Role of Sulfiredoxin Interacting Proteins in Lung Cancer Development

Total Page:16

File Type:pdf, Size:1020Kb

Role of Sulfiredoxin Interacting Proteins in Lung Cancer Development University of Kentucky UKnowledge Theses and Dissertations--Toxicology and Cancer Biology Toxicology and Cancer Biology 2016 ROLE OF SULFIREDOXIN INTERACTING PROTEINS IN LUNG CANCER DEVELOPMENT Hedy Chawsheen University of Kentucky, [email protected] Digital Object Identifier: http://dx.doi.org/10.13023/ETD.2016.176 Right click to open a feedback form in a new tab to let us know how this document benefits ou.y Recommended Citation Chawsheen, Hedy, "ROLE OF SULFIREDOXIN INTERACTING PROTEINS IN LUNG CANCER DEVELOPMENT" (2016). Theses and Dissertations--Toxicology and Cancer Biology. 13. https://uknowledge.uky.edu/toxicology_etds/13 This Doctoral Dissertation is brought to you for free and open access by the Toxicology and Cancer Biology at UKnowledge. It has been accepted for inclusion in Theses and Dissertations--Toxicology and Cancer Biology by an authorized administrator of UKnowledge. For more information, please contact [email protected]. STUDENT AGREEMENT: I represent that my thesis or dissertation and abstract are my original work. Proper attribution has been given to all outside sources. I understand that I am solely responsible for obtaining any needed copyright permissions. I have obtained needed written permission statement(s) from the owner(s) of each third-party copyrighted matter to be included in my work, allowing electronic distribution (if such use is not permitted by the fair use doctrine) which will be submitted to UKnowledge as Additional File. I hereby grant to The University of Kentucky and its agents the irrevocable, non-exclusive, and royalty-free license to archive and make accessible my work in whole or in part in all forms of media, now or hereafter known. I agree that the document mentioned above may be made available immediately for worldwide access unless an embargo applies. I retain all other ownership rights to the copyright of my work. I also retain the right to use in future works (such as articles or books) all or part of my work. I understand that I am free to register the copyright to my work. REVIEW, APPROVAL AND ACCEPTANCE The document mentioned above has been reviewed and accepted by the student’s advisor, on behalf of the advisory committee, and by the Director of Graduate Studies (DGS), on behalf of the program; we verify that this is the final, approved version of the student’s thesis including all changes required by the advisory committee. The undersigned agree to abide by the statements above. Hedy Chawsheen, Student Dr. Qiou Wei, Major Professor Dr. Isabel Mellon, Director of Graduate Studies ROLE OF SULFIREDOXIN INTERACTING PROTEINS IN LUNG CANCER DEVELOPMENT DISSERTATION A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the College of Medicine at the University of Kentucky By Hedy Ahmed Chawsheen Lexington, Kentucky Co-Directors: Dr. Qiou Wei, Assistant Professor of Toxicology and Cancer Biology and : Dr. Daret St. Clair, Professor of Toxicology and Cancer Biology Lexington, Kentucky Copyright © Hedy Chawsheen 2016 ABSTRACT OF DISSERTATION ROLE OF SULFIREDOXIN INTERACTING PROTEINS IN LUNG CANCER DEVELOPMENT Sulfiredoxin (Srx) is an antioxidant enzyme that can be induced by oxidative stress. It promotes oncogenic phenotypes of cell proliferation, colony formation, migration, and metastasis in lung, skin and colon cancers. Srx reduces the overoxidation of 2-cysteine peroxiredoxins in cells, in addition to its role of removing glutathione modification from several proteins. In this study, I explored additional physiological functions of Srx in lung cancer through studying its interacting proteins. Protein disulfide isomerase (PDI) family members, thioredoxin domain containing protein 5 (TXNDC5) and protein disulfide isomerase family A member 6 (PDIA6), were detected to interact with Srx. Therefore, I proposed that TXNDC5 and PDIA6 are important for the oncogenic phenotypes of Srx in lung cancer. In chapter one, I presented background information about the role of Srx as an antioxidant enzyme in cancer. I also explained the functional significance of PDIs as oxidoreductase and chaperones in cells. In chapter two, I verified the Srx- TXNDC5/PDIA6 interaction in HEK293T and A549 cells by co-immunoprecipitation and other assays. In TXNDC5 and PDIA6, the N-terminal thioredoxin-like domain (D1) is determined to be the main platform for interaction with Srx. The Srx-TXNDC5 interaction was enhanced by H2O2 treatment in A549 cells. Srx was determined to localize in the endoplasmic reticulum (ER) of A549 cells along with TXNDC5 and PDIA6. This localization was confirmed by both subcellular fractionation and immunofluorescence imaging experiments. In chapter three I focused on studying the physiological