CATALOGUE VER 3.2 CONTENTS OF THE CATALOGUE IMPORTANT CONTACTS

Solid media pp. 4 - 11 www.labmediaservis.cz

Media for simultaneous detection p. 11 Orders: +420 702 120 241 Liquid media pp. 12 - 14 [email protected]

Media for cultivation and isolation Technology: of various species of mycobacteria pp. 14 - 15 +420 602 501 256 [email protected] Commissioned preparation of specific antisera p. 16

Dyes and solutions p. 17

MIC kits for IVD p. 18

MIC kits for diagnostics in veterinary medicine p. 19

DTM – Dermatophyte Test Medium p. 20

Diagnostics of American foulbrood of honey bees p. 20

MetriCult p. 21

MicroMast p. 22

PM test (Pure milk test) p. 23

PET test p. 24

Hygiene of food and water – liquid media p. 25

Hygiene of food and water – solid media pp. 26 - 27

2 Dear customers, in March 2017 LabMediaServis s.r.o. celebrated 10 years of ex- Why to buy products made by our company? istence in the Czech market. On the occasion of this important anniversary, we prepared an update of our Catalogue of Products • We are a flexible, adaptable and genuinely Czech company and Services. whose priority is a satisfied customer but, above all, we are a family company which has a prospective continuation in the In this version, you will find an extended assortment of prod- next young generation of co-owners. ucts for in vitro diagnostics in microbiology laboratories. They • Our products are subject to careful quality control and come are manufactured and distributed according to the established with certification of quality assurance. ISO quality system. • We have certifications for ISO 9001, 14001 and 13485. • We own a herd of accredited rams kept for blood and blood For veterinary customers, items focused on their requirements are derivatives. intended. We can boast the Gold Medal we were awarded on • We deliver the goods using our own refrigerated cars equipped May 2017 at the AnimalTech fair in Brno for the product sold with a temperature curve recorder. under the name PM test. • We cooperate with the Academy of Sciences of the Czech Republic, VRI in Brno, universities and schools of various stages. The last part of the Catalogue is intended for laboratories focused on water and food quality testing.

We made the update based on your requirements and sugges- tions which contributed to the development of further media, solu- tions and blood derivatives.

For more information see www.labmediaservis.cz 3 ALOA BE CIN Listeria Yersinia monocytogenes faecalis enterocolitica

SOLID MEDIA

Product Description Purpose Category Packaging 0,8% 0,8% agar Determination of serotype of in the genus Diagnostic PD90 Chromogenic Listeria Isolation, preliminary identification and enumeration ALOA Selective diagnostic PD90 agar (ALOA) of Listeria spp. and Listeria monocytogenes in samples AMIES Amies Transport medium General TT Antimycotic sensitivity Antimycotic susceptibility testing using disc AS General PD90 agar diffusion method Selective isolation and preliminary identification BE Selective diagnostic PD90, TT of group D streptococci and enterococci Selective isolation and preliminary identification BEA Bile esculin azide agar Selective diagnostic PD90, TT of group D streptococci and enterococci Bordet Gengou agar Cultivation and isolation of Bordetella pertussis BG General PD90 20 % BDK and Bordetella parapertussis Bordet Gengou agar Selective isolation of Bordetella spp., especially BGATB Selective PD90 20 % BDK + ATB Bordetella pertussis and Bordetella parapertussis BHIA BHI agar Isolation and cultivation of a wide range of microorganisms General PD90 Selective isolation, identification and enumeration BP Baird-Parker agar Selective diagnostic PD90 of Staphylococus aureus in samples Isolation and cultivation of Brucella spp. and BRA other fastidious microorganisms from clinical and General PD90 other samples Brucella agar with hemin Isolation and cultivation of demanding bacteria such BRAHK General PD90 and vitamin K as Brucella spp. and other anaerobes CAS Casitone agar Isolation and cultivation of yeasts and fungi General PD90 Clostridium difficile CD Selective isolation and cultivation of Clostridium difficile Selective PD90 blood agar Selective isolation and enumeration of Yersinia CIN CIN – Yersinia agar Selective PD90 enterocolitica in samples CJC for cultivation of Selective isolation of Campylobacter spp. Selective PD90 Campylobacter jejuni Agar for cultivation of CJK Campylobacter jejuni Selective isolation of Campylobacter spp. Selective PD90 (KARMALI) Blood agar for cultivation Selective isolation of Campylobacter spp. CJPB of Campylobacter jejuni Selective PD90 at 35°C and 42°C (BUTZLER)

4 = Chromogenic media; PD90 = Petri Dish ∅ 90 mm; PD55 = Petri Dish ∅ 55 mm CJPBW CLED COK Campylobacter Proteus Staphylococcus jejuni vulgaris aureus

SOLID MEDIA

Product Description Purpose Category Packaging Blood agar for cultiva- Selective isolation of Campylobacter spp. with CJPBW tion of Campylobacter Selective PD90 simultaneous inhibition of Candida albicans jejuni (BLASER-WANG) Blood agar for cultivation CJPS of Campylobacter jejuni Selective isolation of Campylobacter spp. Selective PD90 (SKIRROW) CLED (cystine lactose For a number of aerobic G+, G– bacteria, preliminary CLED electrolyte deficient) identification of several genera of bacteria based on General PD90 medium their ability to ferment lactose Identification and enumeration of Pseudomonas CN Pseudomonas agar Selective PD90 aeruginosa in samples using membrane filter technique Cultivation and isolation of fastidious microorganisms, COK Chocolate agar General PD90 in particular bacteria of the genus Haemophilus Chocolate blood agar Selective isolation and cultivation of fastidious micro­ COKBAC Selective PD90 with bacitracin organisms, especially bacteria of the genus Haemophilus Chocolate agar with Selective isolation and cultivation of fastidious micro­ COKVAN Selective PD90 vancomycin organisms, especially bacteria of the genus Haemophilus Clostridium perfringens Preliminary identification and enumeration CP Selective PD90 agar of Clostridium perfingens in samples CZADOX Czapek – Dox agar Cultivation of filamentous fungi, fungi and yeasts General PD90 Deoxycholate citrate Selective isolation and differentiation of G- bacteria, DC Selective diagnostic PD90 agar especially sulphane-producing enterobacteria Detection of deoxyribonuclease- DNASE DNase agar Diagnostic PD90 producing bacteria DRBC agar with dichlo- Selective isolation and enumeration of yeasts DRBC rane, Bengal red and Selective PD90 and fungi in samples chloramphenicol Dermatophyte Test Selective isolation and detection DTM Selective diagnostic PD55 Medium of dermatophytes and yeast

