) Nelson (CoPI) 1/1/2000-11/1/2003 Plant development and viral movement This project examined how plant development affects viral infection patterns. Role: PI

The Ohio Plant Biotechnology Consortium Ding (PI) 7/1/2004-6/30/2005 Development of novel microRNAs for gene function studies and for viral resistance This projects tests the idea of engineering vectors to express designer microRNAs for gene regulation and anti-viral infection. Role: PI

USDA NRICGP 2001-35304-09928 Ding (PI) 12/1/2000-11/30/2002 Intercellular protein trafficking and leaf development This project investigated the role of leaf developmental stages in controlling intercellular protein trafficking patterns. Role: PI

PHS 398/2590 (Rev. 05/01) Page Continuation Format Page JOHN J. FINER Department of Horticulture and Crop Science, Plant Molecular Biology and Biotechnology Program, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Ave, Wooster, Ohio 44691, USA, http://www.oardc.ohio-state.edu/plantranslab/ Tel: 330-263-3880, Fax: 330-263-3887, E-mail - [email protected]

PROFESSIONAL PREPARATION

Miami University, Oxford, OH Botany BS 1978 Texas A&M University Plant Physiology MS 1981 Texas A&M University Plant Physiology PhD 1984 Postdoctoral Fellow CIBA-GEIGY Biotechnology 1984-1986 APPOINTMENTS 1999-present Professor, Department of Horticulture and Crop Science, Plant Molecular Biology and Biotechnology Program, The Ohio State University 1991-1999 Associate Professor, Department of Horticulture and Crop Science, The Ohio State University 1986-1991 Assistant Professor, Department of Agronomy, The Ohio State University

PUBLICATIONS

Chiera JM, Lindbo JA, Finer JJ (in press) Quantification and extension of transient GFP expression by the co-introduction of a suppressor of silencing. Transgenic Research, DOI: 10.1007/s11248-008-9192-5 Wu C, Chiera JM, Ling PP, Finer JJ (in press) Isoxaflutole treatment leads to reversible tissue bleaching and allows for more effective detection of GFP in transgenic soybean tissues. In Vitro Cellular and Developmental Biology – Plant, DOI: 10.1007/s11627-008-9126-1 Finer JJ, Larkin KM (in press) In (ed. PB Kirti) Handbook of New Technologies for Genetic Improvement of Legumes, “Genetic transformation of soybean using particle bombardment and SAAT approaches” Haworth Press, Binghamton, New York Finer JJ, Dhillon T (2008) Transgenic Plant Production, pp 245-272 In: Plant Biotechnology and Genetics: Principles, Techniques and Applications, ed. C. Neal Stewart, Jr. Wiley and Sons, New York Finer JE, Finer JJ (2007) A simple method for reducing moisture condensation on Petri dish lids. Plant Cell Tissue and Organ Culture 91:299-304 Chiera JM, Bouchard RA, Dorsey SL, Park EH, Buenrostro-Nava MT, Ling PP, Finer JJ (2007) Isolation of two highly active soybean (Glycine max (L.) Merr.) promoters and their characterization using a new automated image collection and analysis system. Plant Cell Reports 26:1501-1509 Chiera JM, Streeter JG, Finer JJ (2006) Ononitol and pinitol production in transgenic soybean containing the inositol methyl transferase gene from Mesembryanthemim crystallinum. Plant Science 171:647–654 Buenrostro-Nava MT, Ling PP, Finer JJ (2006) Comparative analysis of 35S and Lectin promoters in transgenic soybean tissue using an automated image acquisition system and image analysis. Plant Cell Reports 25:920-926 Finer JJ, Beck SL, Buenrostro-Nava MT, Chi YT, Ling PP (2006) In (Eds. S Dutta Gupta, Y Ibaraki) Plant Tissue Culture Engineering; Focus in Biotechnology, “Monitoring Gene Expression in Plant Tissues; Using green fluorescent protein with automated image collection and analysis” p 31-46, Springer, Dordrecht Buenrostro-Nava MT, Ling PP, Finer JJ (2005) Development of an automated image acquisition system for monitoring gene expression and tissue growth. Transactions of the Amer Soc for Agric Eng 48:841-847

SYNERGISTIC ACTIVITIES Served in the Society for In Vitro Biology, as secretary, vice-chair, chair, and development officer of the Plant Division. Currently serving as Secretary and Member of the Executive Board of the society. Developed new particle bombardment instrumentation that greatly expanded the use of this technology in smaller US laboratories and in developing countries. Developed robotics and gene tracking system, allowing simple quantification of gene expression data. Isolation and characterization of soybean promoters using automated image analysis and gfp. Developed curriculum materials for Plant Cell Transformation (graduate) and Ethics in Biotechnology (undergraduate) courses. Served as an electronics expert in biotechnology with COSI (Center of Science and Industry) in Columbus, OH for 7 years. COSI is a science center for K-12 students. Developed a seed germination exercise kit (Adolescent Seeds; Embryo Germination Before their Time) for middle and high school science students. COLLABORATORS AND OTHER AFFILIATIONS i. Collaborators Randy Dinkins (USDA/ARS), Elisabeth Grabau (Virginia Tech), Steve Knapp (University of Georgia), John Lindbo (University of California, Davis), EuiHo Park (Yeungnam University, Korea), Wayne Parrott (Univ of Georgia), Paul Rushton (University of Virginia), Harold Trick (Kansas State Univ), Lila Vodkin (Univ of Illinois), Jack Widholm (Univ of Illinois), Adriana Rodriguez (University of Sao Paulo), Mike Timko (University of Virginia). ii. Graduate and Postdoctoral Advisors Graduate Advisor: Roberta Smith, Texas A&M University (retired) Postdoctoral Advisor: Mary-Dell Chilton (Syngenta) iii. Thesis and Postgraduate-Scholar Sponsor Robert Bouchard (The Ohio State University), Aubry Brzozowski (BallHelix), Marco Buenrostro-Nava (Texas A&M University), Tai-Sheng Cheng (Tainan Teachers College, Tainan, Taiwan), Taniya Dhillon (The Ohio State University), Summer Dorsey (DuPont Agriculture and Nutrition), Masood Hadi (Laurence Livermore Research Labs), Carlos Hernandez-Garcia (The Ohio State University), Kathryn Larkin (Arizona State University), Marcelo Pomeranz (The Ohio State University), Eliane Santarem (Pontifícia Universidade Católica do Rio Grande do Sul), Harold Trick (Kansas State University), Sanchita Vaghchhipawala (Noble Foundation), Philippe Vain (John Innes Research Center, UK), Nicole Waterland (The Ohio State University), Congling Wu (University of Georgia) Total number of graduate students advised: 11 Total number of postdoctoral scholars advised: 10 David M. Francis Department of Horticulture and Crop Science, The Ohio State University-Ohio Agricultural Research and Development Center, Wooster, OH 44691 Tel: (330) 263-3893; FAX: 330-263-3887; E-mail: [email protected]

Professional Preparation Pomona College, Claremont, CA B.A. 1984 Biology University of California, Davis, CA Ph.D 1991 Genetics University of California, Davis, CA Post-Doc 1991-1995 Genetics

Appointments (2003-present) Associate Professor, Department of Horticulture and Crop Science, OARDC/The Ohio State University, Wooster OH 44691. (1999-2003) Asistant Professor, Department of Horticulture and Crop Science, OARDC/The Ohio State University, Wooster OH 44691. (1995-1999) Research Scientist and Adjunct Assistant Professor, Department of Horticulture and Crop Science, OARDC/The Ohio State University, Wooster OH 44691. (1991-1999) Post-doctoral associate at the Department of Vegetable Crops, University of California, Davis, CA 95616. (1985-1991) McKnight Foundation Fellowship for interdisciplinary studies in plant/pathogen interactions, Univ. of CA, Davis, CA 95616.

Publications (last five years) Bentley, S. D., C. Corton, A. Barron, L. Clark, J. Doggett, B. Harris, D. Ormond, M. A. Quail, S. E. Brown, D. Knudson, D. Francis, J. Parkhill, C. Ishimaru. 2008. Genome of the actinomycete plant pathogen Clavibacter michiganensis subspecies sepedonicus suggests recent niche adaptation. J. Bact. 190:2150-2160 Darrigues, A., J. Hall, N. Dujmovic, S. Gray, E. van der Knaap, and D. M. Francis. 2008. Tomato Analyzer – Color Test: a new tool for efficient digital phenotyping. J. Amer. Soc. Hort. Sci. 133(4):579–586. Darrigues, A., S. Schwartz, D. M. Francis. 2007. Optimizing sampling of tomato fruit for carotenoid content with application to assessing the impact of ripening disorders. Journal of Agricultural and Food Chemistry 56: 483–487 Van Deynze, A., K. Stoffel, C. R. Buell, A. Kozik, J. Liu, E. van der Knaap, D. M. Francis. 2007. Diversity in conserved genes in tomato. BMC Genomics 8:465. http://www.biomedcentral.com/1471-2164/8/465 Unlu, N. Z., T. Bohn, D. Francis, S. J. Clinton, S. J. Schwartz. 2007. Carotenoid Absorption in Humans Consuming Pasta Sauces Obtained from Tangerine or High-B-Carotene Varieties of Tomatoes. Journal of Agricultural and Food Chemistry. 55(4):1597-1603. Unlu, N. Z., Bohn, T., Francis, D. M., Nagaraja, H. N., Clinton, S. K., and Schwartz, S. J. 2007. Lycopene from heat-induced cis-isomer-rich tomato sauce is more bioavailable than from all- trans-rich tomato sauce in human subjects. Br. J. Nutr. 98: 140-146 Halim, Y., S. Schwartz, D. Francis, N. A. Baldauf, and L. Rodriguez-Saona. 2006. Direct Determination of Lycopene Content in Tomatoes (Lycopersicon esculentum) by Attenuated Total Reflectance Infrared (ATR-IR) Spectroscopy and Multivariate Analysis. Journal of AOAC International. 89:1257-1262. Yang, W. and D. M. Francis. 2005. Marker Assisted Selection for Combining Resistance to Bacterial Spot and Bacterial Speck in Tomato. J. Amer. Soc. Hort. Sci. 130:716-721. Stommel, John R. Judith A. Abbott, T. Austin Campbell, and David Francis 2005. Inheritance of Elastic and Viscoelastic Components of Tomato Firmness Derived from Intra- and Interspecific Genetic Backgrounds J. Amer. Soc. Hort. Sci. 130: 598-604. Yang, W., E. J. Sacks, M. L. Lewis Ivey, S. A. Miller, and D. M. Francis. 2005. Resistance in Lycopersicon esculentum intraspecific crosses to Race T1 strains of Xanthomonas campestris pv. vesicatoria causing bacterial spot of tomato. Phytopathology 95:519-527. Francis, D. M. and S. A. Miller. 2005. Ohio 9834 and Ohio 9816: processing tomato breeding lines with partial resistance to race T1 of bacterial spot. HortScience 40: 1566-1568. Hackett, M.M., Lee, J.H., Francis, D. and Schwartz, S. 2004. Thermal stability and isomerization of lycopene in tomato oleoresins from different varieties. J. Food Sci., 69(7) 536-541. Coaker G., B. Willard, M. Kinter, E. J. Stockinger, and D. Francis. 2004. Proteomic analysis of resistance to bacterial canker of tomato. Molecular Plant Microbe Interactions. 17:1019-1028. Coaker G., and D. Francis. 2004. Mapping, genetic effects, and epistatic interaction of two bacterial canker resistance QTLs from Lycopersicon hirsutum. Theor Appl Genet. 108:1047-1055 Yang, W., X. Bai, E. Kabelka, C. Eaton, S. Kamoun, E. van der Knaap, and D. Francis. 2004. Discovery of single nucleotide polymorphisms in Lycopersicon esculentum by computer aided analysis of expressed sequence tags. Molecular Breeding. 14: 21-34. Kabelka, E., W. Yang, and D. M. Francis. 2004. Improved Tomato Fruit Color within an Inbred Backcross Line Derived from Lycopersicon esculentum and L. hirsutum Involves the Interaction of Loci. J. Amer. Soc. Hort. Sci. 129:250-257 Huitema, E., V. G. A. A. Vleeshouwers, D.M. Francis and S.Kamoun. 2003. Active defense responses associated with nonhost resistance of Arabidopsis thaliana to the oomycete pathogen Phytophthora infestans. Molecular Plant Pathology. 4: 487-500. Scott, J. W., D. M. Francis, S. A. Miller, G. C. Somodi, J. B. Jones. 2003. Tomato bacterial spot resistance derived from PI 114490; inheritance to race T2 and relationship across three pathogen races. J. Amer. Soc. Hort. Sci. 128:698-703. Qu, S., G. Coaker, D. Francis, B. Zhou, and G.L. Wang. 2003. Development of a new transformation- competent artificial chromosome (TAC) vector and construction of tomato and rice TAC libraries. Molecular Breeding. 12: 297-308. Burnham, K.D., A.E. Dorrance, D.M. Francis, R.J. Fioritto, and S.K. St. Martin. 2003. Rps8, A New Locus in Soybean for Resistance to Phytophthora sojae Crop Science 43: 101-105. Huitema, E., V. G. A. A. Vleeshouwers, D.M. Francis and S.Kamoun. 2003. Active defense responses associated with nonhost resistance of Arabidopsis thaliana to the oomycete pathogen Phytophthora infestans. Molecular Plant Pathology. 4: 487-500.

Synergistic Activities Member of the National Plant Germplasm System (NPGS) Crop Germplasm Committee (CGC) for tomato (2001-Present). Chair of the NPGS CGC for tomato (2005-present). Ohio Representative to the United States Department of Agriculture (USDA) North Central Region 7 (NC7) Germplasm Committee (2000-present). Secretary for (NC7) Germplasm Committee (2002-2004). Panel Member, joint USDA-DOE feedstock genomics, Rockville, MD, April 2006. Member of the International Scientific Committee for the 8 th International Symposium on the Processing Tomato and the 5th World Congress on the Processing Tomato (2002), Istanbul, Turkey; member of the International Scientific Committee for the 9 th International Symposium on the Processing Tomato and the 6th World Congress on the Processing Tomato (2004), Melbourne, Austalia. Co-Coordinator of the common use DNA Marker Laboratory in the Department of Horticulture and Crop Science; Molecular and Cellular Imaging Center Advisory Committee, OSU-OARDC, Wooster OH 44691; 1999-present.

Graduate and Postdoctoral Advisors R. W. Michelmore (Ph.D advisor, Univ. of California, Davis) D. A. St. Clair (Post-doctoral advisor, Univ. of California, Davis) Thesis Advisor and Postgraduate-Scholar Sponsor

Thesis advisor for: Audrey Darrigues, PhD student (2004-present) Susana de Jesus, MS 2005, Natural Products Chemist, Instituto de Inovacion en Biotecnologia e Industria (IIBI), Santo Domingo, Dominican Republic Gitta Coaker, PhD 2003, Currently Assistant Professor, University of California, Dept. of Plant Pathology, Davis, CA. Eileen Kabelka, Graduate Student, PhD 2001, Currently Ass. Prof. University of Florida, Gainsville, FL.

Postgraduate advisor for: Matt Robbins, Post-doctoral researcher (2006-present). Sung-Chur Sim, Post-doctoral researcher (2006-present). Alba McIntyre, Post-doctoral researcher (2002-2006). Currently Assistant Professor, Wayne College, University of Akron, OH. Wencai Yang, Post-doctoral researcher (2001-2005). Currently Assistant Professor, Dept. of Vegetable Science, China Agricultural University, Bejing, China. Kara Burnham, Post-doctoral researcher (1999-2002). Currently Assistant Professor, Dept. of Biology, Pacific University, Forest Grove, Oregon. Frank Dailey, Post-doctoral researcher (2002). Currently Assistant Professor, Microbiology and Medical Pathology, Lake Erie College School of Pharmacy, Erie, PA. Tea Meulia, Post-doctoral researcher (1999). Currently Department Head of the Molecular and Cellular Imaging Center, Wooster, OH. Erik Sacks, Post-doctoral researcher (1997-1998). Currently Research Geneticist, USDA, Stoneville, MS. BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Venkat Gopalan Associate Professor of Biochemistry eRA COMMONS USER NAME GOPALAN05 EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) University of Madras, Madras, INDIA B.Sc. 1986 Chemistry University of New Mexico, Albuquerque, NM Ph.D. 1991 Biochemistry Yale University, New Haven, CT Postdoc. 1992-1997 Molecular biology

A. Positions and Honors Academic Appointments 1986-1991 Doctoral studies with Robert H. Glew, Ph.D., Dept. of Biochemistry, University of Pitts- burgh, Pittsburgh, PA and University of New Mexico, Albuquerque, NM 1992-1997 Postdoctoral training with Sidney Altman, Ph.D., Dept. of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 1998-2003 Assistant Professor, Dept. of Biochemistry, The Ohio State University, Columbus, OH 2000-present Adjunct Faculty, Dept. of Plant Biology, The Ohio State University, Columbus, OH 2003-present Associate Professor, Dept. of Biochemistry, The Ohio State University, Columbus, OH

Selected Professional Activities 1998-present Reviewer: BBA, Biochemistry, Genome Biology, Journal of Biological Chemistry, Journal of Molecular Biology, Molecular BioSystems, Molecular Microbiology, Mutation Research, Nucleic Acids Research, Oligonucleotides, PNAS, RNA, and Science. 1999-present Ad hoc grant reviewer for: Petroleum Research Fund, Wellcome Trust, and NSF 2003-2004 Panel Member, Biochemistry of Gene Expression Panel, NSF

Honors 1993-1995 Post-doctoral Fellow of the Donaghue Medical Research Foundation 1995-1996 Post-doctoral Fellow of the Anna Fuller Cancer Research Fund 2003 OSU College of Biological Sciences Dean’s Award for Undergraduate Research Mentoring 2005 OSU College of Biological Sciences Dean’s Award for Classroom Teaching 2007 OSU Department of Biochemistry Award for Excellence 2008 OSU Harlan Hatcher Memorial Award for Academic Excellence