function of Srx interacting proteins in the ER. A549 subcellular fractionation results showed that TXNDC5 facilitates Srx retention in the ER. Moreover, TXNDC5 and Srx were found to participate in chaperone activities in lung cancer. Both proteins contributed in the refolding of heat-shock induced protein aggregates. In addition, TXNDC5 and PDIA6 were found to enhance the protein refolding in response to H2O2 treatment. Conversely, Srx appeared to have an inhibitory effect on protein folding under same treatment conditions. Downregulation of Srx, TXNDC5, or PDIA6 significantly reduced cell viability in response to tunicamycin treatment. TXNDC5 knockdown decreased the time required for the splicing of X-box binding protein-1 (XBP-1). In either knockdown Srx or TXNDC5 cells, there was an observable decrease in the expression of GRP78 and the splicing of spliced XBP-1. These results suggest a possible role of Srx in unfolded protein response signaling. TXNDC5 and PDIA6, similar to Srx, contribute to the proliferation, anchorage independent colony formation and migration of lung cancer cells. In this dissertation I concluded that Srx TXNDC5, and PDIA6 proteins participate in oxidative protein folding in lung cancer. Srx and TXNDC5 can modulate unfolded protein response (UPR) sensor activation and growth inhibition. Furthermore, TXNDC5 and PDIA6 can promote tumorigenesis of lung cancer cells. Therefore, the molecular interaction of Srx with TXNDC5/PDIA6 has the potential to be used as novel therapeutic targets for lung cancer treatment. KEYWORDS: Sulfiredoxin, thioredoxin domain containing protein 5, protein disulfide isomerase A isoform 6, interaction, function Hedy Ahmed Chawsheen April 26, 2016 ROLE OF SULFIREDOXIN INTERACTING PROTEINS IN LUNG CANCER DEVELOPMENT By Hedy Ahmed Chawsheen Qiou Wei, Ph.D. Co-Director of Dissertation Daret St. Clair, Ph.D. Co-Director of Dissertation Isabel Mellon, Ph.D. Director of Graduate Studies April 26, 2016 I dedicate this dissertation to my family ACKNOWLEDGMENTS I want to thank my advisor, Dr. Qiou Wei, for providing me the opportunity to work in his lab. I am grateful for all his contribution of time, guidance and support, to make my research experience productive and inspiring. I am privileged to have Dr. Daret St. Clair, Dr. Tadahide Izumi, Dr. Hsin-Sheng Yang, and Dr. Jia Luo as members in my committee, with much of appreciation for their valuable feedbacks on my research. I would also like to thank Dr. Edmond Rucker, who is the external examiner of the final exam. I would like to acknowledge the Human Capacity Development Program of Ministry of Higher Education and Scientific Research in Kurdistan Regional Government-Iraq for their financial sponsorship during my coursework at the University. I would also like to express my gratitude to Dr. Hong Jiang for her patience and generosity in providing technical assistance when I needed. Thanks also go to my colleague, Murli Mishra, for his help and cooperation in the lab. This work would not be possible without the inspiration from my parents, Ahmed Chawsheen and Sabria Mustafa, and my brothers and sisters. Much of appreciation goes to my husband, Zana R. Majeed, for his constant encouragement and support throughout my study period. iii TABLE OF CONTENTS Acknowledgments ............................................................................................................ iii Table of Contents ............................................................................................................. iv List of Figures ................................................................................................................... vi Chapter One: Introduction 1.1. Background ............................................................................................................ 1 Srx in Cancer ............................................................................................................. 3 The PDI Family .......................................................................................................... 5 TXNDC5 and PDIA6 in Cancer ................................................................................ 15 Research Objectives ................................................................................................ 20 1.2. Materials and Methods ......................................................................................... 21 Cell Lines and Chemicals ........................................................................................ 21 Plasmid Constructs and Lentiviral Production ......................................................... 21 Site Directed Mutagenesis ....................................................................................... 24 Protein Purification ..................................................................................................