ENDO agar with Preliminary identification of EBK Diagnostic PD90 biochemical wedge and some G-, nonfermenting rod-shaped bacteria

Selective isolation and cultivation of Streptococcus EDW Edwards agar Selective PD90 agalactiae and other streptococci

TT = Test Tube; V = Vial; C6 = 6-compartment For more information see www.labmediaservis.cz 5 EBK ESIA GREENB Escherichia Enterobacter (Cronobacter) Escherichia coli sakazakii coli

SOLID MEDIA

Product Description Purpose Category Packaging Edwards agar with colistin Selective isolation and cultivation of Streptococcus EDWM Selective diagnostic PD90 and oxolinic acid agalactiae Detection of enterobacteria and some PD90, ENDO Selective diagnostic G- non-fermenting rod-shaped bacteria PD55 Chromogenic Enterobacter Selective isolation, differentiation and enumeration ESIA Selective diagnostic PD90 sakazakii agar of Enterobacter sakazakii in samples GA Glucose agar Isolation of a wide range of microorganisms General TT

GBS GBS agar Isolation and detection of group B streptococci Selective diagnostic PD90 Isolation and cultivation of fastidious bacteria GO GO blood agar General PD90 such as Neisseria spp. GO blood agar with Selective isolation and cultivation of fastidious bacteria GOATB Selective PD90 such as Neisseria spp. GREENB Brilliant green agar Selective isolation and cultivation of Salmonella spp. Selective PD90 Differentiation of microorganisms based on fermentation of HAJN Hajn agar Selective diagnostic TT glucose, lactose and sucrose, and production of sulphane Chocolate agar for cultiva- Selective isolation and cultivation HPC Selective PD90 tion of Helicobacter pylori of Helicobater pylori Blood agar for cultivation HPK Selective isolation and cultivation of Helicobater pylori Selective PD90 of Helicobacter pylori Haemophilus test Susceptibility testing of fastidious bacteria (Haemophilus HTM General PD90 medium spp.) using the disk diffusion method according to CLSI Selective isolation and preliminary identification Chromogenic CHCA of clinically relevant species of Candida spp. Selective diagnostic PD90 Candida agar (C. albicans, C. tropicalis, C. krusei, C. glabrata…) Chromogenic Simultaneous detection of coliform bacteria CHCLT Selective diagnostic PD90, V Coliform agar and Escherichia coli Chromogenic Selective isolation, identification and enumeration CHECXG Selective PD55 E. coli x – gluc agar of E. coli in water samples Chromogenic Identification and differentiation of Salmonella spp. CHSA Selective diagnostic PD90 Salmonella agar from other species of enterobacteria IND Indole Detection of tryptophanase-producing bacteria Diagnostic TT Columbia blood Isolation and cultivation of non-fastidious and fastidious PD90, PCA, KA5 General agar with BDK 5% microorganisms from clinical and other samples P12x12

6 = Chromogenic media; PD90 = Petri Dish ∅ 90 mm; PD55 = Petri Dish ∅ 55 mm KACNA MC KA5 Streptococcus Salmonella Streptococcus pyogenes enteritidis pyogenes

SOLID MEDIA

Product Description Purpose Category Packaging Columbia blood agar Isolation and cultivation of both non-fastidious KA7 General PD90 with BDK 7% and fastidious microorganisms Columbia CNA blood Selective isolation and cultivation KACNA Selective PD90 agar of gram-positive bacteria Columbia blood agar KAN Selective isolation and cultivation of staphylococci Selective PD90 with 5% NaCl Columbia blood agar KAN10 Selective isolation and cultivation of staphylococci Selective PD90 with 10% NaCl Screening chromogenic medium for detection and Chromogenic KPC differentiation of carbapenemase (CRE+) gram-negative Selective PD90 KPC agar bacteria Legionella BCYE agar LEGBCYE Isolation of Legionellaceae General PD90 with L-cysteine Legionella BCYE agar Control medium for LEGBCYE medium; LEGBYE Selective PD90 without L-cysteine Legionellaceae do not grow on this medium Selective isolation and cultivation of bacteria LEGV Legionella GVPC agar Selective PD90 in the genus Legionella Selective isolation and cultivation LIDSKA Agar with human blood Selective PD90 of Gardnerella vaginalis Selective isolation and cultivation of staphylococci MANS Selective diagnostic PD90 from clinical and other samples Detection of enterobacteria and some MC MacConkey agar Selective PD90 G-, non-fermenting rod-shaped bacteria Detection of enterobacteria and some MC3 MacConkey agar No.3 Selective PD90 G-non-fermenting rod-shaped bacteria MCL McLeod agar Cultivation and isolation of Francisella tularensis General PD90 Campylobacter blood- Selective isolation and cultivation of Campylobacter spp., MCCDA free selective medium Selective PD90 especially C. jejuni, C. coli and C. laridis (modified CCDA - Preston) Selective isolation and enumeration of Clostridium MCP M-CP agar perfringens in samples using a membrane filter Selective PD55 technique MacConkey agar with Selective isolation and detection MCS Selective PD90 sorbitol of Escherichia coli O157:H7

