Herbicide Weed Control – Induced Differential Tolerance and Productivity in Cowpea Plants

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Herbicide Weed Control – Induced Differential Tolerance and Productivity in Cowpea Plants 865 Bioscience Journal Original Article HERBICIDE WEED CONTROL – INDUCED DIFFERENTIAL TOLERANCE AND PRODUCTIVITY IN COWPEA PLANTS RESPOSTA DIFERENCIAL DE TOLERÂNCIA E PRODUTIVIDADE DE FEIJÃO-CAUPI INDUZIDA POR HERBICIDAS PARA CONTROLE DE PLANTAS DANINHAS Fernando Ramos de SOUZA1; Ernandes Silva BARBOSA2; Lucas César MARTINS3; Junior BORELLA4; Aroldo Ferreira Lopes MACHADO5 1. Doutorando PPG Fitotecnia, Universidade Federal Rural do Rio de Janeiro – UFRRJ, Seropédica, RJ, Brasil. 2. Mestrando PPG Fitotecnia, Universidade Federal Rural do Rio de Janeiro – UFRRJ, Seropédica, RJ, Brasil. 3. Graduando em Agronomia, Universidade Federal Rural do Rio de Janeiro – UFRRJ, Seropédica, RJ, Brasil. 4. Professor Instituto de Ciências Biológicas, Universidade Federal do Rio Grande – FURG, Rio Grande, RS, Brasil. [email protected]. 5. Professor do Departamento de Fitotecnia, Universidade Federal Rural do Rio de Janeiro – UFRRJ, Seropédica, RJ, Brasil. [email protected] ABSTRACT: The use of herbicides is a represents an efficient way to control the infesting plant population, since it is associated with low operational cost, and it does not demand large amounts of labour. An obstacle to growing cowpea (Vigna unguiculate) is the absence of herbicides registered for this crop. The objective of this work was to evaluate the tolerance of cowpea to herbicides. The experiment was carried out in a randomised block with treatment controls with and without weeding, Bentazon (720 g ha-1), Fluazifop-p-butyl (250 g ha-1), Fomesafen and a tank mix between Fluazifop-p-butyl + Fomesafen (250 + 187.5 g ha-1) with treatment replicates. The herbicides were applied on vegetative (V3 – stage) cowpea plants using a CO2- pressurised backpack sprayer with four spray tips TT 110.02 operating at a pressure of 2.5 bar and applying 240 litres of syrup per hectare. The cultivar BRS Guariba, with five planting lines per plot, was tested with the three central lines of the useful area, scoring 0.5 m of the ends. The species Oxalis latifolia was difficult to control. The Fluazifop-p-butyl + Fomesafen mixture presented better control of weeds. The herbicides caused phytotoxicity to cowpea and reduced leaf area and dry mass. The productivity of the crop was affected due to the low control of weeds. It was concluded that the cowpea presented differential tolerance to the herbicides tested doses. KEYWORDS: Chemical Control. Herbicide tolerance. Productivity. Vigna unguiculata. INTRODUCTION with erect plant architecture, which makes possible the mechanisation of the crop, the cowpea has The cowpea (Vigna unguiculata L. Walp), aroused the interest of large producers in the central- from the Fabaceae family, was first documented in west region in Brazil, mainly in the state of Mato Southeast Asia (PADULOSI, 1997). The plants Grosso (FREIRE FILHO; ROCHA; SILVA, 2011). have a short life cycle (ranging from 60 to 80 days), However, for crops in large areas, one of the low water and nutrient requirements, and it is problems faced by farmers is the need to adopt an reputed to be the most drought- and heat-resistant efficient and low-cost weed management program, crop in semi-arid regions where other food legumes since weed growth in cowpea can result in losses of do not perform as well (SIMPLICIO; yield of up to 95% (FREITAS; MEDEIROS; GONÇALVES; DUARTE, 2016). Such GRANGEIRO, 2009; FAYINMINNU; characteristics make the cowpea the focus of active ADESIYAN; SOSANYA, 2010; GARKO; breeding research for combating poverty in MOHAMMED; FULANI., 2016). developing countries in order to support millions of The use of herbicides represents an efficient people in the tropics and subtropics worldwide way to control weed plant population, due to the low (HALL, 2012). In Brazil, most cowpea productivity operational cost, and because it does not demand is concentrated on the North and Northeast due to large amounts of labour (MONTEIRO; CHAGAS elevated temperatures and water scarcity, making JUNIOR; REIS, 2012). However, the main this crop the main source of plant protein to feed the obstacles to the use of chemical control for this crop needy population (FREITAS, 2014). This makes the is the absence of registered products (SIZENANDO species an interesting model for investigating the FILHO; ALMEIDA; PINTO, 2013). Therefore, it is basis of tolerance adaptation. common the use of registered products for soybeans Currently, with the development of cultivars and common bean in cowpea, considering that the Received: 04/04/19 Biosci. J., Uberlândia, v. 36, n. 3, p. 865-875, May/June 2020 Accepted: 20/12/19 http://dx.doi.org/10.14393/BJ-v36n3a2020-47766 866 Herbicide weed… SOUZA et al. pests, diseases, and weeds that occur in these (250 g ha-1), Fomesafen (187,5 g ha-1), mix of cultures are similar (MONTEIRO; CHAGAS