Recommended publications
  • Environmental Influences on Endothelial Gene Expression
    ENDOTHELIAL CELL GENE EXPRESSION John Matthew Jeff Herbert Supervisors: Prof. Roy Bicknell and Dr. Victoria Heath PhD thesis University of Birmingham August 2012 University of Birmingham Research Archive e-theses repository This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder. ABSTRACT Tumour angiogenesis is a vital process in the pathology of tumour development and metastasis. Targeting markers of tumour endothelium provide a means of targeted destruction of a tumours oxygen and nutrient supply via destruction of tumour vasculature, which in turn ultimately leads to beneficial consequences to patients. Although current anti -angiogenic and vascular targeting strategies help patients, more potently in combination with chemo therapy, there is still a need for more tumour endothelial marker discoveries as current treatments have cardiovascular and other side effects. For the first time, the analyses of in-vivo biotinylation of an embryonic system is performed to obtain putative vascular targets. Also for the first time, deep sequencing is applied to freshly isolated tumour and normal endothelial cells from lung, colon and bladder tissues for the identification of pan-vascular-targets. Integration of the proteomic, deep sequencing, public cDNA libraries and microarrays, delivers 5,892 putative vascular targets to the science community.
    [Show full text]
  • A Computational Approach for Defining a Signature of Β-Cell Golgi Stress in Diabetes Mellitus
    Page 1 of 781 Diabetes A Computational Approach for Defining a Signature of β-Cell Golgi Stress in Diabetes Mellitus Robert N. Bone1,6,7, Olufunmilola Oyebamiji2, Sayali Talware2, Sharmila Selvaraj2, Preethi Krishnan3,6, Farooq Syed1,6,7, Huanmei Wu2, Carmella Evans-Molina 1,3,4,5,6,7,8* Departments of 1Pediatrics, 3Medicine, 4Anatomy, Cell Biology & Physiology, 5Biochemistry & Molecular Biology, the 6Center for Diabetes & Metabolic Diseases, and the 7Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202; 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, Indianapolis, IN, 46202; 8Roudebush VA Medical Center, Indianapolis, IN 46202. *Corresponding Author(s): Carmella Evans-Molina, MD, PhD ([email protected]) Indiana University School of Medicine, 635 Barnhill Drive, MS 2031A, Indianapolis, IN 46202, Telephone: (317) 274-4145, Fax (317) 274-4107 Running Title: Golgi Stress Response in Diabetes Word Count: 4358 Number of Figures: 6 Keywords: Golgi apparatus stress, Islets, β cell, Type 1 diabetes, Type 2 diabetes 1 Diabetes Publish Ahead of Print, published online August 20, 2020 Diabetes Page 2 of 781 ABSTRACT The Golgi apparatus (GA) is an important site of insulin processing and granule maturation, but whether GA organelle dysfunction and GA stress are present in the diabetic β-cell has not been tested. We utilized an informatics-based approach to develop a transcriptional signature of β-cell GA stress using existing RNA sequencing and microarray datasets generated using human islets from donors with diabetes and islets where type 1(T1D) and type 2 diabetes (T2D) had been modeled ex vivo. To narrow our results to GA-specific genes, we applied a filter set of 1,030 genes accepted as GA associated.
