DOCSLIB.ORG

Anolis Carolinensis Expression of Igm, Igd, and Igy in a Reptile

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Anolis Carolinensis Expression of Igm, Igd, and Igy in a Reptile The Journal of Immunology Expression of IgM, IgD, and IgY in a Reptile, Anolis carolinensis1 Zhiguo Wei,2* Qian Wu,2* Liming Ren,* Xiaoxiang Hu,* Ying Guo,* Gregory W. Warr,3† Lennart Hammarström,‡ Ning Li,4* and Yaofeng Zhao4* The reptiles are the last major group of jawed vertebrates in which the organization of the IGH locus and its encoded Ig H chain isotypes have not been well characterized. In this study, we show that the green anole lizard (Anolis carolinensis) expresses three Ig H chain isotypes (IgM, IgD, and IgY) but no IgA. The presence of the ␦ gene in the lizard demonstrates an evolutionary continuity of IgD from ␦ fishes to mammals. Although the germline gene contains 11 CH exons, only the first 4 are used in the expressed IgD membrane-bound ␮ form. The chain lacks the cysteine in CH1 that forms a disulfide bond between H and L chains, suggesting that (as in IgM of some amphibians) the H and L polypeptide chains are not covalently associated. Although conventional IgM transcripts (four CH domains) encoding both secreted and membrane-bound forms were detected, alternatively spliced transcripts encoding a short membrane-bound form were also observed and shown to lack the first two CH domains (VDJ-CH3-CH4-transmembrane region). Similar to duck IgY, ␷ ⌬ lizard IgY H chain ( ) transcripts encoding both full-length and truncated (IgY Fc) forms (with two CH domains) were observed. The absence of an IgA-encoding gene in the lizard IGH locus suggests a complex evolutionary history for IgA in the saurian lineage leading to modern birds, lizards, and their relatives. The Journal of Immunology, 2009, 183: 3858–3864. he Igs are uniquely characteristic of the adaptive immune which are encoded by the ␮, ␦, ␥, ␧, and ␣ genes, respectively (1, systems of all jawed vertebrates, being found in jawed fish, 2). The evolutionary history of these genes is of interest from many T amphibia, reptiles, birds, and mammals (1, 2). A typical Ig perspectives, two of which have received particular attention. The molecule consists of two H and two L chains, with the H and L chains first is the question of how the genes encoding the H chains arose, being encoded in separate IGH5 and IGL loci (2). The H and L one from the other, and how their subsequent evolutionary diver- polypeptides are typically covalently linked by disulfide bonds gence permitted the acquisition of novel Ab functions including formed by positionally conserved cysteine residues; however, nonco- transport and secretion into external body fluids such as mucus and valent interactions alone are sufficient to hold L and H chains together, milk, or the mediation of allergic reactions. In summary, first, IgM as observed for human IgA2m (1) and Igs of Rana catesbeiana (3, 4). is the only class of Ab that is expressed in all species of jawed The Ig classes expressed in mammals are IgM, IgD, IgG, IgE, vertebrates (1, 2, 5). Second, IgD is a class of Ab that is probably and IgA. This classification is based on their H chain isotypes, as ancient as IgM, existing in elasmobranchs as a class that was originally named IgW but is now recognized as a homolog of IgD (6). IgD is an enigmatic Ab the structure of which is highly vari- *State Key Laboratory of Agrobiotechnology, College of Biological Sciences, able (which, in some cases, is due to duplication or loss of CH † China Agricultural University, Beijing, Peoples Republic of China; Medical Uni- exons; Ref. 7), the function of which is a matter of debate (8), and versity of South Carolina, Marine Biomedicine and Environmental Sciences Cen- ter, Charleston, SC 29425; and ‡Division of Clinical Immunology, Department