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Bladder Cancer Associated Protein) Wu Xinxing, Zhao Min, Zuo Zehua School of Medicine, Wuhan University, Wuhan, Hubei, 430071, P.R

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Bladder Cancer Associated Protein) Wu Xinxing, Zhao Min, Zuo Zehua School of Medicine, Wuhan University, Wuhan, Hubei, 430071, P.R Atlas of Genetics and Cytogenetics in Oncology and Haematology OPEN ACCESS JOURNAL AT INIST-CNRS Gene Section Mini Review BLCAP (bladder cancer associated protein) Wu Xinxing, Zhao Min, Zuo Zehua School of Medicine, Wuhan University, Wuhan, Hubei, 430071, P.R. China (WX, ZM, ZZ) Published in Atlas Database: May 2008 Online updated version : http://AtlasGeneticsOncology.org/Genes/BLCAPID801ch20q11.html DOI: 10.4267/2042/44444 This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 2.0 France Licence. © 2009 Atlas of Genetics and Cytogenetics in Oncology and Haematology Identity Function BLCAP protein was a member of transmembrane Other names : BC10 protein 1. By analyzing the signal peptide of BLCAP HGNC (Hugo): BLCAP protein, we found a cleavage site in 63-64 aa. It showed Location : 20q11.23 that there might be three phosphorylated sites: 68 aa, 73 Note : Bladder cancer associated protein is a protein aa and 78 aa. In 78-81 aa, a typical [ST]-X(2)-[DE] which is believed to be involved in the carcinogenesis. structure- the phosphorylated site of tyrosine protein Lower levels of BLCAP have been identified in kinase was found. advanced bladder cancer and cervical carcinoma, suggesting a correlation between lower-expression of Homology BLCAP and tumor development. The comparability between the sequences of BLCAP gene and Homo sapiens mRNA (DKFZP564M053) or DNA/RNA BC10 was 99% and 87% respectively. Description Implicated in The BLCAP gene located in chromosome 20q11.2-q12, Cervical cancer consists of only one exon and the coding region spans 264 bp. The comparability between the sequences of Disease BLCAP gene and Homo sapiens mRNA The bladder cancer-associated protein gene (BLCAP) (DKFZP564M053) or BC10 was 99% and 87% was among the differentially expressed genes in respectively. cervical cancer. BLCAP is expressed highly in non cancerous cervical tissues, but it is greatly lost in primary cervical cancer tissue. After transfected Protein BLCAP cDNA into HeLa cells, HeLa cells shows reduced cell growth and clone genicity compared to the Description vector-transfected cognate cells. BLCAP expression in The protein encoded by BLCAP were composite of HeLa cells leads to growth arrest and significantly Leu(19.5%), Pro(9.19%), ser(8.04%), cys(8.04%) and enhanced apoptosis in vitro and reduced tumor other amino acids. The N-terminal of the protein which formation in vivo. was encoded by BLCAP gene was an alpha helix. At Bladder cancer the C-terminal, it was beta sheet and in the middle, it was coil. The two terminal regions were more Disease hydrophobile than the middle region. Between 45-65 The bladder cancer-associated protein gene (BLCAP, aa, there was a transmembrane region. bladder cancer, Mr 10 kDa) was exclusively expressed in the non invasive lesions of bladder cancer. The full- Expression length bc10 cDNA contains a complete open reading Expression in human tissues with a relatively low level frame (ORF) of 263 bp and encodes a protein of expression in cervix, bladder, testis, placenta, spleen composed of 87 amino acids that has no homology to and peripheral blood leukocytes. any of the known protein families. Transient expression Atlas Genet Cytogenet Oncol Haematol. 