    [Show full text]
  • Supplementary Materials
    1 Supplementary Materials: Supplemental Figure 1. Gene expression profiles of kidneys in the Fcgr2b-/- and Fcgr2b-/-. Stinggt/gt mice. (A) A heat map of microarray data show the genes that significantly changed up to 2 fold compared between Fcgr2b-/- and Fcgr2b-/-. Stinggt/gt mice (N=4 mice per group; p<0.05). Data show in log2 (sample/wild-type). 2 Supplemental Figure 2. Sting signaling is essential for immuno-phenotypes of the Fcgr2b-/-lupus mice. (A-C) Flow cytometry analysis of splenocytes isolated from wild-type, Fcgr2b-/- and Fcgr2b-/-. Stinggt/gt mice at the age of 6-7 months (N= 13-14 per group). Data shown in the percentage of (A) CD4+ ICOS+ cells, (B) B220+ I-Ab+ cells and (C) CD138+ cells. Data show as mean ± SEM (*p < 0.05, **p<0.01 and ***p<0.001). 3 Supplemental Figure 3. Phenotypes of Sting activated dendritic cells. (A) Representative of western blot analysis from immunoprecipitation with Sting of Fcgr2b-/- mice (N= 4). The band was shown in STING protein of activated BMDC with DMXAA at 0, 3 and 6 hr. and phosphorylation of STING at Ser357. (B) Mass spectra of phosphorylation of STING at Ser357 of activated BMDC from Fcgr2b-/- mice after stimulated with DMXAA for 3 hour and followed by immunoprecipitation with STING. (C) Sting-activated BMDC were co-cultured with LYN inhibitor PP2 and analyzed by flow cytometry, which showed the mean fluorescence intensity (MFI) of IAb expressing DC (N = 3 mice per group). 4 Supplemental Table 1. Lists of up and down of regulated proteins Accession No.
    [Show full text]
  • Sodium Butyrate Improves Antioxidant Stability in Sub-Acute Ruminal
    Ma et al. BMC Veterinary Research (2018) 14:275 https://doi.org/10.1186/s12917-018-1591-0 RESEARCH ARTICLE Open Access Sodium butyrate improves antioxidant stability in sub-acute ruminal acidosis in dairy goats Nana Ma†, Juma Ahamed Abaker†, Muhammad Shahid Bilal, Hongyu Dai and Xiangzhen Shen* Abstract Background: Currently, little is known about the effect of sodium butyrate (NaB) on oxidative stress following grain-induced sub-acute ruminal acidosis in dairy goats. In the present study, 18 lactating dairy goats implanted with a ruminal cannula and permanent indwelling catheters in the portal and hepatic veins were randomly allocated into 3 treatment groups over 20 weeks: low grain (LG, 40% grain; n = 6), high grain (HG, 60% grain; n =6) and high grain with sodium butyrate (HG + NaB, 60% grain + NaB; n = 6). Results: When added to the HG diet, NaB increased the mean ruminal pH and reduced the levels of ruminal, portal and hepatic LPS; Additionally, we observed an increase in SOD1, SOD2, SOD3, GPX1 and CAT mRNA expression, increased levels of TSOD and CAT enzyme activity as well as increased total antioxidant capacity (T-AOC) and decreased malondialdehyde (MDA) in both the liver and plasma, while GPx activity increased in the liver of goats fed the HG + NaB diet. The mRNA expression of UGT1A1, NQO1, MGST3, and Nrf2, as well as total Nrf2 protein levels were increased in goats fed the HG + NaB diet. Conclusions: Our study indicates that sodium butyrate could improve the oxidative status in sub-acute ruminal acidosis through the partial activation of Nrf2-dependent genes.