TT = Test Tube; PCA = ; P12x12 = Plate 120x120 mm For more information see www.labmediaservis.cz 7 MH MH MHKREV10 Escherichia Pseudomonas Streptococcus coli aeruginosa pneumoniae

SOLID MEDIA

Product Description Purpose Category Packaging Detection and enumeration of coliform bacteria M-FC M-FC agar Selective PD90, V in samples using membrane filter technique MH Mueller Hinton agar Susceptibility testing of bacteria General PD90 Mueller Hinton agar Detection of extended-spectrum β-lactamase-producing (ESBL) MHOXA General PD90 with oxacillin bacteria with simultaneous production of AmpC β-laktamázy Mueller Hinton agar Susceptibility testing of fastidious bacteria (Streptococcus MHKREV10 General PD90 with 10% ram blood spp. etc.) using the disk diffusion method according to CLSI Mueller Hinton agar Susceptibility testing of fastidious bacteria (Streptococcus PD90, MHKREV5 General with 5% ram blood spp. etc.) using the disk diffusion method according to CLSI P12x12 Mueller Hinton agar Determination of yeast susceptibility to antifungal MHG with 2% glucose agents using the disk diffusion method according General PD90 and methylene blue to the CLSI standard Susceptibility testing of fastidious bacteria such as Mueller Hinton agar Streptoccocus spp., Haemophilus spp., Campylobac- MHF with horse blood General PD90 ter spp. etc., to antibiotics using the EUCAST disc and NAD diffusion method Detection, isolation and enumeration of yeasts MEA Malt Extract agar Selective PD90 and fungi in samples Selective isolation and cultivation of fastidious bacteria MLE Martin Lewis agar Selective PD90 such as Neisseria spp. MIU – Differentiation of bacteria based on their ability to move, MIU Diagnostic TT Motility Indole Urea urease production and indole production MRS MRS agar Isolation, cultivation and determination of in samples Selective PD90, V Selective isolation of methicillin-resistant Staphylococcus MRSA MRSA agar Selective PD90 aureus (MRSA) MSRVL MSRV agar Detection of motile bacteria of Salmonella species Selective diagnostic V Mannitol egg yolk MYP Selective isolation and identification of Bacillus cereus Selective PD90 polymyxin agar Isolation and cultivation of non-fastidious NTA General PD90, V microorganisms Oxacillin resistance Selective methicillin-resistant Staphylococcus aureus ORSAB Selective PD90 screening agar (MRSA) medium 1st generation (supplier OXOID) Selective isolation of methicillin-resistant Staphylococcus OXA Oxacillin agar Selective PD90 aureus (MRSA)

8 PD90 = Petri Dish ∅ 90 mm; PD55 = Petri Dish ∅ 55 mm MHKREV10 MHKREV10 ORSAB SAB Streptococcus Escherichia Staphylococcus Candida pneumoniae coli aureus MRSA albicans

SOLID MEDIA

Product Description Purpose Category Packaging OXF Oxford Listeria agar Selective isolation and detection of Listeria monocytogenes Selective PD90 PALCAM Palcam Listeria agar Selective isolation and detection of Listeria monocytogenes Selective diagnostic PD90 Plate count agar Isolation, cultivation and enumeration of total number PD90, PCA, PCA General (PCA / GTK) of microorganisms in samples V Blood agar for cultivation Selective isolation and cultivation of Campylobacter PRA of Campylobacter jejuni jejuni and Campylobacter coli from clinical and other Selective PD90 (PRESTON) samples Modified Identification and differentiation of Salmonella spp. RAM Selective diagnostic PD90 Rambach agar from other species of Enterobacteriaceae Selective identification and differentiation of Candida albicans RICE Rice agar Selective PD90 from other Candida species based on chlamydospore production SAB Isolation and cultivation of yeasts, fungi and acidophilic bact. General PD90, TT, V Sabouraud agar 2 (with SAB2 Selective isolation and cultivation of yeasts and fungi Selective PD90, TT chloramphenicol – CMP) Sabouraud agar 3 (with chloramphenicol – SAB3 Selective isolation and cultivation of yeasts and fungi Selective PD90, TT CMP and cycloheximide – CHM) Sabouraud agar 4 (with chloramphenicol – SAB4 Selective isolation and cultivation of yeasts and fungi Selective PD90 CMP and gentamicin -– GEN) Sabouraud agar Testing the efficiency of sanitation of equipment SDTL with 0.3% Lecithin General V surfaces, boxes, water-miscible cosmetics etc. and 3% Tween 80 Isolation, detection and enumeration of enterococci PD90, SBA Slanetz-Bartley agar Selective in samples by membrane filter technique PD55, V Isolation and differentiation of bacteria in the family SC Simmons citrate agar Selective diagnostic TT Enterobacteriaceae based on the ability to utilize citrate Schaedler anaerobic Isolation and cultivation of aerobic SCH General PD90 agar with 5% BDK and anaerobic bacteria Selective isolation and cultivation of anaerobic SCHCNA Schaedler CNA agar Selective PD90 gram-positive bacteria from clinical and other samples Isolation and cultivation strictly anaerobic SCHKV Schaedler KV agar Selective PD90 gram-negative bacteria

TT = Test Tube; PCA = Plate Count Agar; V = Vial; P12x12 = Plate 120x120 mm For more information see www.labmediaservis.cz 9 SS SCH SS Salmonella Bacteroides enteritidis fragilis flexneri

SOLID MEDIA

Product Description Purpose Category Packaging Isolation, cultivation and preservation of SIKA Slant agar General TT a wide range of microorganisms

SIM (Sulfide indole Differentiation of Enterobacteriaceae based SIM Diagnostic TT motility) medium on sulphane and indole production, and motility

Salmonella Selective detection of genus SS Selective PD90 Shigella agar Salmonella spp. and Shigela spp.

