Datasheet Version: 1.0.0 Revision date: 05 Nov 2020

Inhibitor of Growth 3 (ING3) Antibody Catalogue No.:abx004471

Western blot analysis of extracts of various cell lines, using ING3 antibody (abx004471) at 1/1000 dilution.

Immunofluorescence analysis of A549 cells using ING3 antibody (abx004471). Blue: DAPI for nuclear staining.

ING3 Antibody is a Rabbit Polyclonal antibody against ING3. The protein encoded by this is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in involved in remodeling. This gene can activate trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Two alternatively spliced transcript variants encoding different isoforms have been observed.

Target: ING3

Clonality: Polyclonal

Reactivity: Human, Mouse, Rat

Tested Applications:ForWB, IF/ICC Reference Only

Host: Rabbit

Recommended dilutions: WB: 1/500 - 1/2000, IF/ICC: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user.

Conjugation: Unconjugated

Immunogen: Recombinant protein of human ING3.

v1.0.0 Abbexa Ltd, Cambridge, UK · Phone: +44 1223 755950 · Fax: +44 1223 755951 1 Abbexa LLC, Houston, TX, USA · Phone: +1 832 327 7413 www.abbexa.com · Email: [email protected] Datasheet Version: 1.0.0 Revision date: 05 Nov 2020

Isotype: IgG

Form: Liquid

Purification: Affinity purified.

Storage: Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

UniProt Primary AC: Q9NXR8 (UniProt, ExPASy)

Gene Symbol: ING3

GeneID: 54556

String: 9606.ENSP00000320566

Molecular Weight: Calculated MW: 11 kDa, 46 kDa Observed MW: 50 kDa

Buffer: PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.

Concentration: > 1 mg/ml

Note: This product is for research use only.

For Reference Only

v1.0.0 Abbexa Ltd, Cambridge, UK · Phone: +44 1223 755950 · Fax: +44 1223 755951 2 Abbexa LLC, Houston, TX, USA · Phone: +1 832 327 7413 www.abbexa.com · Email: [email protected]