    [Show full text]
  • Preclinical Evaluation of Protein Disulfide Isomerase Inhibitors for the Treatment of Glioblastoma by Andrea Shergalis
    Preclinical Evaluation of Protein Disulfide Isomerase Inhibitors for the Treatment of Glioblastoma By Andrea Shergalis A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy (Medicinal Chemistry) in the University of Michigan 2020 Doctoral Committee: Professor Nouri Neamati, Chair Professor George A. Garcia Professor Peter J. H. Scott Professor Shaomeng Wang Andrea G. Shergalis [email protected] ORCID 0000-0002-1155-1583 © Andrea Shergalis 2020 All Rights Reserved ACKNOWLEDGEMENTS So many people have been involved in bringing this project to life and making this dissertation possible. First, I want to thank my advisor, Prof. Nouri Neamati, for his guidance, encouragement, and patience. Prof. Neamati instilled an enthusiasm in me for science and drug discovery, while allowing me the space to independently explore complex biochemical problems, and I am grateful for his kind and patient mentorship. I also thank my committee members, Profs. George Garcia, Peter Scott, and Shaomeng Wang, for their patience, guidance, and support throughout my graduate career. I am thankful to them for taking time to meet with me and have thoughtful conversations about medicinal chemistry and science in general. From the Neamati lab, I would like to thank so many. First and foremost, I have to thank Shuzo Tamara for being an incredible, kind, and patient teacher and mentor. Shuzo is one of the hardest workers I know. In addition to a strong work ethic, he taught me pretty much everything I know and laid the foundation for the article published as Chapter 3 of this dissertation. The work published in this dissertation really began with the initial identification of PDI as a target by Shili Xu, and I am grateful for his advice and guidance (from afar!).
    [Show full text]
  • Investigating a Pathogenic Role for TXNDC5 in Tumors
    INTERNATIONAL JOURNAL OF ONCOLOGY 43: 1871-1884, 2013 Investigating a pathogenic role for TXNDC5 in tumors XIAOTIAN CHANG1, BING XU1, LIN WANG2, YAO WANG1, YUEJIAN WANG1 and SUHUA YAN1 1Medical Research Center of Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014; 2Research Center for Medicinal Biotechnology, Shandong Academy of Medical Sciences, Jinan, Shandong 250062, P.R. China Received June 29, 2013; Accepted August 2, 2013 DOI: 10.3892/ijo.2013.2123 Abstract. The expression of TXNDC5, which is induced by rs7771314, rs2815128, rs13210097 and rs9392182 between hypoxia, stimulates cell proliferation and angiogenesis. The cervical carcinoma, esophageal carcinoma and liver cancer increased cell proliferation, angiogenesis and hypoxia are main patients and controls. These results suggest that TXNDC5 has features of tumor tissues. The present study aimed to charac- increased expression in many tumors that is involved in the terize the expression of TXNDC5 in various tumor types and proliferation and migration of tumor cells, acting as a tumor- to investigate the role of TXNDC5 in the growth, prolifera- enhancing gene. The study also suggests that TXNDC5 gene tion and migration of tumor cells. The study also determined is susceptible to cervical carcinoma, esophageal carcinoma susceptibility of TXNDC5 gene on tumor risk. The expression and liver cancer risk. of TXNDC5 in tumor tissues was determined by immunohis- tochemistry using a tissue array that contained various types Introduction of tumor tissues.