Chromogenic Isolation and preliminary identification of group B strepto- STREPB Selective diagnostic PD90 Strepto B agar cocci (Streptococcus agalactiae) from clinical samples TCBS TCBS agar Selective isolation and cultivation of the genus Vibrio Selective PD90 Selective isolation and identification of Corynebacterium TINSD Tinsdale agar Selective diagnostic PD90 diptheriae, the agar is free of blood

Selective isolation of Trichomonas spp. TM Trichomonas medium Selective TT and Candida spp.

Cultivation of a wide range of microorganisms PD90, PCA, TSA Tryptone-soya agar General and environment sterility testing V Tryptone-soya agar TSANBDK Cultivation of β-haemolytic streptococci Selective PD90 with NaCl and 5% BDK

Tryptone-soya agar TSA5BDK Cultivation of non-fastidious and fastidious bacteria General PD90 with 5% BDK

Tryptose Sulphite Isolation and enumeration of Clostridium perfringens TSC Selective diagnostic PD55 D-cycloserine agar in drinking water

Tryptone-soya agar Testing the efficiency of sanitation of equipment TSTL with 0.3% Lecithin General V surfaces, boxes, water-miscible cosmetics etc. and 3% Tween 80 PD90, TWEEN Tween agar Cultivation of a wide variety of microorganisms General PD55

Differentiation of microorganisms according UREA Urea agar Diagnostic TT to their ability to produce urease

Preliminary identification and differentiation of microor- PD90, UTIB UTI Brilliance agar Selective diagnostic ganisms causing e.g. urinary tract infections PD55

10 = Chromogenic media; PD90 = Petri Dish ∅ 90 mm; PD55 = Petri Dish ∅ 55 mm VRBG D6KAMC P2MCXLD Escherichia Destičky 3+3 Salmonella coli KA + MC enteritidis

SOLID MEDIA

Product Description Purpose Category Packaging Selective isolation, detection and enumeration of bacteria VRBG VRBG agar Selective PD90, PCA, V from the family Enterobacteriaceae in samples

Selective isolation, detection and enumeration VRBL VRBL agar Selective PD90, V of coliform bacteria in samples

Wilkins-Chalgren anaero- Isolation and cultivation of aerobic and anaerobic WC5BDK General PD90 bic agar with 5% BDK bacteria

Xylose Lysine Selective isolation and cultivation of Enterobacteriaceae, XLD Selective diagnostic PD90 Deoxycholate agar including the genus Salmonella and Shigella

Selective isolation and identification of yeasts and fungi YGC YGC (GKCH) agar Selective PD90 in samples

MEDIA FOR SIMULTANEOUS DETECTION

Product Description Category Packaging

D6CHI2 Plates 3+3 COKO Hi-2 Selective C6

D6KAMC Plates 3+3 KA + MC General + Selective diagnostic C6

D6KMS Plates 3+2+1 KA + MC + SBA General + Selective diagnostic C6

P2KA5KACNA 2-compartment Petri dish KA5/KACNA General + Selective PD90 - 2-comp.

P2MCKACNA 2-compartment Petri dish MC/KACNA Selective PD90 - 2-comp.

P2MCXLD 2-compartment Petri dish MC/XLD Selective diagnostic PD90 - 2-comp.

TT = Test Tube; PCA = Plate Count Agar; V = Vial For more information see www.labmediaservis.cz 11 LIQUID MEDIA

Product Description Purpose Category Packaging ACD Acetamide broth Confirmation of Selective diagnostic TT, V Alkaline peptone Isolation, cultivation and propagation APV Selective TT, V water of bacteria of Vibrio genus BB Bolton broth Selective isolation and enrichment of Campylobacter spp. Selective TT, V Multiplication of a wide range of bacteria BHI BHI broth General TT, V from clinical and other samples Dey-Engley Testing of disinfectants and determination DENB General TT, V neutralizing broth of their antibacterial activity FP Phosphate buffer Dilution of clinical and other samples Diluent TT, V FR Saline solution Dilution of clinical and other samples Diluent TT, V FRB Fraser broth Selective isolation and enrichment of Listeria spp. Selective TT, V FRB1/2 Fraser broth 1/2 Selective isolation and enrichment of Listeria spp. Selective TT, V Saline solution FRG Dilution of clinical and other samples Diluent TT, V with 1% glucose phosphate buffered FRP Dilution of clinical and other samples Diluent TT, V saline solution saline solution for FRPB Dilution of clinical and other samples Diluent TT, V Branhamella spp. Saline peptone FRPP Dilution of clinical and other samples Diluent TT, V water Glucose Cultivation of a wide range of microorganisms, GB General TT, V broth including more demanding bacteria Detection and enumeration of coagulase-positive GLCT Giolitti-Cantoni broth Selective TT, V staphylococci in samples Multiplication of a wide range of bacteria JB Liver broth General TT, V from clinical and other samples LAK Lactobroth Detection of coliform bacteria Diagnostic TT, V Lactose-Sulphite Selective isolation and enumeration LS Selective TT, V broth of Clostridium perfringens

12 TT = Test Tube; V = Vial LIQUID MEDIA

Product Description Purpose Category Packaging Decontamination solution for processing of sam- LST Lauryl sulphate Solution TT, V ples suspected of containing mycobacteria Detection of lysine-decarboxylase production for ex- LYZ Lysine Diagnostic TT, V ample, in bacteria in the family Enterobacteriaceae Mueller Hinton Testing of susceptibility of bacteria to antibiotics MHB General TT, V broth by MIC microdilution method Muller Kauffman MKTTN tetrathionate broth Selective isolation of Salmonella spp. Selective TT, V with novobiocin Modified tryptose broth MLST Selective enrichment of Enterobacter sakazakii Selective TT, V with lauryl sulphate MOSEL EE broth, Mossel Selective enrichment of Enterobacteriaceae Selective TT, V