of which has clearly been lost from certain species. In bony fish and Laboratory Medicine, Karolinska University Hospital Huddinge, Stockholm, pigs, IgD can be expressed as chimeric H chains with inclusion of Sweden the IgM CH1 domain (9, 10). Although IgD/W has been described Received for publication September 30, 2008. Accepted for publication July 13, 2009. in fish (cartilaginous and bony), amphibians, and numerous mam- The costs of publication of this article were defrayed in part by the payment of page mals (6, 9, 11, 12), it is absent in birds and rabbits (13, 14). Third, charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. the major class of low-m.w. Ab in the amphibia, reptiles, and birds 1 This work was supported by National Science Fund for Distinguished Young Schol- is IgY, which is thought to have been derived from IgM by a gene ars Grant 30725029, Program for New Century Excellent Talents in University of duplication event (5). Fourth, IgY played a central role in Ab evo- China, National Key Basic Research Program Grant 2006CB102100, and National Natural Science Foundation of China Grant 30671497. lution in the tetrapod lineage, giving rise to IgA (through an ap- parent recombination event with IgM; Ref. 15) and to IgG and IgE This material is based in part on work supported by the National Science Foundation. Any opinion, finding, and conclusions or recommendations expressed in this material through a gene duplication event followed by differentiation of are those of the author and do not necessarily reflect the views of the National Science structure and function (16). Overlaid on this broad scheme are Foundation. other important events in the evolutionary history of Abs where 2 Z.W. and Q.W. contributed equally to this work. certain lineages developed unique classes of Abs, such as the Ig- 3 Current address: Division of Molecular and Cellular Biosciences, National Science NAR in elasmobranchs (17), IgT/Z in some species of bony fish Foundation, 4201 Wilson Boulevard, Arlington, VA 22230. (18, 19), and IgX and IgF in amphibia (12, 20). 4 Address correspondence and reprint requests to Dr. Yaofeng Zhao, State Key Lab- oratory of Agrobiotechnology, College of Biological Sciences, China Agricultural Uni- A second important perspective on the evolution of the Ab versity, Beijing 100193, Peoples Republic of China. E-mail address: yaofengzhao@ genes is the structure of the IGH locus. Interest in this question was cau.edu.cn, or Dr. Ning Li, State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, Peoples Repub- stimulated by observations that in cartilaginous fish (phylogeneti- lic of China. E-mail address: [email protected] cally the most primitive jawed vertebrates), the IGH loci show a 5 Abbreviations used in this paper: IGH, Ig H chain gene; IGL, Ig L chain gene; unique structure. Whereas in the mammals the genes present in the BLAST, basic local alignment search tool; NCBI, National Center for Biotechnology IGH locus are arranged in a (V ) -(D ) -(J ) -(C ) order, the Information; IgSF, Ig superfamily; TM, transmembrane region; sIg, secretory Ig. H n H n H n H n IGH loci of cartilaginous fish show a multicluster organization, Copyright © 2009 by The American Association of Immunologists, Inc. 0022-1767/09/$2.00 where multiple clusters, each consisting of (V-DH-DH-JH-CH), www.jimmunol.org/cgi/doi/10.4049/jimmunol.0803251 The Journal of Immunology 3859 are arranged in tandem (21–23). These alternative organizations and IgY cDNA. All the resulting PCR products were cloned into a T vector (translocon or multicluster) present different challenges for the or- (pMD19; Takara) and sequenced. The sequences have been deposited in derly rearrangement and clonal expression by B lymphocytes of the NCBI GenBank under the following accession numbers: EF683585 and