    [Show full text]
  • Supplementary Table S4. FGA Co-Expressed Gene List in LUAD
    Supplementary Table S4. FGA co-expressed gene list in LUAD tumors Symbol R Locus Description FGG 0.919 4q28 fibrinogen gamma chain FGL1 0.635 8p22 fibrinogen-like 1 SLC7A2 0.536 8p22 solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 DUSP4 0.521 8p12-p11 dual specificity phosphatase 4 HAL 0.51 12q22-q24.1histidine ammonia-lyase PDE4D 0.499 5q12 phosphodiesterase 4D, cAMP-specific FURIN 0.497 15q26.1 furin (paired basic amino acid cleaving enzyme) CPS1 0.49 2q35 carbamoyl-phosphate synthase 1, mitochondrial TESC 0.478 12q24.22 tescalcin INHA 0.465 2q35 inhibin, alpha S100P 0.461 4p16 S100 calcium binding protein P VPS37A 0.447 8p22 vacuolar protein sorting 37 homolog A (S. cerevisiae) SLC16A14 0.447 2q36.3 solute carrier family 16, member 14 PPARGC1A 0.443 4p15.1 peroxisome proliferator-activated receptor gamma, coactivator 1 alpha SIK1 0.435 21q22.3 salt-inducible kinase 1 IRS2 0.434 13q34 insulin receptor substrate 2 RND1 0.433 12q12 Rho family GTPase 1 HGD 0.433 3q13.33 homogentisate 1,2-dioxygenase PTP4A1 0.432 6q12 protein tyrosine phosphatase type IVA, member 1 C8orf4 0.428 8p11.2 chromosome 8 open reading frame 4 DDC 0.427 7p12.2 dopa decarboxylase (aromatic L-amino acid decarboxylase) TACC2 0.427 10q26 transforming, acidic coiled-coil containing protein 2 MUC13 0.422 3q21.2 mucin 13, cell surface associated C5 0.412 9q33-q34 complement component 5 NR4A2 0.412 2q22-q23 nuclear receptor subfamily 4, group A, member 2 EYS 0.411 6q12 eyes shut homolog (Drosophila) GPX2 0.406 14q24.1 glutathione peroxidase
    [Show full text]
  • Transcriptomic and Proteomic Profiling Provides Insight Into
    BASIC RESEARCH www.jasn.org Transcriptomic and Proteomic Profiling Provides Insight into Mesangial Cell Function in IgA Nephropathy † † ‡ Peidi Liu,* Emelie Lassén,* Viji Nair, Celine C. Berthier, Miyuki Suguro, Carina Sihlbom,§ † | † Matthias Kretzler, Christer Betsholtz, ¶ Börje Haraldsson,* Wenjun Ju, Kerstin Ebefors,* and Jenny Nyström* *Department of Physiology, Institute of Neuroscience and Physiology, §Proteomics Core Facility at University of Gothenburg, University of Gothenburg, Gothenburg, Sweden; †Division of Nephrology, Department of Internal Medicine and Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, Michigan; ‡Division of Molecular Medicine, Aichi Cancer Center Research Institute, Nagoya, Japan; |Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden; and ¶Integrated Cardio Metabolic Centre, Karolinska Institutet Novum, Huddinge, Sweden ABSTRACT IgA nephropathy (IgAN), the most common GN worldwide, is characterized by circulating galactose-deficient IgA (gd-IgA) that forms immune complexes. The immune complexes are deposited in the glomerular mesangium, leading to inflammation and loss of renal function, but the complete pathophysiology of the disease is not understood. Using an integrated global transcriptomic and proteomic profiling approach, we investigated the role of the mesangium in the onset and progression of IgAN. Global gene expression was investigated by microarray analysis of the glomerular compartment of renal biopsy specimens from patients with IgAN (n=19) and controls (n=22). Using curated glomerular cell type–specific genes from the published literature, we found differential expression of a much higher percentage of mesangial cell–positive standard genes than podocyte-positive standard genes in IgAN. Principal coordinate analysis of expression data revealed clear separation of patient and control samples on the basis of mesangial but not podocyte cell–positive standard genes.