ORN Ornithine Detection of ornithine decarboxylation Diagnostic TT, V

Peptone medium PEPLAK Detection of coliform bacteria Diagnostic TT, V with lactose PV Peptone water Dilution of clinical and other samples Diluent TT, V RVS broth Selective enrichment of bacteria, RVS (Rappaport Vassiliadis Selective TT, V e.g. of the genus Yersinia, Salmonella soya broth) SABB Sabouraud broth Enrichment of filamentous fungi, moulds and yeasts General TT, V Selective isolation and enrichment of bacteria of the SEL Selenite broth Selective TT, V genus Salmonella from clinical and other samples Selective isolation and enrichment of bacteria of SELCYS Selenite cysteine Selective TT, V the genus Salmonella from clinical and other samples Tryptone-soya agar Testing the efficiency of sanitation of equipment TBTL with 1% Lecithin General TT, V surfaces, boxes, water-miscible cosmetics etc. and 1% Tween 80

For more information see www.labmediaservis.cz 13 LIQUID MEDIA

Product Description Purpose Category Packaging Medium without resazurin to control the purity Thioglycolate TH and sterility, particularly of turbid and viscous General TT, V broth products Isolation and cultivation of a wide range of – microorganisms (aerobic and anaerobic bacteria) THB General TT, V BREWER and for sterility control of solutions containing a mercury-based­ preservative

Thioglycolate broth Medium with resazurin to control the purity and THR General TT, V with resazurin sterility, particularly of turbid and viscous products

Cultivation of group A haemolytic streptococci TOD Todd-Hewitt broth General TT, V before serotyping Buffered peptone Multiplication of bacteria, mainly pathogenic TPV General TT, V water Enterobacteriaceae Enrichment of a wide range of bacteria from TSB Tryptone-soya broth General TT, V clinical and other samples UR Urease test Determination of urease production Diagnostic TT, V Isolation, cultivation and propagation of anaerobic VL VL broth General TT, V bacteria from clinical and other samples

MEDIA FOR CULTIVATION AND ISOLATION OF VARIOUS TYPES OF MYCOBACTERIA

Product Description Packaging ARYL Arylsulfatase TT TWEENZK Tween liquid medium in a test tube TT SFM Sauton medium V SUL Sula medium TT, V

SULNANO3 Sula medium with NaNO3 TT, V SUL10x Sula medium 10x concentrated V

14 TT = Test Tube; V = Vial; PD90 = Petri Dish ∅ 90 mm MEDIA FOR CULTIVATION AND ISOLATION OF VARIOUS TYPES OF MYCOBACTERIA

Product Description Packaging Purpose OG Ogawa egg medium TT Cultivation of mycobacteria from clinical samples Isolation and cultivation of Mycobacterium LJ Löwenstein-Jensen egg medium TT spp. from clinical and other samples

LJA Löwenstein-Jensen egg medium with antituberculotics TT

LJAMP Löwenstein-Jensen egg medium with antituberculotics AMP TT

LJAMP-PM Löwenstein-Jensen egg medium with antituberculotics AMP PD90

LJCYC40 Löwenstein-Jensen egg medium with antituberculotics CYC40 TT

LJEMB2 Löwenstein-Jensen egg medium with antituberculotics EMB2 TT

LJETI40 Löwenstein-Jensen egg medium with antituberculotics ETI40 TT

LJHA Löwenstein-Jensen egg medium with antituberculotics HA TT

LJINH02 Löwenstein-Jensen egg medium with antituberculotics INH0,2 TT Löwenstein-Jensen egg media with antituber- LJINH1 Löwenstein-Jensen egg medium with antituberculotics INH1 TT culotics are used for selective isolation and LJINH10 Löwenstein-Jensen egg medium with antituberculotics INH10 TT cultivation of atypical mycobacteria from clinical and other samples and susceptibility LJKAN30 Löwenstein-Jensen egg medium with antituberculotics KAN30 TT testing of mycobacteria to antituberculotics LJP-AMIN Löwenstein-Jensen egg medium with antituberculotics P-AMIN TT

LJPZA0 Löwenstein-Jensen egg medium with antituberculotics PZA0 TT

LJPZA400 Löwenstein-Jensen egg medium with antituberculotics PZA400 TT

LJRIF40 Löwenstein-Jensen egg medium with antituberculotics RIF40 TT

LJSTM3 Löwenstein-Jensen egg medium with antituberculotics STM3 TT

LJSTM4 Löwenstein-Jensen egg medium with antituberculotics STM4 TT

LJSTM5 Löwenstein-Jensen egg medium with antituberculotics STM5 TT

LJTCH10 Löwenstein-Jensen egg medium with antituberculotics TCH10 TT

For more information see www.labmediaservis.cz 15 COMMISSIONED PREPARATION OF SPECIFIC ANTISERA We can prepare specific sheep and rabbit antisera according to the customer‘s needs and wishes. Immunization is performed with antigens supplied by the customer according to the immunization schedule required by the customer. If the customer does not have their preferred immunization schedule, we will offer our own proven schedules.