B. Selected Publications (of 40) Gopalan, V., Daniels, L. B., Glew, R. H., and Claeyssens, M. (1989) Kinetic analysis of the interaction of akyl glycosides with two mammalian beta-glucosidases. Biochem. J. 262, 541-548. Glew, R. H., Gopalan, V., Hubbell, C. A., Beutler, E., Geil, J. D., and Lee, R. E. (1991) A case of non- neurologic Gaucher's disease that resembles the neurologic type biochemically. J. Neuropathol. Exp. Neu- rol. 50, 108-117 Gopalan, V., Vander Jagt, D. J., Libell, D. P., and Glew, R. H. (1992) Transglucosylation as a probe of the mechanism of action of mammalian cytosolic beta-glucosidase. J. Biol. Chem. 267, 9629-9638. Gopalan, V., Pastuszyn, A., Galey, W. R., Jr., and Glew, R. H. (1992) Exolytic hydrolysis of toxic plant glu- cosides by guinea pig liver cytosolic beta-glucosidase. J. Biol. Chem. 267, 14027-14032. Gopalan, V., Baxevanis, A., Landsman, D. and Altman, S. (1997) Analysis of the functional role of conserved residues in the protein subunit of ribonuclease P from Escherichia coli. J. Mol. Biol. 267, 818-829. Gopalan, V., Golbik, R., Schreiber, G., Fersht, A. and Altman, S. (1997) Fluorescence properties of a tryptophan residue in an aromatic core of the protein subunit of ribonuclease P from Escherichia coli. J. Mol. Biol. 267, 765-769. Gopalan, V., Kuhne, H., Biswas, R., Li, H., Brudvig, G. W. and Altman, S. (1999) Mapping RNA-protein interactions in ribonuclease P from Escherichia coli using electron paramagnetic resonance spectroscopy. Biochemistry 38, 1705-1714. Biswas, R., Ledman, D., Fox, R. O., Altman, S. and Gopalan, V. (2000) Mapping RNA-protein interactions in ribonuclease P from Escherichia coli using disulfide-linked EDTA-Fe. J. Mol. Biol. 296, 19-31. Stephen Raj, M. L., Pulukkunat, D. K., Reckard, J. F., Thomas, G. and Gopalan, V. (2001) Cleavage of bi- partite substrates by rice and maize RNase P: Application to targeted degradation of mRNAs in plants. Plant Physiol. 125, 1187-1190. Biswas, R., Kuhne, H., Brudvig, G. and Gopalan, V. (2001) Use of EPR spectroscopy to study macromo- lecular structure and function. Sci. Prog. 84, 45-67. Wu, C-W., Eder, P. S., Gopalan, V. and Behrman, E. J. (2001) Kinetics of coupling reactions that generate monothiophosphate disulfides: Implications for modification of RNAs. Bioconj. Chem. 12, 842-844. Gopalan, V., Vioque, A. and Altman, S. (2002) RNase P: variations and uses. J. Biol. Chem. 277, 6759- 6762. Eubank, T., Biswas, R., Jovanovic, M., Litovchick, A., Lapidot, A. and Gopalan, V. (2002) Inhibition of bacterial RNase P by modified aminoglycosides. FEBS Lett. 511, 107-112. Jovanovic, M., Sanchez, R., Altman, S. and Gopalan, V. (2002) Elucidation of structure-function relationships in the protein subunit of bacterial RNase P using a genetic complementation approach. Nucleic Ac- ids Res. 30, 5065-5073. Tsai, H-Y., Masquida, B., Biswas, R., Westhof, E. and Gopalan, V. (2003) Molecular modeling of the three- dimensional structure of the bacterial RNase P holoenzyme. J. Mol. Biol. 325, 661-675. Pulukkunat, D. K., Stephen Raj, M. L., Pattanayak, D., Lai, L. B. and Gopalan, V. (2003) Exploring the potential of plant RNase P as a functional genomics tool. In “Functional genomics: Methods and Protocols” (Grotewold, E., Ed.), pp. 295-309, Humana Press, Totowa, NJ. Eder, P. S., Hatfield, C., Vioque, A. and Gopalan, V. (2003) Bacterial RNase P as a potential target for novel anti-infectives. Curr. Op. Invest. Drugs 4, 937-943. Boomershine, W. P., Stephen Raj, M. L., Gopalan, V. and Foster, M. P. (2003) Preparation of uniformly la- beled NMR samples in Escherichia coli under the tight control of the araBAD promoter: Expression of an archaeal homolog of the RNase P Rpp29 protein. Prot. Expr. Purif. 28, 246-251. Boomershine, W. P., McElroy, C. A., Tsai, H. Y., Wilson, R. C., Gopalan, V. and Foster, M. P. (2003) Structure of Mth11/MthRpp29, an essential protein subunit of archaeal and eukaryal RNase P. Proc. Natl. Acad. Sci. USA 100, 15398-15403. Rangarajan, S., Raj, M. L. S., Hernandez, J. M., Grotewold, E. and Gopalan, V. (2004) RNase P-mediated disruption of gene expression in maize cells. Biochem. J. 380, 611-616. Behrman, E. J. and Gopalan, V. (2005) Cholesterol and plants. J. Chem. Ed. 82: 1791-1793. Tsai, H-Y., Pulukkunat, D. K., Woznick, W. and Gopalan, V. (2006) Functional reconstitution and characterization of Pyrococcus furiosus RNase P. Proc. Natl. Acad. Sci. USA 103, 16147-16152. Lai, L. B., Gopichandran, V., and Gopalan, V. (2006) Tangier disease: A disorder in the reverse cholesterol transport pathway. In “Clinical Studies in Medical Biochemistry”, (Glew, R. H. and Rosenthal, M. D., Eds.), pp. 159-166, Oxford University Press, New York, NY. Gopalan, V. and Altman, S. (2007) Ribonuclease P: structure and catalysis. In “The RNA World” (Geste- land, R. F., Cech, T. R. & Atkins, J. F., Eds.), Electronic version, CSH Laboratory Press, New York, NY. Gopalan, V. (2007) Uniformity amid diversity in RNase P. Proc. Natl. Acad. Sci. USA 104, 2031-2032. Behrman, E. J. and Gopalan, V. (2007) The anomeric specificity of enzymes which act on sugars. J. Chem. Ed. 84, 1608. Kawamoto, S. A., Sudhahar, C. G., Hatfield, C., Sun, J., Behrman, E. J., Gopalan, V. (2008) Studies on the mechanism of inhibition of bacterial RNase P by aminoglycosides. Nucleic Acids Res. 36, 697-704. Pulukkunat, D. K. and Gopalan, V. (2008) Studies on Methanocaldococcus jannaschii RNase P reveal insights into the roles of RNA and protein cofactors in RNase P catalysis. Nucleic Acids Res. 36, 4172-4180. Behrman, E. J. and Gopalan, V. (2008) Phosphoenolpyruvate: An end to handwaving. BAMBED 36, 323- 324. C. Research Support

Ongoing Research Support R01 GM067807 Foster, M. P. (PI) Gopalan, V. (Co-PI) 04/01/04-03/31/09 NIH/NIGMS Structure and function in catalytic RNP assembly The overall goal of this project is to use archaeal RNase P, a tRNA processing enzyme, as the model system to gain structural insights into how proteins modulate RNA catalysis in a ribonucleoprotein complex. Role: Co-PI

MCB-0238233 Gopalan, V. (PI) 04/01/03-09/30/08 NSF/CAREER AWARD Characterization of plant RNase P and examination of its utility as a functional genomics tool The goals of this project are (i) to identify and characterize the RNA and protein subunits of plant RNase P, and (ii) assess the feasibility of RNase P-mediated inhibition of gene expression in plants. Role: PI

Completed Research Support (partial list) MCB-0091081 Gopalan, V. (PI) 03/01/01-08/31/04 NSF RNA-protein interactions in bacterial ribonuclease P Our research objectives encompassed the use of multiple biochemical and biophysical approaches to elucidate the structural and mechanistic basis for the roles of the protein cofactor in Escherichia coli RNase P catalysis. Role: PI

DBI-0509744 Gopalan, V. (PI) 02/01/05-07/31/07 NSF Evaluating ribonuclease P and RNAi as tools for targeted RNA degradation in plants The overall objective was to compare the efficacy, target specificity and spatial control of the EGS method vs. RNAi in Arabidopsis. Role: PI Madge Y. (Lian-mei) Graham Curriculum Vitae

Senior Research Scientist Telephone: (614)-292-6828 Dept. of Plant Pathology Fax: (614)-292-4455 Ohio State University Email: [email protected] Columbus, Oh 43210

I. Professional Preparation: National Taiwan University, Agricultural Chemistry, B. S. Purdue University, Biochemistry, Ph.D.

II. Professional Experience/Appointments: 2005-present Senior staff research scientist, Dept. of Plant Pathology, Ohio State Univ. 2000-present Member, Plant Molecular Biology and Biotechnology program, Ohio State Univ. 1997-2004 Research Scientist, Department of Plant Pathology, Ohio State University 1991-1996 Senior Research Associate, Dept. of Plant Pathology and Ohio Agricultural Research and Development Center, Ohio State Univ. 1986-1990 Research Associate, Biotechnology Center and Dept. of Plant Pathology, Ohio State Univ. 1984-1985 Research Associate, Department of Pharmacology, Washington Univ. St. Louis. 1981-1983 Research Associate, Department of Genetics, Washington Univ. St. Louis. 1978-1980 NIH Post-Doctoral Trainee, Department of Microbiology and Immunology, Washington Univ. St. Louis 1975-1977 Post-Doctoral Researcher, Department of Pharmacology, U. of Wisconsin 1974-1975 Post-Doctoral Researcher, Department of Chemistry, Denver University

III. Research Interests: Soybean defense regulons: induced gene expression triggered by pathogen elicitors (pathogen associated molecular patterns or PAMPs) and chemicals causing cell death (as hypersensitive reaction mimics). These are studied by various methods/platforms for monitoring/profiling gene expression and via collaborative efforts of gene silencing for functional assignments in soybean.

IV. Publications: 5 selected recent papers: Graham TL, Graham MY, Yu O. 2008. Genomics of Soybean Secondary Product Metabolism. In: Genetics and Genomics of Soybean, Stacey G, ed. Springer Verlag, NY, pp. 241-242. Graham TL, Graham MY, Subramanian S, Yu O. 2007. RNAi silencing of genes for elicitation or biosynthesis of 5-deoxyisoflavonoids suppresses race specific resistance and hypersensitive cell death in Phytophthora sojae infected tissues. Plant Physiol. 144: 728-740. Graham MY. 2005. The Diphenylether Herbicide Lactofen Induces Cell Death and Expression of Defense-Related Genes in Soybean. Plant Physiol. 139: 1784-1794. Subramanian S, Graham MY, Yu O, Graham TL. 2005 RNA Interference of Soybean Isoflavone Synthase Genes Leads to Silencing in Tissues Distal to the Transformation Site and to Enhanced Susceptibility to Phytophthora sojae. Plant Physiol. 137:1345-1353 Graham MY, Weidner J, Wheeler K, Pelow ML, Graham TL. 2003. Pathogenesis-related protein gene induction in soybean by wounding and the Phytophthora sojae cell wall glucan elicitor. Physiol. Molec. Plant Pathol. 63:141-149 5 selected previous papers: Graham TL, Graham MY. 1999. Role of hypersensitive cell death in conditioning elicitation competency and defense potentiation. Physiol. Molec. Plant Pathol. 55:13-20. Graham TL, Graham MY. 1996. Signaling in soybean phenylpropanoid responses-dissection of primary, secondary, and conditioning effects of light, wounding, and elicitor treatments. Plant Physiology 110:1123-1133. Graham MY, Graham TL. 1994. Wound-associated Competence factors are required for the proximal cell responses of soybean to the Phytophthora-sojae wall glucan elicitor. Plant Physiology 105:571-578. Graham TL, Graham MY. 1991. Cellular Coordination of molecular responses in plant defense. Molecular Plant-Microbe Interact. 4:415-422. Olson MV, Dutchik JE Graham MY. et al. 1986. .Random-clone strategy for genomic restriction mapping in yeast. Proc. Nat. Acad. Sci. 83:7826-7830.

V. Synergistic Activities: a) Affiliated with IS-MPMI, APS, ASPB (also previously ASM), attend and present in 1-2 society and other meetings/conferences annually. b) Ad hoc reviewer for journals: Plant Physiology, Physiologia Plantarum, Plant Science, Physiological and Molecular Plant Pathology, and Canadian Journal of Botany. c) Ad hoc reviewer for grants from USDA and NSF. d) Attend and present in annual meetings of Ohio Plant Biotech Consortium and involved in state- wide scientific interactions. Participate in campus-wide information exchanges, particularly with students and postdocs (via seminar series, symposia and workshops in molecular plant sciences). These include the Plant Molecular Biology and Biotechnology (PMBB), Translational Plant Science (TPS), Molecular Plant Pathology and other programs.

VI. Collaborators and Other Affiliations: a) Collaborators (M.S. and Ph.D. level only): Graham, Terrence L., Department of Plant Pathology, Ohio State University Yu, Oliver, Donald Danforth Plant Science Center, St. Louis Submanian, Santhil, Donald Danforth Plant Science Center, St. Louis St. Martin, Steve, Department of Horticulture and Crop Science, Ohio State University b) Graduate and Post-doctoral Advisors (non-retired; with current affiliations): Olson, Maynard (Department of Genetics, University of Washington, Seattle) Boime, Irving (Department of Pharmacology, Washington University, St Louis) c) Activity for advising graduate and undergraduate students (within last 5 years): Thesis advising: Larue, Clayton: M.S. informal co-advising (currently: post-doctoral researcher, Univ. of Illinois) Cheng, Jiye: Ph. D. informal co-advising (current: Plant-Pathology and PMBB program) Outreach undergraduate training: Garrett, Katy: advising for undergraduate research competition (OSU Denman). Riggs, Kara: co-advising and providing some material. Terrence L. Graham Professor, Department of Plant Pathology Plant Molecular Biology and Biotechnology Program The Ohio State University

(i). Professional Preparation: B.S. Biochemistry, Suma cum laude, Pennsylvania State University M.S. Biochemistry, Purdue University Ph.D. Biochemistry, Purdue University Post-Doctoral Plant Pathology, University of Wisconsin (with Luis Sequeira, Academy of Science) (ii). Appointments: 2002- Professor, Department of Plant Pathology, The Ohio State University. 1996-2000 Associate Chair, Department of Plant Pathology, The Ohio State University 1992-2002 Associate Professor, Department of Plant Pathology, The Ohio State University. 1986-1992 Assistant Professor, Department of Plant Pathology, The Ohio State University. 1984-1986 Research Manager and Senior Science Fellow, Cellular and Molecular Biology, Monsanto Company 1982-1984 Science Fellow, Biological Control, Monsanto Company 1979-1982 Senior Research Group Leader, Stress Biochemistry Group, Monsanto Company 1977-1979 Project Leader, Host-Pathogen Project, Monsanto Company

(iii). Selected Honors & Other Recognition: Honorary Fraternities: Phi Eta Sigma, Phi Kappa Phi, Phi Lambda Upsilon (Chemistry Honorary) Charles Gerth Scholarships (Biochemical Scholastic), Pennsylvania State University. Evan Pugh Silver Medal (Upper 0.5% of Junior Class), Pennsylvania State University. Evan Pugh Gold Medal (Upper 0.5% of Senior Class), Pennsylvania State University. Summa Cum Laude Graduate of Pennsylvania State University. Class rank, 2nd, School of Science John White Graduate Fellowship, Pennsylvania State University. University Graduate Fellowship, Purdue University. Public Health Service National Research Service Awards for Post-Doctoral Training in Pathology Monsanto Achievement and Incentive Awards (3 years). Monsanto Science Fellow, Monsanto Senior Science Fellow College of Food, Agricultural and Environmental Sciences, Award for Excellence in Teaching The Ohio State University, Dept of Plant Pathology Research Award.

(iv) Selected Recent Professional Activities Over 20 invited talks at conferences, seminars and symposia since 2003, including a plenary talk at the Annual Meeting of the American Phytopathological Society in 2006. Organizer of over 5 Research Symposia since 2000, including the Session “Plant Cell Death” at the International Congress of Stress, Budapest, Hungary, 2007.

(vi) Selected Recent Publications Pan L, Sinden MR, Kennedy AH, Chai H, Watson LE, Graham TL, Kinghorn AD. 2008. Bioactive constituents of Helianthus tuberosus (Jerusalem artichoke). Phytochemistry Letters, in press. DOI information: 10.1016/j.phytol.2008.10.003 Graham TL, Graham MY and Yu O. 2008. Genomics of Soybean Secondary Product Metabolism. In: Genetics and Genomics of Soybean, Stacey G, ed. Springer Verlag, NY, pp. 241-242. Graham TL, Subramanian S, Graham MY, Yu O. 2007. RNAi silencing of genes for elicitation or biosynthesis of 5-deoxyisoflavonoids suppresses race specific resistance and hypersensitive cell death in Phytophthora sojae infected tissues. Plant Physiol. 144: 728-740. Subramanian S, Graham MY, Yu O, Graham TL. 2005. RNA interference of soybean isoflavone synthase genes leads to silencing in tissues distal to the transformation site and to enhanced susceptibility to Phytophthora sojae. Plant Physiol. 137:1-9. M. Y. Graham, J. Weidner, K. Wheeler, M. L. Pelow and T. L. Graham. 2003. Pathogenesis-Related Protein Gene Activation in Soybean by Wounding and the Phytophthora sojae Cell Wall Glucan Elicitor, Physiological and Molecular Plant Pathology 63:141-149. T.L. Graham, P.E. Bonello. 2003. Pathogen resistance in plants, McGraw-Hill Yearbook of Science and Technology. Landini, S., Graham, M. Y. and Graham, T. L. 2002. Lactofen induces isoflavone accumulation and glyceollin elicitation competency in soybean. Phytochemistry. Special Issue on Metabolomics. 62:865-874. Park, D.S., Graham, M. Y., Landini, S. and Graham,T.L. 2002. Induced distal defense potentiation against Phytophthora sojae in soybean. Physiological and Molecular Plant Pathology, 60:293-310. Park, D.S., Graham, M. Y. and Graham, T. L. 2001. Identification of soybean elicitation competency factor, CF-1, as the soybean Kunitz trypsin inhibitor. Physiological and Molecular Plant Pathology 59:265-273. Abbasi, P. A., M. Y. Graham and T. L. Graham. 2001. Effects of soybean genotype on the glyceollin elicitation competency of cotyledon tissues to Phytophthora sojae glucan elicitors, Physiological and Molecular Plant Pathology 59:95-105. Abbasi, P. A. and T. L. Graham. 2001. Age-related regulation of induced isoflavone responses in soybean lines differing in inherent elicitation competency. Physiological and Molecular Plant Pathology 59:143-152. Hsieh, M. C. and T. L. Graham. 2001. Partial purification and characterization of a soybean _- glucosidase with high specific activity for isoflavone conjugates. Phytochemistry 58:995-1005. In Preparation: Graham TL, Yu O, Subramanian S, Huge R, Sinden MR, Graham MY. 2008. Effects of RNAi silencing of PR-1a, PR-2 and a unique metallothionein-like gene on partial and race- specific resistance to Phytophthora sojae.

(vii). Synergistic Activities: Ohio State University representative (1996-present; Chair, 2000-2002) to the Ohio Plant Biotechnology Consortium. Panel member for this entire period for their competitive grants program. Associate Editor, Phytopathology (1997-1999). Editorial Board, Plant Physiology (1988-1992) and Journal of Medicinal Food (1998-2002).

