Product data sheet MedKoo Cat#: 326944 Name: CAS#: 53-43-0 Chemical Formula: C19H28O2 Exact Mass: 288.2089 Molecular Weight: 288.431 Product supplied as: Powder Purity (by HPLC): ≥ 98% Shipping conditions Ambient temperature Storage conditions: Powder: -20°C 3 years; 4°C 2 years. In solvent: -80°C 3 months; -20°C 2 weeks.

1. Product description: Dehydroepiandrosterone, also known as trans-Dehydroandrosterone, DHEA, more correctly didehydroepiandrosterone; androstenolone and 3β-hydroxyandrost-5-en-17-one or 5-androsten-3β-ol-17-one, is an endogenous hormone. It is the most abundant circulating in humans, in whom it is produced in the adrenal glands, the gonads, and the brain, where it functions predominantly as a metabolic intermediate in the biosynthesis of the and sex . However, DHEA also has a variety of potential biological effects in its own right, binding to an array of nuclear and cell surface receptors, and acting as a .

2. CoA, QC data, SDS, and handling instruction SDS and handling instruction, CoA with copies of QC data (NMR, HPLC and MS analytical spectra) can be downloaded from the product web page under “QC And Documents” section. Note: copies of analytical spectra may not be available if the product is being supplied by MedKoo partners. Whether the product was made by MedKoo or provided by its partners, the quality is 100% guaranteed.

3. Solubility data Solvent Max Conc. mg/mL Max Conc. mM DMSO 53.5 185.49 DMF 25.0 86.68 Ethanol 53.5 185.49 DMSO:PBS (pH 7.2) 0.5 1.73 (1:1)

4. Stock solution preparation table: Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg 1 mM 3.47 mL 17.34 mL 34.67 mL 5 mM 0.69 mL 3.47 mL 6.93 mL 10 mM 0.35 mL 1.73 mL 3.47 mL 50 mM 0.07 mL 0.35 mL 0.69 mL

5. Molarity Calculator, Reconstitution Calculator, Dilution Calculator Please refer the product web page under section of “Calculator”

6. Recommended literature which reported protocols for in vitro and in vivo study In vitro study 1. Joshi K, Hassan SS, Ramaraj P. Differential biological effects of dehydroepiandrosterone (DHEA) between mouse (B16F10) and human melanoma (BLM) cell lines. Dermatoendocrinol. 2017 Nov 20;9(1):e1389360. doi: 10.1080/19381980.2017.1389360. PMID: 29484102; PMCID: PMC5821161. 2. Ding X, Yu L, Ge C, Ma H. Protective effect of DHEA on hydrogen peroxide-induced oxidative damage and apoptosis in primary rat Leydig cells. Oncotarget. 2017 Mar 7;8(10):16158-16169. doi: 10.18632/oncotarget.15300. PMID: 28212544; PMCID: PMC5369954.

In vivo study

MedKoo Biosciences || http://www.medkoo.com || [email protected] 2500 Gateway Centre Blvd Suite 400, Morrisville, NC27560, USA. Tel: 919-636-5577, Fax: 919-980-4831

Product data sheet 1. Tao T, Liu GJ, Shi X, Zhou Y, Lu Y, Gao YY, Zhang XS, Wang H, Wu LY, Chen CL, Zhuang Z, Li W, Hang CH. DHEA Attenuates Microglial Activation via Induction of JMJD3 in Experimental Subarachnoid Haemorrhage. J Neuroinflammation. 2019 Nov 28;16(1):243. doi: 10.1186/s12974-019-1641-y. PMID: 31779639; PMCID: PMC6883548. 2. Qiu X, Gui Y, Xu Y, Li D, Wang L. DHEA promotes osteoblast differentiation by regulating the expression of osteoblast-related genes and Foxp3(+) regulatory T cells. Biosci Trends. 2015 Oct;9(5):307-14. doi: 10.5582/bst.2015.01073. PMID: 26559023.

7. Bioactivity Biological target: DHEA () mediates its action via multiple signaling pathways involving specific membrane receptors and via transformation into androgen and estrogen derivatives acting through their specific receptors.

In vitro activity ROS, ·OH and MDA contents were significantly increased in H2O2-treated group when compared to control group (P < 0.05) (Figure 2). Pre-treatment with 10μM DHEA reduced intracellular ROS levels relative to that in H2O2-treated group (P < 0.05) (Figure 2A). Pre-treatment with 1-100μM DHEA significantly decreased ·OH content (P < 0.01) (Figure 2B), while no significant differences were observed on the O2- content (Figure 2C). Compared to H2O2-treated group, MAD contents were decreased in the cells pre-treated with 10 and100μM DHEA (P < 0.05) (Figure 2D).

Reference: Oncotarget. 2017 Mar 7; 8(10): 16158–16169. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5369954/

In vivo activity Mice pretreated with DHEA had remarkably lower TUNEL+ NeuN+ proportion than those in the SAH (subarachnoid haemorrhage) group (n = 3, 54.24 ± 2.24% in the SAH group versus 29.04 ± 6.66% in the SAH + DHEA group). The water content in the ipsilateral hemisphere was 80.63 ± 0.75% in the SAH + DHEA group, which was lower but not significantly than the 83.30 ± 0.81% observed in the SAH group (n = 6). Furthermore, DHEA improved sensorimotor functions both at POD 1 and POD 7. These results indicate that DHEA plays a neuroprotective role in SAH mice.

Reference: J Neuroinflammation. 2019; 16: 243. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6883548/

Note: The information listed here was extracted from literature. MedKoo has not independently retested and confirmed the accuracy of these methods. Customer should use it just for a reference only.

MedKoo Biosciences || http://www.medkoo.com || [email protected] 2500 Gateway Centre Blvd Suite 400, Morrisville, NC27560, USA. Tel: 919-636-5577, Fax: 919-980-4831