DEFIBRINATED BLOOD BLOOD SERUM Product Description Product Description BDK Defibrinated sheep blood BES Sheep serum HDK Defibrinated bovine blood HOS Bovine serum KODK Defibrinated horse blood KOS Horse serum KDK Defibrinated rabbit blood KRS Rabbit serum MOS Guinea pig serum BLOOD CELLS TES Veal precolostral serum Product Description BA Sheep blood cells BLOOD PLASMA HA Bovine blood cells Product Description KOA Horse blood cells BEP Sheep plasma KA Rabbit blood cells HOP Bovine plasma KRP Rabbit plasma

16 DYES AND SOLUTIONS

Product Description Product Description B-BTM Bromothymol blue B-MWT Weber‘s modified trichrome B-FC02 Phenol red 0.2% R-A Solution A (4% NaOH) B-FC04 Phenol red 0.4% R-AB96 96% Alcoholbenzine B-KAL Acidic alcohol for fluorescence R-AB80 80% Alcoholbenzine B-KALZN Acidic alcohol for Ziehl-Neelsen R-ACCY N-acetyl-L-cystein B-KBF Carbol-Fuchsin R-B Solution B (2.9% sodium citrate) B-KBFTBC Carbol-Fuchsin for TBC R-C Solution C (5% Tween) B-KC004 Cresol red 0.04% R-D Solution D (Sőrensen's buffer) B-KC04 Cresol red 0.4% R-E Solution E B-KV Crystal violet R-HI Heidenhain I B-LR Lugol's solution R-HII Heidenhain II B-MTV Methyl violet R-IIR Reagent II B-MV Dye according to Miláček and Vítovec R-IR Reagent I B-MZ1 Malachite green 1% R-KATO KATO solution B-MZ2 Malachite green 2% R-KX Carboxylol B-MZ5 Malachite green 5% R-SA Sublimate-alcohol B-OG Orange G R-X Xylol

For more information see www.labmediaservis.cz 17 MIC KITS FOR IVD in clinical laboratories Laboratory in vitro determination of susceptibility of bacteria by microdilution method.

Penicillin, oxacillin, ampicillin/sulbactam in 2/1 ratio; chloramphenicol, MIC determination kit – Staphylococcus spp. tetracycline, vancomycin, erythromycin, clindamycin, ciprofloxacin, gentamicin, teicoplanin, trimethoprim/sulfamethoxazole in 1/19 ratio.

Penicillin, ampicillin, tigecycline, gentamicin, chloramphenicol, MIC determination kit – Enterococcus spp. tetracycline, erythromycin, ciprofloxacin, vancomycin, teicoplanin, linezolid, nitrofurantoin.

Piperacillin, piperacillin/tazobactam (fixed concentration of tazobactam MIC determination kit – Pseudomonas spp. 4 mg/l), aztreonam, meropenem, ceftazidime, cefoperazone, cefepime, gentamicin, amikacin, colistin, ofloxacin, ciprofloxacin.

Ampicillin, ampicillin/sulbactam 2/1, cefazolin, cefuroxime, cefoxitin, MIC determination kit Gˉ I chloramphenicol, tetracycline, gentamicin, trimethoprim/sulfamethoxazole 1/19, colistin, ofloxacin, aztreonam.

Amikacin, piperacillin / tazobactam, cefoperazone, cefotaxime, MIC determination kit Gˉ II ceftazidime, cefepime, cefoperazone / sulbactam 2/1, meropenem, ciprofloxacin, tigecycline, tobramycin, piperacillin.

We manufacture and distribute products in cooperation with:

Veterinary Research Institute Hudcova 296/70 621 00 Brno www.vri.cz

18 MIC KITS FOR DIAGNOSTICS IN VETERINARY MEDICINE Kits for MIC determination in bacterial path- Variants (a), (b), (c) differ between the kits according to the medium used ogens with veterinary importance. for the growth of the strains examined.

Antibiotics are commonly used to treat bac- • Kit for MIC detection in porcine • Kit for MIC detection in pathogens terial infections in humans and animals. respiratory and enteral pathogens with veterinary importance Their improper use can lead to resistance of a) for nutritionally undemanding bacteria a) for nutritionally undemanding bacteria some bacteria to antimicrobials or spread of b) for Streptococcus spp. b) Streptococcus spp. resistant populations of bacteria. c) for Actinobacillus pleuropneumoniae c) for Actinobacillus pleuropneumoniae, Histophilus somni To initiate effective antimicrobial treatment of bacterial infections, the following are im- • Kit for MIC detection in respiratory portant: and enteral bovine pathogens • Kit for MIC detection in mastitis • Correct identification of the causative a) for nutritionally undemanding bacteria pathogens agent of the bacterial disease b) for Streptococcus spp. a) for nutritionally undemanding bacteria • Choice of antimicrobial for treatment c) for Histophilus somni b) for nutritionally demanding bacteria based on susceptibility or resistance testing of the agent to the antimicrobial candidate for therapy of the infection. • Kit for MIC detection in poultry • Kit for MIC detection in bacterial pathogens pathogens of dogs and cats One of the recommended methods for test- a) for nutritionally undemanding bacteria a) nutritionally undemanding bacteria ing resistance or susceptibility of bacteria is b) for nutritionally demanding bacteria b) pro nutritionally demanding bacteria determination of minimum inhibitory concen- trations (MIC) of antimicrobials. • Kit for MIC detection in nutrition- The kits contain antimicrobials that are used ally undemanding Gram-negative to treat animals, including those used only bacteria in veterinary medicine. The proportion of antimicrobial agents in each kit is original and corresponds either to the agents used • Kit for MIC detection in nutrition- to treat bacterial infections according to the ally undemanding Gram-positive target animal species (pigs, cattle, poultry) bacteria or selected according to pathogen species a) for nutritionally undemanding bacteria (G-, G+ or specific for veterinary medicine). (Staphylococcus spp., Enterococcus spp.) b) for nutritionally demanding bacteria (Streptococcus spp.)

For more information see www.labmediaservis.cz 19 DTM MYPGNP Trychophyton metagrophytes Paenibacillus larvae

DTM – DERMATOPHYTE TEST MEDIUM Dermatophyte Test Medium (DTM) according to Taplin is a selective and differentiation medium designed to identify dermatophytes such as Trichophyton, Microsporum and Epidermatophyton from swabs of wounds, skin, hair, ears, etc.