(viii) High School, Undergraduate, Graduate and Postgraduate Scholars Sponsored: Thesis advisor for 7 Ph.D. and 1 M.S. student. Student Advisory Committees of over 57 Ph.D. and 12 M.S. students. Hosted over 10 undergraduate researchers and 8 high school students. Many of my students have won awards, a notable one being an undergraduate (Kara Riggs) who was the first freshmen ever to win a Denman Undergraduate Research Award. CURRICULUM VITAE Erich Grotewold Present Address: Plant Biotechnology Center 206 Rightmire Hall, 1060 Carmack Road The Ohio State University, Columbus, OH 43210 Phone: (614) 292-2483; FAX: (614) 292-5379; E-mail: [email protected] Professional Preparation 1988 Ph.D., Chemistry, Instituto de Ingenieria Genetica y Biologia Molecular, and University of Buenos Aires, Buenos Aires, Argentina. 1985 B.Sc., Chemistry, Faculty of Science, University of Buenos Aires, Argentina Academic Appointments 8/08 - present Associate Director, Arabidopsis Biological Resource Center (ABRC), The Ohio State University, Columbus, OH. 9/06 – present Professor, Department of Plant Cellular and Molecular Biology and Department of Horticulture and Crop Sciences, The Ohio State University, Columbus, OH. 9/07 - 6/08 Visiting Professor, Mathematical Bioscience Institute, The Ohio State University, Columbus, OH. 2001 – 2006 Associate Professor, Department of Plant Cellular & Molecular Biology Associate Professor, Department of Horticulture and Crop Sciences, The Ohio State University, Columbus, Ohio 1997 - 2001 Assistant Professor, Department of Plant Biology Assistant Professor, Department of Horticulture and Crop Sciences, The Ohio State University, Columbus, Ohio 1995 - 1997 Assistant Investigator Cold Spring Harbor Laboratory, Cold Spring Harbor, New York. 1993 - 1995 Staff Associate Cold Spring Harbor Laboratory, Cold Spring Harbor, New York. 1989 - 1993 Post-Doctoral Research Fellow Cold Spring Harbor Laboratory, Cold Spring Harbor, New York. Consulting Positions 1/04 – 12/05 Consultant CERES, Inc. Thousand Oaks, CA. Honors and Awards 2006 Dean’s Award for Excellence in Undergraduate Research Mentoring 1995 Demerec-Kaufmann-Hollaender Fellow in Developmental Genetics 1989 - 1990 Long Island Biological Association Fellow 1986 - 1989 Fellow of the National Research Council of Argentina 1985 - 1986 University of Buenos Aires Fellow

Recent Peer Reviewed Publications: 1. Yilmaz, A., Nishiyama, M.Y., Garcia-Fuentes, B., Souza, G.M., Janies, D., Gray, J. and Grotewold, E. (2009) GRASSIUS: A platform for comparative regulatory genomics across the grasses. Plant Physiol. In Press. 2. Xie, Z., and Grotewold, E. (2008) Serial ChIP as a tool to investigate the co-localization or exclusion of proteins on plant genes. Plant Methods 4: 25. 3. Zhao, M., Morohashi, K., Hatlestad, G., Grotewold, E. and Lloyd, A. (2008) The TTG1-bHLH-MYB complex controls trichome cell fate and patterning through direct targeting of regulatory loci. Development. 135: 1991-1999. 4. Grotewold, E. (2008) Transcription factors for predictive plant metabolic engineering: Are we there yet? Curr. Opin. Biotech. 19: 138-144. 5. Hernandez, J.M., Feller, A., Morohashi, K., Frame, K. and Grotewold, E. (2007) The bHLH domain of maize R links transcriptional regulation and histone modifications by recruitment of an EMSY- related factor. Proc. Natl. Acad. Sci. USA. 104: 17222-17227. 6. Morohashi, K., Zhao, M., Yang, M., Read, B., Lloyd, A., Lamb, R., and Grotewold, E. (2007) Participation of the Arabidopsis bHLH factor GL3 in trichome initiation regulatory events. Plant Physiol. 145: 736-746. 7. Poustka, F., Irani, N.G., Feller, A., Lu, Y., Pourcel, L., Frame, K., Grotewold, E. (2007) A trafficking pathway for anthocyanins overlaps with the endoplasmic reticulum-to-vacuole protein sorting route in Arabidopsis and contributes to the formation of vacuolar inclusions. Plant Physiol. 145: 1323- 1335. 8. Serpa, V., Vernal, J., Lamattina, L., Grotewold, E., Cassia, R., Terenzi, H. (2007) Inhibition of AtMYB2 DNA-binding by nitric oxide involves cysteine S-nitrosylation. Biochem. Biophys Res. Comm. 361: 1048-1053. 9. Feller, A., Hernandez, J.M., and Grotewold, E. (2006) An ACT domain participates in the dimerization of several plant bHLH transcription factors. J. Biol. Chem. 281: 28964 – 28974. 10. Vargo, M.A., Voss, O.H., Poustka, F., Cardounel, A.J., Grotewold, E., and Doseff, A.I. (2006) Apigenin-induced-apoptosis is mediated by the activation of PKCδ and caspases in leukemia cells. Biochem. Pharmacol. 72: 681-692. 11. Palaniswamy, K., James, S., Sun, H., Lamb, R., Davuluri, R.V. and Grotewold, E. (2006) AGRIS and AtRegNet: A platform to link cis-regulatory elements and transcription factors into regulatory networks. Plant Phyisiol. 140: 818-829. 12. Grotewold, E. (2006) The genetics and biochemistry of flower pigments. Ann. Rev. Plant Biol. 57: 761-780. 13. Lu, Y., Irani, N.G., and Grotewold, E. (2005) Covalent attachment of the plant natural product naringenin to small glass and ceramic beads. BMC Chemical Biology 5: 3. 14. Molina, C., and Grotewold, E. (2005) Genome-wide analysis of Arabidopsis core promoters. BMC Genomics 6: 25. 15. Barg, R., Sobolev, I., Eilon, T., Gur, A., Chmelnitsky, I., Shabtai, S., Grotewold, E. and Salts, Y. (2005) The tomato early fruit specific gene Lefsm1 defines a novel class of plant-specific SANT/MYB domain proteins. Planta 221: 197-211. Books Edited Grotewold, E. (Editor) The Science of Flavonoids. (2006) Springer, New York, NY. Grotewold, E. (Editor) Plant Functional Genomics: Methods & Protocols. (2003) Humana Press, Totowa, NJ. Patents and Inventions Transgenic plants with altered levels of phenolic compounds. Pat. No. 7,154,023. Transgenic turfgrasses which signal exposure to chemicals and stress conditions. Pat. No. 6,709,867 Inhibition of monocyte survival, differentiation, or proliferation. Application No. PCT/US06/020905

Synergistic Activities: Director, OSU Summer Functional Genomics Training Workshop (2005) Founder, Vice-Chair (2005) and Chair (2007), Gordon Research Conference in Plant Metabolic Engineering Founder and Vice-Chair (2007) Gordon-Kenan Graduate Research Seminars in Plant Metabolic Engineering Member in Interdisciplinary Training Programs: Molecular, Cellular and Developmental Biology (MCDB), Biophysics and Ohio State Biochemistry Program (OSBP) Member, Advisory Committee for the NSF-funded OSU Mathematical Biosciences Institute HAMEL, Patrice

BIOGRAPHICAL SKETCH

NAME POSITION TITLE

Patrice Hamel Assistant Professor

EDUCATION/TRAINING

INSTITUTION AND LOCATION DEGREE YEAR(s) FIELD OF STUDY (if applicable) University of Paris XI, Orsay, France M.S. (eq.) 1993 Cellular and Molecular Genetics University of Paris XI, Orsay, France Ph.D. 1999 Cellular and Molecular Genetics University of California, Los Angeles post-doctoral 1999-2005 Organellar Biochemistry

A. PROFESSIONAL POSITIONS AND HONORS

Previous positions From November 1999-June 2005: Post-graduate Researcher Department of Chemistry & Biochemistry, University of California, Los Angeles

July 2005-October 2005: Assistant Project Scientist, Department of Chemistry & Biochemistry, University of California, Los Angeles

Present positions From November 2005: Assistant Professor, Department of Plant and Cellular Molecular Biology and Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus.

Other professional activities -Reviewer for EMBO Journal, Biochimica and Biophysica Acta, Molecular and General Genetics, Molecular Microbiology, FEBS letters, Proceedings of the National Academy of Sciences USA, Plant Journal, Journal of Biological Chemistry, Eukaryotic Cell, Biochemistry and Journal of Bacteriology. -Ad hoc reviewer for USDA, NSF

Memberships: Associate Investigator, the Dorothy M. Davis Heart and Lung Research Institute, the Ohio State University Member of the Institute of Mitochondrial Biology, The Ohio State University Genetics Society of America

Honors and Awards American Heart Association Post-doctoral Fellowship, 2001-2003 Paul Boyer Award for Excellence in Post-doctoral Research, 2003 American Heart Association Post-doctoral Fellowship, 2003 (declined, duplicate funding) Muscular Dystrophy Association Research Development Award 2003-2006

B. RESEARCH PUBLICATIONS (in chronological order)

(1) Bonnefoy, N., Chalvet, F., Hamel P., Slonimski, P.P. & Dujardin G., (1994) OXA1, a Saccharomyces cerevisiae nuclear gene whose sequence is conserved from prokaryotes to eukaryotes controls cytochrome oxidase biogenesis. J. Mol. Biol., 239, 201-212. - (2) Hamel, P., Sakamoto, W., Wintz, H. & Dujardin, G., (1997) Functional complementation of an oxa1 yeast mutation identifies an Arabidopsis thaliana cDNA involved in the assembly of respiratory complexes. Plant J., 12, 1319-1327. 1 HAMEL, Patrice

(3) Hamel, P., Lemaire, C., Bonnefoy, N., Brivet-Chevillotte, P. & Dujardin G., (1998) Mutations in the membrane anchor of yeast cytochrome c1 compensate for the absence of Oxa1p and generate carbonate- extractable forms of cytochrome c1. Genetics, 150, 601-611.

(4) Cardazzo, B., Hamel, P., Wintz, H., Sakamoto, W. & Dujardin, G., (1998). Isolation of an Arabidopsis thaliana cDNA by complementation of a yeast abc1 deletion mutant deficient in complex III respiratory activity. Gene 221, 117-125.

(5) Arai, M., Hamel, P., Kanaya, E., Inaka, K., Miki, K., Kikuchi, M. & Kuwajima, K. (2000) Effect of an alternative disulfide bond on the structure, stability and folding of human lysozyme. Biochemistry 39, 3472- 3479.

(6) Hamel, P., Olive, J., Pierre, Y., Wollman, F.-A. & de Vitry, C. (2000) A new subunit of cytochrome b6 f complex undergoes reversible phosphorylation upon state transition. J. Biol. Chem. 275, 17072-17079.

(7) Lemaire, C., Hamel, P., Velours, J. and Dujardin, G. (2000) Absence of the mitochondrial AAA protease Yme1p restores ATPase F0 subunit accumulation in an oxa1 deletion mutant of Saccharomyces cerevisiae. J. Biol. Chem. 275, 23471-23475.

(8) Nakamoto, S., Hamel, P. & Merchant, S., (2000) Assembly of chloroplast cytochromes b and c. Biochimie 82, 603-614.

(9) Saint-Georges, Y., Hamel, P., Lemaire, C., and Dujardin, G. (2001) Role of positively-charged transmembrane segments in the insertion and assembly of mitochondrial inner-membrane proteins. PNAS 98, 13814-13819.

(10) Hamel, P., Dreyfuss, B., Xie, Z., Gabilly, S. and Merchant S. (2003) Essential histidine and tryptophan residues in CcsA, a system II polytopic cytochrome c biogenesis protein. J. Biol. Chem. 278, 2593-2603.

(11) Dreyfuss, B., Hamel, P., Nakamoto, S. and Merchant S. (2003) Functional analysis of a divergent system II protein, Ccs1, involved in c-type cytochrome biogenesis. J. Biol. Chem. 278, 2604-2613.

(12) Bernard, D., Gabilly, S., Dujardin, G., Merchant, S. and Hamel, P. (2003) Overlapping specificities of the cytochrome c and c1 heme lyases. J. Biol. Chem. 50, 49732-49742.

(13) Hamel, P., Saint-Georges, Y., Altamura N., de Pinto, B. and Dujardin, G. (2004) Redundancy in the function of mitochondrial phosphate transport in Saccharomyces cerevisiae and Arabidopsis thaliana. Mol. Microbiol. 51, 307-317.

(14) Page, M*., Hamel, P*., Gabilly, S., Zegzouti, H, Perea, J., Alonso, J., Ecker, J., Theg, S., Christensen, S., and Merchant, S. (2004) A homolog of the prokaryotic thiol-disulfide transporter CcdA is required for the assembly of the cytochrome b6f complex in Arabidopsis chloroplasts. J. Biol. Chem. 279, 32474-32482. (* equal contribution).

(15) Bernard, D.B., Quevillon-Cheruel, S., Merchant S., Guiard, B. and P. Hamel (2005) Cyc2p, a membrane bound flavoprotein involved in the maturation of mitochondrial c-type cytochromes. J. Biol. Chem. 280, 39852- 39859.

(16) Hanikenne M., Merchant, S. and Hamel, P. (in press) Transition metal nutrition: a balance between deficiency and toxicity. Chlamydomonas in the Plant Science, Vol.2 of the Chlamydomonas Sourcebook (Ed. D. Stern, Publisher: Elsevier Inc.).

(17) Remacle C., Barbieri, R., Cardol P., and Hamel, P. Eukaryotic complex I: functional diversity and experimental systems to unravel the assembly process Molecular Genetics and Genomics 280, 93-110. 3 HAMEL, Patrice

(18) Hamel P., Corvest V., Giege P and Bonnard G.. Biochemical requirements for the maturation of mitochondrial c-type cytochromes Biochimica Biophysica Acta-Molecular Cell Research (in press, DOI:10.1016/j.bbamcr.2008.06.017).

(19) Claire, R., Cline, S., Boutafalla, L., Gabilly, Larosa, V., Barbieri, R. M. and Hamel, P The ARG9 gene encodes the plastid resident N-acetyl ornitine aminotransferase in the green alga Chlamydomonas reinhardtii Eukaryotic Cell (under revision).

C. RESEARCH SUPPORT

On-going-research support:

Unraveling the mitochondrial redox pathway in cytochrome c maturation Principal investigator: Patrice Hamel Agency: Muscular Dystrophy Association Type: Neuromuscular disease research grant MDA4247 (Years 07-09) Period: 1 Jan, 2007 - 31 Dec, 2009

This project focuses on the genetic and biochemical dissection of the intermembrane space thioredox pathway operating in the maturation of c-type cytochromes in yeast mitochondria.

Molecular genetic dissection of mitochondrial complex I assembly Principal investigator: Patrice Hamel Agency: United Mitochondrial Disease Foundation Type: Research grant Period: 17 Jul, 2007 - 16 June, 2009

This project aims to discover novel assembly factors for mitochondrial complex I using insertional mutagenesis of the nucleus in the green alga Chlamydomonas reinhardtii.

Exploration of cellular functions of organellar coiled-coil proteins in plant model systems. Do plastid and mitochondria have a cytoskeleton-like structure? Principal investigator: Iris Meier Co-principal investigator: Patrice Hamel Agency: Ohio Plant Biotechnology Consortium Type: Research grant Period: 1 Jul, 2008 – 30 June, 2010

This project aims to investigate in Arabidopis and Chlamydomonas the function of a class of novel proteins called coiled-coil proteins with mitochondrial or plastid localization.

Conservation of molecular mechanisms controlling the biogenesis of respiratory and photosynthetic complexes Principal investigator: Geneviève Dujardin (CNRS) Co-principal investigator: Patrice Hamel Agency: Centre National de la Recherche Scientifique (CNRS, France) Type: Travel grant Period: 1 Jan, 2008 – 31 Dec, 2010

This grant aims to support international collaboration at major american institutions for projects that are already on-going in the american host laboratory. The funds can only be used to support the visit of members from a french laboratory to the PI’s group. The co-PI has no financial responsibility over this grant.

Assembly of mitochondrial c-type cytochromes: Emergence of novel redox factors Principal investigator: Vincent Corvest Co-principal investigator: Patrice Hamel Agency: American Heart Association Type: Post-doctoral fellowship Period: 1 Jul, 2008 – 30 June, 2010 3 HAMEL, Patrice The goal of this project is to decipher the biochemical activity of Cyc2p, a novel flavoprotein involved in the assembly of c-type cytochromes in yeast mitochondria.

Regulation of H2 and CO2 Metabolism: Factors Involved in Partitioning of Photosynthetic Reductant in Green Algae Principal investigator: Maria Ghirardi (NREL) Co-principal investigator: Patrice Hamel Agency: Department of Energy Type: Research grant Period: 1 Oct, 2008 – 30 Sept, 2011

The goal of this project is to generate an insertional mutant library in Chlamydomonas and screen for strains that are attenuated for hydrogen production. The long term goal is to uncover the genes controlling this process in order to engineer algal strains that overproduce hydrogen.

Completed research support:

Functional dissection of a cytochrome c assembly machinery in mitochondria Principal investigator: Patrice Hamel Agency: Muscular Dystrophy Association Type: Neuromuscular disease research development grant MDA3618 (Years 01-03) Period: 1 Jul, 2003 - 30 Sept, 2006 (no cost extension)

This project focuses on the functional identification of novel components involved in a c-type cytochrome assembly complex in yeast mitochondria

Unravelling the mechanisms of cytochrome c biogenesis Principal investigator: Patrice Hamel Agency: American Heart Association Western Affiliates Type: Post-doctoral Fellowship 0120100Y (Years 01-02) Period: 1 Jul,2001 - 30 Jun, 2003

The goals of this project were to define the biochemistry of well-characterized c-type cytochrome assembly components in plastids of green alga and mitochondria of yeast and discover novel biogenesis factors.

3 CURRICULUM VITAE

Jyan-Chyun Jang

Professional Preparation Natl. Taiwan University Forest Botany 1980 B.Sc. Natl. Taiwan University Plant Pathology 1984 M.Sc. Clemson University Forest Pathology 1989 Ph.D. Inst. of Biological Chemistry Washington State Univ. Molecular biology 1990-1991 Postdoctoral Researcher Department of Genetics Harvard Medical School Genetics 1991-1997 Postdoctoral fellow Dept. of Mol. Biol., Mass. General Hospital Molecular Biology 1991-1997 Postdoctoral fellow

Appointments 2003-present Associate Professor Dept. of Horticulture and Crop Science Dept of Plant Cellular and Molecular Biology, The Ohio State University 1997-2002 Assistant Professor Dept. of Horticulture and Crop science Dept of Plant Biology, The Ohio State University 1997-present Member Plant Molecular Biology and Biotechnology Program Program in Molecular, Cellular and Developmental Biology, The Ohio State University

Recent Publications 1. He, J. X., Fujioka, S., Li, T.-C., Kang, S. G., Seto, H., Takatsuto, S., Yoshida, S., and Jang, J.-C. (2003) Sterols regulate development and gene expression in Arabidopsis thaliana. Plant Physiol. 131:1258-1269. 2. Price, J., Li, T. C., Kang, S. G., Na, J. K., and Jang, J.-C. (2003). Mechanisms of glucose signaling during seed germination of Arabidopsis thaliana. Plant Physiol. 132 (3): 1424-1438. 3. Price, J., Laxmi, A., St. Martin, S. K., and Jang, J.-C. (2004) Global transcription profiling reveals multiple sugar signal transduction mechanisms in Arabidopsis. Plant Cell 16:2128-2150. 4. Shao, M., Zheng, H., Hu, Y., Liu D., Jang, J-C., Ma, H., and Huang, H. (2004) The GAOLAOZHUANGREN1 gene encodes a putative glycosyltransferase that is required for normal development and affects sink-source transition in Arabidopsis. Plant Cell Physiol. 45:1453-1460. 5. Zheng Z-L, Yang Z, Jang J-C and Metzger JD (2006) Phytochromes A1 and B1 have distinct functions in the photoperiodic control of flowering in the obligate long-day plant Nicotiana sylvestris. Plant Cell & Environment 29: 1673-1685

Current Advising: Graduate (Ph.D.): Marcelo Pomeranz, Srimathi Bogamuwa, Jie Qu Graduate (MS): Li Zhang Postdoc: Shin Gene Kang

Past Thesis Advisor and Postdoc Sponsor PhD: Wenyan Xiao (Faculty, St. Louis University), 1997-2001 Shin Gene Kang (Postdoc, OSU), 1999-2004 Jong Kuk Na (Postdoc, U of Illinois), 2001-2005 Tsai-Chi Li (Graduate student, OSU), 2001-2006 Pei-Chi Lin (deceased), 2004-2007 MS: Marie Purtell (Kendal International, Cincinnati, OH), 2001-2003. Postdoc: Jun Xian He (Faculty, Chinese University of Hong Kong), 2000-2001 Ashverya Laxmi (Faculty, University of Delhi, India), 2003-2004 Minghai Shao (Postdoc, Children’s Hospital, Columbus, OH), 2004 Sholpan Devaletova (Postdoc, Children’s Hospital, Columbus, OH), 2004 Janaiah Kota (Postdoc, Children’s Hospital, Columbus, OH), 2005

Current Course Taught 694 Microarray Analysis for Gene Expression Profiling 830 Signal Transduction of Plant Growth Substances 830 Research Methods: Transient Gene Expression

Synergistic Activities Grant Panel: USDA CSREES (2007) Ad Hoc Review: NSF, USDA, BARD, Irish National Science Foundation, CUNY Journal Article Review: Arch Biochem Biophy, BMC Systems Biology, BMC Plant Biology, Functional Plant Biology, Genome Research, J of American Society for Horticultural Science, J. Experimental Botany, J. of Plant Growth Regulation, Molecular Biology Reports, Molecular Plant, New Phytologists, Physiol Plant, Plant Cell, Plant Cell and Environment, Plant Cell Physiology, Plant Cell Rept, Plant Journal, Plant Mol Biol, Plant Physiology, Plant Physiol Biochem, Planta, PLoSONE, PNAS, Seed Science & Technology, Trends Plant Sci.