EF690357–EF690361 (http://www.ncbi.nlm.nih.gov/sites/entrez). the Ab H chains (24). Although the IGH loci have been extensively investigated in Southern blotting fish, amphibia, birds, and mammals, our current knowledge of the The above amplified ␮ (membrane-bound form), ␦ (membrane-bound Ig genes in reptiles remains limited. Reptiles are known, on the form), and ␷ (secreted form) cDNAs were used as probes, which were all basis of studies at the protein level, to express two Ig classes, IgM labeled using a PCR digoxygenin probe synthesis kit (Roche). The hybrid- and IgY (25, 26), and an IgM encoding gene has been cloned in ization and detection were conducted by following the manufacturer’s digoxygenin hybridization instruction. turtles (27). Two types of IgY (7S, 5.7S) that differ in the lengths of their H chain have been described at the biochemical level in Tissue expression of the lizard IGH genes turtles (28, 29), but it is not known whether the genetic basis of this Tissue expression of the membrane-bound and secreted IgM were detected phenomenon is the same as that underlying the expression of two by PCR using the primers IgMCH3s (5Ј-CATCAGTGGCATCCCTCA isoforms of IgY in the duck (30). A gene encoding an IgA-like CA-3Ј) and IgMTMas3 (5Ј-ATCCCATCTAGGTAAGCCGT-3Ј) or lizard isotype was recently cloned in the leopard gecko (15), suggesting that sIgMas2. Full-length IgY expression was detected using primer IgY de- tections (5Ј-TTTCCTGATCCGGTCACAGTGC-3Ј) and IgY detections the repertoire of Ig classes in reptiles may be similar to that of birds. (5Ј-AGCCCTTCTTCACTTTCCAGAT-3Ј), whereas IgY (⌬Fc) expres- The IgD-encoding gene, despite its absence in birds, was also recently sion was detected using primer IgY detections and IgYDFc detections (5Ј- reported in the gecko (31). A paucity of knowledge of the structure of CAACAATTAGATGCCCATAC-3Ј). Expression of IgD was detected by the reptilian IGH locus and of its encoded Ig H chain isotypes has a nested PCR using primers JHs1 and IgDTMas1, JHs2 and IgDTMas2. been a barrier to a more complete understanding of the evolutionary Analysis of the expressed Ig isotypes in the small intestine history of Igs in vertebrates.
Load more

Recommended publications
  • Anolis Aeneus (Bronze Anole)
    UWI The Online Guide to the Animals of Trinidad and Tobago Behaviour Anolis aeneus (Bronze Anole) Family: Polychrotidae (Anoles) Order: Squamata (Lizards and Snakes) Class: Reptilia (Reptiles) Fig. 1. Bronze anole, Anolis aeneus. [http://www.trinidad-tobagoherps.org/Anolisaeneus.htm, downloaded 20 October 2012] TRAITS. Anolis aeneus can be distinguished by the blue-green ring around its eyes (Murphy 2011). The species is a medium sized anole, the length of males from the tip of the nose to the anus is 77 mm and females 55 mm (John et al. 2012). They have many lamellae (flaps) on their subdigital toepads. The dewlap (throat fan) extends from underneath their necks and has a pale gray, green or white colour, yellow or orange spots may also be present at the front edge of the dewlap (John et al. 2012). Colour: The dorsal side of the males may be grey, greyish brown, brown or a dull green, a bronze sheen is at times present, light or dark spots may be present in a crosswise pattern. The underside has a dull grey colour. The females may be grey or brown the mid-dorsal region can include a dark single stripe or a transverse stripe, juveniles are dark grey or brown (John et al. 2012). ECOLOGY. This species is endemic to Grenada and has been introduced to Trinidad and Tobago (Wikipedia 2012). It is an arboreal species and can therefore be found mostly on the trunk and branches of shaded trees, it is also populated in urban areas and can be observed on walls, railings and fences (Murphy 2011) it feeds on live insects and invertebrates such as crickets, roaches and spiders.
