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The Antibiotic Journey

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The Antibiotic Journey The Antibiotic Journey Zachary Bartow, Noah Quick, Alli Sanzari, Shereeka Brutis BSC 1005L-040 Life Science Lab April 18, 2018 http://www.smallworldinitiative.org Small World Initiative and Soil Microbes ● Find potential antibiotics in soil for antibiotic crisis ● Bacteria at an alarming rate becoming antibiotic resistant due overuse / resistant strains ● 14 countries, 38 states and Puerto Rico ● Mission : engage the youth in science and combat the antibiotic crisis ● Soil microbes - study of organisms in soil, their functions and http://birdcage3.blogspot.com/2013/11/a- how they affect soil properties misunderstood-form-of-art-agar-art.html ○ Ex : bacteria, fungi, algae, protozoa https://www.ien.com/supply-chain/news/20988510/us-craft-beer-industry-is-exploding http://www.lucyswhey.com/cateringplatters3/deluxe-cheese-platter http://www.cwwiebemedical Antibiotic Crisis and ESKAPE pathogens .ca/penicillin-truly-allergic/ ● Antibiotics - chemical that kills bacteria, inhibits the growth of microorganism ● Bacteria is becoming resistant to antibiotics due to overuse ● Pathogens are disease causing microorganism such as bacteria, virus, fungi, and protozoa ● ESKAPE pathogens - organism that are considered a major threat, make up the the majority of antibiotic resistant infections ○ ESKAPE pathogens ■ Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species ○ Safe relatives used in lab ■ Staphylococcus epidermidis, Escherichia coli, Erwinia carotovora, Acinetobacter baylyi, Bacillus subtilis, Enterobacter aerogenes, and Pseudomonas putida Bacteria Basics media Temp. Light or Isolate Gram Dark # stain (+/-) Zach BHI 25 Light AEA26 NA Noah BHI 25 Dark ADT6 + Alli LB 30 Dark MEE26 - Shereeka LB 30 Dark LRB9 + Results Student Own GPS CFU/G Gram Single Catalase Tryptophan Motility Lactose Hydrogen soil coordinates + or Colonies Production Metabolism (tail) Ferment Sulfide (bubble) ation Gram Production - Zach No 26.3832, - 4.9x10^7 NA Yes Yes No Yes Yes No 80.11464 Noah No 26 28’11’’N, 1.14x10^7 Yes Yes No Yes Yes No 80 8’56”W + Alli No 26.3832, - 3.24x10^7 Yes Yes No Yes Yes No 80.11464 - Sher No 26.370753, - 2.0x10^7 Yes Yes No Yes Yes No 80.101544 eeka + Macconkey Agar MacConkey growth Ferment lactose Match Gram Staining results? Zach yes yes No Noah yes yes Yes Alli yes yes Yes Shereeka yes yes Yes Antibiotic Activity Results Kills Resistant to Zach P. put Gram, Tri, Rif, Tet, P/S Noah B. sub, Gram, Tri, Rif, Tet, P/S P. put, Alli B. sub, Gram, Tri, Rif, tet, P/S P. put Shereeka N/A Gram, Tri, Rif, Tet, P/S DNA Sequence Results PCR Product and Organism with closest Percent Identity Sequence match to DNA Sequence Zach EEE-SWI27FHT Bacillus megaterium strain 99% Edgemont-009 16S ribosomal RNA gene, partial sequence Noah 10-ZBS-SWI27FHT Bacillus megaterium strain 98% Edgemont-009 16S ribosomal RNA gene, partial sequence Alli 37-UBB-SWI27FHT Bacillus thuringiensis strain Bt 99% 7 16S ribosomal RNA gene, partial sequence Shereeka MUH26S-P17- Pseudomonas sp. PONIH3 99% SWI27FHT chromosome, complete genome Conclusion Future Direction ●More interested in bacteria ● Bacteria is everywhere experiments; looking to keep up with ● Can grow from the smallest thing Yale bacteria studies ○ Streaking for single colonies ●Testing the ESKAPE pathogens in a secure lab Discussion ● Tested more single colonies (if any) ● Better original soil samples https://www.123rf.com/photo_21699425_scientist-or-professor-looking-around- a-blank-sign-with-advice.html .
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