Reecent Extramural Fundings USDA CSREES (2005-3531915318) Jan. 1, 2005 to Dec. 31, 2007 $387,500 JC Jang (PI), Wolfgang D. Bauer (Co-PI)

NSF Biological Infrastructure (DBI-0500601) May 1, 2005 to April 30, 2008 $67,111 David Somers (PI), Erich Grotewold (Co-PI), JC Jang (Co-PI)

NSF Integrative Organismal Biology (IOB-043751) Feb. 1, 2006 to Jan. 31, 2010 $480,000 JC Jang (PI) BIOGRAPHICAL SKETCH

MICHELLE L. JONES Department of Horticulture and Crop Science, The Ohio State University/ OARDC Wooster, OH, 44691; Tel: 330-263-3885; Fax: 330-263-3887; email: [email protected]

Professional Preparation: Iowa State University, Ames, IA B.S. 1993 Agricultural Biochemistry Purdue University, West Lafayette, IN Ph.D. 1997 Horticulture

Professional Experience: 2008- present D.C. Kiplinger Endowed Chair in Floriculture Dept. of Horticulture and Crop Science The Ohio State University, Wooster, OH 2006- present Associate Professor, Dept. of Horticulture and Crop Science, The Ohio State University, Wooster, OH Area of Specialization: Floriculture Molecular Biology 2001- 2006 Assistant Professor, Dept. of Horticulture and Crop Science, The Ohio State University, Wooster, OH 1998- 2001 Assistant Professor, Dept. of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, CO.

Publications (5 most relevant): Jones M.L. (2008) Ethylene signaling is required for pollination-accelerated corolla senescence in petunias. Plant Science 175: 190-196.

Chapin L., and M.L. Jones (2007) Nutrient remobilization during pollination-induced corolla senescence in petunia. Acta Horticulturae 755: 181-190.

Jones M.L., G.S. Chaffin, J.R. Eason and D. Clark (2005) Ethylene Sensitivity Regulates Proteolytic Activity and Cysteine Protease Gene Expression in Petunia Corollas. Journal of Experimental Botany. 56:2733-2744.

Chiang Y-C., C. Stushnoff, A.E. McSay, M.L. Jones and H. Bohnert (2005) Overexpression of Mannitol-1-Phosphate Dehydrogenase Increases Mannitol Accumulation and Adds Protection Against Chilling Injury in Petunia. Journal of the American Society for Horticultural Science. 130:605-610.

Langston B.L., S. Bai and M.L. Jones (2005) Increases in DNA Fragmentation and Induction of a Senescence-Specific Nuclease are delayed during the Senescence of Ethylene-insensitive (etr1-1) Transgenic Petunias. Journal of Experimental Botany. 56:15-23.

Publications (5 significant): Jones, M.L., Shuangyi B., Willard B., Stead A., and M. Kinter (2007) Proteomic Analysis of Pollination-induced Senescence in Petunia Flowers. Proceedings of the 7th International Ethylene 1 Symposium. A. Ramina, C. Chang, J. Giovannoni, H. Klee, P. Perata, and E. Woltering eds. Springer, The Netherlands, Pgs. 279-284.

Pennycooke J.C., R. Vepachedu, C. Stushnoff and M.L. Jones (2004) Expression of an α- Galactosidase Gene in Petunia is Up-regulated during Low Temperature Deacclimation. Journal of the American Society for Horticultural Science. 129:491-496.

Clark D.G., C. Dervinis, J.E. Barrett, H. Klee and M.L. Jones (2004) Drought-induced Leaf

Senescence and Horticultural Performance of Transgenic PSAG12-IPT Petunias. Journal of the American Society for Horticultural Science. 129: 93-99.

Pennycooke J.C., M.L. Jones and C. Stushnoff (2003) Down-Regulating α-Galactosidase Enhances Freezing Tolerance in Transgenic Petunia. Plant Physiology. 133: 901-909.

Chang H., M.L. Jones, G.M. Banowetz and D.G. Clark (2003) Overproduction of Cytokinins in

Petunia Flowers Transformed with PSAG12-IPT Delays Corolla Senescence and Decreases Sensitivity to Ethylene. Plant Physiology. 132: 2174-2183.

Synergistic activities: Consulting Editor, Molecular Biology and Biotechnology, The Journal of the American Society for Horticultural Science, 2004 to present. Member Editorial Board, Postharvest Biology and Technology, 1999 to 2004.

Collaborators and Other Affiliations: Collaborators and Co-authors: Bai, Shuangyi (Washington State); Banowetz, Gary (ARS, Corvallis); Barrett, Jim (U Florida): Bohnert, Hans (U of Illinois); Canas Luis (Ohio State); Chaffin, Gunching (Unknown); Clark, David (U Florida); Dervinis, Chris (U Florida); Eason, Joycelyn (Food & Crop Research, NZ); Finer, John (Ohio State); Francis, David (Ohio State); Franz, Jonathan (ARS, Toledo); Johnson, Felicity (Unnknown); Kinter, Michael (Cleveland Clinic, OH); Klee, Harry (U Florida); Langston, Brennick (TX A&M); Lewandowski, Dennis (Ohio State); Ling, Peter (Ohio State); Lopez, Roberto (Purdue), Meulia, Tea (Ohio State); Pasian, Claudio (Ohio State); Pennycooke, Joyce (Seminis, CA); Stead, Anthony (Royal Holloway, UK); Stushnoff, Cecil (Colorado State); Willard, Belinda (Cleveland Clinic, OH), Woodson, William (Purdue)

Advisors: William R. Woodson, Purdue University Current Advisees: Ph.D. Nicole Waterland, Youyoun Moon Past Advisees: Postdoctoral researchers: Gunching Chaffin, Felicity Johnson Ph.D. Shuangyi Bai (Washington State Univ), Hsiang Chang (Chungchou Institute of Technology, Taiwan), Joyce Pennycooke (Seminis)

2 M.S. Brennick Langston (Texas A&M Univ)

3 BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Lamb, Rebecca Sarah Assistant Professor Plant Cellular and Molecular eRA COMMONS USER NAME Biology

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) Wesleyan University Middletown, CT BA 1992 Biology/German Studies Duke University Durham, NC Ph.D. 1998 Zoology and Cell and Molecular Biology

Yale University New Haven, CT NA 1998-2003 Floral development in Arabidopsis thaliana

A. Positions and Honors.

Positions and Employment 1998-2003 Postdoctoral Researcher, Yale University, New Haven, CT 2003- Assistant Professor, Plant Cellular and Molecular Biology, Plant Molecular Biology and Biotechnology Program, and The Molecular, Cellular, and Developmental Biology Program, The Ohio State University

Professional Memberships 1999- Member, American Society of Plant Biologists 2003- Member, American Society for the Advancement of Science 2003- Member, Genetics Society 2004- Member, Society for Developmental Biology 2004- Member, FASEB 2007- Member, Association for Women in Science

Professional Activities 2003- Reviewer, Plant Cell, Plant Science, PNAS, Plant Physiology, Development, Science, Nature 2005- Panelist, National Science Foundation, Epigenetics, Transcription and Chromatin Panel within the Genes and Genome Systems, BIO/MCB

Fellowships and Honors 1988-1992 Hedden Scholarship, Wesleyan University, Middletown, CT 1992 Blankennagel Prize in German, Wesleyan University, Middletown, CT 1992-1995 NIH Training Grant in Cell and Molecular Biology, Duke University, Durham, NC 1998-1999 Brown Postdoctoral Fellowship, Yale University, New Haven, CT

B. Selected peer-reviewed publications (in chronological order). 1. Morohashi, K, Zhao, M, Yang, M, Read, B, Lloyd, A, Lamb R, and Grotewold, E (2007). Participation of the Arabidopsis bHLH factor GL3 in trichome initiation regulatory events. Plant Physiology 145(3): 736-746.

2. Palaniswamy, S., James, S., Sun H, Lamb, R.S., Davuluri, R.V., Grotewold, E (2006). AGRIS and AtRegNet: A platform to link cis-regulatory elements and transcription factors into regulatory networks. Plant Physiology 140(3): 818-829.

3. Lamb, R.S. and Irish, V.F. (2003) Functional divergence within the APETALA3/PISTILLATA floral homeotic gene lineages. Proc. Nat. Acad. Sci. 100: 6558-6563.

4. Lamb, R.S., Hill, T.A., Tan, Q.K., and Irish, V.F. (2002) Regulation of APETALA3 floral homeotic gene expression by meristem identity genes. Development 129: 2079-86.

5. Ward, R.E., Schweizer, L, Lamb, R.S., and Fehon, R.G. (2001) The protein 4.1, radixin, moesin (FERM) domain of Drosophila Coracle, a cytoplasmic component of the septate junction, provides functions essential for embryonic development and imagninal cell proliferation. Genetics 159: 219- 228.

6. Lamb, R.S., Ward, R.E., Schweizer, L. and Fehon, R.G. (1998) Drosophila coracle, a member of the protein 4.1 superfamily, has essential structural functions in the septate junctions and developmental functions in the embryonic and adult epithelial cell. Mol. Biol. Cell 9: 3505-3519.

7. Ward, R.E., Lamb, R.S., and Fehon, R.G. (1998) A conserved functional domain of Drosophila Coracle is required for localization at the septate junction and has membrane organizing activity. J. Cell Biol. 140: 1463-1473.

8. Fehon, R.G., LaJeunesse, D, Lamb, R.S., McCartney, B.M., Schweizer, L., and Ward, R.E. (1997) Functional studies of the protein 4.1 family of junctional proteins in Drosophila. Soc. Gen. Physiol. Ser. 52: 149-159.

C. Research Support

Ongoing Research Support

Ohio Plant Biotechnology Consortium Research Grant 7/1/2007-7/30/2009 Two Arabidopsis WWE-PARP Proteins Involved in Abiotic Stress Response and Development. The major goal of this project is characterize the phenotypic and molecular phenotypes of two Arabidopsis genes that appear to be involved in epigenetic control of transcription in plants. Role: Principal Investigator

Completed Research Support

MCB-0418891 Grotewold (PI) NSF 9/1/2004-8/31/2007 Arabidopsis 2010: Establishing Regulatory Networks in Arabidopsis: Integrating AGRIS with the Identification of Direct Targets for Transcription Factors The goal of this research was to determine the genes directly controlled by a set of plant transcription factors. Role: Co-investigator

BIOGRAPHICAL SKETCH

Brian B. McSpadden Gardener Department of Plant Pathology The Ohio State University/OARDC, Wooster, OH 44691; Tel: 330-2202-3565; FAX: 330-263-3841; email: [email protected]; web: http://www.oardc.ohio-state.edu/mcspaddengardenerlab/

PROFESSIONAL PREPARATION University of Illinois Honors Biology B.Sc. 1992 Michigan State University Botany Ph.D. 1998 Washington State University Microbial diversity/ecology Postdoc. 1998-2000

PROFESSIONAL EXPERIENCE 10/06 to Present Associate Professor, Department of Plant Pathology, Ohio State U. 11/00 to 09/06 Assistant Professor, Department of Plant Pathology, Ohio State U.

PUBLICATIONS most closely related to the project: Benitez, M.-S., Baysal Tustas, F., Rotenberg, D., Kleinhenz, M.D., Miller, S.A., and McSpadden Gardener, B.B. 2007. Multiple statistical approaches of community fingerprint data reveal bacterial populations associated with general disease suppression arising from the application of different organic field management strategies. Soil Biology and Biochemistry 39:2289-2301 Rotenberg, D. Joshi, R. Benitez, M.S., Gutierrez Chapin, L., Camp, A., Zumpetta, C., Osborne, A., Dick, W.A., and McSpadden Gardener, B.B. 2007. Complex effects of farm management on rhizosphere colonization by native populations of phlD-containing Pseudomonas spp. and the relative contribution of those bacteria to crop stand and productivity. Phytopathology 97:756-766. Lewis Ivey, M.L., McSpadden Gardener, B.B., Opina, N., and Miller, S.A. 2007. Diversity and geographic distribution of Ralstonia solanacearum strains isolated from eggplant in the Luzon Region of the Philippines. Phytopathology 97:1467-1475. McSpadden Gardener, B. 2006. Statistical analyses of microbiological and environmental data. Chapter 22, pp. 555-585 in Modern Soil Microbiology, 2nd ed. J.D. van Elsas, J. Jannson eds. CRC Press: Boca Raton, FL. Joshi, R., and McSpadden Gardener, B. 2006. Identification of genes associated with pathogen inhibition in different strains B. subtilis. Phytopathology 96:145-154.

Other significant publications: Borneman, J., Becker, J.O., Bent, E., Lanoil, B., McSpadden Gardener, B., Olatinwo, R., Presley, L., Scupham, A.J., Valinsky, L., and Yin, B. 2007. Identifying microorganisms involved in specific in situ functions: Experimental design considerations for rRNA gene-based population studies and sequence-selective PCR assays. Pages 748-757. in Manual of Environmental Microbiology, 3rd ed. ASM Pres: Washington, D.C. Gutierrez Chapin, L., Want, Y., Lutton, E., and McSpadden Gardener, B. 2006. Distribution and fungicide sensitivity of tomato fruit rot pathogens in Ohio. Plant Disease 90:397-403 McSpadden Gardener, B., Gutierrez, L., Joshi, R., Edema, R., and Lutton, E. 2005. Distribution and biocontrol potential of phlD+ pseudomonads in corn and soybean fields. Phytopathology 95:715-724. Blouin Bankhead, S., Landa, B., Lutton, E., and Weller D., McSpadden Gardener, B. 2004. Minimal changes in rhizobacterial population structure following root colonization by wild type and transgenic biocontrol strains. FEMS Microbiol. Ecol. 49:307-318. McSpadden Gardener B., and Weller D. 2001. Changes in populations of rhizosphere bacteria associated with take-all disease of wheat. Appl. Environ. Microbiol. 67:4414-4425 SYNERGISTIC ACTIVITIES Multi-state research committees related to biological control: NC-125 (2002-2004), W-1147 (2006- Present) American Phytopathological Socieity Editorships: Assoc. Editor Appl. Environ, Microbiol. (2008- Present), Senior Editor APS Press (2007-Present),Assoc. Editor Phytopathology (2003-2006), American Phytopathological Society, Biological Control Committee; member, Chair 2003, web-site developer and manager (2003-Present), Symposium organizer (2003-2006) Poster session organizer, 18th World Congress of Soil Sciences (2005-2006) Undergraduate Internship Coordinator Plant Molecular Biology and Biotechnology Program (2007- Present) Graduate Teaching: PLNT PTH 655: Quantitative Methods in Applied Biology (100%) PLNT PTH 602: Plant-Microbe Interactions (10%) PLNT PTH 843: Ecology of Plant-Associated Microbes (50%) PLNT PTH 830: Biological Control of Plant Pathogens (100%)

COLLABORATORS AND COAUTHORS Beuerlein, James (Ohio State U.), Boehm, Mike (Ohio State U.), Bonello, Pieluigi (Ohio State U.), Borneman, James (U. California), Cardina, John (Ohio State U.), Dorrance, Anne (Ohio State U.), Dick, Warren (Ohio State U.), Dick, Richard (Ohio State U.), Driks, Adam (Loyola U.), Edema, Richard (Makere U.), Edwards, Clive (Ohio State U.), Fravel, Deborah (USDA ARS), Fuerst, Paul (Ohio State U.), Graham, Terry (Ohio State U.), Grewal, Parwidner (Ohio State U.), Grotewald, Erich (Ohio State U.), Hatcher, Patrick (Ohio State U.), Hoitink, H.A.J. (Ohio State U.), Hoy, Casey (Ohio State U.), Kinkel, Linda (U. Minnesota), Kleinhenz, Matthew (Ohio State U.), Landa, Blanca B. (U. Cordoba), Louws, Frank (NCSU), Mavrodi, Dmitri (Washington State U.), Meyer, Susan (USDA-ARS), Michel, Fred (Ohio State U.), Miller, Sally A. (Ohio State U.), Nakatsu, Cindy (Purdue U.), Pan, Jean (Akron U.), Paul, Eldor (Michigan State U.), Raaijmakers, Jos (Wageningen U.), Read, Timothy (U. of Maryland), Robertson, Philip (Michigan State U.), Rupe, John (U. Arkansas), Schroeder, Kurt (Washington State U.), Smart, Christine (Cornell U.), Stinner, Deborah (Ohio State U.), Thomashow, Linda (Washington State U.), Thomison, Peter (Ohio State U.), Tiedje, James (Michigan State U.), van der Knaap, Eshter (Ohio State U.), Wang, Koon-Hui (U. Florida), Wszelaki, Annette (U. of Puerto Rico).

ADVISORS De Bruijn, Frans J. (INRA France) Van Elsas, Jan D. (U. Groningen, Netherlands) Nadler, Kenneth D. (Michigan State University) Weller, David M. (Washington State University)

ADVISEES Current: Ph.D. Benitez, M. Soledad M.S. Raudales, Rosa Past: M.S. Gutierrez-Chapin, Laura (Ohio State U.), Joshi, Raghavendra (U. Minnesota), Postdoctoral Researchers: Rotenberg, Dorith (Kansas State U.), Grewal, Suhkbir (Ohio State U.), Wang, Ying (U. Tennessee) BIOGRAPHICAL SKETCH Provide the following information for the key personnel in the order listed for Form Page 2. Follow the sample format for each person. DO NOT EXCEED FOUR PAGES.