    [Show full text]
  • Anolis Planiceps (Leaf Anole)
    UWI The Online Guide to the Animals of Trinidad and Tobago Diversity Anolis planiceps (Leaf Anole) Family: Polychrotidae (Anoles and Tree Lizards) Order: Squamata (Lizards and Snakes) Class: Reptilia (Reptiles) Fig. 1. Leaf anole, Anolis planiceps. [http://www.trinidad-tobagoherps.org/Images/planiceps.jpg, downloaded 24 October 2016] TRAITS. Formerly known as Anolis chrysolepis or Norops chrysolepis, the leaf anole measures up to 76mm from snout to vent according (D'Angiolella et al., 2011). The pads of their feet are specialised to help them rest on leaves and trunks (Fig. 1). They have a spotted red patch of skin below theirs jaws, which is extendable, called the dewlap (Fig. 2). The region along the lizard's spine has larger scales than the adjacent areas with those located in the mid-dorsal area being the largest. Along their heads are two prominent ridges as well as ridged (keeled) scales located above the eyes (Fig. 3). The dorsal scales of the leaf anole are several shades of brown while the ventral scales are a pale cream colour; patterns vary greatly within populations (Fig. 4) (Vanzolini and Williams, 1970). Male anoles have longer tails and the females have wider bodies and smaller dewlaps than males (Vitt and Zani, 2011). DISTRIBUTION. Leaf anoles may be found in a relatively wide range from east Venezuela to Guyana, Suriname, Columbia, Trinidad and Brazil (Fig. 5). They are found throughout the island of Trinidad primarily in terrestrial, highly forested areas (D'Angiolella et al, 2011). UWI The Online Guide to the Animals of Trinidad and Tobago Diversity HABITAT AND ECOLOGY.
    [Show full text]
  • And Resurrection of Anolis (Diaphoranolis) Brooksi 1Steven Poe and 2Mason J
    Ofcial journal website: Amphibian & Reptile Conservation amphibian-reptile-conservation.org 11(2) [General Section]: 1–16 (e141). urn:lsid:zoobank.org:pub:31FA8B4B-718B-4440-AE19-9E1AC95524BD Description of two new species similar to Anolis insignis (Squamata: Iguanidae) and resurrection of Anolis (Diaphoranolis) brooksi 1Steven Poe and 2Mason J. Ryan 1,3Department of Biology and Museum of Southwestern Biology, University of New Mexico, Albuquerque, New Mexico 87131, USA 2Arizona Game and Fish Department, 5000 W. Carefree Highway, Phoenix, AZ 85086, USA Abstract.—The spectacular giant anole lizard Anolis insignis is widely distributed but infrequently collected outside of northern Costa Rica. We recently collected several individuals similar to Anolis insignis from localities in Panama and southern Costa Rica. These populations difer from type locality A. insignis in male dewlap color and morphology. We associate one set of these populations with Anolis (Diaphoranolis) brooksi Barbour from Darién, Panama, and describe two additional populations as new species. Keywords. Central America, Costa Rica, lizard, Panama, Reptilia, taxonomy Citation: Poe S and Ryan MJ. 2017. Description of two new species similar to Anolis insignis (Squamata: Iguanidae) and resurrection of Anolis (Diaphoranolis) brooksi. Amphibian & Reptile Conservation 11(2) [General Section]: 1–16 (e141). Copyright: © 2017 Poe and Ryan. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial- NoDerivatives 4.0 International License, which permits unrestricted use for non-commercial and education purposes only, in any medium, provided the original author and the ofcial and authorized publication sources are recognized and properly credited. The ofcial and authorized publication credit sources, which will be duly enforced, are as follows: ofcial journal title Amphibian & Reptile Conservation; ofcial journal website <amphibian- reptile-conservation.org>.