Fluazifop-p-butyl + Fomesafen (250 + 187,5 g ha-1), JUNIOR; REIS, 2012). However, there are currently and two control treatments without herbicide: with no previous studies about the impacts of the and without weeding. For herbicide application, a herbicides on plant development. CO2 pressurised spool was used, operating at a In many cases, the use of herbicides has pressure of 2.5 bars, and previously calibrated to caused several problems to the farmers due to the apply 240 L of syrup per ha. The spray bar was phytotoxicity to plants, causing the stoppage of equipped with four model TT 110.02 tips. The growth and consequently reduction in crop application was performed in the morning, with a productivity (FREITAS; MEDEIROS; recorded temperature of 20°C, relative humidity of GRANGEIRO, 2009). It can also lead to the death 73%, and minimum and maximum wind speed of of plants (MESQUITA; FREITAS; FREIRE 2.4 and 4.18 km/h, respectively. For the weed FILHO, 2017). On the other hand, increased food control, the first weeding was performed at 20 DAA production is a high priority in many parts of the (days after application), followed by a second world, and this need cannot be met without the use weeding 15 thereafter. of indispensable agricultural inputs, such as pesticides (FAYINMNNU et al., 2010). Given the Phytosociology analysis importance of the growing cowpea cultivation in the Two phytosociological evaluations were Southeast and the use of non-registered products for carried out according to Carvalho (2011). The first the weed control of cowpea plants, the aim of this one was evaluated one day before the application of study was to evaluate the tolerance, development, the herbicides and the second one, 30 days after the and productivity of cowpea plants subjected to post- application. For the evaluations, in each emergence herbicides application. experimental plot, two samplings were established by means of randomly launching a metal square MATERIAL AND METHODS measuring 0.30 m on the sides. At each launch, the weeds shoot inside the square were harvested, and Plant material and growth conditions the individuals were counted and identified by The experiment was carried out in field, family, genus, and species. The samples were then (geographical coordinates: 22º45’08.62’’ S and placed in an oven with forced air ventilation at 65ºC 43º40’28.50’’ W – Seropédica/RJ), at an altitude of for 72 hours until reaching a constant mass. 27 m where the Köppen Aw climate classification Subsequently, the samples were weighed on a type predominates, with wet summers and dry precision scale to quantify the dry matter. winters. The average annual temperature was about 23.9ºC and the average precipitation was 1,213 mm Phytotoxicity analysis in the year 2015 (INMET, 2015). The soil, Phytotoxicity analysis of plants was characterized as Acrisol, was collected at depths up performed at 7, 14, 21, and 28 days after application to 40 cm and submitted to (granulometric) chemical of the herbicides (DAA) by visual evaluation of and physical analyses as follows: pH (in H2O) = 5.5; intoxication of cowpea plants according to the Al exchange (cmolc dm-3) = 0.15; Ca+Mg (cmolc modified EWRC scale (FRANS, 1972). The dm-3) = 6.3; P (mg dm-3) = 20; K (mg dm-3) = 28.5; herbicide efficacy was evaluated for weed control V (%) = 69.6; m (%) = 2.91; Sum of bases (%) = with percentages of monocotyledonous and 6.48. cyperaceous weeds (FE), eudicotyledonous (FL), Plants of cowpea (Vigna unguiculate L. and total control (TOTAL). The absence of control Walp) and cv. BRS Guariba were obtained from was attributed 0% and total control 100%, seeds. The experimental set up consisted of five evidenced by the death of the plants. planting lines per plot, tested with the three central lines of the useful area, scoring 0.5 m of the ends Growing parameters with plenty density of 12 plants per linear m-1. The Cowpea plants at the reproductive stage seeds were inoculated 24 h before sowing with were used to determine leaf area index and dry Bradyrhizobium sp. strain BR 3262 (5 g Kg-1 of weight accumulation. Leaf area was measured in a seeds). portable leaf area meter model LI – 3000C (LI- The treatments were initiated with plants of COR, USA). Afterwards, plants were dried in an cowpea at vegetative stage (V3), 21 days after oven at 65°C with forced air ventilation until getting sowing and consisted of the application of a dry weight constant. herbicides: Bentazon (720 g ha-1), Fluazifop-p-butyl Biosci. J., Uberlândia, v. 36, n. 3, p. 865-875, May/June 2020 http://dx.doi.org/10.14393/BJ-v36n3a2020-47766 867 Herbicide weed… SOUZA et al. Productivity analysis analysed by analysis of variance (ANOVA). When At the end of the reproductive stage, ten F was significant, the treatment means were plants were randomly chosen per line plot to compared by the Tukey test (p ≤ 0.05), except for evaluate productivity: number of pods per plant the phytosociological ones.
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