NAME Professor of Plant Cellular and Molecular Biology Meier, Iris

POSITION TITLE

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) INSTITUTION AND LOCATION DEGREE YEAR(s) FIELD OF STUDY (if applicable) Technical University M.S. 1984 Microbiology Darmstadt, Germany University of Duesseldorf, Ph.D. 1987 Molecular Biology Germany

Max Planck Institute for Plant Postdoc. 1987-1990 Plant Molecular Breeding, Cologne, Germany. Biology Postdoc. University of California at Postdoc. 1990-1993 Plant Molecular Berkeley, CA Biology

A. Positions and Honors. Positions and Employment 1993 - 1996 Junior Group Leader, Applied Plant Molecular Biology, University of Hamburg, Germany. 1997 - 1999 Senior Research Scientist, DuPont Central Research and Development, Wilmington, DE. 1999 – 2003 Assistant Professor, Dept. of Plant Biology, Ohio State University. 2003 – 2008 Associate Professor, Dept. of Plant Cellular and Molecular Biology, Ohio State University. 2008- Professor, Dept. of Plant Cellular and Molecular Biology, Ohio State University.

Other Experience and Professional Memberships 1999- Ad hoc grant reviewer for: NSF, USDA, DOE, BBSRC (UK), The Leverhulme Trust (UK), BARD (The United States - Israel Binational Agricultural Research & Development Fund). 1999- Reviewer: The Plant Cell, The Plant Journal, Plant Physiology, Journal of Cell Biology, Transgenic Research, Planta, Plant Molecular Biology, Trends in Plant Sciences, Nucleic Acids Research, Traffic, Biotechniques, Plant Biology. 2001- Panel member: National Science Foundation, Cellular Organization Panel; United States Department of Agriculture CSREES, Developmental Processes of Crop Plants Panel; BARD (The United States – Israel Binational Agricultural Research and Development Fund): Model Systems and Functional Biology Panel. 2002 - 2003 International Advisory Committee: Society for Experimental Biology (SEB) meeting "The Nuclear Envelope: Signaling, Communication and Gene Regulation", 2003, Durham, UK. 2004 - Advisory Board: NSF Plant Genome project, “Global analysis of functional units in plant chromosomes: DNA replication, domain structure, and transcription.” 2006 - Associate Editor for Plant Molecular Biology 2006 – 2008 Co-Organizer, Nuclear envelope and Golgi, Cell Biology Satellite, SEB Marseille 2008 Honors 1984 - 1986 Ph.D. fellowship by the “Fond der Chemischen Industrie”, Germany 1988 - 1990 Post-doctoral fellowship by the Max-Planck-Society, Germany 1992 - 1993 Research Fellow of the German Science Foundation (DFG) 2006 Dean’s Award for Excellence in Research and Graduate Education, College of Biological Sciences, OSU

B. Selected peer-reviewed publications (selected from 52 research publications) 1. Meier, I., Wray, L.V., Jr., and Hillen, W. (1988). Differential regulation of the Tn10 encoded tetracycline resistance genes tetA and tetR by the tandem tet operators O1 and O2. EMBO J. 7, 567-572. 2. van de Löcht, U., Meier, I., Hahlbrock, K., and Somssich, I. E. (1990). A 125 bp promoter fragment is sufficient for strong elicitor-mediated gene activation in parsley. EMBO J. 9, 2945-2950. 3. Meier, I., Hahlbrock, K., and Somssich, I. E. (1991). Elicitor-inducible and constitutive in vivo DNA footprints indicate novel cis-acting elements in the promoter of a parsley gene encoding pathogenesis- related protein 1. Plant Cell 3, 309-315. 4. Meier, I., Callan, K. L., Fleming, A. J., and Gruissem, W. (1995). Organ-specific differential regulation of a promoter subfamily for the ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit genes in tomato. Plant Physiol. 107, 1105-1118. 5. Meier, I., Phelan, T., Gruissem, W., Spiker, S., and Schneider, D. (1996). MFP1, a novel plant filament-like protein with affinity for matrix attachment region DNA. Plant Cell 8, 2105-2115. 6. Rose, A., Meier, I., and Wienand, U. (1999). The tomato I-box binding factor LeMYBI is a member of a novel class of Myb-like proteins. Plant J. 20, 641-652. 7. Gindullis, F., and Meier, I. (1999). Matrix Attachment Region Binding Protein MFP1 Is Localized in Discrete Domains at the Nuclear Envelope. Plant Cell 11, 1117-1128. 8. Gindullis, F., Peffer, N.A., and Meier I. (1999). MAF1, a novel plant protein interacting with MAR- binding protein MFP1 is located at the nuclear envelope. Plant Cell 11, 1755-1767. 9. Rose, A. and Meier, I. (2001) A domain unique to plant RanGAP is responsible for its targeting to the plant nuclear rim. Proc. Natl. Acad. Sci. U.S.A. 98, 15377-15382. 10. Calikowski, T.T., Meulia, T., and Meier, I. (2003) A Proteomic Study of the Arabidopsis Nuclear Matrix. J. Cell. Biochem. 90, 361-378. 11. Rose, A., Manikantan, S., Schraegle, S., Maloy, M., Stahlberg, E. and Meier, I. (2004) Genome-wide Identification of Arabidopsis Coiled-coil Proteins and Establishment of the ARABI-COIL Database. Plant Physiol. 134: 927-939. 12. Patel S, Rose A, Meulia T, Dixit R, Cyr RJ, Meier, I. (2004). Arabidopsis WPP-Domain Proteins Are Developmentally Associated with the Nuclear Envelope and Promote Cell Division. Plant Cell 16:3260-3273. 13. Jeong, SY, Rose S, Joseph, J, Dasso, M, and Meier, I. (2005). Plant-specific mitotic targeting of RanGAP requires a functional WPP domain. Plant J 42:270-82. 14. Rose A, Schraegle SJ, Stahlberg EA and Meier, I (2005). Coiled-coil protein composition of 22 proteomes - differences and common themes in subcellular infrastructure and traffic control. BMC Evol. Biol 5:66. 15. Xu X, Rose A, Muthuswamy S, Jeong S-Y, Venkatakrishnan S, Zhao Q, and Meier I. (2007). NUCLEAR PORE ANCHOR, the Arabidopsis Homolog of Tpr/Mlp1/Mlp2/Megator, Is Involved in mRNA Export and SUMO Homeostasis and Affects Diverse Aspects of Plant Development. Plant Cell 19: 1537-1548. 16. Xu X, Meulia T and Meier I. (2007). Anchorage of Plant RanGAP to the Nuclear Envelope Involves Novel Nuclear-Pore-Associated Proteins. Curr Biol. 17: 1157-1163. 17. Zhao, Q, Brkljacic, J, and Meier, I. (2008) Two distinct, interacting classes of nuclear envelope- associated coiled-coil proteins are required for the tissue-specific nuclear envelope targeting of Arabidopsis RanGAP. Plant Cell 20, 1639-1651. 18. Xu, X, Zhao, Q, Rodrigo-Peiris, T, Brkljacic, J, He, C, Mueller, S and Meier I. (2008) RanGAP1 is a continuous marker of the Arabidopsis cell division plane. Proc. Natl. Acad. Sci. USA, in press. 19. Xu XM and Meier I. (2008). The Nuclear Pore Comes to the Fore. Trends in Plant Sci. 13, 1-50. 20. Brkljacic J and Meier I (2008). The nuclear pore and plant development. Curr. Opin. Plant Biol., in press.

C. Research Support

Ongoing Research Support 04/07 – 03/10 National Science Foundation -Molecular and Cellular Biosciences -0641271 “Arabidopsis as a new experimental platform to investigate the function of the nuclear pore protein Tpr in SUMOylation and mRNA export.” Principle investigator. The objective of this grant is the functional investigation of the plant nucleoporin NUA/Tpr and how it relates to the connection between SUMOylation and nuclear RNA export.

Completed Research Support 04/08-03/09 National Science Foundation Molecular and Cellular Biosciences - 0805009 Group travel proposal: Nuclear Envelope and Golgi, Marseille 2008. Principle investigator. The purpose was to bring together US and European researchers to discuss connections between the nuclear envelope and Golgi in plants. 05/04 - 04/08 National Science Foundation -Molecular and Cellular Biosciences -0343167 "Investigating Structure, Function, and Evolution of a Plant-Speciﬁc Nuclear-Targeting Domain" Principle investigator. The overall goal of this grant was to understand the molecular mechanism of subcellular anchoring of plant RanGAP and how it relates to the functions of the Ran cycle in plant interphase and mitosis. 09/02 – 08/06 National Science Foundation - Molecular and Cellular Biosciences- 0209339: “Arabidopsis 2010: Investigating coiled-coil proteins in the Arabidopsis ORFeome” Principle investigator. The purpose of this project was to computationally identify all long coiled-coil proteins in Arabidopsis and functionally investigate a subgroup associated with the nucleus. 09/01 - 08/05 USDA Plant Growth and Development: “Anchoring of Ran-mediated signal transduction in plants”. Principle Investigator. The purpose of this research was to identify the unique aspects of RanGAP subcellular localization in plants. 09/01 - 08/05 National Science Foundation - Molecular and Cellular Biosciences 0079507: “Investigating the role of novel nuclear envelope-associated plant proteins in nuclear dynamics” The main goal of this work was to characterize a plant-unique mechanism of nuclear envelope targeting and identify proteins involved in the mechanism. Tea Meulia Molecular and Cellular Imaging Center The Ohio State University-Ohio Agricultural Research and Development Center Wooster, OH 44691 Tel: 330-263-3836 FAX: 330-202-3563 email: [email protected]

Professional Preparation University of Lausanne, Switzerland Biology/Biochemistry BS/MS 1985 Swiss Institute for Experimental Cancer Research, Switzerland Genetics Ph.D. 1990 Fred Hutchinson Cancer Research Center, Seattle, WA. Genetics PostDoc 1990-94 OARDC Ohio State University Plant Pathology PostDoc 1995-98

Appointments 1999-date Research Scientist and Director of the Molecular and Cellular Imaging Center (http://www.oardc.ohio-state.edu/mcic), a shared technology facility at The Ohio State University, OARDC, Wooster, OH. 1998-1999 Research Scientist split position in the department of Horticulture and Crop Sciences, with Dr. David Francis, and the Electron Microscopy Laboratory, The Ohio State University, OARDC, Wooster, OH. 1995-1998 Research Associate in Plant Pathology with Dr. Donald Gordon, The Ohio State University, OARDC, Plant Pathology Wooster, OH. 1990-1994 Post-doctoral associate with Dr. Mark Groudine at the Fred Hutchinson Cancer Research Center, Seattle, WA 1985-1990 High School Teacher at the Liceo Pareto, Leusanne, Switzerland. 1983-1986 Teaching Assistant for genetics courses and laboratory with Prof. Walter Wahli, University of Lausanne, College of Biology, Lausanne, Switzerland.

Publications in the past five years Rajakaruna, P., Khandekar, S., Meulia, T., and Leisner, S.M. 2007. Identification and host relations of a novel comovirus from Ohio. Plant Disease. 91:1212-1220. Xu, X., Meulia, T. and Meier, I. Anchorage of plant RanGAP to the nuclear envelop involves novel nuclear-pore-associated proteins. 2007. Current Biology. 17:1157-63. Fraga, D., Meulia, T. and Fenster, S. Real-time PCR. 2007. Chapter 32 in ‘Current Protocols in Essential Laboratory Techniques.’ John Wiley & Sons Publishing. In Press. Welty, N., Radovic, C., Meulia, T., and Van Der Knaap, E. 2007. A developmental analysis of flower and fruit formation in LA1589, a wild relative of tomato. Canadian Journal of Botany.85:111-118. Kanneganti, T., Bai, X., Tsai, C., Win, J., Meulia, T., Goodin, M., Kamoun, S., and Hogenhout, S. A. 2007. A functional genetic assay for nuclear trafficking in plants. The Plant Journal, 50:149-158. Cheetham, S., Souza, M., McGregor, R., Meulia, T., Wang, Q. and Saif, L.J. Binding patterns of Human Norovirus-like particles to buccal and intestinal tissues of gnotobiotic pigs in relation to A/H histo- blood group antigen expression. 2007. J. Virol. 81: 3535-3544. Cheetham, S., Souza, M., Meulia, T., Grimes, S., Han, M. G., and Saif, L. J. 2006. Pathogenesis of a genogroup II human norovirus in gnotobiotic pigs. J. Virol. 80:10372-81. Radovic, C.; Van Der Knaap, E.; Meulia, T. 2004. Flower development of Lycopersicon pimpinellifolium LA1589, a close relative of cultivated tomato. Molecular Biology of the Cell 15(Suppl.S) p.217A. Patel.S., Rose,A. Meulia, T., Dixit, R., Cyr, R.J. and Meier,I. 2004. Arabidopsis WPP-domain proteins are developmentally associated with the nuclear envelope and promote cell division. The Plant Cell. 16, 3260-3273. Ammar,E.D., Meulia, T. Özbek E. and Hogenhout,S.A. 2004. Assembly and accumulation sites of maize mosaic virus (Rhabdoviridae) in plant host and insect vector using transmission electron and confocal laser microscopy. Book chapter. Current Issues on Multidisciplinary Microscopy Research and Education. A. Mendez-Vilas and L. Labajos-Broncano [eds.]. Formatex Microscopy Book Series, no.2, Badajoz, Spain. Pp. 56-64. Ammar, E.D., Fulton, D., Bai, X., Meulia, T. and Hogenhout, S.A. (2004). An attachment tip and pili-like structures in insect- and plant-pathogenic spiroplasmas of the class Mollicute. Arch. Microbiol. 181:97-105

Awarded proposals in the past five years Hogenhout, S.A. Coplin, D.L., and Meulia, T. The nature of the association of Pantoea stewartii, the causative agent of Stewart’s bacterial wilt of corn, with its flea beetle vector, Chaetocnema pulicaria. 2006. OARDC Interdisciplinaty Team Research Competition. ($11,000 to the MCIC). Myers,S., Meulia, T., et al. Equipment and resources for increasing genotyping capabilities at the (Molecular and Cellular Imaging Center) MCIC for the development of value-added soybean and other crops for Ohio. Ohio BioProducts Innovation Center. (Wright Centers of Innovation, Third Frontier grant) - $1,600.000 to the MCIC. 2005 – 2008. Vanderknaap, E., Kamoun, S., Francis, D., Goodner, B. and Meulia, T. Instrumentation for High- throughput genotyping and sequencing projects. NSF-MUE. $285.000. June 1st 2004 – May 31st 2005. Jones, M.L., Meulia, T., and Streeter, J. Role of Metacaspases in petunia petal and leaf senescence. OARDC Interdisciplinaty Team Research Competition. 2005 - 2006. $100,000. Finer, J., Ling, P., and Meulia, T. High throughput digital image analysis for promoter analysis in transiently transformed plant tissues. OARDC - Interdisciplinary Team Research Grant Proposal. $100,000. 2003 –2005.

Synergistic Activities  In 1995 I was one of the founders of WAMBA (Wooster Area Molecular Biology Association) a discussion group, which includes plant and animal scientists from the OARDC, and from the nearby liberal art College of Wooster. This group still meets weekly holding seminars with invited speakers, or discussions with students, postdocs, faculty on various molecular biology and genomics topics.  I organize facility tours and science hands on demonstrations for local elementary, middle-high and high school students, and for students of the nearby Agricultural Technical Institute. In this year over 300 students toured and participated in demonstrations at the center.  Represent Wooster campus molecular and genomic researchers during State, University or Industry representatives visits at the OARDC. Some of this year visitors included: Community representative and politicians: Gordon Gee (OSU president), Joseph Alutto (OSU Provost), Ron Amstutz (Ohio State Senator), James Carmicheal (Ohio State Representative), Dave Baldwin (Ohio BioScience Consortium Coordinator), Christine Hazel (Dupont/Pioneer), Brad Moffit (Ohio Department of Ag.Education), a Delegation of Iraqi agricultural professionals.  Represent the OARDC at public events, such as Plant Discovery Day, BioOhio and the County Fair. Thomas K. Mitchell Fungal Biology and Parasitism Laboratory Department of Plant Pathology (614) 292-1728 The Ohio State University Fax: (614) 292-4455 Columbus, OH 43210 E-mail: [email protected]

(a) Professional Preparation Pennsylvania State University Plant Science B.S. 1992 Clemson University Plant Pathology M.S. 1995 North Carolina State University Plant Pathology Ph.D. 2000

(b) Appointments 2007-present Assistant Professor The Ohio State University, Department of Plant Pathology 2001-2007 Research Assistant Professor North Carolina State University, Center for Integrated Fungal Research 1999–2001 Senior Research Scientist North Carolina State University, Fungal Genomics Laboratory

(c) Publications (i) Related Publications Oh, Y. Y., Donofrio, N., Pan, H., Coughlan, S., Brown, D., Meng, S., Mitchell, T., and R. Dean (2008). Transcriptome analysis reveals new insight into appressorium formation and function in the rice blast fungus, Magnaporthe oryzae. Genome Biology 9:R85.

Craven, K.D., Valez, E., Hicks, R., Cho, Y., Lawrence C.B., and T. K. Mitchell, (2008). The role of anastomosis in pathogenicity of the fungal pathogen Alternaria brassicicola. Eukaryotic Cell 7:675-683.

Yangre, C., Cramer, R., Kwang-Hyung, K., Davis, J., Mitchell, T., Figuli, P., Prayor, B., Lemasters, E., and C. Lawrence, (2007). The Fus3/Kss1 MAP kinase homolog amk1 regulates the expression of several hydrolytic enzyme genes in the fungus Alternaria brassicicola. Fungal Genetics and Biology, 44:543- 553.

Torto-Alalibo, T., Tripathy, S., Smith, B.M., Arredondo, F., Zhou, L., Li, H., Qutob, D., Gijzen, M., Mao, C., Sobral, B.W.S., Waugh, M.E., Mitchell, T.K., Dean, R.A., and B. M. Tyler, (2007). Expressed sequence tags from Phytophthora sojae reveal genes specific to development and infection. Molecular Plant-Microbe Interactions, 20:781-793.

Jeong, J., Mitchell, T., and R. Dean, (2007). Characterization of the Magnaporthe grisea snodprot1 homolog, MgSPH1. FEMS Microbiology Letters. 273:157-165.

(ii) Significant Publications Donofrio, N., Oh, Y., Lundy, R., Pan, H., Jeong, J., Coughlan, S., Mitchell, T., Dean, R. (2006). Gene expression during nitrogen starvation in the rice blast fungus, Magnaporthe grisea. Fungal Genetics and Biology, 43:605-617.

Dean, R., Talbot, N., Ebbole, D., Farman, M., Mitchell, T., Orbach, M., Thon, M., Kulkarni, R., Xu, J-R., Huaqin, P., Read, N., Lee, Y-H., Carbone, I., Brown, D., Soanes, D., Djonovic, S., Kolomiets, E., Rehmeyer, C., Li, W., Harding, M., Lebrun, M-H., Bohnert, H., Kim, S., Galagan, J., Birren, B., (2005). Analysis of the genome sequence of the plant pathogenic fungus Magnaporthe grisea, the causal agent of rice blast disease. Nature 434:980-986. Mitchell, T.K., Alejos-Gonzalez, F., Gracz, H.S., Danehower, D.A., Daub, M.E., Chilton, W.S. (2003). Xanosporic acid, an intermediate in bacterial degradation of the fungal phototoxin cercosporin. Phytochemistry 62:723-732.