    [Show full text]
  • An Immunohistochemical Study of Thioredoxin Domain-Containing 5 Expression in Gastric Adenocarcinoma
    1154 ONCOLOGY LETTERS 9: 1154-1158, 2015 An immunohistochemical study of thioredoxin domain-containing 5 expression in gastric adenocarcinoma ZHIMING WU1,2, LIN ZHANG1, NAN LI1, LINA SHA1 and KUNPENG ZHANG1 1Department of Gastroenterology and Hepatology, The 309 Hospital of People's Liberation Army, Beijing 100091; 2Hebei North University, Zhangjiakou, Hebei 073000, P.R. China Received February 12, 2014; Accepted November 7, 2014 DOI: 10.3892/ol.2014.2832 Abstract. Thioredoxin domain-containing 5 (TXNDC5) is Introduction overexpressed in a number of human carcinomas. However, the involvement of TXNDC5 in gastric adenocarcinoma Gastric cancer is the most common malignant tumor world- remains unclear. In the present study, the immunohisto- wide (1,2). The most common pathological type of gastric chemical expression and clinicopathological significance of cancer is adenocarcinoma. Gastric adenocarcinoma is the TXNDC5 in gastric adenocarcinoma was investigated. The most common type of carcinoma in China. As a complex immunohistochemical expression of TXNDC5 was detected multifactorial process, gastric carcinogenesis is believed in 54 gastric adenocarcinoma specimens, and the correlation to involve numerous genes and their products (3-5). In our between TXNDC5 and the clinicopathological features was previous study, comparative proteome analysis revealed investigated. Of the 54 gastric adenocarcinoma specimens, that the expression of thioredoxin domain-containing 5 30 samples (55.6%) exhibited high TXNDC5 expression. In (TXNDC5) was significantly upregulated in certain precan- the adenocarcinoma specimens exhibiting high TXNDC5 cerous lesions of gastric cancer, such as varioliform gastritis expression, the proportion of poorly-differentiated adeno- (VG), compared with the peripheral normal gastric mucous carcinomas was significantly higher than that in specimens membrane (6).
    [Show full text]
  • Sulfiredoxin-1 Attenuates Injury and Inflammation in Acute Pancreatitis
    www.nature.com/cddis ARTICLE OPEN Sulfiredoxin-1 attenuates injury and inflammation in acute pancreatitis through the ROS/ER stress/Cathepsin B axis 1 2 3 4 1 5 1 1 1 Jun He , Miaomiao✉ Ma , Daming Li ,✉ Kunpeng Wang , Qiuguo Wang , Qiuguo Li , Hongye He , Yan Zhou , Qinglong Li , Xuyang Hou1 and Leping Yang 1 © The Author(s) 2021 Acinar cell injury and the inflammatory response are critical bioprocesses of acute pancreatitis (AP). We investigated the role and underlying mechanism of sulfiredoxin-1 (Srxn1) in AP. Mild AP was induced by intraperitoneal injection of cerulein and severe AP was induced by partial duct ligation with cerulein stimulation or intraperitoneal injection of L-arginine in mice. Acinar cells, neutrophils, and macrophages were isolated. The pancreas was analyzed by histology, immunochemistry staining, and TUNEL assays, and the expression of certain proteins and RNAs, cytokine levels, trypsin activity, and reactive oxygen species (ROS) levels were determined. Srxn1 was inhibited by J14 or silenced by siRNA, and overexpression was introduced by a lentiviral vector. Transcriptomic analysis was used to explore the mechanism of Srxn1-mediated effects. We also evaluated the effect of adeno- associated virus (AAV)-mediated overexpression of Srxn1 by intraductal administration and the protection of AP. We found that Srxn1 expression was upregulated in mild AP but decreased in severe AP. Inhibition of Srxn1 increased ROS, histological score, the release of trypsin, and inflammatory responses in mice. Inhibition of Srxn1 expression promoted the production of ROS and induced apoptosis, while overexpression of Srxn1 led to the opposite results in acinar cells.