DTM advantages: • Commercially available • Rapid reaction • Easy to use • Inhibition of the growth of a wide range of bacteria and the majority of saprophytic fungi • Easy way to high quality results

Presentation: A set of 5 Petri dish ∅ 55 mm packed in a paper box.

DIAGNOSTICS OF AMERICAN FOULBROOD OF HONEY BEES

Media used for isolation and cultivation of Paenibacillus larvae from samples of honey, debris, wax, etc. MYPGP MYPGP agar MYPGPn MYPGP agar enriched with nalidixic acid MYPGPnp MYPGP agar enriched with nalidixic acid and pipemidic acid

20 METRICULT Sample collection kit for endometritis examination in dairy cows.

• Guarantees uncontaminated sampling • Also suitable for examination of mares • Does not affect fertility

The sampling kit is assembled from: A disposable catheter for the introduction of a swab used to collect a microbiological sample from the uterus of a dairy cow, without the risk of microbiological contamination from the vagina environment of the animal being examined

A tube with Amies transport medium for transport of the sample to the laboratory for microbiological examination During the verification of the cleanliness of the collected sample at the SVI Jihlava it was con- firmed that the collected sample was indeed free of contamination from the cow‘s vagina environ- ment. These results were important for the regis- tration of the product of LabMediaServis s.r.o. as a “Veterinary-technical­ device” at ISCVBM and reg- istration as a collection kit as patent No. 305628.

The catheter tip (tampon cover) is made of biodegradable material.

60 cm

For more information see www.labmediaservis.cz 21 MICROMAST MicroMast is a cultivation kit for rapid on-farm diagnosis of mastitis pathogens.

Benefits: • Simplicity in design. • Reduced antibiotic consumption. • Increased treatment efficiency, lower culling rates. • More milk in a delivery. • Rapid pathogen eradication from the herd. • Use of results in herd management. • Preservation of the culture for antimicrobial susceptibility determination. • Drying-off without antibiotics.

Expert advice: MVDr. Josef Prášek, Ph.D. +420 775 956 986 [email protected] www.josefprasek.cz

Type of packaging: Test tube, sterile loop, disinfectant wipe, a three-compartment Petri dish ∅ 90 mm with three different broths. A B Broth ”A“ is for the detection of G- bacteria. C Broth ”B“ has General purpose; it allows detection of both G- and G+ bacteria. Broth ”C“ is for the detection of G+ bacteria.

22 PM TEST (Pure milk test) PM test allows rapid diagnosis up to the species level Examples of microorganism species specifically of mastitis pathogens. growing on PM test: Compartment G- Compartment STAPH: PM test allows: Escherichia coli Staphylococcus aureus • Determination of mastitis pathogen species. Klebsiella oxytoca Staphylococcus chromogenes • Minimization of antibiotic consumption. Enterobacter spp. Staphylococcus epidermidis • Targeted use of antibiotics restricted for specific indications. Serratia marcescens Staphylococcus haemolyticus • Effective work and cost savings within the anti-mastitis Pseudomonas aeruginosa Staphylococcus sciuri programme and differentiated drying off in dairy cows. Proteus vulgaris Staphylococcus xylosus Candida rugosa Staphylococcus saprophyticus PM test advantages: Micrococcus luteus • Commercially available, fast and inexpensive. Compartment STREP: Bacillus spp. • On-farm diagnosis of mastitis pathogen. Streptococus agalactiae • Simple reading of results based on different colours of colonies. Streptococcus dysgalactiae Streptococcus uberis • Rapid screening for shedding contagious or environmental Streptococcus parauberis pathogens in dairy cows within anti-mastitis programmes. Enterococcus faecalis Aerococcus viridans spp.

Expert advice: MVDr. Miroslav Věříš +420 603 218 079 [email protected]

Type of packaging: Test tube, sterile loop, disinfectant wipe, three-compartment Petri dish ∅ 90 mm with three different chromogenic broths: Compartment ”Staph“ for the detection of staphylococci. Compartment ”Strep“ for the detection of streptococci. Compartment ”G-“ for the detection of gram-negative bacteria (in G- compartment, detection of yeasts and fungi is possible).

For more information see www.labmediaservis.cz 23 PET TEST Kit for rapid diagnosis of infectious agents in small animals.

PET test allows: Compartments • Diagnostics of causative agents of intestinal and urinary infections. The original chromogenic agars allow only one group • Diagnostics of infections in swabs (ear, eye, vagina). of infectious agents to grow, while the other bacteria • Diagnostics of pathogens from swabs in wounds, skin and other are inhibited. biological materials. • If needed, ATB sensitivity can be determined from the grown cultures. G- rods: • Unambiguous and clear reading of disease agents in small animals. E. coli, Pasteurella multocida, Yersinia enterocolitica, • Bacteria growing on chromogenic media are typically stained, allowing Salmonella enteritidis, Morganella morganii, Proteus direct reading of disease agents from the Petri dish. mirabilis... + yeasts in the genus Candida

Advantages of PET test: Streptococci: • Commercially available and rapid test for clinical veterinary practice. Streptococcus spp. (agalactiae, canis...) • Enables targeted ATB treatment. • Contributes to preventing the emergence of multi-resistant strains. Staphylococci: • Easy way to high quality results. Staphylococcus spp. (aureus, epidermidis, intermedius...) • Unique product on the market.

The result is available after 24-h incubation.