Mitchell, T.K., Thon, M.R., Jeong, J-S., Brown, D.E., Deng, J., Dean, R.A. (2003). The rice blast pathosystem as a case study for the development of new tools and raw materials for genome analysis of fungal plant pathogens. New Phytologist 159: 53-61.

Zhu, H., Bilgin, M., Bangham, R., Hall, D., Casamayor, A., Bertone, P., Lan, N., Jansen, R., Bidlingmaier, S., Houfek, T., Mitchell, T.K., Miller, P., Dean, R., Gerstein, M., and Snyder, M. (2002). Global analysis of protein activities using proteome chips. Science 293:2101-2105.

(d) Synergistic Activities In addition to research activities, my work has extended beyond the laboratory. These extensions include the participation in the development of extensive database (MGOS) housing the genomic and proteomic data and bioinformatic tools for the rice blast pathogen M. oryzae. Additionally, I have worked to design a LIMs system for the high throughput functional analysis of over 50,000 random insertion mutants. My efforts in the area of outreach and engagement are extensive and varied. Over the past 8 years I developed a laboratory manual on genetics and genomics and distributed over 40,000 copies, trained over 60 high school teachers in genomics at weeklong workshops, gave talks and laboratory exercises to over 30 K-12 classes, mentored teachers and high school students through research projects, to name a few. With regard to engagement to minority colleges and students, I have developed course sections and grants in collaboration with HBCU institutions, mentored faculty on research projects, give dozens of talks and seminars, and aggressively recruited minority students into the STEM disciplines.

(e) Collaborator and Other Affiliations (i) Collaborators and co-editors - F. Arredondo Virginia Bioinformatics Institute, B. Birren Broad Institute, D. Brown North Carolina State University, I. Carbone North Carolina State University, Y. Cho University of Hawaii, R. Cramer Montana State University, S. Coughlan, Agilent, N. Donofrio University of Delaware, D. Ebbole Texas A&M University, M. Farman University of Kentucky, J. Galagan Broad Institute, M. Gijzen Wageningen University Netherland, L. Kohn University of Toronto, C.B. Lawrence Virginia Bioinformatics Institute, M-H. Lebrun Beyer Crop Science France, Y-H. Lee Seoul National University Korea , H. Li Virginia Bioinformatics Institute, L. J. Ma Broad Institute, S. Marek Oklahoma State University, C. Mao Virginia Bioinformatics Institute, D. Okabara University of Washington, M. Orbach University of Arizona, B. Prayor University of Arizona, D. Qutob Virginia Bioinformatics Institute, N. Read University of Edinburgh Scotland, B.M. Smith Virginia Bioinformatics Institute, B.W.S. Sobral Virginia Bioinformatics Institute, C. Soderlund University of Arizona, N. Talbot University of Exeter UK, M. Thon Salamanca University Spain, T. Torto-Alalibo Virginia Bioinformatics Institute, S. Tripathy Virginia Bioinformatics Institute, B. M. Tyler Virginia Bioinformatics Institute, B. Valent Kansas State University, N. Walker Oklahoma State University, G.L. Wang Ohio State University, J.R. Xu Purdue University, Y. Yang Penn State University, L. Zhou Virginia Bioinformatics Institute, h. Zhu John’s Hopkins School of Medicine

(ii) Graduate advisors - M. Daub North Carolina State University, R. Dean North Carolina State University, G. Payne North Carolina State University

(iii) Thesis and postdoctoral advisees - K. Craven Nobel Foundation, E. Valez North Carolina State University, S. Kim Ohio State University

BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Randy Scholl Associate Professor, Interim Chair eRA COMMONS USER NAME

University of Illinois B.S. 1968 Agricultural Science North Carolina State Univ. M.Sc. 1970 Crop Science North Carolina State Univ. Ph.D. 1972 Crop Science

A. Positions and Honors.

Positions and Employment 1968-1972 Research Associate, North Carolina State Univ., Raleigh 1972-1973 Post-doctoral Associate, Univ. of Illinois, Urbana 1972-1978 Assistant Professor, Dept. of Genetics, The Ohio State Univ., Columbus 1978-1987 Associate Professor, Dept. of Genetics, The Ohio State Univ., Columbus 1987-1998 Associate Professor, Dept. of Molecular Genetics, The Ohio State Univ., Columbus 1998-present Associate Professor, Dept. of PCMB, The Ohio State Univ., Columbus

Other Experience and Professional Memberships 1986 Visiting Faculty, DOE Plant Res. Lab., Michigan State Univ., East Lansing 1990-present Director, Arabidopsis Biological Resource Center, Ohio State 2001-2006 Executive Committee, TAIR database 2006-present Interim Chair, Dept. of Plant Cellular & Molecular Biology, Ohio State Univ.

B. Selected peer-reviewed publications (in chronological order).

Scholl, R. L., J. E. Harper and R. H. Hageman. 1974. Improvement of the nitrate color development in assays of nitrate reductase by phenazine methosulfate and zinc acetate. Plant Physiol. 53:825-828. Scholl, R. L. 1974. The inheritance of isocitratase activity and its relationship to seedling vigor in a cross of upland cotton (Gossypium hirsutum L.). Crop Sci. 14:296-300. Scholl, R. L. 1976. Variability within Gossypium hirsutum L. for seedling isocitratase activity. Crop Sci. 16:710-703. Scholl R. L. and P. A. Miller. 1976. Genetic association between yield and lint quality in cotton. Crop Sci. 16:780-783. Amos, J. A. and R. L. Scholl. 1977. Effect of growth temperature on leaf nitrate reductase, glutamine synthetase, and NADH-glutatamate dehydrogenase of juvenile maize genotypes. Crop Sci. 17: 445-448. Scholl, R. L. and K. M. Dennison. 1978. Sensitivity of cultured tissue of Arabidopsis thaliana races to sulfanilamde. Physiol. Plantar. 43:321-325. Amos, J. A. and R. L. Scholl. 1978. Induction of haploid callus from anthers of four species of Arabidopsis. Z. Ppflanzenphysiol. 90:33-43. Sharma, R. K., B. Griffing and R. L. Scholl. 1979. Variation among Arabidopsis thaliana L. (Heynh.) races for survival in limited carbon dioxide. Theor. Appl. Genet. 54: 11-15. Scholl, R. L. and J. A. Amos. 1980. Isolation of doubled haploid plants through anther culture in Arabidopsis thaliana. Z. Pflanzenphysiol. 96:407-414. Scholl, R. L., D. E. Keathley and T. J. Baribault. 1981. Enhancement of root formation and fertility in shoots regenerated from anther- and seedling-derived callus cultures of Arabidopsis thaliana. Z. Pflanzenphysiol. 104:225-231. Keathley, D. E. and R. L. Scholl. 1982. Culture of Arabidopsis thaliana anthers on liquid medium. Z. Pflanzenphysiol. 106:199-212. Keathley, D. E. and R. L. Scholl. 1983. Chromosomal heterogeneity of Arabidopsis thaliana anther callus, regenerated shoots and plants. Z. Pflanzenphysiol. 112:247-255. Wang, X. -M., R. L. Scholl and K. A. Feldmann. 1986. Characterization of a chlorate-hypersensitive, high nitrate reductase Arabidopsis thaliana mutant. Theor. Appl. Genet. 72:328-336. Zhang, H., R. L. Scholl and C. Somerville. 1988. Double stranded DNA sequencing as a choice for DNA sequencing. Nucleic Acids Res. 16: 1220. Wang, X., K. A. Feldmann, and R. L. Scholl. 1988. A chlorate-hypersensitive, high nitrate; chlorate uptake mutant of Arabidopsis thaliana. Physiol. Plantar. 73: 305-310. Kim, Y., H. Zhang and R.L. Scholl. 1990. Two evolutionarily divergent genes encode a cytoplasmic ribosomal protein of Arabidopsis thaliana. Gene 92:177-182. You T. H., and Scholl, R. L. 1998. PCR amplification of cDNA Libraries forCloning and Screening. BioTechniques 24:574-575. Garcia-Hernandez M, Berardini TZ, Chen G, Crist D, Doyle A, Huala E, Knee E, Lambrecht M, Miller N, Mueller LA, Mundodi S, Reiser L, Rhee SY, Scholl R, Tacklind J, Weems DC, Wu Y, Xu I, Yoo D, Yoon J, Zhang P. 2002. TAIR: a resource for integrated Arabidopsis data. Funct Integr Genomics. Nov; 2(6): 239-53. Griffing, B. and R.L. Scholl. l991. Qualitative and quantitative studies of Arabidopsis thaliana. Genetics 129:605-609. Scholl, R. L, Ware D. H. and May, S. 2000. Seed and Molecular Resources for Arabidopsis. Plant Physiol. 2000 124: 1477-1480. Meinke, D. and Scholl. R. 2003. The Preservation of Plant Genetic Resources. Experiences with Arabidopsis. Plant Physiol. 133: 1046-1050. Rivero-Lepinckas, Luz, Crist, Deborah, Scholl, Randy 2006. Growth of plants and preservation of seeds. METHODS IN MOLECULAR BIOLOGY 323:3-12.

C. Research Support

Ongoing Research Support

DBI-0542034 Scholl (PI) 5/01/06-3/31/2011 NSF The Arabidopsis Biological Resource Center at The Ohio State University ($2,940,000) Role: PI

Completed Research Support

DBI-0091471-suppl. Scholl (PI) 8/2003-3/2006 NSF The Arabidopsis Biological Resource Center at The Ohio State University ($240,000) Role: PI

DBI-0091471 Scholl (PI) 4/2001-3/2006 NSF The Arabidopsis Biological Resource Center at The Ohio State University ($2,790,000) Role: PI IBN-9600964-suppl. Scholl (PI) 2/2000-2/2001 NSF The Arabidopsis Biological Resource Center at The Ohio State University ($50,000) Role: PI

IBN-9600964 Scholl (PI) 3/1997-2/2001 NSF The Arabidopsis Biological Resource Center at The Ohio State University ($1,856,000) Role: PI

BIR-9113224 Scholl (PI) 9/1996-1/1997 NSF - extension The Arabidopsis Biological Resource Center at The Ohio State University ($179,000) Role: PI

BIR-9113224 Scholl (PI) 9/1991-8/1996 NSF The Arabidopsis Biological Resource Center at The Ohio State University ($1,940,000) Role: PI

? Scholl (PI) 1979-82 NSF Anther Culture in Arabidopsis ($58,000)

BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE David E. Somers Associate Professor eRA COMMONS USER NAME

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) Ohio University, Athens, OH B.Sc. 1973-1977 Botany University of Alberta, Edmonton, Alberta Canada. M.Sc 1977-1981 Physiological Plant Ecology Ph.D. 1987-1994 Plant Biology University of California at Berkeley, Berkeley The Scripps Research Institute, La Jolla, CA Post-doctoral 1994-1999 Plant Mol. Genetics

A. Positions and Honors.

Positions and Employment 1977-1980 Graduate Teaching Assistant. Dept. of Botany, University of Alberta, Edmonton, Alberta, Canada. 1981-1982 Research Assistant, Dept. of Botany, University of Washington, Seattle, WA. 1985-1987 Research Technician, Howard Hughes Medical Institute. University of Washington, Seattle, WA. 1987-1994 Graduate Research Assistant, Dept. of Plant Biology, UC-Berkeley, Berkeley, CA. Peter Quail, advisor. 1994-1999 Postdoctoral Research Fellow, Dept. of Cell Biology, The Scripps Research Institute, La Jolla, CA. Steve A. Kay, advisor. 1999-2005 Assistant Professor, Department of Plant Biology, The Ohio State University, Columbus OH 2005-present Associate Professor, Department of Plant Biology, The Ohio State University, Columbus OH

Other Experience and Professional Memberships Member: Society for Research on Biological Rhythms Member: American Society of Plant Biologists Grant review panels 2005 National Science Foundation, Integrative Plant Biology Panel 2005-2006 National Institute of Health, CSD Study Section 2006 National Institute of Health, NRSA Cell Biology and Development (F05) Study Section 2007-2008 National Institute of Health, Neurogenesis and Cell Fate Study Section, Cellular Signaling and Regulatory Systems Ad hoc reviews 2000-present NSF, USDA, BBSRC (UK), BSF (US-Israel Binational Science Foundation), Marsden Fund (The Royal Society of New Zealand) Journal Peer review Science, Nature, PNAS, Current Biology, Nature Reviews Microbiology, PLoS Biology, The Plant Cell, Plant Journal, Plant Physiology, Plant Molecular Biology, BMC Plant Biology , Planta, Cell Research, Journal of Biological Rhythms, Plant and Cell Physiology, Philos Trans R Soc Lond B Biol Sci., Plant Cell and Environment, Photochemistry and Photobiology, Journal of Phycology

1 Honors 1997 JSPS Short-Term Invitation Fellow, Hitachi Advanced Research Lab, Saitama, Japan. 1994-1997 NSF Postdoctoral Research Fellow in Plant Biology

B. Selected peer-reviewed publications (in chronological order; selected from 30 peer- reviewed publications). 1. Somers, D.E., Sharrock, R.A., Tepperman, J.T., and Quail, P.H. 1991. The hy3 long hypocotyl mutant of Arabidopsis is deficient in phytochrome B. Plant Cell 3:1263-1274. 2. Devlin, P. F., Rood, S., Somers, D.E., Quail, P.H., and Whitelam, G.C. 1992. Photophysiology of the elongated internode (ein) mutant of Brassica rapa: The ein mutant lacks a detectable phytochrome B-like polypeptide. Plant Physiol 100:1442-1447. 3. Wester, L., Somers, D.E., Clack, T. and Sharrock, R.A. 1993. Transgenic complementation of the hy3 phytochrome B mutation and PHYB gene copy number effects in Arabidopsis. Plant J. 5:261-272 4. Dehesh, K., Franci, C., Sharrock, R.A., Somers, D.E., Welsh J. and Quail, P.H. 1994. The Arabidopsis phytochrome A gene has multiple transcription start sites and a promoter sequence motif homologous to the repressor element of monocot phytochrome A genes. Photochem. Photobiol. 59: 379-384. 5. Somers, D.E. and Quail, P.H. 1995. Phytochrome-mediated light regulation of PHYA- and PHYB-GUS transgenes in Arabidopsis thaliana seedlings. Plant Physiol 107:523-534. 6. Somers, D.E. and Quail, P.H. 1995. Temporal and spatial expression patterns of PHYA and PHYB genes in Arabidopsis. Plant J. 7:413-427 7. Hicks, K.A., Millar, A.J., Carre, I.A., Somers, D.E., Straume, M., Meeks-Wagner, D.R. and Kay, S.A. 1996. Conditional circadian dysfunction of the Arabidopsis early-flowering 3 mutant. Science 274: 790-792. 8. Anderson, S.L., Somers, D.E., Millar, A.J., Hanson, K., Chory, J. and Kay, S.A. 1997. Attenuation of phytochrome A and B signaling pathways by the Arabidopsis circadian clock. Plant Cell 9:1727-1743. 9. Devlin, P.F., Somers, D.E., Quail, P.H., and Whitelam, G.C. 1997. The Brassica rapa elongated internode (EIN) gene encodes phytochrome B. Plant Mol. Biol. 34:537-547. 10. Somers, D.E., Devlin, P.A. and Kay, S.A. 1998. Phytochromes and cryptochromes in the entrainment of the Arabidopsis circadian clock. Science 282:1488-1490. 11. Somers, D.E., Webb, A., Pearson, M., and Kay, S.A. 1998. The short-period mutant, toc1-1, alters circadian clock regulation of multiple outputs throughout development in Arabidopsis thaliana. Development 125:485-494. 12. Park D.H.*, Somers D.E.*, Kim Y.S., Choy Y.H., Lim H.K., Soh M.S., Kim H.J., Kay S.A., Nam H.G. 1999. Control of circadian rhythms and photoperiodic flowering by the Arabidopsis GIGANTEA gene. Science 285: 1579-1582. *(equal contributions as first authors). 13. Somers, D.E., Schultz, T.F., Milnamow, M. and Kay S.A. 2000. ZEITLUPE encodes a novel clock associated PAS protein from Arabidopsis. Cell 101:319-329. 14. Strayer, C., Oyama, T., Schultz, T.F., Raman, R., Somers, D.E., Mas, P., Panda, S., Kreps, J.A.and Kay, S.A. 2000. Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response regulator homolog. Science 289:768-771. 15. Kim, W-Y.*, Geng, R.* and Somers, D.E. 2003. Circadian phase-specific degradation of the F- box protein ZTL is mediated by the proteasome. Proc Natl Acad Sci U S A 100:4933-4938. *(equal contributions as first authors). 16. Risseeuw, E.P., Daskalchuk, T.E., Banks, T.W., Liu, E., Cotelesage, J., Hellman, H., Estelle, M., Somers, D.E., and Crosby, W.L. 2003. Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis. Plant J. 34:753-767. 17. Mas, P., Kim, W-Y., Somers D.E., and Kay S.A. 2003. Targeted degradation of TOC1 by ZTL modulates circadian function in Arabidopsis thaliana. Nature 426:567-70. 18. Somers, D.E., Kim, W-Y., Geng, R. 2004. Dosage-dependent control of circadian period 2 and photomorphogenesis by the F-box protein ZEITLUPE. Plant Cell 16:769-782. 19. Han, L., Mason, M, Risseeuw, E.P., Crosby, W.L. and Somers, D.E. 2004. Formation of an SCFZTL complex is required for proper regulation of circadian timing. Plant J. 40:291-301. 20. Kim, W.Y., Hicks, K. A. and Somers, D.E. 2005. Independent roles for EARLY FLOWERING 3 and ZEITLUPE in the control of circadian timing, hypocotyl length, and flowering time. Plant Physiol. 139: 1557-69. 21. Kevei, E., Gyula, P., Hall, A., Kozma-Bognar, L., Kim, W.Y., Eriksson, M.E., Toth, R., Hanano, S., Feher, B., Southern, M.M., Bastow, R.M., Viczian, A., Hibberd, V., Davis, S.J., Somers, D.E., Nagy, F., and Millar, A.J. 2006. Forward genetic analysis of the circadian clock separates the multiple functions of ZEITLUPE. Plant Physiol. 140:933-945. 22. Allen T., Koustenis A., Theodorou G., Somers, D.E., Kay S.A., Whitelam G.C., Devlin P.F. 2006. Arabidopsis FHY3 specifically gates phytochrome signaling to the circadian clock. Plant Cell 18:2506-2516 23. Kim,W.Y.*, Fujiwara, S.*, Suh, S.S., Kim, J., Kim, Y., Han, L., David, K., Putterill, J., Nam, H.G., and Somers, D.E. 2007. ZEITLUPE is a circadian photoreceptor stabilized by GIGANTEA in blue light. Nature 449:356-360. *(equal contributions as first authors). 24. Jin JB, Jin YH, Lee J, Miura K, Yoo CY, Kim WY, Van Oosten M, Hyun Y, Somers DE, Lee I, Yun DJ, Bressan RA, Hasegawa PM. 2008. The SUMO E3 ligase, AtSIZ1, regulates flowering by controlling a salicylic acid-mediated floral promotion pathway and through effects on FLC chromatin structure. Plant J. 3:530-40 25. Fujiwara, S.*, Wang, L.*, Han, L., Suh, S. S., Salome, P. A., McClung, C. R., Somers, D.E. 2008. Post-translational regulation of the Arabidopsis circadian clock through selective proteolysis and phosphorylation of pseudo- response regulator proteins. J. Biol. Chem. 283: 23073-23083. *(equal contributions as first authors). 26. Somers, D. E. and Fujiwara, S. 2009. Thinking outside the F-box: novel ligands for novel receptors. Trends Plant Sci. (in submission).

