Microscope Cartoon •A microscope is a tool that is used to magnify the features of an object. •A compound microscope has two or more lenses. Eyepiece
Coarse Focus Objective
Lenses Fine Focus
Stage Clips
Arm Diaphragm
Stage Light Source
Base Parts of a compound microscope: •Eyepiece – what you look into; contains a 10X lens •Objective lenses - three separate lenses that have different powers of magnification (4x, 10x, 40x) •Coarse focus knob - focuses the image under LOW power (4x) •Fine focus knob - focuses the image under HIGH power (10x & 40x) •Stage and stage clips - support and hold the slide in place •Light source - shines light through the slide •Diaphragm - controls the amount of light available •Arm – supports the eyepiece & objective lenses •Base – bottom of the microscope Microscope Magnification Magnification •The eyepiece lens has a magnification of 10x •Each objective lens has a specific magnification (4x, 10x, 40x)
Total magnification = eyepiece x objective lens For Example •If the eyepiece has a magnification of 10X and you are using the 4x objective lens, what is the total magnification? 10 x 4 = 40
**This means the image you see through the eyepiece while using the 4x objective lens is 40 times larger than real life.** How to View a Slide with a Microscope Video Viewing a slide with a microscope
1. Turn on light source 2. Put slide on stage and secure with stage clips 3. Adjust the diaphragm until an adequate amount of light is available. 4. Set the microscope to the lowest power objective lens.(4x) 5. Use the Coarse Focus Knob to bring the image into focus. 6. Change to a higher power objective lenses, if you want to zoom in. (10x then 40x) 7. Use the Fine Focus Knob to bring the image into focus. Microscope Safety •When using 4x objective, use the coarse focus knob •When using 10x and 40x objectives, use ONLY the fine focus knob so that you don’t scratch a lens or break a slide. • Always carry your microscope with one hand under the base and one hand on the
arm. • Some microscopes may have a mirror as the light source. DO NOT aim the mirror
directly at the sun.