    [Show full text]
  • Peroxiredoxins: Guardians Against Oxidative Stress and Modulators of Peroxide Signaling
    Peroxiredoxins: Guardians Against Oxidative Stress and Modulators of Peroxide Signaling Perkins, A., Nelson, K. J., Parsonage, D., Poole, L. B., & Karplus, P. A. (2015). Peroxiredoxins: guardians against oxidative stress and modulators of peroxide signaling. Trends in Biochemical Sciences, 40(8), 435-445. doi:10.1016/j.tibs.2015.05.001 10.1016/j.tibs.2015.05.001 Elsevier Accepted Manuscript http://cdss.library.oregonstate.edu/sa-termsofuse Revised Manuscript clean Click here to download Manuscript: Peroxiredoxin-TiBS-revised-4-25-15-clean.docx 1 2 3 4 5 6 7 8 9 Peroxiredoxins: Guardians Against Oxidative Stress and Modulators of 10 11 12 Peroxide Signaling 13 14 15 16 17 18 19 1 2 2 2 20 Arden Perkins, Kimberly J. Nelson, Derek Parsonage, Leslie B. Poole * 21 22 23 and P. Andrew Karplus1* 24 25 26 27 28 29 30 31 1 Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97333 32 33 34 2 Department of Biochemistry, Wake Forest School of Medicine, Winston-Salem, North Carolina 27157 35 36 37 38 39 40 41 *To whom correspondence should be addressed: 42 43 44 L.B. Poole, ph: 336-716-6711, fax: 336-713-1283, email: [email protected] 45 46 47 P.A. Karplus, ph: 541-737-3200, fax: 541- 737-0481, email: [email protected] 48 49 50 51 52 53 54 55 Keywords: antioxidant enzyme, peroxidase, redox signaling, antioxidant defense 56 57 58 59 60 61 62 63 64 65 1 2 3 4 5 6 7 8 9 Abstract 10 11 12 13 Peroxiredoxins (Prxs) are a ubiquitous family of cysteine-dependent peroxidase enzymes that play dominant 14 15 16 roles in regulating peroxide levels within cells.
    [Show full text]
  • The Cysteine Proteome 13 N Q114 Young-Mi Go, Joshua D
    Free Radical Biology and Medicine ∎ (∎∎∎∎) ∎∎∎–∎∎∎ 1 Contents lists available at ScienceDirect 2 3 4 Free Radical Biology and Medicine 5 6 journal homepage: www.elsevier.com/locate/freeradbiomed 7 8 9 Review Article 10 11 12 The cysteine proteome 13 n Q114 Young-Mi Go, Joshua D. Chandler, Dean P. Jones 15 Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Emory University, Atlanta, GA 30322, USA 16 17 18 article info abstract 19 20 Article history: The cysteine (Cys) proteome is a major component of the adaptive interface between the genome and 21 Received 17 November 2014 the exposome. The thiol moiety of Cys undergoes a range of biologic modifications enabling biological 22 Received in revised form switching of structure and reactivity. These biological modifications include sulfenylation and disulfide 23 17 March 2015 formation, formation of higher oxidation states, S-nitrosylation, persulfidation, metalation, and other Accepted 22 March 2015 24 modifications. Extensive knowledge about these systems and their compartmentalization now provides 25 a foundation to develop advanced integrative models of Cys proteome regulation. In particular, detailed Keywords: 26 understanding of redox signaling pathways and sensing networks is becoming available to allow the Cysteine proteome discrimination of network structures. This research focuses attention on the need for atlases of Cys 27 Redox proteome modifications to develop systems biology models. Such atlases will be especially useful for integrative 28 Redox signaling studies linking the Cys proteome to imaging and other omics platforms, providing a basis for improved 29 Functional network Thiol redox-based therapeutics. Thus, a framework is emerging to place the Cys proteome as a complement to 30 Free radicals the quantitative proteome in the omics continuum connecting the genome to the exposome.
    [Show full text]