C. Research Support Ongoing Research Support

NSF Molecular and Cellular Biosciences 05/01/2006 - 04/30/2009 MCB-0544137 Somers (PI) Functional Domains and Spatial-Temporal Interactions of an Arabidopsis Circadian Clock-Associated F-Box Protein”. This study focuses on the functional dissection of ZTL, its intracellular site of action, and identifies additional biochemical interactors.

NSF Integrative Organismal Biology 02/01/2008 - 01/31/2011 IOS-0748749 Somers (PI) The control of reproductive onset and the circadian clock by GIGANTEA. This project defines the biochemical function of GI by identifying which domains are effective for the interaction and stabilization of ZTL and identifies other GI interactors.

Completed Research Support NSF Molecular and Cellular Biosciences 04/01/2001 - 03/31/2004 MCB-0080090 Somers (PI) Molecular and genetic analysis of ZTL function in the Arabidopsis circadian clock”. This work positioned the ZTL locus within the context of the known red light and blue light signal transduction pathways and identified physical interaction partners of the ZTL protein.

3 CSREES/USDA Plant Growth and Development 09/01/2002 - 08/31/2004 2002-35304-12594 Somers (PI) Functional in planta analyses of a clock-associated F-box protein. The goals of this project was to identify and confirm the functional domains of ZTL.

NSF Integrative and Organismal Biology: 04/01/2004 - 03/31/2007 IBN-0344377 Somers (PI) Genetic and molecular interactors in circadian clock function. The major goals of this project were to identify genetic suppressors and enhancers of the circadian clock component ZEITLUPE.

NSF Biological Infrastructure 05/01/2005 - 04/30/2008 DBI-0500601 Somers (lead PI) High-resolution CCD camera and luminometer for high-throughput luciferase-based promoter analysis. This award supported the purchase of two systems used to localize and measure luciferase activity resulting from expression of chimeric reporter genes that consist of the luciferase gene fused to a promoter region of the genome. One system is a high throughput luminometer and the other is based on a low-light CCD camera.

4 BIOGRAPHICAL SKETCH Eric J. Stockinger Department of Horticulture and Crop Science The Ohio State University/OARDC Wooster OH 44691

Professional Preparation University of California, Riverside CA Plant Science B.S. 1985 University of California, Riverside CA Botany (Plant Genetics) Ph.D. 1993 Michigan State University, East Lansing MI Plant Genetics Postdoc 1993-1994 Michigan State University, East Lansing MI Gene Regulation Postdoc 1994-2000

Appointments 2006-Present Associate Professor, Dept of Horticulture and Crop Science. The Ohio State University/OARDC, Wooster OH 44691 2000-2006 Assistant Professor, Dept of Horticulture and Crop Science. The Ohio State University/OARDC, Wooster OH 44691 1994-2000 Postdoctoral Research Assistant, Dept of Crop & Soil Sciences, Michigan State University, East Lansing MI; Research Advisor: Michael F. Thomashow 1993-1994 Postdoctoral Research Assistant, Dept of Horticulture, Michigan State University, East Lansing MI; Research Advisor: Amy F. Iezzoni 1986-1993 Research Assistant, Dept of Botany & Plant Sciences, University of California Riverside CA; Research Advisor: Linda L. Walling 1990 Teaching Assistant (Molecular Biology), Dept of Biology, University of California, Riverside CA; Teaching Advisor: Brad Hyman 1986 Teaching Assistant (Genetics), Dept of Biology, University of California, Riverside CA; Teaching Advisor: Gary Jones 1984-1986 Undergraduate Research Assistant, Dept of Botany & Plant Sciences, University of California, Riverside CA; Research Advisor: J. Giles Waines

Publications: Pennycooke, J.C., Cheng, H., Roberts, S.M., Yang, Q., Rhee, S.Y., and Stockinger, E.J. (2008) The low temperature-responsive, Solanum CBF1 genes maintain high identity in their upstream regions in a genomic environment undergoing gene duplications, deletions, and rearrangements. Plant Mol. Biol. 67, 483-497. Pennycooke, J.C., Cheng, H. and Stockinger, E.J. (2008) Comparative genomic sequence and expression analyses of Medicago truncatula and alfalfa subspecies falcata COLD-ACCLIMATION-SPECIFIC genes. Plant Physiol. 146, 1242-1254. Knox, A.K., Li, C., Vagujfalvi, A., Galiba, G., Stockinger, E.J., and Dubcovsky, J. (2008) Identification of candidate CBF genes for the frost tolerance locus Fr-Am2 in Triticum monococcum. Plant Mol Biol. 67, 257-270. Xiao, H., Jiang, N., Schaffner, E., Stockinger, E.J., and van der Knaap, E. (2008) A retrotransposon- mediated gene duplication underlies morphological variation of tomato fruit. Science 319, 1527-1530. Stockinger, E.J., Skinner, J.S., Gardner, K.G., Francia, E., and Pecchioni, N. (2007) Expression levels of barley Cbf genes at Frost resistance-H2 are dependent upon alleles at Fr-H1 and Fr-H2. Plant J. 51:308- 321. Stockinger, E.J., Cheng, H., and Skinner, J.S. (2005) Structural organization of barley CBF genes coincident with a QTL for cold hardiness. In, “Cold Hardiness in Plants,” THH Chen, M Uemura and S Fujikawa, (eds.), CABI Publishing, Wallingford, Oxon, UK. pp. 53-63. Wang, Z., Triezenberg, S.J., Thomashow, M.F., and Stockinger E.J. (2005) Multiple hydrophobic motifs in Arabidopsis CBF1 COOH-terminus provide functional redundancy in trans-activation. Plant Mol. Biol. 58:543-559. Stockinger, E.J., Mao, Y., Regier, M., Triezenberg, S.J. and Thomashow, M.F. (2001) Transcriptional Adaptor and Histone Acetyltransferase Proteins in Arabidopsis and their Interactions with CBF1, a Transcriptional Activator Involved in Cold-Regulated Gene Expression. Nucleic Acids Res. 29:1524-1533. Stockinger, E.J., Gilmour, S.J. and Thomashow, M.F. (1997) Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc. Natl. Acad. Sci. U.S.A. 94:1035-1040. Manuscripts Submitted: Knox, A.K., Cheng, Hongmei, Tondelli, A., Dhillon, T., Nelson, C.W., Welch, L.R., Pecchioni, N., and Stockinger, E.J. (Submitted) The barley (Hordeum vulgare) CBF2A-CBF4B genomic region at Frost Resistance-H2 exists as a copy number variant locus in which multiple gene copies typify cultivated genotypes possessing vrn-H1 winter alleles

Synergistic Activities • Established summer internship program bringing students from underrepresented groups to the Stockinger lab (seven students 2002 to 2005); training of eight additional undergraduate students in research • Co-instructor of Agricultural genomics; Courses developed: 1) Yeast One-Hybrid, a laboratory exercise; 2)Transcription factors controlling plant traits of agroeconomic value; 3) Molecular Genetic Control of Flowering: Integration of Environmental Signals with the Endogenous Program of Plant Development • Developed online handbook of molecular biology protocols (http://www.oardc.ohio- state.edu/stockingerlab/) • Created bacteriophage λ genomic libraries (available upon request) in 16 plant genotypes and species • Journal peer review service (40 articles 2000-2008; Biochim. Biophys. Acta., Genetics, J. Biol. Chem., Int. J. Biochem. Cell Biol., J. Am Soc. Hort Sci., J. Exp Bot., Mol. Genet. Genomics, Nucleic Acids Res., Plant Cell, Plant J., J. Plant Res., Plant Breeding, Plant Mol. Biol., Plant Physiol., Proc. Natl. Acad. Sci., U.S.A., Science) • Grant review service (Ad hoc reviewer 12 proposals 2000-2008; NSF, USDA NRI, U.S. Israel BARD, and internal, and Panel Member 2006 USDA NRI Biographical Sketch F. Robert Tabita, Principal Investigator

Education: B.S. in Biology/Chemistry (1961-65) and M.S. in Microbiology (1965-67), St. John’s University, Jamaica, N. Y.; Ph.D. in Microbiology/Biochemistry (1967-71), Syracuse University (with D.G. Lundgren); NIH Postdoctoral Fellow in Department of Chemistry (1971-73), Washington State University (with B. A. McFadden).

Positions: Professional Experience: 1973-1988, Assistant, Associate, and Professor, Department of Microbiology, University of Texas at Austin; 1985, Visiting Professor, Summer program at Gray Fresh-water Res. Inst., Univ. of Minnesota; 1981-1988, Associate Director, Center for Applied Microbiology, University of Texas at Austin; 1987, Sabbatical in protein engineering at Genencor/Genentech, Inc., South San Francisco; 1989-Present, Ohio Eminent Scholar of Microbiology; Professor, Dept. of Microbiology, Prof. Plant Cellular and Molecular Biology Dept., Prof. Natural Resources, The Ohio State University; member of interdisciplinary Biochemistry and Molecular Biology programs; Chair, OSU Provost's Initiative in the Molecular Life Sciences (1997-2002); Currently Director, OSU Plant Molecular Biology/Biotechnology Program and Plant Biotechnology Center; Director, OSU Plant-Microbe Genomics Facility

Honors and Professional Service: 1961-65, N.Y. State Regents Scholarship (undergraduate); 1971-73, NIH Postdoct. Fellowship; 1976-78, Member NIH Biochemistry Study Section; 1979- 84, Member Ed. Bd. J. Bacteriology; 1981, Member USDA Biological N2 Fixation Review Panel; 1984, Member of USDA Photosynthesis Panel; 1984-88, Member of NIH Microbial Physiology & Genetics Study Section (subcom.1); 1986, Co Editor, FEMS Microbiol. Rev. Special Edition on C1 Metabolism; 1989, Appointed Visiting Prof. and external Ph.D. examiner (opponent) at Uppsala Univ.; 1990, Co-Editor, Advances in Microbial Autotrophy and One-Carbon Metabolism (vol. 1) Kluwer Acad. Pub.; 1992, Member NIH Biochemistry Study Section for AREA Grants; 1993, Member Special NIH Study Section to evaluate grants of study section members; 1994, Elected Fellow of the Amer. Acad. Microbiol.; 1994-1996, Alt. Councilor, then Councilor, Microbial Physiol.& Metab. Section of Amer. Soc. Microbiol.; 1994, Chosen to nominate Nobel Prize in Chemistry by Nobel Comm.; 1995-2000, Editor, Arch. Microbiol.; 1996, Chair, Special Study Section for NIH to evaluate grants of study section members; 1996, Member of Eval. Team for Biology Dept. at Univ. Memphis; 1995, Co-Ed., Microbial Growth on C1 Compounds, Kluwer, 363 pp.; 1997-1998, Chair and Founder, Gordon Research Conference "Molecular Basis of Microbial One-Carbon Metabolism"; 1998, Member of External Committee to Evaluate Functional Genomics/Bioinformatics Res. Center at Virginia Polytechnic Institute and State University; 2000, DOE Energy Biosciences Grants Review Panel; 2001, DOE Advis. Comm. on Carbon Sequestration; 2002, Member DOE Genomes to Life Review Panel; 2005, Subpanel Chair (Biofuels), DOE Solar Energy Conf.; 2005, DOE Energy from Biomass Workshop; 2005 - present, Scientific Advisory Comm., Center for Molecular Systems, Oak Ridge National Lab

Publications since 2003 (of a total of 210)

179. Robinson, J. J., Scott, K. T., Swanson, S. T., O'Leary, M. H., Horken, K., Tabita, F. R, and Cavanaugh, C. M. Kinetic isotope effect and characterization of form II RubisCO from the chemoautotrophic endosymbionts of the hydrothermal vent tubeworm Riftia pachyptila. Limnol. Oceanogr. 48 (2003) 48-54. 180. Dubbs, J. M., and Tabita, F. R. Interactions of the cbbII promoter-operator region with CbbR and RegA(PrrA) regulators indicate distinct mechanisms to control expression of the two cbb operons of Rhodobacter sphaeroides. J. Biol. Chem. 278 (2003) 16443- 16450.

1 181. Finn, M. W., and Tabita, F. R. Synthesis of catalytically active form III ribulose 1,5- bisphosphate carboxylase/oxygenase in archaea. J. Bacteriol. 185 (2003) 3049-3059. 182. Hanson, T. E., and Tabita, F. R. Insights into the stress response and sulfur metabolism revealed by proteome analysis of a Chlorobium tepidum mutant lacking the RubisCO- like protein. Photosynth. Res. 78 (2003) 231-248. 183. Smith, S. A., and Tabita, F. R. Positive and negative bioselection of mutant forms of prokaryotic (cyanobacterial) ribulose-1, 5-bisphosphate carboxylase/oxygenase. J. Mol. Biol. 331 (2003) 557-569. 184. Tabita, F. R. Research on carbon dioxide fixation in photosynthetic microorganisms (1971 – present). Photosynth. Res. 80 (2004) 315-332. 185. Dubbs, J. M., and Tabita, F. R. Regulators of nonsulfur purple phototrophic bacteria and the interactive control of CO2 assimilation, nitrogen fixation, hydrogen metabolism and energy generation. FEMS Microbiol. Rev. 28 (2004) 353-376. 186. Larimer, F.W., Chain, P., Hauser, L., Lamerdin, J., Malfatti, S., Do, L., Land, M.,Pelletier, D.A., Beatty, J.T., Lang, A.S., Tabita, F. R., Gibson, J.L., Hanson, T. E., Bobst, C.,Torres y Torres, J., Peres, C., Harrison,F.H., Gibson, J., and Harwood, C.S. Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris. Nature Biotechnology 22 (2004) 55-61. [cover photograph]. 187. Tabita, F. R., and Hanson, T. E. Anoxygenic photosynthetic bacteria. In: Microbial Genomics (2004) C. M. Fraser, K. E. Nelson, and T. D. Read (eds.). Humana Press, Inc., Totowa, NJ, pp. 225-243. 188. Bobst, C. E., and Tabita, F. R. The role of cysteine 160 in thiamine diphosphate binding of the Calvin-Benson-Bassham cycle transketolase (CbbT) of Rhodobacter sphaeroides. Arch. Biochem. Biophys. 426 (2004) 43-54. [cover photograph]. 189. Smith, S. A., and Tabita, F. R. Glycine 176 affects catalytic properties and stability of the Synechococcus sp. strain PCC 6301 ribulose 1,5-bisphosphate carboxylase/oxygenase. J. Biol. Chem. 279 (2004) 25632-25637. 190. Finn, M. W., and Tabita, F. R. A modified pathway to synthesize ribulose 1,5- bisphosphate in methanogenic archaea. J. Bacteriol. 186 (2004) 6360-6366. 191. Dubbs, P., Dubbs, J. M., and Tabita, F. R. Effector-mediated interaction of CbbRI and CbbRII regulators with target sequences in Rhodobacter capsulatus. J. Bacteriol. 186 (2004) 8026-8035. 192. Li, H., Sawaya, M. R., Tabita, F. R., and Eisenberg, D. Crystal structure of a novel RuBisCO-like protein from the green sulfur bacterium Chlorobium tepidum. Structure 13 (2005) 779-789. 193. Dangel, A. W., Gibson, J. L., Janssen, A. P., and Tabita, F. R. Residues that influence in vivo and in vitro CbbR function in Rhodobacter sphaeroides and identification of a specific region critical for co-inducer recognition. Mol. Microbiol. 57 (2005) 1397-1414. 194. Zianni, M.,Tessanne, K., Merighi, M., Laguna, R., and Tabita, F. R. Identification of the DNA bases of a DNase I footprint by the use of dye primer DNA sequencing on an automated capillary DNA analysis instrument. J. Biomol. Techniques 17 (2006) 103-113. 195. Romagnoli, S., and Tabita, F. R. A novel three-protein two-component system provides a regulatory twist on an established circuit to modulate expression of the cbbI region of Rhodopseudomonas palustris CGA010. J. Bacteriol. 188 (2006) 2780-2791. 196. VerBerkmoes, N. C., Shah, M. B., Lankford, P. K., Pelletier, D. A., Strader, M. B., Tabb, D. L., McDonald, W. H., Barton, J. W., Hurst, G. B., Hauser, L., Davison, B. H., Beatty, J. T., Harwood, C. S., Tabita, F. R., Hettich, R. L., and Larimer, F. W. Determination and comparison of the baseline proteomes of the versatile microbe Rhodopseudomonas palustris under its major metabolic states. J. Proteome Res. 5 (2006) 287-298.

2 197. John, D. E., Wawrik, B., Tabita, F. R., and Paul, J. H. Gene diversity and organization in rbcL-containing genome fragments from uncultivated Synechococcus in the Gulf of Mexico. Mar. Ecol. Prog. Ser. 316 (2006) 23-33. 198. Tabita, F. R. Research on carbon dioxide fixation in photosynthetic microorganisms (1971-present) In: Discoveries in Photosynthesis (2006). Govindjee, J. T. Beatty, H. Gest, and J. F. Allen (eds.). Springer, pp. 771-788. 199. Kim, W., and Tabita, F. R. Both subunits of ATP-citrate lyase from Chlorobium tepidum contribute to catalytic activity. J. Bacteriol. 188 (2006) 6544-6552. 200. Hoeft, S. E., Switzer-Blum, J., Stolz, J. F., Tabita, F. R., Witte, B., King, G. M., Santini, J. M., and Oremland, R.S. Alcalilimnicola ehrlichei, sp. nov., a novel, arsenite-oxidizing haloalkaliphilic proteobacterium capable of chemoautotrophic or heterotrophic growth with nitrate or oxygen as the electron acceptor. Int. J. Syst. & Evol. Microbiol. 57 (2007) 504-512. 201. Romagnoli, S., and Tabita, F. R. Phosphotransfer reactions of the CbbRRS three-protein two-component system from Rhodopseudomonas palustris CGA010 appear to be controlled by an internal molecular switch on the sensor kinase. J. Bacteriol. 189 (2007) 325-335. 202. Kreel, N. E., and Tabita, F. R. Substitutions at methionine 295 of the Archaeoglobus fulgidus ribulose-1,5-bisphosphate carboxylase/oxygenase affect oxygen binding and CO2/O2 specificity. J. Biol. Chem. 282 (2007) 1341-1351. 203. Tabita, F. R. Rubisco: the enzyme that keeps on giving. Cell 129 (2007) 1039-1040. 204. Tabita, F. R., Hanson, T. E., Li, H., Satagopan, S., Singh, J., and Chan, S. Function, structure, and evolution of the RubisCO-like proteins and their RubisCO homologs. Microbiol. Mol. Biol. Reviews 71 (2007) 576-599. 205. John, D. E., Wang, Z.A., Liu, X., Byrne, R. H., Corredor, J. E., Lopez, J. M., Cabrera, A. Bronk, D. A., Tabita. F. R., and Paul, J. H. Phytoplankton carbon fixation gene (RubisCO) transcripts and air-sea CO2 flux in the Misssisssippi River plume. Int. Soc. Mic. Ecol. Journal 1 (2007) 517-531. 206. Tabita, F. R., Satagopan, S., Hanson, T. E, Kreel N. E, and Scott, S. S. Distinct form I, II, III, and IV Rubisco proteins from the three kingdoms of life provide clues about Rubisco evolution and structure/function relationships. J. Exp. Bot. 59 (2008) 1515-1524. 207. Tabita, F. R., Hanson, T. E., Satagopan, S., Witte, B. H., and Kreel N. E. The evolution, structure, and function of RubisCO and its homolog the RubisCO-like protein. Phil. Trans. Royal Soc. SerB 363 (2008) 563-576. 208. Romagnoli, S., and Tabita, F. R. Carbon dioxide metabolism and its regulation in nonsulfur purple photosynthetic bacteria. In, Hunter C.N., Daldal F., Thurnauer M.C., and Beatty J. T. (2008) The Purple Phototrophic Bacteria. Advances in Photosynthesis and Respiration, Vol. 28, Springer, Dordrecht, The Netherlands, pp. 563-576. 209. Imker, H. J., Singh, J., Warlick, B. P., Tabita, F. R. and Gerlt, J. A. Mechanistic diversity in the RuBisCO superfamily: A novel isomerization reaction catalyzed by the RuBisCO-like protein from Rhodospirillum rubrum. Biochemistry (2008) 11171-11173. 210. Dangel, A. W., and Tabita, F. R. Protein-protein interactions between CbbR and Reg(PrrA): transcriptional regulatorsof the cbb operons of Rhodobacter sphaeroides. Mol. Microbiol. (in press).