2009; 13(4) 258 BLCAP (bladder cancer associated protein) Xinxing W, et al. of bc10 cDNA in COS1 cells yielded a primary Osteosarcoma translation product with an apparent Mr of 9.8 kDa and Disease pI of 6.7, in agreement with the theoretical calculated The positive rate of BLCAP protein in primary value. Comparison of mouse and human bc10 genomic osteosarcoma was very high, much higher than that in loci revealed an intron less organization of the coding recurrent osteosarcoma. It may be helpful for region in both species as well as a highly conserved evaluating the prognosis of osteosarcoma to study the structure having 91% and 100% identity at the DNA relationship between expression of BLCAP protein and (coding region) and protein levels, respectively. malignancy of osteosarcoma. Southern analysis did not reveal gross DNA rearrangements within the bc10 genomic locus in the invasive bladder tumors, implying that the differential References expression of the gene most likely reflects alterations in Gromova I, Gromov P, Celis JE. bc10: A novel human bladder messenger expression (transcription and/or mRNA cancer-associated protein with a conserved genomic structure decay). The downregulation of this novel marker in downregulated in invasive cancer. Int J Cancer. 2002 Apr 1;98(4):539-46 invasive tumors suggests a putative role in bladder cancer progression. Su HC, Zhao YH, Fan DG, Fan QY, Zhang P, Wen YH, Liu YY. [Relationship between expression of BLCAP protein and Tongue carcinoma malignancy of osteosarcoma]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2003 Sep;19(5):465-6 Disease Bladder cancer-associated protein gene (BLCAP) is a Clutterbuck DR, Leroy A, O'Connell MA, Semple CA. A bioinformatic screen for novel A-I RNA editing sites reveals novel candidate tumor suppressor gene identified from recoding editing in BC10. Bioinformatics. 2005 Jun the human bladder carcinoma. BLCAP overexpression 1;21(11):2590-5 could inhibit cell growth by inducing apoptosis in HeLa Evans HK, Weidman JR, Cowley DO, Jirtle RL. Comparative cells. Such evidence suggests the alterations in BLCAP phylogenetic analysis of blcap/nnat reveals eutherian-specific may play an important role in tumorigenesis. imprinted gene. Mol Biol Evol. 2005 Aug;22(8):1740-8 Overexpressed BLCAP resulted in growth inhibition of Zuo Z, Zhao M, Liu J, Gao G, Wu X. Functional analysis of the human tongue cancer cell line Tca8113 in vitro, bladder cancer-related protein gene: a putative cervical cancer accompanied by S phase cell cycle arrest and apoptosis. tumor suppressor gene in cervical carcinoma. Tumour Biol. The growth inhibition was correlated with up- 2006;27(4):221-6 regulation of p21 (WAF1/CIP1) expression and down- Zuo ZH, Zhao M, Liu J, Wei Y, Wu XX. [Inhibitory effect of regulation of Bcl-XL and Bcl-2 expressions. However, bladder cancer related protein gene on HeLa cell proliferation]. p53 expression and NF-kappaB activity remained Ai Zheng. 2006 Jul;25(7):811-7 unchanged post infection. Furthermore, no changes in Yao J, Duan L, Fan M, Yuan J, Wu X. Overexpression of p53 phosphorylation at Ser46 and nuclear localization, BLCAP induces S phase arrest and apoptosis independent of p53 and NF-kappaB in human tongue carcinoma : BLCAP which are critical to p53 function, were observed in overexpression induces S phase arrest and apoptosis. Mol Cell BLCAP-overexpressed cells. Taken together, BLCAP Biochem. 2007 Mar;297(1-2):81-92 may Nishimoto Y, Yamashita T, Hideyama T, Tsuji S, Suzuki N, play a role not only in regulating cell proliferation but Kwak S. Determination of editors at the novel A-to-I editing also in coordinating apoptosis and cell cycle via a novel positions. Neurosci Res. 2008 Jun;61(2):201-6 way independent of p53 and NF-kappaB. This article should be referenced as such: Xinxing W, Min Z, Zehua Z. BLCAP (bladder cancer associated protein). Atlas Genet Cytogenet Oncol Haematol. 2009; 13(4):258-259. Atlas Genet Cytogenet Oncol Haematol. 2009; 13(4) 259.
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