    [Show full text]
  • Testing Sustainable Forestry Methods in Puerto Rico
    Herpetology Notes, volume 8: 141-148 (2015) (published online on 10 April 2015) Testing sustainable forestry methods in Puerto Rico: Does the presence of the introduced timber tree Blue Mahoe, Talipariti elatum, affect the abundance of Anolis gundlachi? Norman Greenhawk Abstract. The island of Puerto Rico has one of the highest rates of regrowth of secondary forests largely due to abandonment of previously agricultural land. The study was aimed at determining the impact of the presence of Talipariti elatum, a timber species planted for forest enrichment, on the abundance of anoles at Las Casas de la Selva, a sustainable forestry project located in Patillas, Puerto Rico. The trees planted around 25 years ago are fast-growing and now dominate canopies where they were planted. Two areas, a control area of second-growth forest without T. elatum and an area within the T. elatum plantation, were surveyed over an 18 month period. The null hypothesis that anole abundance within the study areas is independent of the presence of T. elatum could not be rejected. The findings of this study may have implications when designing forest management practices where maintaining biodiversity is a goal. Keywords. Anolis gundlachi, Anolis stratulus, Puerto Rican herpetofauna, introduced species, forestry Introduction The secondary growth forest represents a significant resource base for the people of Puerto Rico, and, if At the time of Spanish colonization in 1508, nearly managed properly, an increase in suitable habitat one hundred percent of Puerto Rico was covered in for forest-dwelling herpetofauna. Depending on the forest (Wadsworth, 1950). As a result of forest clearing management methods used, human-altered agro- for agricultural and pastureland, ship building, and fuel forestry plantations have potential conservation wood, approximately one percent of the land surface value (Wunderle, 1999).
    [Show full text]
  • (Squamata: Iguania) from the Central Andes of Colombia
    HERPETOLOGICAL JOURNAL 20: 231–236, 2010 A new species of Anolis of the aequatorialis group (Squamata: Iguania) from the central Andes of Colombia Julián Andrés Velasco1, Paul David A. Gutiérrez-Cárdenas2 & Andrés Quintero-Angel1 1Grupo de Ecología Animal, Departamento de Biología, Universidad del Valle, Cali, Colombia 2Grupo Herpetológico de Antioquia (sede Caldas), Departamento de Ciencias Biológicas, Universidad de Caldas, Manizales, Colombia We describe a new species of the Anolis aequatorialis group from the central Andes of Colombia. The new species, Anolis anoriensis, is similar to A. eulaemus Boulenger, which occurs in both the western and central Andes, and was positioned in the eulaemus subgroup of the aequatorialis group. Anolis anoriensis differs from A. eulaemus in having smaller interparietal scales and a green body coloration with a darker anterior part of the dewlap. We also for the first time describe the coloration of Anolis eulaemus, which is almost exclusively brown with a diffused light brown dewlap. Key words: Anolis anoriensis sp. nov., taxonomy, morphology INTRODUCTION distributions in Colombia and Ecuador: A. antioquiae, A. eulaemus, A. fitchi, A. gemmosus, A. maculigula, A. meg- he Andes of Colombia are a recognized global biodi- alopithecus and A. ventrimaculatus. Tversity hotspot (Myers et al., 2000). However, many In this paper we describe a new species of Anolis of taxonomic groups have been poorly sampled in this re- the eulaemus subgroup of alpha anoles (Etheridge, 1959), gion, despite a large number of species discoveries in the from the Department of Antioquia in the Cordillera Cen- last decade. Anolis lizards are one of these poorly studied tral of Colombia.
    [Show full text]
  • History of Squamate Lizard Dac
    History of Squamate Lizard Dactyloidae from the Eastern Caribbean, Origins of Anolis from Martinique, Zanndoli Matinik (Dactyloa roquet) Marcel Bourgade To cite this version: Marcel Bourgade. History of Squamate Lizard Dactyloidae from the Eastern Caribbean, Origins of Anolis from Martinique, Zanndoli Matinik (Dactyloa roquet). 2020. hal-02469738 HAL Id: hal-02469738 https://hal.archives-ouvertes.fr/hal-02469738 Submitted on 6 Feb 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Martinique, January 2020 History of Squamate Lizard Dactyloidae from the Eastern Caribbean Origins of Anolis from Martinique, Zanndoli Matinik (Dactyloa roquet) by Marcel BOURGADE 56 islet of Pointe Marin, 97227 Sainte-Anne, Martinique, Eastern Caribbean [email protected] 1 Summary – The Anolis of Martinique, Zanndoli (in Martinique), the species of reptile lizard Dactyloa roquet represents with the species of amphibian Hylode of Johnstonei, Eleutherodactylus johnstonei, the two species of herpetofauna endemic to the eastern Caribbean, the most widely widespread and present in large numbers throughout the territory of Martinique. The history of the Dactyloidae of the eastern Caribbean that we retrace is based on the most recent data publications, in terms of research in molecular systematics, crossed with the data of the geological history of this geographical region of the Eastern Caribbean.