Current Support

1. NIH 5-RO1 GM077625-02 F. R. Tabita, PI at OSU for a joint UCLA-OSU project March 1, 2006-February 2009 5% effort

3 “Engineering large scale pathways into Escherichia coli.” Dr. James C. Liao from UCLA is the PI and this grant was awarded via the Interagency Metabolic Engineering Initiative (with FRT as CoPI) The long-term goal is to engineer new pathways into E. coli using genes from Rhodopseudomonas palustris

2. DOE DE-FG02-07ER64489 “Bioengineering and coordination of regulatory networks and intracellular complexes to maximize hydrogen production by phototrophic microorgansims”; F. R. Tabita, PI; Sept. 15, 2007 – Sept. 2010 The long-term goal is to elucidate the molecular control of interacting systems that impinge on bio-hydrogen production in nonsulfur purple bacteria

3. DOE DE-FG02-08ER15976 “Production of biofuels and value-added products via solar and chemical energy driven bioconversions of CO2 fixing microbes” F. R. Tabita, PI; Sept. 1, 2008 – August 31, 2011 The long-term goal of this project is to study the molecular control of CO2 fixation genes that affect the potential to convert CO2 to value-added chemicals

4. U. S. Air Force “Optimization of biofuel production from transgenic microalgae” R. Sayre, Danforth Foundation, PI; F. R. Tabita, CoPI; Sept. 1, 2008 to Aug. 31, 2011 The long term goal of this research is to maximize various biofuel production in algae after manipulation of molecular control circuits and key enzymes.

5. OARDC “Summer workshop in functional genomics” F. R. Tabita, PI; E. Grotewold, CoPI; Jan. 1, 2008 – Dec. 31, 2009. The goal is to provide partial support to operate a functional genomics training program for Ohio students in the area of plant molecular biology and microbiology

4 Biographical Sketch

Esther Klazina Maria van der Knaap Department of Horticulture and Crop Science, The Ohio State University/OARDC, Wooster, OH 44691; Tel: 330-263-3822; FAX: 330-263-3887; email: [email protected]; web: http://www.oardc.ohio-state.edu/vanderknaap

PROFESSIONAL PREPARATION Wageningen University, Wageningen, the Netherlands BS/MS 1990 Plant Pathology Michigan State University, East Lansing MI, USA Ph.D 1998 Genetics Cornell University, Ithaca NY, USA PD 1998-2001

PROFESSIONAL EXPERIENCE 2006-present Assistant professor, Department of Horticulture and Crop Science, OARDC/The Ohio State University, Wooster OH, USA 2001-2006 Research Scientist and Adjunct Assistant Professor, Department of Horticulture and Crop Science, OARDC/The Ohio State University, Wooster OH, USA. 1990-1992 Visiting researcher, Department of Nematology, University of California, Riverside CA, USA.

PUBLICATIONS most closely related to the project: Xiao, H., N. Jiang, E.K. Schaffner, E.J. Stockinger, E. van der Knaap. 2008. A retrotransposon-mediated gene duplication underlies morphological variation in tomato fruit. Science 319: 1527-1530. Featured on the cover of the journal Gonzalo, M.J. and E. van der Knaap 2008 A comparative analysis into the genetic bases of tomato varieties exhibiting elongated fruit shape. Theor Appl Genet 116: 647-656 Brewer, M.T., J.B. Moyseenko, A.J. Monforte, E. van der Knaap 2007. Morphological Variation in Tomato: A Comprehensive Study of Quantitative Trait Loci Controlling Fruit Shape and Development. J Exp Bot 58: 1339-1349 Van Deynze, A., K. Stoffel, C.R. Buell, A. Kozik, J. Liu, E. van der Knaap, D.M. Francis. 2007 Diversity in conserved genes in tomato. BMC Genomics 8:465. Brewer, M.T., L. Lang, K. Fujimura, N. Dujmovic, S. Gray, E. van der Knaap 2006. Development of a controlled vocabulary and software application to analyze fruit shape variation in tomato and other plant species. Plant Physiol 141: 15-25. Featured on the cover of the journal

Other significant publications: Olmstead, J.W., A.M. Sebolt, A. Cabrera, S.S. Sooriyapathirana, S. Hammar, G. Iriarte, D. Wang, C.Y. Chen, E. van der Knaap, A.F. Iezzoni. 2008. Construction of an intra-specific sweet cherry (Prunus avium L.) genetic linkage map and synteny analysis with the Prunus reference map. Tree Genet Genomics 4: 897-910. Darrigues, A., J. Hall, N. Dujmovic, S. Gray, E. van der Knaap, D.M. Francis. 2008. Tomato Analyzer – Color Test: a new tool for efficient digital phenotyping. J Am Soc Hort Sci 133: 579-586. Paran, I. and E. van der Knaap. 2007 Genetic and molecular regulation of fruit and plant domestication traits in tomato and pepper. J Exp Bot 58: 3841-3852 Tian, M., J. Win, J. Song, R. van der Hoorn, E. van der Knaap and S. Kamoun 2007. A Phytophthora infestans cystatin-like protein targets a novel tomato papain-like apoplastic protease. Plant Physiol 143: 364-377. Welty, N., C. Radovich, T. Meulia, E. van der Knaap 2007. Inflorescence development in two tomato species. Can J Bot 85: 111-118 SYNERGISTIC ACTIVITIES • Chair of the Council, Plant Molecular Biology and Biotechnology Program. 2006-2008. • Organizer, Wooster Area Molecular Biology Association weekly campus-wide seminar series. 2003-2006. • Hosted 13 summer undergraduate laboratory interns. Hosted Independent Studies projects for Sarah McNulty (ATI) and Erin Schaffner (CoWooster). • Computational undergraduate research projects with Dr. Simon Gray and students Nancy Dujmovic, Ben Strecker, Rick Drushal, Ellen Wagner and David Sullivan (CoWooster). This project resulted in several papers with undergraduate authors. • Database construction project with undergraduates Dustin Welty, Traian Andrei, Jason Berry under supervision of Dr. Denise Byrnes (CoWooster).

COLLABORATORS AND COAUTHORS IN THE LAST 4 YEARS Alarcon, A (IRTA, Barcelona Spain); Alvarez, JM (IRTA, Barcelona Spain); Arus, Pere (IRTA, Barcelona Spain); Bai, Xiaodong (Ohio State); Bedinger, Pat (Colorado State); Brewer, Marin (Ohio State); Buell, Robin (Mich State); Cabrera, Antonio (Ohio State); Chen, C. (Mich State); Chetelat, Roger (UC-Davis); Darrigues, Audrey (Ohio State); Drushal, Rick (College of Wooster); Dujmovic, Nancy (College of Wooster); Eaton, Christina (unknown); Eduardo, I (IRTA, Barcelona Spain); Fernández- Trujillo, JP (IRTA, Barcelona Spain); Francis, David (Ohio State); Fujimura, Kikuo (Ohio State); Gonzalo, Maria Jose (Zaragoza, Spain); Goodner, Brad (Hiram College); Gray, Simon (College of Wooster); Hall, Jack (Ohio State); Hammar, Sue (Mich State); Hogenhout, Saskia (John Innes, UK); Iezzoni, Amy (Michigan State); Iriarte, Gloria (Ohio State); Jackson, Scott (Purdue); Jiang, Ning (Michigan State); Kabelka, Eileen (U-Florida); Kamoun, Sophien (Sainsbury Labs, UK); Kozik, Alex (UC Davis); Labate, Joanne (USDA-ARS); Lang, Lixin (Ohio State); Liu, Jia (TIGR); Loescher, Wayne (Michigan State); Martinez, JA (IRTA, Barcelona Spain); McClure, Bruce (Univ of Missouri); Meulia, Tea (Ohio State); Monforte, Antonio (IRTA, Barcelona Spain); Moyseenko, Jenny (Ohio State); Obando, J (IRTA, Barcelona Spain); Olmstead, Jim (Wash State); Paran, Ilan (Volcani Center, Israel); Radovich, Cheryll (Ohio State); Reed, Jason (U of North Carolina); Rose, Joss (Cornell); Sanyal, Abhijit (Purdue); Schaffner, Erin (Ohio State); Sebolt, Audrey (Mich State); Song, Jing (Ohio State); Sooriyapathirana, Suneth (Mich State); Stack, Steve (Colorado State); Stockinger, Eric (Ohio State); Stoffel, Kevin (UC Davis); Strecker, Ben (College of Wooster); Tanksley, Steven (Cornell); Tian, Maoying (Michigan State); van der Hoorn, Renier (Max Planck, Cologne, Germany); VanDeynze, Allen (UC-Davis); Wang, Dechun (Michigan State); Welty, Nic (Mich State); Win, Joe (Ohio State); Yang, Wencai (Ohio State/China Agr University).

ADVISORS Post doctoral: Dr. Steven D. Tanksley, Cornell University Ph.D.: Dr. Hans Kende, Michigan State University, deceased

CURRENT ADVISEES Post doctoral researchers: Han Xiao, Gustavo Rodriguez, Zejun Huang Ph.D.: Na Zhang, Shan Wu MS.: Antonio Cabrera Visiting scientist: Reynaldo Nunez

PAST ADVISEES Post doctoral researcher: Maria Jose Gonzalo, Zaragoza, Spain MS: Nic Welty, Michigan State Guo-Liang Wang, PhD Professor, Department of Plant Pathology The Ohio State University, Columbus, OH, Phone (614) 292 9280; Fax (614) 292 4455 Email: [email protected], lab website: http://plantpath.osu.edu/~wang/ Education: Ph.D. 1992, Plant Genetics and Breeding, University of the Philippines at Los Banos and International Rice Research Institute (IRRI), Philippines. M.Sc. 1985, Plant Genetics, Fujian Agricultural University, Fuzhou, China. B.Sc. 1982, Plant Genetics, Hunan Agricultural University, Changsha, China Research/Professional Experience: Professor, Department of Plant Pathology, Ohio State University, Columbus, Ohio. 10/2008-present. Associate Professor, Dept of Plant Pathology, Ohio State University, Ohio. 5/2004-9/2008. Adjunct Professor, Hunan Agricultural University, Hunan, China, 3/2004-present. Adjunct Professor, Institute of Genetics, Chinese Academy of Sciences, 1996- present Assistant Professor, Department of Plant Pathology, Ohio State University, Columbus, Ohio. 10/1999-5/2004. Senior Scientist and Principal Investigator, Institute of Molecular Agrobiology, The National University of Singapore, 8/1996-9/99. Postdoctoral Research Associate, University of California at Davis, 8/1993-8/96 Postdoctoral Research Associate, Texas A&M University, 6/1992-8/1993.

Awards, Honors and Services th • Organizer, the 4 International Conference of Rice Blast, Oct. 10-14, 2007, Changsha, China. • Editorial Board of Journal of Plant Biology, July 2007. • Research Award of Merit from Gamma Sigma Delta, the Honor Society of Agriculture, OSU, 4/2007 • Member, Editorial Board of Plant Science, 2/2007-present • The Syngenta Award of the American Phytopathology Society, 8/2006 • Panel member, USDA-NRI Functional Genomics, 10/2005 • OARDC Distinguished Junior Faculty Research Award, 5/2005 • FuRong Scholar, Hunan Agricultural University, Hunan, China, 3/2004-present • Panel member: OARDC Research Committee, 9/2004-present • Panel member: USDA-NRI Genetic Mechanism, 3/2004 • Outstanding Overseas Young Scientist Award, Natural Science Foundation of China (6/2001) • DuPont Young Professor Award (8/2000) Society Memberships: American Association for the Advancement of Science (AAAS), American Society for Plant Biologists (ASPB), American Phytopathological Society (APS) Funding Agency: NSF-Plant Genome Research, USDA-NRI, Ohio Agricultural Research and Development Center (OARDC) and USAID. Research Interests: Plant Functional Genomics and Disease Resistance

Publications in the last three years (total 25) 1. Li-Rong Zeng, Chan Ho Park, R.C. Venu, Julian Gough and Guo-Liang Wang. 2008.Classification, expression pattern, and E3 ligase activity assay of rice U-Box-containing proteins. Molecular Plant, in press. 2. Vega-SÃ¡nchez ME, Zeng L, Chen S, Leung H, Wang GL. 2008. SPIN1, a K Homology Domain Protein Negatively Regulated and Ubiquitinated by the E3 Ubiquitin Ligase SPL11, Is Involved in Flowering Time Control in Rice. The Plant Cell, 1456-1469. 3. Wu C, Bordeos A, Madamba MR, Baraoidan M, Ramos M, Wang GL, Leach JE, Leung H. 2008. Rice lesion mimic mutants with enhanced resistance to diseases. Mol Genet Genomics. 279(6):605-19. 4. Shujie Dong, Lane P. Tredway, H. David Shew, Guo-Liang Wang, Elumalai Sivamani, Rongda Qu. 2007. Resistance of transgenic tall fescue to two major fungal diseases. Plant Science, 173:501-509.

1 5. Rose Palumbo, Wai-Foong Hong, Jinguo Hu, Richard Craig, James Locke, Charles Krause, David Tay and Guo-Liang Wang. 2007. Target Region Amplification Polymorphism (TRAP) as a Tool for Detecting Genetic Variation in the Genus Pelargonium. HortScience 42:1118- 1123. 6. Venu RC, Jia Y, Gowda M, Jia MH, Jantasuriyarat C, Stahlberg E, Li H, Rhineheart A, Boddhireddy P, Singh P, Rutger N, Kudrna D, Wing R, Nelson JC, Guo-Liang Wang. 2007. RL-SAGE and microarray analysis of the rice transcriptome after Rhizoctonia solani infection. Mol Genet Genomics. 278: 421-431. 7. Miguel E. Vega-Sánchez, Malali Gowda and Guo-Liang Wang. 2007. Tag-based approaches for deep transcriptome analysis in plants. Plant Sciences, 173:371-380. 8. Malali Gowda, Huameng Li, Guo-Liang Wang. 2007. Robust analysis of 5’-transcript ends (5’-RATE): a high-throughput protocol for characterization of sequence diversity of transcription start sites. Nature Protocols. 2(7):1622-32. 9. Malali Gowda, R-C. Venu, Huameng Li, Chatchawan Jantasuriyarat, Songbiao Chen, Maria Bellizzi, Vishal Pampanwar, HyeRan Kim, Ralph A. Dean, Eric Stahlberg, Rod Wing, Cari Soderlund, Guo-Liang Wang. 2007. Magnaporthe grisea Infection Triggers RNA Variation and Antisense Transcript Expression in Rice, Plant Physiology, 144(1):524-33. 10. Kan Nobuta, Venu Reddyvari-Channarayappa, Cheng Lu, André Belo, Kalyan Vemaraju, Pam Green, Guo-liang Wang, and Blake C. Meyers. 2007. An Expression Atlas of Rice mRNA and Small RNA", Nature Biotechnology, 25:473-477. 11. Gowda M, RC Venu, Mohan B Raghupathy, Kan Nobuta, Huameng Li, Eric Stahlberg, Rod Wing, Sean Coughlan, Christian D Haudenschild, Ralph Dean, Baek Hie Nahm, Blake C Meyers and GL Wang. 2006. Deep and comparative analysis of the mycelium and appressorium transcriptomes of Magnaporthe grisea using MPSS, RL-SAGE, and oligoarray methods. BMC Genomics, 8;7:310. 12. Liangying Dai, Xionglun Liu, Yinghui Xiao, GL Wang. 2007 Recent advances in cloning and characterization of disease resistance genes in rice. Journal of Integrative Plant Biology. 49 (1): 112-119. 13. Gowda M, Reddyvarichannarayappa Venu, Jia Y, Stahlberg E, Pampanwar V, Soderlund C, GL Wang (2007). Use of RL-SAGE Analysis to Identify Novel Fungal and Plant Genes Involved in Host-Pathogen Interactions. Methods Mol Biol. 2006;354:131-44. Synergistic Activities: 1. Curriculum development: Agricultural Genomics PP703 2. Co-PI for the rice blast project, NSF funded 06-09 3. Co-PI for the rice MPSS transcriptome analysis, NSF funded 03-07 4. Co-PI for the rice epigenomic analysis, NSF funded 07-11 5. Member of interdisciplinary graduate training programs: Plant Molecular Biology and Biotechnology (PMBB) at OSU Collaborators: Pamela Ronald, UC Davis; Hajime Sakai, DuPont; Hei Leung, The International Rice Research Institute, Philippines; Jan Leach, Kansas State University, Ralph A. Dean, North Carolina State University, Rod Wing, University of Arizona, Cari Soderlund, University of Arizona, Blake Meyers, University of Delaware, Steve Jacobsen, UCLA, Liangying Dai, Hunan Agricultural University, Advisees in Past Five Years Guodong Lu, Professor, Fujian Agricultural and Forest University, China, Shaohong Qu, Postdoc fellow, UC Davis, Lirong Zeng, Postdoc, Cornell University, Malali Gowda, Research Scientist, NCSU, Bo Zhou, Associate Professor, CAS, Shanghai, China, Chat Jantasuriyarat. Assistant Professor, Kasetsart University, Thailand Current Advisees Postdocs: Songbiao Chen, Honggui La, Bo Ding, Xinli Sun, IP Ahn, Maofeng Chai, Bo Zhou Gradudate students: Miguel Vega-Sanchez, Huameng Li, Chan Ho Park, Pattavipha Songkumarn, Gautaum Shirsekar Visiting Scientists: Jianli Liu, Sheshu Magati, Zhongyou Pei