24 FOOD AND WATER HYGIENE – LIQUID MEDIA

Recommen- Product Description Purpose Category Packaging dation BB Bolton broth Selective isolation and enrichment of Campylobacter spp. Selective TT, V FR Saline solution Dilution of clinical and other samples Diluent TT, V FRB Fraser broth Selective isolation an enrichment Listeria spp. Selective TT, V FRB1/2 Fraser broth 1/2 Selective isolation and enrichment of Listeria spp. Selective TT, V Peptone FRPP Dilution of clinical and other samples Diluent TT, V saline solution Detection and enumeration GLCT Giolitti-Cantoni broth Selective TT, V of coagulase-positive staphylococci in samples LAK Lactobroth Detection of coliform bacteria Diagnostic TT, V Selective isolation and enumeration of Clostridium LS Lactose – Sulfite broth Selective TT, V perfringens Müller-Kauffman MKTTN tetrathionate- Selective isolation of Salmonella spp. Selective TT, V novobiocin broth Modified lauryl MLST Selective enumeration of Enterobacter sakazakii Selective TT, V sulfate tryptose broth MOSEL Mossel EE broth Selective enumeration of Enterobacteriaceae Selective TT, V Lactose PEPLAK Detection of coliform bacteria Diagnostic TT, V Peptone Broth Rappaport RVS Vassiliadis Soya Isolation of Salmonellae Selective TT, V Broth (RVS broth)

TT = Test Tube; V = Vial Food Water For more information see www.labmediaservis.cz 25 PCA, VRBG ALOA ESIA Listeria monocytogenes Enterobacter (Cronobacter) sakazakii

FOOD AND WATER HYGIENE – SOLID MEDIA

Recommen- Product Description Purpose Category Packaging dation Listeria Chromogenic Isolation, preliminary identification and enumeration Selective- ALOA PD90 agar (ALOA) of Listeria spp. and Listeria monocytogenes in samples diagnostic Selective isolation and preliminary identification Selective- BEA Bile esculin azide agar PD90, TT of group D streptococci diagnostic Selective isolation, identification and enumeration Selective- BP Baird-Parker agar PD90 of Staphylococus aureus in samples diagnostic Chromogenic Coliform Simultaneous detection of coliform bacteria Selective- CHCLT PD90, V agar and Escherichia coli diagnostic Selective isolation, and enumeration of Yersinia CIN CIN – Yersinia agar Selective PD90 enterocolitica in samples Identification and enumeration of Pseudomonas CN Pseudomonas agar Selective PD90 aeruginosa in samples by membrane filter technique Clostridium Preliminary identification and enumeration CP Selective PD90 perfringens agar of Clostridium perfingens in samples DRBC agar with dich- Selective isolation and enumeration of yeasts DRBC loran, Bengal red and Selective PD90 and fungi in samples chloramphenicol Isolation and preliminary identification of bacteria Selective- PD90, ENDO ENDO agar in the family Enterobacteriaceae diagnostic PD55 Chromogenic Entero- Selective isolation, differentiation and enumera- Selective- ESIA PD90 bacter sakazakii agar tion of Enterobacter sakazakii in samples diagnostic Selective isolation and cultivation of bacteria LEGV Legionella GVPC agar Selective PD90 of the genus Legionella Selective isolation and enumeration of bacteria MC MacConkey agar Selective PD90 of the Enterobacteriaceae family in samples Campylobacter blood- Selective isolation and cultivation of Campy- MCCDA free selective medium lobacter spp., especially C. jejuni, C. coli and Selective PD90 (modified CCDA - Preston) C. laridis Selective isolation and enumeration of Clostridi- MCP M-CP agar um perfringens in samples using membrane filter Selective PD55 technique

26 = Chromogenic media; V = Vial Food Water PCA XLD XLD Staphylococcus aureus Escherichia coli Salmonella typhimurium

FOOD AND WATER HYGIENE – SOLID MEDIA

Recommen- Product Description Purpose Category Packaging dation Sorbitol Selective isolation and detection MCS selektivní PD90 MacConkey agar of Escherichia coli O157:H7 Detection and enumeration of coliforms M-FC M-FC agar selektivní PD90, V in samples by membrane filter technique Isolation, cultivation and enumeration of bacteria MRS MRS agar selektivní PD90, V of the species Lactobacillus in samples Selective isolation and detection selektivně- PALCAM Palcam Listeria agar PD90 of Listeria monocytogenes diagnostická Plate count agar Isolation, cultivation and enumeration of total PD90, PCA obecná (PCA / GTK) number of microorganisms in samples PCA, V Isolation and cultivation of yeasts, fungi and PD90, SAB Sabouraud agar obecná acidophilic bacteria TT, V Isolation, detection and enumeration of enterococci PD90, SBA Slanetz-Bartley agar selektivní in samples by membrane filter technique PD55, V Selective isolation and cultivation of bacteria TCBS TCBS agar selektivní PD90 of the genus Vibrio Cultivation of a wide range of microorganisms PD90, TSA Tryptic-soy agar obecná and environment sterility testing PCA, V Selective isolation, detection and enumeration of PD90, VRBG VRBG agar selektivní bacteria in the family Enterobacteriaceae in samples PCA, V Selective isolation, detection and enumeration VRBL VRBL agar selektivní PD90, V of coliform bacteria in samples YGC YGC (GKCH) agar Selective isolation and enumeration of fungi in samples selektivní PD90, V Xylose Lysine Selective isolation and cultivation of enterobacteria and selektivně- XLD PD90 Deoxycholate agar preliminary identification of Salmonella and Shigella diagnostická

PD90 = Petri Dish ∅ 90 mm; PD55 = Petri Dish ∅ 55 mm; PCA = Plate Count Agar For more information see www.labmediaservis.cz 27 LabMediaServis s.r.o. Národní 84 551 01 Jaroměř +420 491 810 091 Graphic design: Manufacturer of culture [email protected] Pavla Balcarová media and blood derivatives www.labmediaservis.cz [email protected]