    [Show full text]
  • Predation of White Anole (Anolis Laeviventris) by Blue-Crowned Motmot (Momotus Momota) in a Montane Forest Reserve in Veracruz, Mexico
    Herpetology Notes, volume 7: 721-722 (2014) (published online on 21 December 2014) Predation of White Anole (Anolis laeviventris) by Blue-crowned motmot (Momotus momota) in a montane forest reserve in Veracruz, Mexico Adriana Sandoval-Comte*, Alma Patricia Degante-González and Diego Santiago-Alarcon Anole lizards are common, widely distributed, and a dead lizard in its beak (Figure 1). The lizard was they are eaten by spiders, frogs, other lizards, snakes, identified as a female Anolis laeviventris (the dewlap birds, and mammals (Losos and Greene, 2009). Anolis was not well-developed as in males, but we were able laeviventris is distributed from Southeastern Mexico to observe the characteristic coloration of the species), to Central America (EOL, 2014). Here we report a a common reptile in this region that is characterized by predation event on an adult A. laeviventris by a Blue- having a white dewlap. Adults and juveniles are usually crowned motmot (Momotus momota). The White anole found foraging on leaves, which is the period when is a common arboreal lizard in the central region of Veracruz, Mexico. Its natural history is poorly known, however, and we have no information regarding its ecological interactions. The members of the genus Momotus (Aves: Momotidae) are large and sedentary birds presumably requiring less relative energy intake compared to smaller birds (Jones 2009). M. momota is found mostly in cloud montane forest (Orejuela, 1980) of Middle and South America (Greeney et al., 2006, Snow 2001, Stiles 2009, Skutch 1945). M. momota is omnivorous, feeding mainly on arthropods and fruits, its diet may also include frogs (Master 1999), snakes (Stiles and Skutch 1989), mammals (Delgado-V.
    [Show full text]
  • Reptile Diversity in an Amazing Tropical Environment: the West Indies - L
    TROPICAL BIOLOGY AND CONSERVATION MANAGEMENT - Vol. VIII - Reptile Diversity In An Amazing Tropical Environment: The West Indies - L. Rodriguez Schettino REPTILE DIVERSITY IN AN AMAZING TROPICAL ENVIRONMENT: THE WEST INDIES L. Rodriguez Schettino Department of Zoology, Institute of Ecology and Systematics, Cuba To the memory of Ernest E. Williams and Austin Stanley Rand Keywords: Reptiles, West Indies, geographic distribution, morphological and ecological diversity, ecomorphology, threatens, conservation, Cuba Contents 1. Introduction 2. Reptile diversity 2.1. Morphology 2.2.Habitat 3. West Indian reptiles 3.1. Greater Antilles 3.2. Lesser Antilles 3.3. Bahamas 3.4. Cuba (as a study case) 3.4.1. The Species 3.4.2. Geographic and Ecological Distribution 3.4.3. Ecomorphology 3.4.4. Threats and Conservation 4. Conclusions Acknowledgments Glossary Bibliography Biographical Sketch Summary The main features that differentiate “reptiles” from amphibians are their dry scaled tegument andUNESCO their shelled amniotic eggs. In– modern EOLSS studies, birds are classified under the higher category named “Reptilia”, but the term “reptiles” used here does not include birds. One can externally identify at least, three groups of reptiles: turtles, crocodiles, and lizards and snakes. However, all of these three groups are made up by many species that are differentSAMPLE in some morphological characters CHAPTERS like number of scales, color, size, presence or absence of limbs. Also, the habitat use is quite variable; there are reptiles living in almost all the habitats of the Earth, but the majority of the species are only found in the tropical regions of the world. The West Indies is a region of special interest because of its tropical climate, the high number of species living on the islands, the high level of endemism, the high population densities of many species, and the recognized adaptive radiation that has occurred there in some genera, such as Anolis, Sphaerodactylus, and Tropidophis.
    [Show full text]
  • Florida's Introduced Reptiles: Brown Anole (Anolis Sagrei)1
    WEC441 https://doi.org/10.32473/edis-uw486-2021 Florida’s Introduced Reptiles: Brown Anole (Anolis sagrei)1 Steve A. Johnson, Courtney Reyes, Brandon Dodge, and Natalie M. Claunch2 Introduction python. This large species of snake was imported for the exotic pet trade, and by way of escapes and/or purposeful Florida has the unfortunate distinction of being the global releases of pets, became established in the Everglades. epicenter for nonnative reptiles, due to the intentional or Scientific studies have shown invasive pythons have caused unintentional actions of people. The state’s mild climate, severe declines of native mammals in the Everglades (4, abundant rainfall, expansive areas modified by humans, 5) and have introduced parasites affecting Florida’s native numerous international ports of entry (sea and air), as well snakes (6, 7). Further, state and federal agencies have spent as a thriving exotic pet trade all contribute to reptile inva- millions of dollars to manage additional impacts of pythons sions. Historically, the introduction of reptiles into Florida and prevent them from expanding their range farther north was unintentional; reptiles were stowaways in shipments of in the Peninsula and south into the Florida Keys. cargo. The first documented reptile introduction to Florida was that of the brown anole (Anolis sagrei) in the late This publication summarizes general knowledge about 1800’s (1). This small lizard likely arrived accidentally in a the brown anole in Florida. It’s one in a series of similar shipment of cargo originating from Cuba. Since then, more publications showcasing a suite of commonly seen or than 150 additional nonnative reptile species have been unique introduced reptiles that are established in the state.
    [Show full text]
  • Invasive Reptiles and Amphibians of Florida!
    1 Invasive Reptiles and Amphibians of Florida! 2 Created by: Thompson Antony Lauren Diaz Sean McKnight Alana Palau JoAnna Platzer Illustrated by Lauren Diaz Invasive Ecology of Reptiles and Amphibians Dr. Steven Johnson & Dr. Christina Romagosa University of Florida 2014 ---------------------------------------------------------------------------------------------------------------------------- Welcome What is an invasive species? Where are they found in Florida? What do they look like? How can I help? You came to the right coloring book, my friend! These questions and so many more can be answered by grabbing some crayons and turning the page. Things you will need: Your favorite coloring utensils Thinking cap 3 What is an INVASIVE SPECIES ??? An invasive species is a plant or animal that is moved from its native area to another and causes economic or environmental harm to the new area. There are 6 main ways a plant or animal can move from its native home to a non-native area: 1. Biological control: moved by humans to help control other animals 2. Hitchhike: catches a free ride on cars, boats, or planes 3. Food: Some people eat frog legs or other animal parts 4. Plant trade: hiding in a plant is a sneaky way to travel 5. Pet Trade: people want animals they can’t find in their backyard 6. Intentional: Someone brought it for some specific reason For many invasive reptiles and amphibians in Florida, the animals came through the pet trade. 4 Brown Anole Anolis sagrei This is a Cuban Brown Anole! The brown anole is originally from Cuba, but came to Florida when it hitched a ride on some cargo.
    [Show full text]
  • It Is Time for a New Classification of Anoles (Squamata: Dactyloidae)
    Zootaxa 3477: 1–108 (2012) ISSN 1175-5326 (print edition) www.mapress.com/zootaxa/ ZOOTAXA Copyright © 2012 · Magnolia Press Monograph ISSN 1175-5334 (online edition) urn:lsid:zoobank.org:pub:32126D3A-04BC-4AAC-89C5-F407AE28021C ZOOTAXA 3477 It is time for a new classification of anoles (Squamata: Dactyloidae) KIRSTEN E. NICHOLSON1, BRIAN I. CROTHER2, CRAIG GUYER3 & JAY M. SAVAGE4 1Department of Biology, Central Michigan University, Mt. Pleasant, MI 48859, USA. E-mail: [email protected] 2Department of Biology, Southeastern Louisiana University, Hammond, LA 70402, USA. E-mail: [email protected] 3Department of Biological Sciences, Auburn University, Auburn, AL 36849, USA. E-mail: [email protected] 4Department of Biology, San Diego State University, San Diego, CA 92182, USA. E-mail: [email protected] Magnolia Press Auckland, New Zealand Accepted by S. Carranza: 17 May 2012; published: 11 Sept. 2012 KIRSTEN E. NICHOLSON, BRIAN I. CROTHER, CRAIG GUYER & JAY M. SAVAGE It is time for a new classification of anoles (Squamata: Dactyloidae) (Zootaxa 3477) 108 pp.; 30 cm. 11 Sept. 2012 ISBN 978-1-77557-010-3 (paperback) ISBN 978-1-77557-011-0 (Online edition) FIRST PUBLISHED IN 2012 BY Magnolia Press P.O. Box 41-383 Auckland 1346 New Zealand e-mail: [email protected] http://www.mapress.com/zootaxa/ © 2012 Magnolia Press All rights reserved. No part of this publication may be reproduced, stored, transmitted or disseminated, in any form, or by any means, without prior written permission from the publisher, to whom all requests to reproduce copyright material should be directed in writing. This authorization does not extend to any other kind of copying, by any means, in any form, and for any purpose other than private research use.
    [Show full text]
  • Reptiles and Mammals Have Differentially Retained Long Conserved Noncoding Sequences from the Amniote Ancestor
    GBE Reptiles and Mammals Have Differentially Retained Long Conserved Noncoding Sequences from the Amniote Ancestor D.E. Janes*,1, C. Chapus2, Y. Gondo3, D.F. Clayton4, S. Sinha4,5, C.A. Blatti5, C.L. Organ1, M.K. Fujita1, C.N. Balakrishnan4, and S.V. Edwards1 1Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 2UMR-MD3, Institut de Recherche Biome´ dicale des Arme´ es, Marseille, France 3RIKEN BioResource Center, Tsukuba, Japan 4Institute for Genomic Biology, University of Illinois at Urbana-Champaign 5Department of Computer Science, University of Illinois at Urbana-Champaign *Corresponding author: E-mail: [email protected] Accepted: 15 December 2010 Abstract Many noncoding regions of genomes appear to be essential to genome function. Conservation of large numbers of noncoding sequences has been reported repeatedly among mammals but not thus far among birds and reptiles. By searching genomes of chicken (Gallus gallus), zebra finch (Taeniopygia guttata), and green anole (Anolis carolinensis), we quantified the conservation among birds and reptiles and across amniotes of long, conserved noncoding sequences (LCNS), which we define as sequences 500 bp in length and exhibiting 95% similarity between species. We found 4,294 LCNS shared between chicken and zebra finch and 574 LCNS shared by the two birds and Anolis. The percent of genomes comprised by LCNS in the two birds (0.0024%) is notably higher than the percent in mammals (,0.0003% to ,0.001%), differences that we show may be explained in part by differences in genome-wide substitution rates. We reconstruct a large number of LCNS for the amniote ancestor (ca.
    [Show full text]